JP5847518B2 - Method for producing NK cell-enhanced blood product - Google Patents

Description

  The present invention relates to a method for producing a blood product in which NK cells are activated and proliferated, and the blood product and a composition for NK cell activity.

  Cancer, a malignant neoplasm, has been the leading cause of death among Japanese since 1981, accounting for about 30% of all causes of death. Although medical cures and survival rates have improved significantly due to advances in medicine, it remains a refractory disease. As a cancer treatment method, surgical treatment, chemotherapy, and radiation therapy are standard treatment methods, but in recent years, immunotherapy has attracted attention as a new treatment method (Non-patent Document 1).

  Immunotherapy is a method for treating cancer, viral infections, and the like by immunity in the body. In particular, for cancer, it has attracted attention as a new treatment method along with surgical therapy, chemotherapy, and radiation therapy, and various methods have been developed so far. For example, cytokine therapy, vaccine therapy, BRM (Biological Response Modifier) therapy, cellular immunotherapy and the like can be mentioned.

  Cytokine therapy is a treatment method that kills cancer cells and virus-infected cells by directly administering in vivo a cytokine having an action of proliferating or activating lymphocytes such as T cells and NK cells. For example, cytokine therapy by administration of interleukin 2 (IL-2) or interferon is applicable (non-cited document 2). However, this therapy is not as effective as expected in clinical results, and severe such as organ dysfunction, fluid retention (in the case of IL-2 administration), cold symptoms or mental disorders (in the case of interferon administration) There was a problem of causing side effects.

  Vaccine therapy is a treatment that directly or indirectly inoculates a cancer cell-specific antigen or peptide and activates the immune system against the antigen (Non-patent Document 3). Although several effective cases have been reported for this treatment method, there are problems such as ineffectiveness for tumors that do not express HLA class I.

  BRM therapy is a treatment method using a substance that modifies a patient's biological responsiveness to tumor cells and the like (Non-patent Document 4). Known BRMs include PSK, bestatin, and OK432. Although this therapy is effective in some cancers, it can be used effectively in combination with other therapies that reduce immunity, such as surgery and chemotherapy. Strong aspect of physical therapy. In addition, immunity is not necessarily strengthened, and there is a problem that the anticancer effect alone is weak.

  Cellular immunotherapy is a treatment method that enhances the immunity of a patient by activating and proliferating the immune cells collected from the patient in vitro and then returning it to the patient's body. It is also called "therapy (adoptive immunotherapy in a broad sense)" (Non-patent Document 5). Cellular immunotherapy is classified into activated lymphocyte therapy and dendritic cell therapy depending on the type of immune cells treated in vitro. Of these, dendritic cell therapy has just started clinical trials, and has not yet obtained sufficient results to determine its effectiveness.

  Activated lymphocyte therapy is active against NK cells and activated lymphocyte therapy in a narrow sense (activated T lymphocyte therapy or narrowly adoptive immunotherapy) in which T cells are activated and proliferated in vitro. -It is further classified into activated NK cell therapy with proliferation treatment.

  The narrowly-defined activated lymphocyte therapy includes, for example, LAK (lymphokine activated killer cell) therapy, TIL (tumor tissue infiltrating lymphocyte) therapy, CTL (cytotoxic lymphocyte) therapy, and the like.

  LAK therapy is a method of adding a large amount of IL-2 to lymphocytes collected from a patient, culturing them, activating or proliferating T cells and NK cells, and then returning them to the body (Non-patent Document 6). This method requires administration of a large amount of IL-2 in vivo in order to maintain the LAK activity administered in vivo. There was a problem that the effect of could not be obtained.

  TIL therapy is a method of collecting lymphocytes infiltrating tumor tissues and the like, cultivating them outside the body in the same manner as LAK therapy, and then returning them to the body (Non-patent Document 7). However, this method has a problem that lymphocytes can be collected only from surgically-extracted tissues and an effect as expected cannot be obtained.

  CTL therapy is a method of inducing lymphocytes specific to cancer cells and the like by culturing cancer cells collected by surgery with lymphocytes and stimulating lymphocytes (Non-patent Document 8). Although there are reports of effective cases, cancer cells must be collected by surgery, which is highly invasive and limited to indications. In addition, if cancer cells cannot be collected and cultured, treatment is difficult, There are problems such as being effective only for cancers that express histocompatibility antigens.

  On the other hand, activated NK cell therapy is a method of transferring proliferated and activated NK cells into the body. NK cells are a lymphocyte population that can kill cancer cells and virus-infected cells without being sensitized to antigens (Non-Patent Documents 9 to 11). In animal experiments, it is known that cancer invasion and metastasis can be suppressed (Non-patent Document 12), and in long-term large-scale cohort studies, people with higher NK cell activity in peripheral blood develop cancer than those with lower activity. There is also a report that the rate is significantly low (Non-patent Document 13). Therefore, if a patient's NK cells are proliferated and activated in large quantities outside the body and then transferred into the patient's body, cancer, viral infections, etc. can be treated. Activated NK cell therapy is a treatment method based on such a principle. Generally, NK cells are present only in a few to a few dozen percent in lymphocytes of healthy individuals, and the number of NK cells is further reduced in the case of cancer patients. In addition, the cancer cytotoxicity of NK cells in blood is often lower in cancer patients than in healthy individuals even if the number of NK cells is the same. Therefore, growth and activation by culture are essential. Conventionally, NK cell growth in vitro has been difficult, but many recent studies have reported successful examples of NK cell growth culture (Non-Patent Documents 14 to 17). However, these methods use cancer cells and gene-transferred cells cultured to strengthen NK cells, and have unsolved problems in safety and practicality at the time of clinical application. In addition, the NK cell proliferation efficiency and cell activity were not sufficiently satisfied.

  As described above, the conventional immunotherapy has a problem that none of the methods can provide a sufficient therapeutic effect, has serious side effects, or has other problems to be improved.

  Therefore, as a result of intensive studies to solve the above problems, the inventors of the present application processed NK cells in blood collected from a living body together with a stimulating factor that proliferates NK cells at a specific temperature for a specific time. We have succeeded in developing a method for producing an NK cell-enhanced blood product that can be efficiently enhanced, and obtained a patent for the production method and NK cell-enhanced blood product (Patent Document 1). The NK cell-enhanced blood product obtained by this method is actually used in the clinical stage, and many very good clinical results have been obtained (Non-patent Documents 18 to 20). However, in this production method, the production process is somewhat complicated, and in order to sufficiently activate NK cells, the medium must be maintained at a specific temperature for a relatively long time (10 to 30 hours). There was a problem that it took time and effort to complete the preparation and the preparation.

Patent No. 4275680

Milani V, et al., 2009, J trans Res, 7 (50): 1-18. Rosenberg SA, et al., 1985, J Exp Med., 161: 1169-88. Bendandi, M. et al., 1999, Nature Med, 5: 1171-1177. Fisher M, et al., 2002, Anticancer Res., 22: 1737-54. Takayama Y et al., 2000, Lancet, 356: 802-807. Mule JJ, et al., 1985, J Immunol., 135: 646-52. Dudley ME, et al., 2003, J Immunother., 26: 332-42. Araki K et al., 2000, Int J Oncol., 17 (6): 1107-18. Stagg J and Smyth M J., 2007, Drug News Perspect, 20 (3): 155-163. Terme M et al., 2008 Nat. Immunol, 9 (5): 486-493. Vivier E et al., 2008, Nat.Immunol, 9 (5): 503-510. Dewan MZ et al., 2007, Breast Cancer Res Treat, 104: 267-275. Imai K et al., 2000, Lancet, 356: 1795-1799. Harada H et al., 2002, JPN J Cancer Res, 93: 313-9. Carlens S et al., 2001 Hum Immunol, 62: 1092-8. Berg M et al., 2009, Cytotherapy, 11 (3): 341-55. Fujisaki H et al., 2009, Cancer Res, 9: 4010-7. Brillard E et al., 2007, Exp Hemato, 35: 416-425. Cooke A and Brode S., 2008, Critical Rev immununol., 28 (2): 109-126. Hsu KC et al., 2005, Blood, 105: 4878-4884.

  The present invention provides a method for producing a new NK cell-enhanced blood product that has low invasiveness to donors and patients, has a simple production process, and can rapidly and intensively enhance NK cells in blood collected from a living body. It is an object to develop and provide a NK cell-enhanced blood product obtained by the method in a safe and relatively inexpensive manner.

  In order to solve the above problems, the present inventors have conducted further research on a method for producing an NK cell-enhanced blood product, and as a result, the previous application cited in Patent Document 1 (hereinafter referred to as “the previous application”). A new NK cell-enhanced type that enhances NK cell proliferative activity without requiring a step of holding for a specific time at a specific temperature, which was an essential step in the manufacturing method of NK cell-enhanced blood products Succeeded in developing a method for manufacturing blood products.

The present invention has been completed based on the development results and provides the following.
(1) A stimulation process in which NK cells contained in blood collected from a living body are stimulated with an NK cell proliferation stimulating factor including anti-CD16 antibody, OK432, anti-CD137 antibody, and cytokine, and the blood is physiological after the stimulation process A method for producing an NK cell-enhanced blood product comprising a culture step of culturing at cell temperature.
(2) The production method according to (1), wherein the cytokine is IL-2.
(3) The production method according to (1) or (2), wherein the physiological cell temperature is 36.5 to 37.5 ° C.
(4) The production method according to any one of (1) to (3), wherein a culture period in the culture step is 7 to 30 days.
(5) The production method according to any one of (1) to (4), wherein the anti-CD16 antibody is immobilized on a support.
(6) A NK cell-enhanced blood product obtained by the production method according to any one of (1) to (5).
(7) A composition for enhancing NK cells, comprising anti-CD16 antibody, OK432, anti-CD137 antibody and cytokine.
(8) The composition according to (7), wherein the cytokine is IL-2.
(9) A kit for producing NK cell-enhanced blood comprising the composition for enhancing NK cell according to any one of (7) to (8).

  According to the method for producing a NK cell-enhanced blood product of the present invention, NK cells in blood can be prepared more quickly and easily than conventional methods, and the proliferation rate of NK cells can be further improved. Moreover, according to this manufacturing method, since it can manufacture from peripheral blood, there exists an advantage that the invasiveness with respect to a donor and a patient is low.

The cytogram of the culture | cultivation 0th day, the 14th day, and the 21st day in the sample a (upper stage) and the sample b (lower stage) of an Example is shown. In each cytogram, the horizontal axis represents PC5 label-anti-CD3 antibody (day 0), ECD label-anti-CD3 antibody (day 14 and 21), and the vertical axis represents PE label-anti-CD56 antibody (day 0). ), The fluorescence intensity of the PC5-labeled anti-CD56 antibody (14th and 21st days) is shown on a logarithmic scale. The cytogram was fractionated into four compartments (B1 to B4) based on the intensity of each fluorescence. B1 (CD3 ^- CD56 ⁺⁾ The NK cells, B2 (CD3 ⁺ CD56 ⁺⁾ and B4 (CD3 ⁺ CD56 ^-) The T lymphocytes, and B3 (CD3 ^- CD56 ^-) in the above, such as B cell Non-cells are distributed. The numerical value in each fraction represents the ratio (%) of cells contained in the fraction in the measured cultured cells. It is a cell growth curve which shows the relationship between the culture | cultivation days and the culture total cell number in sample a and b of an Example. The cytotoxic activity of NK cells on day 14 and day 21 of culture in samples a and b of Examples is shown. The E / T ratio on the X axis is the ratio of cultured NK cells used as effector cells (E) to target K562 cells (target cells: T). The Y axis shows the cytotoxic activity of NK cells against K562 as a relative value (%) relative to the control before injury without adding effector cells.

1. 1. Production method of NK cell-enhanced blood product 1-1. Outline The first embodiment of the present invention is a method for producing an NK cell-enhanced blood product. In the present embodiment, NK cells in blood collected from a living body are stimulated with a growth stimulating factor, and then cultured at a physiological cell temperature.

  In the present invention, “enhancement” means that a cell is proliferated and / or activated. As used herein, “activation” of a cell means that the function of a cell, particularly a NK cell, is enhanced or increased. Examples thereof include enhancement / increase of expression of receptors relating to cytotoxic function, NK cell surface activity and / or proliferation. In the present invention, the “NK cell-enhanced blood preparation” refers to a preparation mainly composed of blood containing a number of activated NK cells obtained by the production method of the present embodiment.

1-2. Configuration The production method of the NK cell-enhanced blood product of this embodiment includes a stimulation step and a culture step. Hereinafter, the configuration of each process will be specifically described. In the present embodiment, it is assumed that each operation uses a sterilized reagent, medium, instrument, etc. in principle, and the culture is performed in an aseptic environment such as a clean bench in a clean room. This is to prevent contamination such as bacteria.

1-2-1. Stimulation process The “stimulation process” is a process of stimulating NK cells contained in blood collected from a living body with a NK cell proliferation stimulating factor.
(1) Blood In the present invention, “blood” refers to a blood component containing NK cells. For example, whole blood, umbilical cord blood, bone marrow fluid, and some of its components, such as mononuclear cells, are applicable. Although any blood can be used, mononuclear cells are preferred because blood components such as red blood cells and granulocytes can be an inhibitory factor in the production of NK cell-enhanced blood products. Among these, peripheral blood mononuclear cells (hereinafter referred to as “PBMCs”) obtained from peripheral blood are particularly preferable. This is because peripheral blood can be easily collected from a living body at any time and has low invasiveness to a donor.

  In the present invention, “living body” refers to a living mammal. Mammals are not limited, but are preferably humans. The target living body is desirably the same type as the mammal to which the NK cell-enhanced blood product obtained by the production method of the present invention is administered. For example, when the NK cell-enhanced blood product of the present invention is administered to a human, the blood is preferably collected from the human. More preferably, it is collected from a donor compatible with the recipient's HLA (human leukocyte antigen) genotype. For example, if the recipient has performed organ transplantation or stem cell transplantation, the donor is usually applicable, and in many cases, the related person is applicable. This is because a blood product derived from the blood of a donor that matches the HLA genotype can completely eliminate the possibility of rejection after administration. Therefore, it is most preferable that the recipient himself / herself who administers the NK cell-enhanced blood product of the present invention becomes the donor, that is, blood collection is performed on the premise of adoptive immunotherapy. When adoptive adoptive immunotherapy is assumed, the living body to be collected does not have to be a healthy body. For example, blood collected from a donor suffering from cancer or viral infection may be used. In the present invention, adoptive immunotherapy means the above-described adoptive immunotherapy in the broad sense unless otherwise specified.

  “Collected from a living body” means being derived from a living body. For example, in addition to those collected by inserting a needle into a living body directly, such as peripheral blood or bone marrow fluid, or those collected directly from the umbilical cord after delivery, such as umbilical cord blood, or from the reflux of transplanted organs It is done. Blood collected from preserved blood or the like that has been stored once refrigerated or frozen after heparin is added to the collected blood and subjected to anticoagulation treatment or after further isolation of mononuclear cells Also good.

(2) Preparation of blood When the blood used in this step is directly collected from a living body, the collection method may follow a known blood collection method. For example, peripheral blood may be collected by injection into a peripheral vein, etc., bone marrow fluid may be collected by bone marrow puncture (mark), and umbilical cord blood may be collected before delivery of the postpartum placenta. The needle can be collected by inserting a needle into the umbilical cord. Hereinafter, the collection of peripheral blood will be specifically described with an example.

  Peripheral whole blood can be collected by a known whole blood collecting method using a vacuum blood collection tube, a blood collection bag or the like by inserting an injection needle into a peripheral blood vessel of a living body, for example, a vein or an artery. The volume to be collected varies depending on the amount of NK cell-enhanced blood product required, but for example, when producing the blood product to be administered once to one adult, it may usually be 20 mL to 60 mL. However, when the number of PBMCs in the blood is extremely reduced as in cancer patients, only the necessary amount of PBMCs may be selectively collected by blood sampling (apheresis). After collection, for example, the inside of the blood collection syringe is coated with a blood coagulation inhibitor or blood coagulation inhibitor such as heparin in advance, or heparin or the like is added to the collected blood. It is preferable. Alternatively, plasma may be separated from peripheral whole blood and only blood cell components may be used. Separation of plasma can be achieved, for example, by transferring peripheral whole blood to a centrifuge tube, centrifuging at 2000 rpm to 4000 rpm for 5 to 20 minutes, and removing the supernatant. The separated plasma is heated at 56 ° C for about 30 minutes to be inactivated, and centrifuged at 2000 rpm to 4000 rpm for 5 to 20 minutes to remove precipitates such as platelets, so that it can be used as a nutrient source for cell culture. You can also.

  If necessary, further PBMCs may be separated from peripheral whole blood. PBMCs can be obtained from peripheral whole blood or blood cell components after plasma separation using density gradient centrifugation using Ficoll-Hypaque or Ficoll-Conray as a specific gravity solution. For these specific gravity liquids, it is convenient to use a commercially available separation liquid or the like. For example, Ficoll-Paque PLUS (Amersham) or LYMPHOPREP (AXIS-SHIELD) can be used. For the method of separating PBMCs, the protocol attached to the kit may be followed.

The PBMCs after separation are washed several times with PBS (-) or a culture medium for cultured cells to remove the specific gravity solution. Here, as the culture medium for cultured cells, for example, PBS (−) without serum, RPMI1640 medium, serum-free medium used for culture, or the like may be used. It is preferable to count the number of recovered PBMCs after washing with PBS (−) or a medium using a hemocytometer. For healthy human adults, usually 2 × 10 ⁷ or more PBMCs can be recovered from 20 to 60 mL of peripheral whole blood.

When the blood used in this step is frozen or refrigerated blood, it may be used after being thawed and heated by a known method. For example, there is a method in which RPMI-1640 medium is added to frozen PBMCs and thawed, and then incubated at 37 ° C. under 5% CO ₂ for 3 hours.

(3) NK cell proliferation stimulating factor In the present invention, “NK cell proliferation stimulating factor” refers to a factor that directly or indirectly enhances NK cells. Factors that directly enhance include, for example, factors that have a function of transmitting proliferation signals or activation signals into the cells of NK cells by specifically binding to receptors on the surface of NK cells. . Indirectly induced factors include, for example, factors that induce production and release of humoral factors such as cytokines by binding to cell surface receptors such as monocytes other than NK cells. NK cells will be indirectly enhanced by released humoral factors.

  The NK cell proliferation stimulating factor of the present invention contains anti-CD16 antibody, anti-CD137 antibody, OK432 and cytokine as essential factors.

  An “anti-CD16 antibody” is an antibody against CD16 antigen. The CD16 antigen is a marker for NK cells and granulocytes, and is known as a constituent protein FcγRIII of Fc receptors present on the cell surface of most NK cells in the resting phase. The NK cell proliferation-inducing activity of the anti-CD16 antibody was found in the previous patent application and was not previously known. The mechanism of NK cell proliferation induction by anti-CD16 antibody has not been clarified, but by adding anti-CD16 antibody together with cytokines such as IL-2, the proliferation of NK cells compared to the case where cytokine alone was added. The induction rate can be dramatically increased (Japanese Patent Application 2006-124630; Non-Patent Document 1). This antibody may be any of a monoclonal antibody, a polyclonal antibody, and fragments thereof.

In the present specification, the “fragment thereof” refers to a partial fragment of a polyclonal antibody or a monoclonal antibody, and refers to a polypeptide chain or a complex thereof having an activity substantially equivalent to the antigen-specific binding activity of the antibody. Say. For example, an antibody portion including at least one antigen-binding site described above, that is, a polypeptide chain having at least one pair of light chain variable region (VL) and heavy chain variable region (VH) or a complex thereof is applicable. Specific examples include a number of well-characterized antibody fragments generated by cleaving immunoglobulins with various peptidases. For example, Fab, F (ab ′) ₂ , Fab ′ and the like are applicable. Each of these antibody fragments includes an antigen-binding site and has the ability to specifically bind to an antigen (ie, CD16 here).

  In the present specification, the monoclonal antibody may be a synthetic antibody synthesized chemically or by using a recombinant DNA method. For example, the antibody constructed | assembled using the recombinant DNA method is mentioned. Specifically, although not limited, a monomeric polypeptide in which one or more VL and one or more VH of the monoclonal antibody of the present invention are artificially linked via a linker peptide having an appropriate length and sequence, etc. It corresponds to a molecule or a multimeric polypeptide (multivalent antibody). Examples of such polypeptides include single chain Fv (scFv: single chain fragment of variable region), diabody, triabody, tetrabody and the like. Bivalent or more multivalent antibodies such as diabody do not require each antigen binding site to bind to the same epitope, and each has a multispecificity that recognizes and specifically binds to a different epitope. It doesn't matter. As the anti-CD16 antibody of the present invention, a preferred antibody is a monoclonal antibody, that is, an anti-CD16 monoclonal antibody. An anti-human CD16 monoclonal antibody using human CD16 as an antigen is particularly preferred. Commercially available products can be used for such antibodies. For example, in the case of an anti-human CD16 monoclonal antibody, 3G8, B73.1 and the like can be mentioned.

  An “anti-CD137 antibody” is an antibody against CD137 antigen. CD137 antigen is a 30 kDa glycoprotein belonging to the TNF receptor superfamily of costimulatory molecular groups. Activation by anti-CD137 antibody has been shown to contribute to T cell activation and maintenance of active and memory T cells (Schwarz H, et al. 1996, Blood 87: 2839-2845; Croft M , et al. 2003; Nat Rev Immulo. 3: 609-620). On the other hand, there has been no report that has proved activation of human NK cells (Baessler T, et al. 2010; Blood 115: 3058-3069). The anti-CD137 antibody used for the NK cell proliferation stimulating factor of the present invention is not particularly limited as long as it is an antibody that specifically recognizes and binds to the CD137 antigen. It may include monoclonal antibodies, polyclonal antibodies, and fragments thereof. A monoclonal antibody is preferable. A commercially available antibody can also be used as the monoclonal antibody of the present invention. For example, if it is an anti-human CD137 monoclonal antibody, 4-1BB, G6, 4B4-1, ON185, BBK-2, C-20, D-20, G-1, N-16, BBEX2 or Lq-14 etc. Is mentioned.

  “OK432” (trade name: Picibanil) is an antitumor agent comprising a Streptococcus pyogenes A group 3 type Su strain treated with penicillin as an active ingredient, and belongs to the aforementioned BRM. “BRM” refers to a substance that, as described above, provides a therapeutic effect by modifying the host's biological response to tumor cells. OK432 is known as an immune adjuvant that binds to cell surface TLRs such as monocytes to activate monocyte cells and activate immune responses (Ryoma Y, et al., 2004, Anticancer Res., 24: 3295-301.).

  “Cytokine” is a wide variety of proteinaceous hormones responsible for information transmission between cells, and has the effect of strengthening lymphocytes such as T cells and NK cells in the immune system as described above. For example, interleukin (Interleukin), interferon (INF), TNF, MCP, etc. are mentioned. Cytokines suitable as the NK cell proliferation stimulating factor of the present invention include, for example, interleukin 2 (hereinafter referred to as “IL-2”, and the same applies to other interleukins), IL-12, and IL-15. IL-18, TNF-α, IL-1β and the like. Of these, IL-2 is a particularly preferred cytokine in the present invention.

  The NK cell proliferation stimulating factor of the present invention, in addition to the above essential factors, may further be an anti-CD3 antibody, a bisphosphonate derivative or a salt thereof or a hydrate thereof (hereinafter referred to as “bisphosphonate derivative etc.”). ) And / or BRM other than OK432.

  An “anti-CD3 antibody” is an antibody against CD3. The anti-CD3 antibody used for the NK cell proliferation stimulating factor of the present invention is not particularly limited as long as it is an antibody that specifically recognizes and binds to CD3. Either a monoclonal antibody or a polyclonal antibody may be used. A monoclonal antibody is preferable. For example, muromonab CD3 (trade name: Orthoclone OKT3 (registered trademark), Janssen Pharma) is mentioned.

The “bisphosphonate derivative” refers to a compound represented by the following general formula 1.

In the above formula, R ₁ represents a hydrogen atom (H) or a lower alkyl group, and R ₂ and R ₃ are each independently a hydrogen atom, halogen, hydroxyl group, amino group, thiol group, substituted or Represents an unsubstituted aryl group, a substituted or unsubstituted alkyl group, a lower alkylamino group, an aralkyl group, a cycloalkyl group or a heterocyclic group, or R ₂ and R ₃ are cyclic containing them Substituents that form part of the structure and form the cyclic structure are each independently a halogen, lower alkyl group, hydroxyl group, thiol group, amino group, alkoxy group, aryl group in R ₂ and R ₃ , Arylthio group, aryloxy group, alkylthio group, cycloalkyl group or heterocyclic group.

  Specific examples of the bisphosphonate derivative include zoledronic acid, pamidronic acid, alendronic acid, risedronic acid, ipandronic acid, incadronic acid, and etidronic acid.

  In the present invention, one or more bisphosphonate derivatives and the like can be added as a NK cell proliferation stimulating factor. In the present invention, a particularly preferred bisphosphonate derivative is zoledronic acid or a zoledronic acid derivative having a potentiating activity on NK cells, or a salt or hydrate thereof.

  Zoledronic acid (trade name: Zometa (registered trademark), Novartis Pharma Co., Ltd.) is a bisphosphonate having bone resorption inhibitory activity. Bone lesions caused by hypercalcemia due to malignant tumors or multiple myeloma and bone lesions caused by solid cancer bone metastasis It is known as a therapeutic drug. In addition, since its chemical structure includes nitrogen-containing bisphosphonates (N-BPs), it suppresses the synthesis of Farnesyl PyrophosPhate (FPP) in the cell, and as a result, the precursor IsoPentenyl Pyrophosphate (IPP) accumulates. It can activate the immune response of the living body (van Beek E, et al., 1999, Biochem Biophys Res Commun, 264: 108-11; Gober HJ, et al., 2003, J Exp Med, 197: 163 -8.) And enhancing the proliferation activity of γδ T cells (Sato K, et al., 2005, Int. J. Cancer, 116: 94-99; Kondo M, et al., 2008, Cytotherapy, 10 (8): 842-856.) Has been reported.

  “Salt thereof” refers to a basic addition salt of the bisphosphonate derivative, preferably zoledronic acid. Examples of basic addition salts include alkali metal salts such as sodium salt or potassium salt, alkaline earth metal salts such as calcium salt or magnesium salt, trimethylamine salt, triethylamine salt, dicyclohexylamine salt, ethanolamine salt, diethanolamine. Salt, aliphatic amine salt such as triethanolamine salt or brocaine salt, aralkylamine salt such as N, N-dibenzylethylenediamine, heterocyclic aromatic amine such as pyridine salt, picoline salt, quinoline salt or isoquinoline salt Salt, basic amino acid salt such as arginine salt or lysine salt, ammonium salt or tetramethylammonium salt, tetraethylammonium salt, benzyltrimethylammonium salt, benzyltriethylammonium salt, benzi Tributylammonium salts include quaternary ammonium salts such as methyl trioctyl ammonium salts or tetrabutylammonium salts.

  “BRMs other than OK432” include, for example, protein polysaccharide complexes extracted from filamentous fungi, more specifically lentinan extracted from shiitake mushrooms and krestin extracted from kawaratake.

(4) Stimulation method “Stimulate” refers to strengthening NK cells by bringing the NK cell proliferation stimulating factor into contact with NK cells.

As a specific stimulation method, first, blood collected from a living body, for example, PBMCs is prepared in a medium so as to have a cell density of 1 to 3 × 10 ⁶ cells / mL, for example. As the medium used here, a medium obtained by adding about 5 to 10% by volume ratio (V / V) of inactivated human serum or plasma to an appropriate medium for cell culture may be used. When administration by the adoptive immunotherapy is premised, it is desirable to use the medium obtained by adding autologous plasma to a serum-free medium for adoptive immunotherapy such as OpTmizer. Autologous plasma may be prepared from blood obtained after the blood collection step, as described above. For example, the supernatant obtained by centrifuging the collected peripheral whole blood at room temperature (10 ° C. to 30 ° C .: the same applies below) at 3000 rpm for about 10 minutes can be used as autologous plasma. Moreover, you may add antibiotics, such as streptomycin, penicillin, kanamycin, and gentamicin, to a culture medium as needed.

Next, a NK cell growth stimulating factor is added to the culture solution containing PBMCs prepared in advance.
Stimulation with an anti-CD16 antibody is added directly to the medium so that the final concentration is, for example, 0.01 μg / mL to 100 μg / mL, preferably 0.1 μg / mL to 10 μg / mL, more preferably 1 μg / mL, Alternatively, the antibody may be added in a state of being immobilized on a support. Preferably, the addition is performed in a state where the support is solid-phased. This is because by immobilizing the anti-CD16 antibody, the frequency of contact with NK cells from a certain direction is increased, and proliferation stimulation can be applied to NK cells more efficiently than in the free state. The “support” here is a scaffold for immobilizing an antibody. The material of the support is not particularly limited as long as it can fix the antibody in a stable state. For example, synthetic resin such as plastic, glass, metal and the like can be used. The shape of the support is not particularly limited, but is preferably a shape having a large contact surface area with the culture solution so that the contact frequency between the antibody immobilized on the support and NK cells is increased. Examples thereof include a spherical bead and a porous cube having a lymphocyte-sized pore.

The method of immobilizing the anti-CD16 antibody on the support is simply contacting the contact with the antibody solution (soaking, coating, distribution, etc.) if the support is made of a material having high affinity to the antibody, such as plastic. It can be fixed simply by holding it at a predetermined temperature and time. The anti-CD16 antibody solution is prepared by, for example, sterilizing the anti-CD16 antibody with sterile distilled water or cell culture medium, and then sterilizing by filtration with, for example, a 0.22 μm pore size filter to a final concentration of 1 μg / mL. Thus, it can be obtained by adjusting with sterile distilled water or medium. When the anti-CD16 antibody is immobilized on a support, it is preferable to use an anti-CD16 antibody solution having a liquid volume in consideration of the surface area of the support to be immobilized. For example, when solid-phased in a plastic flask having an inner wall surface area of 150 cm ² , about 15 mL of 1 μg / mL anti-CD16 antibody solution may be used. In the case of 25 cm ² and 75 cm ² culture flasks, 5 mL and 10 mL may be used, respectively. Thereafter, when incubated at 37 ° C. for 12 to 24 hours, the anti-CD16 antibody adheres to the support. Alternatively, a commercially available antibody immobilization kit or the like may be used. For example, CarboLink (PIERCE) can be used. Such an immobilization kit is useful when the support is made of a material that is difficult for antibodies to adhere to.

  After immobilizing the anti-CD16 antibody on the support, it is desirable to wash the support on which the anti-CD16 antibody is immobilized as necessary to remove the anti-CD16 antibody solution. For example, the support may be washed several times with an appropriate amount of PBS, for example, about 2 to 5 times. The culture vessel in which the anti-CD16 antibody is immobilized on the support in this manner reduces or deactivates the antibody activity for about 1 month by storing at 0 ° C to 8 ° C, preferably 3 ° C to 6 ° C. Can be used without

  Stimulation with an anti-CD137 antibody may be added to a culture solution containing PBMCs such that the final concentration is 0.1 μg / mL to 10 μg / mL. This is because if it is less than 0.1 μg / mL, it is insufficient for inducing growth stimulation, and if it is more than 10 μg / mL, the growth of NK cells is conversely suppressed. Preferably, it is 0.3 μg / mL to 6 μg / mL, more preferably 1 μg / mL to 3 μg / mL.

  Stimulation with OK432 is carried out in a culture solution containing PBMCs, for example, by adding OK432 solution at a final concentration of 0.005 KE / mL to 0.05 KE / mL, preferably 0.008 KE / mL to 0.015 KE / mL, more preferably 0.01 KE / mL. What is necessary is just to add so that it may become. The OK432 solution can be prepared by dissolving picibanil (5 KE / tube; Chugai Pharmaceutical Co., Ltd.) with 2 mL of water (for example, water for injection).

  Stimulation with cytokines may be added alone or in combination of a plurality of types. Considering the cost and the like, it is preferable to add IL-2 alone. For example, in the case of IL-2, the amount to be added is preferably in the range of 100 units (U) / mL to 2000 U / mL at the final concentration. If it is less than 100 U / mL, it is insufficient for inducing growth stimulation, and if it is more than 2000 U / mL, NK cell proliferation is not observed as the concentration of IL-2 increases. is there. Preferably, it is in the range of 700 U / mL to 2000 U / mL.

  Further, when the NK cell growth stimulating factor includes an anti-CD3 antibody and / or a bisphosphonate derivative, the anti-CD3 antibody is added to a culture solution containing PBMCs, for example, at a final concentration of 0.01 ng / mL to 1000 ng / mL, preferably 0.1 What is necessary is just to add so that it may become ng / mL-10ng / mL, More preferably, 1ng / mL. When the NK cell growth stimulating factor contains a bisphosphonate derivative or the like, zoledronic acid hydrate injection solution (2.94 μmol / mL; Novartis Pharma) 4 mg / vial may be used as it is. Alternatively, for example, the final concentration may be 1 μM / mL to 10 μM / mL, preferably 3 μM / mL to 7 μM / mL, and more preferably 5 μM / mL.

  After adding each NK cell growth stimulating factor, the blood is preferably kept at a physiological cell temperature (described later) for 1 to 3 days in order to give sufficient stimulation to PBMCs. This period can be performed simultaneously with the next culture step. That is, NK cells and the like can be cultured while applying a stimulus.

  Moreover, you may add a high temperature stimulus in the period for 10 to 30 hours at 38 to 40 degreeC within the holding | maintenance period at the said physiological cell temperature. By this high temperature stimulation, NK cells can be further activated. In addition, if the holding temperature in the stimulation process is lower than 37 ° C, lymphocytes cannot be sufficiently activated, and if it is higher than 40 ° C, the lymphocytes are likely to be denatured or damaged by heat. Therefore, it is not preferable.

The means for maintaining the predetermined temperature is not particularly limited as long as the blood can be maintained at a constant temperature. For example, using a CO ₂ incubator, means for setting a predetermined temperature for each container containing the blood can be mentioned.

1-2-2. Culturing process The “culturing process” is a process of culturing the blood at a physiological cell temperature after the stimulation process. This step is characterized by increasing the number of cells while maintaining the strengthening of NK cells.

  “Physiological cell temperature” refers to an optimum temperature for culturing cells. Usually it is the body temperature of the mammal that provided the blood used. Therefore, when the mammal is a human, it is generally 37 ° C., but may be within a range of less than 0.5 ° C. around the temperature, that is, 36.5 to 37.5 ° C. This is because the temperature in the incubator may fluctuate within the temperature range.

  In this process, initially, it is possible to ensure a period for sufficiently stimulating NK cells with the NK cell proliferation stimulating factor applied in the stimulation process and to culture these cells at the same time. After the stimulation, it is preferable to once remove the NK cell growth stimulating factor from the medium and cancel the stimulation step. Many factors, such as cytokines, can continue to provide enhanced inducing stimulation to NK cells even during the culture process, but can be prolonged over time, such as anti-CD16 antibody, anti-CD137 antibody, OK432, anti-CD3 antibody and / or zoledronic acid This is because stimulation to NK cells may undesirably affect the strengthening of NK cells such as an apoptotic sheath. For example, after removing PBMCs from the culture solution after the stimulation step, the removal method may be transferred to a new culture solution that does not contain anti-CD16 antibody, anti-CD137 antibody and OK432, and further anti-CD3 antibody, zoledronic acid and the like. The removal of the factor and the recovery of PBMCs are achieved by centrifuging the culture solution that has undergone the stimulation step and removing the supernatant. A specific method may be carried out in accordance with the medium exchange method described below.

The culture is performed for 7 to 30 days, preferably 9 to 28 days, 12 to 26 days, or 14 to 24 days in a 5% CO ₂ incubator under physiological cell temperature conditions.

During the culture period, it is desirable to periodically add a new medium or change to a new medium at intervals of 2 to 5 days. As a specific example of medium exchange, first, a culture solution containing NK cells after the stimulation step is transferred to a sterilized centrifuge tube. Subsequently, after centrifuging at about 1200 rpm for 8 minutes at room temperature, the supernatant is removed, or the precipitate containing NK cells is collected. The collected cell precipitate is transferred to a fresh culture medium containing IL-2 and plasma so that the cell density is 0.6 to 1.0 × 10 ⁶ / mL. The cytokine such as IL-2 added at this time may be about 300 U / mL to 700 U / mL at the final concentration. This is because NK cells are already activated after the stimulation step, and NK cells themselves produce cytokines such as IL-2.

  As a medium used for the culture, any general medium used for cell culture can be used in principle. For example, AIM-V medium (Invitrogen), RPMI-1640 medium (Invitrogen), Dulbecco's modified Eagle medium (DMEM; Invitrogen), OpTmizer T-cellExpansion SFM (Invitrogen), TIL (Immuno-Biological Laboratories), epidermis keratinization Examples include cell culture medium (KBM; Kojin Bio Inc.), Iskov medium (IMEM; Invitrogen), Alys medium (Cell Science Research Institute, Inc.), and the like. An OpTmizer medium is preferable.

  After culturing, confirm that there is no bacterial or endotoxin contamination in the culture. The presence or absence of bacteria may be determined by a colony formation assay, and the presence or absence of endotoxin may be determined by a colorimetric method such as a commercially available ELISA or a suspension method such as a Limulus test.

1-3. Effect According to the method for producing an NK cell-enhanced blood product of this embodiment, a blood product in which NK cells are enhanced from blood collected from a living body can be produced.

  According to the method for producing an NK cell-enhanced blood product of this embodiment, a step of maintaining a predetermined temperature at a predetermined temperature, which was an essential step in the previous application (activation step; optional step in the stimulation step of the present invention Is equivalent to high-temperature stimulation). As a result, an incubator set to a predetermined temperature required for the activation process is not necessarily required, so that the burden on the facility and the operation and management of the practitioner at the research facility that implements the present invention is eliminated. It can be greatly reduced.

  Further, according to the production method, blood collected from a living body may be peripheral blood, so that the physical load on the donor can be kept to a minimum.

  According to the production method, there is no need for special dedicated equipment, etc., and equipment that is always available in general laboratory or research facilities that perform cell culture can be used as it is. Easy to obtain. Therefore, if it is a research facility capable of performing aseptic work such as a clean room, there is an advantage that the manufacturing method of the present embodiment can be carried out with almost no initial capital investment.

  According to the NK cell-enhanced blood product obtained by the production method of the present embodiment, it is possible to prevent cancer recurrence and effectively treat advanced cancer in actual clinical experiments. In addition, a safe blood product can be provided such that no side effects are observed in the administration of the blood product.

  Furthermore, when a bisphosphonate derivative is used as one of the NK cell growth stimulating factors, Zometa has been reported to enhance the proliferation activity of γδT cells as described above. Therefore, in addition to the proliferation of NK cells, γδT The effect of cell proliferation can also be expected.

2. 2. NK cell-enhanced blood product 2-1. Outline The second embodiment of the present invention is a NK cell-enhanced blood product obtained by the production method of Embodiment 1.

2-2. Configuration The NK cell-enhanced blood product of the present embodiment can be obtained from the culture solution that has undergone the culture process in the first embodiment. However, the medium used for culture in the NK cell-enhanced blood product and the growth stimulating factor added to the medium are unnecessary. Therefore, when using the NK cell-enhanced blood product, it is preferable to remove the medium and growth stimulating factor from the culture medium as much as possible to prepare enhanced NK cells and the like. As a specific example of the method for removing the culture medium and growth stimulating factor, first, the culture solution containing the strengthened NK cells is transferred to a sterilized centrifuge tube, and centrifuged at 1200 rpm for 8 minutes at room temperature to contain the growth stimulating factor. Remove the supernatant medium. NK cells can be collected as a precipitate. The collected NK cells are preferably washed twice or more with PBS (−). NK cells after washing are counted with a hemocytometer and adjusted with 10 mL to 200 mL of lactated Ringer's solution or physiological saline. Thus, the NK cell-enhanced blood product of this embodiment can be prepared. It is also possible to add cytokines or the like to the blood product as necessary.

  In order to obtain a sufficient effect using the blood product of this embodiment, it is desirable that 70% or more of the number of NK cells contained is in an activated state. Activation of NK cells can be determined by cytotoxic activity using the K562 leukemia cell line or expression of activation markers. As the activation marker, a known marker such as CD69 can be used. Moreover, what is necessary is just to use the antibody with respect to each marker for those detection.

  The NK cell-enhanced blood product of this embodiment can be used immediately after production, or can be used at a temperature of 0 ° C. to 8 ° C. for a predetermined period, or at a super low temperature (about −80 ° C.) by adding a storage solution or the like. It can be stored in liquid nitrogen for a long period of several years. As the preservation solution, it is convenient to use a commercially available lymphocyte preservation solution. For example, bun bunker (Nippon Genetics), CM bunker II (Cosmo Bio) can be used.

2-3. Effect According to the NK cell-enhanced blood product of the present embodiment, 10 × 10 ⁹ to 100 × 10 ⁹ NK cells are included from 20 mL to 60 mL of peripheral whole blood. The number of NK cells in the specimen can be increased rapidly. Therefore, administration of the NK cell-enhanced blood product can enhance the innate immune system of a subject having a disease such as a tumor, and suppress or cure the progression of the disease.

  According to the NK cell-enhanced blood product of this embodiment, a blood product containing a large number of enhanced NK cells can be stored frozen, so that a necessary amount can be administered to a subject when necessary.

3. 3. Composition for enhancing NK cells 3-1. Overview A third embodiment of the present invention is a composition for enhancing NK cells. By adding the composition for enhancing NK cells of this embodiment to a medium containing blood, preferably PBMCs, NK cells in the medium can be easily and efficiently enhanced.

3-2. Configuration “The composition for enhancing NK cells” refers to a composition that can be added to a medium to reinforce NK cells present in the medium.

  The composition for enhancing NK cells of this embodiment is the anti-CD16 antibody, anti-CD137 antibody, OK432 and cytokine described in the first embodiment, or, if necessary, an anti-CD3 antibody, represented by the above formula 1. Bisphosphonate derivatives and the like. The anti-CD16 antibody is preferably an anti-human CD16 monoclonal antibody such as 3G8. The anti-CD137 antibody is preferably an anti-human CD137 monoclonal antibody such as 4-1BB. The cytokine is preferably a compound selected from the group consisting of IL-2, IL-12, IL-15, TNF-α, IL-1β and IL-18, and more preferably IL-2. The anti-CD3 antibody is preferably an anti-human CD3 monoclonal antibody such as muromonab CD3. Further, the bisphosphonate derivative is preferably a compound selected from the group consisting of zoledronic acid, pamidronic acid, alendronic acid, risedronic acid, ipandronic acid, incadronic acid and etidronic acid, and more preferably zoledronic acid. In addition, components of a lymphocyte culture medium such as RPMI-1640, a pH stabilizer, antibiotics, and the like may be included.

  The amount of each constituent component in the composition only needs to be mixed so as to have a predetermined final concentration when added to a predetermined amount of medium. Specifically, the anti-CD16 antibody has a final concentration of 0.01 μg / mL to 100 μg / mL, preferably 0.1 μg / mL to 10 μg / mL, more preferably 1 μg / mL, and OK432 has a final concentration of 0.005 KE. anti-CD137 antibody has a final concentration of 0.1 μg / mL to 10 μg / mL, preferably 0.008 KE / mL to 0.015 KE / mL, more preferably 0.01 KE / mL. 0.3 μg / mL to 6 μg / mL, more preferably 1 μg / mL to 3 μg / mL, and cytokine (preferably IL-2) is 200 U / mL to 2000 U / mL, preferably 700 U / mL to 1500 U / mL, more preferably 1000 U / mL. Furthermore, when an anti-CD3 antibody and / or a bisphosphonate derivative and the like are included, the anti-CD3 antibody (preferably muromonab CD3) has a final concentration of 0.01 ng / mL to 1000 ng / mL, preferably 0.1 ng / mL to 10 ng / mL. More preferably 1 ng / mL, and when a bisphosphonate derivative or the like (preferably zoledronic acid) is included, the final concentration is 1 μM / mL to 10 μM / mL, preferably 3 μM / mL to 7 μM / mL. It may be mixed so that it may be mL, more preferably 5 μM / mL.

  The dosage form of the composition is not particularly limited. A liquid state, a powder state, or a powder dissolved in an appropriate buffer can be tableted by adding an appropriate excipient or the like. Or the mixture of a different state may be sufficient. For example, an anti-CD16 antibody is immobilized on a support such as plastic beads, and OK432, an anti-CD137 antibody and a cytokine, and if necessary, an anti-CD3 antibody and / or a bisphosphonate derivative represented by the above formula 1, The dosage form may be mixed in a solution containing

3-3. Effect According to the composition for enhancing NK cells of the present embodiment, NK cells can be strengthened simply by adding them to a predetermined amount of an appropriate cell culture medium containing NK cells and culturing them.

4). NK cell-enhanced blood production kit 4-1. Outline The fourth embodiment of the present invention is a kit for producing NK cell-enhanced blood. This embodiment can be used when culturing blood, preferably PBMCs, to easily and easily produce a NK cell-enhanced blood product.

4-2. Structure The kit for producing NK cell-enhanced blood according to this embodiment has the anti-CD16 antibody, anti-CD137 antibody, OK432, and cytokine described in the first embodiment, and, if necessary, an anti-CD3 antibody. Bisphosphonate derivative and / or BRM other than OK432. In addition, sterilized water, buffer, instructions for use, etc. can be included to dissolve each NK cell growth stimulating factor.

  The anti-CD16 antibody and anti-CD137 antibody included in this kit, and the anti-CD3 antibody added as necessary may be any antibodies that can specifically recognize and bind to the CD16 antigen, CD137 antigen, and CD3 antigen, respectively. It does not ask | require that it is an antibody or a polyclonal antibody. A monoclonal antibody is preferable. More preferably, the anti-CD16 antibody is immobilized on a suitable support. A specific example of the anti-CD137 antibody is 4-1BB. Furthermore, the cytokine is preferably a compound selected from the group consisting of IL-2, IL-12, IL-15, TNF-α, IL-1β and IL-18, and more preferably IL-2.

  A specific example of the anti-CD3 antibody included in this kit is muromonab CD3 (trade name: Orthoclone OKT3 (registered trademark), Janssen Pharma). Further, the bisphosphonate derivative is preferably a compound selected from the group consisting of zoledronic acid, pamidronic acid, alendronic acid, risedronic acid, ipandronic acid, incadronic acid and etidronic acid, and more preferably zoledronic acid.

  Each NK cell proliferation stimulating factor can be included in the kit alone or in a combination of two or more. For example, NK cell proliferation stimulating factors other than anti-CD16 antibody may be individually packaged and included, or may be included in the kit with some or all of the factors combined. May be. Moreover, the state of each NK cell proliferation stimulating factor is not particularly limited. One NK cell growth stimulating factor may be in a liquid state and the other NK cell growth stimulating factor may be in a solid state. In particular, the anti-CD16 antibody is preferably included in a state of being immobilized on an appropriate support such as plastic beads.

5. 5. Cellular immunotherapy to treat disease 5-1. Outline The fifth embodiment of the present invention relates to a cellular immunotherapy for treating a disease by administering the NK cell-enhanced blood product produced in the first embodiment to a living body to enhance immunity.

5-2. Configuration This embodiment is a cellular immunotherapy in which the NK cell-enhanced blood product obtained by the production method of the first embodiment is administered to a living body.

  “Cellular immunotherapy” as used in this embodiment refers to the treatment of a disease by enhancing the immunity of the living body by administering the NK cell-enhanced blood product obtained by the production method of the first embodiment to the living body. It is a method to do. In particular, the cellular immunotherapy of the present embodiment is preferably premised on adoptive immunotherapy. This is because adoptive immunotherapy has almost no risk of rejection as described above.

  The NK cell-enhanced blood product to be administered is blood containing more lymphocytes having immunity against cancer, viral infection, bacterial infection or parasitic infection than the normal blood average value per unit volume. It is a formulation. “Cancer” as used herein means all malignant tumors. For example, epithelial tumor, sarcoma, leukemia, myeloma and the like are applicable. Specifically, for example, brain tumor, retinoblastoma, basal cell carcinoma, malignant melanoma, tongue cancer, esophageal cancer, stomach cancer, colon cancer, lung cancer, leukemia, lymphoma, breast cancer, cervical cancer, endometrial cancer, ovarian cancer Prostate cancer, testicular cancer, bladder cancer, kidney cancer, liver cancer, pancreatic cancer and fibrosarcoma. In addition, “viral infection” as used herein refers to all diseases caused by viral infections, and particularly applicable to the prevention of chronic viral infections and acute viral infections that are difficult to cure. Examples of such intractable chronic viral infections include HIV infection causing AIDS, viral hepatitis, and human papillomavirus infection causing cervical cancer. Examples of acute viral infections include viral respiratory infections such as influenza and acute viral infections in an immunodeficient state. A “bacterial infection” is an infection caused by eubacteria (including gram-positive bacteria and gram-negative bacteria) or fungi (including filamentous fungi, yeast and basidiomycetes). For example, Candida infection, blastosis, histoplasmosis, etc. are mentioned. Furthermore, “parasite infection” as used herein refers to all diseases caused by protozoa or helminths. For example, malaria, leishmaniasis, filariasis, echinococcosis, Japanese schistosomiasis and the like can be mentioned.

“Immune lymphocyte” means a lymphocyte with enhanced function in the immune system. For example, NK cells, killer T cells, γδ T cells, and NKT cells in a state in which cytotoxic activity is activated are applicable. In addition, the “normal blood average value per unit volume” referred to here is a unit of the number of blood cells having immunity against cancer, viral infection, or fungal infection generally observed in blood of a healthy individual. Mean average value per volume. For example, in the case of NK cells, an average of about 5 × 10 ⁵ NK cells exist per 1 mL of blood of a healthy adult.

5-3. Method The administration method of the NK cell-enhanced blood product in the cellular immunotherapy of the present embodiment will be described below by taking the case of adoptive immunotherapy as an example. The administration method is basically the same as the method known in conventional adoptive immunotherapy except that the NK cell-enhanced blood product of Embodiment 1 is administered. Therefore, the administration method may be carried out according to the administration method in known adoptive immunotherapy. For example, a method of administering the blood product produced by the method for producing the NK cell-enhanced blood product of Embodiment 1 from blood collected from a patient into the body of the patient using intravenous injection or infusion after about 2 weeks Etc.

The dose per administration of the NK cell-enhanced blood product in this embodiment is a volume containing NK cells in the range of 20 × 10 ⁷ to 5 × 10 ⁹ cells in the case of humans. Good. However, this is a general adult dose. In actual administration, it is preferable to appropriately adjust in consideration of the age, sex, weight, disease state, physical strength, etc. of the person who administers the blood product.

  As an example of the cellular immunotherapy in the present embodiment, there is a method in which the administration method is one cycle, and administration is continued for one course (6 cycles) or more at intervals of about two weeks. Even if it is not adoptive immunotherapy, the cell immunotherapy may be performed in the same manner except that an NK cell-enhanced blood product obtained from a living body that is not self is administered.

5-4. Effect According to the cellular immunotherapy of this embodiment, compared with many conventional immunotherapy, especially adoptive immunotherapy, it has high effectiveness in healing with respect to diseases such as cancer. Moreover, since the basic operation technique etc. are the same as the conventional adoptive immunotherapy, it can implement without special technique acquisition if it is a person who has the technique of adoptive immunotherapy.

<Example>
The present invention will be specifically described with reference to the following examples. Note that the following examples merely illustrate the present invention, and the present invention is not limited to these examples. In addition, some experimental errors and deviations are considered with respect to numerical values such as temperature, amount, and time used in this example.

[Example 1]
<Method for producing NK cell-enhanced blood product>
About 1st embodiment of this invention, the specific example about the manufacturing method of the said blood product used by adoptive immunotherapy is given and demonstrated. In the examples of the present specification, a healthy person is used as a donor instead of an original treatment target such as a cancer patient.

(1) Preparation of autologous plasma First, autologous plasma for cell culture was prepared. Heparin was added to the blood collection tube at 50 U / mL, and 40 mL of peripheral whole blood was collected from the donor's vein. The collected peripheral whole blood was transferred to a sterile conical centrifuge tube, centrifuged at 3000 rpm for 10 minutes, and the supernatant was separated as plasma. Preparation of the following PBMCs by adding 3 times the amount of sterile PBS (-) or culture medium to the remaining blood cell components after plasma collection to the whole blood volume before plasma separation. Used for. Plasma was inactivated by treatment at 56 ° C. for 30 minutes, and further centrifuged at 3000 rpm for 10 minutes to remove platelets and the like. Thereafter, the plasma was stored at 4 ° C. The necessary amount was used each time the medium was prepared as autologous plasma for cell culture in which this plasma was added to the medium.

(2) Preparation of PBMCs The erythrocytes and granulocytes were removed using density gradient centrifugation, and PBMCs were separated. Ficoll-Paqu PLUS (Amersham) was used as the specific gravity solution, and the procedure followed the attached protocol. To the collected PBMCs, 30 mL of PBS (-) without serum or a culture medium for cultured cells was added and washed 2 to 3 times. As the culture medium for cultured cells, for example, RPMI1640 medium without serum was used. After washing, a part of the obtained PBMCs turbid solution was sampled, and after tilk staining, the number was counted using a hemocytometer. From 40 mL of peripheral whole blood, 3.4 × 10 ⁷ PBMCs were collected in terms of cell number. The recovered PBMCs were suspended in OpTmizer (Life Techelonogy) medium supplemented with 5% (V / V) autologous plasma so that the cell density was 1 × 10 ⁶ cells / mL.

(3) Stimulation process
0.2 mg of anti-human CD16 antibody (Clone3G8, Beckman Coulter) was dissolved in 1 mL of sterile distilled water. Since this anti-CD16 antibody is not a sterile product, it was sterilized by filtration with a 0.22 μm filter after dissolution. 199 mL was added and mixed with sterile distilled water to a final concentration of 1 μg / mL. After filtration, 5 mL of the anti-CD16 antibody solution was placed in a 25 cm ² culture flask and allowed to stand overnight at 37 ° C. to immobilize the anti-CD16 antibody solution on the inner surface of the flask. Thereafter, the solution was discarded and washed twice with sterile PBS (−).

  5 mL of the prepared suspension of PBMCs was transferred into the flask. Subsequently, 25 μL of anti-human CD137 antibody 0.2 μg / μL solution (4-1BB, BioLegend), 4 μL / mL OK432 (Pisibanil: Chugai Pharmaceutical) aqueous solution 20 μL, and 900 U / μL IL-2 (Proleukin: Chiron) ) 4 μL of the solution was added to the PBMCs suspension and stirred well.

In order to sufficiently stimulate PBMCs with each of the above NK cell growth stimulating factors, the culture flask was transferred to a 5% CO ₂ incubator in which the inside of the chamber was set at 37 ° C. and held for 3 days.

(4) Culture process Then, in order to remove the NK cell growth stimulating factor from the culture solution, 5 mL of the culture solution was collected in a Konica centrifuge tube and centrifuged at 1200 rpm for 8 minutes. After centrifugation, the supernatant medium is removed and the cell pellet is recovered by suspending in 4 mL of OpTmizer medium containing 5% (V / V) autologous plasma containing 700 U / μL IL-2. After transferring to a new flask in which the antibody was not immobilized, it was cultured again in a 5% CO ₂ incubator set at 37 ° C. for 21 days. The OpTmizer medium containing 5% (V / V) autologous plasma was changed every 2-4 days. As described above, the NK cell-enhanced blood product of the second embodiment of the present invention was produced.

  In fact, when used as an NK cell-enhanced blood product, it is necessary to perform a contamination test and pretreatment. These will be briefly described below.

(5) (Contamination test)
Verification of the presence or absence of endotoxin in the culture solution was performed using Limulus ES-II (Wako) according to the attached protocol. The presence or absence of bacteria or mold was confirmed by applying a part of the culture solution to an agar medium and using a colony formation assay.

(NK cell-enhanced blood product pretreatment)
The culture solution for 3 weeks after culturing was transferred to a centrifuge tube, centrifuged at 1200 rpm for 10 minutes, and the supernatant was discarded. 50 mL of PBS (-) was added to suspend the precipitate, and the mixture was centrifuged again at 1200 rpm for 10 minutes, and then the supernatant was discarded. This operation was repeated three times to remove the medium components. Finally, it was suspended in 70 mL of lactated Ringer's solution. By the above operation, the final product, NK cell-enhanced blood product, was obtained. With this blood product, the NK cell proliferation rate was about 16000 times after 14 days and about 44000 times after 21 days. This was slightly more than 4 times after 14 days and 21 days compared with the NK cell proliferation rate obtained by the conventional method for producing NK cell-enhanced blood products using only anti-CD16 antibody, IL-2 and OK432. .

[Example 2]
<Proliferation of NK cells (1)>
In order to confirm that the production method of the NK cell-enhanced blood product of the present invention does not require high temperature stimulation, the proliferation rate of NK cells was verified.

(Method)
In this example, a method for producing a NK cell-enhanced blood product of the present invention using an anti-CD16 antibody, an anti-CD137 antibody, an NK cell growth stimulating factor comprising OK432 and IL-2, and an anti-CD137 antibody against the NK cell proliferation stimulating factor In order to compare the results obtained in each of the same methods as the method for producing the NK cell-enhanced blood product of the present invention except for not using, healthy donors who obtained informed consent for the following two samples The proliferation rate of NK cells in blood obtained from the above was verified.
Sample a: As a NK cell proliferation stimulating factor, 1 μg / mL anti-CD16 antibody, 0.01 KE / mL OK432 and 700 U / mL IL-2 were used at final concentrations, respectively. The NK cell growth stimulating factor in this sample corresponds to the growth stimulating factor used in the previous application.
Sample b: As a NK cell growth stimulating factor, final concentration of 1 μg / mL anti-CD16 antibody, 0.01 KE / mL OK432, final concentration of 1 μg / mL anti-CD137 antibody solution (4-1BB, BioLegend) and 700 U / mL IL-2 was used. The NK cell proliferation stimulating factor in this sample corresponds to the NK cell proliferation stimulating factor of the present invention.

  Further, unlike the manufacturing method according to the previous application, the samples a and b are not subjected to a high temperature stimulation process at 39 ° C.

The basic operation of the method for producing an NK cell-enhanced blood product is the same as in Example 1 except for the difference in the composition and process of each sample. The day when the PBMCs were suspended in OpTmizer medium so that the cell density would be 1 × 10 ⁶ cells / mL was defined as day 0. Stimulation with each stimulating factor was given, and 10% autologous plasma was added to the medium for stimulation and culture. The day when this was started was defined as day 0. A part of the culture solution was collected on days 3, 5, 7, 10, 12, 14, 17, and 21 of the culture, and the total number of cells in each culture solution was measured.

  Measurement of NK cells in the culture broth was performed on day 0, day 14 and day 21 using flow cytometry analysis. Specifically, NK cells in blood products are combined with a fluorescent antibody-labeled monoclonal antibody (PC5 label or ECD label-anti-CD3 antibody, PE label or PC5 label-anti-CD56 antibody Immunotech). Immunostained. Immunostaining was performed by adding the antibody amount recommended in the package insert of each antibody to the cell suspension, staining at room temperature for 15 minutes, and then centrifuging to wash away the supernatant containing the fluorescent antibody. Subsequently, the kinetics of NK cells were measured by flow cytometry using Cytomic FC500 (Beckman) with the above antibody combination. The measured data was analyzed by CXP analysis.

(result)
The results are shown in FIGS. 1 and 2 and Table 1.

As shown in FIG. 1, the% of NK cells (B1; CD3 ⁻ CD56 ⁺ ) in the total number of cells was not significantly different between samples a and b on day 0, 14 and 21.

  However, as shown in FIG. 2, a difference in the total cell number began to occur between samples a and b from around day 13 after the start of culture. As shown in Table 1, on the 14th day, the absolute number of NK cells in sample b reached about 4 times that in sample a, as shown in Table 1. The NK cell-enhanced blood product used in the present embodiment, when compared with the same liquid volume as the blood volume used for the culture, (total number of cells at the end of culture × ratio of NK cells) / (PBMCs at the start of culture × From the ratio of NK cells, the number of NK cells was found to be 10,000 times or more than the average value of each blood cell number per unit volume. Therefore, according to the production method of the present invention, it has been clarified that NK cells can be proliferated more efficiently without requiring high temperature stimulation.

[Example 3]
<Measurement of activated NK cells>
The activation of NK cells in the NK cell-enhanced blood product of the present invention was measured as cytotoxic activity against the K562 cell line, which is the target of NK cells.

(Method)
First, K562 cells, which are leukemic cell lines, were labeled with the fluorescent dye Calcein-AM. Labeling was performed by adding 1/100 volume of Calcein-AM solution (Dojindo Laboratories) to RPMI-1640 medium (containing 10% fetal bovine serum) and incubating at 37 ° C. for 30 minutes. After labeling, the cells were washed with PBS (−) and used as target 562 cells. Next, NK cells in the NK cell-enhanced blood products derived from samples a and b produced by the method of Example 2 were used as effector cells (E), respectively, and the ratio to target K562 cells (target cells: T) ( (E / T ratio) was adjusted to a predetermined value, and then each was placed in a 96-well plate and reacted at 37 ° C. with a CO ₂ concentration of 5% for 2 hours. After the reaction, the amount of target cells retaining fluorescence, that is, surviving, was detected based on the fluorescence intensity using Terascan VP (Minervatech). The cytotoxic activity value of K562 was calculated by comparison with the control before injury, that is, the fluorescence intensity in the state where no effector cells were added.

(result)
The cytotoxic activity of the NK cells derived from the samples a and b produced by the method of Example 2 is shown in Table 1, and the cytotoxic activity of the NK cells derived from the samples a and b on the 14th and 21st days is shown in FIG. 3 respectively. The E / T ratio used is shown in each table or figure.

  From Table 1 and FIG. 3, the cytotoxic activity of the NK cells obtained by the method for producing the NK cell-enhanced blood product of the present invention and the method for producing the NK cell-enhanced blood product according to the previous application are almost the same. It became clear that there was no. Furthermore, even after culturing up to the 21st day, the cytotoxic activity did not decrease. Therefore, the production method of the NK cell-enhanced blood product of the present invention can obtain more activated NK cells while maintaining the cytotoxic activity of the NK cells obtained by the production method according to the previous application. It became clear that we could do it.

Claims (6)

A stimulating step of stimulating NK cells contained in blood collected from a living body with NK cell proliferation stimulating factors including anti-CD16 antibody, OK432, anti-CD137 antibody, and IL-2, and physiological cells after the stimulating step A method for producing an NK cell-enhanced blood product comprising a culture step of culturing at a temperature.   The manufacturing method of Claim 1 whose physiological cell temperature is 36.5-37.5 degreeC.   The manufacturing method of Claim 1 or 2 whose culture | cultivation periods in a culture | cultivation process are 7 days-30 days.   The production method according to any one of claims 1 to 3, wherein the anti-CD16 antibody is immobilized on a support.   A composition for enhancing NK cells, comprising anti-CD16 antibody, OK432, anti-CD137 antibody and IL-2. A kit for producing NK cell-enhanced blood comprising the composition for enhancing NK cell according to claim 5 .

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