JPH09304248A - Weighing apparatus and dilution apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a dilution apparatus hard to receive the effect of a previous sample even at a time of repeated use or multistage dilution and shortening a dilution time and simple to use. SOLUTION: A dilution mechanism S wherein a weighing pipe 10 having an opening at one end part thereof, a dilution liquid introducing pipe 20, having a first opening and closing valve 40 and a branch pipe 30 having a second opening and closing valve 50 are connected in a watertight and airtight state so as to internally communicate with each other is provided. A sample is introduced into the weighing pipe 10 and the branch pipe 30 in such a state that the first opening and closing valve 40 of the dilution mechanism S is closed and the second opening and closing valve 50 thereof is opened and, subsequently, the second opening and closing valve 50 is closed to hold the sample in the weighing pipe 10 and, thereafter, the first opening and closing valve 40 is opened to inject a dilution liquid into an preliminarily emptied dilution container 1 along with the sample held in the weighing pipe 10 to obtain a diluted sample.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a measuring device, a diluting device and a multi-stage diluting device, and more specifically, in a batch type automatic chemical analyzer, etc., a measuring device for measuring a predetermined sample and a predetermined dilution ratio of the sample. It relates to a diluter for diluting with.

[0002]

2. Description of the Related Art In an automatic chemical analyzer, a fixed amount of sample is usually measured and analyzed. Moreover, when measuring a high-concentration sample, it is necessary to dilute the measured sample to an appropriate concentration. Of these, when particularly large dilution is performed, so-called multi-stage dilution is performed in which the diluted sample is further diluted in order to improve the accuracy of the dilution rate.
Therefore, in the automatic chemical analyzer, the following diluting device is known as a device for weighing a sample and performing necessary dilution and multi-stage dilution.

FIG. 5 shows a diluting device according to the first prior art.
The dilution container 1, the sample meter 150, the pump 130, and the pressurized air introduction pipe 160 are provided. The diluting container 1 into which the sample to be diluted and the diluting liquid are successively injected has a motor 2
A stirring fan 3 that rotates at and a level sensor 5 are provided. Further, the sample measuring device 150 includes a measuring pipe 155 and two-way valves 152 to 154 provided close to both ends thereof.
It is composed of The three-way valves 140 and 141 are provided on the drain port L side of the dilution container 1 and the inlet side of the sample measuring device 150.
Is connected by a pipe line. Further, the compressed air introduction pipe 160 has a compressed air inlet R, and the two-way valve 142
Is interposed.

When diluting a sample with this diluting device, first, the sample inlet G side of the three-way valve 141 and the sample measuring device 150 are used.
With the side, the two-way valve 151 and the two-way valve 152 open, the pump 130 is operated to introduce the sample into the measuring pipe 155.
Then, when the two-way valves 153 and 154 are opened, the measuring pipe 1
The diluent is introduced from the diluent inflow port F into the dilution container 1 in an amount determined by the level sensor 5 while the sample measured by 55 is washed away. The dilution is completed by operating the motor 2 and stirring with the stirring fan 3.

The dilution rate can be set in a wide range by appropriately selecting the diameter and length of the measuring pipe 155, and even a single-step dilution can be a dilution rate of about 2 to 100 times. It is possible.

Here, in order to further dilute the diluted sample, the three-way valve 140 and the three-way valve 141 are connected to the dilution container 1 side.
Side, the measuring device 150 side of the three-way valve 141 and the three-way valve 140 side,
With the two-way valve 151 and the two-way valve 152 opened, the two-way valve 1
42 is opened, and the diluted sample in the dilution container is placed in the measuring pipe 15
5 is introduced under pressure. Next, when the two-way valves 153 and 154 are opened, the diluted liquid is introduced into the dilution container 1 by the amount determined by the level sensor 5 while the diluted sample measured by the measuring pipe 155 is washed away. This is a stirring fan 3
The second-stage dilution is completed by stirring with. Thereafter, by repeating the same operation, it is possible to dilute the third step or more.

However, in this diluting device, the sample and the diluted sample are measured by the measuring pipe sandwiched between the two-way valves, but the previous sample is likely to remain due to the complicated structure near the valve.
When used repeatedly, it causes a dilution error. This effect is particularly remarkable when performing multi-step dilution.

On the other hand, as a second conventional technique, an actual Kaihei 2-
There is a diluter shown in 81627. This diluter is shown in FIG. In FIG. 6, the dilution container 1 is provided with a stirring fan 3 rotated by a motor 2 and a drain valve 4. Further, a sample injection nozzle 106 and a diluent injection nozzle 104 are provided. This diluent injection nozzle 10
Reference numeral 4 constitutes an injection conduit for the diluent or the like together with the extra conduit length 105, and one end of the extra conduit length 105 communicates with the discharge side 102 of the three-way valve 100. The suction side 101 of the three-way valve 100 communicates with the diluent container 110, and the common side of the three-way valve 100 communicates with the syringe pump 120.

When diluting a sample with this diluting device, first, the three-way valve 100 is set to the suction side 101, and the diluting liquid is sucked from the diluting liquid container 110 into the syringe pump 120 via the common side 103. Next, the three-way valve 100 is connected to the discharge side 102.
Then, the diluting liquid in the syringe pump 120 is discharged to the side of the pipe extra length 105. Then, by repeating the above suction / discharge operation several times, the diluent suction nozzle 104 and the extra pipe length 105 are filled with the diluent.

Next, the diluting liquid in the diluting container 1 is drained via the drain valve 4, and then the sample is injected from the sample injecting nozzle 106 into the diluting container 1 until the lower end of the diluting liquid injecting nozzle 104 is immersed to some extent. Inject. Next, with the three-way valve 100 kept on the discharge side 102 side, the syringe pump 120 is sucked and the sample is introduced from the diluent injection nozzle 104. At this time, the introduced sample does not reach the syringe pump 120 by taking the sufficient length of the extra conduit length 105.

Then, after the dilution container 1 is emptied in advance,
The sample sampled by the diluent injection nozzle 104 and the extra pipe length 105 is discharged from the syringe pump 120 and injected into the dilution container 1. At this time, the amount of the sample to be injected is equal to the suction amount for one stroke of the syringe pump 120. That is, by suction of the syringe pump 120,
The sample is substantially weighed. Then, the syringe pump 120 is operated to inject the diluent through the three-way valve 100 a required number of times, and then the stirring fan 3 is stirred to complete the dilution.

In order to further dilute the diluted sample with this diluting device, the diluted sample is added to the syringe pump 120.
The sample is sampled into the dilution container 1 by the syringe pump 120 after being sucked from the dilution liquid injection nozzle 104 by using, and then the dilution container 1 is emptied. Then, in the same manner as described above, the diluting liquid is injected into the diluting container 1 a plurality of times and stirred to complete the second-stage dilution. Thereafter, by repeating the same operation, it is possible to dilute the third step or more.

In the case of this diluting device, the sample or the diluted sample to be rediluted does not reach the portion of the valve where the previous liquid is likely to remain. Further, the diluting liquid nozzle 104 into which the sample or the like is introduced and the extra pipe length are sufficiently washed with the diluting liquid during the subsequent injection operation of the diluting liquid. Therefore, unlike the first conventional technique, the dilution error does not occur due to the mixing of the previous sample or the diluted sample in the previous stage of the multi-step dilution.

However, in this diluting device, the amount of diluting liquid that can be injected in each suction / ejection operation of the syringe pump 120 is the same as the sampling amount of the sample. Therefore, in order to obtain a high dilution ratio, suction and discharge operations are performed many times, which takes time, and practically one-step dilution is limited to about 10-fold dilution.

[0015]

The present invention has been made in order to eliminate the above-mentioned drawbacks of the prior art. The purpose of the present invention is to prevent the sample from remaining and to prevent the previous sample from being repeatedly used. Another object of the present invention is to provide a weighing device that is not easily affected by the above and can be used easily. Another purpose is that the sample and the diluted sample at the previous stage are less likely to remain, and are less likely to be affected by the previous sample even during repeated use or multi-stage dilution, and the time required for dilution is high even at high magnification. It is to provide a diluting device that is short and can be used easily. Still another object is to provide a multistage diluter capable of performing efficient multistage diluting by combining a plurality of diluters.

[0016]

In order to achieve the above object, a measuring device according to a first aspect of the present invention is a pressurized fluid in which a measuring pipe having an opening at one end and a first opening / closing valve are interposed. The introduction pipe and the branch pipe in which the second on-off valve is interposed are connected to each other at a single connecting portion while maintaining mutual water-tightness and air-tightness. The sample in the measuring pipe and the branch pipe with the second opening / closing valve closed and the second opening / closing valve opened, and then the second opening / closing valve is closed to hold the sample in the measuring pipe, and then the first opening / closing valve is opened. By introducing the pressurized fluid into the measuring tube from the pressurized fluid introducing tube, the sample held in the measuring tube is discharged from the opening at one end of the measuring tube.

The diluting device according to a second aspect is the measuring device according to the first aspect, in which a diluting container is combined with a diluting mechanism that also functions as a diluting liquid injecting conduit. A diluting container into which the liquid was injected in tandem, a measuring pipe having an opening at one end, a diluting liquid introducing pipe having a first opening / closing valve, and a second opening / closing valve. A branch pipe and a diluting mechanism which are connected to each other in a single connecting portion in a state in which they can flow inside each other and are kept watertight and airtight, and close the first on-off valve and the second on-off valve. The sample is introduced into the measuring pipe and the branch pipe in the open state, and then the second opening / closing valve is closed to hold the sample in the measuring pipe, and then the first opening / closing valve is opened to hold the sample held in the measuring pipe. Diluted by injecting diluent into a pre-empted dilution container It is characterized in that it has a dilution means for obtaining a fee.

The multistage diluting device according to a third aspect of the present invention is a diluting container into which a sample to be diluted and a diluting liquid are sequentially injected, a first measuring pipe having an outlet at one end, 1
The first diluting liquid introduction pipe having the opening / closing valve and the sample inflow pipe having the second opening / closing valve are water-tight and air-tight in a single connecting portion. A first diluting mechanism connected in a maintained state; a second measuring pipe having an inflow / outflow port at one end; a second diluting liquid introducing pipe having a third on-off valve interposed; A branch pipe in which the on-off valve of 4 is interposed, and a second dilution mechanism which is connected in a single connecting portion in a mutually water-tight and air-tight state in which mutual flow is possible, and The sample introduced from the sample inflow pipe into the first metering pipe with the on-off valve closed and the second on-off valve closed is retained in the first metering pipe by closing the second on-off valve, and then The first on-off valve is opened to inject the diluent with the sample held in the first measuring pipe into a previously emptied dilution container. And the first diluted means for obtaining the first diluted sample by the above, and the first diluted sample introduced into the second measuring pipe with the third opening / closing valve closed and the fourth opening / closing valve opened. , The fourth on-off valve is closed to hold it in the second measuring pipe, and then the third on-off valve is opened to preemptively empty the diluent together with the first diluted sample held in the second measuring pipe. And a second diluting means for obtaining a second diluted sample by injecting into the diluted container.

The multistage diluting device according to claim 3 is capable of performing efficient multistage diluting by preparing a plurality of diluting devices according to claim 2 and combining them. However, regarding the diluting container as a component of each diluting device, a plurality of diluting devices share a single diluting container.

[0020]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 shows a weighing device according to an embodiment of the present invention. In the figure, a metering pipe 10 having an opening A at one end, a pressurized fluid introduction pipe 20 having a first opening / closing valve 40 interposed therein, and a branch pipe 30 having a second opening / closing valve 50 interposed therein. However, they are connected to each other at the connecting portion D in a state in which they can flow inside each other and are kept watertight and airtight.

When measuring a sample with this measuring device, first, the sample is introduced into the measuring pipe 10 and the branch pipe 30 with the first opening / closing valve 40 closed and the second opening / closing valve 50 opened.
At this time, the sample may be introduced either by suction from the opening A and discharged from the end C of the branch pipe 30 or from the end C of the 30 branch pipe and discharged from A. It is distributed from one of A and C to the other. Further, as the operation source for circulation, in addition to using the original pressure of the sample, a suction pump, a pressure pump, pressurized air, or the like,
Any one can be used. In the following description, it is assumed that all open / close valves not specifically mentioned are closed.

Then, after the whole of the measuring pipe 10 and the branch pipe 30 is filled with the sample, the second opening / closing valve 50 is closed in addition to the first opening / closing valve 40. This closing timing is controlled by the time after opening the on-off valve 50, and when the level of the sample flowing through the level sensor etc. is confirmed to be above a certain amount, or when the pressure change inside the tube is detected, the entire tube is closed. It can be managed based on the time when it is confirmed that the sample is full.

As a result, the flow between A to D to C is stopped, and the sample in the measuring pipe 10 and the branch pipe 30 is held as it is without flowing out from the opening A on the pressure balance.
The inner diameter of the measuring pipe 10 is not particularly limited, but in the case of a normal sample, it is desirable that the diameter is about 0.75 mm to 3 mm. If the inner diameter is larger than this, there is a risk that the pressure balance will be lost and the sample will fall down, depending on the viscosity of the sample.
If the inner diameter is smaller than this, the ratio of the amount of the sample attached to the inner wall of the measuring tube becomes larger than the inner volume, and there is a possibility that sufficient measuring accuracy cannot be obtained.

After that, when the first opening / closing valve 40 is opened to introduce the pressurized fluid into the measuring pipe 10 from the end B of the pressurized fluid introducing pipe 20, the sample held in the measuring pipe 10 is opened from the opening A to the measuring pipe. It can be discharged under pressure to the outside of 10. The amount of sample discharged at this time is equal to the internal volume of the measuring pipe 10 from A to D. Here, since the measuring tube is a simple tube, the measured sample does not remain, and it is possible to always measure a fixed amount. For example, in the case of a measuring tube having an inner diameter of 2 mm and a length of 16 cm, about 0.5 ml of sample can be measured.

Here, instead of interposing the on-off valves 40 and 50 on the pressurized fluid introducing pipe 20 and the branch pipe 30, it is conceivable to provide a three-way valve at the connecting portion D. However, in this case, as in the case of the conventional technique shown in FIG. 5, a valve is interposed at a position that divides the sample to be weighed, and the previous sample may remain and cause a dilution error during repeated use.

As the pressurized fluid, not only gas such as instrumentation air but also liquid such as diluting liquid can be used. When the diluting liquid is used as the pressurized fluid, it acts as a metering device and also as a diluting liquid injecting conduit. Further, in such a case, a diluting device described below can be provided by preparing a diluting container into which the discharged measured sample and diluting liquid are to be injected.

That is, FIG. 2 shows a diluting device according to an embodiment of the present invention. In the figure, a dilution container 1 and a dilution mechanism S are provided. The dilution container 1 is provided with a stirring fan 3 rotated by a motor 2, a drainage valve 4, and a level sensor 5. The diluting mechanism S is substantially the same as the diluting device of FIG. 1, and comprises a diluting pipe 10 having an opening A, a diluting liquid introducing pipe 20 ′, a branch pipe 30, a first opening / closing valve 40, and a second opening / closing valve 50. Has been done.

When diluting a sample with this diluting device, first, with the first opening / closing valve 40 closed and the second opening / closing valve 50 open, the sample is placed between the opening A and the end C of the branch pipe 30. The sample is introduced into the measuring pipe 10 and the branch pipe 30 by circulating the sample. In the case of FIG. 2, the sample is introduced from the end C, discharged from A, and circulated in such a manner as to be injected into the dilution container 1.

If the opening A is located at a position sufficiently inserted into the dilution container 1, it is possible to introduce the solution through the opening A and discharge it from the system C to the outside of the system. in this case,
It is necessary that the sample to be weighed is present in the dilution container 1 in advance and the liquid level thereof is appropriately above the opening A. The means for allowing the sample to exist in the dilution container 1 in advance is arbitrary,
In addition to the method of discharging the sample introduced from C from A by using the branch pipe 30 and the measuring pipe 10 and injecting it into the dilution container 1, a method of separately preparing a sample injection pipe and injecting it into the dilution container 1, etc. Can be considered. Further, as the operation source for carrying out the circulation, in addition to utilizing the original pressure of the sample, an arbitrary one such as a suction pump, a pressure pump or pressurized air can be used.

Next, in addition to the first opening / closing valve 40, the second opening / closing valve 50 is also closed. Thereby, the flow between A to D to C is stopped, and the sample in the measuring pipe 10 and the branch pipe 30 is held as it is without flowing out from the opening A on the pressure balance.

After that, when the first opening / closing valve 40 is opened in the diluting container which has been emptied in advance and the diluting liquid is introduced into the measuring pipe 10 from the end B'of the diluting liquid inflow pipe 20, the diluting liquid and the measuring pipe 10 are introduced. The sample held inside can be pressurized and discharged from the opening A to the outside of the measuring tube and injected into the dilution container 1. At this time, as in the case of FIG. 1, an amount of sample equal to the internal volume of the measuring pipes A to D can be accurately measured. As the operation source for introducing the diluent, any source such as a suction pump, a pressurizing pump, or pressurized air can be used in addition to the one using the original pressure of the diluent.

In the diluting device according to the present invention, the method for quantifying the diluting liquid is not particularly limited. That is, as shown in the figure, the level sensor 5 may be provided in the dilution container 1, and when the level sensor 5 detects the liquid level, the first on-off valve 40 may be closed to stop the injection of the diluent, or It is also possible to inject the measured diluting liquid with a measuring instrument or a syringe pump.
When the pre-measured diluting liquid is introduced from the empty diluting liquid inflow pipe 20, it is possible to inject the measured diluting liquid into the diluting container 1 by using compressed air. Also,
When it is not necessary to accurately measure the amount of diluting water as in the titration device, the diluting liquid of a substantially constant amount may be introduced by opening the first opening / closing valve 40 for a certain period of time.

FIG. 3 shows a multistage diluter according to an embodiment of the present invention. In the figure, the dilution container 1 and the first to third
The diluting mechanism S1, S2, S3 is provided. The dilution container 1 is provided with a stirring fan 3 rotated by a motor 2, a drain valve 4, and a level sensor 5.

The first dilution mechanism S1 includes a first metering pipe 11 having an outlet A1, a first diluent introducing pipe 21, a sample inflow pipe 31, a first opening / closing valve 41, and a second opening / closing valve. It is composed of 51. The second diluting mechanism S2 includes a second metering pipe 12 having an inflow / outflow port A2, a second diluting liquid introducing pipe 22, a first branch pipe 32, a third opening / closing valve 42, and a fourth diluting valve 42. It is composed of an on-off valve 52. Further, the third diluting mechanism S3 includes a third measuring pipe 1 having an inflow / outflow port A3.
3, third diluent introducing pipe 23, second branch pipe 33, fifth
The opening / closing valve 43 and the sixth opening / closing valve 53. In addition, the diluent introducing pipes 21, 22 and 23 are connected to the common pipe 28.
The branch points are B1, B2, and B3. Further, the first branch pipe 32 and the second branch pipe 33 have a branch point C.
2 and C3, a common conduit 5 in which a suction pump 6 is interposed
Joined 7

Although the detailed illustration of the diluting mechanisms S1 to S3 is omitted, the measuring pipe, the diluting liquid introducing pipe and the branch pipe (sample inflow pipe) are connected to each other at the connecting portions D1, D2 and D3 as in FIG. Is connected in a water-tight and air-tight state. The inflow / outflow ports A2 and A3 of the measuring tubes 12 and 13 are provided below the detection position of the level sensor 5 so that the diluted sample in the dilution container 1 can be sucked.

When diluting a sample with this multistage diluter,
First, a first diluted sample is obtained by the first dilution means. That is, with the first opening / closing valve 41 closed and the second opening / closing valve 51 opened first, the sample supplied from the end C1 is circulated from the sample introduction pipe 31 to the first measuring pipe 11. The source pressure of the sample can be used as a motion source for circulation, and a pump or the like can be used.

Next, the second opening / closing valve 51 is closed in addition to the first opening / closing valve 41. As a result, the flow of the sample is stopped and the sample in the sample introduction pipe 31 and the measuring pipe 11 is held as it is without further flowing out from the outlet A1 in terms of pressure balance. At this time, the diluting container 1 has an opening / closing valve 5
Since the sample that has flowed out from the measuring pipe 11 has been injected until the time 1 is closed, the drain valve 4 is opened and discharged to be emptied.

After that, the drain valve 4 is closed, the first opening / closing valve 41 is opened, and the diluent is introduced from the common conduit 28.
The sample held in the measuring tube 11 together with the diluting liquid is measured in the measuring tube 1
1 can be discharged under pressure and injected into the dilution container 1. The amount of the sample discharged at this time is A1 of the measuring pipe 11.
It is possible to measure a constant amount of sample, which is equal to the inner volume of D1 to D1.

The injection of diluent continues further after all the sample has been extruded. During this time, the sample adhering to the inner wall of the measuring tube is also washed away and poured into the dilution container 1. And
When the level sensor 5 detects the liquid level, the first on-off valve 4
Close 1 and stop injecting diluent. After that, the motor 2
Is operated and stirred by the stirring fan 3, the dilution is completed, and the first diluted sample is obtained. If the dilution water does not have a sufficient source pressure, it is necessary to properly install a pump or the like.

For example, the measuring pipe 11 has an inner diameter of 2 mm and is A1.
1 to D1 has a length of 16 cm and an internal volume of about 0.5 ml.
If the internal volume of the is 50 ml, a diluted sample of 100-fold dilution is obtained.

Next, the second diluted sample is obtained by the second diluting means. That is, first, by operating the pump 6 with the third opening / closing valve 42 closed and the fourth opening / closing valve 52 open, a part of the first diluted sample is discharged from the inflow / outflow port A2 of the measuring pipe 12. Suction and first branch pipe 32
Distribute to the outside via.

Next, the fourth opening / closing valve 52 is closed in addition to the third opening / closing valve 42, and the pump 6 is stopped. As a result, the flow of the first diluted sample is stopped, and the samples in the measuring pipe 12 and the branch pipe 32 are inflow / outflow port A due to pressure balance.
It is held as it is without flowing out of 2 and returning to the dilution container 1. At this time, the residual first diluted sample in the diluting container 1 is discharged by opening the drainage valve 4.
Empty.

After that, when the drainage valve 4 is closed and the third opening / closing valve 42 is opened to introduce the diluent through the common line 28,
The sample held in the measuring tube 12 together with the diluent can be pressurized and discharged from the inflow / outflow port A2 and injected into the dilution container 1. The amount of the sample discharged at this time is A2 of the measuring pipe 12.
It is possible to weigh out a fixed amount of the first diluted sample, which is equal to the internal volume from D to D2.

The injection of diluent continues further after all the first diluted sample has been extruded. During this time, the diluted sample adhering to the inner wall of the measuring tube is also washed out and injected into the dilution container 1. Then, when the level sensor 5 detects the liquid level, the third on-off valve 42 is closed and the injection of the diluent is stopped. After that, if the motor 2 is operated and stirring is performed by the stirring fan 3, the dilution is completed, and the second diluted sample is obtained.

In this embodiment, a third diluting means is further prepared. That is, in the same manner as the second diluting mechanism, the third diluting mechanism sucks and holds a certain amount of the second diluted sample from the diluting container 1 into the third measuring pipe 13, and then dilutes the diluting liquid into the second diluting liquid. Level sensor 5 with diluted sample of
The second diluted sample is weighed by injecting it into the dilution container 1 up to the detection position of, and a third diluted sample is obtained. By adding a similar dilution mechanism and using the same dilution means, further 4-step dilution, 5-step dilution, etc. are possible.

In the present embodiment, since a separate dilution mechanism is used for the dilution for each stage in this way, there is no possibility that a sample or a diluted sample in the previous stage is mixed and a dilution error occurs. However, since the inside of each measuring pipe is washed when the diluting liquid is introduced, it is possible to perform multi-stage dilution by repeatedly using a certain diluting mechanism a plurality of times. For example, the third dilution mechanism can be omitted and the second dilution mechanism can be used twice to perform three-step dilution.

It should be noted that the sample remaining in the dilution container 1 is negligible with normal dilution accuracy, but in order to obtain higher dilution accuracy, a dilution mechanism not used at that time is used. It is also possible to wash the inside of the dilution container 1. That is, after holding the sample or diluted sample in each measuring pipe, the sample in the diluting container 1 or the diluted sample is discharged by opening the drain valve 4, and at this time, the measuring pipe not used and The diluting liquid can be introduced into the diluting container 1 through the diluting liquid introducing pipe connected thereto for cleaning.

As another usage method, the dilution rate obtained by the second dilution mechanism and the dilution rate obtained by the third dilution mechanism are set to be different, and either one is selectively used. The dilution rate can be easily changed according to the sample concentration.

The dilution container 1 shown in FIGS. 2 to 4 is
Both can be used as a reaction tank for carrying out a color reaction or the like after dilution or as a cell for absorbance analysis. Also,
Although each of the above-mentioned operations is normally performed by a command from the external control device, it goes without saying that all or part of them may be manually performed.

[0050]

As described above, the weighing device according to the present invention is
With a simple structure, the sample is unlikely to remain, and is not easily affected by the previous sample even during repeated use, and can be used easily.
Further, the diluting device according to the present invention has a simple structure in which the sample is unlikely to remain, is less susceptible to the influence of the previous sample or the diluted sample of the previous stage even during repeated use or multi-stage dilution, and the time required for dilution is short. , And can be used easily. Further, the multistage diluter according to the present invention enables efficient multistage diluting by combining a plurality of diluters.

[Brief description of drawings]

FIG. 1 is a configuration diagram showing an embodiment of the present invention.

FIG. 2 is a configuration diagram showing an embodiment of the present invention.

FIG. 3 is a configuration diagram showing an embodiment of the present invention.

FIG. 4 is a configuration diagram showing an example of a conventional diluting device.

FIG. 5 is a configuration diagram showing another example of a conventional diluting device.

[Explanation of symbols]

 1 Diluting container 2 Motor 3 Stirring fan 4 Drain valve 5 Level sensor 6 Pump 10 Measuring pipe 20 Pressurized fluid introducing pipe 20 'Diluting liquid introducing pipe 30 Branch pipe 40 First opening / closing valve 50 Second opening / closing valve D Connection Department

Claims (3)

[Claims] 1. A metering pipe having an opening at one end, a pressurized fluid introduction pipe having a first opening / closing valve interposed therein, and a branch pipe having a second opening / closing valve interposed therein are integrated into a single unit. Samples are introduced into the metering pipe and the branch pipe in a state where the insides of the connecting parts are flowable and are connected in a watertight and airtight state, and the first opening / closing valve is closed and the second opening / closing valve is opened. Then, the second opening / closing valve is closed to hold the sample in the measuring pipe, and then the first opening / closing valve is opened to introduce the pressurized fluid from the pressurized fluid introduction pipe into the measuring pipe to hold it in the measuring pipe. A metering device for discharging the prepared sample from an opening at one end of the metering tube. 2. A diluting container into which a sample to be diluted and a diluting liquid are successively injected, a measuring pipe having an opening at one end, and a diluting liquid introducing pipe having a first opening / closing valve interposed therein. And a diluting mechanism in which the second opening / closing valve is interposed and the diluting mechanism is connected in a single connecting portion in a state in which the insides of the bifurcating parts can flow and are kept watertight and airtight. The sample is introduced into the measuring pipe and the branch pipe with the opening / closing valve closed and the second opening / closing valve opened, and then the second opening / closing valve is closed to hold the sample in the measuring pipe, and then the first opening / closing valve. A diluting device having a diluting means for obtaining a diluted sample by injecting a diluting liquid with a sample held in a measuring tube into a diluting container which has been emptied in advance. 3. A diluting container into which a sample to be diluted and a diluting solution are successively injected, and a first outlet having an outlet at one end.
Of the measuring pipe, the first diluting liquid introducing pipe in which the first on-off valve is interposed, and the sample inflow pipe in which the second on-off valve is interposed A first diluting mechanism which is connected in a water-tight and air-tight manner in which it can be distributed,
A second metering pipe having an inflow / outflow port at one end, a second diluent introducing pipe having a third opening / closing valve, and a branch pipe having a fourth opening / closing valve A second diluting mechanism in which the insides of the two connecting parts are flowable and are connected in a watertight and airtight state, and the first opening / closing valve is closed and the second opening / closing valve is opened. The sample introduced from the sample inflow pipe into the first measuring pipe is held in the first measuring pipe by closing the second opening / closing valve, and then the first opening / closing valve is opened and held in the first measuring pipe. The first diluting means for obtaining the first diluted sample by injecting the diluting liquid together with the prepared sample into the previously emptied diluting container, the third opening / closing valve is closed and the fourth opening / closing valve is opened. In the state, the first diluted sample introduced into the second measuring pipe is used for the second measurement by closing the fourth on-off valve. The second diluted by holding it in the tube and then opening the third on-off valve and injecting the diluent with the first diluted sample held in the second metering tube into a pre-empted dilution container. And a second diluting means for obtaining a sample.