JPH01294637A - Method and apparatus for collecting immune antibody and complement - Google Patents

Abstract

PURPOSE:To provide an immune antibody and a complement useful for the treatment of man infected with pathogens by removing the pathogens form urine collected from the infected man himself or any other man infected with the same pathogens or subjecting the urine to an inactivation treatment. CONSTITUTION:Urine is collected from an infected man himself or any other man or animal infected with the same pathogens. The collected urine is filtered with a filtering material capable of removing the pathogens or the pathogens in the urine are inactivated with a physical or chemical treatment capable of being carried out without damaging the immune antibody and complement. The treated urine is further passed through a dialyzing apparatus capable of removing urea during or after the aforementioned treatment to diffuse and remove the urea, thus collecting the immune antibody and complement.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a method and apparatus for collecting immune antibodies useful for treating people infected with viruses, bacteria, microorganisms, and other pathogens.

<Conventional technology> Modern medicine has developed special drugs such as antibiotics that act directly against some bacteria, and has brought good news to those infected with those bacteria. However, modern medicine is nearly powerless against bacteria for which specific drugs have not yet been developed, resistant bacteria and microorganisms for which specific drugs are no longer effective, and all pathogenic viruses, while adding symptomatic treatment.
The current state of general medical care is to leave it to the patient's own natural healing power.

In physiology and pathology, the natural healing power of the human body has been discovered and elucidated.

That is, when an animal is invaded (infected) by a pathogen, it produces cellular and humoral immune responses. The former is a reaction in which cells such as white blood cells and macrophages strengthen their attack power against the pathogen, and the latter is a reaction in which antibodies that are effective against the pathogen are generated in the plasma, and these act as the infected person's natural healing power. There is.

Conventionally, methods to artificially strengthen this natural healing power include using immune serum taken from the blood of a person or animal infected with a specific pathogen for testing and treatment, or by giving weakened pathogens in advance to provide immunity before infection. It seems that there is only a way to use a vaccine with increased potency or to add the vaccine to the above immune serum for treatment.

In addition, the oriental medicine and folk medicine urinary therapy that inspired this invention include ``drinking your own urine to cure incurable diseases'' and ``every day.
Teacup - drink the urine as it is, it's your own urine so it won't harm you.''

<Problem to be solved by the invention> To overcome the current state of general medical care, where patients infected with pathogens for which there are no specific drugs are treated symptomatically and wait for natural recovery, and to provide a new means of enhancing natural healing power. This is the purpose of this invention.

<Means for solving the problem> The method for collecting immune antibodies and complement of the first invention is based on the method of collecting immune antibodies and complements from the infected person himself or herself.
It is characterized by collecting the urine of another person or animal infected with the same pathogen and filtering the urine through a filter medium that can remove the pathogen.

The immune antibody and complement collection method of the second invention is based on
It is characterized by taking the urine of another person or animal infected with the same pathogen and inactivating the pathogen in the urine by physical or chemical treatment that can be performed without damaging immune antibodies or complement. do.

The immune antibody and complement collection method of the third invention includes, during or at the end of the method according to claim (1) or claim (2),
It is characterized by passing the urine through a dialysis machine that can remove urea.

The immune antibody and complement collection device of the fourth invention comprises a filtration device that has a urine supply port and uses a filtering medium capable of removing bacteria or viruses in the urine, and a filtration device that allows the urine discharged from the filtration device to pass through. A dialysis device capable of diffusing and removing urea in urine,
It is characterized by having the following.

<Function> This invention has elucidated the ancient method of urinary therapy in Oriental medicine and folk medicine using modern physiology, eliminated its drawbacks, and opened the way to use urine as a source for collecting immune antibodies and complement.

The reason why medical scientists have not seriously taken up folk urine therapy in the past is probably because immune antibodies and complement in urine are about one-tenth the size of viruses, making them difficult to detect even with an electron microscope. This is probably because there was no way to confirm its existence. Furthermore, although unnecessary substances in the blood are excreted as urine due to physiological processes, it is not recommended for patients who are particularly physically weak to drink that urine and return it to the blood, thereby disrupting the flow of physiological processes. If there is any effect, it may be due to the belief of medical scientists that it may be due to the psychological effect of the religious feeling of drinking dirty urine. Furthermore, even if urine therapy is successfully recommended to patients, the fact that it does not generate any income or profits for the doctor seems to be killing their motivation.

It would be unscientific to not discuss folk urine therapy because it cannot be confirmed that urine contains immune antibodies and complement.

This invention is based on modern physiology, proves the necessity for urine to contain immune antibodies and complement, and also clarifies the shortcomings of conventional urine therapy.

First of all, regarding the necessity mentioned above, it is widely accepted that when an animal is infected with a pathogen, immune antibodies are produced in the blood.

Whether the antibody kills the pathogen with the support of phagocytes and complement,
It is also well known to envelop.

Naturally, pathogens that have been killed or encapsulated must be transported out of the body, but the only way to transport them out of the body is through feces or urine. Pathogens that are killed or encapsulated in the affected tissue are transported in the lymphatic and blood vessels to the kidneys. The function of the kidneys is complicated and mysterious, as it repeats filtration and absorption, and as a result, waste products, excess substances, foreign substances, and pathogens carried in the blood are excreted in the urine along with large amounts of plasma. As is well known, pathogens that have been killed or encapsulated are swarmed with immune antibodies and complement, and given that the kidney's filtration function is relatively rough, antibodies and complement can be found in urine. It is inevitable that it will come out.

Based on the above, it is theoretically certain that antibodies produced in response to the pathogen that caused the infection and complement that participated in the fight are present in the urine of an infected person.

Antibodies and complements released in urine can be thought of as consumables, like bullets that attack pathogens, so even if they are collected and returned to the body, they may not immediately become an effective fighting force. However, even if damaged antibodies and complement are absorbed into the body, they will be sent to antibody-producing tissues such as lymph nodes, spleen, and bone marrow, where they will be quickly restored to their complete form. The undeniable therapeutic effect of folk remedies can only be attributed to the fact that by drinking the patient's own urine, which contains antibodies and complement, those antibodies and complement return to the plasma and participate in the attack of the same pathogen.

The functions of immune antibodies in plasma seem to be diverse, and according to the literature, they precipitate soluble antigens (pathogens), aggregate particulate antigens, promote phagocytosis of bacteria, neutralize toxins, and increase susceptibility. It says that bacteria with this type of bacteria cause cell collapse. The definition is, ``Antibody is a general term for substances that react specifically with antigens.'' Considering this, even if antibodies and complements gather, it is rather unlikely that they will completely kill and transport pathogens; In many cases, live pathogens may be excreted in the urine.

This is another disadvantage of conventional urine therapy, which returns live pathogens to the body at the cost of recycling antibodies and complement.

Another disadvantage of conventional urine therapy that cannot be overlooked is that it passes through the urinary tract, which is susceptible to infections such as nephritis, pyelitis, and cystitis, making it easy for new pathogens to be added to the urine.

This invention involves collecting the urine of an infected person and removing all pathogens in the urine, including those carried out from the tissues and those added from the urinary tract (first invention), or by removing antibodies and complement. (Second invention). This eliminates the biggest drawback of conventional therapy.

The urine collected in this way has the same function as plasma, which contains antibodies and complement, except that it contains a large amount of waste products such as urea, which are harmless in themselves. , can enhance complement all at once.

Although the urine collected according to the first and second inventions has removed or inactivated pathogens, it contains a large amount of waste products such as urea. Increased blood concentration causes physical discomfort. This is also a drawback of conventional urine therapy. Either the kidney itself has sufficient processing capacity to handle the problem even if only one side is affected, or there is a possibility that there will be a delay in dealing with the sudden increase in urea concentration.

The third invention seems to have solved this problem. That is, the first
．． The urine collected and processed in the second invention is passed through a dialysis machine either midway or finally, and urea in the urine is diffused and removed, similar to artificial dialysis for kidney disease patients. This dilutes the urea in the urine enough that it can no longer be called urine, but a liquid with components similar to plasma.

The details of conventional urine therapy, the size differences between various pathogens, antibodies, and complement, and the size of urea that made the filtration and dialysis of the present invention possible are described in the next section.

A fourth invention provides an apparatus for carrying out the sampling method of the third invention.

<Example> First, as an example of the latest information on urinary therapy conventionally practiced in the private sector, the following is an excerpt from a memo by doctor Ryo Nakao that was published in the April 1986 issue of the magazine ``Sokai''. show.

[In the case of gonorrhea patients] ``The next morning, I drank a cup of urine as I was told, and from that night on, the burning pain in my urethra when I urinated disappeared. is.

Within two or three days, this patient was completely cured of his stubborn gonorrhea. ” [Letter from a fellow doctor who contracted herpes] “While I was suffering from herpes zoster (herpes zoster) for about three weeks, I received a phone call from Nakao-nii from Kofu, telling me to drink balun (urine). I drank mine, prepared to jump off the stage in Shimizu in agony.I suddenly recovered!
What's more, the gout in my left big toe has also been cured. No, but hats off to Nakao-nii. ” [Rheumatology patient (doctor’s own medical record)] “- times of urine drinking was 180 m1
, twice on the first day, once on the second day, the pain improved once, and the urine output increased. The herpes rash was cured by taking the above dose. What I didn't expect was that at the same time, the gout in my left big toe, which I had been suffering from for a long time, started to heal. ” This is a doctor's note, and the two patients are fellow doctors, so they can be trusted. I think there may be some cases where it didn't work or caused harm, but I don't have any data on that.

Now, what is shown in the drawings is an embodiment of the fourth invention for carrying out the sampling method of the third invention. Remove the dialysis device 3 and use the filtration device! If the receiver drops the liquid directly into the cup 4 from t2, the collection method of the first invention can also be carried out.

1 in the figure is the urine supply port, which has a capacity to store at least 180ml of urine. 2 is a filtration device, which uses a porous hollow fiber membrane made from recycled cellulose as a filter material that can remove both bacteria and viruses. The dimensions of this hollow fiber membrane are an outer diameter of 240 to 300 pm and an inner diameter of 200 to 210 g.
m, 119425~30 gm, and 200 gm in this film thickness.
It is multi-layered with ~300 people or 100-150 layers. The membrane has 1 billion holes per 1 cya, and each layer of the membrane sequentially traps viruses and completely separates them.

Complete separation is made possible by using ultra-multilayered layers.

The filtration rate will be hundreds of times faster than that of an artificial kidney. The recycled cellulose material does not absorb proteins, so it does not clog the holes, and it also absorbs albumin and immunoglobulins (antibodies).
It is a filter material with good membrane permeability for useful proteins such as

For reference, the size of each component in blood is shown in numerical digits: red blood cells are 10-61, bacteria are 10-'m, viruses are 10-'m to 1G-'m, and immune antibodies (globulins). is 10-9 m. The dimensions of urea and complement cannot be found in the literature, so if we compare them with the above antibody in terms of molecular weight, the molecular weight of the antibody is 160,000 to 190,000, and the molecular weight of complement is 60,000 to 590,000, whereas the molecular weight of urea is only 60.1. .

Accordingly, the hole opening of the filter medium used in the first invention is 10-'s if the infected pathogen is a bacteria, and 10-'m if the infected pathogen is a virus. In either case, the size is such that bacteria that enter the urinary tract due to a urinary tract infection can be eliminated.

The urine flow inlet at the upper end of the dialysis device 3 is connected below the filter N2 in the figure. Urine that descends from the urine supply port 1 and from which pathogens have been removed while passing through the filter medium in the filtration device 2 immediately enters the upper part of the dialysis device 3 .

The dialysis device 3 uses a ready-made product for hemodialysis. There are dozens of types, and they are roughly divided into three types: coil type, laminated type, and hollow fiber type. In the coil type, a long, thin envelope-like membrane is rolled into a rolling paper, and blood (in this invention, urine) flows into the envelope, which is opened at the top and bottom, and dialysate (usually water) flows outside.

The laminated type looks like a stack of envelopes like a sandwich, and the hollow fiber type has a bundle of capillary glass tubes.

When urine is put into the blood side of these mass-produced or custom-made hemodialyzers and water is sent to the dialysate side, due to the concentration difference between both sides, urea in the urine or a semi-permeable membrane (polymer membrane such as cellophane) The solution penetrates and diffuses into the dialysate water, so you can just throw that water away.

In the illustrated embodiment, a water tank 9 is fixed under the water faucet 8, and the dialysate (
water) descend to the inlet 3a, and from the upper outlet 3b the hose 1
0' points toward the drain.

The device shown in the figure is the simplest one for general household use, with a support 6 erected on a base 5 on which a cup 4 is placed, and three supporting arms attached to it, and connected to the body. Urine supply port 1, filter device 2. The dialysis device 3 is supported and the liquid is dripped into the cup 4 from the purified liquid outlet 11 at the lower end, and the method of supporting the water tank 9 is also arbitrary.

Since dialysis takes time, ice blocks are placed in the water tank 9 to keep the temperature of the water entering the inlet 3a as a dialysate at 5 to 6°C in order to prevent the urine from deteriorating. The outer periphery of the filter device 2 may be cooled with water.

The pressure on the urine side of the dialysis device 3 may be based only on gravity, but
To speed up the dialysis rate, air pressure may be applied to the upper surface of the urine in the supply port 1, or pressure may be lowered using a piston with a weight placed on it. If the urine is lowered only by zero gravity, the water tank 9
Make sure that the water level is lower than the urine supply port 1.

As mentioned above, the dialysis machine 3, water tank 9, hose 1O1 in Figure 1
If 10' is removed, the first invention can also be carried out.

The second invention, unrelated to the diagram, involves subjecting the material taken from an infected person to physical or chemical treatments to inactivate pathogens that have survived in the urine or have been added in the urinary tract. Physical methods include heat treatment and irradiation.

Heat treatment is for relatively heat-sensitive antibodies and complement (56℃, 20~
The pathogens that can be used are therefore limited.

Irradiation is a treatment method that is still under development, and currently radiation is only used to disinfect seeds. However, future research and development will make it possible to kill pathogens, which are the only living organisms in urine, by irradiating them with radiation, electron beams, ion beams, etc., or at least make them incapable of multiplying! ! (inactivation) seems to be easy.

The method of inactivating pathogens in urine by chemical treatment is
After killing the pathogen by adding an acid or alkali, we add a neutralizing agent to return it to neutrality.If the pathogen is a bacteria, we add an antibiotic or a specific medicine, if it is a virus, we use interferon to inactivate it, and other things in the future. All you have to do is add anti-virus drugs that are developed.

<Effects of the Invention> This invention has almost achieved the inventor's wish to provide an effective countermeasure to humanity suffering from viruses, bacteria, microorganisms, etc.

Since the immune antibodies produced in the bodies of humans and animals correspond to each infectious pathogen, the antibodies and complement-containing substances collected by this invention can serve as a natural healing power enhancer for all infectious disease patients. obtain.

This invention has opened the way for the collection of immune antibodies and complement, which are extremely effective against pathogens, from the urine of infected people and infected animals, which is the cheapest material, using an inexpensive collection device, and will benefit poor infectious disease patients around the world. , which allows people to receive the latest and most suitable medical care at the lowest medical costs.

[Brief explanation of the drawing]

The figure shows an embodiment of the immune antibody and complement collection ttM of the present invention. In the figure, 1 is a urine supply port, 2 is a filtration device, and 3 is a dialysis device. Patent Applicant Tetsuo Takano Procedural Amendment (September 7, 1988)

Claims (4)

[Claims] (1) A method for collecting immune antibodies and complement, which is characterized by collecting urine from an infected person or from another person or animal infected with the same pathogen, and filtering the urine through a filter medium capable of removing the pathogen. (2) Taking the urine of the infected person or another person or animal infected with the same pathogen, and removing the pathogen in the urine by physical or chemical treatment that can be carried out without damaging immune antibodies or complement; A method for collecting immune antibodies and complement characterized by inactivation. (3) Collection of immune antibodies and complement characterized by passing the urine through a dialysis machine capable of removing urea during or at the end of the method according to claim (1) or claim (2). Law. (4) A filtration device that has a urine supply port and uses a filtering material that can remove bacteria or even viruses in the urine, and by passing the urine from the filtration device, urea in the urine can be diffused and removed. An immune antibody and complement collection device comprising: a dialysis device;