JPH0545363A - Biochemical analysis film container box - Google Patents

Abstract

PURPOSE:To securely house a strip-like biochemical analysis film and efficiently supply it to a biochemical analyzer so as to simplify a mechanism of the analyzer and also simply recover the used biochemical analysis film. CONSTITUTION:A biochemical analysis film container box comprises a flat box body 25 which houses an unused or used strip-like biochemical analysis film 3 folded from an opening 25a at one end and engaging protrusions 26 for holding the housing of the biochemical analysis film 3 at both ends of the opening 25a of the box body 25.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an optical system in which a sample solution such as blood or urine is spotted on a reagent layer of a biochemical analysis element and a predetermined biochemical substance contained in the sample solution and a reagent layer chemically react with each other. The present invention relates to a storage box that stores a strip-like biochemical analysis film for measuring the concentration of a biochemical substance from changes in concentration.

[0002]

2. Description of the Related Art Conventionally, a dry type chemical analysis slide capable of quantitatively analyzing a specific chemical component or formed component contained in a sample liquid simply by spot-feeding small drops of the sample liquid. Has been put to practical use. In addition, in order to quantitatively analyze the chemical components in the sample solution using such a chemical analysis slide, after spotting the sample solution on the chemical analysis slide, place this in an incubator (incubator). Incubation for a predetermined time to allow color reaction (dye formation reaction), and then for measurement that includes a wavelength selected in advance by combining a predetermined biochemical substance in the sample solution with a reagent contained in the chemical analysis slide This chemical analysis slide is irradiated with irradiation light to measure its optical density, and from this optical density, a calibration curve representing the correspondence between the optical density previously determined and the substance concentration of a predetermined biochemical substance is used. A biochemical analysis device configured to determine the substance concentration of a predetermined biochemical substance in a sample solution is used.

A technique for improving the measurement efficiency by using a long test film in which chemical analysis elements are continuously arranged instead of the above chemical analysis slide is disclosed in, for example, Japanese Patent Laid-Open No. 1-134256. It is known as can be seen.

[0004]

By the way, there is provided a strip-like biochemical analysis film in which a biochemical analysis element having a reagent layer is arranged at a predetermined interval on a long light-transmitting support as described above. Regarding the use, when setting this biochemical analysis film in the biochemical analysis device and sequentially spotting the sample solution on the biochemical analysis element for measurement, the structure of the biochemical analysis device can be simplified. In addition, efficient loading of the biochemical analysis film is important for improving the measurement efficiency, and it is necessary to surely accommodate the biochemical analysis film to prevent the biochemical analysis film from popping out.

Further, in the used biochemical analysis film which has been sequentially spotted with the sample liquid on the above-mentioned biochemical analysis film in the biochemical analysis device, the biochemical analysis element is contaminated by blood or the like attached thereto. However, if a worker touches and discards it by hand, he or she may cause an infectious disease unless paying sufficient attention, resulting in a decrease in treatment efficiency.

Therefore, the present invention has been made in order to solve such a problem, and the strip-like biochemical analysis film is surely accommodated and efficiently supplied to the biochemical analysis device so that the mechanism of the device is realized. It is an object of the present invention to provide a storage box for a biochemical analysis film, which is simplified and allows the used biochemical analysis film to be easily collected.

[0007]

In order to achieve the above object, the biochemical analysis film container of the present invention comprises a biochemical analysis element having reagent layers at predetermined intervals on a long light-transmissive support. A box-shaped box portion main body for accommodating the arranged strip-like biochemical analysis film, and accommodating the biochemical analysis film by folding from the opening at one end, and an opening of the box portion main body. It is characterized in that the both ends are provided with engaging projections for holding the accommodation of the biochemical analysis film, and supply of the biochemical analysis film to the biochemical analysis device and recovery of the biochemical analysis film after use are performed. It is a thing.

Further, it is preferable that the engaging projection is formed of an elastic member.

[0009]

In the storage box for biochemical analysis film as described above, the strip-shaped biochemical analysis film is folded and stored in the box-shaped box body from the opening at one end, and the stored biochemical analysis film is stored. The chemical analysis film is held so as to prevent the projection of the chemical analysis film by the engaging projections, and the cartridge-shaped biochemical analysis film can be easily loaded into the biochemical analysis device.
A simplification of the mechanism is obtained.

Similarly, a used biochemical analysis film is similarly folded and accommodated in the box-shaped box main body from the opening at one end, and when the biochemical analysis film is collected, the biochemical analysis film is folded. It has a function to guide it so that it can be easily removed, and recovery work can be performed without directly touching the biochemical analysis film after use. Furthermore, the supply and recovery of the biochemical analysis film can be performed at the same time, and the amount of waste can be reduced.

[0011]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing a biochemical analysis device in which a biochemical analysis film accommodating box of one embodiment is loaded, with a part thereof being cut away.

In the biochemical analyzer 1, a sample liquid storage means 4 for storing a sample liquid such as serum and urine in a circularly arranged storage portion 40 is installed at one end of the main body and is stored therein. Each of the sample liquids W thus rotated is sequentially rotated to the extraction position by rotation, and is extracted by the spotting means 5 provided on the upper part so as to be movable in the front, rear, left, and right directions, and the strip-like biochemical analysis film 3 having a configuration described later is formed. It is spotted on a predetermined biochemical analysis element 31. This biochemical analysis film 3 contains a specific chemical component to be measured in the sample liquid or a reagent that shows a color reaction with only that component for each formed component, and a plurality of types corresponding to the measurement item are prepared. It is prepared.

As shown in FIGS. 4 and 5, the biochemical analysis film 3 has a biochemical analysis element 31 arranged in a striped pattern at predetermined intervals, for example, PET.
(Polyethylene terephthalate) or the like, a long light-transmissive support 32, and a development layer laminated on the support 32.
A substantially square-shaped multilayer chip 33 composed of 33a and a reagent layer 33b, and an outer peripheral portion of each multilayer chip 33 is sandwiched between the support 32 and a space above the central portion of the multilayer chip 33. It is constituted by a light-shielding film-like covering member 34 for sealing the multilayer chip 33 in a moisture-proof state by providing the portion 34a.

The film-like cover 34 is made of a material, such as PE (polyethylene), which can be easily pierced by an injection needle-like spotting needle 51 described later. In addition, the spreading layer 33a of the multilayer chip 33 described above allows the sample solution W to
The reagent layer 33b is formed of a material that can be spread over substantially the entire surface of the sample 33. When the reagent layer 33b is mixed with a predetermined sample liquid component, a reagent that causes a color reaction (dye formation reaction) after being kept at a constant temperature (incubation) for a predetermined time is formed. Contains. Further, in the biochemical analysis film 3 described above, a perforation 35 is perforated at one side edge of the light transmissive support 32, and it is engaged with sprockets 61 and 62 of the conveying means 6 which will be described later.
Folds (not shown) such as notches are formed at predetermined intervals to facilitate folding.

The biochemical analysis film 3 is folded and stored in a storage box 11 on the supply side for each measurement item, and a plurality of the biochemical analysis films 3 are loaded in parallel in a refrigerator 13 at the front of the main body as shown in FIG. The used biochemical analysis film 3 that has been fed from the supply-side storage box 11 to the measuring unit on the incubator 8 described later by the transporting means 6 and the measurement of which has been completed is stored in the recovery-side storage box 12 arranged at the rear side. It is folded and stored in and stored. At one end of the biochemical analysis film 3, for example, a bar code indicates the lot number, individual number, measurement item, expiration date, etc. of the biochemical analysis film 3.

The supply side storage box 11 and the recovery side storage box 12
Have the same structure, and the one after the supply of the stored biochemical analysis film 3 is used as the recovery side storage box 12.
As shown in FIG. 2, the structure is such that the box body 25 is formed in a flat shape and has an opening 25a at one end thereof. Further, engaging projections 26 made of elastic members (leaf springs) are provided at both ends of the opening 25a of the box body 25.

The housing boxes 11 and 12 have openings 25.
The unused or used biochemical analysis film 3 is sequentially folded from the inside from a to be accommodated, and the biochemical analysis film 3 accommodated by the engaging projections 26 at both ends of the opening 25a is prevented from popping out.

A modified example of the biochemical analysis film accommodating boxes 11 and 12 is shown in FIG. 6, and the engaging projections 27 at both ends of the opening 25a of the box body 25 are simply formed by projections. The protrusion may be made of an elastic body.

Next, the spotting means 5 has a spotting nozzle 50 for sucking and discharging the sample liquid W, a spotting needle 51 is attached to the tip of the spotting nozzle 50, and is moved forward and backward by the moving means 9. And moved in the left-right direction, and the sample liquid W
And take it out from the supply side storage box 11 and convey means 6
Then, the biochemical analysis film 3 drawn out on the incubator 8 is spotted. Further, the moving means 9 is configured so that the spotting means 5 can be moved in the vertical direction,
When the spotting means 5 is moved in the front-rear, left-right direction by the moving means 9, the spotting means 5 is in the raised position,
When the sample liquid W is taken out and spotted, it is lowered. Further, the spotting needle 51 at the tip of the spotting nozzle 50 of the spotting means 5 is replaced with the change of the sample liquid W, and the replacement spotting needle 51 is the accommodating portion 40 of the sample liquid accommodating means 4. It is held on the inner circumference side of.

The spotted biochemical analysis film 3 is incubated in an incubator 8 and measured by a measuring means 7 arranged below the incubator 8. The control of the operation of the biochemical analyzer 1 and the processing of measurement data are performed by a built-in circuit section and a computer, and a keyboard 22 for giving an instruction, a display 23 for displaying auxiliary information for the instruction, a measurement result, etc. A printer 24 for printing out the measurement results is installed.

2 and 3 are schematic sectional views of the spotting measuring portion. The spotting means 5 is moved in the front-rear and left-right directions by the XY driving section 91 of the moving means 9 to a suction means (not shown). The connected spotting nozzle 50 is hung down by the XY drive unit 91, and a spotting needle 51 having a tip pointed like an injection needle is detachably attached to the lower end portion thereof.

The biochemical analysis film 3 delivered from the supply side storage box 11 to the incubator 8 is meshed with the sprocket 61, 62 of the conveying means 6 arranged before and after the incubator 8 in the perforation 35 thereof. At the same time, the biochemical analysis film 3 is guided by the sprocket guide 63 by a driving means (not shown), and the used portion of the biochemical analysis film 3 after the measurement is used for collecting side storage box 12 (see FIG. 2). (Not shown).

Below the exposed portion of the biochemical analysis film 3 between the supply side storage box 11 and the recovery side storage box 12, an incubator 8 whose inside is kept at a predetermined temperature (for example, 37 ° C.) is installed. ing. Further, a channel-shaped strip presser 81 is provided on the incubator 8 extending in the transport direction of the biochemical analysis film 3 and having an opening 81a through which the spotting needle 51 is inserted. Further, the incubator 8 is provided with a large number of photometric windows 82 of a predetermined size corresponding to the arrangement intervals of the chemical analysis elements 31.

A plurality of (four in the illustrated case) photometric heads for measuring the optical density due to the color reaction of the biochemical analysis film 3 and the sample solution W are provided below the incubator 8.
A measuring means 7 having 71 is arranged. The photometric head 71 is arranged in parallel with the moving frame 72, and the moving frame 72 is reciprocally moved in the left-right direction (front-back direction in FIG. 2) by a moving means (not shown) to sequentially scan the biochemical analysis element 31 after spotting. It is provided to do.

The photometric head 71 irradiates the reagent layer 33b with measurement irradiation light containing light of a predetermined wavelength, and reflects light to the photodetector element.
73, and each photometer head 71 has a single light source.
The light from the 74 (lamp) is incident on one end of the four optical fibers 77 via the lens 75 and the filter 76, and the other ends of the optical fibers 77 are respectively connected to the photometric head 71.
It is connected to the. In this photometric head 71, the light from the other end of the optical fiber 77 passes through the lens 78 and passes through the reagent layer.
It is irradiated on 33b. The filter 76 is configured such that a plurality of filters corresponding to inspection items are installed on the disc 76a, and the disc 76a is rotated by a motor 79 to select a filter having a predetermined characteristic.

The reflected light from the reagent layer 33b is reflected by the reagent layer 33.
The optical information (specifically, the amount of light) corresponding to the amount of the pigment generated in b is carried, and the reflected light carrying this optical information is incident on the photodetection element 73 and photoelectrically converted, and the amplifier 15 is turned on. It is sent to a dye determination unit (not shown) via the. In the dye determination section,
The optical density of the dye formed in the reagent layer 33b is determined based on the level of the input electric signal, and the substance concentration of a predetermined biochemical substance in the sample liquid W is specified.

In FIG. 1, reference numeral 16 denotes an electrolyte measuring unit, an electrolyte containing box 17 is installed in front of the electrolyte measuring unit, and a case 18 covers the upper part of the measuring unit of the main body and the upper part of the refrigerator 13.

The measurement by the biochemical analyzer 1 will be described. A supply side storage box 11 and a recovery side storage box 12 containing a biochemical analysis film 3 having a reagent layer 33b corresponding to a measurement item are loaded, and The biochemical analysis film 3 fed out from the supply side storage box 11 is set on the conveying means 6 and fed out to a predetermined position on the incubator 8.

Based on the measurement command, the spotting nozzle 50 is moved by the moving means 9 onto the predetermined containing portion 40 of the sample liquid containing means 4 to immerse the spotting needle 51 in the sample liquid W, and the spotting needle 51. A predetermined amount of sample liquid W is sucked into the inside. Then, the spotting nozzle 50 is moved by the moving means 9 onto the biochemical analysis element 31 of the biochemical analysis film 3 corresponding to the measurement item, and then the spotting nozzle 50 is moved downward, as shown in FIG. , A film-like cover 34 of the biochemical analysis element 31 at the tip of the spotting needle 51.
Break through. After this, from this spotting needle 51 to the multilayer chip 33
A predetermined amount of the sample liquid W is dropped on the spreading layer 33a. The dropped sample liquid W is diffused in the spreading layer 33a, leaches into the lower reagent layer 33b, and mixes with the reagent.

Subsequently, the biochemical analysis element 31 that has been spotted
When heated to a predetermined temperature by the incubator 8, the reagent layer 33b causes a color reaction (dye formation reaction). Then, after every predetermined time during the color reaction or after a predetermined time has elapsed, the optical density of the dye generated by this color reaction is measured by the photometric head 71. When the spotting of the predetermined number of measurement items is completed, the spotting needle 51 is replaced and the next sample liquid W is sucked and spotted. As described above, the sample liquid W is sequentially spotted on the biochemical analysis element 31 corresponding to the required inspection item, and the coloration state is sequentially scanned by the photometric head 71 to measure a plurality of sample liquids W and measurement items. ..

The used portion of the biochemical analysis film 3 after the measurement is sequentially folded and stored in the collecting side storage box 12, and the used biochemical analysis film 3 is spotted and contaminated with the sample solution W.
Take it out of the device 1 without touching it and dispose of it.

The spotting needle 51 of the spotting nozzle 50 is
The biochemical analysis film 3 may be spotted on the biochemical analysis film 3 and then washed by the nozzle washing unit so as to be reused for the next spotting.

[Brief description of drawings]

FIG. 1 is a schematic perspective view showing a biochemical analysis device having a biochemical analysis film accommodating box according to an embodiment of the present invention partially cut away.

FIG. 2 is a schematic cross-sectional view of a spotting measurement portion of the biochemical analysis device of FIG.

3 is a sectional view taken along the line AA of FIG.

FIG. 4 is a perspective view of a biochemical analysis film.

FIG. 5 is a cross-sectional view showing a spotted state on a biochemical analysis film.

FIG. 6 is a sectional view showing a modified example of a storage box for biochemical analysis film.

[Explanation of symbols]

 1 biochemical analysis device 3 biochemical analysis film 4 sample liquid accommodating means 5 spotting means 6 conveying means 7 measuring means 8 incubator 9 moving means 11,12 accommodating box 25 box body 25a opening 26,27 engaging protrusion W sample Liquid 31 Biochemical analysis element 32 Light-transmissive support 33b Reagent layer 33 Multilayer chip 34 Covering body 51 Pointing needle 71 Photometric head

Claims (2)

[Claims] 1. A storage box for storing a strip-shaped biochemical analysis film in which biochemical analysis elements having reagent layers are arranged at predetermined intervals on a long light-transmissive support, the storage box having one end A flat box body for folding and accommodating the biochemical analysis film through the opening, and engaging projections for holding the biochemical analysis film at both ends of the opening of the box body, A storage box for a biochemical analysis film, characterized by supplying the biochemical analysis film to a chemical analysis device and collecting the biochemical analysis film after use. 2. The storage box for a biochemical analysis film according to claim 1, wherein the engagement protrusion is formed of an elastic member.