JPH0943154A - Testing implement - Google Patents

Abstract

PROBLEM TO BE SOLVED: To rapidly and simply execute a test or an inspection by using one of a tube for sucking liquid sample by capillarity as a sample thief port, disposing a testing reaction reagent at a channel near the other opening, and completing the test reaction continuously in a blocked system. SOLUTION: The one opening of a tube 1 made of synthetic resin or glass having a fine channel 2 is used as a sampling port 3, and a testing reaction reagent 5 impregnated to filter sheet, woven fabric or nonwoven fabric is disposed at the channel 2 near the opening 4 of the opposite side. When the port 3 is brought into contact with liquid sample such as saliva, the sample is automatically sucked by capillarity, moved in the channel 2 to be brought into contact with the reagent 5, and reacted to be color developed or discolored, and hence the presence of specific ingredient (ingredient to be measured) can be recognized. Accordingly, in this testing implement, the labor hour of transferring the once collected sample to a test tube or other measuring instrument can be omitted, and it can be continuously, rapidly tested or inspected in the collected tube 1.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a test instrument capable of collecting a small amount of liquid sample and performing an immediate and simple test or inspection.

[0002]

2. Description of the Related Art Generally, a fixed amount of a liquid sample is
BACKGROUND ART Micropipettes and syringes are used as instruments to be collected for tests and inspections. Liquid samples collected using these instruments are introduced into the equipment or mixed with reagents in a test tube etc. for testing and inspection. It cannot be said to have sufficient convenience when an extra operation such as mixing is required and a test or inspection must be performed quickly. Further, there is a problem that such an operation becomes difficult when the amount of the liquid sample is very small.

Further, in order to save the trouble of collecting a sample, a method of impregnating a filter paper or the like with a test reaction reagent in a source of the sample to cause a reaction without the sample is also possible (a test paper such as litmus test paper). ), This method has a problem that the test reaction reagent dissolved from the filter paper or the like contaminates the sample collection source or exerts some influence on the sample collection source. That is, when saliva is tested or tested with a test paper such as a litmus test paper, the test can be easily performed by directly contacting it with the mouth, but depending on the type of test reagent, the reagent that enters the mouth may be a human body. There was a risk of harm.

In order to solve the above problem, for example, Japanese Patent Laid-Open No. 60-77739 discloses a micro-sampling device that uses a capillary tube to simplify the sampling. However, this sampling device is based on the premise that the collected sample is introduced into another measuring device for testing and inspection, and the purpose is to perform rapid testing and inspection in the sampling device. It wasn't.

From this point of view, the sample can be easily collected, the sample collection source is not contaminated with the test reaction reagent, and no additional operation such as mixing is required. A test method that completes the test reaction in a system occluded at the same time as collection is desired from the viewpoint of speed and safety.

[0006]

SUMMARY OF THE INVENTION The present invention has been made in view of the above problems, and an object thereof is to easily collect a small amount of liquid sample and continuously complete a test reaction in a closed system. This means that without affecting the sample source,
An object is to provide a test device that allows quick and easy testing and inspection.

[0007]

A test device of the present invention is formed of a tube having a fine flow path capable of sucking a liquid sample by a capillary phenomenon, and one opening of the tube serves as a sample collection port. The test reaction reagent is placed in the flow path near the other opening.

The cross-sectional shape of the flow channel is not particularly limited as long as it is a shape that can be sucked by a capillary phenomenon when a liquid sample is brought into contact with the sampling port, and is, for example, circular, elliptical, oval or triangular. It may have any shape such as a square, a rectangle, and a star.

When the flow path has a rectangular cross section, the short side has a dimension of about 50 to 800 μm and the long side has a dimension of 1.
0 mm or less is preferable.

The length of the above-mentioned tubular body is appropriately determined depending on the shape, size and use of the flow passage, but it is 0.8-10 m.
m is preferred.

Examples of the material of the tubular body include synthetic resin and glass. Examples of the synthetic resin include acrylic resin, styrene resin, hard vinyl chloride resin, polyethylene terephthalate resin, and polycarbonate resin, and transparent or translucent ones are preferably used.

The above-mentioned tubular body may be previously integrally formed into a predetermined shape by precision molding using the above-mentioned material, or may be produced by secondary processing from a plate material or the like of the above-mentioned material.

As shown in FIGS. 1 and 2, the test device of the present invention is formed from a tubular body 1 having a fine flow path 2. One opening of the tube body 1 is used as a sample collection port 3, and the flow path 2 near the opening 4 on the opposite side of the sample collection port 3 is used.
The test reaction reagent 5 is arranged in the. The test reaction reagent 5 is preferably used in a state of being impregnated with filter paper, woven fabric, non-woven fabric, or the like.

As the above-mentioned test reaction reagent, those which develop color or change color upon contact with a sample can be appropriately selected and used according to the purpose of use, and specifically, a pH reagent for measuring the pH of saliva. , A reagent that reacts with mercaptan in saliva or an alcoholic metabolite used for evaluating halitosis (eg, 4,4′-bisdimethylaminodiphenylcarbonium).

In the above test device, the sample collection port 3 of the tube body 1 is used.
Is brought into contact with the liquid sample, the sample is automatically aspirated by the capillary phenomenon, and the aspirated sample moves through the flow path 2 and comes into contact with the test reaction reagent 5 to react. The presence of a specific component can be confirmed by color development or discoloration caused by the reaction. Therefore, with this test device, it is possible to save the trouble of transferring the once-collected sample to a test tube, another measuring device, or the like, and it is possible to rapidly perform continuous tests and inspections in the collected test device.

In order to prevent the test reaction reagent 4 from coming into contact with a sample collection source (not shown) in the test instrument and affecting the sample collection source (not shown), a certain distance is provided between the sample collection port 3 and the test reaction reagent 4. It is preferable to place the sample (not shown) for this purpose so that the cross-sectional shape of the flow path 2 is, for example, a polygon with many corners, a star shape, a square with a plurality of grooves on its sides, or the like. It is preferable to substantially increase the distance to reach the test reaction reagent 4.

When the sample sucked from the sample collecting port 3 moves in the flow path 2 and the sample comes into contact with the test reaction reagent 5, the sample collecting port 3 is separated from the sample and the suction of the sample is stopped. , The total amount of the sample sucked into the channel 2
, And reacts with the test reaction reagent 5, the sample in the channel 2 does not flow back to the sample collection source, and the sample collection source is not affected by the test reaction reagent 5.

Further, when the test reaction reagent is placed at a position where the sample comes into contact with the test reaction reagent when the test sample is filled with the constant amount of the sample necessary for the test or inspection, a constant amount of the sample is always provided. It is possible to react with a test reaction reagent and obtain stable test results.

In the above-mentioned test device, as shown in FIG. 3, by providing a wide channel and arranging a plurality of types of test reaction reagents in parallel, a plurality of tests and It becomes possible to perform an inspection (for example, measurement of saliva pH and mercaptan) at the same time.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION

(Examples 1 to 8) Two acrylic resin plates having a thickness of 2 mm are opposed to each other, an acrylic resin thin plate having a predetermined thickness is inserted between them, and they are adhered with an adhesive agent. Eight types of 50 mm long tubular bodies having flow channels were prepared. When the sample solution (saliva) was brought into contact with one of the openings (sampling port) of these pipes, the sample solution was sucked into the flow channel, and the sample sampling port rose to the reach distance shown in Table 1. did.

[0021]

[Table 1]

(Examples 9 to 16) A pH test paper having a length of 10 mm was placed in the tube of each of Examples 1 to 8 from a sampling port.
The sample solution was set so that the lower end was located at a position 1 mm smaller than the reaching distance of the sample solution shown in Table 1. Then, when the sample solution (saliva) was brought into contact with the sample collection port of the tube, the sample solution reached the pH test paper and the pH test reaction was observed. At the same time that the sample solution reaches the pH test paper,
Even if the sampling port is disconnected from the sampling source,
From the observation of the test reaction, it is considered that there is no backflow of the test reaction reagent. As the pH test paper, a pH test paper having a length of 10 mm (“ADVANTE” manufactured by TOYO
C UNIV. pH 1-11 ") was used.

(Example 17) Three kinds of pH test papers ("TBB", "TB" and "MR" manufactured by Toyo Kagaku Sangyo Co., Ltd.) having different sensing ranges were placed inside the tube of Example 4. When the sample was placed in the same manner as in Example 9 and a suction test was performed on the sample in the same manner as in Example 9, the sample solution reached three types of pH test papers and a pH test reaction was observed.

(Example 18) A polyethylene terephthalate film having a thickness of 40 μm was bent into a fan shape having a width of about 2 mm, formed with a peculiarity, and then developed, and then rolled and rolled into a star-shaped tubular body (major axis: about 5 mm, minor axis: about 5 mm). About 2 mm). A pH test paper having a length of 10 mm was set so that the lower end was at a position 15 mm from the opening (sample sampling port) of the tube. When a sample solution (saliva) was brought into contact with this sampling port,
The sample solution reached the pH test paper and the pH test reaction was observed.

Example 19 A pH test paper having a length of 4 mm was set so that the lower end was located at a position 6 mm from one opening (a sampling port) of a glass tube having an inner diameter of 0.8 mm. When the sample solution (saliva) was brought into contact with this sample collection port, the sample solution reached the pH test paper and the pH test reaction was observed.

(Example 20) A pH test paper having a length of 10 mm was set so that the lower end was located at a position 3 mm from one opening (a sampling port) of a glass tube having an inner diameter of 2 mm. When a sample solution (saliva) was brought into contact with this sampling port,
The sample solution reached the pH test paper and the pH test reaction was observed.

[0027]

EFFECT OF THE INVENTION The test device of the present invention has the above-mentioned constitution, and since a small amount of liquid sample can be easily collected and the test reaction can be continuously completed in a closed system, it is quick and simple. In addition, it can be tested and inspected, and does not affect the sample source. Therefore, using the test device of the present invention, saliva can be directly sampled from the mouth, or direct water from a river can be sampled for testing and inspection.

[Brief description of drawings]

FIG. 1 is a schematic cross-sectional view showing an embodiment of the test device of the present invention.

FIG. 2 is a bottom view of the test device shown in FIG.

FIG. 3 is a schematic cross-sectional view showing another embodiment of the test device of the present invention.

[Explanation of symbols]

 1 Tubular body 2 Flow path 3 Sampling port 4 Opening 5 Test reaction reagent

Claims (1)

[Claims] 1. A test instrument formed from a tubular body having a fine flow path capable of sucking a liquid by a capillary phenomenon, wherein one opening of the tubular body serves as a sampling port and the other opening is formed. A test device in which a test reaction reagent is arranged in a nearby flow path.