JP7016117B2 - Collagen cosmetics - Google Patents

Description

The present invention relates to a novel cosmetic product using collagen.

Collagen is a type of protein that makes up the skin and bones of animals. Several types of collagen are known, but among them, type I collagen forms the skin and bones of animals and is widely distributed. Type I collagen has a triple helix structure composed of one α1 chain and two α2 chains. When this type I collagen gathers, it becomes fibrous and is called collagen fiber.

Type I collagen is abundant in the dermis and supports its toughness and tension. Therefore, when type I collagen is insufficient, the skin becomes less tense, and it is considered effective to supplement this as a cosmetic product, and various skin care products have been developed.
Cosmetics containing collagen having a triple helix structure are generally liquid agents (essences), but since microorganisms easily propagate in collagen, it is necessary to add a preservative, which is problematic for cosmetics. A collagen porous body obtained by freeze-drying a triple-helical collagen aqueous solution is also used, but its mechanical strength is weak, and it can be molded into an arbitrary shape, and even if it is molded, its structure is maintained until use. There is a problem in use because it is difficult to store it as it is. In particular, film-like triple helix collagen is not known.

Regarding the application of type I collagen to cosmetics, cosmetics containing vitamin C in type I collagen (Patent Document 1), expression of CD44 membrane receptor in skin cells by type I collagen (Patent Document 2), type I collagen Skin wrinkle improving cosmetics (Patent Document 3) and the like using a peptide derived from collagen have already been published. In addition, type I collagen production promoters (Patent Documents 4, 5, and 6) have also been studied.

Japanese Unexamined Patent Publication No. 2001-139447 Special Table 2002-540126 Publication No. Japanese Patent Application Laid-Open No. 2003-277399 Japanese Patent Publication No. 2004/0854229 Japanese Unexamined Patent Publication No. 2011-006331 Japanese Patent Publication No. 2014-510052

An object to be solved by the present invention is to provide a novel cosmetic product or a composition thereof utilizing the excellent properties of triple helix collagen.

The cosmetic product of the present invention is characterized by being a triple helix collagen film made of triple helix collagen and a compatibilizer.
The present inventors have repeatedly studied to make cosmetics of a formulation type that is easy to use by taking advantage of the excellent properties of triple-helical collagen, and have reached a transparent or translucent film-like cosmetic that maintains the structure of triple-helical collagen. ..

In the present invention, the triple helix collagen refers to collagen that retains the same triple helix structure as type I collagen in living tissue. Whether or not the triple helix structure is maintained can be confirmed by measuring the specific rotation. The preferred specific rotation is −300 ° to −400 °. As the triple helix collagen, a commercially available product can be preferably used, and examples thereof include triple helix collagen Ti-03B (manufactured by Taki Chemical Co., Ltd.).

In order to dry the triple spiral collagen aqueous solution into a transparent or translucent film, it is necessary to coexist with a water-soluble polymer as a compatibilizer. The compatibilizer wraps the triple helix collagen in water and dissolves in the water, and when the water is dried and removed, it wraps the triple helix collagen to form a film. The coexistence of the compatibilizer gives the triple helix collagen mechanical strength and takes the form of a film. This film-like triple collagen is useful as a material for cosmetics.

The compatibilizer is preferably a polymer substance that satisfies the following conditions. The condition (1) is that it is water-soluble and can itself form a strong film by drying. Condition (2) has an affinity for triple-helical collagen and does not cause phase separation when coexisting with triple-helical collagen in water. The condition (3) is that it does not have a polar group such as a carboxyl group or an amino group, and does not cause an electrostatic interaction with the triple helix collagen. The condition (4) is that when the aqueous solution coexisting with the triple helix collagen is dried, a uniform film is obtained without causing a non-uniform pattern.

As the compatibilizer, dextran or starch can be preferably used, and dextran is more preferable. Polysaccharides such as hyaluronic acid and carboxymethyl cellulose cannot be used because they have a carboxyl group and interact with the triple spiral collagen amino group. The compatibility between the compatibilizer and the triple spiral collagen is higher when the molecular weight of the compatibilizer is lower, and the molecular weight of the compatibilizer is preferably 100,000 or less. It is desirable that the amount of the compatibilizer is 3 to 90 parts by mass with respect to 100 parts by mass of the triple helix collagen. If the amount of the compatibilizer is less than 3 parts by mass, the amount of the compatibilizer may be insufficient, and it is difficult for the dried product to be a smooth film. If the compatibilizer exceeds 90 parts by mass, when the triple helix collagen film is applied to the skin, even if water is added, the compatibility with the skin deteriorates.

In order to prepare a transparent or translucent triple-helix collagen film, it is desirable to coexist with a film enhancer in addition to the compatibilizer. The film-enhancing agent has a low affinity for triple-helical collagen, and a good film cannot be produced only by the film-enhancing agent. However, when coexisting with the compatibilizer, the film enhancer can increase the strength of the triple helix collagen film. As the film enhancer, a polymer substance similar to the compatibilizer can be used, but (1) the compatibility with triple spiral collagen may be lower than that of the compatibilizer, and (2) the molecular weight is higher. It has the characteristics that it can be expensive. If a film enhancer is added, the triple helix collagen film can be easily handled and the physical characteristics as a practical product are improved.

As specific examples of the film enhancer, hydroxypropyl cellulose, polyvinyl alcohol, polyvinylpyrrolidone and the like are effective, and hydroxypropyl cellulose is particularly preferable. Since the purpose of the film enhancer is to improve the mechanical strength of the film, it is desirable that the film has a high molecular weight, and more specifically, the molecular weight is preferably 100,000 or more. It is desirable to use 0.1 to 40 parts by mass of the film enhancer with respect to 100 parts by mass of triple helix collagen. If it is less than 0.1 parts by mass, it may not be effective as a film enhancer. If it exceeds 40 parts by weight, it may not be suitable as a cosmetic product due to its poor solubility even when water is applied to the product when it is applied to the skin.
As the film-enhancing agent, cellulose nanofibers can be preferably used in addition to the water-soluble polymer as described above. Although cellulose nanofibers are not water-soluble polymers, they are dispersed in water as extremely small particles and interact with the particles by the hydroxyl groups of cellulose to form a tough film when dried, so that they are suitable as the film enhancer of the present invention. As with the water-soluble polymer, the amount of cellulose nanofibers added is preferably 0.1 to 40 parts by mass with respect to 100 parts by mass of triple helix collagen. As the cellulose nanofiber, for example, Leocrysta C-2SP manufactured by Dai-ichi Kogyo Seiyaku Co., Ltd. can be preferably used.

It is desirable that a water-soluble plasticizer coexists as other components. The role of the water-soluble plasticizer is to plasticize the transparent or translucent triple-spiral collagen film and make it more soluble in water after being applied to the skin. As the water-soluble plasticizer in the present invention, polyhydric alcohols such as glycerin, ethylene glycol and propylene glycol are suitable, and N-methylpyrrolidone can also be used.

Even if an attempt is made to simply dry the triple spiral collagen aqueous solution without using a compatibilizer or the like to prepare a film, the product does not become brittle and film-like. The cause is that one chain constituting triple spiral collagen has a molecular weight of about 100,000, but each of the three chains having a molecular weight of 100,000 has a spiral structure, and the structure does not collapse even in water, so collagen. This is probably because the molecular chains of each other do not get entangled at all.

The triple helix collagen, compatibilizer, and film enhancer constituting the triple helix collagen film of the present invention are all water-soluble polymers. Cellulose nanofibers are water-dispersible polymers. Therefore, in order to produce the triple spiral collagen film of the present invention, an aqueous solution containing these polymers (a dispersion liquid when cellulose nanofibers are contained) is prepared, and the solution is cast on an appropriate release sheet. It may be dried at a temperature of 30 ° C. or lower. Drying may be performed under reduced pressure. The finished film on the release sheet can be punched out or cut out into an appropriate shape together with the release sheet to complete a triple spiral collagen film.
Alternatively, the material polymer aqueous solution may be poured into the recess of the release film formed into an appropriate shape and dried to obtain triple helix collagen.

First, the triple-helix collagen film of the present invention is placed on a position with fine wrinkles on the skin, and the skin of the film portion is thoroughly massaged with water. By massage, the triple spiral collagen film dissolves to form a thin film on the skin surface, moisturizes the skin and makes the texture of the skin finer, and the wrinkled skin becomes smooth skin. For example, in order to remove fine wrinkles at the outer corners of the eyes, the skin at the outer corners of the eyes is lightly massaged with a lotion, and the triple helix collagen film of the present invention is applied to the site. In that state, by taking an aqueous solution such as a lotion on the palm and rubbing it on the skin of the film-attached portion, the triple-spiral collagen film is rapidly dissolved and absorbed by the wrinkled part of the skin to exert its effect. In principle, any aqueous solution such as a lotion used for massage may be used as long as it contains water, but it is preferable to use a lotion.
The triple helix collagen film can have any shape such as a circular shape, an elliptical shape, or a magatama shape having an appropriate size. The film thickness is preferably 100 μm or less. If the thickness of the triple helix collagen film exceeds 100 μm, it may be difficult to dissolve it quickly when water is added at the time of application. More preferably, it is a triple helix collagen film having a film thickness of 5 to 50 μm. If the film thickness is less than 5 μm, the mechanical strength of the film is too small and it may be inconvenient to handle.

Although it has already been known that collagen is an effective cosmetic product for moisturizing and anti-wrinkling the skin, the present invention provides a triple helix collagen film, which makes it easier and safer to use collagen than conventional products. It became possible to supply to. Forming collagen into a film is possible for the first time by the present invention. The film form is convenient for sticking to the surface of the skin.

The triple spiral collagen film of the present invention is easily dissolved in water, and when dissolved, it is absorbed through the skin, and is therefore suitable for cosmetic applications such as wrinkle removal.

Hereinafter, embodiments of the present invention will be described based on examples. However, the present invention is not limited to the contents of the examples.

(Manufacturing of film)
Constituent materials such as compatibilizers and film enhancers excluding triple helix collagen were weighed, 5 mL of ion-exchanged water was added, and the mixture was heated and stirred to dissolve. After returning this solution to room temperature, 100 mg of triple helix collagen was added and dissolved at room temperature. The obtained aqueous solution was cast on a polyester mold release sheet so that the thickness after drying was about 20 μm, dried at room temperature (25 ° C) for 24 hours, and punched into a circle with a diameter of 1.5 cm to form a triple helix. A collagen film was obtained.
Table 1 shows the addition amount (parts by mass) of the constituent materials of the films of each Example and Comparative Example.

As the triple helix collagen, triple helix collagen Ti-03B (Taki Chemical Co., Ltd.) was used. Dextran 70 (Meito Sangyo Co., Ltd.) was used as the dextran. As hydroxypropyl cellulose (HPC), HPC-H (molecular weight 910,000) and HPC-M (molecular weight 620,000) (Nippon Soda Co., Ltd.) were used. As hyaluronic acid, hyaluronic acid FCH-SU (Kikkoman Biochemifa Co., Ltd.) was used. JP-05S (Japan Vam & Poval Co., Ltd.) was used as polyvinyl alcohol. A reagent (Nacalai Tesque Co., Ltd.) was used for starch, carboxymethyl cellulose (CMC), glycerin, and N-methylpyrrolidone (N-MP). Leocrysta C-2SP (Daiichi Kogyo Seiyaku Co., Ltd.) was used as the cellulose nanofiber.

The triple helix collagen film on the release sheet was punched into a circular patch having a diameter of 1.5 cm at room temperature to obtain a sample film. The sample film was placed on the back of the hand, two drops of water were dropped, and the film was rubbed well with a finger to observe the behavior of the film dissolving in water and integrating with the skin.

(Evaluation of physical properties)
The evaluation items and evaluation scales are as follows. ○ and △ were accepted.
Aqueous solution homogeneity:
○ It becomes a uniform transparent aqueous solution × A film forming ability that causes non-uniform precipitation and does not become a uniform aqueous solution:
○ It becomes a tough film △ It becomes a uniform film, but it is a little brittle
× The appearance of the film, which is in the form of a film but is weak in strength and impractical,:
○ It becomes a transparent or translucent uniform film △ It becomes a translucent and slightly whitish film × It becomes a film with a white discontinuity Water solubility of the film (determined by adding 2 drops of water and rubbing it with a finger):
○ Dissolves quickly (within 10 seconds) and stretches on the skin △ Dissolves (10 to 30 seconds) and stretches on the skin × “Dama” is formed and stretches poorly on the skin

(Wrinkle removal efficacy evaluation)
The method for evaluating the wrinkle removing effect of the triple helix collagen film was as follows. After wetting the outer corners of both eyes of an adult male volunteer with a cosmetic solution and applying the collagen film (circle with a diameter of 3 cm) produced in Example 4, the collagen film dissolved by adding water was massaged and applied to the entire outer corners of the eyes. The wrinkle area was measured by the three-dimensional skin unevenness measurement method using PRIMOS Lite manufactured by GFM of Germany before, second, fifth and sixth times of use. Table 2 summarizes the relative wrinkle areas associated with the use of the triple helix collagen film, with the pre-use as 100. It was observed that the use of the triple helix collagen film surely reduced the skin wrinkles.

After attaching the triple helix collagen film produced in Example 5 to both outer corners of another adult male volunteer, the collagen film dissolved by adding water was applied to the entire outer corners of the eyes. The wrinkle area was measured by a three-dimensional measurement method using PRIMOS Lite manufactured by GFM of Germany before and after the sixth use. Table 3 summarizes the relative wrinkle area associated with use, assuming that the wrinkle area before use is 100. It can be seen that wrinkles were reduced by the effect of the collagen film.

Claims (8)

A triple-helix collagen film used as a cosmetic product, which contains triple-helix collagen and a compatibilizer as a material.
The compatibilizer is at least one selected from the group consisting of dextran and starch.
A triple helix collagen film used as a cosmetic product , wherein the compatibilizer is 3 to 90 parts by mass with respect to 100 parts by mass of the triple helix collagen. The triple helix collagen film used as a cosmetic product according to claim 1, further comprising a film enhancer as a material. The triple helix collagen film used as a cosmetic product according to claim 2, wherein the film enhancer is cellulose nanofibers. The triple helix collagen film used as a cosmetic product according to claim 2 or 3, wherein the film enhancer is contained in an amount of 0.1 to 40 parts by mass with respect to 100 parts by mass of the triple helix collagen. The triple helix collagen film used as a cosmetic product according to any one of claims 1 to 4, further comprising a water-soluble plasticizer as a material. The triple helix collagen film used as a cosmetic product according to any one of claims 1 to 5, which has a thickness of 100 μm or less. A cosmetic method (excluding medical practice) characterized by applying a triple helix collagen film used as a cosmetic product according to any one of claims 1 to 6 to the skin to reduce or remove wrinkles. A cosmetic method (excluding medical practice) characterized in that a triple spiral collagen film used as the cosmetic product according to any one of claims 1 to 6 is applied to the skin and then dissolved by adding water or cosmetic water. ..