JPH01465A - Composition for detecting occult blood and test body coated with the composition - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] [Industrial application field]

The present invention relates to a composition for detecting occult blood and a test specimen, specifically,
The present invention relates to a detection composition for simply and quickly detecting blood in urine, feces, and body fluids, and a test body formed using the detection composition. K. PRIOR ART Detection of occult blood in body fluids such as urine, proboscis, gastric fluid, or fecal excrement is a very complicated means for diagnosing many pathological conditions. Conventionally, to detect white fluid in body fluids, especially urine, test specimens coated with occult blood test reagents have been mainly used. This test specimen has the advantage that it is easy to operate and can make a determination in a short time. However, in conventional test specimens for detecting occult blood, peroxide is decomposed by the peroxidase-like action of hemoglobin in the blood (an action similar to peroxidase), and the oxygen generated during decomposition reacts with the oxidizable indicator. The peroxidase-like action of autochromatic pigments in blood is weak to contribute to satisfactory sensitivity and color density in dry chemistry, so in order to solve this problem, quinoline A test piece containing a quinoline derivative and a peroxidase-like action (in other words, sensitization) has been proposed (Japanese Patent Publication No. 5680/1983). However, when the peroxidase-like action is enhanced in this way, satisfactory sensitivity and color density are obtained when using highly sensitive oxidizable indicators such as 0-tolidine and benzidine, but other oxidizable indicators When using an indicator, there is a drawback that satisfactory sensitivity and color density cannot be obtained. Furthermore, the peroxide contained in the occult blood test strip is also decomposed by contact with the indicator and buffer agent contained at the same time, resulting in a decrease in sensitivity and color density. In order to solve this problem and provide long-term storage stability, microencapsulation of organic hydroperoxides using gelatin has been proposed (Japanese Patent Publication No. 54-10520 @ Publication). However, microencapsulation requires many steps and, moreover, the microcapsule film hardens during storage, making it less likely to disintegrate quickly when in contact with water. The sensitivity and color density of occult blood test specimens that have been stored for longer than this period will decrease in actual use.In addition, if the organic peroxide and other indicators are separated by microencapsulation, Similarly, it is difficult to obtain satisfactory sensitivity and color density.Under these circumstances, the applicant first dissolves or disperses the oxidizable indicator, organic peroxide, and binder.
(Japanese Patent Application Laid-Open No. 59-3378 γ) proposes a method of manufacturing a specimen by printing an ink composition on a support. Although this method has a simplified manufacturing process and can efficiently manufacture test pieces, it is not necessarily sufficient in terms of storage stability and sensitivity.

[Problems to be solved by the invention]

The present invention improves the invention previously made by the applicant in consideration of the prior art, and provides a composition for occult blood testing and a test specimen.
It has excellent sensitivity and high color density, ■ It has excellent storage stability, ■ It can be dried at low temperatures and does not cause deterioration of the indicator, and ■ The application method, especially printing. This was done with the aim of creating a device that satisfies all the requirements that an inspection area can be formed by the method.

[Means for Solving the Problems 1] In order to solve the above problems, the present invention microcapsules either the organic hydroperoxide or the oxidizable indicator, thereby preventing the organic hydroperoxide from being released during storage. A composition for detecting occult blood that prevents it from being decomposed by reacting with other substances, has high sensitivity and color density using a sensitizer, and can be manufactured quickly by means such as printing. This is the specimen obtained. The present invention provides organic hydroperoxides, oxidizable indicators, sensitizers, pl+ that are capable of producing a detectable response in the presence of the organic human O peroxide and the peroxide active substance.
A composition for detecting occult blood in which a buffer, a binder, and a water-absorbing powder are dissolved or dispersed in a nonaqueous solvent, the components being a first group containing an organic hydroperoxide, and an oxidizable indicator. Either the first group or the second group relates to a composition for detecting occult blood that is microencapsulated with a water-soluble microcapsule coating material. In addition to the organic human O peroxide, the components included in the first group include a sensitizer and a part of the binder, and although microencapsulation may be performed on the organic hydroperoxide alone, it is preferable to Microencapsulation is performed by adding an organic hydroperoxide, a sensitizer, and, if necessary, a binder. The sensitizer will be described in detail later, but in the present invention, either quinoline or its derivative or triethanolamine salt is used, and it is particularly preferable to use both in combination. On the other hand, microencapsulation should be performed for components included in the second group, oxidizable indicators, pHai buffers, water-absorbing powders, and some of the binders that may react with organic hydroperoxides. You can also do it. Further, in the present invention, the above-mentioned composition can be placed on a support and used as a test object. namely, an organic hydroperoxide, an oxidizable indicator capable of producing a detectable response in the presence of the organic hydroperoxide and the peroxidation active substance, a sensitizer, a p11am agent 2 binder, and a water-absorbing carrier powder. A composition for detecting occult blood, in which either a first group containing an organic hydroperoxide or a second group containing an oxidizable indicator among the constituent components is microencapsulated with a water-soluble microcapsule coating material. The resulting composition is used as an ink composition and applied onto a support to prepare a test object for detecting occult blood. 1. Effect] According to the present invention, the composition for detecting occult blood is divided into a group of components containing an organic hydroperoxide and a group of components containing an oxidizable indicator, and either of them is microencapsulated. Since the hydroperoxide does not react with other substances during storage and a sensitizer is used, the sensitivity of the reaction after the capsule disintegrates can be increased. KX Example] Each component of the composition for detecting occult blood in the present invention and the production of the sample for detecting the occult surface will be explained in order below. Organic hydroperoxides (among many well-known compounds belonging to the group of hydroperoxides) can react with peroxide-active substances in the presence of peroxide-sensitive indicators to cause a color change or Any substance can be used as long as it can produce a detectable response, such as a change in light absorbed or reflected by the specimen.For use in the composition for detecting occult blood of the present invention, 111, such as cumene hydroperoxide, t-butyl hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide, diisopropylbenzene hydroperoxide, or paramenthane hydroperoxide. , or a mixture thereof. Among these, it is preferable to use cumene hydroperoxide. The organic hydroperoxide accounts for 1 to 30% by weight, preferably 2% by weight, based on the solid content of the composition for detecting occult blood. Microcapsule coating material The microcapsule coating material that can be used in the present invention contains the components constituting the composition for detecting occult blood, organic hydroperoxide, When divided into a first group consisting of a sensitizer and a part of a binder, and a second group consisting of an oxidizable indicator, a p11 buffer, a water-absorbing powder, and a part of a binder, the first group An emulsifier that forms a good emulsion when mixed with any of the components that cover f1 or any of the components that belong to the second group, and that quickly disintegrates when it comes into contact with water in the test liquid and does not encapsulate the emulsifier. It is desirable that the microcapsule coating material has the ability to involve organic hydroperoxides or oxidizable indicators, etc., in the color reaction.The following microcapsule coating materials have been confirmed to meet these conditions. Water-soluble synthetic resins such as polyvinyl alcohol resin, polyvinylpyrrolidone resin, polyacrylamide resin, or polyvinyl acetate resin: 1 water-soluble cellulose such as methylcellulose, ethylcellulose, hydroxycellulose, or carpoxymebu cellulose;
Examples include natural polymers such as starch, polysaccharides, gelatin, casein, gum arabic, and sodium alginate. Among these, gum arabic, carboxymethyl cellulose, and vinyl alcohol resin are particularly preferred. The microcapsule coating material is preferably present in an amount of 2 to 70% by weight, preferably 4 to 50% by weight, based on the solid content of the composition for detecting occult blood. Oxidizable Indicator The oxidizable indicator is an indicator that develops color when oxidized by an enzyme, and a wide variety of conventionally known compounds such as benzidines and N-alkylated benzidines can be used. Among these, o-tolidine, 3.3', 5.5'
-Tetramethylbenzidine, 2,7-azafluorene, etc. are preferred, especially 3,3', 5.5'
-Tetramethylbenzidine is preferably used. It is desirable that the oxidizable indicator be present in an amount of 0.5 to 10% by weight, preferably 0.6 to 6% by weight, based on the solid content of the composition for detecting occult blood. Sensitizer By using either quinoline or a derivative thereof and a triethanolamine salt, or a mixture thereof as a sensitizer, color formation of a specimen for detecting occult blood in the presence of white liquor is promoted, Sensitivity and? Color density can be improved. As quinoline or its derivatives, quinoline, quinine, 6-medoxyquinoline, 6-methylquinoline, or 7-methylquinoline are used, among which 6-
Particularly preferred is medoxyquinoline. As the triethanolamine salt, triethanolamine salt M1. Triethanolamine phosphate, triethanolamine sulfate, etc. are used, and among these, sulfates such as lauryl sulfate triethanolamine and polyoxylauryl sulfate triethanolamine are preferred, and especially lauryl acid triethanol Preferably, amines are used. The amount of quinoline or its derivative is 0.5 to 10% by weight, preferably 0.6 to 10% by weight based on the solid content of the composition for detecting occult blood.
Preferably, it is present in an amount of 6% by weight. Further, the triethanolamine salt is contained in an amount of 1 to 20%, preferably 2 to 15%, based on the solid content of the composition for detecting occult blood.
It is desirable that it be present in about 5% by weight. Buffers pH buffers are pH buffers at pH levels near the pH at which the oxidizable indicator described above produces a preferred ♀ color change in the composition for detecting occult blood.
[Used to maintain. The ptlfj agent may be any agent as long as it can impart a predetermined pH value (for example, pH 5) to the composition for detecting occult blood; specifically, it is preferable to use a combination of citric acid and sodium citrate. . Binding material The binding material must be one that does not affect the components and pH value of the test liquid, does not affect reagents, especially oxidizable indicators, and does not interfere with the i-color reaction. required. The following binders have been confirmed to meet these conditions, and these are used either in combination or as a mixture of two or more of them. Polyester resin, alkyd resin, 9-weight urethane resin, polystyrene resin, acrylic resin, epoxy resin, vinyl chloride resin, vinyl chloride copolymer resin, polyvinyl butyral resin, polyvinyl alcohol resin, polyvinyl pyrrolidone resin, or maleic anhydride copolymer 4
Examples include synthetic resins such as tl[i; cellulose derivatives such as methylcellulose, ethylcellulose, hydroxyethylcellulose, and carboxymethylcellulose; natural polymers such as starch, polysaccharides, gelatin, casein, and sodium alginate. The binding material is 0.1 to 2% of the solid content of the composition for detecting occult blood.
It is desirable to use it in a proportion of 0% by weight, preferably 0.5 to 10% by weight. Water-absorbing powderWater-absorbing powder increases the water absorption of the test area formed by coating and drying on a support, promotes contact between the test liquid and the reagent composition, and enhances the color reaction of the support agent. Has a plow that facilitates. As such water-absorbing powder, it is not preferable to use one that shows extreme acidity or alkalinity when it comes into contact with water.
Moreover, those with high whiteness are preferable. Specifically, the following can be mentioned. Kaolin, synthetic silica, glass, cellulose sprock,
Microcrystalline cellulose, ion exchange cellulose, ion exchange resin, calcium carbonate, magnesium carbonate, aluminum silicate, etc. can be used. The water-absorbing powder has a content of 30 to 30% of the solid content of the composition for detecting occult blood.
It is desirable to use it in a proportion of 90% by weight. Preferably, the solvent is one that can uniformly and stably dissolve or disperse each of the above components, especially the binder. Solvents that meet these conditions include: ■ aromatic hydrocarbons such as benzene and toluene; ■ aliphatic ketones such as methyl ethyl ketone; ■ esters such as ethyl acetate; ■ non-aqueous solvents such as alcohols such as n-butanol. Alternatively, (1) water, or a mixture of (1) to (2) may be mentioned. However, for the composition for detecting occult blood, it is preferable to use a non-aqueous solvent because the drying process after coating on the support can be carried out at a low temperature and in a short time. Use of a non-aqueous solvent also has the effect of preventing alteration and deterioration caused by residual components of the composition when applied onto the specimen. Other Components Depending on the case, a wetting agent may be added to the composition for detecting occult blood in addition to the above-mentioned components. Examples of wetting agents include nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants, and polyethylene glycols. Wetting agents can help disperse each reagent, promote the formation of a uniform test area, and improve water wettability. The wetting agent is 0.5 to 5% by weight based on the solid content of the composition for detecting occult blood.
It is preferable that Φ exists. Further, in order to make the color tone of the support agent more visible, a background dye such as oil yellow may be added. Manufacture of Test Body The composition for detecting occult blood as described above is applied as an ink composition onto a support, and then dried to form an area for detecting occult blood and obtain a test body. Application methods include printing methods, coating methods (for example,
Roll coating method, spray coating method, dip coating method, solid coating method, etc. are used. In the present invention, the amount of the composition for detecting occult blood to be applied is relatively large, and it is preferable that the amount of application be constant, so the silk screen printing method is used. It is preferable to apply the composition for detecting occult blood onto a support by an intaglio printing method, a gravure printing method, or the like. The amount of the composition for detecting occult blood applied onto the support depends on the type of the composition, but is generally preferably 2 to 150 g/rtl (dry). Support The support is preferably one that does not react with the composition for detecting occult blood and does not inhibit the color of the indicator. Specifically, paper 2 is a laminate such as synthetic paper, non-woven fabric, synthetic resin film, or a combination of paper and synthetic resin film, which are composites thereof. A test object having an occult blood detection area formed on such a support may be formed into a stick shape, a roll shape, a tape shape, or the like. Alternatively, the support itself may be in a form in which the test liquid can be collected, for example, a cup-like shape, a test tube shape, 2 dish shapes, 2
It may be formed into a tray shape or a dropper shape. Hereinafter, the preparation of the composition for detecting occult blood and the production of test strips of the present invention will be described with reference to Examples. Example 1 Among the compositions for detecting occult blood described below, cumene hydroperoxide and 6-medoxyquinoline, which is a sensitizer, were microencapsulated using gum arabic, and other components were added to form a composition for detecting occult blood. Prepared. Next, the composition for detecting occult blood was finely dispersed with a homomixer, and a square test reagent part with one side of 5rRII+ was printed on a white polystyrene sheet with a thickness of 300 μm using a screen printing method, and after printing, it was heated at a temperature of 60° C. for 40 minutes. Dry. The number of lines of the plate used for printing was 80 meshes, and the total thickness of the resist and screen gauze was 130 μm. It was dried and cut into stick shapes of predetermined dimensions to obtain test specimens. Composition for detecting occult blood Cumene hydroperoxide: 3.6 parts by weight 6-medoxyquinoline 1.0 parts by weight (hereinafter in the capsule) Gum arabic 9.4 Toyobe 3
．． 3', 5.5'-Tetramethylbenzidine...1.5ΦΦ part Citric acid...0.56 Ir
! Hanging part Butrium citrate...2.2
Small base: triethanolamine lauryl sulfate...1.62 Overlay: polyethylene glycol...2.・52-layer polyvinyl butyral ...3.6-layer f
Part i (V4 Mizu Kagaku Co., Ltd., S-LEC BX-1> Cellulose fine powder...22.4 parts by weight (Seikasei Co., Ltd., Avicel TG-, D) n-amyl alcohol...39.71 hanging part butyl cellosolve acetate ・...13.0 Hanging part Test liquid: ■Normal urine■Urine containing 0.0611g/old human hemoglobin (manufactured by Sigma)■Urine containing 0.15 IIy/dl of human hemoglobin (manufactured by Sigma).■ Four types of urine containing 0.75 ag/dl human hemoglobin (manufactured by Sigma) were prepared, and the test specimens obtained above were
It was immersed in the liquid to be tested (2), taken out immediately after immersion, and left to stand for 1 minute, after which the seven colors of the test reagent part were observed. The seven colors of the test reagent part are uniform and clear, and the color density increases step by step as the amount of human hemoglobin in the test liquid increases, and the concentration of occult blood in the test liquid increases within the above range. was clearly distinguishable. No change in color tone was observed even when the yellow-colored test specimen was allowed to stand at room temperature for 5 minutes. In addition, the obtained test specimen was sealed in a glass container,
After storing it at 40℃ for 12 months, we performed the same test as above, and found that the color of the test reagent part was as clear and stable as before storage, and the concentration of the blush method in the test liquid was preserved. As before, it was possible to distinguish clearly. Comparative Example 1 The cumene hydroperoxide and 6-medoxyquinoline shown in Example 1 were not microencapsulated, and the same lff1
A test specimen was produced in the same manner as in Example 1 except that cumene hydroperoxide and 6-medoxyquinoline were added. However, the test reagent part was stained blue after drying at 60° C. for 40 minutes. In addition, when testing was carried out using the liquids to be tested from ■ to ■ used in Example 1, it was difficult to clearly determine the hemoglobin concentration in the liquids to be tested in the low concentration range due to contamination during drying. . Furthermore, the manufactured test specimen was placed in a glass container tightly.
After storing it at 40℃ overnight, we performed the same test as above, and compared to before storage, the color of the shell of the test reagent part became unrestricted and unrecognizable, and there was no evidence of hemoglobin in the test liquid. It was also difficult to determine the concentration. Example 2 Regarding the composition for detecting occult blood in Example 1, in addition to cumene hydroperoxide, 6-medoxyquinoline as a sensitizer, triethanolamine lauryl sulfate, polyethylene glycol as a wetting agent, and polyvinyl butyral as a resin were added. A part of the test piece was microencapsulated using gum arabic, and each component was used as shown below, and a test specimen was subjected to tJFj in the same manner as in Example 1. It showed the same coloration and had a good shelf life. It was good. 1 Kuze ■ 1 Cumene hydroperoxide ・・・3.6ffi
ffi part 6-medoxyquinoline...1
, 0 parts by weight Lauryl sulfate triethanolamine... 1.62 f
flffl part polyethylene glycol...2
．． 52 Polyvinyl butyral...
・0.2fi 1 part (Sekisui Chemical, S-LEC BX-1)
(inside the capsule) gum arabic
...9.4ffiffi section 3.3', 5.5
- Tetramethylbenzidine...1.5 parts Citric acid...0.56 parts Sodium citrate...2.11 parts Polyvinyl butyral...3.6 parts (Sekisui Chemical) manufactured by ESLETSUKU BX-1) (Outside the capsule) Cellulose fine powder...22.
Quadruple hanging part (manufactured by Fukkasei, Avicel TG-0) n-amyl alcohol...39.7 parts by weight Butyl cellosolve acetate...13.0
1°C 1 part Comparative Example 2 Regarding the composition for detecting occult blood in Example 1, in addition to cumene hydroperoxide and 6-medoxyquinoline, 3,3',5,5'-tetramethylbenzidine, which is an oxidizable indicator, was added. Microcapsules were prepared using gum arabic, and test specimens were crushed in the same manner as in Example 1 using each component as described below. Wi blood detection compound cumene hydroperoxide...3.6ffi
Part 1 6-Medoxyquinoline...1.0
Parts by weight 3.3', 5.5' - Tetramethylbenzidine...1.5 parts by weight (more than that in the capsule) Gum arabic...9.4 parts by weight Citric acid...0.56 parts by weight Citric acid Sodium acid...2.2 double ff
Part i Lauryl sulfate triethanolamine...1.62 In
Part polyethylene glycol...2.52 parts Polyvinyl butyral...3.6 parts by weight (Sekisui Chemical, S-LEC BX-1> Cellulose fine powder...22.4% @
Parts (Avicel TG-D manufactured by Seikasei) n-amyl alcohol...39.7 parts by weight Butyl cellosolve acetate...13.01
However, the test reagent portion was stained blue after drying at 60° C. for 40 minutes. In addition, when testing was carried out using the liquids to be tested from ■ to ■ used in Example 1, it was difficult to clearly determine the hemoglobin concentration in the liquids to be tested in the low concentration range due to contamination during drying. . Furthermore, after sealing the prepared test specimen in a glass container and storing it at 40°C overnight, the test was performed in the same manner as above. It became unclear and unclear, and it was difficult to distinguish hemoglobin in the test liquid at once. Comparative Example 3 Regarding the composition for detecting occult blood in Example 1, cumene hydroperoxide, 6-medoxyquinoline, and buffering agents citric acid and sodium citrate were microencapsulated using gum arabic, and each component was prepared as follows. A test piece for inspection was manufactured in the same manner as in Example 1 using the same method as in Example 1. 1Agura Kodan 1111jill Cumene hydroperoxide...3.6ffi
ffi part 6-medoxyquinoline...1
．． 0 weight 41 parts citric acid...0
．． 56 Juteito Sodium Citrate...
2.2 parts by weight (or more in the capsule) Gum arabic...9.4 parts by weight 3.3',S,5'-tetramethylbenzidine...1.5 parts by weight lauryl sulfate triethanolamine...・1.62 parts by weight Polyethylene glycol 2.5211 parts Polyvinyl butyral 3.6 parts (Sekisui Chemical Co., Ltd., S-LEC BX-1> Cellulose fine powder 22.4 ff
! ffi part (Fushikasei, Avicel 10-D) n-amyl alcohol...39.7 weight f
fi part butyl cellosolve acedate...13.
0ffiffi part However, when testing was carried out using the test liquids (■ to ■) used in Example 1, it was difficult to clearly determine the hemoglobin concentration in the test liquid in the low concentration range due to contamination during drying. there were. Furthermore, the manufactured test specimen was sealed in a glass container,
When the bond was stored overnight at 40°C and tested in the same manner as above, the color of the test reagent part became unreliable and unclear compared to before storage, making it difficult to determine the concentration of hemoglobin in the test liquid. Met. Example 3 Of the occult blood detection composition of Example 1, 3,3',5,5'-tetramethylbenzidine, which is an oxidizable indicator, and citric acid and sodium citrate, which are pHfl buffer agents, were mixed with gum arabic. The peroxide, cumene hydroperoxide, and the sensitizer, 6
- A test piece for occult blood testing was prepared with the composition shown below without medoxyquinoline being microencapsulated. Number @ i'lf m fill ash 1 3.3', S, 5'-tetramethylbenzidine...1.5fif
Part i citric acid...0,56Φ
Hanging part Sodium citrate...2.21
ff1 part (more than that in the capsule) Gum arabic...9.4 parts by weight Cumene hydroperoxide...3.3 parts by weight 6-medoxyquinoline...0.9 parts by weight Lauryl sulfate triethanolamine...1 .62Φ presentation part polyethylene glycol...2.52 double claw part polyvinyl butyral...3.6il
qfi part (Sekisui Chemical, S-LEC BX-1) Cellulose fine powder...22.4ff
! Hanging part (manufactured by Fukkasei, Avicel TG-D) n-amyl alcohol...39.711
Butyl cellosolve acetate...13. OI
When the test liquid was tested in the same manner as in Example 1, the two colors of the test reagent were uniform and bright, and the color concentration was the same as in Example 1. As the human hemoglobin concentration increased, the concentration of occult blood in the test fluid could be clearly determined. No change in color tone was observed even when the seven-colored test specimen was allowed to stand at room temperature for 5 minutes. In addition, the blued test specimen is sealed in a glass container,
After being stored at 40°C for 12 months, a test was conducted in the same manner as above, and the seven colors in the test reagent part were as uniform, clear, and stable as before storage, indicating that there was no trace of H in the test liquid. It was possible to clearly determine the hemoglobin concentration as before storage. Comparative Example 4 Of the occult blood detection composition of Example 3, 3,3',5,5'-tetramethylbenzidine, which is an oxidizable indicator, was microencapsulated using gum arabic, and cumene, which was a peroxide, was microencapsulated using gum arabic. Hydroperoxide and sensitizer 6-
A test piece for detecting occult blood was prepared without microencapsulating medoxyquinoline and having the composition shown below. Occult blood detection composition 3.3', 5.5'-tetramethylbenzidine...1.5flj
1 part (or more in capsule) Gum arabic...5.4 parts by weight Cumene and droperoxide...3.31 parts 6-medoxyquinoline...0.9 parts by weight Citric acid...0.56 Part by weight Sodium citrate...2.211
Part lauryl sulfate triethanolamine...1.62 fI
ffiI polyethylene glycol...2.5
2ff! ffi part polyvinyl butyral
...3.6 parts by weight (Sekisui Chemical, S-LEC BX-1
) Cellulose fine powder...22.4 cup part (Fushikasei, Avicel TG-D) n-7 mil alcohol...39.7 part Butyl cellosolve ahitate...13.0Φ
However, after sealing the manufactured test specimen in a glass container and storing it at 40°C for 12 months, a test was conducted using the test liquid in the same manner as in Example 1. In comparison, the two colors of the test reagent part were uneven and unclear, making it difficult to distinguish the concentration of hemoglobin in the test liquid. Effects of the Invention In the present invention, in order to prevent the organic hydroxyperoxide added to the composition for detecting occult blood from coming into contact with other components in the composition and being destroyed, organic hydroxyperoxide is added to the composition for detecting occult blood. Microencapsulate components that do not react with peroxides or hydroperoxides, or oxidizable indicators that react with organic hydroperoxides! 1v
Since we use microencapsulated iI agent,
By applying the composition for detecting occult blood onto the indicator and drying it, the test object can be manufactured without contamination, and
It is possible to prevent the organic hydroxyperoxide from coming into contact with an indicator or an Ig buffer and being decomposed during storage. In addition, at Kikomei, quinoline and triethanolamine salt are added to the composition for detecting occult blood, so the sensitivity and sensitivity can be improved. It can improve color density and enable semi-quantification of hemoglobin and red blood cells in a low concentration range.
In the embodiment of the present invention, since a water-soluble polymer is used as the coating material for the microcapsules, the lf of the coating is
It also has the advantage of rapid breakdown and rapid reaction. Furthermore, in the present invention, the occult blood detection area can be formed by a printing method rather than by direct coating on the support, which is advantageous for hanging production and can simplify the manufacturing process. Patent applicant Dai Nippon Printing Co., Ltd. agent Patent attorney Katsu Ohno Agent Patent attorney Reiko Ohno Agent Patent attorney Ryunosuke Ohno procedural amendment (voluntary) Commissioner of the Japan Patent Office Kunio Ogawa Tono 1, case Indication of 1986 Patent Application No. 231779 2 Name of the invention Composition for detecting occult blood and test body 3 coated with the composition 3 Relationship with the person making the amendment Patent applicant address Shinjuku-ku, Tokyo Ichigaya Kagacho-chome 1-1 Name (289) Dai Nippon Printing Co., Ltd. 4, Agent 1, Akira [1, page 123, insert the following sentence between lines 17 and 18. [Also, place the obtained test specimen in a glass container tightly.
After storing it for 12 months at 40°C, we conducted the same test as above, and found that the test reagent part had ? The color was uniform, clear, and stable as before storage, and it was possible to clearly distinguish the human hemoglobin S degree in the test Ii liquid as before storage. In addition to the test subjects described in ■ to ■ above, normal urine, 20 red blood cells/μ℃ in normal urine, and 250 red blood cells/μ11 in normal urine were used as test liquids. When colored in the same manner, clear and stable spots appeared on the test reagent part, and the number of spots gradually increased as the FIA degree of red blood cells in the test liquid increased.
It was possible to clearly determine the concentration of red blood cells in the test liquid within the above range. No change in color tone was observed even when the nine-colored test specimen was allowed to stand at room temperature for 5 minutes. ” 2. Fill out the power of attorney as shown in the attached sheet.

Claims (1)

[Scope of Claims] 1. An organic hydroperoxide, an oxidizable indicator capable of producing a detectable response in the presence of the organic hydroperoxide and a peroxide active substance, a sensitizer, a pH buffer, a binder, and a composition for detecting occult blood in which a water-absorbing powder is dissolved or dispersed in a non-aqueous solvent, the components being a first group containing an organic hydroperoxide and a second group containing an oxidizable indicator. A composition for detecting occult blood, characterized in that either the first group or the second group is microencapsulated with a water-soluble microcapsule coating material. 2. The first group is an organic hydroxide, a sensitizer,
Claim 1, characterized in that the organic hydroperoxide alone or the organic hydroperoxide and any other component belonging to the first group are microencapsulated. The composition for detecting occult blood according to section 1. 3. The second group consists of an oxidizable indicator, a pH buffer, a water-absorbing powder, and a part of the binder, and the oxidizable indicator alone or the oxidizable indicator and others belonging to the second group The composition for detecting occult blood according to claim 1, wherein any constituent component of the composition is microencapsulated. 4. The composition for detecting occult blood according to any one of claims 1 to 3, wherein the coating material used for microencapsulation is a water-soluble polymer. 5. Claim 4, characterized in that the water-soluble polymer is gum arabic, carboxymethyl cellulose, polyvinyl alcohol, or a mixture thereof.
The composition for detecting occult blood as described in . 6. The composition for detecting occult blood according to claim 2, wherein the sensitizer is quinoline or a derivative thereof and a triethanolamine salt. 7. The composition for detecting occult blood according to claim 6, wherein the triethanolamine salt is triethanolamine lauryl sulfate, or triethanolamine polyoxylauryl sulfate alone or in a mixture. 8. from an organic hydroperoxide, an oxidizable indicator capable of producing a detectable response in the presence of the organic hydroperoxide and the peroxide active substance, a sensitizer, a pH buffer, a binder, and a water-absorbing carrier powder. A composition for detecting occult blood, wherein one of the first group containing an organic hydroperoxide and the second group containing an oxidizable indicator is formed by a water-soluble microcapsule coating material. A test object for detecting occult blood, characterized in that a microencapsulated composition is coated on a support. 9. The first group consists of an organic hydroperoxide, a sensitizer, and a part of the binder, and the organic hydroperoxide alone or the organic hydroperoxide and any other component belonging to the first group The test object for detecting occult blood according to claim 8, characterized in that it is microencapsulated. 10. The second group consists of an oxidizable indicator, a pH buffer, a water-absorbing powder, and a part of the binder, and the oxidizable indicator alone or the oxidizable indicator and others belonging to the second group 9. The test object for detecting occult blood according to claim 8, wherein any constituent component of the test object is microencapsulated. 11. Claims 8 to 1, characterized in that the coating material used for microencapsulation is a water-soluble polymer.
The specimen for detecting occult blood according to any one of item 0. 12. The test object for detecting occult blood according to claim 11, wherein the water-soluble polymer is gum arabic, carboxymethyl cellulose, polyvinyl alcohol, or a mixture thereof. 13. The test material for detecting occult blood according to claim 8, wherein the sensitizer is quinoline or a derivative thereof and a triethanolamine salt. 14. The test object for detecting occult blood according to claim 13, wherein the triethanolamine salt is triethanolamine lauryl sulfate or triethanolamine polyoxylauryl sulfate alone or in a mixture.