JP6071988B2 - Physical activity promoter - Google Patents

Description

  The present invention relates to a novel physical activity promoter and use thereof, and more particularly to a physical activity promoter containing milk enriched with lutein as an active ingredient and use thereof.

  Lutein is a kind of carotenoid that exists in the leaves, flowers, fruits and egg yolks of plants. Lutein has long been used as a food additive for coloring, but in recent years it has the effect of suppressing eye strain based on antioxidant action and cataract / aging age-related macular degeneration. Also attracting attention. For example, Patent Document 1 discloses a composition for eye strain containing lutein. Patent Document 2 discloses a nutritional supplement for treating macular degeneration containing lutein.

  On the other hand, in recent years, attention has been focused on attempts to increase the amount of physical activity as a measure against lifestyle-related diseases. According to Non-Patent Document 1, physical activity is a state of the body that involves the contraction of skeletal muscles and consumes more energy than at rest. Daily activities such as labor and housework in daily life and maintenance of physical fitness・ It is described that it can be divided into two types of exercises that are planned and intentionally implemented for the purpose of improvement.

  Various techniques are being developed for the purpose of preventing and treating lifestyle-related diseases by increasing the amount of physical activity. Patent Literature 3 and Patent Literature 4 disclose an apparatus and a system that improve an individual's motivation for activity by simply measuring the amount of physical activity. Patent Document 5 discloses a dietary supplement containing propionyl-L-carnitine or one of its salts, coenzyme Q10, nicotinamide, riboflavin, and pantothenic acid as a dietary supplement that promotes the ability of skeletal muscles to maintain physical activity. Is disclosed. This is a technique for increasing the amount of physical activity by ingesting a physiologically active substance. Patent Document 6 discloses the use of (−)-epigallocatechin gallate for endurance enhancement.

  Furthermore, it is known that astaxanthin, the same carotenoid as lutein, improves exercise endurance. Patent Document 7 discloses a pharmaceutical comprising astaxanthin as an active ingredient as a technique for improving the duration of muscle function in mammals and treating muscle disorders and the like. Non-patent document 2 describes that improvement in swimming endurance was observed in mice administered with astaxanthin.

  On the other hand, until now, there has been a report that lutein has no effect on physical activity as described above, but rather has no effect on oxidative injury and fatigue caused by exercise.

  By the way, milk (such as milk) is an important nutritional source for mammals before weaning, and is a food containing a good balance of protein, lipid, and sugar. In recent years, the effect of milk on physical activity has been reported. For example, Patent Document 8, Patent Document 9, Non-Patent Document 3, etc. have a function (action) to reduce muscle damage or promote muscle hypertrophy in casein and whey, which are proteins in milk. It has been reported.

  However, the influence of milk on lipid metabolism in the living body and the physiological activity produced when an antioxidant substance such as lutein and milk are ingested simultaneously have not been clarified.

JP 2007-308396 A JP-T-2004-520436 JP 2008-104758 A JP 2005-204918 A Special table 2009-512430 gazette JP 2008-545766 Gazette Special table 2001-514215 gazette JP 2002-65212 A Special table 2009-507044 gazette

Examination meeting for the development of exercise requirements and exercise guidelines, exercise standards for health promotion 2006-Physical Activity / Exercise / Physical Strength Report, issued in July 2006 Ikeuchi M et al. Biol. Pharm. Bull. , 29 (10), 2106-1110 (2006) Roy BD et al. , J .; Int. Soc. Sports Nutr. , 5:15 (2008)

  This invention is made | formed in view of the subject of said prior art, and it aims at providing the physical activity promoter which can be ingested highly active safely.

  In light of the above problems, the present inventors have conducted extensive research, and as a result, by simultaneously ingesting lutein and milk that did not sufficiently function with a single component, a highly active physical activity promoting effect is exhibited. As a result, the present invention has been completed. At this time, taking lutein and milk at the same time promotes the physical activity of the living body and increases the amount of physical activity, so the visceral fat amount reducing effect is exerted, and it is used as a preventive and therapeutic agent for lifestyle-related diseases. At the same time, we found out that it can be applied.

  In addition, when mothers who are pregnant or breastfeeding take lutein, not only their mothers but also their children can exert highly active physical activity promoting effects. The present inventors have found that it can be applied to agents and enhancers.

That is, this invention consists of the following.
[1] A physical activity promoter and / or a visceral fat mass reducing agent containing milk enriched with lutein as an active ingredient.
[2] The physical activity promoter and / or visceral fat mass reducing agent according to [1] above, wherein the milk enriched with lutein is milk to which lutein is added.
[3] The physical activity promoter and / or visceral fat mass reducing agent according to the above [1], wherein the milk enriched with lutein is milk secreted by a mammal who has taken lutein.
[4] The physical activity promoter and / or visceral fat mass reducing agent according to the above [1] to [3], wherein the milk enriched with lutein contains lutein at 200 μg / kg or more.
[5] A food / beverage product, a feed, and a medicine containing the physical activity promoter and / or visceral fat mass reducing agent according to [1] to [4].
[6] Food / beverage products, feed, and pharmaceuticals containing lutein for feeding to mammals secreting milk enriched with the lutein according to [3].
[7] A milk physical activity promoting action enhancer and / or visceral fat mass reducing action potentiator comprising lutein as an active ingredient.
[8] The physical activity promoting action enhancing agent and / or visceral fat mass reducing action enhancing agent of milk according to [7], comprising lutein at 200 μg / kg or more.

  According to the present invention, a physical activity promoter that is highly active and can be safely ingested can be provided. Moreover, according to this invention, the visceral fat amount reducing agent which promotes the physical activity of a biological body and increases a physical activity amount, and the preventive agent and therapeutic agent of a lifestyle-related disease can be provided.

  Furthermore, according to the present invention, since a highly active physical activity promoting effect is exerted not only on a pregnant or breastfeeding mother but also on the child, a physical activity promoter or enhancer for a child for a mother is provided. Can be provided.

It is a figure which shows the influence on the running amount of the rat which ingested the lutein enriched milk of Example 1. FIG. The upper diagram shows the amount of travel per hour, and the lower diagram shows the total amount of travel per day. It is a figure which shows the influence on the visceral fat amount of the rat which ingested the lutein enriched milk of Example 1. FIG. The vertical axis shows the amount of visceral fat per 100 g body weight. In the two bar graphs in each group, the left side represents the exercise group and the right side represents the non-exercise group. It is a figure which shows the influence on the gastrocnemius muscle weight of the rat which ingested the lutein enriched milk of Example 1. FIG. The vertical axis shows the gastrocnemius weight per 100 g of body weight. In the two bar graphs in each group, the left side represents the exercise group and the right side represents the non-exercise group. It is a figure which shows the anxiety behavior of the offspring mouse born from the mother mouse | mouth which ingested the lutein enrichment feed of Example 2. FIG. The vertical axis represents the number of times of entering the dark bar.

  The physical activity promoter and / or visceral fat mass reducing agent of the present invention contains milk enriched with lutein as an active ingredient. The physical activity promoter and / or visceral fat mass reducing agent of the present invention contains milk to which lutein is added as an active ingredient. On the other hand, the physical activity promoter and / or visceral fat amount reducing agent of the present invention contains milk secreted by a mammal ingesting lutein as an active ingredient.

  Here, the milk enriched with lutein (also referred to as lutein-enriched milk) means milk obtained by adding lutein to normal milk to increase the amount of lutein contained compared to normal milk. By the way, the content of lutein in normal milk is at most about 60 g / kg per milk fat (Calderon et al., J. Dairy. Sci., 90, 2335-2346 (2007)). It corresponds to about 120 μg / kg per milk. Moreover, the content of lutein in normal human milk (breast milk) is about 30 μg / kg at the most per milk (Japanese translations of PCT publication No. 2009-513641).

  Since the physical activity promoter and / or visceral fat content reducing agent of the present invention is more than the amount of lutein contained in normal milk and can be arbitrarily set as long as it exhibits the effect of promoting physical activity, lutein Is preferably 200 μg / kg or more (200 μg or more per kg), more preferably 2 mg / kg or more (2 mg or more per kg), more preferably 20 mg / kg or more (20 mg or more per kg), 50 mg / kg. The above (50 mg or more per kg) is particularly preferable. At this time, the upper limit is not particularly set as the amount containing lutein, but is preferably 5 g / kg or less, more preferably 1 g / kg or less, further preferably 500 mg / kg or less, and particularly preferably 200 mg / kg or less. .

  Here, the physical activity promoting agent and / or visceral fat amount reducing agent of the present invention is arbitrarily set as long as it is equivalent to the amount containing lutein as the amount to add (strengthen) lutein from the above viewpoint. Therefore, it is preferably 80 to 100 μg / kg or more, more preferably 2 mg / kg or more, further preferably 20 mg / kg or more, and particularly preferably 50 mg / kg or more. At this time, the amount of lutein to be added (strengthened) is, for example, preferably 5 g / kg or less, more preferably 1 g / kg or less, further preferably 500 mg / kg or less, and particularly preferably 200 mg / kg or less.

  The food / beverage products, feed and pharmaceuticals of the present invention contain the aforementioned physical activity promoter and / or visceral fat mass reducing agent. The food / beverage products, feeds, and pharmaceuticals of the present invention contain lutein for intake by mammals that secrete milk enriched with lutein.

  The physical activity promoting action enhancer and / or visceral fat mass reducing action enhancer of the present invention contains lutein as an active ingredient. Here, the physical activity promoting action enhancer of milk means that lutein enhances the physical activity promoting action inherent in milk by strengthening lutein into milk. Similarly, the visceral fat content reducing action enhancer of milk means that lutein enhances the visceral fat content reducing action inherent in milk by strengthening lutein into milk. That is, by combining milk and lutein, it is possible to enhance (promote) the functionality of lutein, which is a natural activity promoting action and / or a visceral fat content reducing action inherent in milk, and exhibit a remarkable effect.

  From the viewpoint equivalent to the physical activity promoter and / or visceral fat content reducing agent of the present invention, the physical activity promoting effect enhancer and / or visceral fat content reducing agent of milk of the present invention is an amount containing lutein. 200 μg / kg or more is preferred, 2 mg / kg or more is more preferred, 20 mg / kg or more is more preferred, and 50 mg / kg or more is particularly preferred. At this time, the amount containing lutein is preferably 5 g / kg or less, more preferably 1 g / kg or less, further preferably 500 mg / kg or less, and particularly preferably 200 mg / kg or less.

  Here, the physical activity promoting action enhancer and / or visceral fat content reducing action enhancer of the milk of the present invention is equivalent to the amount containing lutein as the amount to add (strengthen) lutein from the above viewpoint. 80 to 100 μg / kg or more is preferable, 2 mg / kg or more is more preferable, 20 mg / kg or more is further preferable, and 50 mg / kg or more is particularly preferable. At this time, the amount of lutein to be added (strengthened) is, for example, preferably 5 g / kg or less, more preferably 1 g / kg or less, further preferably 500 mg / kg or less, and particularly preferably 200 mg / kg or less.

  The lutein-enriched milk in the present invention may be raw milk or heat-treated milk (sterilized milk), and is produced by processing the milk with some milk products such as butter, cream, ice cream, cheese, yogurt, and lactic acid bacteria beverages. But it ’s okay. The lutein-enriched milk can be applied without limitation as long as it is mammalian milk, but is preferably human milk, cow milk, goat milk or the like that humans normally eat as food. In this lutein-enriched milk, plants such as corn and marigold and natural products such as egg yolk can be used as lutein as they are, or they may be used after being extracted or concentrated. Commercial products such as food additives can also be used as lutein, and the form of lutein may be any of free lutein, lutein ester, lutein salt and the like. Lutein-enriched milk can be prepared by adding these luteins to raw milk as they are, or by adding them to raw milk before processing, sterilized milk after processing, dairy products after processing, or the like.

  On the other hand, lutein-enriched milk can be prepared by ingesting lutein into a mammal and collecting the animal's milk. There is no limitation on the type of mammal, and it is preferably a person who secretes milk that humans normally eat as food, cows, goats and the like. From the above viewpoint, natural products containing lutein as lutein (for example, grass containing lutein in high concentration), extracts and concentrates derived from natural products, and commercial products can be used. Any of free lutein, lutein ester, lutein salt and the like may be used.

  As a method for ingesting lutein into mammals, from the viewpoint of reducing the burden on mammals, oral ingestion is preferable, and a method for ingesting food, feed, and pharmaceuticals containing lutein and lutein-enriched milk is particularly preferable. At this time, as long as the mammal secretes lutein-enriched milk, these foods, feeds, and pharmaceuticals can be used without limitation in kind.

  In the lutein enriched milk in this invention, it can measure by the well-known method about the quantity containing a lutein. Specifically, first, lutein-enriched milk is used as it is, or if necessary, purified by centrifugation, solvent extraction or the like to obtain a fraction containing lutein. Then, the content of lutein in the lutein-enriched milk can be measured by combining a fractionation method such as high performance liquid chromatography with a quantitative method such as absorbance of ultraviolet light or visible light, mass spectrometry. Thereby, the content of lutein necessary for the lutein-enriched milk to exhibit the effects of the present invention can be confirmed.

  In physical activity, the main energy sources of skeletal muscle are carbohydrates and fatty acids such as glucose and glycogen. It is known that when lipid metabolism is enhanced and lipid decomposition is promoted, the amount of fatty acid that can be used as an energy source increases, resulting in an increase in endurance and an increase in physical activity. When the physical activity promoter and / or visceral fat amount reducing agent of the present invention is ingested, the action of enhancing lipid metabolism and the support action of other physical activities occur in the living body, and as a result, the physical activity is promoted.

  In addition, when the physical activity promoter and / or visceral fat amount reducing agent of the present invention is ingested, it is quite surprising that in addition to being effective as a preventive or therapeutic agent for lifestyle-related diseases, for example, under stress conditions It has been confirmed that the physical activity promoting effect is exerted even in the state where physical activity such as is enhanced or suppressed.

  In recent years, incidents such as “cutting out suddenly” and “acting impulsively” have occurred frequently. As social backgrounds, lifestyles have diversified, working at night, lack of sleep, and sleeping even at night. There is a possibility that cases such as none are related. When the physical activity promoter and / or visceral fat mass reducing agent of the present invention is ingested, even in such cases, the effect of normalizing the physical activity is exhibited and the recovery from the stress condition (state) is achieved. I can urge you. In addition, when the physical activity promoter and / or visceral fat amount reducing agent of the present invention is ingested, not only the mother individual who ingested it, but also the child of the individual who ingested it, Effective through milk secreted by individuals. Therefore, the physical activity promoter and / or visceral fat amount reducing agent of the present invention can be used as a pharmaceutical, food, and feed for mothers for the purpose of promoting physical activity in children.

  In the lutein enriched milk in the present invention, the amount of physical activity can be measured by various known methods. For example, the physical activity index can be calculated from the physical activity time and its contents using the Met (META) which is the exercise intensity index described in Non-Patent Document 1, and the Mets / hour index obtained by multiplying the exercise time by Met. The amount can be measured easily. Moreover, the amount of physical activity can be measured using the apparatus described in Patent Document 3 or Patent Document 4. By measuring the amount of physical activity by these methods, the effect of the present invention can be evaluated and confirmed.

  The lutein-enriched milk in the present invention can be used as a composition by combining with a general excipient in addition to being used as a physical activity promoter and / or a visceral fat amount reducing agent as it is, and further, an external preparation for skin and an internal preparation , Injections, and other general dosage forms can be used. Excipients used in this formulation include, for example, oral solids such as tablets and capsules, internal liquids such as aqueous solutions and suspensions, ointments, patches, lotions, creams, sprays, suppositories, etc. Components that are widely known for other dosage forms and that are actually used may be appropriately combined.

  In addition to lutein-enriched milk as the above-mentioned composition and various dosage forms, if necessary, antioxidants such as vitamins, minerals, astaxanthin, and other pharmaceuticals can be added as active ingredients to make pharmaceuticals and quasi-drugs It may be in the form of a product. The amount of lutein-enriched milk blended as the above composition or various dosage forms is not particularly limited or defined, and varies slightly depending on the type and quality of the dosage form and the actual expected effect. The total amount is preferably 1% to 99% by weight, more preferably 10 to 99% by weight, still more preferably 50 to 99% by weight, and particularly preferably 80 to 99% by weight.

  The physical activity promoter and / or visceral fat amount reducing agent of the present invention may be used in the form of food or drink as it is, but may be used in a form combined with appropriate food or drink ingredients, specifically May be in the form of a food such as a beverage such as milk drink or juice, ice cream, cheese, yogurt, soup, jelly, jam, confectionery, bread, etc., or may be in the form of a health food or supplement. Moreover, since the effect of the present invention is promoted not only for adults but also for infants and women who have consumed breast milk, it may be in the form of infant food such as infant formula.

  The physical activity promoter and / or visceral fat amount reducing agent of the present invention may be used in the form of a feed as it is, but may be used in a form combined with an appropriate feed component, specifically, It may be in the form of feed for livestock such as cattle, goats, pigs and chickens, feed for aquaculture such as fish, and feed for pets such as dogs and cats.

  In the lutein-enriched milk in the present invention, since food is used as a raw material, when it is in the form of the above-mentioned food and drink, feed, etc., their intake is not particularly prescribed or restricted, but generally used as food for humans Desirably, it is preferably set within a range, and specifically, 1 to 1000 g is preferable, 10 to 500 g is more preferable, 50 to 250 g is more preferable, and more preferably 50 to 250 g per day in terms of lutein-enriched milk. The total intake is preferably 3 to 3000 g, more preferably 30 to 1500 g, and even more preferably 50 to 1000 g.

  Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not construed as being limited to such examples.

<Example 1>
Marigold dye containing 20% by weight of lutein (Lutein 20, Kyowa Wellness Co., Ltd.) is added to commercially available milk (Meiji delicious milk, Meiji Dairies Co., Ltd.) at 0.05% by weight, and lutein is 0.01%. Lutein-enriched milk (Lutein-enriched milk), containing by weight%, was produced.

<Example 2>
Lutein for ingestion of mother mice secreting milk enriched with lutein (lutein-enriched milk) by adding the marigold pigment of Example 1 to AIN-93G, which is a purified feed for laboratory animals, at 0.2% by weight. A fortified feed (Lutein fortified feed) was produced.

<Comparative Example 1>
The marigold pigment of Example 1 is added to a lecithin aqueous solution (lecithin concentration: 0.01% by weight) at 0.05% by weight, and contains lutein at 0.01% by weight. Fortified water).

<Test Example 1>
Forty three F344 rat male 3-week-olds were preliminarily reared in one week, and then divided into two groups that were reared in a spontaneous rotation running cage and a normal cage. Further, water (control), lutein-enriched water of Comparative Example 1 (Lutein concentration: 0.01 wt%), milk (Meiji delicious milk, Meiji Dairies Co., Ltd.), and Lutein-enriched milk of Example 1 (Lutein concentration: 0. (01% by weight) were divided into 4 groups, each of which was orally administered at 10 mL / kg-body weight, as test samples. The test breeding period was a dark period from 17:00 to 5:00 the next morning every day, food was withdrawn from 11:00 to 5:00 and fasted, and during the light period, movement to a rotating cage was prevented and the patient was rested. The test sample was orally administered through a feeding tube immediately before the dark period, and then a new food was given, and the amount of spontaneous rotation was measured after 30 minutes. The exercise amount, body weight and food intake were measured every day, and blood was collected from the abdominal aorta 9 weeks after the test, and the liver, gastrocnemius muscle and adipose tissue weight were measured. Neutral fat in the blood and liver, total cholesterol, and glycogen content in the coated muscle were measured. Furthermore, mRNA expression levels of AMPK, cpt1, PPARα, PPARδ, TNFα, FAS, IRS1, IGF1, and Glut4 in the liver and soleus muscle were measured using Real-time PCR.

  FIG. 1 shows a comparison result of the running amount in the eighth week of the test. In the two groups, the milk intake group and the lutein-enriched milk intake group, after the test sample was administered, the 2-hour running amount was significantly higher than that of the control group, and in the lutein-enriched milk group of Example 1, The total amount of exercise per day was also significantly higher. In the lutein-enriched water intake group of Comparative Example 1 in which lutein alone was administered, no increase in exercise amount was observed. In addition, when the expression level of each gene was investigated using Real-time PCR, the expression level of AMPK in the liver and soleus muscle showed a high value due to exercise and milk intake.

  The comparison result of the visceral fat amount per 100 g body weight at the time of dissection is shown in FIG. When compared between the groups without exercise, the milk intake group had a smaller average visceral fat mass than the control group, the lutein-enriched milk intake group of Example 1, and the lutein-enriched water intake group of Comparative Example 1 . When compared between groups loaded with exercise, the lutein-enriched milk intake group of Example 1 had a smaller average visceral fat amount than the control group, the lutein-enriched water intake group and the milk intake group of Comparative Example 1 . In addition, triglycerides and total cholesterol in blood and liver both showed significantly lower values by ingesting milk while exercising.

  The comparison result of the gastrocnemius muscle weight is shown in FIG. When compared between the groups loaded with exercise, the milk intake group had a higher average value of gastrocnemius muscle weight than the control group, the lutein-enriched milk intake group of Example 1, and the lutein-enriched water intake group of Comparative Example 1. In addition, glycogen content in gastrocnemius muscle showed a significantly high value by ingesting milk while applying exercise. In addition, there was no difference in final body weight among all groups.

  From the above results, it was revealed that lutein-enriched milk enhances lipid metabolism and increases physical activity. That is, it has been clarified that lutein enhances the lipid metabolism effect of milk (milk) and enhances the physical activity promoting action of milk (milk).

<Test Example 2>
For C57blck / 6J mice on the 14th day of pregnancy, with plug confirmation as day 0, 10 animals were divided into 5 groups of constant light (LL group) and light / dark group (DL group), and each group had 3 controls. The refined feed (AIN93) was fed (LL-cont group, LL-cont group), and the remaining 2 animals in each group were fed the lutein-enriched feed of Example 2 (LL-lutein group, DL-lutein group) ). They were allowed to eat freely with drinking water without restricting food consumption. In the DL group, the dark period was from 6:30 in the evening to 8:30 in the morning. In the constant light group, the animals were kept under constant light conditions from the 14th day of pregnancy to the 22nd day after the birth, with the date of birth being 0 days. After weaning, both groups had normal feeding conditions in which the light-dark cycle was from 6:30 in the evening to 6:30 in the morning.

  With regard to the 9-week-old female offspring bred in this way, an elevated plus maze test was carried out on each individual for 5 minutes (n = 6-8). Between each test, the runway was wiped with disinfecting ethanol to remove residual odor from the previous mouse. The elevated plus maze test verifies the degree of anxiety by observing the movement of animals between a dark bar covered with both walls and a light bar without walls, which are alternately installed in a cross shape. This method is generally used as a method for measuring anxiety behavior of small experimental animals. Since small experimental animals are not good at bright open spaces, it is evaluated that anxiety behavior increases when the stay time in the dark bar is long, and anxiety behavior decreases when the stay time is short.

  FIG. 4 shows a comparison result of the number of times of entering (darkening) the dark bar for the elevated plus maze test. In the control feed intake group without addition of lutein, the number of times entering the dark bar was significantly higher in the LL-cont group than in the DL-cont group (p = 0.0237). This test result shows that the anxiety behavior is increased after growth in mice subjected to constant light stress from the late fetal period to the feeding period, as compared to mice fed under normal light and dark conditions.

  In the DL group without constant light stress, the number of times of entering the dark bar was similar between the DL-cont group that received the control diet and the DL-lutein group that received the lutein-enriched diet. On the other hand, in the LL group with constant light stress, the number of times of entering the dark bar was significantly less in the LL-lutein group ingested lutein-enriched diet than in the LL-cont group ingested control diet (p = 0.0019).

  In addition, the number and time of appearing in the light bar were the smallest (short) in the LL-cont group, although there were no significant differences between the groups, but there were 2 mice that never appeared in the light bar. In contrast, in the LL-lutein group, as much (longer) as in the DL-cont and DL-lutein groups, it was observed that all mice appeared more than twice in the light bar. It was done.

  From the above results, it became clear that anxiety behavior due to constant light stress was suppressed and physical activity was normalized in pups born from mother mice fed lutein-enriched (lutein-added) feed. That is, it was revealed that a child born from a mother who sufficiently consumed lutein increased mental calmness (mental stability) and enhanced physical activity promoting action.

Claims (5)

Comprising added pressure of lutein in milk for mothers ingested in pregnant or nursing, results in anxiety inhibiting effect on children being born nursing from the mothers by mother ingested to pregnant or nursing A method for imparting or enhancing an action to the milk. Adding lutein in 80 [mu] g / kg or more milk The method of claim 1. Use of lutein to produce lutein-enriched milk for producing an anxiety-inhibiting effect on a child born and breastfeeded by the mother during pregnancy and breastfeeding . The use according to claim 3 , wherein the lutein-enriched milk is obtained by adding lutein to the milk at 80 µg / kg or more. Lutein-enriched milk containing lutein of 2 mg / kg or more and 500 mg / kg or less for bringing anxiety behavior suppressing effect to a child born and breast-feeded by the mother during ingestion and breastfeeding .