JP6159957B2 - Inspection equipment - Google Patents

Description

  The present invention relates to a testing apparatus used for testing a specimen such as a nasal cavity or pharyngeal swab, nasal discharge, sputum, urine, serum, stool or rectal swab. Specifically, the present invention relates to an inspection apparatus that can prevent infection and contamination by a specimen and can easily inspect the specimen.

  In recent years, many clinical tests using immunochromatography have been adopted in clinical tests for diagnosing the health condition of patients. The immunochromatography method (1) requires no special measuring machine and can be judged visually. (2) The time required for the inspection is only a few minutes. (3) The operation for the inspection is simple. It has excellent features such as requiring no special technology. Due to these characteristics, the immunochromatography method is mainly used for virus inspection such as influenza and bacterial infection inspection in the medical field, and enables rapid treatment start and accurate treatment after inspection diagnosis.

  Virus inspection and bacterial infection inspection by immunochromatography are mainly performed on specimens collected by wiping the patient's nasal cavity, pharynx or conjunctiva with a specimen collection tool such as a cotton swab or swab. The collected specimen is dispersed or dissolved by being immersed in the extract, and is prepared as a developing solution for immunochromatography measurement. As shown in FIG. 11, a predetermined amount of the extract 5 ′ in which the specimen is dispersed is dropped from an addition hole 33 ′ provided on the surface of a housing 32 ′ in which an immunochromatographic test strip 31 ′ is incorporated. Is introduced into the test strip 31 '. At that time, if the specimen contains highly infectious virus or bacteria, the specimen extract and the used specimen collection tool will be in contact with the handler, or the specimen extract will be exposed to the external environment. There is a risk of microbial contamination due to splattering or leakage, contact of the sample collection tool with the external environment, etc. Therefore, it is necessary to take measures to prevent infection and microbial contamination not only during the examination but also until the specimen collection tool and specimen extract after the examination are discarded.

  Therefore, the present applicant has proposed a test kit that can prevent contact with the handler and leakage to the external environment (Patent Document 1 and Patent Document 2).

  The test kit proposed in Patent Document 1 includes a sample collection jig, a container body in which a sample extract is accommodated, and an opening in the container body while the sample collection jig is accommodated in the container body. And a sealing lid with a specimen collecting jig accommodation function. Therefore, in this test kit, after the sample collected by the sample collecting jig is transferred to the sample extract, the sample extract is placed in the space formed by the container body and the sealing lid with the sample collecting jig accommodating function. And used specimen collection jigs can be sealed.

  In addition, the test kit proposed in Patent Document 2 includes a sample collecting unit at one end in the longitudinal direction, a cap unit at the other end in the longitudinal direction, and a sample collecting tool including a shaft portion therebetween, and an adjustment liquid Is mainly provided with a sample adjustment container that can store the sample collection part and the shaft part of the sample collection tool. This test kit collects the sample by holding the cap part of the sample collection tool, and after transferring the sample to the adjustment liquid, accommodates the sample collection part and the shaft part of the sample collection tool in the sample adjustment container, By sealing the opening of the sample adjustment container as it is with the gripping cap, the sample adjustment liquid and the used sample collection tool can be sealed in the sample adjustment container.

Japanese Patent No. 4801030 JP 2013-228235 A

  In any of the test kits described in Patent Documents 1 and 2, sample preparation (extraction) is performed in a sealed manner, and a closed system can be realized. However, as shown in FIG. 11, when the adjusted extract 5 ′ is applied to the inspection means 3 ′ such as the test strip 31 ′, the extract 5 ′ is “dropped” from the sealed extraction container 2 ′. Therefore, it is in an unsealed (open) state, and further closed system construction has been demanded.

  Further, when the adjusted extract is applied to an inspection means such as a test strip, the extract is, for example, “3 drops” or “5 drops” from an addition hole provided on the surface of the housing in which the test strip is accommodated. ”And so on, because it had to be dripped while counting a predetermined number of times. In addition, since the addition hole provided on the surface of the housing is usually as small as several millimeters in length and width, the extraction liquid does not drop well, it spills to another place, and it is difficult to reliably drop a predetermined amount There was a thing.

  An object of the present invention has been devised in view of the above points, and a test apparatus that can be added and introduced by another method without dropping a specimen sample such as a specimen extract onto a testing means such as a test strip. Is to provide.

  Another object of the present invention is to perform a closed and safe test so that a specimen sample that may contain a virus, bacteria, or the like does not come into contact with the handler or be exposed to the external environment. Therefore, an object of the present invention is to provide an inspection apparatus that can be operated easily.

  The inspection apparatus of the present invention includes inspection means for inspecting an added extract using an immunochromatography method or a nucleic acid chromatography, and an extraction container in which the extract is accommodated. The inspection means is embedded in the addition hole so that one end of the housing is provided with an addition hole for adding the extract, the test strip accommodated in the housing and capable of developing the extract, and one end of the test strip. The extraction container has a capillary action, and the extraction container is configured to be hermetically communicated with the addition hole of the inspection means, and the extraction container is extracted by communicating with the addition hole of the inspection means. The extract liquid absorbed by the liquid derivative moves in the extract liquid derivative by capillary action, and is added to the test strip through one end of the extract liquid derivative in contact with the test strip.

  The inspection apparatus of the present invention includes at least inspection means and an extraction container. Among these, the inspection means has a housing provided with an addition hole and a test strip capable of developing the extract, and the test strip is accommodated inside the housing. An extraction liquid derivative having a capillary action is embedded in the addition hole provided in the housing, and one end of the extraction liquid derivative is disposed in contact with the test strip in the housing. Further, the extraction container is configured to communicate with the addition hole of the inspection means in a sealing manner. Therefore, when the extraction container and the addition hole of the inspection means communicate in a sealing manner, the extract contained in the extraction container is added to the test strip via the extract derivative embedded in the addition hole. The That is, the extract that has flowed into the addition hole is absorbed by the extract derivative having capillary action, and then moves through the extract derivative in the direction of the test strip by capillary action, and is tested through one end that contacts the test strip. Added to the strip. Therefore, the extract can be easily and reliably added to the test strip without dropping the extract.

  The extraction container has a body part, a bottom part in which one end in the axial direction of the body part is sealed, and an opening provided at the other end in the axial direction of the body part. Provided with through-hole forming means for forming a through-hole at the bottom, and configured so that the extraction container and the addition hole of the inspection means are hermetically communicated through the through-hole formed by the through-hole forming means It is also preferable that The communication between the extraction container and the addition hole of the inspection means is performed by breaking and penetrating the bottom of the extraction container with a through-hole forming means provided in the inspection means. At that time, the extraction container and the addition hole of the inspection means are hermetically communicated, and the extraction liquid is absorbed by the extraction liquid derivative embedded in the addition hole from the bottom of the extraction container. Thereby, when it is desired to add the extract to the inspection means, the extraction container and the inspection means can be easily communicated directly. In addition, since the extraction container and the test means are hermetically communicated, the tester can safely test and dispose of the used test equipment without contacting the sample and the instrument to which the sample has adhered. Can do.

  It is also preferable that the through hole forming means is a convex claw portion provided at the peripheral edge portion of the addition hole. In communication between the extraction container and the inspection means, the bottom portion of the extraction container is pierced and penetrated by the through-hole forming means provided in the inspection means, and the through-hole forming means is a convex claw portion provided at the peripheral portion of the addition hole. This convex claw part breaks the bottom part of the extraction container and forms a through hole. At that time, the bottom of the extraction container and the addition hole of the inspection means are hermetically communicated, and the extract moves from the extraction container to the addition hole of the inspection means and is absorbed by the extract derivative embedded in the addition hole. By fitting the convex claw provided at the peripheral edge of the addition hole to the bottom of the extraction container in this way, a through-hole can be formed in the bottom so that the extraction container and the inspection means can communicate with each other. An inspection device can be used.

  The through hole forming means is preferably a pointed end formed by sharpening the other end of the extract derivative. In communication between the extraction container and the inspection means, the bottom portion of the extraction container is broken by the through-hole forming means provided in the inspection means, and the through-hole forming means is formed by sharpening the other end of the extract derivative. It is a pointed end, and the pointed end breaks the bottom of the extraction container to form a through hole. At that time, the bottom of the extraction container and the addition hole of the inspection means are hermetically communicated, and the extract moves from the extraction container to the addition hole of the inspection means and is absorbed by the extract derivative embedded in the addition hole. The extraction liquid derivative has a function of absorbing the extraction liquid and guiding the extraction liquid to the test strip, and a function of forming a through hole in the extraction container and communicating the extraction container and the inspection means. Thus, since the through hole forming means can have a simple configuration, the structure of the inspection means can be simplified.

  The bottom portion of the extraction container is provided with at least one bottom side engaging means disposed on the inner wall or the outer wall thereof, and the outer wall or the inner wall in the vicinity of the adding hole has an addition hole engaged with the bottom side engaging means. It is preferable that side engagement means is provided. The inspection apparatus of the present invention is configured so that the extract contained in the extraction container can be added to the test strip via the extract derivative provided in the inspection means. During this addition, the inner wall or outer wall near the bottom of the extraction container engages with the outer wall or inner wall near the addition hole of the inspection means, so that the bottom of the extraction container and the addition hole of the inspection means are securely fixed. It becomes. Therefore, it is not necessary for the operator to fix the extraction container or the inspection means by hand, the extraction liquid is absorbed and moved through the extraction liquid derivative from the extraction container, and the extraction liquid is easily and reliably inspected. It is possible to move to the test strip of the means. In addition, since the addition hole of the housing is closed by the bottom of the extraction container, the tester can safely test and dispose of the used test equipment without contacting the sample and the instrument to which the sample has adhered. Can do. Specifically, the examination in the examination room or ward where the specimen is collected is efficient and can be performed easily and safely, as well as at school or at home, and it is also used as an OTC examination device. obtain. In addition, since the inspection can be performed in a closed manner, contamination from the external environment can be effectively prevented, particularly when the inspection using the nucleic acid chromatography method is performed.

  It is also preferable that the extract derivative is supported and fixed by the extract derivative support means provided inside the addition hole of the inspection means. Thereby, the extraction liquid derivative can be prevented from coming off from the addition hole. In addition, the extract derivative embedded in the addition hole of the inspection means can maintain the state where one end is in contact with the test strip, and the extract moved from the extraction container can be suitably and quickly moved to the test strip. It is fixed as follows. Thereby, when the extraction container and the inspection means communicate with each other, the extract can be easily and reliably moved to the test strip.

  It is also preferable that the extraction container is made of a non-flexible material having a small fluid permeability. In the conventional inspection apparatus, as shown in FIG. 11, the extraction container 2 ′ is soft and flexible in order to press the body of the extraction container 2 ′ containing the extraction liquid and drip the extraction liquid 5 ′. It was formed with a functional resin. However, since the soft flexible resin has a low density, the water in the extraction liquid contained in the extraction container becomes water vapor and passes through the wall of the extraction container. There was a problem of doing so. However, in the present invention, it is not necessary to press the extraction container and drop the extraction liquid onto the inspection means, and the extraction liquid is applied to the test strip of the inspection means by the capillary action of the extract derivative embedded in the addition hole of the inspection means. Added. Therefore, the extraction container does not need to be formed of a flexible material so that it can be pressed. Therefore, the extraction container can be formed of a non-flexible material having a small fluid permeability, and the extraction liquid can be prevented from permeating out of the extraction container and decreasing. Accordingly, the inspection apparatus can be stored for a long time without being enclosed in a package having a high gas barrier property.

  The extract derivative is also preferably a fiber bundle structure or a sintered porous body. A suitable material is selected as the extract derivative that directs the extract to the test strip of the test means and moves it to the test strip.

According to the present invention, it is possible to provide an inspection apparatus having the following excellent effects.
(1) The extract can be added to the test strip via the extract derivative. Without dripping the extract, the extract can be easily and reliably introduced to the test strip.
(2) When the extraction liquid in the extraction container is to be added to the inspection means, the inspection can be performed by easily communicating the extraction container and the addition hole of the housing.
(3) Since the extract can be added to the test means in a closed manner, the specimen can be safely inspected without the operator touching the specimen and the instrument to which the specimen has adhered, and the used testing equipment can be safely Can be disposed of. Further, contamination from the external environment can be effectively prevented.
(4) Since it is not necessary to drop the extraction liquid onto the inspection means, the extraction container can be formed of a non-flexible or rigid material with low fluid permeability, and the extraction liquid permeates out of the extraction container and is extracted. Can be prevented from decreasing. As a result, the inspection apparatus can be stored for a long period of time.

It is sectional drawing of the extraction container and test | inspection means of the apparatus for a test | inspection which concern on 1st embodiment of this invention, and the front view of a sample collection tool. It is explanatory drawing which shows the place which has added the extract to the test strip of a test | inspection means using the apparatus for a test | inspection of FIG. It is an expanded-axis sectional view of the extraction container in the inspection apparatus shown in FIG. 5A is an enlarged cross-sectional view of the inspection means and FIG. 4B is a partially exploded view of FIG. 4A in the inspection apparatus shown in FIG. 1. It is explanatory drawing which shows roughly the use condition of the apparatus for a test | inspection shown in FIG. 1, Comprising: (a) The state of the extraction container and test | inspection means before use, (b) In the accommodation area | region of the bottom part of an extraction container, A state in which the vicinity of the addition hole is accommodated, (c) a state in which the sealing portion at the bottom of the extraction container is broken by the convex claw provided at the peripheral edge of the addition hole, and the extraction container and the inspection means are in communication with each other Each is shown. (A) Partial expansion axis sectional drawing of an extraction container in the inspection apparatus which concerns on 2nd embodiment of this invention, (b) It is the sectional view on the AA line of Fig.6 (a). (A) The partial expanded sectional view of the test | inspection means in the apparatus for an inspection which concerns on 2nd embodiment, (b) It is an exploded view of Fig.4 (a). It is explanatory drawing which shows roughly the use condition of the apparatus for a test | inspection which concerns on 2nd embodiment of this invention, Comprising: (a) The state of the extraction container and test | inspection means before use, (b) Accommodation of the bottom part of an extraction container A state where the vicinity of the addition hole of the inspection means is to be accommodated in the region; (c) the sealing portion at the bottom of the extraction container is torn at the tip of the extract derivative provided in the inspection means, the extraction container and the inspection means; Each of the states in which is communicated. (A) Partial expanded axial sectional view of an extraction container in the inspection apparatus according to the third embodiment of the present invention, (b) Partial expanded sectional view of the inspection means. It is explanatory drawing which shows roughly the use condition of the apparatus for test | inspection which concerns on 3rd embodiment of this invention, Comprising: (a) The state of the extraction container and test | inspection means before use, (b) Accommodation of the bottom part of an extraction container A state in which the vicinity of the addition hole of the inspection means is screwed into the region, and (c) the sealing portion at the bottom of the extraction container is broken at the tip of the extract derivative provided in the inspection means, A state in which the inspection means is communicated is shown. It is explanatory drawing which shows the use condition of the conventional inspection apparatus.

  Hereinafter, a first embodiment of the present invention will be described with reference to FIGS.

  As shown in FIG. 1, the testing apparatus 1 according to the first embodiment of the present invention includes an extraction container 2, a testing means 3, and a sample collecting tool 4. The inspection apparatus 1 according to the present embodiment shows a lateral flow type inspection apparatus using an immunochromatography method used for inspection of influenza virus as an example, but the inspection apparatus of the present invention includes a nucleic acid chromatography method. The inspection equipment used is also included. Examples of test targets include, for example, influenza viruses, RS viruses, group A β-streptococci, adenoviruses, noroviruses, rotaviruses, sapoviruses, pneumonia mycoplasmas, etc., microorganisms / plants / proteins derived from non-human animals, microorganisms / plants・ Non-human animal-derived nucleic acids, human-derived proteins such as hemoglobin and transferrin, human-derived nucleic acids, tumor markers, hormones, vitamins, bioactive amines, prostaglandins, antibiotics, allergens, pesticides, etc. Is mentioned. By performing an inspection using the inspection apparatus 1, the extraction liquid 5 can be simply and reliably applied to the test strip 31 of the inspection means 3 without dropping the extraction liquid 5 as shown in FIG. 2. Addition can be introduced.

  First, the extraction container 2 will be described with reference to FIGS. The extraction container 2 of the present embodiment is a cylindrical container in which an extraction liquid 5 for dispersing or dissolving a specimen is accommodated, and the body part 21 and one end side in the axial direction of the body part 21 are sealed. It has a bottom 22 and an opening 23 having an opening at the other end. The opening 23 is provided with a lid 24 in advance for preventing the stored extract 5 from flowing out and foreign matters from entering. The lid 24 is configured to be removable for inserting the sample collection tool 4 and can seal the opening 23 of the extraction container 2. Specifically, as the lid 24, a film seal lid that covers the opening 23 by attaching a resin film to the end face of the opening 23 by an adhesive, heat seal, ultrasonic welding or the like, a screw-type lid, a push-in plug Etc. are used. By removing the lid 24, the sealed state of the extraction container 2 is once released, and the sample collection part 41 and the shaft part 42 of the sample collection tool 4 after the sample collection can be inserted into the container. A convex opening side sealing portion 23a that engages with a cap sealing portion 44a of the sample collection tool 4 to be described later and seals the opening portion 23 on the inner wall 21a in the vicinity of the opening portion 23 of the extraction container 2. It has. In the inspection using the nucleic acid chromatography method, the sample collection tool 4 is not used, a nucleic acid solution such as a PCR product is added to the extract 5 as a sample, and the opening 23 is sealed with a cap or the like.

  On the other hand, the bottom 22 of the extraction container 2 is provided with a sealing portion 22a at a position on the opening 23 side that is a predetermined distance away from the end 22d on the bottom side. The sealing portion 22a is provided with a convex claw portion 33c so that a through-hole can be easily formed by a convex claw portion 33c provided at a peripheral portion of an addition hole 33 of the inspection means 3 described later. The contact portion, in this embodiment, has a circumferential break portion 22e in which only the peripheral portion of the sealing portion 22a is thinly formed. Between the sealing part 22a and the end part 22d of the bottom part 22, there is an accommodation area 22b for accommodating the vicinity of the addition hole 33 of the inspection means 3. In the present embodiment, the accommodation region 22 b hermetically accommodates the addition hole 33 together with the addition hole wall 33 a protruding substantially perpendicularly outward in the circumferential direction of the addition hole 33. The inner wall 21a of the accommodating region 22b is provided with a bottom side engaging portion 22c that engages with the outer wall of the addition hole wall 33a of the inspection means 3. In the present embodiment, a convex portion that is continuous along the circumferential direction is provided on the inner wall of the bottom portion 22 as the bottom portion side engaging portion 22c. The bottom side engaging portion 22c may have any structure as long as it can be engaged with the addition hole wall 33a of the inspection means 3. Specifically, for example, in the inner wall 21a or the outer wall 21b of the bottom portion 22, one or a plurality of convex portions, concave portions, convex grooves, concave grooves or ribs that are annularly continuous along the circumferential direction, and spiral in the circumferential direction. The structure which consists of a single or several convex part, a recessed part, a convex groove | channel, a concave groove | channel or a rib which continues in a shape, or these arbitrary combinations is mentioned.

  The extraction container 2 is preferably formed of a non-flexible or rigid material with low fluid permeability, and specifically, although not particularly limited, an olefin-based thermoplastic elastomer, polypropylene resin, high-density polyethylene resin or Glass or the like is preferably used, and polypropylene resin or non-crosslinked olefinic thermoplastic elastomer is particularly preferable. In addition, when forming the extraction container 2 with glass, like this embodiment, about the form which forms a through-hole in the sealing part 22a of the bottom part 22 at the time of use, a through-hole can be formed in the sealing part 22a Thus, it is preferable to form only the sealing portion 22a with a metal thin film such as aluminum, a resin, or the like. The extraction container 2 of this embodiment is formed by integral molding using a non-crosslinked olefin-based thermoplastic elastomer as a non-flexible material. In the inspection apparatus of the present invention, it is not necessary to press the extraction container 2 and drop the extract 5 onto the inspection means 3, and the extract 5 is obtained by the capillary action of the extract derivative 34 provided in the inspection means 3 described later. It is added to the test strip 31 of the inspection means 3. Therefore, it is not necessary to form the extraction container 2 with a pressable flexible material, and the extraction container 2 can be formed with a non-flexible or rigid material with low fluid permeability. 2 to prevent the extract 5 from being permeated to the outside. Accordingly, the inspection apparatus 1 can be stored for a long period of time without being enclosed in a package having a high gas barrier property. The extraction container 2 may be formed of a flexible resin material. Further, in the extraction container 2, it is visually determined whether or not the convex claw portion 33c of the inspection means 3 breaks through the sealing portion 22a of the extraction container 2 and the extraction container 2 and the inspection means 3 are in communication with each other. It is also preferable to form a transparent or translucent material so that it can be confirmed.

  Next, the inspection means 3 will be described with reference to FIGS. The inspection means 3 of the present embodiment is schematically configured from a test strip 31 on which the extract 5 is developed and a test result is displayed, and a housing 32 that houses the test strip 31. A substantially circular addition hole 33 for adding the extract 5 is provided on the surface of the housing 32, and a determination window 35 for displaying the inspection result is further provided. The addition hole 33 has a cylindrical addition hole wall 33a formed so as to protrude substantially perpendicularly outward in the circumferential direction of the hole, and when the extraction container 2 and the inspection means 3 communicate with each other, the extraction container 2 The extraction container 2 that is engaged with and communicated with the bottom portion 22 side is supported and fixed, and leakage of the added extract 5 can be prevented. In the present embodiment, the storage region 22b of the bottom 22 of the extraction container 2 is configured to be able to store the addition hole 33 together with the addition hole wall 33a, and the convex bottom side provided on the inner wall of the storage region 22b. The engaging portion 22c presses and engages the outer wall of the addition hole wall 33a to maintain the connection state between them, and seals the space between the bottom portion 22 of the extraction container 2 and the addition hole 33 of the inspection means 3. Thus, the leakage of the extract 5 can be suitably prevented. In the present embodiment, in order to further improve the sealing performance, when the extraction container 2 and the inspection means 3 communicate with each other, the end 22d of the bottom 22 of the extraction container 2 abuts on the surface of the housing 32 of the inspection means 3. Thus, the length from the sealing portion 22a to the end portion 22d of the bottom portion 22 and the height of the wall of the addition hole wall 33a are designed.

  In addition, a convex claw portion 33c is disposed at the peripheral portion of the addition hole 33 described above, that is, at the upper end portion of the addition hole wall 33a. By inserting the vicinity of the addition hole 33 of the inspection means 3 into the accommodation region 22b of the bottom portion 22 of the extraction container 2, the protruding claw portion 33c presses the fracture portion 22e of the sealing portion 22a of the extraction container 2, and the through hole 25 And the extraction container 2 and the inspection means 3 communicate with each other. In order to more reliably form the through hole 25, the convex claw portion 33c includes a thin portion on the distal end side and a thick portion on the proximal end side (addition hole wall 33a side), and the thin portion on the distal end side. It is also preferable that a step is formed between the base portion and the thick portion on the base end side. In the case of such a configuration, the level difference of the convex claw portion 33c abuts on the sealing portion 22a of the extraction container 2 and causes deformation of the fractured portion 22e. A gap is likely to be formed in the vicinity of the through hole 25, and the extract 5 quickly moves to the addition hole 33 of the inspection means 3 through this gap.

  An extraction liquid derivative 34 is embedded in the addition hole 33, and in this embodiment, a rod-shaped extraction liquid derivative 34 is embedded in a space inside the addition hole wall 33a. The extraction liquid derivative 34 is formed in a substantially cylindrical shape in the present embodiment, but can be formed in various shapes such as an elliptical column shape and a polygonal column shape. Further, the shape of the axial cross section may not be constant. One end of the extract liquid derivative 34 constitutes a contact part 34a arranged so as to contact the test strip 31, and the extract liquid 5 absorbed by the extract liquid derivative 34 through the contact part 34a is the test strip. Expanded to 31. The extract liquid derivative 34 is locked by an extract liquid derivative support portion 33b provided inside the addition hole wall 33a of the addition hole 33 to prevent the extract liquid derivative 34 from dropping from the addition hole 33 and to extract the liquid extract derivative. One end (contact portion 34 a) of 34 is configured to keep in contact with the test strip 31. As shown in FIG. 4 (b), in this embodiment, the extract derivative support portion 33b is formed in a convex shape provided at four locations at substantially equal intervals along the circumferential direction on the inner wall of the addition hole wall 33a. In addition, the inner diameter of the addition hole 33 is slightly narrow in the portion of the extract derivative support portion 33b.

  In this embodiment, the extract liquid derivative 34 is formed in an elongated cylindrical shape, and the extract liquid 5 flowing into the addition hole 33 is absorbed by the extract liquid derivative 34 and passes through the contact portion 34 a of the extract liquid derivative 34. It is configured to move to the test strip 31. Since the extract 5 is added to the test strip 31 by utilizing the capillary action of the extract derivative 34, the extract derivative 34 absorbs and holds the extract 5 in the test strip 31 according to the development state of the extract 5 in the test strip 31. The extracted liquid 5 is added to the test strip 31. Therefore, in the conventional addition by dripping, a certain amount or more of the extract is added to the test strip at a time, and the extract is not absorbed by the test strip, and the extract may leak out. According to the addition through the extract derivative 34, such leakage can be prevented. The shape of the extract liquid derivative 34 is not particularly limited, and can be appropriately adjusted according to the shape of the addition hole 33 to be embedded. In addition, as will be described later, the shape of the end portion can be changed.

  In the present embodiment, the shape of the end portion of the extract liquid derivative 34 on the contact portion 34a side is such that the extract solution 5 moves to the test strip 31 of the inspection means 3 via the contact portion 34a. 5 is formed in a substantially flat shape having a large contact area. However, any shape may be used as long as the extract 5 can be moved to the inspection means 3. In addition, regarding the shape of the other end, any shape can be selected in the present embodiment.

  As the extract liquid derivative 34, any one having a capillary action may be used. For example, a fiber bundle structure, a sintered porous body, paper, a sponge, a nonwoven fabric, a woven fabric, or the like can be used. In the present invention, a fiber bundle structure or a sintered porous body is suitably used from the viewpoint of excellent capillary action. The fiber bundle structure is a structure formed by focusing and bonding sliver or filament such as synthetic resin fiber, pulp fiber, glass fiber, etc. with heat or other resin, and from one end of each fiber constituting the fiber bundle. It has a capillary action to move the liquid to the other end side. As the synthetic resin fiber, a fiber made of polyamide, acrylic, rayon, acetate, polyester, polyvinyl chloride, polyethylene, polypropylene, or the like is preferably selected. As the extract liquid derivative 34 of the present embodiment, a fiber bundle structure formed by combining fiber bundles mainly made of polyester fibers with a polyurethane resin is selected. The fiber thickness of the fiber bundle structure is preferably 1 to 10 denier, more preferably 1 to 7 denier, and particularly preferably 2 to 5 denier from the viewpoint of capillary action. In addition, the porosity of the fiber bundle structure is preferably 35 to 80%, more preferably 40 to 70%, and particularly preferably 45 to 65% from the viewpoints of capillary action, absorbability described later, and filtration performance. On the other hand, a sintered porous body is a porous body formed by sintering particles such as resin or metal, ceramic powder, or metal fiber in a three-dimensionally entangled state, and is liquid via a three-dimensional network structure. It has a capillary action to move. As the particle raw material, a resin is preferable, and polyethylene (low density polyethylene, high density polyethylene, ultrahigh molecular weight polyethylene), polypropylene, polystyrene, polymethyl methacrylate, and the like are suitably selected. The pore diameter of the sintered porous body is preferably 10 to 200 μm, more preferably 20 to 100 μm, from the viewpoint of capillary action. The extract derivative 34 such as a fiber bundle structure or a sintered porous body may be treated with a surfactant or the like in order to enhance hydrophilicity and absorbability. The extract liquid derivative 34 such as the above-described fiber bundle structure or sintered porous body has a capillary action and a high absorbability. Therefore, the extract 5 flowing in from the extraction container 2 can be quickly absorbed and quickly guided to the test strip 31 of the inspection means 3 by capillary action. In addition, since the extract liquid derivative 34 such as a fiber bundle structure has a high liquid holding property, the extract liquid 5 is extracted after the extract liquid 5 is added through the extract liquid derivative 34 so that the test strip 31 can absorb. Since the extract 5 is not further added to the test strip 31 from the contact portion 34a of the liquid derivative 34, the extract 5 does not leak from the test strip 31, and the inspection can be performed safely. Furthermore, the extract liquid derivative 34 such as a fiber bundle structure or a sintered porous body also has filtration performance due to its structure, and a highly viscous secretion derived from the collected specimen contained in the extract 5 It also has the effect of removing unnecessary substances such as cell debris. Therefore, since it is not necessary to perform filtration using a filtration instrument or the like separately prepared for the extract 5, the configuration of the inspection device can be simplified.

  Further, in this embodiment, the test strip 31 is configured as a lateral flow type test strip using an immunochromatography method, and includes a sample pad 31 a that absorbs the added extract 5, a labeled antibody, and the like. It has a conjugate pad 31b, a membrane 31c containing a supplementary antibody or the like and displaying a test result, and an absorption pad 31d that absorbs excess extract. When the inspection means 3 is the inspection means 3 using a nucleic acid chromatography method, the test strip 31 may be selected for nucleic acid chromatography. The test strip 31 using the nucleic acid chromatography method includes, as an example, a sample pad 31a that absorbs the added extract 5, a conjugate pad 31b that contains a labeling substance such as latex particles, a supplementary probe, and the like. It comprises a membrane 31c on which the inspection result is displayed, and an absorption pad 31d that absorbs excess extract. The extract 5 introduced from the addition hole 33 through the extract derivative 34 is first introduced into the sample pad 31a by capillary action, and then developed into the conjugate pad 31b, the membrane 31c, and the absorption pad 31d by capillary action. The The inspection result displayed on the membrane 31c can be confirmed through the determination window 35. The test strip 31 is not limited to the above-described configuration, and widely includes a flow-through type test strip and other combinations as appropriate. Further, the test strip 31 can be designed so as to absorb only a certain amount of the extract 5 by adjusting the specifications of the material constituting the test strip 31. Since the extract liquid 34 is not added to the test strip 31 that has absorbed a certain amount of the extract 5 due to its capillary action, no more extract 5 is added. 5 can be added to the test strip 31 via the extract derivative 34.

  In order to avoid contact with the extract 5 added to the inspection means 3 and to close the inspection apparatus 1, the determination window 35 portion of the housing 32 is sealed with a transparent resin film, The portion excluding the addition hole 33 can be shrink-processed with a transparent resin film. In addition, at least a portion of the test strip 31 exposed from the determination window 35 can be laminated with a resin film.

  Next, a sample collection tool 4 for collecting a sample to be examined will be described with reference to FIGS. As shown in FIG. 1, the sample collection tool 4 according to the present embodiment includes a shaft portion 42, a sample collection portion 41 provided integrally with one end in the longitudinal direction of the shaft portion 42, and the other end in the longitudinal direction. It is mainly composed of a cap portion 44 provided integrally, and is inserted through the patient's nostril, mouth, etc., and the sample is removed by wiping the back of the nose and throat with the sample collection portion 41 provided at the tip thereof. It is configured to be able to collect. Among these, the cap portion 44 includes a cap sealing portion 44 a that can be engaged with the opening-side sealing portion 23 a provided on the inner wall near the opening portion 23 of the extraction container 2 to seal the opening portion 23. Yes. As a result, as shown in FIG. 2, the sample collection portion 41 and the shaft portion 42 of the sample collection tool 4 that collected the sample are bent and accommodated inside the extraction container 2, and the opening 23 is sealed with the cap portion 44 as it is. Thus, the extraction container 2 can be sealed. The sample collection tool 4 may be configured only of the sample collection unit 41 and the shaft unit 42 without including the cap unit 44. In addition, depending on the inspection object, the length of the shaft portion 42 may be shortened so that it can be accommodated in the extraction container 2 without being bent.

  As shown in FIG. 1, in this embodiment, the shaft part 42 of the sample collecting tool 4 is composed of a flexible part 42a and a base part 42b. The flexible part 42a is a small-diameter part from a predetermined position to the specimen collecting part 41, and has flexibility to be deformed by applying stress. The base part 42b is a thick part from the boundary part with the flexible part 42a to the cap part 44, and has higher rigidity than the flexible part 42a. Therefore, as shown in FIG. 2, when the sample collection part 41 and the shaft part 42 of the sample collection tool 4 are accommodated in the extraction container 2, a force is applied to the flexible part 42 a of the shaft part 42. The length of the shaft portion 42 in the accommodated state can be shortened by bending or bending the 42a. At a predetermined position of the base portion 42b of the shaft portion 42, a notch 43 is provided for easily cutting and dividing the base portion 42b in a direction perpendicular to the axial direction. By bending the base part 42b at the position where the notch 43 is provided, the base part 42b is easily cut and divided in the axial direction, so that the length of the shaft part 42 accommodated in the extraction container 2 is further shortened. can do.

  Although it does not specifically limit as a material which comprises the axial part 42, Resin, such as polystyrene, polyethylene, a polypropylene, nylon, or polyester, paper, etc. are used suitably. Moreover, in this embodiment provided with the flexible part 42a in the axial part 42, the material which has the flexibility which deform | transforms by applying stress is selected. Further, the sample collection unit 41 may be any one that can attach a sample, and a cotton ball, a cotton swab equipped with a cotton ball, a sponge, a brush, or the like is appropriately selected. In particular, a cotton swab in which a synthetic fiber is flocked to form a cotton ball is preferably used because of its excellent sample collection function. The sample collected by such a sample collecting tool 4 is not particularly limited, but is a human or non-human animal nasal cavity or throat swab, nasal discharge, sputum, urine, blood, plasma, serum, feces, rectal swab, Conjunctival wipes, saliva, tears, amniotic fluid, biological tissue wipes, cerebrospinal fluid and pus juice, food wipes, food extracts, beverages, tap water, drainage, environmental water, soil and plant extracts It is done. Note that the sample collection tool 4 is not used for the inspection using the nucleic acid chromatography method.

  Next, a method for using the inspection apparatus 1 according to the present embodiment will be described based on FIGS. 1, 2, and 5. In FIG. 5, the sample collection tool 4 is omitted.

  First, a sample is collected using the sample collection tool 4 shown in FIG. 1 (not shown). When collecting a sample, the sample collection unit 41 and the shaft unit 42 can be operated by holding the cap unit 44. The specimen is collected by wiping the patient's nostril, pharynx, conjunctiva, urethra, uterus, vagina, stool, urine, sputum and the like with the specimen collection unit 41. The collected specimen adheres to the specimen collection section 41 and is held.

  Next, the sample collection tool 4 after collecting the sample from the opening 23 of the extraction container 2 is inserted with the sample collection unit 41 from the opening 23 of the extraction container 2 with the cap 44 in place. The collection part 41 and the shaft part 42 are accommodated inside the container. At this time, as shown in FIG. 2, the flexible portion 42 a of the shaft portion 42 is curved and deformed into a loop shape, and the position where the notch 43 of the shaft portion 42 is applied is pressed against the edge of the opening 23. The shaft portion 42 is cut and divided at this position, for example, by bending. Accordingly, the length of the shaft portion 42 and the like of the sample collection tool 4 can be accommodated in the container of the extraction container 2 while being substantially shortened. After the storage, the opening 23 of the extraction container 2 is sealed and sealed with the cap sealing portion 44a of the cap portion 44 as it is.

  Next, as shown in FIG. 5A, the bottom 22 of the extraction container 2 is placed near the addition hole 33 of the inspection means 3 in order to accommodate the addition hole 33 of the inspection means 3 together with the addition hole wall 33a in the accommodation area 22b. Move closer.

  Next, as shown in FIG. 5 (b), the accommodation region 22 b of the extraction container 2 is pushed in and attached so as to cover the addition hole 33 of the inspection means 3. At this stage, the extraction container 2 and the inspection means 3 are not in communication with each other, but are in a temporarily fitted state, and the convex claw portion 33c provided at the upper end of the addition hole 33 of the inspection means 3 is the extraction container. The through hole is not formed because it is not in contact with the second sealing portion 22a.

  Next, as shown in FIG. 5 (c), when a force is further applied in the direction from the extraction container 2 to the inspection means 3, the convex claw portion 33c provided at the upper end of the addition hole 33 of the inspection means 3 becomes The breaking part 22e of the sealing part 22a of the extraction container 2 is pressed and pierced to form a through hole 25 in the sealing part 22a. Thereby, the extraction container 2 and the inspection means 3 communicate with each other, and the extraction liquid 5 accommodated in the extraction container 2 flows into the addition hole 33 of the inspection means through the through hole 25. Further, the end 22d of the extraction container 2 abuts on the surface of the housing 32 of the inspection means 3, and the bottom side engaging portion 22c of the extraction container 2 presses the outer wall of the addition hole wall 33a. 3 is engaged. Thereby, since the extraction container 2 and the test | inspection means 3 are sealingly connected, the leak to the external environment of the extract 5 which flows in into the addition hole 33 can be prevented. The extract 5 flowing into the addition hole 33 of the inspection means 3 is absorbed by the extract derivative 34 embedded in the internal space of the addition hole 33 and moves to the contact portion 34a side of the extract derivative 34 by capillary action. . Since one end of the extraction liquid derivative 34 is in contact with the sample pad 31a of the test strip 31 of the inspection means 3, the extraction liquid 5 moves to the sample pad 31a through the contact part 34a of the extraction liquid derivative 34. As described above, the extract 5 can be introduced into the test strip 31 in a closed manner without dropping the extract 5.

  After the extract 5 is introduced into the inspection means 3, in this embodiment, an inspection by a normal lateral flow method is performed. The inspection result can be confirmed from the determination window 35 of the housing 32. The used inspection device 1 can be disposed of in the closed state shown in FIG. Thereby, the test apparatus 1 can be discarded in a state where the extract 5 containing the specimen does not leak to the outside. As described above, according to the testing apparatus of the present invention, it is possible to efficiently handle and process a sample when testing a sample that may contain a virus, a highly infectious bacterium, or the like. .

  Next, a second embodiment of the present invention will be described with reference to FIGS.

  The inspection apparatus 10 according to the second embodiment of the present invention is different from the inspection apparatus 1 according to the first embodiment in the configuration of the bottom 122 of the extraction container 20, the addition hole 133 of the inspection means 30, and the extract derivative 134. The configuration is substantially the same as in the first embodiment except that is partially different. In the present embodiment, the same components as those in the first embodiment will be described using the same reference numerals.

  Based on FIG. 6, the extraction container 20 of the present embodiment will be described. As shown in FIG. 6 (a), the bottom portion 122 of the extraction container 2 is provided with a sealing portion 122a at a position on the opening side that is a predetermined distance away from the end portion 122d on the bottom side. As shown in FIG. 6B, a through hole 125 can be easily formed in the sealing portion 122a by a pointed portion 134b that is a through hole forming means provided in the inspection means 30 of the present embodiment. In this way, a streak-like fracture portion 122f is provided in which only that portion is thinly formed. In the present embodiment, the fracture portion 122f is formed in the shape of three straight streaks joined at the center of a circle, but is not limited to this mode. Between the sealing part 122a and the end part 122d of the bottom part 122, there is an accommodation area 122b for accommodating the vicinity of the addition hole 133 of the inspection means 30. In the present embodiment, the accommodation region 122b hermetically accommodates the extract liquid derivative 134 and the extract liquid support part 133b embedded in the addition hole 133. In the present embodiment, the outer wall of the accommodation region 122b is provided with a bottom side engaging portion 122c that engages with the inner wall of the addition hole wall 133a of the inspection means 30, and the bottom side engaging portion 122c is the bottom portion. An uneven portion that is continuous along the circumferential direction is provided on the outer wall 122. The bottom side engaging portion 122c may have any structure as long as it can be engaged with the addition hole wall 133a of the inspection means 30. Specifically, for example, in the inner wall 121a or the outer wall 121b of the bottom portion 122, one or a plurality of convex portions, concave portions, convex grooves, concave grooves or ribs that are annularly continuous along the circumferential direction, and spiral in the circumferential direction. The structure which consists of a single or several convex part, a recessed part, a convex groove | channel, a concave groove | channel or a rib which continues in a shape, or these arbitrary combinations is mentioned.

  Based on FIG. 7, the test | inspection means 30 of this embodiment is demonstrated. In the present embodiment, a substantially circular addition hole 133 for adding the extract 5 is provided on the surface of the housing 132 of the inspection means 30. In the present embodiment, unlike the first embodiment described above, the surface of the housing 132 provided with the addition hole 133 is configured to be substantially flat, and the housing 132 is designed to have a large thickness (height). It has the structure which has the addition hole wall 133a, another engaging part, etc. inside. Therefore, in the circumferential direction of the addition hole 133, a cylindrical addition hole wall 133a formed so as to penetrate substantially vertically downward (direction in which the test strip 131 is accommodated) is provided. The addition hole wall 133a is formed to support and fix the bottom 122 of the extraction container 20 and to prevent the extraction liquid 5 from leaking. An addition hole side engagement portion 133d is provided on the inner wall of the addition hole wall 133a to keep the engagement state stable when the bottom portion 122 of the extraction container 20 is inserted into the addition hole 133 and engaged. ing. Specifically, the inner wall of the addition hole wall 133a is continuously provided with an addition hole side engagement portion 133d that is convex and concave in the circumferential direction. This addition hole side engaging part 133d engages with the bottom part side engaging part 122c of the bottom part 122 of the extraction container 20, and fixes and connects both reliably. The addition hole side engagement portion 133d may have any structure as long as it can be engaged with the bottom side engagement portion 122c of the extraction container 20. For example, the addition hole side engagement portion 133d has an annular shape along the circumferential direction on the inner wall of the addition hole 133a. Convex or Convex Convex, Concave, Convex Groove, Concave Groove or Rib, Convex or Convex Convex Convex, Concave, Convex Groove, Concave Groove or Rib Or a structure composed of any combination thereof.

  As shown in FIG. 7, in this embodiment, an extract liquid derivative 134 made of a rod-shaped fiber bundle structure is embedded in the space inside the addition hole 133. In this embodiment, the extract liquid derivative 134 is formed in a substantially cylindrical shape, and one end of the extract liquid derivative 134 is disposed in contact with the test strip 131 to form a contact part 134a. The extract 5 absorbed by the extract derivative 134 through 134 a is developed on the test strip 131. This extract derivative 134 is arranged substantially coaxially inside the cylindrical addition hole wall 133a, and the extract derivative support part 133b is formed of a cylindrical wall surface formed continuously with the addition hole wall 133a at the lower end. It is locked with. Accordingly, the extract liquid derivative 134 is prevented from falling off from the addition hole 133, and one end (the contact part 134a) of the extract liquid derivative 134 is kept in contact with the test strip 131.

  The shape of the end portion of the extract liquid derivative 134 is such that the extract liquid 5 moves to the test strip 131 of the inspection means 30 through the contact portion 134a at the end portion on the contact portion 134a side. In order to increase the movement efficiency, the contact area is formed in a substantially flat shape having a large contact area. However, any shape may be used as long as the extract 5 can be moved to the inspection means 30. On the other hand, the other end portion of the extraction liquid derivative 134 is a pointed end portion 134b with a sharpened tip so that the through hole 125 can be formed by breaking through the sealing portion 122a of the extraction container 20 in this embodiment. It is formed as. The shape of the tip portion 134b is not particularly limited, but can be formed in a weight shape or a taper shape. As shown in the operation explanatory diagram of FIG. 8, when the bottom portion 122 of the extraction container 20 is fitted into the addition hole 133 of the inspection means 30, the pointed portion 134 b of the extraction liquid derivative 134 is brought into contact with the sealing portion 122 a of the extraction container 20. The extraction container 20 and the inspection means 30 are communicated with each other by breaking through the sealing portion 122a.

  The length L134 of the extract liquid derivative 134 is such that one end (the contact part 134a) of the extract liquid derivative 134 comes into contact with the test strip 131 of the inspection means 30 during use, and the pointed part 134b seals the bottom part 122 of the extraction container 20. The length may be such that it can contact and break through the portion 122a, and is preferably longer than the length from the end 122d of the bottom 122 of the extraction container 20 to the sealing portion 122a. In the present embodiment, the length from the end 122d of the bottom 122 of the extraction container 20 to the sealing portion 122a is about 7 mm, and the length L134 of the extraction liquid derivative 134 is about 13 mm. Further, the diameter of the extract liquid derivative 134 is 3.5 mm.

  Further, in the housing 132 of the inspection means 30, the pointed portion 134 b of the extraction liquid derivative 134 embedded in the addition hole 133 pierces the sealing portion 122 a of the extraction container 20, and the extraction container 20 and the inspection means 30 communicate with each other. It is also preferable that it is formed of a transparent or translucent resin so that it can be visually confirmed whether or not. Although not particularly limited, specifically, a synthetic resin such as polyethylene, polypropylene, nylon, polyester, or polystyrene is preferably used.

  The other description about the structure of the extraction container 20 and the test | inspection means 30 is the same as that of the case of 1st embodiment mentioned above, The function and effect are also the same. Further, the configuration of the sample collection tool 4 constituting the test apparatus 10 is the same as that in the first embodiment described above, and the functions and effects thereof are also the same.

  Next, a method of using the inspection apparatus 10 according to the present embodiment will be described based on FIG. In FIG. 8, the specimen collecting tool 4 is omitted.

  When using the test apparatus 10, a sample is collected by the sample collection tool 4, and the sample is suspended in the extract 5 in the extraction container 20, and then, as shown in FIG. Therefore, the bottom 122 of the extraction container 20 is brought close to the addition hole 133 of the inspection means 30 in order to accommodate the extraction liquid derivative 134 formed of the fiber bundle structure embedded in the addition hole 133 in the accommodation region 122b.

  Next, as shown in FIG. 8 (b), the storage region 122 b of the extraction container 20 is pushed in and attached so as to cover the extract liquid derivative 134 provided in the inspection means 30. At this stage, the extraction container 20 and the inspection means 30 are not in communication and are in a temporarily fitted state.

  Next, as shown in FIG. 8 (c), when further force is applied in the direction from the extraction container 20 to the inspection means 30, the tip part 134b of the extract liquid derivative 134 reaches the sealing part 122a of the extraction container 20, The pointed portion 134b breaks the streak-like broken portion 122f of the sealing portion 122a to form the through hole 125. The fiber bundle structure constituting the extract liquid derivative 134 has a certain degree of hardness that can break through the sealing portion 122a, and the through hole 125 can be easily formed. Thereby, the extraction container 20 and the inspection unit 30 communicate with each other, and the extraction liquid 5 accommodated in the extraction container 20 passes through the through hole 125 or the extraction liquid derivative 134 protruding from the through hole 125, and the addition hole 133 of the inspection unit 30. Flow into. Further, the end 122d of the extraction container 20 abuts against a wall surface connecting the addition hole wall 133a and the extraction liquid derivative support part 133b, and the bottom side engaging part 122c of the extraction container 20 and the addition hole side of the inspection means 30 The engaging portion 133d is engaged. Thereby, since the extraction container 20 and the test | inspection means 30 are sealingly connected, the leak to the external environment of the extract 5 which flows into the test | inspection means 30 can be prevented. The other description regarding the usage method of the test | inspection apparatus 10 is the same as that of the case of the usage method mentioned above, The function and effect are also the same.

  Next, a third embodiment of the present invention will be described with reference to FIGS.

  The inspection apparatus 100 according to the third embodiment of the present invention is the same as the extraction container 200 except that the configuration of the bottom side engaging portion 222c of the bottom portion 222 is partially different. A configuration substantially similar to that of the container 20 is provided. The inspection unit 300 has substantially the same configuration as that of the first embodiment except that the configuration of the addition hole 233 and the extraction liquid derivative 234 is partially different. In the present embodiment, the same components as those in the first embodiment will be described using the same reference numerals.

  Based on FIG. 9A, the extraction container 200 of the present embodiment will be described. As shown in FIG. 9A, the bottom portion 222 of the extraction container 200 includes a sealing portion 222a and a fracture portion 222f, an accommodation region 222b, and an end portion 222d similar to the extraction container 20 of the second embodiment. Yes. Also in the present embodiment, the outer wall of the accommodation region 222b is provided with a bottom side engaging portion 222c for engaging with the inspection means 300. However, in this embodiment, the bottom portion 222 is used as the bottom side engaging portion 222c. Concave and convex grooves that are continuous in a spiral shape along the circumferential direction are provided on the outer wall. The bottom side engaging portion 222c is formed so as to be screwable with an adding hole side engaging portion 233d formed of a spiral groove provided on the inner wall of the adding hole wall 233a of the inspection means 300.

  Next, the inspection means 300 of this embodiment will be described based on FIG. As shown in FIG. 9B, the inspection means 300 is provided with an addition hole 233 in the housing 232, and the addition means formed so as to protrude substantially perpendicularly outward in the circumferential direction of the addition hole 233. A hole wall 233a is formed. On the inner wall of the addition hole 233, an addition hole side engagement portion 233d that can be screwed with the bottom side engagement portion 222c of the extraction container 200 described above is provided. Thereby, the extraction container 200 and the test | inspection means 300 can be communicated sealingly by engagement by screwing, and the leakage of the extract 5 at the time of a test | inspection can be prevented.

  In the present embodiment, the extraction liquid derivative 234 embedded in the addition hole 233 includes a pointed portion 234b as in the second embodiment, and the pointed portion 234b breaks through the sealing portion 222a of the extraction container 200. It is configured to be able to. Further, in this embodiment, the extract liquid derivative 234 is formed in an elongated substantially cylindrical shape, but unlike the first and second embodiments, the contact portion 234a side is formed in a large diameter. Yes. Thereby, the extract 5 absorbed in the extract derivative 234 can be added to the test strip 231 more rapidly. In addition, an extraction liquid derivative support part 233b for supporting and fixing the extraction liquid derivative 234 is formed in the addition hole 233 as a convex part continuously protruding in the circumferential direction on the inner wall of the addition hole wall 234a. The addition hole 234 is formed to have a narrow inner diameter. This extract derivative support portion 233b supports and fixes the boundary portion between the large diameter portion on the contact portion 234a side of the extract solution derivative 234 and the small diameter portion above it from the circumferential direction, whereby the extract solution derivative 234 is added. It is configured to prevent the contact portion 234a of the extract liquid derivative 234 from coming into contact with the test strip 231 while preventing it from falling out of the hole 233.

  Next, a method of using the inspection apparatus 100 according to the present embodiment will be described based on FIG. In FIG. 10, the sample collection tool 4 is omitted.

  When using the testing apparatus 100, a sample is collected by the sample collecting tool 4, and the sample is suspended in the extract 5 in the extraction container 200, and then, as shown in FIG. In order to accommodate the extraction liquid derivative 234 embedded in the addition hole 233 in the accommodation region 222 b of the extraction container 200, the bottom 222 of the extraction container 200 is brought close to the addition hole 233 of the inspection means 300.

  Next, as shown in FIG. 10 (b), the body 221 of the extraction container 200 is rotated so that the storage area 222 b of the extraction container 200 is covered with the extraction liquid derivative 234 provided in the inspection means 300. The bottom side engaging portion 222c of 200 and the addition hole side engaging portion 233d of the inspection means 300 are screwed together and attached. At this stage, the extraction container 200 and the inspection means 300 are in a temporarily fitted state, and the pointed portion 234b of the extract derivative 234 embedded in the addition hole 233 of the inspection means 300 is a sealed portion of the extraction container 200. It is not in contact with 222a and no through hole is formed.

  Next, as shown in FIG. 8 (c), the body portion 221 of the extraction container 200 is further rotated so that the bottom side engagement portion 222 c of the extraction container 200 and the addition hole side engagement portion 233 d of the inspection means 300 are screwed. When combined, the pointed end 234b of the extraction liquid derivative 234 reaches the sealing part 222a of the extraction container 200, and the pointed end 234b breaks the fractured part 222f of the sealing part 222a to form a through hole 225. As a result, the extraction container 200 and the inspection unit 300 communicate with each other, and the extraction liquid 5 accommodated in the extraction container 200 passes through the through hole 225 or the extraction liquid derivative 234 protruding from the through hole 225 and the addition hole 233 of the inspection unit 300. Flow into. Further, the end portion 222 d of the extraction container 200 abuts on the upper surface of the addition hole side engagement portion 233 d inside the addition hole 233, and the bottom portion side engagement portion 222 c of the extraction container 200 and the addition hole side engagement of the inspection means 300. Since the coupling portion 233d is engaged by screwing, the extraction container 200 and the inspection unit 300 are hermetically communicated, and leakage of the extract 5 flowing into the inspection unit 300 to the external environment can be prevented. . The other description regarding the usage method of the test | inspection apparatus 100 is the same as that of the case of the usage method mentioned above, The function and effect are also the same.

  The present invention is not limited to the above-described embodiments, and various design changes within the scope not departing from the gist of the invention described in the claims are also included in the technical scope.

1, 10, 100 Inspection device 2, 20, 200, 2 'Extraction container 21, 121, 221 Body 21a Inner wall 21b Outer wall 22, 122, 222 Bottom 22a, 122a, 222a Sealing part 22b, 122b, 222b Storage area 22c, 122c, 222c Bottom side engaging portion 22d, 122d, 222d End portion 22e, 122f, 222f Breaking portion 23 Opening portion 23a Opening portion side sealing portion 24 Lid 25, 125, 225 Through hole 3, 30, 300, 3 'testing means 31, 131, 231 and 31' test strips 31a, 131a and 231a sample pads 31b, 131b and 231b conjugate pads 31c, 131c and 231c membranes 31d absorbent pads 32, 132, 232 and 32 'housings 33 and 133 233, 33 'addition hole 3a, 133a, 233a Additive hole wall 33b, 133b, 233b Extract liquid derivative support part 33c Convex claw part 133d, 233d Additive hole side engaging part 34, 134, 234 Extract liquid derivative 34a, 134a, 234a Contact part 134b, 234b Point L134 Length of extract derivative 35 Judgment window 4 Specimen collection tool 41 Specimen collection part 42 Shaft part 42a Flexible part 42b Base part 43 Notch 44 Cap part 44a Cap sealing part 5, 5 'Extract liquid

Claims (5)

An inspection means for inspecting the added extract using an immunochromatography method or a nucleic acid chromatography, and an extraction container in which the extract is contained,
The inspection means includes a housing provided with an addition hole for adding the extract, a test strip accommodated in the housing and capable of developing the extract, and one end abutting the test strip. And an extract derivative having a capillary action embedded in the addition hole,
The extraction container has a body part, a bottom part in which one end of the body part in the axial direction is sealed, and an opening provided at the other end in the axial direction of the body part,
The extraction container is configured to be able to communicate with the addition hole of the inspection unit in a sealing manner,
The inspection means includes a through hole forming means for forming a through hole in the bottom portion of the extraction container, and the extraction container and the inspection means are connected through the through hole formed by the through hole forming means. The addition hole is configured to communicate with the sealing hole in a sealing manner,
Due to the communication between the extraction container and the addition hole of the inspection means, the extraction liquid absorbed in the extraction liquid derivative moves in the extraction liquid derivative by capillary action and contacts the test strip. Configured to be added to the test strip through the one end of the liquid derivative ;
The inspection device according to claim 1, wherein the through-hole forming means is a convex claw portion provided at a peripheral edge portion of the addition hole . An inspection means for inspecting the added extract using an immunochromatography method or a nucleic acid chromatography, and an extraction container in which the extract is contained,
The inspection means includes a housing provided with an addition hole for adding the extract, a test strip accommodated in the housing and capable of developing the extract, and one end abutting the test strip. And an extract derivative having a capillary action embedded in the addition hole,
The extract derivative is a rod-shaped fiber bundle structure or sintered porous body having a pointed end with a sharpened tip,
The extraction container has a body part, a bottom part in which one end of the body part in the axial direction is sealed, and an opening provided at the other end in the axial direction of the body part,
The extraction container is configured to be able to communicate with the addition hole of the inspection unit in a sealing manner,
The inspection means includes a through hole forming means for forming a through hole in the bottom portion of the extraction container, and the extraction container and the inspection means are connected through the through hole formed by the through hole forming means. The addition hole is configured to communicate with the sealing hole in a sealing manner,
Due to the communication between the extraction container and the addition hole of the inspection means, the extraction liquid absorbed in the extraction liquid derivative moves in the extraction liquid derivative by capillary action and contacts the test strip. Configured to be added to the test strip through the one end of the liquid derivative;
The inspection device according to claim 1, wherein the through-hole forming means is a pointed portion of a rod-like fiber bundle structure or a sintered porous body which is the extract derivative. The bottom portion of the extraction container is provided with at least one bottom side engaging means disposed on an inner wall or an outer wall thereof,
Wherein the outer wall or inner wall of the added holes vicinity testing apparatus according to claim 1 or 2, characterized in that it comprises an additive bore-side engaging means for engaging the bottom side engagement means. The said extract derivative is supported and fixed by the extract derivative support means provided in the inside of the said addition hole of the said test | inspection means, The test | inspection of any one of Claims 1-3 characterized by the above-mentioned. apparatus. The extraction vessel, the inspection device according to any one of claims 1 to 4, characterized in that it is formed by a small non-flexible material with fluid-permeable.

Images