JPH07258010A - Germ-removing composition and method for removing germ - Google Patents

Abstract

PURPOSE:To obtain the composition containing a volatile germ-removing ingredient and capable of exhibiting effective germ-removing effect in a minimal amount required without requiring containment of the whole object in a closed space. CONSTITUTION:This germ-removing composition comprises a volatile germ- removing ingredient such as an isothiocyanate maximally presenting its germ- removing effect in a vapor phase or its isomer, a thiocyanate, a foaming ingredient composed of an organic acid and solid carbonate and/or bicarbonate stable at normal temperatures and pressures, and a surfactant for holding for a specified time the germ-removing ingredient vaporized into foams produced by the gas produced by reaction of the foaming ingredient.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a disinfecting composition containing a volatile disinfecting component, which is required in a minimum amount even for a relatively large article which is difficult to be entirely enclosed in a closed space. The present invention relates to a sterilizing composition capable of exhibiting an effective sterilizing effect, and a sterilizing method capable of easily performing sterilizing treatment using the sterilizing component.

[0002]

2. Description of the Related Art Generally, water around a kitchen or a bathroom is in an environment where various bacteria are easily proliferated. Also, it is not hygienically preferable that the propagation of various bacteria is left as it is. In particular, eating foods that are contaminated with harmful bacteria can cause food poisoning, so it is necessary to maintain a hygienic condition in the kitchen where foods are handled, including cooking utensils. .

Conventionally, various bacteria-removing agents have been used to remove germs, but most of the generally-used bacteria-removing agents are chlorine-based, which are harmful to the human body. There was also an accident such as death due to poisoning due to chlorine, so when using a chlorine-based disinfectant, care must be taken in handling such as ventilation, etc. There is also a problem that it is not convenient because the disinfectant needs to be thoroughly washed off after the disinfectant treatment so that the disinfectant does not remain on the cooking utensils or tableware.

On the other hand, in recent years, the antibacterial effect of isothiocyanates contained as so-called spicy ingredients on horseradish, mustard, radish, etc. has been noted, and a disinfectant containing this as a main component has been proposed. ing. Such a disinfectant is
Since it uses ingredients contained in foods that have been eaten by people such as wasabi and radish for a long time, it is highly safe and special precautions such as when using the chlorine-based disinfectant as described above. There is an advantage that the disinfection treatment can be performed without paying.

[0005]

However, since isothiocyanates exert their maximum effects when they are volatilized and in the gas phase, the isothiocyanates can be effectively sterilized. , It is necessary to bring the gas-phase isothiocyanates into contact with the object to be sterilized in a space that is hermetically sealed to some extent, which is easy and convenient. Was difficult. If it is difficult to contain the target in such a closed space, a large amount of disinfectant must be used to increase the effective concentration of the disinfecting component on the treated surface of the disinfecting target. There was also the problem of not becoming.

The present invention has been made in view of the above points, and is a sterilizing composition containing volatile sterilizing components such as isothiocyanates and thiocyanates having the same sterilizing effect. , A sterilizing composition capable of exhibiting an effective sterilizing effect in a necessary minimum amount without enclosing the entire article to be sterilized in an enclosed space, and easily using the sterilizing component It is an object of the present invention to provide a disinfection method capable of performing disinfection treatment.

[0007]

That is, the disinfectant composition of the present invention is a disinfectant composition containing a volatile disinfectant component, and contains at least a foaming component and a surfactant together with the disinfectant component. It is characterized by being

As the volatile sterilizing component in the sterilizing composition of the present invention, for example, isothiocyanates and their isomers, thiocyanates, which exhibit the maximum sterilizing effect in the gas phase, are used. be able to.

In the present invention, isothiocyanates include allyl isothiocyanate, trans-4-methylthio-3-butenyl isothiocyanate, ethyl isothiocyanate, butyl isothiocyanate, sec-butyl isothiocyanate, 3-butenyl isothiocyanate, 4-pentenyl isothiocyanate, n-hexyl isothiocyanate, phenyl isothiocyanate, benzyl isothiocyanate, β-phenylethyl isothiocyanate and the like, and as thiocyanates, 3
-Butenyl thiocyanate, 4-pentenyl thiocyanate, isobornyl thiocyanate, etc. can be exemplified, but considering the effects on the human body, allyl isothiocyanate, which is a spicy ingredient of horseradish and mustard, and trans which is a spicy ingredient of radish. -4-Methylthio-3-butenyl isothiocyanate is particularly preferred. In the present invention, these disinfecting components may be used alone, or two or more kinds selected arbitrarily from isothiocyanates and / or thiocyanates may be mixed and used.

Since the above-mentioned isothiocyanates and thiocyanates which can be used as a sterilizing component in the present invention are unstable compounds which are easily decomposed,
Prior to using the disinfectant composition of the present invention, isothiocyanates and thiocyanates as disinfectant components are decomposed, so that the disinfectant effect is not impaired, these substances are It is preferable that the inclusion compound is prepared by inclusion in cyclodextrin, and the inclusion compound is added with water or the like and placed under high humidity conditions to release the isolated isothiocyanates and / or thiocyanates. However, at this time, the isothiocyanates and / or thiocyanates volatilize to exert a sterilizing effect, and therefore, isothiocyanates and / or
Alternatively, if thiocyanates are included in cyclodextrin, it is possible to retain the sterilized component until just before using the sterilized composition of the present invention so as not to impair the sterilized effect of the sterilized component. At the same time, the time can be controlled so that the disinfecting effect is exhibited when the disinfecting composition of the present invention is used.

Further, in the present invention, the isothiocyanates and / or thiocyanates may be microencapsulated without being included in cyclodextrin. In this case, liquid isothiocyanates and / or thiocyanates are used as core substances, and polyvinyl alcohol, starch, etc. are used so that the capsules can be dissolved under high humidity conditions to volatilize the isothiocyanates and / or thiocyanates. Interfacial polymerization method using water-soluble material,
It is preferable to carry out microencapsulation by an appropriate means such as a liquid hardening coating method, a phase separation method, an interfacial precipitation method and the like.

In the present invention, the stability of the sterilized component can be improved by encapsulating the unstable sterilized component such as isothiocyanates and / or thiocyanates in cyclodextrin or microencapsulating it as described above. The sterilizing composition of the present invention can be improved, and the sterilizing component is gradually volatilized from the inclusion compound or the microcapsules placed under high humidity conditions, and the sterilizing effect is exhibited accordingly. The release effect of the disinfectant component during the use of the product is achieved, and the disinfectant effect is continuously exhibited until at least all the inclusion compounds or microcapsules in the disinfectant composition are placed under high humidity conditions. be able to.

Further, as the foaming component used in the present invention, the reaction is initiated by water or the like added to volatilize the disinfecting component as described above (that is, under high humidity conditions), and the reaction is performed at room temperature and normal pressure. It is not particularly limited as long as it generates a gas, but a solid carbonate and / or hydrogen carbonate stable at room temperature and atmospheric pressure, and an organic acid are used because of their simplicity, the generated gas and the safety of itself. Those consisting of are preferred.

Examples of the above-mentioned carbonates include sodium carbonate, potassium carbonate, magnesium carbonate, calcium carbonate, potassium magnesium carbonate, sodium potassium carbonate and the like. Examples of the hydrogen carbonate include sodium hydrogen carbonate, potassium hydrogen carbonate, magnesium hydrogen carbonate, calcium hydrogen carbonate, magnesium potassium hydrogen carbonate and the like. Furthermore, as the organic acid,
For example, malic acid, fumaric acid, succinic acid, sorbic acid,
Examples include tartaric acid, citric acid, sulfamic acid, sulfanilic acid and the like. Further, each of these carbonates, hydrogen carbonates and organic acids can be used alone or in combination of two or more.

When the above-mentioned carbonate and / or hydrogen carbonate and the organic acid are used as the foaming component in the present invention, the molar ratio of the organic acid to the carbonate and / or hydrogen carbonate is carbonate and / or hydrogen carbonate. 30 to 300 mol, preferably 10 to 100 mol of hydrogen carbonate.
The amount is 0 to 250 mol, more preferably 150 to 200 mol. When the ratio of the organic acid to the carbonate and / or hydrogen carbonate deviates from such a range, there is a disadvantage that the foaming power becomes weak.

On the other hand, as the surfactant in the present invention, a foam is formed by the gas generated by the reaction of the foaming component, and the gas generated from the foaming component and the volatilized sterilizing component are contained in the foam for a certain period of time. It is not particularly limited as long as it can be retained, for example, higher fatty acid alkali salt, alkyl sulfate, alkyl sulfonate,
Anionic surfactants such as alkylallyl sulfonates and sulfosuccinates, higher amine halogen salts, cationic surfactants such as alkylpyridinium halides and quaternary ammonium salts, polyethylene glycol alkyl ethers, polyethylene glycols Examples thereof include nonionic surfactants such as fatty acid esters, sorbitan fatty acid esters, and fatty acid monoglycerides, amphoteric surfactants such as amino acids. These may be used alone or in combination of two or more.

What is required of these surfactants is the foaming power and the stability of the foam formed, and the above-mentioned surfactants used in the present invention depend on the sterilizing component and the foaming component. However, if the amount is too small, it becomes difficult to form a foam stably, and it is possible to sufficiently retain the gas generated by the reaction of the foaming component and the volatilized sterilizing component. Can not. On the other hand, if the amount of the surfactant is too large, foaming will be worse and cleaning after the disinfection process will be troublesome. Therefore, the ratio of the surfactant to the total amount of the disinfecting composition of the present invention is preferably 0.1 to 5% by weight, more preferably 1 to 3% by weight.

In the present invention, if necessary, an extender such as sodium sulfate may be added to the sterilizing composition of the present invention within a range that does not affect the sterilizing action of the present invention, or the sterilizing composition may be treated with moisture or the like. An antiblocking agent may be added so that the invention disinfectant composition does not become lumpy.

In the disinfecting composition of the present invention, the disinfecting components, foaming components, surfactants and the like as described above are appropriately selected, processed into, for example, a powder, and these are mixed in predetermined amounts. However, in the present invention, a foaming component and a surfactant for forming a foam holding the volatilized sterilizing component are contained in the composition together with a volatile sterilizing component. What you did is especially important.

Therefore, according to the disinfectant composition of the present invention, foam is formed only by placing the disinfectant composition under high humidity conditions,
Moreover, since the volatilized sterilizing component is retained in the foam, the sterilizing component can be sufficiently brought into contact with the article to be sterilized. Therefore, even if the entire object is not contained in a closed space, the effect of the present invention can be obtained by spraying the disinfecting composition of the present invention on an object to be disinfected and adding water or the like to the effect in the gas phase. It is possible to efficiently obtain the bactericidal effect of the volatile bactericidal component that is maximized.

Next, the sterilization method of the present invention will be described.

The sterilization method of the present invention comprises a sterilization component obtained by including or microencapsulating isothiocyanates and / or thiocyanates in cyclodextrin, a foam comprising an organic acid and a carbonate and / or hydrogen carbonate. component,
And a disinfecting composition containing at least a surfactant, and after being sprayed on the disinfection object, the foaming component is reacted under high humidity conditions to form a foam, and the isothiocyanates volatilized in the foam. And / or thiocyanates are retained, and the isothiocyanates and / or thiocyanates are brought into contact with an object of disinfection treatment to perform disinfection treatment.

The above-mentioned disinfectant composition in the method of the present invention is specifically one having the composition as described above. In carrying out the method of the present invention, such disinfectant composition is first subjected to disinfection treatment. Disperse as evenly as possible on the article to be treated.

Next, water or the like is added to the disinfecting composition sprayed on the disinfecting object to react the foaming component in the disinfecting composition to form a foam on the object, The isothiocyanates and / or thiocyanates are released from the cyclodextrin or microcapsules. When the object is wet, it is not necessary to add water or the like.

Then, in the method of the present invention, the volatilized isothiocyanates and / or thiocyanates are retained in the foam formed on the object to be disinfected, and the isothiocyanates and / or thiocyanates in the gas phase are concerned. The disinfection treatment is performed by contacting the disinfection object.

According to the method of the present invention, it is not necessary to form a closed space for containing the entire object in performing the sterilization treatment with the volatile sterilizing component that maximizes the sterilizing effect in the gas phase. Not particularly, after spraying the disinfectant composition to the disinfectant treatment object, simply add water or the like to the sprayed disinfectant composition, and put the composition under high-humidity conditions to disinfect. The sterilization process of the object can be performed efficiently and easily.

[0027]

EXAMPLES The present invention will be described in more detail with reference to specific examples of the present invention.

Example 1 The disinfecting composition of the present invention was prepared with the following composition.

(Disinfecting component) Allyl isothiocyanate-cyclodextrin inclusion compound: 3 wt% (foaming component) Sodium carbonate: 30 wt% Malic acid: 40 wt% ( Surfactant) Persoft SK (manufactured by Nippon Oil & Fats Co., Ltd.) 2% by weight (Expanding agent) Sodium sulfate 25% by weight

[Comparative Example 1] In order to confirm the effect of including a surfactant in the disinfecting composition of the present invention, only the surfactant was removed from the above-mentioned Example. A disinfectant composition was prepared with the same composition as the disinfectant composition of 1.

The following tests 1 and 2 were conducted on the above-mentioned Example 1 and Comparative Example 1. The total amount of the disinfecting composition of Comparative Example 1 used in each test was made equal to the total amount of the disinfecting composition of Example 1 used in the test except for the surfactant.

[Test 1] 1 g of the disinfecting composition having the above composition was sprayed on the bottom surface of a glass container having a volume of 450 ml, and 2
A change in the concentration of allyl isothiocyanate (AITC) in the gas phase in the container with the passage of time when the container was closed after injecting ml of water was measured using a gas chromatograph.

Also, with respect to the disinfectant composition of Comparative Example 1, the change in the concentration of allyl isothiocyanate in the gas phase in the container over time was measured in the same manner as in Test 1 above.

The results are shown in Table 1 and FIG.
Incidentally, in the figure, the graph indicated by ◯ is for the disinfecting composition of Example 1, and the graph indicated by Δ is for Comparative Example 1.
Of the disinfectant composition.

[0035]

[Table 1] * However, the upper column of the AITC concentration in the gas phase is the measured value for the sterilized composition of Example 1, and the lower column () is the measured value for the sterilized composition of Comparative Example 1.

From the results of Test 1 above, the disinfectant composition of Example 1 containing a surfactant volatilizes in the gas phase more than that of Comparative Example 1 containing no surfactant. It can be seen that the amount of thiocyanate is low. If the amount of disinfectant components in the composition is the same, the amount of volatile allyl isothiocyanate should be constant regardless of the presence or absence of a surfactant, so it is better to include a surfactant. The small amount of allyl isothiocyanate that volatilizes into the phase means that in this case the volatilized allyl isothiocyanate does not diffuse into the container but is retained in the foam formed by the surfactant. Can be easily understood. Therefore, in the disinfecting composition of the present invention, by containing a surfactant in the disinfecting composition, the volatilized allyl isothiocyanate is retained in the foam formed by the surfactant, and allyl on the disinfecting treated surface is retained. Since the isothiocyanate concentration can be kept high for a longer period of time, it becomes possible to efficiently bring the sterilizing effect by sufficiently contacting the sterilized surface with allyl isothiocyanate in the gas phase.

[Test 2] A cutting board made of polypropylene (length × width; 200 mm × 400) for 15 minutes at a temperature of 120 ° C.
mm) was subjected to a steam sterilization treatment, and then the surface of the cutting board was coated with the natto bacterium suspension and air-dried to prepare a test sample. Next, the disinfecting composition 1 of Example 1 was applied to the surface of the cutting board.
0 g was sprayed as uniformly as possible, and water was added to this to foam. After a lapse of a certain time from the start of foaming, the surface of the cutting board was washed with water to remove bubbles, and the bacteria remaining on the surface of the cutting board were collected using a stamp agar medium. Then, the collected bacteria were cultured for 24 hours at a temperature of 25 ° C., and the number of colonies on the medium was measured. The relationship between the time from the start of foaming to the removal of foam (treatment time) and the number of bacteria remaining on the cutting board surface is shown in FIG. The number of bacteria remaining on the surface of the cutting board was calculated by converting the number of colonies on the medium from the ratio of the area of the culture medium to the area of the cutting board.

Furthermore, the same test as in Test 2 was conducted on the sterilized composition of Comparative Example 1 to find the relationship between the treatment time and the number of bacteria remaining on the surface of the cutting board. The results are also shown in FIG.
It was shown to. Incidentally, in the figure, the graph indicated by ◯ is for the sterilized composition of Example 1, and the graph indicated by Δ is for the sterilized composition of Comparative Example 1.

In each case, the number of bacteria remaining on the cutting board decreased with the treatment time, but in Comparative Example 1 containing no surfactant, the treatment was carried out for 5 minutes. After that, the bacteria remained on the cutting board and could not be completely eradicated. On the other hand, in Example 1 containing the surfactant, it was confirmed that the bacteria were completely removed after the treatment for 5 minutes. Moreover, it can be read from the graph of FIG. 2 that the sterilization effect of Example 1 is superior to that of Comparative Example 1 even if the treatment time is the same.

In the disinfectant composition of the present invention, the disinfectant composition having the following composition was prepared in order to confirm the effect of forming the foam holding the disinfectant component by the gas generated by the reaction of the foaming agent. It was prepared and Test 3 was performed.

[Example 2] (Bactericidal component) Allylisothiocyanate-cyclodextrin inclusion compound: 2.4 wt% (foaming component) Sodium carbonate: 23.8 wt% Malic acid ...・ ・ ・ 23.8% by weight (surfactant) Persoft SK (manufactured by NOF CORPORATION) ・ ・ ・ 2.4% by weight (filler) Sodium sulfate ・ ・ ・ ・ ・ 47.6% by weight

Comparative Example 2 A disinfecting composition having the same composition as the disinfecting composition of Example 2 except that the foaming component was removed was prepared.

[Test 3] 4.2 g of the disinfectant composition of Example 2
Was placed in a 200 ml graduated cylinder (internal diameter of about 3.1 cm), and 20 ml of water was further added to react the foaming component to form a foam, and the change in height of the formed foam was read on the scale of the graduated cylinder. . On the other hand, the sterilizing composition of Comparative Example 2 was used in the same graduated cylinder as the sterilizing composition of Comparative Example 2 in an amount equal to the total amount of the sterilizing composition of Example 2 except the foaming component. Take the
After adding 20 ml of water to this, the height of the foam is 150 m.
Air was blown with a straw until the scale of 1 was reached, and the change in foam height was read on the scale of the graduated cylinder. The results are shown in Table 1 and FIG. In the figure, the graph indicated by ◯ is for the sterilized composition of Example 2, and the graph indicated by Δ is for the sterilized composition of Comparative Example 2.

[0044]

[Table 2]

From the results of Test 3 above, when foam was formed by the reaction of the foaming agent, after the foam formation reached the peak, the foam formed gradually decreased, while When foam is formed by blowing air from the outside without depending on the agent, it can be seen that the foam formed is suddenly reduced and is not persistent. If the foam that retains the antibacterial component is formed by the gas generated by the reaction of the foaming agent, the foam formation becomes stable and stable, and the volatilized disinfectant component is continuously stored in the foam for a certain period of time. You can keep it.

Further, the following tests 4 to 6 were conducted in order to confirm the disinfecting effect of the disinfecting composition of the present invention against various fungi.

[Test 4] Commercially available Drigals
Ki agar medium (radius 8 cm) was coated with the Escherichia coli suspension and then air-dried to prepare 0.3 g of the disinfecting composition of Example 1 and one without any treatment. In the case where 2 ml of physiological saline was injected and the disinfectant composition was sprayed, the disinfectant composition was foamed and then cultured at 37 ° C. for 15 hours.

Observation of each medium after culturing revealed that the physiological saline solution on the agar of the sprayed composition of Example 1 remained clear. On the other hand, in the case of injecting only the physiological saline, the Escherichia coli proliferated remarkably and the physiological saline on the agar was extremely cloudy. As a result, it was found that the disinfecting composition of the present invention can exert an effective disinfecting effect on Escherichia coli.

[Test 5] A commercially available TCBS agar medium (radius 8 cm) was coated with a suspension of Vibrio parahaemolyticus and then naturally dried, and 0.3 g of the disinfecting composition of Example 1 was sprayed, For those prepared by injecting 2 ml of physiological saline into each of these and spraying the disinfectant composition, the disinfectant composition is foamed, and then at 37 ° C. Incubated for hours.

Observation of each medium after culturing revealed that the physiological saline on the agar of the sprayed composition of Example 1 remained clear. On the other hand, in the case of injecting only the physiological saline, the vibrio parahaemolyticus proliferated remarkably, and the physiological saline on the agar was extremely cloudy. As a result, it was found that the disinfecting composition of the present invention can exert an effective disinfecting effect on Vibrio parahaemolyticus.

[Test 6] Mannito commercially available
l Salt agar medium (radius 8 cm) was coated with a Staphylococcus aureus suspension and then air-dried to prepare 0.3 g of the disinfecting composition of Example 1 and one that did nothing. 2 ml of physiological saline was injected into each of these to spray the disinfectant composition, and the disinfectant composition was foamed, and then cultured at 37 ° C. for 15 hours.

Observation of each medium after culturing revealed that the physiological saline solution on the agar of the sprayed composition of Example 1 remained clear. On the other hand, in the case of injecting only physiological saline, Staphylococcus aureus propagated, and the physiological saline on agar was in a slightly cloudy state. As a result,
It has been found that the disinfectant composition of the present invention can exert an effective disinfecting effect against Staphylococcus aureus.

[0053]

As described above, according to the disinfectant composition of the present invention, foam is formed only by placing the disinfectant composition under a high humidity condition, and the disinfectant volatilized in the foam. Since the components are retained, the disinfecting component can be sufficiently brought into contact with the article to be disinfected, and the disinfecting composition of the present invention can be disinfected even if the entire target is not enclosed in a closed space. It is possible to efficiently obtain the disinfecting effect of the volatile disinfecting component, which exhibits its maximum effect in the gas phase, by simply spraying it on the target object and adding water or the like thereto.

In the present invention, when unstable substances such as isothiocyanates and / or thiocyanates are used as sterilizing components, these substances should be included in cyclodextrin or microencapsulated. For example, the stability of the sterilized component can be improved, and the sterilized component is gradually volatilized from the inclusion compound or the microcapsules placed under high humidity conditions, and the sterilizing effect is exhibited accordingly. As described above, the sterilizing composition of the present invention is used for sustained release, and at least until all the inclusion compounds or microcapsules in the sterilizing composition are placed under high humidity conditions. The sterilizing effect can be exerted.

Furthermore, according to the method of the present invention, when the sterilizing treatment is carried out by the volatile sterilizing component which maximizes the sterilizing effect in the gas phase, a closed space for containing the whole object is formed. There is no particular need, after spraying the disinfectant composition to the disinfectant treatment object, simply by adding water or the like to the dispersed disinfectant composition, just put the composition under high humidity conditions, It is possible to efficiently and easily perform the disinfection treatment of the disinfection object.

[Brief description of drawings]

FIG. 1 is a graph showing the results of test 1.

FIG. 2 is a graph showing the results of test 2.

FIG. 3 is a graph showing the results of test 3.

Claims (5)

[Claims] 1. A disinfectant composition containing a volatile disinfectant component, wherein the disinfectant component contains at least a foaming component and a surfactant. 2. The disinfectant composition according to claim 1, wherein the volatile disinfectant components are isothiocyanates and / or thiocyanates. 3. The disinfectant composition according to claim 2, wherein isothiocyanates and / or thiocyanates are included in cyclodextrin or are microencapsulated. 4. The foaming component is carbonate and / or hydrogen carbonate,
The disinfectant composition according to claim 1, 2 or 3, which comprises an organic acid and an organic acid. 5. A disinfecting component obtained by encapsulating or microencapsulating isothiocyanates and / or thiocyanates in cyclodextrin, a foaming component composed of a carbonate and / or hydrogen carbonate, and an organic acid, and a surface activity. A disinfecting composition containing at least an agent, after being sprayed on the disinfecting treatment object to react foaming components under high humidity conditions to form a foam, and the isothiocyanates and / or volatilized in the foam A sterilization method comprising holding a thiocyanate and bringing the isothiocyanates and / or thiocyanates into contact with an object to be sterilized.