JPS58154513A - Preventive and remedying drug - Google Patents

Abstract

PURPOSE:To provide the titled drug containing the milk or milk component taken from an animal immunized with a virus proliferable in the digestive organ of man, as an active component, and useful for the prevention and remedy of diarrhea and hepatitis caused by the virus. CONSTITUTION:An aminal (e.g. cow) is immunized with a virus (e.g., rotavirus, hepatitis virus, etc.) proliferable in the digestive organs of man, and the milk taken from the animal, especially colostrum or its milk component is used as a component of the objective drug. Concretely, an animal of 6-9 months pregnancy is immunized with a virus (e.g. rotavirus, adenovirus, calicivirus, etc. for the remedy of diarrhea, and A-, B- and non-A and non-B-type virus for the remedy for hepatitis), by inoculating the amimal with the virus or virus vaccine subcutaneously or intramuscularly every 7-14 days for 3-6 times, the colostrum exudated after 1-3 days from delivery is collected, and the milk is used as it is or in the form of powdered milk as the preventive or remedy for the diseases preferably by oral administration.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to prophylactic and therapeutic agents. More specifically, the present invention relates to preventive and therapeutic agents for diseases such as diarrhea and hepatitis caused by viruses that proliferate in the digestive tract.

The prophylactic and therapeutic agent of the present invention is produced, for example, as follows.

That is, immunization is carried out by subcutaneously or intramuscularly inoculating a virus or virus vaccine 3 to 6 times every 2 to 1 day to a dairy animal that is 6 to 9 months pregnant. The amount of inoculation is not particularly limited, but the upper limit is determined by the side effects caused by inoculation.

As the virus vaccine, viruses or vaccines such as rotavirus and hepatitis virus are used. Preferably, the prophylactic and therapeutic agent of the present invention can be obtained by collecting colostrum 7 to 3 days after birth.

More specifically, the prophylactic and therapeutic agents for Jfl (especially infantile diarrhea) include human rotavirus, other adenoviruses, Karin virus (
calicivirus), Astro 1 virus (a
strovirus), coronavirus
avirus), minireovirus (m1nirθov
irus), balbovirus, and hepatitis prophylactic and therapeutic agents are produced from hepatitis A, B, or non-A, non-B hepatitis viruses. Note that a vaccine can also be used instead of a virus. The milk from dairy animals of the present invention, which has the effect of preventing diseases caused by viruses or alleviating or curing disease conditions, can be administered by any method, but oral administration is preferred as it allows for enteral absorption.

That is, milk as it is or its milk components, such as powdered milk obtained by dehydrating milk or dehydrating and skimming milk, is orally administered.

The dosage is determined according to the patient's age, health condition, weight, degree of disease, type of concurrent treatment, if any, frequency of treatment, nature of desired effect, etc.

Generally, the daily dose of milk is o, i -2θm
//ky body weight, usually about i y Oml/ky body weight, and is administered 7 or more times.

When the active ingredient of the milk of the present invention is orally administered, it is used in the form of tablets, capsules, powders, liquids, elixirs, and the like. When used in such forms, solid or liquid non-toxic pharmaceutical carriers can be included in the composition.

As an example of a solid carrier, conventional gelatin type capsules are used. The active ingredient may also be packaged as a tablet or powder, with or without adjuvants.

Next, examples will be described.

Example 1 Pregnancy! Human rotavirus (WA) in Holstein cows
strain)/θ9/mlV is subcutaneously immunized once every evening on the IO day. Colostrum will be collected on the 7th, 2nd and 3rd day after birth.

The obtained colostrum (1 ot) is centrifuged three times for 30 minutes at 70θ0 rotation to remove bacteria and fat, and the supernatant skim milk is collected. The anti-human rotavirus neutralizing antibody titers of the colostrum obtained on the first and second days are as shown in Table/.

Table/ Fruit bim 1 row Yumi Yu 2 oml of skim milk obtained on the first day in the same manner as in Example 1 was freeze-dried, and immediately before administration, it was added to distilled water λOm.
Dissolve in e and give 3 doses once every 7 days to patients with infantile dementia.
It was administered for days.

As a result, in the control group in which rotavirus colostrum was not administered,
Diarrhea continued for an average of days, but in the rotavirus colostrum administration group, diarrhea subsided within three days. Furthermore, when rotavirus in the stool was confirmed using an electron microscope, it was found that in the control group,
While rotavirus excretion continues for an average of 6 days, in the rotavirus colostrum administration group, rotavirus excretion stopped within 7 days, and in some cases, rotavirus excretion stopped within 7 days, confirming the effectiveness. It was done. Furthermore, no side effects due to the administration of rotavirus colostrum were observed in the rotavirus colostrum administration group.

Applicant Sendai Microbiology Research Institute Agent Patent attorney Hase - and others/names

Claims (1)

[Claims] fl. Prevention of diseases caused by viruses that proliferate in the human digestive tract, characterized by containing milk or its feather components collected from dairy animals immunized with viruses that proliferate in the human digestive tract. therapeutic drug. (2) Claim 1, characterized in that the virus is rotavirus and the disease is diarrheal disease.
Prophylactic and therapeutic drugs listed in Section 1. (3) The prophylactic and therapeutic agent according to claim 2, wherein the diarrheal disease is infantile diarrhea. (1) The prophylactic and therapeutic drug 1 according to claim 1, characterized in that the virus is a hepatitis virus and the disease is hepatitis. (5) A virus vaccine is used as the virus. A prophylactic and therapeutic agent according to any one of claims 1 to q. (6) Claims 1 to 2 are characterized in that pregnant dairy animals are immunized with a virus or a virus vaccine.
The prophylactic and therapeutic drug described in any one of the following items. (7) The prophylactic and therapeutic agent according to claim 6, wherein the milk is colostrum. (8) The prophylactic and therapeutic agent according to any one of claims 1 to 2, wherein the dairy animal is a cow.