JPH06118077A - Amine detecting method - Google Patents

Abstract

PURPOSE:To objectively detect amine in an evaporated sample with the naked eyes by gas phase color reaction by adding an alkali solution to the sample. CONSTITUTION:A vaginal secretion collected by aseptically washing the vagina with sterilized physiological saline solution is put in a vessel such as a test tube, and an alkali solution is added thereto. A solid phase (filter) containing an amine reaction coloring material such as quinhydrone or a pH test reaction solid phase (pH test paper) is sealed and suspended to confirm amines from its tone change. These may be suspended independently, but the precision of amine detection is improved by the combined use of the both. The detected amines include primary-ternary amines, and, rarely, quaternary amines. The tones in their reactions with the filters containing quinhydrone are violet, red, yellow orange, and green yellow, respectively. Thus, inspection can be objectively performed with the naked eyes.

Description

Detailed Description of the Invention

The present invention relates to an amine detection method using a gas phase color reaction which can be visually and objectively examined.

[0002]

2. Description of the Related Art In recent years, in the field of obstetrics and gynecology, vaginal trichomoniasis and vaginal candidiasis have been denied among patients who have a chief complaint of obita, and bacterial vaginosis with little local inflammation is attracting attention. .

This vaginosis often begins in relation to persistent uterine bleeding, parturition, gynecological surgery, use of intrauterine device.

[0004] Bacterial vaginosis has a complaint of a zonal sensation as a subjective symptom, and since it is not accompanied by an inflammatory symptom, a case is overlooked or neglected.

However, the putrid odor causes discomfort to a third party, and since the course of the disease is chronic, it impairs daily life, which is a serious problem for patients.

[0006] As a therapeutic method, oral administration of metnidazole and tinidazole is effective. Other local treatments using chloramphenicol improve the subjective symptoms but the therapeutic effect is not clear.

In any case, it is necessary to carry out an examination early and to give an appropriate treatment before the progress becomes heavy.

[0008] Initially, GARDNER frequently isolated Gardnerina vaginarisis from the pelvic band from patients with vaginitis whose chief complaint was the increase in bandage, and because it was not separated from healthy people, the relationship between vaginitis and Gardnerina vaginarisis was strongly asserted. It was

[0009] After that, Gardneria vaginarisis also exists in the vagina of healthy mature women, and in the vaginal secretions, other anaerobic bacteria such as Bacteroides, Peptostreptococcus and mycoplasma are present in a considerable number. I found out.

Recently, Gardneria vaginarisis is merely a marker, and anaerobic bacteria such as Bacteroides and Peptostreptococcus have come to be considered to be causative bacteria.

However, it is considered that not only anaerobic bacteria such as Bacteroides, Peptostreptococcus, and Mobilungkas, but also Gardnerira vaginarisis and other aerobic bacteria, cause this pathological condition. Has been.

[0012] These bacterial vaginosis have been diagnosed with emphasis on the examination of the characteristics of vaginal secretion. The vaginal discharge of the patient is a grayish white cream that covers the outside of the cervix to the vaginal wall and vaginal ostium in a thin film form, and a small amount of 10-20% KOH is added thereto to enhance the putrid odor.

As a diagnostic criterion for bacterial vaginosis, under the gray belt,
Vaginal pH 5 or higher, amine odor detection, Blue C
ell detection (more than 20% of endothelial cells)
Three out of four positives are diagnosed as bacterial vaginosis.

[0014]

[Problems to be Solved by the Invention] In order to rapidly test bacterial vaginosis, the above-mentioned test for confirming the putrid odor of vaginal secretions is effective for daily tests.

This is a small collection of vaginal secretions of 10 to 20
It is an amine odor test for smelling putrefactive odor that is enhanced by the dropwise addition of% KOH.

Naturally, there are individual differences in the detection of amine odors, and the odor sensation changes depending on the physical condition, and the odor sensation paralyzes as many samples are processed, and it is extremely difficult to obtain reproducible data. Therefore, the reliability is poor.

The present invention was invented in view of the above-mentioned problems of the prior art, and an amine using a gas phase color reaction which can be visually and objectively examined not by the unreliable amine odor. The purpose is to provide a detection method.

[0018]

In order to solve the above problems, in the amine detection method of the present invention, an amine in a sample volatilized by adding an alkaline solution to the sample is detected by a gas phase color reaction. To do.

The gas phase color reaction is a color reaction with an amine reaction color substance such as quinhydrone and / or a pH indicator reaction substance. The amine detection method of the present invention is suitably used for a bacterial vaginosis test.

More specifically, according to the present invention, the vaginal secretions obtained by aseptically washing the vagina with sterile physiological saline are placed in a container such as a tester, an alkaline solution is added, and then quinhydrone or the like is added. Solid phase containing the amine-reactive coloring substance (filter paper, etc.)
And / or the pH test reaction solid phase (pH test paper or the like) is respectively enclosed and suspended, and amines are confirmed from the change in color tone.

A solid phase (filter paper or the like) containing an amine reaction coloring substance such as quinhydrone or a pH test reaction solid phase (pH test paper or the like) may be hung alone in a reaction container, but both may be used together. The higher the accuracy of amine detection, the better.

Examples of the detection amines include primary amines, secondary amines, tertiary amines, and rarely quaternary amines.

The color tone in the reaction with these quinhydrone-containing filter papers is as follows: the primary amine is purple, the secondary amine is red,
Tertiary amines develop yellow-orange, and rarely quaternary amines develop green-yellow.

As the amine-reactive coloring substance, any substance that reacts with amines to develop a color can be used, but quinhydrone is preferred.

As the pH indicator reaction product, thymol blue, cresol red or the like is used. The pH indicator reaction product is not limited to these, as long as it can capture the alkalinity of amines.

When thymol blue is used, it changes to yellow due to the presence of amines, and with cresol red, the color tone changes from yellow to purple.

The present invention is not limited to vaginal secretions of bacterial vaginosis, but can be applied to any sample that generates amine.

[0028]

EXAMPLES The present invention will be described in more detail with reference to the following examples.

Example 1 A small amount of vaginal secretions obtained by aseptically washing the vagina with sterile physiological saline was placed in a 10 cm test tube, 0.5 ml of 5N NaOH saturated with sodium chloride was added, and immediately 1 was added.
The filter paper containing% quinhydrone and the thymol blue pH test paper are each placed in a hanging suspension, and amines are confirmed from the change in the color tone. The results are shown in Table 1.

Comparative Example (Conventional Method) A small amount of vaginal secretions obtained by aseptically washing the inside of the vagina with sterile physiological saline is placed on a slide, and 10% KOH is applied to the slide to smell the amine odor. The results are shown in Table 1.

[0031]

[Table 1]

Note) The evaluation evaluations in Table 1 are as follows. Amine odor: ++ Strongly + + Yes-No Quinhydrone reaction: ++ Strongly positive (strong change from yellow to red) + Positive (yellow to red change) -Negative (yellow) Thymol blue reaction: ++ Strongly positive (yellow to blue) Strong change) + Positive (change from yellow to blue) -Negative (yellow)

From the results shown in Table 1, it was confirmed that the amine detection method according to the present invention has a good correlation with the conventional method (amine odor) and the operation is simple.

[0034]

As described above, according to the present invention, an amine is used by a gas-phase color reaction that can be visually and objectively examined, not by the unreliable amine odor that must rely on the sense of smell. Is detected, it is possible to perform a large-scale inspection quickly and easily.

Claims (4)

[Claims] 1. An amine detection method, characterized in that an amine in a sample volatilized by adding an alkaline solution to the sample is detected by a gas phase color reaction. 2. The method according to claim 1, wherein the gas phase color reaction is a color reaction with an amine reaction color substance and / or a pH indicator reaction substance. 3. The method according to claim 2, wherein the amine-reactive coloring substance is quinhydrone. 4. A method for testing bacterial vaginosis, which comprises using the method according to claim 1, 2, or 3.