JPH10236971A - Activated vitamin d enriched composition added with high amino acid component - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject composition capable of allowing a person to effectively ingest vitamin D by adding the homogenate of liver and kidney of poultry and fishes to a pulverized material of mushrooms, and stirring the pulverized material with the added homogenate at a specific temperature. SOLUTION: The objective composition is obtained by adding the homogenate of liver and kidney of poultry and fishes to a pulverized material of mushrooms (preferably Auricularia auricula), and stirring the pulverized material with the added homogenate at 25-40 deg.C, preferably 37 deg.C. Preferably, the mushrooms are pulverized to 10-100μm by a pulverizer, irradiated by ultraviolet rays of 295nm by a high-pressure mercury-vapor lamp for 10sec to 1min while shaking and stirring the pulverized mushroom, thereafter heated at 90-110 deg.C for 30min to 1hr, air-cooled and mixed with the homogenates. A freshwater eel is preferable as the poultry and the fishes. A mixing weight ratio of the mushrooms to the homogenates is preferably (1:1) to (1:5). When the homogenates are added, 5-10wt.% powder of Cinnamomi Cortex based on the weight of the mushrooms is preferably added thereto. Further, the stirring time is preferably 30-60min.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a highly amino acid-added active vitamin D enriched composition capable of efficiently ingesting vitamin D.

[0002]

2. Description of the Related Art In an aging society, prevention of osteoporosis has become a social problem in order to improve QOL of elderly women.
One of the important preventive measures is to increase bone mass during the growth period and to prevent future osteoporotic fractures in the long term. Bone mass depends on genetic factors, but important factors in environmental factors include pubertal nutrition and exercise habits.

The importance of calcium in adolescent nutrition is described in Am. J. Clin. Nutr., 52, 878-880 (199).
0) and N. Engl. J. Med., 327, 82-87 (1992). Today, interest in calcium intake is increasing, and the importance of calcium intake is recognized, especially for growing women, and inorganic calcium salts are being added to various foods and beverages. However, since absorption of calcium from the intestinal tract requires the presence of a binding protein, even if a large amount of inorganic calcium is taken, it is not sufficiently absorbed.

[0004] In 1993, the Fourth Edition Japanese Food Standard Composition Table was 30
A study conducted on 466 female high school students based on the added vitamin D ingredient table revised for the first time in a year (Vitamin, Vol. 70, No. 7, pp. 309-315, 1996)
), Calcium sufficiency was about 1/5 or less, whereas that of vitamin D was about 2/3 or less. And when the correlation with the amount of bone mineral was observed, it became clear that vitamin D intake was a more important factor than calcium intake. Therefore, in order to increase bone mass for prevention of osteoporosis and the like, vitamin D intake is extremely effective.

[0005] Vitamin D is effective as a healing factor for wilt disease, and it is known that irradiation of food or animal feed with ultraviolet rays produces an anti- wirl component. 1924 Steenboc
k is patented for irradiating foods such as fats and oils, butter and milk with ultraviolet light to increase anti-kulosis activity. Around the same time, Hess at Columbia University in the United States revealed that provitamin D, activated by ultraviolet radiation, was ergosterol, and converted the purified vitamin to vitamin D.
₂ and was named calciferol isolated at the same time by Askew of the University of Liverpool, UK. afterwards,
The 7-dehydrocholesterol vitamins generate a new time to ultraviolet radiation was named vitamin D _3.

[0006] In mammals, vitamin D ₂ and vitamin D ₃ behave exactly the same, and are generally described as vitamin D, and are collectively referred to as vitamin D in the present invention. In addition, the International Health Organization (WHO)
₃ as an international standard substance and 0.025 μg of crystalline vitamin D ₃
Is called one international unit.

On the other hand, mushrooms are known to have high contents of vitamin D and provitamin D. However, among these, provitamin D is immediately converted to other derivatives upon absorption into the body, and the amount of conversion of provitamin D to vitamin D by ultraviolet irradiation on the skin is extremely small (Kobayashi et al., J. Am. Nutr. Sci. Vitaminol.,
38, 15 (1992)), it was not possible to sufficiently absorb vitamin D by simply ingesting mushrooms.

[0008]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide an active vitamin D enriched composition that can efficiently ingest vitamin D.

[0009]

Under such circumstances, the present inventors have conducted intensive studies and as a result, crushed mushrooms, added a homogenate of liver and kidney of poultry and the like, and stirred to obtain vitamins. The present inventors have found that a highly amino acid-added active vitamin D enriched composition that can efficiently ingest D can be obtained, and completed the present invention.

That is, the present invention relates to a method of adding a poultry or fish liver and kidney homogenate to a crushed mushroom,
It is intended to provide a highly amino acid-added active vitamin D enriched composition obtained by stirring at 25 to 40 ° C.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION The mushrooms used in the present invention are not particularly limited as long as they are edible. For example, mushrooms, mushrooms, matsutake, maitake, hiratake, hattake, kotake, shimeji, Mushrooms and the like can be mentioned only as enoki, and especially jellyfish is preferable because of its high content of vitamin D. These mushrooms,
As such or dried one can be used.

In the present invention, first, a crushed product of mushrooms is obtained. The crushing method is not particularly limited, for example, it is preferable to use a crusher such as a stamp mill, a hammer mill,
Using these, 5-1000 μm, especially 10-100 μm
It is preferable to grind the fine particles into m fine particles.

The mushrooms thus pulverized can be mixed with a homogenate such as a liver as it is, but it is preferable to irradiate ultraviolet rays to convert the contained provitamin D into vitamin D. The ultraviolet irradiation is performed for 5 seconds to 30 while shaking and stirring the mushroom powder.
It is preferable to perform ultraviolet irradiation with a high-pressure mercury lamp, particularly preferably 295 nm, for 10 minutes to 1 minute, in which case water is sprayed for the purpose of preventing fine powder from flying up by shaking. It may be moistened. After irradiation with ultraviolet light, 80 to 120 ° C, particularly preferably 90 to 1
It is preferable to heat at 10 ° C. for 15 minutes to 6 hours, particularly 30 minutes to 1 hour, whereby an isomerization reaction of provitamin D and vitamin D can be caused to reach an equilibrium state. After the heating, it is preferable that the next step is carried out after cooling.

Next, the crushed mushrooms are mixed with poultry or fish liver and kidney homogenates. Here, poultry includes cows, pigs, chickens and the like, and fish include freshwater eels, carp, crucian carp, trout and the like. Of these, freshwater eels are particularly preferred. The homogenate can be used to prepare these livers and kidneys in 2 to 10 times, preferably about 3 times, a suitable buffer, for example, a phosphate buffer (pH
7.4), citrate buffer (pH 5.4) and the like, and then suspended using a homogenizer or the like. The mixing ratio of mushrooms and homogenate is
The weight ratio is preferably from 1: 0.5 to 1: 9, particularly preferably from 1: 1 to 1: 5.

[0015] At this time, it is preferable to further add a ginger powder or a cinnamon powder, because active vitamin D can be obtained in a higher yield and more stably. Of these, cinnamon powder is particularly preferred. Gennoshoko powder or cinnamon powder,
1 to 30% by weight, especially 5 to 10% by weight based on the weight of mushrooms
It is preferred to add by weight.

Next, the mixture is heated at 25 to 40 ° C., preferably 30 to 38 ° C., particularly preferably at about 37 ° C.
By incubating with stirring for 120 minutes, particularly preferably 30 to 60 minutes, a highly amino acid-added active vitamin D enriched composition can be obtained.

The thus obtained high amino acid-added active vitamin D enriched composition of the present invention can be used as it is or in the form of tablets, capsules, granules, powders, etc., in a conventional manner together with various additives. Or for medical use. Here, the various additives are not particularly limited as long as they are pharmaceutically acceptable pharmaceutical carriers. For example, lactose, sucrose, starch, calcium phosphate,
Excipients such as crystalline cellulose; binders such as starch, carboxymethylcellulose, anhydrous lactose; disintegrators such as starch; lubricants such as starch, talc, calcium stearate; other flavoring agents, flavoring agents, coloring agents, fragrances, etc. Is mentioned.

Highly amino acid-added active vitamin D of the present invention
The fortifying composition comprises 25-hydroxyvitamin D, 24,2
It is an active vitamin D enriched composition containing 5-dihydroxyvitamin D and 1α, 25-dihydroxyvitamin D. In addition, by mixing with a homogenate such as liver, it contains a large amount of essential amino acids such as valine, leucine, threonine, methionine, and tryptophan, and oligopeptides thereof, and the like. The amount can be increased. In particular, active vitamin D in a composition prepared by adding genoshoko powder or cinnamon powder has high activity and is stable.

[0019]

Industrial Applicability The high-amino acid-added active vitamin D enriched composition of the present invention contains active vitamin D, which is absorbed smoothly in a living body. It is extremely useful for increasing the amount of salt.

[0020]

EXAMPLES Next, the present invention will be further described with reference to examples, but the present invention is not limited to these examples.

Example 1 A commercially available dry jellyfish was crushed with a pulverizer (Mircer, Iwatani I).
FM-150), and the mixture was passed through a sieve having a sieve of 106 µm to collect the powder, and weighed 100 g. Spread it on a metal vat 30cm x 50cm wide, spray it with water and moisten it.
After leaving to swell for minutes, the mixture is evenly and uniformly irradiated with ultraviolet light from a high-pressure mercury lamp for 5 to 30 seconds while shaking and stirring. After irradiation with ultraviolet rays, the mixture is transferred to a beaker, heated on an oil bath, and heated at 100 ° C. for 30 minutes. After cooling, 300 g of a beef liver and kidney homogenate prepared in advance
Add. The homogenate solution was added to 50 g of fresh bovine liver and kidney under ice cooling in a volume 3 times the volume of the homogenate solution (0.19).
M sucrose, pH 7.4 phosphate buffer containing 1.87 mM magnesium acetate) is added, and the mixture is prepared using a homogenizer. To this is added 10 g of ginger powder powdered with a pulverizer, and the mixture is incubated at 37 ° C. with stirring for 90 minutes. The mixture was concentrated by a rotary evaporator and evaporated to dryness while blowing nitrogen to obtain a highly amino acid-added active vitamin D enriched composition.

Example 2 A commercially available dry jellyfish was crushed with a pulverizer (Mircer, Iwatani I).
FM-150), and the powder having passed through a sieve mesh of 106 μm is collected and weighed to 100 g. 200 g of a homogenate solution of beef kidney and liver prepared in advance
Add. The homogenate solution was added to 50 g of fresh bovine liver and kidney, respectively, under ice-cooling to a three-fold amount of the homogenate solution (0.1 g).
9M sucrose, pH 7.4 phosphate buffer containing 1.87 mM magnesium chloride) is added, and the mixture is prepared using a homogenizer. To this is added 10 g of ginger powder powdered with a pulverizer, and the mixture is incubated at 37 ° C. with stirring for 90 minutes. The mixture was concentrated by a rotary evaporator and evaporated to dryness while blowing nitrogen to obtain a highly amino acid-added active vitamin D enriched composition.

One hundredth of the reaction product thus obtained is transferred to a 100 ml Kolben, and 100 ml of a methylene chloride: methanol (1: 1) mixed solution is added in two portions and extracted. The mixture is concentrated under reduced pressure at a temperature of 40 ° C. or lower using a rotary evaporator, and finally solidified by blowing nitrogen. Sephadex L20 (manufactured by Pharmacia), which had been swollen overnight with a chloroform / normal hexane 65:35 mixed solution, was packed in a glass column with an inner diameter of 12 mm and a length of 17 cm with a reservoir to a height of 10 cm and a small amount. (1-2 ml) of the solidified product suspended in a mixed solution of chloroform / normal hexane 65:35 was added thereto, and fractionated by chromatography. At the end of the flow of approximately 10 ml of a developing solution of chloroform / normal hexane 65:35, 25-hydroxyvitamin D was discharged.
It is 24,25-
Dihydroxyvitamin D and 1α, 25-dihydrovitamin D eluted. When it is desired to accurately obtain a fraction of each vitamin D, it is preferable to flow each tritium-labeled vitamin D derivative and confirm the outflow position from its radioactivity.

The effluent thus obtained is concentrated using a rotary evaporator, and is evaporated to dryness by blowing nitrogen, and then redissolved in 1 ml of ethyl alcohol. The content of 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D was determined using a competitive binding assay using rat serum, and the content of 1α, 25-dihydroxyvitamin D was determined using a receptor extracted from bovine thymus. The receptor assay used was confirmed using the respective tritium labeled compound. In addition, 25-hydroxyvitamin D and 24,2
For the assay of 5-dihydroxyvitamin D, 0.1 ml of ethyl alcohol solution was used in duplicate, and 1α, 25-
For the assay of dihydroxyvitamin D, 0.3 ml was performed in duplicate. Since the radioactivity thus obtained was a competitive reaction, it was confirmed that the count number was always lower than that of the blank containing no active vitamin D.

Example 3 A highly amino acid-added active vitamin D enriched composition was obtained in the same manner as in Example 1 except that genoshoko powder was replaced by cinnamon powder.

Reference Example 1 How to make a vitamin D deficient chicken: A newborn white leghorn was raised on a vitamin D deficient chicken by feeding the following artificial synthetic diet. Vitamin-free casein, 240 g; cystine hydrochloride, 3
sucrose, 560 g; gelatin, 100 g; choline chloride, 2.3 g; cottonseed oil, 50 g; calcium hydrogen phosphate, 5.6 g; potassium dihydrogen phosphate, 15.5 g; calcium carbonate, 22.9 g; sodium chloride, 8 g; Mix 6.6 g of trace mixed salt; 2.1 g of vitamin mixture well. Composition of trace amounts of mixed salts: magnesium sulfate (heptahydrate),
745 g; manganese sulfate, 365 g; potassium iodate,
7 g; copper sulfate (pentahydrate), 2.2 g; zinc oxide;
1 g; sodium molybdate (dihydrate salt), 2.9
g; sodium selenite, 0.18 g; 0.2 g of iron citrate (hexahydrate). Composition of vitamin mixture: thiamine hydrochloride, 2.85 g; ribiflavin, 4.28 g; pantothenate calcium 9.5 g; nicotinamide, 23.7 g; pyridoxine, 3.8 g; biotin, 0.14 g;
475 g; folic acid, 0.95 g; vitamin B ₁₂ , 0.01
g; 477.78 g of sucrose is mixed well. In addition, chicks / weekly oil-soluble vitamins, β-carotene,
100 μg; α-tocopherol, 900 μg; 2-methyl-1,4-naphthoquinone, 120 μg, diluted in cottonseed oil and added to the diet. Oil-soluble vitamins are susceptible to oxidation and should be added weekly, and extra food supplemented with vitamins should be stored in the refrigerator. The above diet and water are available ad libitum and bred for 2-3 weeks to give chickens with typical vitamin D deficiency. The vitamin D-deficient chickens thus obtained are subjected to experiments 4 weeks later.

Reference Example 2 Preparation of vitamin D deficient rat: A vitamin D deficient rat was prepared by feeding a vitamin D deficient diet immediately after weaning and breeding for 3 to 4 weeks. Vitamin D deficient diet was purchased from Holtzman, Inc. of Madison, Wisconsin, USA.
This diet contains 0.47% calcium and 0.3% phosphorus.

Test Example 1 Four-week-old vitamin D-deficient chickens were allowed to freely eat a diet containing 1% by weight of the highly amino acid-added active vitamin D enriched composition obtained in Example 1 or 3. As a result, 4
After about 5 days, especially after one week, the foot was no longer wandering, the coat was improved, and she began to escape from the vitamin D deficiency symptom.

Test Example 2 Five-week-old vitamin D-deficient rats were divided into two groups of five rats.
Group A was continuously fed a diet containing 1% by weight of the highly amino acid-added vitamin D enriched composition obtained in Example 1, and group B was continuously fed a vitamin D-deficient diet. Two weeks later, the blood calcium concentration was measured by the OCPC method. As shown in Table 1, the rats in Group A showed normal values (4.3 to 5.
Returning to 2), group B remained hypocalcemic. Also, when a diet containing 1% by weight of the high amino acid-added active vitamin D enriched composition obtained in Example 3 was given, the blood calcium concentration returned to a normal value.

[0030]

[Table 1]

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Claims (6)

[Claims] 1. A highly amino acid-added active vitamin D enriched composition obtained by adding a homogenate of poultry or fish liver and kidney to a crushed product of mushrooms and stirring at 25 to 40 ° C. 2. After irradiating the crushed mushrooms with ultraviolet light,
A highly amino acid-added active vitamin D enriched composition obtained by heating, allowing to cool, then adding a homogenate of poultry or fish liver and kidney thereto and stirring at 25 to 40 ° C. 3. The highly amino acid-added active vitamin D according to claim 1 or 2, wherein a ginger powder or a cinnamon powder is added together with a liver and kidney homogenate of poultry or fish.
Reinforcement composition. 4. The mushroom is a jellyfish.
Highly amino acid-added active vitamin D according to any one of the above.
Reinforcement composition. 5. The composition according to claim 1, wherein the poultry or fish is a freshwater eel. 6. The high amino acid addition active vitamin D enriched composition according to any one of claims 1 to 5, which is for food or medicine.