JPS5596091A - Production of cells of actinomycetes microorganism - Google Patents

Production of cells of actinomycetes microorganism

Info

Publication number
JPS5596091A
JPS5596091A JP303879A JP303879A JPS5596091A JP S5596091 A JPS5596091 A JP S5596091A JP 303879 A JP303879 A JP 303879A JP 303879 A JP303879 A JP 303879A JP S5596091 A JPS5596091 A JP S5596091A
Authority
JP
Japan
Prior art keywords
actinomycetes
microorganism
pseudonocardia
cells
vitamin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP303879A
Other languages
Japanese (ja)
Other versions
JPS5653352B2 (en
Inventor
Teruo Watanabe
Hiroko Tajima
Tadao Matsubayashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kawamura Institute of Chemical Research
DIC Corp
Original Assignee
Kawamura Institute of Chemical Research
Dainippon Ink and Chemicals Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kawamura Institute of Chemical Research, Dainippon Ink and Chemicals Co Ltd filed Critical Kawamura Institute of Chemical Research
Priority to JP303879A priority Critical patent/JPS5596091A/en
Priority to GB8001601A priority patent/GB2042548B/en
Publication of JPS5596091A publication Critical patent/JPS5596091A/en
Publication of JPS5653352B2 publication Critical patent/JPS5653352B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H23/00—Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/365—Nocardia

Abstract

PURPOSE: One of actinomycetes microorganism which belongs to Pseudonocardia, assimilates methanol and can produce vitamin B12 is cultured in a culture medium containing methanol as the major carbon source to produce cells of Actinomycetes microorganism containing vitamin B12 and being useful as a protein source.
CONSTITUTION: An Actinomycetes microorganism which belongs to Pseudonocardia, assimilate methanol and produce vitamin B12, e.g., Pseudonocardia methanolignica (FERM-4747) or Pseudonocardia yukotis (FERM-4746), is cultured in a culture medium containing methanol as the major carbon source and a cobalt salt, e.g., cobalt chloride, at a temperature of 37W45°C and a pH about 6.5W7 for about 2W5 days. Thereby th cells of Actinomycetes microorganism containing a large amount of vitamin B12 accumulated are obtained in high yield.
COPYRIGHT: (C)1980,JPO&Japio
JP303879A 1979-01-17 1979-01-17 Production of cells of actinomycetes microorganism Granted JPS5596091A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP303879A JPS5596091A (en) 1979-01-17 1979-01-17 Production of cells of actinomycetes microorganism
GB8001601A GB2042548B (en) 1979-01-17 1980-01-17 Actinomycetes and process for producing vitamin b12 from microbial cells thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP303879A JPS5596091A (en) 1979-01-17 1979-01-17 Production of cells of actinomycetes microorganism

Publications (2)

Publication Number Publication Date
JPS5596091A true JPS5596091A (en) 1980-07-21
JPS5653352B2 JPS5653352B2 (en) 1981-12-18

Family

ID=11546132

Family Applications (1)

Application Number Title Priority Date Filing Date
JP303879A Granted JPS5596091A (en) 1979-01-17 1979-01-17 Production of cells of actinomycetes microorganism

Country Status (2)

Country Link
JP (1) JPS5596091A (en)
GB (1) GB2042548B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4430429A (en) 1981-07-23 1984-02-07 Wisconsin Alumni Research Foundation Production of vitamin B12 -activity substances

Also Published As

Publication number Publication date
GB2042548B (en) 1983-04-13
GB2042548A (en) 1980-09-24
JPS5653352B2 (en) 1981-12-18

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