JPH09243639A - Liquid intake-tape organic-substance detection apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a liquid intake-type organic-substance detection apparatus which does not require time and man power, which is small and lightweight and by which the mass of a target organic substance can be measured simply and quantitatively. SOLUTION: A card 1 is inserted into a body entrance 26, an opening part 2 and an opening part 8 are set in a position in which they agree, and a target organic substance in a fluid is captured on a mesh 3 by operating a fan 6. The card 1 is moved into a stage 7, a load 9 is lowered, a sheet of filter paper 10 and a film 11 come into contact with the mesh 3 on the opening part 2 for a definite time, and the target organic substance is taken out to the film 11 so as to be transferred. Then, a drum 15 is turned, and the film 11 is housed in a cleaning stage 14 so as to be cleaned with a cleaning liquid from a cleaning-liquid tank 12. The card 1 is placed again just under the load 9, the the sheet of filter paper is moved just under the load 9, the film 11 is lowered on it so as to come into contact for a definite time, and an antigen-antibody reaction is performed. After that, the film is cleaned, a coloring reaction is performed, the film is cleaned so as to be dried, and a determination operation by an optical measurement is performed.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to narcotics, opium, stimulants, hay fever allergens, carcinogens, explosives, bacteria,
The present invention relates to a fluid inhalation type organic substance detection device for inhaling a substance such as mold and mite allergen together with a fluid and quantitatively or qualitatively measuring the substance.

[0002]

2. Description of the Related Art Up to now, there are many methods for detecting organic substances by utilizing an antigen-antibody reaction, and the following examples have been reported. Example 1: Narcotics contained in the air to be detected adhering to and adsorbed on the filter are extracted with a solution, and the antibody is reacted with the antigen-antibody in the solution. Method for detecting type and the like (Japanese Patent Laid-Open No. 3-27446)
No. 0, No. 4-216456, No. 216460
Gazette, the same 216461 gazette). Example 2: A method of quantifying the amount of pollen by converting the amount of antigen incorporated into an antibody immobilized on a crystal oscillator into a change in frequency (Japanese Patent Laid-Open No. 4-315053). Similarly, a method using a surface acoustic wave element (JP-A-4-315054).
A method of forming a complex of an antigen and an antibody in agar and determining the amount of the antigen from the change in light transmittance (Japanese Patent Laid-Open No. 4-31)
8461 publication). Example 3: Method of knowing pollen amount by immunoblotting method (Yuichi Takahashi et al., Allergy, 12, 1612, (199
0)).

[0003]

The above-mentioned conventional example is a method of performing an antigen-antibody reaction in a solution. Such a conventional method has a problem that a large amount of solution such as water has to be used. Furthermore, there are problems that it takes a long time to measure and that liquid leakage occurs. Therefore, it is the current situation that it has not been widely put into practical use. Further, in the (Example 2), it is very difficult to eliminate the influence of substances other than the desired organic substance. Furthermore, the immunoblotting method of the above (Example 3) required a highly skilled technique.

In order to solve the above-mentioned conventional problems, it is an object of the present invention to provide an apparatus which is small in size, light in weight, convenient, and quantitative and capable of quantitatively measuring a target organic substance in a short time, labor and cost. To do.

[0005]

In order to achieve the above object, a fluid suction type organic substance detecting device of the present invention comprises a fluid suction means for sucking a fluid and at least a fragment containing a target organic substance in the fluid. Concentration means taken out from inside,
Organic substance fixing means for extracting and fixing the target organic substance from the fragment, labeled antibody fixing means for fixing labeled antibody to the target organic substance by antigen-antibody reaction, and removing means for removing unreacted labeled antibody in the antigen-antibody reaction And a coloring means for performing a coloring reaction involving the labeled portion of the labeled antibody,
At least a quantifying means for quantifying the amount of coloration due to the color reaction is provided.

In the above structure, the fluid is preferably air, exhaled breath, or physiological liquid. Further, in the above structure, it is preferable that the suction means has a flow path narrowed from the fan and the suction port, and the concentration means has a mesh structure. Further, in the above structure, the organic substance fixing means is preferably in the form of a film. Further, in the above configuration, the labeled antibody may be a labeled anti-narcotic, anti-pollen, anti-explosive, anti-bacterial, anti-fungal, anti-tick monoclonal antibody (monoclonal antibody) or polyclonal antibody (polyclonal antibody). preferable. Further, in the above-mentioned constitution, the labeled antibody preferably has an enzyme as a label. Further, in the above structure, the target organic substance is preferably a protein. Further, in the above-mentioned constitution, the target organic substance is a specific drug prescribed in the Narcotics Control Law, the Opium Law and the Stimulants Control Law, a pollinosis allergen,
It is preferably a carcinogen, explosive substance, bacteria, mold or mite. Further, in the above structure, the labeled antibody is
It is preferably fixed on a base material to be inserted into the input port of the detection device body.

Further, in the above structure, the base material is preferably hermetically stored. Further, in the above structure, it is preferable that the removing means uses water containing a surfactant.

Further, in the above construction, it is preferable that the coloring means contains an enzyme substrate. Further, in the above configuration, it is preferable that the quantification means is a means for electrically measuring and quantifying a change in intensity of at least one light energy selected from light in the ultraviolet region and light in the visible region by using a photoelectric element. Further, in the above configuration, it is preferable that at least one means selected from the concentration means, the labeled antibody fixing means or the coloration means is inserted into the main body including the fluid suction means and functions.

The fluid inhalation type organic substance detecting device of the present invention is for quantifying the target organic substance amount by measuring the coloration amount corresponding to the organic substance amount. That is, the amount of the antigen organic substance is known by immobilizing the labeled antibody on the organic substance as the antigen by the antigen-antibody reaction and measuring the amount of the antibody by the coloration method. By having the above-described configuration of the present invention, a system suitable for deviceization was constructed for the first time. For example, methamphetamine, morphine, heroin, cocaine, cannabis, marijuana, amphetamine, L
By using drugs such as SD as an antigen and using antibodies against them, the presence or absence and the amount of these drugs can be known. In addition, for example, the pollen contains an antigen protein that causes pollinosis (hay fever allergen), and the amount of pollen can be known by quantifying this antigen protein. For example, cedar,
Pollen such as cypress, ragweed, rice, pine, ginkgo, squid, mugwort and duckweed are targeted.

Further, the degree of contamination by airborne scattering of multidrug-resistant staphylococcus, which is required to be treated as a hospital infection, and the degree of contamination by allergens of mold and mite, which cause asthma, can be known by using these antibodies. You can For example, by using an organic substance that becomes an explosive substance such as 2,4,6-trinitrotoluene as an antigen and using an antibody that reacts with this as an antigen-antibody, it becomes possible to know the presence or absence and the amount of the explosive substance. .

[0011]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 shows a process process of a fluid inhalation type organic substance detecting device according to one embodiment of the present invention. The process steps shown in FIG. 1 will be described with reference to the apparatus configuration diagram shown in FIG. 2 and the process diagrams shown in FIGS. 3A and 3B. As shown in FIG. 2, the card 1 is used by inserting it into the main body entrance 26. The card 1 is sealed and sealed by a package film 27 as shown in FIGS. The card 1 has an opening 2. The mesh 3 is arranged so as to cover the opening 2, and the filter papers 4, 5 are arranged side by side.
Is provided. As shown in FIG. 2, the detection device main body 30
Includes a fluid suction port 29, a fan 6, a stage 7, an opening 8, a load 9, a filter paper 10, a film 11, a cleaning liquid tank 12,
A nozzle 13, a cleaning stage 14, and a drum 15 are provided.

When the target organic substance is attached to or contained in another supporting substance, the analysis is performed in the following order. First, when the card 1 is inserted into the detection device main body inlet 26, the card 1 is set at a position where the opening 2 and the opening 8 coincide with each other. In this case, the card may be moved together with the table on which the card is placed, or a moving mechanism may be used which moves the card while sandwiching the end of the card with a roll or the like. Then, the fan 6 starts operating,
The support material is sucked together with the fluid from the fluid suction port 29, and the support material is trapped on the mesh 3. This is collected (concentrated)
It is a process.

Next, the card 1 is moved through the gap between the heater 16 and the base onto the stage 7, and is set at a position where the opening 2 is located just below the load 9. And load 9
Is moved down, the filter paper 10 and the film 11 are brought into contact with the mesh 3 on the opening 2 for a certain period of time. As a result, a desired organic substance is taken out from the film captured on the mesh 3 to the film 11. This is a transfer process. Next, the load 9, the filter paper 10, and the film 11 are raised to their original positions, the drum 15 rotates, and the cleaning stage 14 comes directly below the load 9 from the position of FIG. 3A as shown in FIG. 3B. Then, the load 9, the filter paper 10, and the film 11 are lowered again, and when they are just accommodated in the cleaning stage 14, the cleaning liquid is sprayed from below toward the film 11 through the nozzle 13 from the cleaning liquid tank 12. This is the cleaning process.

After a certain amount of cleaning liquid is sprayed, the load 9, the filter paper 10 and the film 11 are raised again, the drum 15 is rotated in the reverse direction, and the stage 7 on which the card 1 is placed is again loaded with the load 9.
Right under. Thereafter, the card 1 slides in the direction perpendicular to the rotating direction of the drum 15, and the filter paper 4 comes directly under the load 9. The film 11 has come down onto the filter paper 4 and has been in contact therewith for a certain period of time. This step is the antigen-antibody reaction step. After that, the drum 15 is rotated, and the above-mentioned cleaning process is repeated again.

When the cleaning is completed, the drum 15 rotates in the reverse direction,
It returns to its original position, the card 1 slides, and the filter paper 5 comes under the load 9. Then, the load 9, the filter paper 10, and the film 11 have come down onto the filter paper 5 and are in contact therewith for a certain period of time. This step is the color reaction step. Then, the washing process is repeated again. Next, the drum 15 rotates in the reverse direction,
The card 1 returns to its original position, the load 9 rises, the film 11 is wound, and the portion where a series of treatments is performed is the heater 1.
Move up 6. Contact this heater 16 for a certain time,
Dried. This is the drying process. Further, the treated portion is moved to the measuring unit 28, where optical measurement is performed. This is the quantification process, which completes the measurement.

Next, the contents of each step will be described in more detail. (1) Collection step Although the fluid is sucked by the suction means, a method of simply providing an exposed portion to collect the fluid that naturally enters is also possible, but it is short by forcibly sucking the fluid by the fan 6 or the screw. It becomes possible to collect what makes the target organic substance necessary for detection in time. This sucked fluid passes through a filter etc. for removing dust, dust, etc.
In some cases it may be more appropriate to reach the mesh 3. Of course, the fan 1 may be a drum type, a propeller type, or the like. The fluid is preferably air, exhaled air, or physiological liquid (urine, sweat, tears, saliva, blood, semen, etc.), but is not limited to these. Mesh 3
It is necessary to change the eye size according to the size of the fragments to be collected. Fragments containing the target organic substance are captured by the mesh 3, but the mesh 3 can often be replaced with another substrate having a structure capable of ventilation. However, it is a matter of course that the present invention is not limited to this. Further, in order to make it easier to capture fragments or the like containing the target organic substance, it is often appropriate to apply an adhesive substance such as petrolatum in advance, but it is not limited to this. That is, for example, a glass substrate coated with an adhesive substance such as petrolatum may be projected into the fluid so as to capture fragments or the like containing the target organic substance on the glass substrate.

(2) Transfer step When the fragments are securely fixed on the mesh 3 so as not to be peeled off in the subsequent step, the mesh 3 can be used as it is in the subsequent step, but if it is not in terms of ventilation efficiency. There are many. In that case, it is appropriate in many cases to copy the target organic substance from the fragments collected on the mesh 3 onto the film 11 as another substrate. In this case, it is appropriate to use a method in which the film 11 pre-impregnated with the solution for extracting the target organic substance from the fragments is brought into contact with the fragments on the mesh 3 for a certain period of time. . This film 11
In some cases, for example, it may be appropriate to use a card-like form in which the card is inserted from the outside of the apparatus body into the insertion port of the apparatus body.
Incidentally, at least after this step, the film 11 needs to be kept in a wet state at all times. If necessary, it is appropriate to take a method of bringing a moist filter paper into contact with the film 11 and adding a liquid such as an aqueous solution as needed. Extracts are, for example, "useful immunoassays"
20% methanol, 2 as described on page 177
A mixture of 5 mM Tris-HCl and 192 mM glycine (pH 8.7) is suitable. For the film 11, a material to which an organic substance to be detected is easily immobilized, for example, a film of nitrocellulose, polyvinylidene fluoride or the like is suitable for detecting a protein. The film 11 is preferably porous or has irregularities on the surface. The pore diameter or the size of the unevenness is, for example, to enable immobilization of a protein having a molecular weight of tens of thousands,
The optimum value is 0.1 μm or more and 1 μm or less, but if the size is such that transparency can be maintained, transmitted light can be used as the final means for quantifying the coloration amount. Further, it is appropriate that the film 11 is pretreated by impregnating it with water, methanol or the like so that the extract can easily penetrate.

(3) Washing process As the washing liquid, for example, a surfactant (Tween-20) is used.
And the like) are suitable. In this step, so-called blocking effect can be obtained at the same time. In other words, blocking means immobilizing a molecule such as a protein at a site to which an antigen is not attached to prevent the labeled antibody from adhering nonspecifically in the subsequent antigen-antibody reaction, which results in unreacted This has the effect of facilitating the subsequent washing step of removing the labeled antibody. Of course,
The protein used in this case must be a substance that does not participate in the subsequent antigen-antibody reaction. As an example of the cleaning method, there is a method of spraying the cleaning liquid toward the side of the film 11 on which the antigen is attached. At this time, for example, it is suitable to carry out at a position other than the position where the transfer step, the antigen-antibody reaction step, etc. are carried out so that the washing solution does not scatter and spread. Then, after cleaning the card, the cleaning liquid needs to flow out from that position and collect in a specific place.

As another example of the cleaning method, a method of adding a cleaning liquid onto the film 11 while the film 11 is in contact with a water-absorbing (polymer etc.) substance is suitable. In addition, the cleaning efficiency can be further improved by adopting a system in which the film 3 and the water-absorbing substance relatively move in the state of rubbing. These allow a small amount of cleaning liquid. Further, as another example, a method of sandwiching it in a sandwich shape in a base material which has been impregnated with a cleaning liquid in advance and moving the film can be considered, but the method is not limited to these.

(4) Antigen-antibody reaction step In this step, the labeled antibody is immobilized on the antigen. That is, by contacting the film 11 and the filter paper 4 for a certain period of time, a complex is formed with the labeled antibody and the antigen. The labeled antibody needs to be present in and on the surface of the filter paper 4, but it is appropriate that the labeled antibody or a solution containing the labeled antibody is spread on the filter paper 4 immediately before this step. Examples of the method include, but are not limited to, a method of enclosing a labeled antibody or a solution containing the labeled antibody in a microcapsule or the like and breaking the encapsulation on the filter paper 4. Another method is to fix the labeled antibody on the card 1. As the method of fixing, a method of attaching the labeled antibody itself, a method of containing a solution in which the labeled antibody is dissolved in a substrate such as filter paper 4, and encapsulating the labeled antibody or a solution containing the labeled antibody in a microcapsule or the like,
A method of mounting it is suitable. Of course, if necessary, a preservative may be added.

The label of the labeled antibody may be any substance that can participate in the color reaction, and for example, an enzyme is suitable. When an enzyme is used as a label, it is reacted with a substance capable of decomposing the enzyme, and the amount of the decomposition is measured to determine the amount of the antibody. To measure the amount of decomposition, for example, it is appropriate to perform a color reaction involving a substance obtained as a result of decomposition and measure the amount of color. For example, there is a method in which an enzyme that decomposes a phosphate ester is attached as a label to an antibody, the phosphate ester is reacted, the resulting alcohol is subjected to a color reaction, and the amount of the color is measured to determine the amount of the antibody. In addition, when a monoclonal antibody is used as the antibody portion of the labeled antibody, the specificity is high, and therefore even a very small amount of organic substance can be measured. Similarly, when a polyclonal antibody is used, a single antibody can detect a plurality of organic substances having similar specificities, that is, having similar three-dimensional structures, although the specificity is slightly lowered. Furthermore, polyclonal antibodies have the advantage that they are easy to produce and inexpensive.

(5) Washing Step The unreacted labeled antibody is removed by washing the film 11. In this step, it is appropriate to use the same washing solution as used in the step (3) in the same washing method.

(6) Color reaction step As a method, the film 1 is placed on the filter paper 5 to which the color liquid is fixed.
1 is contacted for a certain time. At this time, it may be appropriate to add a liquid such as an aqueous solution and then perform the color reaction so that the color liquid easily undergoes the color reaction. The coloring liquid needs to be in and on the surface of the filter paper 5, but it is suitable that the coloring liquid is spread on the filter paper 5 immediately before this step. Examples of the method include a method in which a coloring solution or a solution containing the coloring solution is sealed in a microcapsule or the like, and the sealing is broken on the filter paper 5, but the method is not limited to this. . Another method is to fix the coloring liquid on the card 1. As the fixing method, a method of adhering the coloring liquid itself, a method of incorporating a solution in which the coloring liquid is dissolved into a substrate such as filter paper 5, and a solution containing the coloring liquid or the coloring liquid in microcapsules or the like are used. A suitable method is to enclose it and put it on. Of course, if necessary, a preservative may be added. Further, when an enzyme is used in the labeling part of the labeled antibody, an enzyme substrate is most suitable as the coloring solution.

(7) Washing Step The washing solution must be one that does not dissolve the color point formed. In many cases, water is suitable because the dye, which is a component of the coloring point, is insoluble in water.
However, if the component of the color point is water-soluble, on the contrary, an organic solvent is suitable. In that case, it must be an organic solvent that does not dissolve or discolor the film.

(8) Drying Step In this step, the film 11 is dried so that the quantitative determination can be clearly performed in the subsequent quantitative determination step. As a drying method, for example, after roughly removing water with a filter paper 10, the heater 1 is used.
The method of contacting 6 is the most suitable. Another method is air drying. For air drying, it is appropriate to use the air that has passed through the fan 6 to operate.

(9) Quantitative Step For measuring the amount of coloration, it is optimum to irradiate light and measure the amount of absorption. At that time, it is optimal to use a photoelectric element. For example, it is conceivable that the colored portion is irradiated with light from an LED or the like, and the reflected light, scattered light, or transmitted light is captured by a photodiode. With these methods,
A current proportional to the amount of reflected light, the amount of scattered light, or the amount of transmitted light is generated. At that time, it is appropriate that the LED and the photodiode to be used have high sensitivity at least to a wavelength near the absorption wavelength of the coloring portion. Practically, a method of amplifying this current using an operational amplifier or the like and measuring the voltage value is suitable. At that time, it is easy and easy to understand that the method of using the numbers, letters, words, etc. for explaining the step by providing a certain threshold stepwise in the display of the result is not limited to this. .

[0027]

EXAMPLES Hereinafter, examples of actual measurement using the fluid inhalation type organic substance detection device of the present invention will be described.

1-1. Preparation of Labeled Antibody First, an anti-methamphetamine antibody was prepared according to the description in Japanese Patent Application Laid-Open No. 3-274460 and Second Biochemical Experiment Course, Volume 5, “Immunobiochemistry Research Method”, pages 1 to 25.

This antibody was labeled with alkaline phosphatase (Alp) in accordance with the description in "Bioimmunological Chemistry Research", Volume 107, Biotechnology Laboratory Course, pages 107-112, and Alp-labeled anti-2,4,6-trinitrotoluene was used. / Ethanol antibody was prepared.

1-2. Preparation of methamphetamine aqueous solution (50, 10, 1, 0.1 mg
/ L) was prepared. 1-3. Preparation of Card A card as shown in FIG. 5 was prepared. On the plate-shaped vinyl chloride resin card 17, vaseline 18 was thinly applied to the tip, and then a filter paper 19 impregnated with the labeled antibody was attached, and a coloring liquid (nitroblue tetrazolium / 5-bromo-4-chloro) was applied.
The filter paper 20 impregnated with (-3-indolylphosphate mixed solution) was attached.

1-4. The measurement fan was started and 2 ml of the sample was sprayed in front of the mouth of fan 6. Then, the voltage value after the series of steps described above was read. The graph of FIG. 6 shows the correlation between the 2,4,6-trinitrotoluene / ethanol concentration thus obtained and the voltage value. 2,4,6-trinitrotoluene /
It was found that the voltage value decreased as the ethanol concentration increased. This is because the coloration amount increases as the 2,4,6-trinitrotoluene / ethanol concentration increases,
Therefore, the absorptance of irradiation light is increased and the amount of scattered light is decreased.

1-5. Comparative measurement A comparative sample was prepared using a mixture of ragweed pollen, β-ionone (odorous substance), sand grains and tobacco ash. Using this sample, measurement was performed in the same manner as in 1-4, but the voltage showed the same value as the initial value and no change was observed. From this result, the fluid inhalation type organic matter detection device of the present embodiment is 2, 4,
It was confirmed that 6-trinitrotoluene could be selectively quantified.

2-1. Preparation of Labeled Antibody First, in the same manner as in 1-1, Japanese Patent Application Laid-Open No. 4-318461 and Second Biochemical Experiment Course, Volume 5, "Immunobiochemistry Research Method".
An anti-cedar pollen antibody was prepared according to the description on pages 1 to 25. This antibody was labeled with alkaline phosphatase (Alp) according to the description of Vol. 5, "Bioimmunochemistry", pages 107-112 and "Watanabe et al., Allergy, 41 (6) 1992." A labeled anti-sugi pollen antibody was prepared.

2-2. Preparation of Specimen Cedar pollen collected in advance was scattered in a predetermined 4 square millimeters on a filter paper, and this was used as a specimen. The number of pollen was counted in advance for each specimen by microscopic observation (50 times).

2-3. Production of Card A card as shown in FIG. 7 was produced in the same manner as 1-3. A stainless steel mesh 2 in which vaseline is thinly applied to the opening 22 on the plate-shaped vinyl chloride resin card 21.
3 (20 μm grid) was attached, filter paper 24 impregnated with the labeled antibody was attached, and filter paper 25 impregnated with the coloring solution was attached.

2-4. The measurement fan was started and 2 ml of the sample was sprayed in front of the mouth of fan 1. Then, the voltage value after the above-described series of steps was read. The correlation between the pollen number and the voltage value thus obtained is the graph of FIG. It was found that the voltage value decreased as the number of pollen increased. This is because the amount of coloration increased as the number of cedar pollen increased, which increased the absorptance of irradiation light and decreased the amount of scattered light.

2-5. Comparative measurement A comparative sample was prepared using a mixture of ragweed pollen, β-ionone (odorous substance), sand grains and tobacco ash. Using this sample, measurement was performed in the same manner as in 2-3, but the voltage showed the same value as the initial value, and no change was observed. From these results, it was confirmed that the fluid inhalation type organic matter detection device of the present example was able to selectively quantify the cedar pollen.

The cleaning liquid used above is Tween-
It was a phosphate buffered saline containing 0.05% of 20 and the coloring solution was (mixed solution of BCIP and NBT). Therefore, an LED of 592 nm and a silicon photodiode having a sensitivity wavelength of 320 to 730 were used for measuring the coloration amount.

Although examples of 2,4,6-trinitrotoluene and cedar pollen have been described above, the object is not limited thereto. In other words, methamphetamine, morphine, heroin, cocaine, cannabis, marijuana, amphetamine, LSD, cypress, ragweed, rice, pine, ginkgo, himesiba, mugwort, camogaya,
Even when Staphylococcus, mold, mite, etc. were prepared and used, the corresponding antibodies could be measured using this device. At this time, it goes without saying that one antibody that reacts with a plurality of antigens may be prepared and used.

[0040]

As described above, according to the fluid inhalation type organic substance detecting apparatus of the present invention, the fluid inhaling means for inhaling the fluid and the concentration for extracting at least the fragments containing the target organic substance in the fluid from the fluid. Means, an organic substance fixing means for extracting and fixing the target organic substance from the fragment, a labeled antibody fixing means for fixing a labeled antibody to the target organic substance by an antigen-antibody reaction, and removing unreacted labeled antibody in the antigen-antibody reaction By including a removing means, a coloring means for performing a color reaction involving the labeled portion of the labeled antibody, and a quantifying means for quantifying the amount of coloration by the color reaction,
It is possible to provide a device that is small in size, light in weight, and can easily and quantitatively measure the amount of a target organic substance without requiring time, labor, and cost. That is, it is compact and the amount of a desired organic substance can be easily quantified in a short time.

The fluid suction type detection device of the present invention can be used not only as a single device but also as a device incorporated in other equipment. For example, by using an anti-pollen labeled antibody as a labeled antibody and installing this detection device in an air purifier, an air conditioner, a vehicle, etc., it is of course possible to quantify the amount of pollen, for example, even when not operating, It is also possible to add a function such that the operation of the operation for removing pollen is started when the amount exceeds a certain amount.

[Brief description of drawings]

FIG. 1 is a process diagram of a fluid suction type organic substance detection device according to an embodiment of the present invention.

FIG. 2 is a schematic diagram of a fluid suction type organic substance detection device according to an embodiment of the present invention.

3A and 3B are operation diagrams of a fluid suction type organic substance detection device according to an embodiment of the present invention.

FIG. 4A is a configuration diagram of a sealed card used in the fluid suction type organic substance detection device of one embodiment of the present invention. 9B to 9C are views showing the opening process.

FIG. 5 is a configuration diagram of a card used in the fluid suction type organic substance detection device according to the embodiment of the present invention.

FIG. 6 is a graph showing the characteristics of the fluid inhalation type organic substance detection device according to one embodiment of the present invention.

FIG. 7 is a configuration diagram of a card used in the fluid inhalation type organic substance detection device of one embodiment of the present invention.

FIG. 8 is a graph showing the characteristics of the fluid inhalation type organic substance detection device according to one embodiment of the present invention.

[Explanation of symbols]

 1 Card 2,8,22 Opening 3,23 Mesh 4,5,10,19,20,24,25 Filter Paper 6 Fan 7 Stage 9 Load 11 Film 12 Cleaning Liquid Tank 13 Nozzle 14 Cleaning Stage 15 Drum 16 Heater 17,21 Plate-shaped vinyl chloride resin card 18 Vaseline 26 Main body inlet 27 Package film 28 Measuring part 29 Fluid inlet port 30 Detector body

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Norio Mino 1006 Kadoma, Kadoma City, Osaka Prefecture Matsushita Electric Industrial Co., Ltd. (72) Koichi Kugimiya, 1006 Kadoma, Kadoma City, Osaka Matsushita Electric Industrial Co., Ltd. (72) Inventor Kazuo Yokoyama 1006 Kadoma, Kadoma City, Osaka Prefecture Matsushita Electric Industrial Co., Ltd. (72) Inventor Mamori Soga 1006 Kadoma, Kadoma City, Osaka Matsushita Electric Industrial Co., Ltd. (72) Inventor Nakagawa Tohru 1006 Kadoma, Kadoma-shi, Osaka Prefecture Matsushita Electric Industrial Co., Ltd. (72) Inventor Nagoya ▲ Taka ▼ 1300-12 Naka, Tsuchiura, Ibaraki Prefecture

Claims (14)

[Claims] 1. A fluid suction means for sucking a fluid, a concentrating means for taking out at least a fragment containing the target organic substance in the fluid from the fluid, and an organic substance fixing means for taking out and fixing the target organic substance from the fragment. A labeled antibody fixing means for fixing a labeled antibody to the target organic substance by an antigen-antibody reaction, a removing means for removing unreacted labeled antibody in the antigen-antibody reaction, and a color reaction involving a labeled portion of the labeled antibody. A fluid inhalation type organic substance detection device comprising: a coloring means for performing coloring and a quantifying means for quantifying a coloring amount by the coloring reaction. 2. The fluid inhalation type organic substance detection device according to claim 1, wherein the fluid is at least one fluid selected from air, exhaled breath, and physiological liquid. 3. The fluid inhalation type organic substance detection device according to claim 1, wherein the suction means has a flow path narrowed from a fan and a suction port, and the concentration means has a mesh structure. 4. The fluid inhalation type organic substance detecting device according to claim 1, wherein the organic substance fixing means is in the form of a film. 5. The fluid inhalation type organic substance according to claim 1, wherein the labeled antibody is at least one antibody selected from labeled anti-narcotics, anti-bacterial, anti-fungal, anti-mite monoclonal antibodies or polyclonal antibodies. Detection device. 6. The labeled antibody uses an enzyme as a label.
The fluid inhalation type organic matter detection device according to item 1. 7. The fluid inhalation type organic substance detection device according to claim 1, wherein the target organic substance is a protein. 8. The target organic substance is at least one selected from specific drugs prescribed in the Narcotics Control Law, Opium Law and Stimulants Control Law, hay fever allergens, carcinogens, explosive substances, bacteria, molds and mite allergens. Claim 1 which is a substance
The fluid inhalation type organic matter detection device according to item 1. 9. The fluid inhalation type organic substance detection device according to claim 1, wherein the labeled antibody is fixed on a base material which is put into the input port of the detection device main body. 10. The substrate is sealed and stored.
The fluid inhalation type organic matter detection device according to item 1. 11. The fluid inhalation type organic substance detecting device according to claim 1, wherein the removing means uses water containing a surfactant. 12. The fluid inhalation type organic substance detection device according to claim 1, wherein the coloring means contains an enzyme substrate. 13. The fluid according to claim 1, wherein the quantifying means is a means for electrically measuring and quantifying a change in intensity of at least one light energy selected from light in the ultraviolet region and light in the visible region by using a photoelectric element. Inhalation type organic substance detection device. 14. The fluid inhalation type organic substance detecting device according to claim 1, wherein at least one means selected from a concentrating means, a labeled antibody immobilizing means and a coloring means is inserted into a main body including a fluid inhaling means to function. .