JPH01503595A - Contamination prevention closures, especially screw closures for cell culture bottles - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] Contamination-protection closure, especially screw closure for cell culture bottles The present invention provides a contamination-protection closure. the body, especially for biological, virological, immunological and cytological analysis and diagnosis. Cultivate the texture.

Concerning screw closures for sterile cell culture bottles designed to preserve tissue cultures. 6. This kind of bottle is usually made of polystyrene.

Sterile, non-returnable containers made of plastic such as polycarbonate or polypropylene. It is offered as a Noh bottle by various manufacturers. Such a bottle is, for example, a parallelepiped. shaped, these bottles can be used in horizontal as well as vertical positions, RW & Mirror These bottles are made of clear plastic and have a neck of 0. A cylindrical or Erlenmeyer flask with narrow or wide openings. There are also bottles in this form. The neck of the bottle, which has a male thread, is tightly sealed. 0, which is equipped with a screw-type closure used for closing purposes.

The bottles are typically used for biological research, e.g. biochemical, immunological, and diagnostic. Culture cells of fresh trypsinized tissue.

Commonly used for permanent and semi-permanent culture and preservation of human, animal and plant cells. used.

As mentioned above, this type of bottle contains cell culture medium and microorganisms (filamentous fungi).

It is used for culturing and preserving bacteria, yeast, and L virus. However, In most cases, these bottles contain gases (Co2.02.N2.H2 ) or alternatively, if gas exchange between the inside and the outside is not possible, No, regarding culture techniques and biological (viruses, bacteria, fungi) measurements. 'Monoklonale AntikirparJ' of Jl-do Beater et al. See 1985.

Achieve the presence of some gas mixture. III. Gas exchange between the inside of the bottle and the atmospheric air The common way to achieve gas exchange between atmospheric air and the inside of the bottle is to The threads are formed through the labyrinth passage of the mutually threaded threaded parts of the neck and closure body. This is done by removing the hermetic bottle seal by slightly loosening the closure.

It is very often desirable to supply or exchange gas instantaneously These methods have a number of drawbacks, one of which is that replacement takes too long. Due to the fact that This form of gas exchange is performed under clean conditions in the room. Only if body exchange takes place, i.e. when the atmospheric air is essentially sterile and free of particles. Instead of acceptance.

The new type of hermetic seal is released by releasing the screw clamp. The contents of a are full. Despite the low loading level9, e.g. as a result of shaking action or during exercise and transport. Since it is possible to reach close to the opening of the bottle, biological ( Dangerous or toxic gases (e.g., immunologically) can flow out through the threads. There is a risk that the user may become infected while handling the bottle.

Similarly, of course, the cell culture medium is removed during handling of the bottle; There is a risk of contamination. Another danger is the shaking effect of the gap in the threaded part. As a result, it can be filled with concentrated water or liquid, which is almost completely disabled. It will be a body exchange.

The same applies especially to methods that are often used in practice.

With this method,9 for example, an Erlenmeyer flask can be sealed with a sterile cotton plug. will be sent.

Therefore, the object of the present invention is to provide a closed body that ensures aseptic ventilation of the bottle, especially under poor atmospheric conditions. This protection against contamination by microorganisms (bacteria, fungi) at the same time as in a manner that allows for overall maintenance without adversely affecting handling characteristics throughout the bottle. ! Modify the screw closure or cell culture medium bottle of the type described above by suitable means. There are many things.

According to the present invention, this subject matter is disclosed by means of the claims and scope paragraphs 1 or 12. will be achieved. As for other developments that are advantageous.

This is stated in the subsection.

Permanent bottle closures that are secure against cellular and microbiological contamination permanently equilibrate with atmospheric air. It ensures uniform gas exchange and the filter element is free even in the case of impure atmospheric air. Advantages are seen in the fact that it ensures that only bacterial gas can enter the bottle. Tori Handling has also been made safer, and filter elements are now preferably placed in contact with the user. Arranged inside the enclosure in a location that is inaccessible and therefore cannot be contaminated. be done.

Bottles used in a horizontal position have a shaking action that allows higher culture products to be obtained. Even if you receive it, you can fill it up to a higher level.

Concept 1 of the present invention: Details are given through numerous embodiment examples with reference to the accompanying drawings. Explain.

FIG. 1 is a cross-sectional view of a closure on layers having the shape of a parallelepiped.

FIG. 2 is an enlarged cross-sectional view of a detail of the neck of a bottle with a modified closure.

Figure 3 is an oblique view of another conventional bottle on the market.

Figure 4 shows the tilt of an Erlenmeyer flask with a screw closure or a bottle with a similar shape. View.

The 5th m shows a modified closure.

FIG. 6 is a sectional view of another modification of the screw type closure.

No. 7m is the bottom surface of the innermost part of the screw closure shown in Figure 4 without the filter element.

FIG. 8 is a plan view of the screw-type closure of FIG. 6 without the J-retaining sheath.

FIG. 9 shows a detailed sectional view of another embodiment of the screw closure with staggered holes. show.

FIG. 10 is a sectional plan view of the screw closure of FIG. 9;

According to Figure @1, bottle F is partially filled with liquid cell culture medium Z and protected against contamination. It is sealed with a screw type closure 1. The screw closure 1 according to FIGS. 1 and 2 is It has a constant female thread 7, and this female thread 7 matches the male thread of the bottle neck BF', and the bottle neck The inner shoulder 6 of section F° is firmly pressed against the upper rim of bottle neck g-11F' .

According to the present invention, 2/47 of the closure body 1 covering the floor frontage F° is made of plastic. and is provided with at least one hole 3. Figure 6 θjisei! ! ! For sickles, close Inside the chain body 1 is a drainage filter support 5 in the form of a 1° circular male support. A flat filter with hydrophobic holes is placed on the circular collar 2 degrees above which is an integral structure. The piece 4 is secured against leakage, preferably at its edges by hermetic sealing or ultrasonic welding. It's still there.

According to FIGS. 6 and 9, the holes 3 provide additional mechanical protection for the use of the filter element 4. with protection or to allow gas exchange as desired; or not; The entire body can be covered by the self-adhesive protective sheath 8. @ According to Figure 5 and Figure 1O In embodiments, accidental mechanical damage to the flat filter piece by the user Similarly, contamination due to contact with the flat filter piece 4 will result in uninterrupted holes 3. However, it is prevented.

Figures 9 and 810 show the axial and radial orientation within the wall thickness of each hole 3F stick body. It is provided with a offset 3' so that the user's flat filter piece 4 can be accessed through the hole 3. damage is actually [; indicates an impossible embodiment].

A mechanism for preventing biological contamination in the screw closure of the embodiment of FIGS. 9 and 10. Under specified conditions of use, the user cannot sample the contents of the bottle without removing the screw closure. A defined filter area other than the area of the neck opening HF of the bottle to allow the pull to be taken out. Silicon plugs 9 located outside the area are arranged, and the silicon plugs 9 shows that the plug itself is removed after the inserted hollow needle is removed after taking out the sample. It is of such a diameter and thickness that it again forms an airtight seal.

(Not shown) -Actual% In the case of Q, the filter element is a silicone plug 9. It consists of a filter body in the form of a cartridge that takes the configuration. This is also illustrated, In alternative embodiments, the cover of the screw closure itself is perforated. Takes the form of a sintered plastic part, which allows gas exchange: liquid Prevents the emission of

Implementation according to Figure 3? In all cases, there is a filter element 4 on the wall of the bottle or on the shoulder of the bottle. and a window sealed with a protective sheath 8 is located. In this embodiment, The screw closure does not include a filter element.

In all cases, the filter material also contains an inert plastic to the cell culture medium. Made of stick material 9 e.g. polypropylene, polytetrafluoroethylene, silicone made of hydrophobic cellulose derivatives, which are toxic to liquids and cell culture media. It allows gas exchange under sterile conditions without toxicity or cytotoxicity, while also It has a pore size or cut-off that inhibits the growth of cells and microorganisms.

Translation of amended sound copy a) Submission form (Article 184-8 of the Patent Law) February 23, 1999 Day

Claims (1)

[Claims] 1) Closed bodies to prevent contamination, especially biological, virological, immunological and cytological used for culturing and preserving cell and tissue culture media, as well as for analytical analysis and diagnosis A plastic screw closure for use as a sterile cell culture medium bottle. Therefore, the part (2) of the closure (1) that covers the bottle opening (F') is made of plastic. , a hydrophobic filter element (4) integrally formed therewith, - the element (4) is inert to the cell culture medium (Z) and sterilized between the bottle contents and atmospheric air; Allows gas exchange, cellular and microscopic movement from the inside to the outside and vice versa. Characterized by having a pore size or cut-off that prevents biological contamination closed body. 2) The part (2) of the closure (1) in which the filter element (4) covers the bottle opening (F') fixedly positioned against contact from the outside behind at least one hole (3) of the A closure according to claim 1, characterized in that: 3) The filter element is formed by a sintered perforated part of the plastic closure (1). A closure according to claim 1 or 2, characterized in that: 4) A cartridge filter in which the filter element is disposed within the open chain body (1). The closure according to claim 1 or 2, characterized in that it is molded. 5) at least one hole (3), preferably a number of holes (3), with the opening of the bottle to be covered; sealed and welded on the inside or outside of the closure body (1) in the area of section (F'); or the filter element (4) is formed by a microporous flat filter piece that is glued. A closure according to claim 1 or 2, characterized in that: 6) a closure body (defined by a defined edge (2') of the flat filter piece (4); 1) is characterized in that the inner region is provided with a drainage filter support (5) A closure according to claim 1 or claim 5. 7) a shaft in which the hole (3) in the thickened inner closure (1) seals the direct axial passage; directional and radial cross-sectional offset (3'), said offset being microporous. characterized in that it protects the membrane (4) against contact with the outside and against mechanical damage. A closure according to claim 1, claim 5, or claim 6. 8) The upper surface of the area of the closure (1) that covers the bottle opening (F') and has a hole inside. is flat and has a removable protective sheath (8) that covers and seals the hole (3). A closure according to any one of claims 1 to 7, characterized in that: 9) The microporous membrane has a pore size of 2 μm to 0.0001 μm. A closure according to claims 1 to 7. 10) A sealing plug (9) made of silicone etc. is defined by a filter element (4). The closure (1) outside the area of the closed closure (1) and in the part of its wall (2) said area being defined by a bottle opening (F') and said sealing plate The lug (9) is suitable for the sample taken by the needle and the opening for the needle is Claim 1 characterized in that the cross section is adapted to close on itself. and the closed body described in paragraph 9 of the same. 11) A non-woven fabric characterized by the features according to one of claims 1 to 10. A closed fixture that protects the cultured cell culture substrate from contamination. 12) Cultivate and preserve tissue culture media and perform biological, virological, immunological and Contamination prevention with cell culture substrates made of plastic for mycological analysis and diagnosis The cell culture substratum (F) is located in the region of the neck or shoulder of the bottle. The screw closure body (1) has an opening on the lower side, and the opening is an opening from the rim side. The filter is sealed by a hydrophobic filter element (4) in sealed communication. - The element (4) is inert to the cell culture medium (Z) and sterilized between the bottle contents and atmospheric air. Allows gas exchange, by cells from inside to outside and from outside to inside Shaped with filter material that has a pore size or cutoff that prevents contamination by biological organisms. A closed fixed body characterized by: