JPS5616496A - Recovery of erythropoietin - Google Patents
Recovery of erythropoietinInfo
- Publication number
- JPS5616496A JPS5616496A JP9299979A JP9299979A JPS5616496A JP S5616496 A JPS5616496 A JP S5616496A JP 9299979 A JP9299979 A JP 9299979A JP 9299979 A JP9299979 A JP 9299979A JP S5616496 A JPS5616496 A JP S5616496A
- Authority
- JP
- Japan
- Prior art keywords
- erythropoietin
- urine
- ultrafilter
- salt
- anion exchanger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To recover the titled compound useful as a remedy for renal anemia, by concentrating and adsorbing erythropoietin in normal human urine with an ultrafilter and a basic anion exchanger, and eluting the erythropoietin with an aqueous solvtion of a salt.
CONSTITUTION: Normal urine or waste urine containing erythropoietin after extraction of a physiologically active substance, e.g. urokinase, is, without pretreatment, concentrated by an ultrafilter having a fractionating molecular weight of preferably 30,000 or less to a volume of 1/5W1/50 the original one. The resulting concentrate is adjusted to an electric conductivity of 0.5W8mmho/cm and pH4W8, and then brought into contact with a buffered basic anion exchanger to adsorb the erythropoietin. The adsorbed erythropoietin is washed with a buffer solution, and eluted and recovered with an aqueous solution of a salt, e.g. NaCl.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9299979A JPS5616496A (en) | 1979-07-20 | 1979-07-20 | Recovery of erythropoietin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9299979A JPS5616496A (en) | 1979-07-20 | 1979-07-20 | Recovery of erythropoietin |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS5616496A true JPS5616496A (en) | 1981-02-17 |
Family
ID=14070049
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9299979A Pending JPS5616496A (en) | 1979-07-20 | 1979-07-20 | Recovery of erythropoietin |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5616496A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0698019B2 (en) * | 1985-06-20 | 1994-12-07 | キリン―アムジエン(デラウエア)インコーポレーテツド | Protein purification |
US8193415B2 (en) | 1999-10-26 | 2012-06-05 | Stichting Dienst Landbouwkundig Onderzock | Plant expressing mammalian β1,4-galactosyltransferase and β1,3-glucuronyltransferase |
US8309795B2 (en) | 2001-01-19 | 2012-11-13 | Phyton Holdings, Llc | Method for secretory production of glycoprotein having human-type sugar chain using plant cell |
US8492613B2 (en) | 2002-03-19 | 2013-07-23 | Stichting Dienst Landbouwkundig Onderzoek | GNTIII expression in plants |
US8853370B2 (en) | 1998-12-09 | 2014-10-07 | Phyton Holdings, Llc | Plant-produced glycoprotein comprising human-type sugar chain |
US8927810B2 (en) | 2002-03-19 | 2015-01-06 | Stichting Dienst Landbouwkundig Onderzoek | Optimizing glycan processing in plants |
US9745594B2 (en) | 2007-04-17 | 2017-08-29 | Stichting Dienst Landbouwkundig Onderzoek | Mammalian-type glycosylation in plants by expression of a zebrafish glycosyltransferase |
-
1979
- 1979-07-20 JP JP9299979A patent/JPS5616496A/en active Pending
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0698019B2 (en) * | 1985-06-20 | 1994-12-07 | キリン―アムジエン(デラウエア)インコーポレーテツド | Protein purification |
US8853370B2 (en) | 1998-12-09 | 2014-10-07 | Phyton Holdings, Llc | Plant-produced glycoprotein comprising human-type sugar chain |
US8193415B2 (en) | 1999-10-26 | 2012-06-05 | Stichting Dienst Landbouwkundig Onderzock | Plant expressing mammalian β1,4-galactosyltransferase and β1,3-glucuronyltransferase |
US8907163B2 (en) | 1999-10-26 | 2014-12-09 | Stichting Dienst Landbouwkundig Onderzoek | Transgenic plants expressing galactosyltransferase and sialyl transferase |
US8309795B2 (en) | 2001-01-19 | 2012-11-13 | Phyton Holdings, Llc | Method for secretory production of glycoprotein having human-type sugar chain using plant cell |
US8735656B2 (en) | 2001-01-19 | 2014-05-27 | Phyton Holdings, Llc | Method of expressing galactosyltransferase and inhibiting xylosyltransferase or fucosyltransferase in a transgenic plant cell for secretory production of glycoproteins having human-type sugar chains |
US9574218B2 (en) | 2001-01-19 | 2017-02-21 | Phyton Holdings, Llc | Method of co-expressing galactosyltransferase and a glycoprotein in a transgenic plant cell and sialylating the glycoprotein for production of glycoprotein having human-type sugar chain |
US8492613B2 (en) | 2002-03-19 | 2013-07-23 | Stichting Dienst Landbouwkundig Onderzoek | GNTIII expression in plants |
US8927810B2 (en) | 2002-03-19 | 2015-01-06 | Stichting Dienst Landbouwkundig Onderzoek | Optimizing glycan processing in plants |
US9255277B2 (en) | 2002-03-19 | 2016-02-09 | Stichting Dienst Landbouwkundig Onderzoek | GNTIII expression in plants |
US9745594B2 (en) | 2007-04-17 | 2017-08-29 | Stichting Dienst Landbouwkundig Onderzoek | Mammalian-type glycosylation in plants by expression of a zebrafish glycosyltransferase |
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