JPH08101190A - Blood segregating device - Google Patents

Abstract

PURPOSE: To provide a blood segregating device which can segregate serum from blood simply and quickly with no use of any centrifuge and which allows omission of the operation to transfer the segregated serum to another test tube. CONSTITUTION: The blood segregating device is composed of a take-in hole 4 to suck a blood by capillary phenomenon, a passage 5 having a gap to admit passage of the serum of blood sucked in from the take-in hole 4, an air hole 6 furnished at the termination of the passage 5, and a serum dripping hole 7 provided between the passage 5 and air hole 6, and further, a test tube 3 is furnished which is for sampling the serum dripping from the hole 7 and is mounted in the position of the hole 7 removably.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood separation device,
In particular, it relates to a blood separation device for separating serum from whole blood.

[0002]

2. Description of the Related Art Conventionally, in blood tests, the collected blood is used as a sample of whole blood, red blood cells, plasma or serum depending on the purpose of the test. Especially, since serum mainly contains glucose such as glucose, cholesterol and triglyceride (neutral fat), it is used to measure blood glucose level and cholesterol in medical institutions such as hospitals. In order to extract such serum from whole blood, after collecting blood, the blood collecting needle is removed, the tip of the syringe is placed on the inner wall of the examiner, and the blood is gently poured. After being left to stand at room temperature for 30 to 60 minutes to completely coagulate the blood, it is centrifuged for 10 minutes in a centrifuge to collect the supernatant serum.

However, it takes about one hour to separate the serum from the whole blood, so that it is impossible to deal with the emergency test. In addition, the blood component obtained by such a blood separation method can obtain highly accurate and qualitatively excellent results, but it becomes difficult to handle the centrifuge and the like, and an expert familiar with the centrifuge etc. There was a problem that a blood test could not be performed without it.

[0004]

[Object] The present invention has been made to solve such conventional problems, and is a blood separation apparatus capable of separating serum from whole blood simply and in a short time without using a centrifuge. The purpose is to provide.

[0005]

The blood separating apparatus of the present invention which achieves the above object is a blood separating apparatus for separating serum from blood collected as a sample, and the blood separating apparatus sucks blood by capillary action. From an inlet, a passage having a gap for passing blood serum sucked from the intake, an air hole provided at the end of the passage, and a serum dropping hole provided between the passage and the air hole It consists of

In the blood separation device of the present invention,
A test tube for collecting the serum dropped from the serum dropping hole is detachably fixed to the position of the serum dropping hole. Furthermore, the blood separation device of the present invention is a blood separation device that separates the blood collected as a sample into blood cells and serum, and holds an inlet for sucking blood by a capillary phenomenon and blood guided from the inlet. A first translucent inspection window having a possible gap, a passage portion having a gap for passing serum of blood guided from the first translucent inspection window, and capable of holding serum guided from the passage portion The second transparent inspection window having a large gap and an air hole communicating with the second transparent inspection window.

In the blood separation device of the present invention,
The gap of the passage portion is formed in the range of 0.5 to 20μ, preferably 1 to 5μ or 0.5 to 0.9μ.

[0008]

After the puncture of an arm or the like, when the inlet of the apparatus is immersed in the blood spouted by the blood suction tool, the blood is sucked by the capillary action of the inlet and guided to the passage portion. Since the gap of the passage portion is formed in the range of 0.5 to 20 μ, for example, the serum mainly of the blood introduced from the intake can be passed, and the serum is dropped to the examiner from the serum dropping hole. To be done. By forming such a gap in the passage portion in the range of 1 to 5 μm, blood cells can be reliably removed. In addition, by forming it in the range of 0.5 to 0.9μ, it is possible to separate only serum from whole blood.

Further, the apparatus is provided with a first light-transmissive inspection window capable of holding blood sucked by the capillary phenomenon from the intake port and a second light-transmissive inspection window guided from the passage portion. Therefore, simultaneous measurement of blood cells and serum is possible by setting the inspection device equipped with a light emitting / receiving element for receiving and emitting light in the first transparent inspection window and the second transparent inspection window. You can

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the blood separation device of the present invention will be described below with reference to the drawings. As shown in FIGS. 1 (a) and 1 (b), the blood separation device 1 of the present invention includes a collection plate 2 for collecting blood, and a tester 3 for dropping the blood component collected by the collection plate 2. There is. Collection plate 2
Is an inlet 4 for sucking blood by a capillary phenomenon, a passage portion 5 having a gap for passing only blood serum sucked from the inlet 4, an air hole 6 provided at an end of the passage portion 5, and a passage. It is formed of a serum dropping hole 7 provided between the portion 5 and the air hole 6.

The inlet 4 forms a gap in the range of 100 to 200 μ in order to suck blood by the capillary phenomenon, but it is preferably 100 μ. Also, the passage part 5
The gap is 0.5μ to 20μ to allow serum to pass through.
It is formed in the range of. The size of red blood cells is about 5 to 7
μ, the size of white blood cells is about 7 to 15 μ, and the size of platelets is 1 to 2 μ. Therefore, by forming the gap of the passage portion 5 in the range of 1 to 5 μ, preferably 5 μ, it is possible to remove blood cells more reliably. It is possible to accurately measure blood sugar level and cholesterol. In addition, the gap of the passage portion 5 is 0.5 to 0.
By forming within a range of 9μ, preferably 0.9μ, and separating only serum from whole blood, blood glucose level, cholesterol, etc. can be measured more accurately.

The tester 3 is the serum dropping hole 7 of the collection plate 2.
It is detachably fixed to the position of the serum dropping hole 7 for the purpose of collecting from the serum dropping hole. Thereby, the serum separated from the whole blood can be collected by the examiner 3. Blood collection by the blood separation device 1 of the present embodiment configured as described above will be described below.

If blood sampling is performed to measure the blood glucose concentration, blood for collecting only serum in which the gap of the passage portion 5 of the sampling plate 2 is 0.9 μ in order to obtain the measurement accuracy. The separating device 1 is used. After puncturing an arm or the like, the blood drawn by a blood suction device (not shown) is dipped into the intake port 4 of the collection plate 2
Is sucked and guided to the passage portion 5. Since the gap of the passage portion 5 is formed with 0.9 μ, only the serum is allowed to pass through, and the serum is guided from the passage portion 5 to the serum dropping hole 7 and dropped on the examiner 3. After the blood serum necessary for the tester 3 is collected, the tester 3 is removed from the collection plate 2, so that the blood glucose concentration can be measured by a test reagent or the like.

In the above embodiment, the serum collected by the collecting plate is collected in the test tube for testing, but the invention is not limited to this, and it is used in the blood test apparatus 10 as shown in FIG. 2 (a). Blood may be held in the test card 11.
Such a blood test apparatus 10 includes a test card 11 for collecting and separating blood, and a main body 12 into which the test card 11 is inserted.

As shown in FIGS. 2 (a) and 3, the inspection card 11 has an inlet 1 for sucking blood by a capillary phenomenon.
3, a first translucent inspection window 14 having a gap capable of holding blood introduced from the intake 13, and a gap for passing blood serum introduced from the first translucent inspection window 14 A passage portion 15 having the second transparent inspection window 16 having a gap capable of holding the serum introduced from the passage portion 15;
And an air hole 17 communicating with the transparent inspection window 16.

The inlet 13 forms a gap in the range of 100 to 200 μ in order to suck blood by the capillary phenomenon, but it is preferably 100 μ. The gap between the first translucent inspection windows 14 is 1 to 200 for holding blood.
It is formed in the range of μ, but the size of red blood cells is 5 to 7 μ,
Since the size of white blood cells is 7 to 15 μ, it is preferable to form them in the range of 5 to 7 μ, preferably 7 μ for retaining red blood cells, and in the range of 7 to 15 μ, preferably 15 μ for retaining white blood cells.

The gap of the passage portion 15 is formed in the range of 0.5 μ to 20 μ in order to pass the serum mainly. Since the size of red blood cells is about 5 to 7 μ, the size of white blood cells is about 7 to 15 μ, and the size of platelets is 1 to 2 μ as described above, the gap of the passage portion 15 is in the range of 1 to 5 μ, preferably 5
By forming with μ to remove blood cells surely,
The translucent inspection window 16 allows more accurate measurement of blood glucose level, cholesterol and the like. In addition, the passage portion 15
The gap is in the range of 0.5-0.9μ, preferably 0.9μ
In this case, by separating only serum from whole blood, the blood glucose level, cholesterol, etc. can be measured more accurately in the second translucent inspection window 16.

The gap of the second translucent inspection window 16 is formed in the range of 0.5 to 5 μ in order to retain the serum. The gap of the second translucent inspection window 16 is in the range of 0.5 to 0.9 μ, preferably 0.9 μ in order to hold only the serum.
Is preferably formed. The first light-transmissive inspection window 14 and the second light-transmissive inspection window 16 are made of a light-transmissive material in order to transmit light from a light-emitting element included in the main body 12 described later. Become. Further, the first translucent inspection window 14 and the second translucent inspection window 16 are provided with lids 14A and 16A in which a test reagent corresponding to a blood component is accommodated in advance or a test reagent is printed. You may prepare.

As shown in FIG. 2 (b), FIG. 4 and FIG. 5, the main body 3 has an inspection card insertion port 21 for inserting an inspection card 11 filled with blood cells and serum, and this inspection card insertion. It is composed of a sensor unit 23 installed in the mouth 21 and a measuring unit 24 that inputs and calculates and processes signals of the sensor unit 23, and further includes a power supply unit 25 for the sensor unit 23 and the measuring unit 24. In a simple device, these are integrally provided in the case 33.

The sensor section 23 comprises two light emitting elements 26 for irradiating light into blood cells and blood serum, and two light receiving elements 27 for receiving light transmitted through blood cells and blood serum and outputting light receiving signals corresponding to blood components. The two light emitting elements 26 and the light receiving elements 27 are the first light-transmissive inspection window 14 and the second light-transmissive inspection window 16 of the inspection card 11 inserted into the inspection card insertion opening 21 at predetermined positions. Are juxtaposed against each other. Further, the two light emitting elements 26 and the light receiving elements 27 are provided in the first translucent inspection window 14 and the second light transmissive inspection window 14 of the inspection card 11.
Alternatively, they may be arranged so as to face each other through the translucent inspection window 16.

The measuring unit 24 measures the current signal from the light receiving element 27 and the previously determined absorptivity and blood components (eg red blood cells (RBC), white blood cells (WBC), glucose mainly glucose, cholesterol, triglyceride (neutral). (Fat) etc.) and a display device 29 for displaying the result of the calculation processing means 28 and the calculation processing means 28 for calculating the value of the blood component based on the calibration curve.

The arithmetic processing means 28 includes a storage circuit 30 for storing the data of the calibration curve and an arithmetic circuit 31.
The calculation circuit 31 calculates a light reception signal output from the light receiving element 27 to calculate a value corresponding to the absorption coefficient of the blood component, and further calculates the absorption coefficient based on the calibration curve stored in the storage circuit 30. , Find the value of blood component. That is, since the signal intensity of the received light signal corresponds to the intensity of the light received by the light receiving element 27, the arithmetic circuit 31 determines the voltage value (predetermined value) corresponding to the intensity of this signal and the light amount of the light emitting element 26. The absorbance of the blood component is obtained by taking the ratio of At this time, if necessary, calculation for correction is performed based on the characteristics of the element and the performance of the device.

For example, in the case of a blood glucose level obtained from the glucose concentration in serum, the calibration curve stored in the storage circuit 30 has wavelengths 400 to 1200 n of blood samples having different blood glucose levels in advance.
Blood glucose level x determined from the absorption spectrum in the m region
And a light absorption rate a of light of a predetermined wavelength (wavelength of light emitted from the light emitting element 13) are stored as a function x = f (a). Therefore, by plotting the change of the absorptivity depending on the blood glucose level for a predetermined wavelength, a calibration curve as shown in FIG. 6 can be obtained. The calibration curve thus obtained is obtained by, for example, approximating a blood glucose level of 0 to 200 mg / dl with a quadratic curve,
By approximating a blood glucose level between 200 and 1000 mg / dl as a straight line, it can be obtained as a function such as x = αa ² + βa + γ (x <200) x = ka + c (200 ≦ x) (where α,
β, γ, k, and c are predetermined coefficients obtained from the calibration curve).

Further, for blood components other than glucose, similarly to the above, each calibration curve is obtained, and each calibration curve is stored in the memory circuit 30. Each calibration curve stored in the storage circuit 30 can be selected by the selection switch 18 provided in the case 33 based on the inspection target. further,
The selection switch 18 is a first switch provided on the inspection card 11.
Translucent inspection window 14 or second translucent inspection window 16
It is possible to select either one of the two light emitting elements 26 that cause the light to be emitted to the light source. Thus, the blood cells and serum can be simultaneously measured by simply inserting the test card 11 filled with blood cells and serum into the test card insertion opening 21 of the main body 12.

The display device 29 is composed of a digital display device such as a liquid crystal display device. As shown in FIG. 5, the display screen 22 is provided on the upper surface of the case 33, and the value of the blood component obtained by the measuring section 24 is numerically displayed. Display as. The main circuit of the arithmetic processing unit 28 and the drive circuit of the display device 29 are provided on the substrate 34 and driven by the power supply unit 25. A blood test by the blood test apparatus 10 using the blood separating apparatus (test card 11) of the present embodiment configured as described above will be described below.

If this blood test is performed to measure blood cells and serum, the test card 11 has a gap of 15 μ in the first translucent test window 14 and zero gap in the passage portion 15. .9 .mu., And the second translucent inspection window 16 having a gap of 0.9 .mu. Is used. After puncturing an arm or the like, when the inlet 13 of the test card 11 is immersed in the blood spouted by the blood suction tool, the blood is sucked by the capillary action of the inlet 13 and guided to the first translucent inspection window 14. Get burned. Since the gap of the first translucent inspection window 14 is formed with 15 μm, blood cells can be held, and the blood from which the blood cells have been removed is guided to the passage portion 15. Since the gap of the passage portion 15 is formed with 0.9 μm, only the serum is allowed to pass through and the serum is guided to and held in the second translucent inspection window 16. At this time, air bubbles are generated in the gap between the first light-transmissive inspection window 14 and the second light-transmissive inspection window 16, but since the air hole 17 communicating with the inspection window 16 is provided, This bubble is transferred to the first transparent inspection window 14 and the second transparent window.
The light can be emitted from the transparent inspection window 16. As a result, blood cells can be uniformly filled in the gaps of the first translucent inspection window 14 and serum can be uniformly filled in the gaps of the second translucent inspection window 16.

The test card 11 filled with blood cells and serum is inserted to a predetermined position of the test card insertion port 21 of the main body 12 in which the power switch 19 is turned on in advance. At this time, the convex portion 11a formed on one side surface of the inspection card 11 turns on a drive switch (not shown) installed in the inspection card insertion port 21. At this time, the selection switch 18 selects the light emitting element 26 that irradiates the second translucent inspection window 16 filled with serum with light, for example.

Then, the selected light emitting element 26 is driven to irradiate the blood with a light having a predetermined wavelength and a certain amount of light, and the light reflected by the serum enters the light receiving element 27.
Outputs to the measuring unit 24 a signal having an intensity corresponding to the amount of received light. In the measuring section 24, the received light signal is read into the arithmetic circuit 31, and the arithmetic circuit 31 calculates the ratio between the received light signal and the voltage value corresponding to the intensity of the light emitted from the light emitting element 26, and also calculates a predetermined constant. Is subtracted to obtain the absorbance a of the serum. The arithmetic circuit 31 further performs an arithmetic operation on the obtained absorptivity a based on the coefficient of the stored calibration curve to obtain the blood glucose level x, and displays the blood glucose level x on the display screen 22 of the display device 29.

When the measurement is performed again, the reset button 20 may be pressed to reset the measuring unit 24.
By this reset, the measuring unit 24 measures the absorptance of the light emitted from the light emitting element 13, and after the predetermined calculation, redisplays the blood glucose level.

[0030]

As is apparent from the above embodiments, according to the blood separation device of the present invention, it is a blood separation device for separating serum from blood collected as a sample, and the blood is sucked by a capillary phenomenon. Inlet, a passage having a gap for passing the blood serum sucked from the inlet, an air hole provided at the end of the passage, and a serum dropping hole provided between the passage and the air hole Therefore, it is possible to separate serum from whole blood easily and in a short time without using a centrifuge, and a test tube for collecting serum to be dripped from the serum dropping hole has a serum dropping hole. Since it is detachably fixed to the position, the operation of transferring the separated serum to another examiner can be omitted.

Further, according to the blood separation device of the present invention,
A blood separating device for separating blood collected as a specimen into blood cells and serum, the first light transmitting device having an inlet for sucking blood by a capillary phenomenon and a gap capable of holding blood introduced from the inlet. Sex inspection window, a passage portion having a gap for passing serum of blood introduced from the first translucent inspection window, and a second translucency having a gap capable of holding serum introduced from the passage portion And the air hole communicating with the second light-transmissive inspection window, so that the first light-transmissive inspection window and the second light-transmissive inspection window receive and emit light. By setting it in an inspection device equipped with a light emitting and receiving element, the separated blood cells and serum can be measured simultaneously. This allows
Since the blood test can be simplified, automated, miniaturized, and speeded up, the practitioner who does not have a clinical laboratory or the patient himself / herself can easily perform the blood test.

[Brief description of drawings]

FIG. 1 is a view showing an embodiment of a blood separation device of the present invention,
(A) is a whole perspective view, (b) is an explanatory view of a use state.

2A and 2B are views showing another embodiment of the blood separation apparatus of the present invention, in which FIG. 2A is an overall perspective view and FIG.

FIG. 3 is a cross-sectional view taken along the line AA of the test card (blood separation device) shown in FIG.

FIG. 4 is a block diagram showing a configuration of a main body of a blood test apparatus in which the blood separation apparatus of the present invention is used.

FIG. 5 is a side sectional view of a main body of a blood test apparatus in which the blood separating apparatus of the present invention is used.

FIG. 6 is a calibration curve showing the relationship between blood glucose level and absorptance.

[Explanation of symbols]

 1 ... Blood Separation Device 2 ... Collection Plate 3 ... Test Tube 4 ... Intake Port 5 ... Passage Portion 6 ... Air Hole 7 ... Serum Droplet Hole 11 ... Test Card (Blood Separation Device) 13 ... Intake Port 14 ... First Permeation Optical inspection window 15 ... Passage portion 16 ... Second transparent inspection window 17 ... Air hole

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masanori Suzuki 8-20-2 Nishishinjuku, Shinjuku-ku, Tokyo Jam Sales Co., Ltd.

Claims (6)

[Claims] 1. A blood separating apparatus for separating serum from blood collected as a sample, wherein an inlet for sucking the blood by a capillary phenomenon and the serum of the blood sucked from the inlet are passed through. A blood separation device comprising: a passage portion having a gap; an air hole provided at an end of the passage portion; and a serum dropping hole provided between the passage portion and the air hole. 2. The blood separation apparatus according to claim 1, wherein a test tube for collecting the serum dropped from the serum dropping hole is detachably fixed to the position of the serum dropping hole. 3. A blood separation device for separating blood collected as a sample into blood cells and serum, which is capable of holding an inlet for sucking the blood by capillary action and the blood introduced from the inlet. A first translucent inspection window having a gap, a passage portion having a gap for passing the serum of the blood introduced from the first translucent inspection window, and the serum introduced from the passage portion A blood separating apparatus comprising: a second light-transmissive inspection window having a space capable of holding the air; and an air hole communicating with the second light-transmissive inspection window. 4. The blood separation apparatus according to claim 1, wherein the gap between the passage portions is formed in a range of 0.5 to 20 μm. 5. The blood separation apparatus according to claim 1, wherein the gap between the passage portions is formed within a range of 1 to 5 μm. 6. The blood separation apparatus according to claim 1, wherein the gap between the passage portions is formed in a range of 0.5 to 0.9 μm.