JPS60125542A - Measured data correcting method - Google Patents

Abstract

PURPOSE:To improve the precision by measuring the concentration of the same liquid to be examined for correction with measuring wavelength light of individual items in case of colorimetric photometry of measuring items and using the measured conventration due to measuring wavelength light to correct data in every corresponding items in accordance with a specific calculating formula. CONSTITUTION:The luminous flux from a light source 1 is allowed to pass a lens 2, a stop 3, and a blank liquid 4 to be examined and is made incident on a photodetector 6 by a filter 5, which permits only prescribed wavelength light to pass through, and is converted photoelectrically. Data BLANK (lambda1-lambda7) obtained by measuring photoelectric conversion outputs corresponding to absorbancies of the blank liquid 4 to be examined to the respective wavelength light rays lambda1-lambda7 are stored in a memory together with measured data. Operations are performed for every measuring itsm in accordance with (the measured concentration of a material to be examined)-K.(the measured concentration of the blank liquid 4 to be examined) (K is a coefficient) and data of the blank liquid 4 to be examined which are measured with the same wavelength light are used to correct data of corresponding measuring items. Thus, the number of measuring items is increased, and high-precision corrected data are attained efficiently.

Description

DETAILED DESCRIPTION OF THE INVENTION [Technical Field] The present invention relates to a method for correcting measurement data in an automatic analyzer or the like that analyzes and measures body fluids such as serum by a colorimetric method.

[Prior art]

When colorimetrically measuring body fluids, chyle, jaundice, and hemolysis affect the measurement results, so in order to obtain reliable measurement data, it is necessary to detect their presence and eliminate samples that are unsuitable for measurement. It is necessary to remove them or measure their extent and correct them.

The correction method involves measuring the absorbance of a separate correction test solution (hereinafter referred to as "blank test solution") for each measurement item using light at a wavelength corresponding to the measurement item, and using this measurement data as the spectroscopic measurement data of the specimen. There is a method to correct by subtracting each from. Such a correction method requires a blank test solution or reagent for correction for each desired measurement item, and thus requires an extra sample in addition to the sample used for actual measurement. In addition, when this method is adopted for an automatic analyzer using a so-called multi-line method, which has measurement lines for each measurement item, the number of measurement items must be reduced by the demarcation line for the blank test solution, and one When this conventional method is adopted in a so-called single-line method in which a plurality of measurement items are measured on a measurement line, there are problems such as an additional measurement time required for blank test liquid measurement and a decrease in processing speed.

In addition, as another example of the conventional method, immediately after mixing the body fluid and reagent, measurement is performed using light of a wavelength corresponding to the measurement item to obtain correction data, and the final data obtained by photometry after the reaction is corrected using the correction data. There is a way. The disadvantage of this solution is
In many cases, adding the first reagent injected during photometry for correction data is insufficient to obtain the final data, so it is necessary to dispense the second reagent after obtaining the correction data. Not only is one measurement operation cumbersome, but the measurement items that can be corrected are limited. Furthermore, for measurement items in which a reaction occurs from the time of dispensing the first reagent, the reaction progresses between dispensing the first reagent and measuring the corrected data, making it difficult to obtain accurate corrected data. There is also. In addition, as a technique related to correction of measurement data, Japanese Patent Application Laid-Open No. 54-68785
There is a technology that has been fully described in the publication.

[Purpose of the invention]

An object of the present invention is to solve the various drawbacks of the above-mentioned measurement data correction method of an analyzer that measures a plurality of measurement items using a colorimetric method on a specimen. It is an object of the present invention to provide a method for correcting measurement data, which can be used to measure correction data of a small amount of sample, thereby allowing highly accurate and efficient correction of measurements of multiple items using a small amount of specimen.

[Summary of the invention]

In the measurement data correction method of the present invention, when carrying out colorimetric photometric measurements on a specimen for a plurality of measurement items, the concentration of the same correction test solution is measured using each of the wavelength lights used for measurement of each measurement item, and each measurement item is Using the measured concentration of the correction test solution using the wavelength light used for the measurement, for each corresponding measurement item, [measured concentration of the sample J-K] [measured concentration of the correction test solution], where K is the correction A coefficient determined by the influence that the attitude of the test liquid has on the measured concentration of the measurement item.

This method is characterized by correcting the measurement data of the specimen using the calculation formula.

〔Example 〕

Now, let the measurement items be A, B, C, D, and E, and measure each of the 4 items except E out of the 5 items using the 2-wavelength method 9 as shown in the following table, and the remaining E using the 1-wavelength method. Let's explain the case. Note that this condition applies to the central processing unit for controlling the self-axis analyzer (rOPIJ) to which the present invention is applied.
It's called J. ) is input to the OPU using the keyboard of the terminal.

〔table〕

For each measurement item in the above table, the data 'JFr:Aλ0. Aλ, Bλ, Bλ
2 Threat 8 ......Eλ.

On the other hand, the same correction test solution (blank test solution) is used to measure each measurement item, all wavelengths of light λ, λ2, λ8...
-BLANKλ, -BLA
NKλ, , BLANKλ8...BLAN
Obtain measurement data of Kλ.

Figure 1 is a schematic diagram showing an example of a configuration for measuring the concentration of the blank test solution using two wavelength or one wavelength light corresponding to the measurement items A to E in the table above.
A light beam from a light source 1 that emits light containing light of each wavelength for measurement is transmitted through a blank test liquid 4 through a lens 2 and an aperture 8', and only passes light of a predetermined wavelength according to the measurement item. The light passes through the filter 5 and enters the light receiving element 6, where it is photoelectrically converted.

The filter 5 filters light with a wavelength λ necessary for measuring the desired measurement item.
A plurality of filters are arranged on the rotating disk 7 corresponding to □ to λ, and by rotating the drive motor 8, T-phrase filters 5 that transmit only each wavelength light are sequentially inserted into the optical path. The photoelectric conversion output corresponding to the absorbance of the blank test solution 4 for each wavelength light λ□ to λ7 is sequentially measured, and BRANKλ, to
Obtain measurement data of BRANKλ.

FIG. 2 is a schematic diagram showing another configuration example for obtaining measurement data of BRANKλ, ~HRANKλ.

In this example, a diffraction grating 9 is used as a spectroscopic flat film for the light flux that has passed through the blank test liquid scratch, and each of the wavelengths of light λ□ to λ separated by this is received by a dedicated light receiving element 10, and is converted into a photoelectric converter. The absorbance of the blank test solution 4 for each wavelength light λ, ~λ is measured. In this figure, the same elements as those in FIG. 1 are denoted by the same reference numerals.

Measurement data BRANKλ ~ by each wavelength light λ□ ~ λ for one blank test solution obtained in this way
BRANKλ7 is measurement data Aλ□9Aλ, Bλ about wavelength light λ, ~λ7 for each measurement item of the object to be measured.
, Bλ...Stored in memory along with Fλ7! 8
By sequentially reading these and calculating the following formula for each measurement item, the sample measurement data of the corresponding measurement item can be obtained using the measurement data of the blank test solution measured with light of the same wavelength as the measurement wavelength of each item. Correct.

Measurement item A (Aλ, -Aλ2) - □ (BLANKλ□ - BLAN
Kλ, ), measurement item B (Bλ, -Bλ2) -K, (BI, ANKλ8-
BI, ANKλ2) Measurement summer negative eye 0 (0λ□−Cλ, ) −, (BLANKλ, −B
LANKλ,) Measurement item D (Dλ, -Dλ6) -K, (BLANK25- B
LANKλ6) Measurement item E Eλ7-6・BRANKλ.

In the above equation, Kl~ is a coefficient determined by the degree of influence that the blank test solution data has on each measurement item, and is set in advance. The arithmetic processing may be performed using the following.

In the above embodiment, it is clear from the principle of the uninvented method that +t\ can also be applied when the difference in the absorbance of the specimen mainly due to two wavelengths is used as measurement data.

Further, it is also possible to calculate sample information such as chyle, yellow stains, hemolysis, etc. from the measurement data of the blank test solution obtained by the method of the present invention. Therefore, λ in the above embodiment,
~λ7σ] If there is insufficient data in the measurement data using wavelength light σ), G'i, add the measurement wavelength yC suitable for calculating the sample information and measure the absorbance of the sample or blank test solution to the measurement wavelength light. Using the measurement data obtained by
[Effects of the Invention] As explained above in detail, the method of the present invention has the following effects.

(1) The processing speed can be improved by implementing the present invention in a self-axis analyzer (not just 'i' but multiple Line method 〇〇 Analyzer: 11, the number of measurement items can be increased compared to the conventional method. Since the measurement data obtained by using one common blank contact liquid is used as the correction data, only a small number of specimens and reagents are required, and therefore highly accurate correction data can be obtained without waste.

(3) By carrying out the method of the present invention using light of the wavelength necessary to obtain specimen information, it is possible to not only correct each measurement item but also calculate desired specimen information.

(4) It is possible to correct all measurement items without being restricted by measurement items or types of eyes.

(5) When applied to a type of analyzer equipped with a colorimetric photometer, it is possible to implement the method in an extremely simple manner by using some of the components of the colorimetric photometer. Since there is no need to separately provide a correction data measuring means in the reaction system, the analyzer itself can be downsized.

[Brief explanation of the drawing]

Figures 1 and 2 are schematic diagrams of different configuration examples for obtaining attitude measurement data using light of each wavelength corresponding to measurement items of a correction test liquid for carrying out the method of the present invention. 1... Light source 2 River lens δ... Aperture number... Correction test solution 5... Filter 6.10... Light receiving element? ... Rotating disk 8 ... Drive motor 9 ... Diffraction grating Patent applicant Olympus Optical Industry Co., Ltd. Figure 1 Procedural amendment March 6, 1981 1. Indication of the case 1988 Patent application No. 233545 No. 2 Name of the invention Measurement data correction method 3, person making the correction Relationship to the case Patent applicant (037) Olympus Optical Industry Co., Ltd. 1, Specification No. 2
In line 15 of the page, ``There is an extra sample in addition to the sample'' is corrected to ``In addition to the sample container, there is an amount of sample for medicine.'' 2. On page 8, line 1θ to line 16, "Send the final data."
...It is limited. [For measurement items that use two or more types of reagents, the amount of sample solution after injecting the first reagent is often less than the amount of sample solution required for measurement, which limits the number of measurement items. It is. Furthermore, if the amount of test solution is increased to enable photometry, the amount of reagent and sample used will be greater than necessary, which will increase running costs.'' 8. In the same page, page 11, line 11, "Analyzer" is corrected to "Analyzer". Procedural amendment January 16, 1985 1, Indication of the case 1988 ゛Patent Application No. 28fl1545 2 Name of the invention Measurement data correction method 3 Person making the amendment Relationship with the case Patent applicant (θ87) Olympus Kogaku Kogyo Co., Ltd. 1, Specification No. 7
“BRANKλ] on page 14th line and 16th line, ~BRA
NKλ,” is corrected to r BLANKλ, ~BLANKλ,” respectively. 2, page 8, line 8 [BRANKλ, ~BRANKλ
," is corrected to "BLANKλ, ~BLANKλ,]. 8. "Kλ, -K, -BRA" in the 8th line from the top of page 9 of the same page.
NKλ,” is corrected to “Eλ,-,・BLANKλ,”. From the 6th line to the 4th line from the bottom of page 10 of the same table [Automatic analyzer -
For 1111 analyzers, "" should be corrected to "If the processing speed can be improved by implementing the present invention in an automatic analyzer, or if the processing speed is the same as the conventional one."

Claims (1)

[Scope of Claims] 1. When carrying out colorimetric photometric measurements on the measurement items of the number of specimens, the axiality of the same correction test solution is measured using each of the wavelength lights used for the measurement of each measurement item, and the axiality of each measurement item is measured. Using the measured concentration of the correction test solution using the wavelength light used for the measurement, for each corresponding measurement item, [measurement concentration of the sample] - K [measurement of the correction test solution once] where K is the correction test solution. A coefficient determined by the influence of the test solution concentration on the measured concentration of the measurement item. A measurement data acquisition method characterized by correcting measurement data of a specimen using a calculation formula.