JP5542379B2 - Stirring device, stirring method, and automatic gene testing device - Google Patents

Description

  The present invention relates to a method and apparatus for stirring a small amount of a reaction solution in a non-contact manner.

  Conventional methods for stirring reaction solutions in biochemical analysis can be largely classified into contact stirring and non-contact stirring. In the case of contact stirring, a method using a stirring bar and a method using suction and discharge using a syringe mechanism or the like are mainly used. In the method using a stirring rod, the tip of the stirring rod is inserted into the reaction solution, and the stirring rod is rotated to stir the solution. After the solution is stirred, the tip of the stirring bar is washed to prevent carryover to other samples. This system can stir effectively the solution which is hard to stir by devising the tip shape. Moreover, the method by suction discharge using a syringe mechanism etc. stirs a solution by inserting the tip of a dispensing probe or a dispensing tip into a reaction solution, and sucking and discharging the reaction solution. When a dispensing probe is used after the solution is stirred, the tip and inner wall of the dispensing probe are washed to prevent carryover. Moreover, when the disposable dispensing tip is used, the used dispensing tip is discarded.

In the case of non-contact stirring, there are mainly a stirring method by fixing the reaction vessel and applying ultrasonic waves to the reaction solution, and a stirring method using a mechanism for moving the reaction solution vigorously with the reaction vessel. In the stirring method using ultrasonic waves, the reaction solution was vigorously moved and stirred effectively by filling the periphery of the reaction vessel with a liquid and applying ultrasonic waves to the reaction liquid through the surrounding liquid. In the method using a mechanism for moving the reaction vessel vigorously, the reaction vessel is placed on the stirring mechanism and the stirring mechanism is operated to perform stirring. More specifically, according to the stirring mechanism of Patent Document 1, stirring was performed by fixing the top of the reaction vessel and rotating the reaction vessel bottom eccentrically. In addition, according to the stirring mechanism implemented in the automatic immunoanalyzer (Roche), stirring was carried out by fixing the middle of the reaction vessel and rotating the bottom of the reaction vessel and the upper portion of the reaction vessel.

  In particular, as a test that requires a small amount of reaction solution and a non-contact stirring method, for example, a genetic test in which viral nucleic acid is amplified and detected can be mentioned. The genetic test is theoretically a test method that can be detected by exponentially amplifying even one molecule of viral nucleic acid, and there are many analytical methods such as the LAMP method, NASBA method, and TMA method including the PCR method. . The characteristics of these analytical methods are that the amount of reaction solution is very small, the shape of the reaction vessel is special because it is very small, and the risk of carryover is high because of its very high sensitivity. Since exponential amplification starts after the solution is prepared, even a small amount of reaction solution is a risk of false positives. Therefore, in the conventional genetic testing by hand, non-contact stirring is performed by combining a vortex stirring and a centrifuge as a stirring method with a reaction container corresponding to a trace amount solution with a low risk of carryover. More specifically, vortex agitation is an agitation mechanism that moves up and down at the same time as eccentric rotation. Effective agitation is performed by scattering the reaction solution throughout the reaction vessel and collecting the solution with a centrifuge. At this time, stirring was possible even in a reaction vessel that had a narrow tip of the reaction vessel and was difficult to stir by a stirring method using a stirring mechanism.

  In the case of a reaction solution containing an enzyme that tends to lose its activity due to vigorous stirring, the stirring method using the mechanical vortex mixer described above is not performed, and stirring is performed by tapping operation by stirring the reaction container by hand. Was.

JP 2002-255 A

  In the contact stirring, when the reaction vessel has a lid, a lid opening / closing process is required, and the automatic analyzer has a problem that an additional mechanism is necessary. In addition, a washing process is required by a method using a stirring bar or a method using a dispensing probe, and an automatic analyzer requires an additional mechanism and operation. Further, in this washing step, in the case of a genetic test that can be detected from one copy of the target nucleic acid, there is a problem that washing is difficult to prevent carryover, and there is a problem that causes false positives. In addition, when the amount of the reaction solution is very small, a change in the amount of the reaction solution caused by bringing the cleaning solution adhering to the tip of the stirring rod into the reaction system at the time of washing affects the analytical reaction. In addition, in the case of the stirring method using suction and discharge using a dispensing tip, the reaction solution containing the sample adheres to the tip of the dispensing tip, so the risk of sample contamination during disposal is great.

  When ultrasonic stirring is used as non-contact stirring, it is necessary to fill the periphery of the reaction vessel with a liquid, and application is difficult when a liquid is not used for reaction temperature control. In addition, since ultrasonic waves generate a large amount of bubbles, there is a problem that the generated bubbles affect the detection in the case of a trace reaction and detection. In addition, in the stirrer having an inclined rotation axis as applied in the automatic immune analyzer (e170, Roche) shown in Patent Document 1, the bottom shape of the reaction vessel is extremely narrowed to cope with a small amount of liquid. Sometimes, the liquid at the bottom of the reaction vessel is difficult to move in the stirring operation by the pendulum motion of the reaction vessel, and stirring is difficult. In particular, in the configuration shown in Patent Document 1, that is, when the upper end of the reaction vessel is fixed and the reaction vessel bottom is decentered, the liquid at the bottom of the reaction vessel remains difficult to move even if the rotational speed and the eccentric distance are increased, There was a problem that the liquid at the bottom of the container could not be completely stirred. In addition, when a plurality of reaction liquids having different viscosities are sequentially stirred, the ease of stirring varies depending on the difference in viscosity, and thus there is a problem that stirring is difficult in the single stirring operation disclosed in Patent Document 1.

  In addition, in a device that automates a minute reaction of 100 μl or less such as genetic testing, a specially shaped container with a narrow reaction container bottom is required. As the stirring process using the container, there are problems such as preventing carry-over, suppressing the generation of bubbles, reliably stirring the liquid at the bottom of a narrow container, and spreading the reaction liquid aerosol after stirring the dispensing tip, resulting in high contamination risk It was difficult to carry out stirring in a form that solved all of the above. Furthermore, in genetic testing, there are many enzymes that are easily inactivated by vigorous stirring, and there has been a problem that reactive enzymes are inactivated by conventional vigorous vortexing. Further, when it is necessary to control to a high temperature during pretreatment or reaction, mineral oil is added to form a mineral oil film on the upper layer of the reaction solution to prevent evaporation. In the case of such an analysis method, if vigorous stirring is performed, the mineral oil adheres to the bottom of the container due to its high viscosity, so that there is a problem that the reaction detection from the bottom of the container becomes poor. In addition, when a plurality of analysis items having different viscosities are requested as a genetic test apparatus, it is required that reaction liquids having different viscosities can be sequentially stirred, but stirring is performed only by a single operation shown in Patent Document 1. In this case, it was difficult to deal with all analysis items. In addition, in the case of constant temperature amplification such as LAMP, NASBA, and TMA, amplification starts when the enzyme is added, and therefore the amplification reaction is affected when the temperature is extremely high or low during stirring after addition of the enzyme. There was a problem to do.

  It comprises at least an erection base for erection of the reaction vessel, a rotation drive body for rotating the erection table, and a control unit for controlling the rotation of the rotation drive body, and the center of the reaction vessel installation part of the erection base is the rotation center of the rotation drive body Using a stirring mechanism characterized in that the rotation axis of the reaction vessel is vertical, and the control unit repeatedly performs high-speed rotation, slow-speed rotation, or rotation stop on the installation base. Features device.

  In addition, a temperature control mechanism is provided around the stirring mechanism and the stirring mechanism, and a transport mechanism for laying the reaction container, a dispensing mechanism for adding the reaction liquid to the reaction container, and a detector for detecting the reaction are provided. A genetic testing device in which a reaction vessel is placed in a stirring mechanism by a transport mechanism after the reaction solution is added to the container, and stirred by the stirring device described above while being maintained at a predetermined temperature by a temperature control mechanism. In addition, the genetic testing device that switches the control operation of the stirring control unit according to the analysis item when the viscosity of the reagent varies depending on the analysis item.

  By carrying out the present invention, stirring can be performed without contact, and carry-over can be prevented. Furthermore, since stirring is possible with the container completely sealed, scattering of the reaction solution by the stirring rod or stirring tip during and after stirring can be completely prevented. Furthermore, even a minute container having a narrow container bottom can efficiently stir the reaction solution at the bottom of the reaction container. Furthermore, a plurality of reaction liquids having different viscosities can be continuously stirred. Further, even when mineral oil is present in the upper layer of the reaction solution, the mineral oil can be stirred without contacting the bottom of the container. Furthermore, since this is an agitation method that automatically realizes the agitation by tapping that has been realized only by the conventional method, it can be agitated while suppressing inactivation against an enzyme that is easily inactivated by vigorous agitation such as vortex. With this stirring mechanism and a temperature control mechanism installed around the stirring mechanism, automatic stirring can be realized even in genetic tests that are sensitive to temperature, highly sensitive, and have a high risk of contamination.

It is a figure which shows the outline of the stirring apparatus of this invention, (A) has one container installation hole, (B) has a plurality of container installation holes. (C) is a figure which shows the mode of the reaction liquid in the container at the time of rotation stop / slow-speed rotation and high-speed rotation. It is a figure which shows the outline of the stirring apparatus of this invention. It is a figure which shows the processing flow of a user and a system. It is a block diagram which shows the outline of the gene automatic test | inspection apparatus of this invention. It is a top view which shows schematic structure of a genetic test | inspection apparatus. It is the figure which added the movement mechanism to the genetic test | inspection apparatus shown in FIG. FIG. 6 is a diagram of the genetic testing device and the external device shown in FIG. 5.

  One of the best modes of the present invention is comprised of at least a erection base for erection of a reaction vessel, a rotary drive member for rotating the erection stand, and a control unit for controlling the rotation of the rotary drive member. The center of the unit is at a position different from the center of rotation of the rotary drive body, and the rotation axis of the reaction vessel is vertical. A stirrer characterized by repeatedly performing slow rotation or rotation stop.

  Another best mode of the present invention includes at least a dispensing mechanism, a reaction container transport mechanism, a stirring mechanism, a detection mechanism, and a control unit, and the stirring mechanism has the above-described configuration and stirring method. Device. Preferably, when the temperature change during the stirring affects the test result, the temperature control mechanism is provided around the stirring mechanism, and more preferably, when the viscosity of the reagent varies depending on the analysis item, depending on the analysis item A genetic test apparatus characterized by switching a control operation of a stirring control unit.

  The present invention has resulted in the present invention as a result of stirring the reaction container and various studies on the micro container.

  Hereinafter, an apparatus configuration and an operation method for realizing the present invention will be described in detail with reference to the drawings to which the present invention is applied. The present invention relates to a mounting base for mounting a reaction vessel, a rotary driving body for rotating the mounting base, and Consists of a control unit that controls the rotation of the rotary drive body, the center of the reaction vessel installation portion of the gantry is at a position different from the rotation center of the rotary drive body, and the rotation axis of the reaction vessel is vertical As long as the control unit can repeatedly perform high-speed rotation, slow-speed rotation, and rotation stop alternately, the invention is not limited to the specific examples described below.

  The minimum mechanism and configuration required for the present invention are shown in FIGS. As shown in FIGS. 1 (A) and 1 (B), the present invention comprises at least a erection base on which a reaction vessel is erected, a rotary driving body that rotates the erection base, and a control unit that controls the rotation of the rotary driving body. The center of the reaction vessel installation part of the base is composed of a stirring mechanism characterized in that the center of the reaction vessel is at a position different from the rotation center of the rotary drive body and the rotation axis of the reaction vessel is vertical. More preferably, the installation table and the rotary drive body are connected by a connection mechanism, and a large number of reaction vessels can be installed on the installation table.

  The reaction vessel in the present invention is not limited to a reaction vessel as long as it encloses a reaction solution to be stirred. However, particularly when a reaction vessel having a narrow vessel bottom is used, one of the characteristic effects of the present invention is provided. Thus, effective stirring of the reaction liquid at the bottom of the reaction vessel can be performed. The reaction container having a narrow container bottom means a width of 3 mm or less at the bottom of the container, and specifically, it is the bottom shape of a PCR tube for molecular biology, but the present invention is not limited to this specific example. . The “reaction solution” in the present invention may be any reagent that is conventionally used in general, such as biochemical tests and genetic tests. The present invention is particularly useful when stirring an enzyme that tends to be inactivated by vigorous stirring. Stirring that prevents enzyme deactivation, which is one of the characteristic effects, can be performed. Moreover, even if the mineral oil for preventing evaporation is added to the upper layer of the reaction liquid, the present invention enables effective stirring.

  As shown in FIGS. 1 (A) and 1 (B), the installation base in the present invention has at least one hole in which a reaction vessel can be installed, and the vertical axis of the reaction vessel is not inclined by a stirring operation. To do. More preferably, a large number of reaction vessels can be installed, or a reaction plate having a plurality of holes can be installed. More specifically, a PCR tube can be installed vertically or a 96-hole PCR plate can be installed.

  The rotational drive body in the present invention can rotate the installation base at a constant speed, and preferably has a variable speed and rotation direction, and any mechanism may be used as the drive mechanism. More preferably, a stepping motor is used. As a control part which controls a rotational drive body, what kind of structure may be sufficient if the structure shown in FIG. 2 is controllable.

  As a stirring operation method for realizing the present invention, high-speed rotation for biasing the reaction solution to one side in the reaction vessel and slow-speed rotation or stopping rotation for returning the reaction solution in the reaction vessel to a stationary state are alternately repeated. Then, the reaction solution is largely moved to the reaction solution at the bottom of the reaction vessel and stirred. More preferably, when the viscosity of the reaction liquid increases, the number of times of repeating high-speed rotation and slow-speed rotation or rotation stop and / or the rotation speed of high-speed rotation increases.

  The high-speed rotation in the present invention means a rotation in which the reaction solution extends to the inner wall of the reaction vessel by the centrifugal force of rotation around the rotation center. This rotational speed is inversely proportional to the eccentric distance, and if the eccentric distance is large, the rotational speed decreases, while the stirring mechanism also has a disadvantage that the size of the stirring mechanism increases. Can be set to any setting for the rotational speed and the eccentric distance. In the present invention, “the state in which the reaction solution extends to the inner wall of the reaction vessel” means that the lowest point of the liquid level during high-speed rotation is not more than half of the liquid level before rotation, and is limited to the rotation speed and the eccentric distance. Not. The eccentric distance in the present invention means an amplitude distance of rotation. More specifically, in the case of the configuration shown in FIGS. 1A and 1B, it means the distance between the center of the reaction vessel and the center of rotation.

  The slow rotation or rotation stop in the present invention is not limited to the rotation speed of the rotary drive body as long as the liquid level of the reaction liquid can be made horizontal. The operation of alternately repeating high-speed rotation and slow-speed rotation or rotation stop in the present invention is only required to be able to alternately switch between high-speed rotation and slow-speed rotation or rotation stop, and is limited to the length of high-speed rotation time, slow-speed rotation time, or rotation stop time. The speed of the high speed rotation repeated here may be different at each time or the same speed. More preferably, the shorter the slow rotation time or the rotation stop time, the better in order to shorten the stirring time. If the viscosity of the reaction solution is high, effective stirring can be achieved by increasing the speed or time of high-speed rotation or the eccentric distance and increasing the number of repetitions of high-speed rotation, slow-speed rotation, or rotation stop.

  As another embodiment of the present invention, as shown in FIG. 5, FIG. 6, and FIG. 7, it has at least a dispensing mechanism, a reaction container transport mechanism, a stirring mechanism, a detection mechanism, and a control unit. It is a genetic test apparatus characterized by being. In the case where the temperature change during stirring in the present invention affects the test result, the temperature control mechanism is provided around the stirring mechanism, and the stirring mechanism has a liquid property of the reagent used according to the requested test. When changing, the stirring method may be changed according to the requested inspection. Preferably, the stirring mechanism has a mechanism for covering the reaction vessel before stirring.

  The genetic test in the present invention is not limited to the genetic test method as long as a specific gene can be amplified and specifically detected. Specific examples include Polymerase chain reaction (PCR method), Nucleic Acid Sequencing Based Amplification method (NASBA method), LAMP method, TMA method, SDA, etc. The present invention can amplify and detect specific genes. However, the present invention is not limited to these specific examples.

  The dispensing mechanism in the present invention is not limited to a dispensing method and a dispensing method as long as it can dispense a reaction solution used for genetic testing with an accuracy required for analysis. Preferably, 50 μl or less can be dispensed, and more preferably, 10 μl or less can be dispensed. The transport mechanism in the present invention may be any mechanism as long as the reaction vessel can be installed in the stirring mechanism. As a more specific example, there is a transport mechanism that can hold the reaction container, lift it up and transport it, and lower the reaction container to lower the reaction container so that the reaction container can be installed on the mounting base of the stirring mechanism. It is not limited to. The detection mechanism in the present invention may be any detection mechanism as long as the detection reaction of the reaction liquid in the reaction vessel can be detected. More specifically, when a nucleic acid amplification reaction is detected using a fluorescent dye, a fluorescence detector is used as a detection mechanism. The temperature adjusting mechanism provided around the stirring mechanism may be controllable at the temperature required for the detection reaction. More preferably, some genetic testing methods such as NASBA method, LAMP method, and TMA method require a temperature control mechanism for the pretreatment reaction, and therefore the heat of the temperature control mechanism is used for temperature control during stirring.

  Examples of the present invention will be described in detail below, but the present invention is not limited to these examples.

  The stirring of the micro reaction container by the stirring unit will be described.

(1. About reagent and consumables composition)
Table 1 shows a list of laboratory instruments and reagents used in this example.

(2. Device configuration in the present embodiment)
The structure of the stirring mechanism is shown in FIG. Table 3 shows the configuration of the control unit.

(3. Implementation method)
Table 4 below shows the procedure for carrying out the present invention, Table 5 shows stepping motor setting values such as stirring speed and time during stirring, and Table 6 shows detailed stirring control.

  In Table 6, an alphabetic character means the number of repetitions of the operation in parentheses immediately after, and each alphabetic character corresponds to Table 9.

  Also, it was investigated whether stirring can be completed quickly even with a highly viscous solution by making the stirring operation more complicated. In this examination, the stirring operation shown in Table 7 was performed. Table 7 shows the high-speed stirring operation for high viscosity.

(4. Implementation results)
Table 8 below shows the experimental results obtained by visual confirmation when stirring was performed with the samples and apparatus configurations shown in Tables 1 and 2. Table 8 shows the results of examining the stirring effect.

  In Table 8, OK indicates good stirring, and NG indicates poor stirring.

  From the above results, the stirring effect can be increased by increasing the number of repetitions of high-speed rotation and rotation stop due to the difference in viscosity. From these results, the values of X, Y, and Z shown in Table 6 were set as shown in Table 9. Table 9 shows the detailed stirring operation.

  Moreover, the stirring operation was examined to make it possible to complete the stirring quickly even with a highly viscous solution by making the stirring operation more complicated. As a result of this examination, stirring was possible with the number of stirrings shown in Table 10. Table 10 shows the agitation study results.

  From this result, it is possible to prevent carry-over by stirring without contact, and at the same time, it is possible to stir with the container completely sealed, so that the reaction by a stirring rod or a stirring tip during and / or after stirring. Liquid splash can be completely prevented. Furthermore, even a minute container having a narrow container bottom can efficiently stir the reaction solution at the bottom of the reaction container. Furthermore, a plurality of reaction liquids having different viscosities can be continuously stirred. Further, even when mineral oil is present in the upper layer of the reaction solution, the mineral oil can be stirred without contacting the bottom of the container. Furthermore, since this is an agitation method that automatically realizes the agitation by tapping that has been realized only by the conventional method, it can be agitated while suppressing inactivation against an enzyme that is easily inactivated by vigorous agitation such as vortex.

Genetic testing device with stirring mechanism (1. Measurement conditions)
Table 11 shows the measurement conditions of the NASBA method, which is one of the nucleic acid analysis methods, Table 12 shows the NASBA method reagents and samples used in the present invention, and Table 13 shows the sample preparation method using the reagents of Table 12.

(2. Device configuration)
Table 14 shows the apparatus configuration necessary for implementing the NASBA method by applying the present invention.

(3. Inspection)
FIG. 3 shows a test flow of the genetic test system for realizing the present invention. In FIG. 3, “·” indicates “and”, and “/” indicates “and / or”. By applying the present invention to the genetic test shown in this example, the reaction solution can be stirred without the dispensing tip coming into contact with the reaction solution, and the amplified nucleic acid becomes an aerosol from the discarded dispensing tip. The risk of spreading and adversely affecting analysis results can be reduced. In addition, by adding temperature control to the stirring mechanism, if the temperature drop during stirring affects the analysis result, the temperature drop can be prevented. FIG. 4 shows a flow of determining and executing the stirring operation of the system when the stirring operation needs to be changed depending on the liquid property of the type of the inspection item (reagent). According to this flow, it is shown that the optimum stirring operation is determined by the analysis item, and the inspection system is automatically executed. This stirring operation differs depending on the liquidity of the reagent relative to the analysis item, and does not define a specific stirring operation. With this function, an optimal stirring operation can be executed for each analysis item.

1 Sample container 2 Sample container rack 3 Reagent container 4 Reagent rack 5 Reaction container 6 Reaction container rack 7 Dispensing tip 8 Dispensing tip rack 9 Incubator 10 Reaction container carrier 11 Reaction disk 12 Detector 13 Stirrer 20 Dispensing probe 21 Reaction vessel transfer mechanism 22 Head 23 Side rail 24 Center rail 30 Storage device 31 Computer 32 Monitor

Claims (17)

A mounting base capable of vertically mounting the container, a rotation driving body that rotates the mounting base, and a control unit that controls the rotation of the rotation driving body;
The center of the container mounting portion of the installation base is at a position different from the rotation center of the rotary drive body, and is configured so that the vertical axis of the container does not tilt when rotating,
Before SL controller, stirring device which comprises carrying out alternately and stop high-speed rotation and slow rotation or rotation. A mounting base capable of vertically mounting the container, a rotation driving body that rotates the mounting base, and a control unit that controls the rotation of the rotation driving body, and the center of the container mounting portion of the mounting base is the center of the rotation driving body At a different position from the center of rotation,
The control unit alternately repeats the first high-speed rotation and rotation stop, and the second high-speed rotation and rotation stop repetition,
The stirring device characterized in that the speed of the first high-speed rotation is different from the speed of the second high-speed rotation. A stirrer according to claim 1 or 2 ,
The stirrer characterized in that the viscosity of the reaction liquid in the container is directly proportional to the number of times of high-speed rotation and slow-speed rotation or repeated rotation stoppage. The stirring device according to any one of claims 1 to 3 ,
The stirrer characterized in that the viscosity of the reaction liquid in the vessel is directly proportional to the speed during the high-speed rotation. A stirrer according to claim 1 or 2 ,
The agitation device, wherein the installation base and the rotary drive body are connected by a connecting portion, and the entire installation base rotates eccentrically by the connection portion. A stirrer according to claim 1 or 2 ,
The stirrer is characterized in that the erection base is constructed with a container having a bottom width of 3 mm or less. A stirrer according to claim 1 or 2 ,
A stirring device comprising a temperature adjusting mechanism for adjusting the temperature of a container. Repeat the first high-speed rotation and rotation stop and the second high-speed rotation and rotation stop at a position different from the rotation center for rotating the installation portion at the center of the container installation portion in the installation base for laying the container The stirring method is characterized in that the first high-speed rotation speed and the second high-speed rotation speed are different . The stirring method according to claim 8, wherein
The viscosity of the reaction liquid in the container, the number of repetitions of the first high-speed rotation and rotation stop, and / or the number of repetitions of the second high-speed rotation and rotation stop, and / or the number of times of alternately performing A stirring method characterized by being directly proportional. A stirring method according to claim 8 or 9, wherein
The stirring method, wherein the viscosity of the reaction liquid in the container is directly proportional to the speed during the high-speed rotation. A mounting base capable of vertically mounting the container, a rotation driving body that rotates the mounting base, and a control unit that controls the rotation of the rotation driving body;
The center of the container mounting portion of the installation base is at a position different from the rotation center of the rotary drive body, and is configured so that the vertical axis of the container does not tilt when rotating,
A stirring mechanism before Symbol controller is carried out alternately and stop high-speed rotation and slow rotation or rotation,
A dispensing mechanism for adjusting the reaction solution;
A transport mechanism for transporting the reaction vessel;
An automatic gene testing apparatus comprising a detector for detecting a reaction in a reaction solution.   A mounting base capable of vertically mounting the container, a rotation driving body that rotates the mounting base, and a control unit that controls the rotation of the rotation driving body;
The center of the container mounting portion of the installation base is at a position different from the rotation center of the rotary drive body,
The control unit alternately repeats the first high-speed rotation and rotation stop, and the second high-speed rotation and rotation stop repetition,
The speed of the first high-speed rotation is different from the speed of the second high-speed rotation.
A stirring mechanism;
  A dispensing mechanism for adjusting the reaction solution;
  A transport mechanism for transporting the reaction vessel;
  An automatic gene testing apparatus comprising a detector for detecting a reaction in a reaction solution. The gene automatic testing device according to claim 11 or 12 ,
An automatic gene testing apparatus, wherein a stirring method is changed according to an assay item when an assay request is confirmed. The gene automatic testing device according to claim 13 ,
An automatic genetic testing apparatus characterized in that the viscosity of a reaction solution used for an assay item at the time of assay request determination is directly proportional to the number of times of repeated high-speed rotation and slow-speed rotation or rotation stoppage. The gene automatic testing device according to claim 13 or 14 ,
An automatic gene testing apparatus characterized in that the viscosity of a reaction solution used for an assay item when an assay request is confirmed is directly proportional to the speed at the time of high-speed rotation. The gene automatic testing device according to claim 11 or 12 ,
The automatic gantry testing apparatus characterized in that the erection base erected a container having a bottom width of 3 mm or less. The gene automatic testing device according to claim 11 or 12 ,
An automatic gene testing apparatus comprising a temperature adjusting mechanism for adjusting the temperature of a container.

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