JP5376428B2 - Analytical device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an analyzing device capable of displaying the quantitative suction of a sample solution. <P>SOLUTION: The opening part 101 of a supplying capillary flow channel 11 is opened at the leading end of a spotted part 103, the supplying capillary flow channel 11 is connected to a reagent chamber 4 through a holding chamber 3, a protruded part 3b for forming a filling confirming region 3a having a smaller gap (0.1 mm) is formed to the terminal part of a holding chamber 3, so that the arrival of the sample solution at the gap of the filling confirming region 3a can be confirmed from a confirming window 20 at the time of completion of the quantitative sampling of the sample solution. <P>COPYRIGHT: (C)2010,JPO&amp;INPIT

Description

  The present invention relates to an analytical device used for analyzing a liquid collected from a living organism.

Conventionally, as a method for analyzing a liquid collected from a living organism or the like, a method for analyzing using a device for analysis in which a liquid channel is formed is known. The analytical device can control the fluid using a rotating device, and utilizes centrifugal force to dilute the sample liquid, measure the solution, separate the solid component, transfer and distribute the separated fluid, Since a solution and a reagent can be mixed, various biochemical analyzes can be performed.
JP 7-500910 (Fig. 1)

  When the analysis device is classified according to the method of taking the sample liquid, in addition to the type in which an appropriate amount is injected by a syringe as seen in Patent Document 1, the opening 101 of the capillary channel is used as a sample liquid reservoir as shown in FIG. A type that is brought into contact and sucked up by capillary force is conceivable.

  In the analysis device 100 shown in FIG. 26, an opening 101 is provided in a spotting portion 103 formed so as to protrude from the analysis device main body 102. As shown in FIG. 27, the analyzing device 100 is formed by bonding a base substrate 1 and a cover substrate 2 together.

  The base substrate 1 formed of a transparent synthetic resin is formed with an internal recess to be a holding chamber 3, a reagent chamber 4, a flow channel 5, a measurement chamber 6, and a flow channel 7 on the bonding surface 1 a with the cover substrate 2. Has been. The reagent chamber 4 carries an analysis reagent (not shown). Each cover surface of the internal recess is closed by a cover substrate 2 formed of a transparent synthetic resin to form a cavity having a gap of a predetermined size, transfer of the sample liquid by capillary force, and a predetermined amount of liquid Each function comes to work, such as holding the amount. Reference numeral 8b denotes an air opening hole formed on the cover substrate 2 corresponding to the position of the outlet 8a on the base substrate 1 side.

  The spotting portion 103 is formed by joining the protrusion 9 of the base substrate 1 and the protrusion 10 of the cover substrate 2, and the protrusion length L 1 of the protrusion 9 from the analysis device body 102 and the analysis device body 102. The protrusion length L2 of the protrusion 10 is formed to be the same. The tip of the spotting portion 103 and the holding chamber 3 are connected by a supply capillary channel 11 formed between the base substrate 1 and the cover substrate 2 as shown in FIGS. .

  When blood is analyzed as a sample solution, the posture of the analysis device 100 is vertical as shown in FIG. 30, and the spotting portion 103 is brought into contact with the blood reservoir 13 of the examinee's fingertip 12. The blood as the sample is sucked up to the holding chamber 3 by the capillary force of the supply capillary channel 11 and the holding chamber 3. FIG. 31 shows an enlarged view of the holding chamber 3, the reagent chamber 4, and the supply capillary channel 11 formed on the bonding surface 1 a of the base substrate 1.

  However, when the speed of the blood sucked up depends on the posture of the supply capillary channel 11 and the holding chamber 3, and it takes a short time to contact the spotting portion 103 with the blood reservoir 13, or the posture is inappropriate. However, there is a problem that it is not possible to sample only a fixed amount of blood necessary for performing an accurate analysis.

  It is an object of the present invention to provide an analytical device having a structure in which it is easy to visually confirm that a quantitative sample liquid has been sucked up in an analytical device that is brought into contact with a sample liquid reservoir and sucked up by capillary force. To do.

  In the analysis device according to claim 1 of the present invention, one end of the supply capillary channel is opened at the spotted portion formed in the analysis device body, and the supply capillary channel is inside the analysis device body. The sample solution connected to the spotted portion is connected to the microchannel structure formed in the capillary channel of the supply capillary channel and the capillary force of the holding chamber formed inside the analysis device body. An analytical device used for reading to access the solution in the measurement chamber by sucking and transferring the sample liquid in the holding chamber toward the measurement chamber formed inside the analysis device main body by centrifugal force, At the end of the holding chamber, a filling confirmation region of a gap that is smaller or larger than the gap that generates the capillary force of the holding chamber is provided. It is characterized in that form.

The analysis device according to claim 2 of the present invention is characterized in that, in claim 1, a confirmation window is formed in the analysis device main body corresponding to the filling confirmation region.
According to a third aspect of the present invention, there is provided the analytical device according to the first aspect, wherein the cover substrate and a base substrate in which an internal recess that constitutes the holding chamber is formed on a bonding surface of the cover substrate are bonded to each other. Each opening surface of the internal recess of the holding chamber is closed by a cover substrate to constitute the analysis device main body, and a gap smaller than a gap for generating the capillary force of the holding chamber provided at the terminal end of the holding chamber. Is formed between the cover substrate and the tip of a convex portion protruding from the base substrate side toward the cover substrate.

  According to a fourth aspect of the present invention, there is provided the analytical device according to the first aspect, wherein the cover substrate and a base substrate in which an internal recess constituting the holding chamber is formed on a bonding surface of the cover substrate are bonded to each other. Each opening surface of the internal recess of the holding chamber is closed with a cover substrate to constitute the analysis device main body, and a gap larger than a gap for generating the capillary force of the holding chamber provided at the terminal end of the holding chamber. Is formed between the cover substrate and the bottom of a recess that has entered the base substrate side toward the opposite side of the cover substrate.

  According to a fifth aspect of the present invention, the analytical device according to the fourth aspect is characterized in that a concave portion is formed in the cover substrate corresponding to the concave portion provided on the base substrate side.

  According to this configuration, when the filling confirmation region formed at the end portion of the holding chamber is in contact with the sample solution reservoir by contacting the spotting portion, the sample solution is sucked up to the filling confirmation region, When the filling confirmation region is formed in a gap smaller than the gap that generates the capillary force in the holding chamber, when the sample liquid is completely sucked up, the state in which the sample liquid is sucked into the small gap is visually observed. The shortage of the sample solution can be resolved by confirming with and ending the sampling. In addition, when the filling confirmation region is formed in a gap larger than the gap that generates the capillary force of the holding chamber, the sample liquid is sucked up and sandwiched around the large gap when the suction of the sample liquid is completed. The shortage of the sample solution can be resolved by visually confirming the state and ending the sampling.

Hereinafter, the analysis device of the present invention will be described based on each embodiment shown in FIGS.
(Embodiment 1)
1 to 6 show Embodiment 1 of the present invention.

In addition, the same code | symbol is attached | subjected and demonstrated to what performs the effect | action similar to FIGS.
The analysis device 100A is configured by bonding the base substrate 1 and the cover substrate 2 shown in FIG. Specifically, the base substrate 1 and the cover substrate 2 are formed of a synthetic resin such as a transparent acrylic resin.

  In the base substrate 1, internal recesses to be a holding chamber 3, a reagent chamber 4, a flow path 5, a measurement chamber 6, and a flow path 7 are formed on the bonding surface 1 a with the cover substrate 2. The reagent chamber 4 carries an analysis reagent (not shown). Each cover surface of the internal recess is closed by a cover substrate 2 formed of a transparent synthetic resin to form a cavity having a gap of a predetermined size, transfer of the sample liquid by capillary force, and a predetermined amount of liquid Each function comes to work, such as holding the amount. Reference numeral 8b denotes an air opening hole formed on the cover substrate 2 corresponding to the position of the outlet 8a on the base substrate 1 side.

  Further, the supply capillary channel 11, the holding chamber 3, and the walls of the channels 5 and 7 are subjected to hydrophilic treatment. Examples of the hydrophilic treatment method include a surface treatment method using an active gas such as plasma, corona, ozone, and fluorine, and a surface treatment using a surfactant or a hydrophilic polymer. Here, hydrophilicity means that the contact angle with water is less than 90 °.

  The specific size of the analysis device 100A is such that when the thickness of the base substrate 1 is 15 mm, the thickness of the cover substrate 2 is 1 mm, and the analysis device 100A is configured with approximately 80 mm square, the depth of the holding chamber 3 is 0. 0.02 mm to less than 0.3 mm. The depth of the holding chamber 3 when measuring and analyzing a liquid such as blood is preferably 0.1 mm. The depth of the reagent chamber 4 exceeds 0.3 mm and is formed to be deeper than the depth of the holding chamber 3 by ˜0.5 mm. By setting in this way, the blood injected into the holding chamber 3 does not proceed to the reagent chamber 4 only by the capillary force, and the sample solution is obtained by utilizing the centrifugal force obtained by rotating the analysis device 100A. This is for transportation.

  The supply capillary channel 11, the holding chamber 3, the channel 5, and the channel 7 are formed with a depth of 0.02 mm to less than 0.3 mm. If the sample liquid flows by capillary force, this dimension is used. It is not limited to. In addition, the depth of the reagent chamber 4 and the measurement chamber 6 is formed to be more than 0.3 mm and 0.5 mm. This depends on the amount of sample solution and the conditions for measuring the absorbance (optical path length, The measurement wavelength, the reaction concentration of the sample solution, the type of reagent, etc. can be adjusted. Then, the sample liquid transferred to the measurement chamber 6 is optically measured.

  The spotting portion 103 is formed by joining the projection 9 of the base substrate 1 and the projection 10 of the cover substrate 2. The space between the tip of the spotting portion 103 and the holding chamber 3 is shown in FIGS. 2 and 3. In this way, the supply capillary channel 11 is connected between the base substrate 1 and the cover substrate 2.

  In the case where the sample liquid is blood, all the supply capillary channels 11 and most of the gaps of the holding chamber 3 are formed to be less than 0.3 mm, for example, and the gap of the reagent chamber 4 exceeds 0.3 mm. It is formed to be 0.5 mm or less.

Only the following points differ from the comparative example shown in FIGS. 26 to 31.
As shown in FIGS. 3 and 4, a filling confirmation region 3 a having a gap (0.1 mm) smaller than the gap (0.3 mm) that generates the capillary force of the holding chamber 3 is formed at the terminal end of the holding chamber 3. The convex part 3b to be formed is different from the comparative example in that the convex part 3b to be formed is formed on the base substrate 1 in the first embodiment. Concave portions 3c and 3d are formed on both sides of the convex portion 3b.

  In addition, a confirmation window 20 is formed on the surface 1b of the base substrate 1 opposite to the bonding surface 1a corresponding to the filling confirmation region 3a, as shown in FIGS. For example, the periphery of the confirmation window 20 on the surface 1b is textured so that the translucency is lower than that of the confirmation window 20. Specifically, it is configured with a satin pattern on the surface.

  Since it comprised in this way, when implementing the analysis of the blood as a sample liquid, by making the attitude | position of the device 100A for analysis perpendicular | vertical and making the spotting part 103 contact the blood reservoir 13 of a patient's fingertip 12, By the capillary force of the supply capillary channel 11 and the holding chamber 3, blood 21 as a sample first flows along the wall surfaces 22a and 22b of the holding chamber 3 as shown in FIG. It is sucked up in a shape retreated from the side of 22a, 22b. In the middle of the suction, the sucked blood cannot be confirmed from the confirmation window 20.

  When the sucked blood reaches a fixed amount, the sucked blood reaches the filling confirmation region 3a as shown in FIG. 5B, and the convex portion 3b is formed there, as shown in FIG. 5A. Since the central portion 23 changes to a shape protruding toward the reagent chamber 4 and can be confirmed from the confirmation window 20 instead of the tip shape where the central portion 23 is retracted, it is clearly read that the sampling amount has reached a fixed amount. be able to.

In the case of the comparative example shown in FIG. 31, it is difficult to visually confirm from the surface 1b that the sucked blood is in a fixed state as shown in FIGS.
In the first embodiment, the convex portion 3b is provided on the base substrate 1 side and the gap is formed to be 0.1 mm. However, the convex portion 3b is provided on the cover substrate 2 side and the end portion of the holding chamber 3 is set to 0. A 1 mm gap can also be formed.

(Embodiment 2)
7 to 13 show a second embodiment of the present invention.
As shown in FIGS. 7 and 8, the analysis device 100 </ b> B configured by bonding the base substrate 1 and the cover substrate 2 has the tip of the spotting portion 103 formed by the inclined surface 14. The point different from the first embodiment is that one end of the supply capillary channel 11 is open on the surface 14.

  Since the tip of the spotting portion 103 is formed on the inclined surface 14, the spotting portion 103 formed by joining the projection 9 of the base substrate 1 and the projection 10 of the cover substrate 2 is the projection length of the projection 10. The length L2 is shorter than the protrusion length L1 of the protrusion 9. Moreover, as shown in FIG. 9, the angle θ of the inclined surface 14 is an acute angle. Specifically, when the sample solution is blood, it is preferably 30 ° to 45 °. FIG. 9 shows a state in which the opening 101 which is one end of the supply capillary channel 11 is opened at the inclined surface 14.

The width W1 of the protrusion 9 of the base substrate 1 near the opening 101 and the width W2 of the protrusion 10 of the cover substrate 2 near the opening 101 are formed to be the same.
The width W1 of the protrusion 9 of the base substrate 1 and the width W2 of the protrusion 10 of the cover substrate 2 are 3 to 5 mm, and the protruding length L1 of the spotting portion 103 from the analysis device body 102 is 8 mm.

  The spotting portion 103 is formed by joining the projection 9 of the base substrate 1 and the projection 10 of the cover substrate 2, and the configuration of the filling confirmation region 3 a at the end portion of the holding chamber 3 and the confirmation window 20 is the embodiment. 1 and the supply capillary channel formed between the base substrate 1 and the cover substrate 2 as shown in FIGS. 11 and 13 between the tip of the spotting portion 103 and the holding chamber 3. 11 is connected.

  With this configuration, when blood is analyzed as a sample solution, the posture is vertical and the tip of the spotted portion 103 is placed at the fingertip of the examinee as in the analysis device 100B indicated by the phantom line in FIG. 12, the opening 101 at one end of the supply capillary channel 11 opened at the inclined surface 14 does not come into contact with the blood reservoir 13, so that the holding chamber is supplied from the supply capillary channel 11. 3 is not sucking up blood.

  Therefore, when the analysis device 100B is tilted as shown by a solid line in FIG. 12 and the inclined surface 14 is moved along the fingertip 12, the opening 101 opened at the inclined surface 14 contacts the blood reservoir 13, Blood is sucked into the holding chamber 3 from the supply capillary channel 11.

  Since the tip shape of the spotting portion 101 is formed on the inclined surface 14 as described above, the length of the supply capillary channel 11 is longer than the distance shown in FIG. 13 compared to the comparative example shown in FIG. In addition to being shortened by L3, the angle of the supply capillary channel 11 and the holding chamber 3 at the time of suction is 30 ° to 45 °, which is the same as the angle θ of the inclined surface 14, and the comparative example shown in FIG. As compared with the case where the angle of the supply capillary channel 11 and the holding chamber 3 is vertical, the magnitude of gravity that affects the speed of the sucked blood can be reduced, and a fixed amount of blood is held in the holding chamber. 3, the time required for sampling can be shortened compared with the comparative example of FIG.

  Further, when the sucked blood reaches a fixed amount, the sucked blood reaches the filling confirmation region 3a, and the sucked blood can be confirmed from the confirmation window 20, and the blood held in the holding chamber 3 is removed by centrifugal force. Accurate analysis can be performed when transported towards the measurement chamber 6 and optically accessing and analyzing the solution in the measurement chamber 6.

(Embodiment 3)
14 and 15 show a third embodiment of the present invention.
In the case of the second embodiment, when the analysis device 100B is pressed too much against the fingertip 12 of the examinee as shown in FIG. 15 (b), the opening 101 opened at the inclined surface 14 is formed by the fingertip 12. It is conceivable that the blood sucking speed is reduced due to obstruction. On the other hand, the third embodiment is different from the second embodiment in that an obstruction prevention recess 15 communicating with the opening 101 is formed on the inclined surface 14 as shown in FIG. Specifically, the cover substrate 2 is the same as that of the first embodiment, but the blocking prevention recess 15 is formed in the base substrate 1.

  The structure of the filling confirmation region 3a at the end of the holding chamber 3 and the confirmation window 20 is the same as in the first embodiment, and the space between the tip of the spotting portion 103 and the holding chamber 3 is as shown in FIG. They are connected by a supply capillary channel 11 formed between the base substrate 1 and the cover substrate 2.

  Because of this configuration, even when the analysis device 100B is pressed too much against the fingertip 12 of the examinee, the occlusion prevention recess 15 prevents the fingertip 12 from contacting the opening 101 as shown in FIG. In this case, the blood suction speed does not decrease.

(Embodiment 4)
16 to 19 show a fourth embodiment of the present invention.
In Embodiment 2, since the inclined surface 14 is formed in the spotting portion 103, when the inclined surface 14 is brought into contact with the blood reservoir 13, the area wetted by blood becomes larger than that in the comparative example shown in FIG. The blood that cannot be sucked into the supply capillary channel 11 by the capillary force remains at the tip of the base substrate 1 and solidifies there, but in the fourth embodiment, the blood remaining at the tip of the base substrate 1 can be reduced. .

  In the second embodiment, the width W1 of the protrusion 9 of the base substrate 1 and the width W2 of the protrusion 10 of the cover substrate 2 are formed to be the same, but in this third embodiment, the inclined surface 14 of the spotting portion 103 is formed. The width W2 near the opening 101 of the protrusion 10 of the cover substrate 2 is formed narrower than the width W1 of the base end of the protrusion 9 of the base substrate 1. In FIG. 16, the tip of the protrusion 10 of the cover substrate 2 is located near the center of the protrusion 9 of the base substrate 1, and the one end of the supply capillary channel 11 is connected to both sides 10R, 10R of the protrusion 10 as shown in FIG. 10L and the tip 10T of the protrusion 10 are open.

  The configuration of the filling confirmation region 3a at the end of the holding chamber 3 and the confirmation window 20 are the same as those in the first embodiment, and the space between the tip of the spotting portion 103 and the holding chamber 3 is as shown in FIG. They are connected by a supply capillary channel 11 formed between the base substrate 1 and the cover substrate 2.

  19A, the blood 13a remaining on the protrusions 9 of the base substrate 1 starts to be sucked into the holding chamber 3 via the supply capillary channel 11 by the capillary force as shown in FIG. The base substrate 1 of the base substrate 1 is supplied from the supply capillary channel 11 formed between the tip of the protrusion 10 of the cover substrate 2 whose tip is narrower than the protrusion 9 of the substrate 1 and the protrusion 9 of the base substrate 1. Most of the blood 13a remaining on the inclined surface 14 of the projection 9 can be sucked up almost by capillary force as shown in FIG. 19 (b).

  In each of the above embodiments, the analytical device 100B can be tilted in the horizontal direction as the inclination of the spotted portion becomes sharper, which is effective in shortening the filling time. The effect of the inclination of the spotting part 103 when the sample liquid is blood is confirmed to be in the range of 30 to 45 °. However, if the sample solution has an effect on the filling time even at 45 ° or more, it is limited to this angle. It is not something.

(Embodiment 5)
20 to 23 show a fifth embodiment of the present invention.
In the first embodiment, a convex portion that forms a filling confirmation region 3a having a gap (0.1 mm) smaller than a gap (0.3 mm) that generates the capillary force of the holding chamber 3 at the end portion of the holding chamber 3 3b is formed, but in the fifth embodiment, a filling confirmation region 3a having a gap larger than a gap (0.3 mm) that generates the capillary force of the holding chamber 3 is formed at the end of the holding chamber 3. The difference is that the recess 3e to be formed is formed as shown in FIGS.

  Since it comprised in this way, the blood 21 as a sample initially flows along the wall surfaces 22a and 22b of the holding chamber 3 as shown in FIG. 21 (a), and the center part 23 has retracted from the wall surfaces 22a and 22b side. Sucked in shape. In the middle of the sucking, the blood sucked from the confirmation window 20 shown in FIG. 22 cannot be confirmed.

When the sucked blood becomes quantitative, the sucked blood reaches the filling confirmation region 3a as shown in FIG. 21B, and the sucked blood can be confirmed from the confirmation window 20.
The fifth embodiment is an embodiment in which the protrusion 3b in the first embodiment is used as the recess 3e to form the filling confirmation region 3a, but the convex in the second embodiment, the third embodiment, and the fourth embodiment is used. It can also be carried out by forming the filling confirmation region 3a with the portion 3b as the recess 3e.

(Embodiment 6)
FIG. 24 shows a sixth embodiment of the present invention.
In the fifth embodiment, a gap larger than the gap that generates the capillary force of the holding chamber 3 provided at the end portion of the holding chamber 3 enters the base substrate 1 toward the side opposite to the cover substrate 2. Although formed between the bottom of the recess 3e and the cover substrate 2, in the sixth embodiment, the recess 3f is formed in the cover substrate 2 corresponding to the recess 3e provided on the base substrate 1 side. This is different from FIG.

  When the surface of the cover substrate 2 is flat corresponding to the concave portion 3e provided on the base substrate 1 side as shown in FIG. 23 of the fifth embodiment, the blood of the sample solution is removed when the diameter of the concave portion 3e is small. In this case, the quantitative display when viewed from the confirmation window 20 may become unclear.

  On the other hand, by forming the recess 3f in the cover substrate 2 as in the sixth embodiment, the blood of the sample liquid flows along the surface of the cover substrate 2 even when the diameter of the recess 3e is small. Thus, it is possible to avoid the occurrence of the situation of entering the recess 3e, and the quantitative display when viewed from the confirmation window 20 can be made clear.

(Embodiment 7)
FIG. 25 shows a seventh embodiment of the present invention.
In the sixth embodiment shown in FIG. 24, the cover substrate 2 corresponds to the recess 3e provided on the base substrate 1 side so that blood of the sample solution does not enter the recess 3e along the cover substrate 2. Although the concave portion 3f is formed, in FIG. 25, a hydrophobic treatment portion 3g is provided on the surface of the cover substrate 2 corresponding to the concave portion 3e provided on the base substrate 1 side, which is seen in the sixth embodiment. The recess 3f is not provided. Specifically, when the base substrate 1 and the cover substrate 2 are formed of a synthetic resin such as a transparent acrylic resin, the hydrophobic treatment portion 3g is biodegradable on the surface of the cover substrate 2 having a flat surface. This is realized by a method of coating hydrophobic polyester by treating it with high-temperature and high-pressure water.

Even in this case, the quantitative display when viewed from the confirmation window 20 can be made clear as in the sixth embodiment.
In each of the above embodiments, the confirmation window 20 is provided on the base substrate 1 side. However, the confirmation window 20 may be provided on the cover substrate 2 side corresponding to the filling confirmation region 3a.

  In each of the above embodiments, the case of an analytical device used for reading optically accessing a solution in the measurement chamber 6 has been described as an example. However, an electrochemical sensor is provided in the measurement chamber 6 to provide the solution with the solution. The same applies to the analytical device used for reading to access.

  The analytical device of the present invention is useful for measuring components of biological fluids with an electrochemical sensor or an optical sensor.

FIG. 3 is an external perspective view of the analysis device according to (Embodiment 1) of the present invention. The enlarged perspective view of the principal part of the base substrate of the same embodiment Usage state explanatory diagram of the same embodiment External perspective view of analysis device of the embodiment Enlarged view of the vicinity of the usage confirmation window of the same embodiment Enlarged view of the usage status of the comparative example External appearance perspective view of analytical device of (Embodiment 2) of the present invention Exploded perspective view of the analysis device of the same embodiment The enlarged perspective view of the principal part of the embodiment The enlarged plan view of the principal part of the embodiment The enlarged perspective view of the principal part of the base substrate of the same embodiment Usage state explanatory diagram of the same embodiment 12 is an enlarged cross-sectional view of the main part of FIG. The enlarged perspective view of the principal part of (Embodiment 3) of this invention Enlarged sectional view of the embodiment in use and enlarged sectional view of the comparative example in use The expanded perspective view of the principal part of (Embodiment 4) of this invention The enlarged plan view of the principal part of the embodiment The enlarged perspective view of the principal part of the base substrate of the same embodiment Plan view of usage state of the embodiment The expanded perspective view of the principal part of the base substrate of (Embodiment 5) of this invention Explanatory drawing of the usage state of the same embodiment External perspective view of analysis device of the embodiment The expanded sectional view of the use condition of the embodiment The expanded sectional view of the use condition of (Embodiment 6) of this invention The expanded sectional view of the use condition of (Embodiment 7) of this invention External perspective view of comparative analysis device Exploded perspective view of the comparative example An enlarged perspective view of the main part of the comparative example An enlarged plan view of the main part of the comparative example An enlarged sectional view of the comparative example in use The enlarged perspective view of the principal part of the base substrate of the comparative example

Explanation of symbols

100A, 100B Analysis device 102 Analysis device main body 103 Spotted part 1 Base substrate 1a Bonding surface of base substrate 1 to cover substrate 2 1b Surface of base substrate 1 opposite to bonding surface 1a 2 Cover substrate 3 Holding chamber 3a Filling confirmation region 3b Convex part 3c, 3d Concave part 3e, 3f Concave part 3g Hydrophobic treatment part 4 Reagent chambers 5, 7 Flow path 6 Measurement chamber 8b Air opening hole 11 Supplying capillary flow path 20 Confirmation window 22a, 22b Wall surface of holding chamber 3

Claims (5)

One end of a supply capillary channel is opened at a spotted portion formed in the analysis device body, and the supply capillary channel is connected to a microchannel structure formed inside the analysis device body, The sample solution attached to the attachment portion is sucked up by the capillary force of the capillary channel for supply and the capillary force of the holding chamber formed inside the analyzing device body, and the sample solution in the holding chamber is sucked up by the analyzing device body An analytical device used for reading that is transferred by centrifugal force toward a measurement chamber formed inside the chamber and that accesses the solution in the measurement chamber,
An analytical device in which a filling confirmation region of a gap smaller or larger than a gap in the holding chamber that generates the capillary force is formed at an end portion of the holding chamber. The analysis device according to claim 1, wherein a confirmation window is formed in the analysis device main body corresponding to the filling confirmation region. A cover substrate and a base substrate on which an internal concave portion constituting the holding chamber is formed are bonded to a bonding surface of the cover substrate, and each opening surface of the internal concave portion of the holding chamber is closed by the cover substrate. Configure the device body for analysis,
The gap that is smaller than the gap that generates the capillary force of the holding chamber provided at the terminal end of the holding chamber is a gap between the tip of the convex portion that protrudes from the base substrate side toward the cover substrate and the cover substrate. The analytical device according to claim 1, formed in between. A cover substrate and a base substrate on which an internal concave portion constituting the holding chamber is formed are bonded to a bonding surface of the cover substrate, and each opening surface of the internal concave portion of the holding chamber is closed by the cover substrate. Configure the device body for analysis,
The gap that is larger than the gap that generates the capillary force of the holding chamber provided at the end of the holding chamber has a bottom of a recess that enters the side of the base substrate toward the opposite side of the cover substrate and the cover. The analytical device according to claim 1, wherein the analytical device is formed between the substrate and the substrate. The analytical device according to claim 4, wherein a concave portion is also formed in the cover substrate corresponding to the concave portion provided on the base substrate side.

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