JP5269160B2 - Bioactive liquid composition and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a physiologically active liquid composition having an excellent physiological activity such as an immunostimulating effect and an anti-cancer effect. <P>SOLUTION: The physiologically active liquid composition contains: (A) an extracted component of Taxus chinensis; (B) an extracted component of Inonotus obliquus; (C) an extracted component of Uncaria tomentosa; and (D) an extracted component of mycelia of Cordyceps sinensis. <P>COPYRIGHT: (C)2013,JPO&amp;INPIT

Description

  The present invention relates to a bioactive liquid composition having immunostimulatory activity, anticancer effect and the like, and a method for producing the same.

Japan's medical expenses were about 20 trillion yen in 1989 (6.1% of national income), but about 20 trillion yen in 20 years later, about 34 trillion yen (about 9% of national income). .1%), and it is still increasing. A particularly serious problem is that the proportion of elderly people aged 65 and over is currently increasing from 32.5% to 22.5% after 30 years. Furthermore, the medical expenses paid by the elderly currently account for about 54% of the total medical expenses, but it is predicted that the proportion of the medical expenses in the national income will increase further as the population ages. There is a need for a stable society that avoids this problem and is healthy and long-lived.
Under such circumstances, various health foods and supplements containing extracts from plants and fungi, which are expected to have medicinal effects such as immunostimulatory effect and anticancer effect, have been reported.

For example, Patent Literatures 1 to 4 disclose health teas and health foods containing components extracted from hot cedar trunks with warm water or sake.
In Patent Documents 5 to 7, other ingredients (Western ginseng, ganoderma, cinnamon, etc.) are added to the powder obtained by crushing the stem of the main ingredient, red bean cedar under vacuum pressure, and boiled in water. A health food is disclosed in which a mixture obtained by adding a concentrated liquid is powdered by a dry-free manufacturing method.

  Moreover, as an example of a functional food to which an extract of birch moth is added, Patent Document 8 describes a functional food to which an enzyme-treated extract is added, and Patent Document 9 describes a cell wall of birch moth after destruction using a supercritical fluid. A functional food to which an extract obtained by extraction is added is disclosed.

  In addition, as an example of an extract of Cordyceps sinensis, Patent Document 10 discloses that as an extraction technique for an active ingredient of Cordyceps sinensis after ultrasonically pulverizing to 10 μm or less, enzymatic treatment with protease or cellulase and extraction with hot water at 120 ° C. An extraction method is disclosed. Patent Document 11 discloses that an extract obtained by extracting mycelium of Cordyceps sinensis with an aqueous solution at 40 to 50 ° C. is used for health foods and supplements.

JP-A-10-120582 JP-A-10-136934 Japanese Patent Laid-Open No. 10-136935 JP-A-10-136936 JP 2000-236835 A JP 2000-236836 A JP 2000-236838 A JP 2004-161748 A JP 2006-129714 A JP 2002-262820 A JP 2011-50338 A

Among the above medicinal plants and fungi, red soybean cedar containing abundant anticancer activity such as taxol is attracting particular attention because it can be expected to have an anticancer effect, but most of them are in high mountain areas such as Yunnan Province in China. It is inhabited, and logging and export are strictly restricted from the viewpoint of resource conservation, and it is difficult to obtain. For this reason, it is desired to collect extracts not only from the trunk of red bean cedar, which has been used as a raw material, but also from branches and leaves that have been discarded.
However, the extraction methods of Patent Documents 1 to 7 enable extraction from the trunk of red bean cedar that contains a large amount of medicinal ingredients, but the amount that can be extracted from the branches and leaves that have been conventionally discarded is never large. However, the current situation is exploring more efficient extraction methods.

  In addition, in the birch bamboo, the conventional extraction methods disclosed in Patent Documents 8 and 9 are expensive, using enzymes and supercritical fluids, and have disadvantages such as deposits and suspended matter remaining in the product. .

The technology for extracting active ingredients from Cordyceps sinensis described in Patent Document 10 requires an expensive ultrasonic pulverization apparatus for pre-treatment, and also performs an enzyme treatment with an expensive protease or cellulase enzyme, which is economical. Challenges remain. Moreover, since hot water of 120 ° C. is used for extraction, energy costs are increased, and at the same time, the activity of beneficial components may be lost.
In addition, the extraction technique described in Patent Document 11 is a method that can extract an active ingredient from Cordyceps at low cost, but there is room for improvement in terms of extraction efficiency.

Inflammation such as cancer, influenza, and rheumatism is caused by intricately intertwining various functions of the human body. Each of the above-described extractive components has an effect alone, but there are many unclear parts about the effectiveness of the effective component, and it is a fact that the details when combining a plurality of components are still unknown. For example, although details are unknown, even if a medicinal plant or fungus containing an active ingredient that exerts the above-mentioned analgesic action or anti-inflammatory action is taken as it is, for example, in combination with a plurality of substances, the effect of just using a plurality of them is obtained. It may not be possible.
In particular, red soybean cedar, which contains abundant anticancer activity, is expected to be used in combination with other medicinal plants and fungi that have enhanced immunity and antioxidative properties in order to enhance its efficacy and enhance synergistic effects. However, there is still no satisfaction.

  Under such circumstances, an object of the present invention is to provide a physiologically active liquid composition having physiological activities such as an excellent immunostimulatory effect and anticancer effect, and an efficient production method thereof.

In addition to red bean cedar, the present inventors focused on the interaction between the medicinal components of other medicinal plants and fungi, and as a result of creative combinations of these, the synergistic effect of the effective action of each raw material is demonstrated. I found a possible combination.
Furthermore, the present inventors have completed the present invention by finding a method for extracting an active ingredient while suppressing a decrease in activity from each raw material.

That is, the present invention relates to the following inventions.
<1> After crushing the branches and leaves of red bean cedar, primary extraction is performed with butanol to obtain a primary extract and an extraction residue, and the obtained extraction residue is subjected to secondary extraction with warm water at 70 to 95 ° C. extractives of Benimamesugi for (a), extract component of Kabanoanatake (B), Cat's claw extract component (C), and Cordyceps bioactive liquid composition containing the mycelium extract component of fungus (D).
< 2 > The bioactive liquid composition according to <1> , wherein the component (D) is an extractive component of mycelium of Cordyceps sinensis.
< 3 > After crushing the branches and leaves of red cedar cedar, it is first extracted with butanol to obtain a primary extract and an extraction residue, and the obtained extraction residue is secondarily extracted with hot water at 70 to 95 ° C. Obtaining an extract containing the extracted component (A) of red bean cedar;
After crushing birch bamboo, it is first extracted with ethanol to obtain a primary extract and an extraction residue. The obtained extraction residue is secondarily extracted with hot water at 70 to 95 ° C., and the extract component (B) of birch is taken. Obtaining an extract comprising:
After pulverizing the cat's claw, extraction with warm water at 70 to 95 ° C to obtain an extract containing the cat's claw extract component (C);
After crushing mycelia of Cordyceps, extracting with warm water at 70 to 95 ° C. to obtain an extract containing the extract component (D) of Cordyceps mycelium;
Mixing an extract containing the extraction components (A) to (D);
A method for producing a physiologically active liquid composition comprising:
< 4 > In the step of obtaining the extracted component (A) of red bean cedar, the dried product obtained by drying the primary extract is used as an extraction residue for primary extraction, warm water of 70 to 95 ° C. used for secondary extraction, And the manufacturing method of the bioactive liquid composition as described in said < 3 > including the process added to at least 1 among the extract containing the extract component (A) of the red bean cedar obtained by secondary extraction.
< 5 > In the step of obtaining the extract component (B) of birch salmon, a dry product obtained by drying the primary extract is used as an extraction residue for primary extraction, hot water at 70 to 95 ° C. used for secondary extraction, and The method for producing a physiologically active liquid composition according to the above < 3 > or < 4 >, comprising a step of adding to at least one of the extract containing the extract component (B) of birch salmon obtained by secondary extraction.
< 6 > The method for producing a physiologically active liquid composition according to any one of < 3 > to < 5 >, wherein after the step of mixing the extraction components (A) to (D), various bacteria are removed by filtering.

  The bioactive liquid composition of the present invention has physiological activities such as an immunostimulatory effect and an anticancer effect. According to the production method of the present invention, an extract can be efficiently obtained from each raw material without reducing the activity, and thus the physiologically active liquid composition can be produced stably.

It is the result 24 hours afterward about a test group and a control group in evaluation of a bioactivity effect (anticancer action). It is the result 48 hours afterward about a test group and a control group in evaluation of a bioactivity effect (anticancer action).

  The present invention relates to a physiologically active liquid composition containing an extract component (A) of red cedar cedar, an extract component (B) of birch salmon, an extract component (C) of cat's claw, and an extract component (D) of cordyceps mycelium.

The physiologically active liquid composition of the present invention has taxol, alkaloid, SOD-like component, β-glucan and the like contained in each raw material, and has physiological activities including immunostimulatory activity and anticancer activity.
Therefore, when the physiologically active liquid composition of the present invention is orally ingested, it can exhibit immunostimulatory activity and anticancer activity, and also has antirheumatic activity, antioxidant activity, analgesic activity, calming effect, anti-inflammatory effect. It can exert its action, immunity enhancing action, blood circulation improving action, antitumor action, hypoglycemic action and the like.

  Hereinafter, the extraction components (A) to (D) will be described in detail.

The extract component (A) is an extract obtained by contacting red cedar with, for example, an extract medium composed of water, methanol, ethanol, butanol, 1,3-butylene glycol, propylene glycol, or a mixture thereof, The concentrate and the dried product are mentioned.
As raw materials, trunks, leaves, and branches of red cedar can be used, and a mixture thereof may be used.
In addition, although mentioned later in detail, according to the manufacturing method of this invention which performs primary extraction with butanol, an extraction component (A) can be efficiently extracted not only from the stem part of a red cedar cedar but also from the leaf part and branch part of a red cedar Can be obtained.

Extraction component (B) is an extract obtained by bringing birch salmon into contact with an extraction medium composed of, for example, water, methanol, ethanol, butanol, 1,3-butylene glycol, propylene glycol or a mixture thereof, A concentrate and a dried product are mentioned.
The birch is a cold-tolerant mushroom that parasitizes birch and other birch species such as birch and birch and grows using the sap of these trees as nutrients. Its components include physiological activities such as antitumor activity and hypoglycemic activity. Have
As the raw material, fruit bodies, mycelium and mycelium of birch oyster mushroom can be used, and a mixture thereof may be used. The preferred extraction target is mycorrhiza, and it is preferable to pulverize the mycelia to increase the extraction efficiency of the physiologically active component.

Extraction component (C) is an extract extracted by bringing Cat's Claw into contact with an extraction medium comprising, for example, water, methanol, ethanol, butanol, 1,3-butylene glycol, propylene glycol or a mixture thereof, The concentrate and the dried product are mentioned.
Cats Claw (Uncaria Tomentosa) is a vine plant that grows naturally in the high altitudes of 400 to 800m above sea level in the Peruvian Amazon. Contains various medicinal ingredients such as acids, plant sterols, and tannins.
Cats claw roots and bark can be mainly used as a raw material, and a mixture thereof may be used. In order to increase the extraction efficiency of the physiologically active ingredient, it is preferable to use the roots and bark after pulverization.

The extract component (D) is an extract component of cordyceps mycelium. Extraction component (D) is an extract obtained by bringing the mycelium of Cordyceps sinensis into contact with an extraction medium composed of, for example, water, methanol, ethanol, butanol, 1,3-butylene glycol, propylene glycol, or a mixture thereof. Liquid, its concentrate, and dried product.
Cordyceps sinensis, Cordyceps sobolifera, Cordyceps militaris, Cordyceps militaris, and Mikakida (Coldyceps militaris), as well as Cordyceps sinensis isolated from naturally occurring Cordyceps spp. Cordyceps nutans) etc. can be used. It is preferable to use those purified by solid and liquid culture. Preferably, mycorrhizal fungus mycelium is obtained by surface-cultivating fruit bodies of Cordyceps sinensis on a phenicol agar medium and then further culturing in a potato liquid medium. By using the mycelium thus purified, impurities such as precipitates and suspended matters are generated in the bioactive liquid composition of the present invention containing the extract component (D) and the extract component (D). It is hard to happen.

Among these cordyceps, Chinese natural cordyceps sinensis is preferable because it has the highest physiological activities such as immunostimulatory activity and antitumor activity.
Cordyceps sinensis (especially purified) is used to inoculate unused organic matter such as food waste such as shochu, soy sauce cake, vegetable scraps, etc. By culturing for about 10 to 25 days in a dark room of 80% or more, beneficial components such as amino acids, ergosterol, β-glucan, vitamin E, SOD (superoxide dismutase) substances are generated, and especially immunostimulatory activity And is preferably used because of its high antioxidant property.
The physiologically active liquid composition of the present invention can be produced by mixing the extraction components (A) to (D). The mixing ratio is selected in consideration of the target efficacy, the age, sex, and constitution of the subject. In addition, the weight of each extraction component is the weight of the dried product obtained by sufficiently distilling off the solvent.

The physiologically active liquid composition of the present invention may contain other components in addition to the extracted components (A) to (D).
As other ingredients, various additives used in foods, specifically, coloring agents, preservatives, thickening stabilizers, antioxidant bleaches, antibacterial and antifungal agents, acidulants, seasonings, emulsifiers , Reinforcing agents, production agents, fragrances and the like. Moreover, you may add another medicinal plant and fungi in the range which does not impair the effect of this invention.

Hereinafter, a method particularly suitable for producing the physiologically active liquid composition of the present invention (hereinafter referred to as “the production method of the present invention”) will be described.
In the production method of the present invention, the branches and leaves of red bean cedar are pulverized, and then subjected to primary extraction with butanol to obtain a primary extract and an extraction residue, and the resulting extraction residue is subjected to secondary heating with warm water at 70 to 95 ° C. Extracting to obtain an extract containing the extract component (A) of red cedar,
After crushing birch bamboo, it is first extracted with ethanol to obtain a primary extract and an extraction residue. The obtained extraction residue is secondarily extracted with hot water at 70 to 95 ° C., and the extract component (B) of birch is taken. Obtaining an extract comprising:
After pulverizing the cat's claw, extraction with warm water at 70 to 95 ° C to obtain an extract containing the cat's claw extract component (C);
After crushing mycelia of Cordyceps, extracting with warm water at 70 to 95 ° C. to obtain an extract containing the extract component (D) of Cordyceps mycelium;
Mixing an extract containing the extraction components (A) to (D);
It is characterized by including.

One of the features of the production method of the present invention is that primary extraction is performed using butanol as an extraction medium in the method for extracting red soybean cedar.
Butanol penetrates into the structure of red cedar cedar and has a softening action. When primary extraction is performed by immersing red cedar in butanol, it contains a primary component (A) extracted from red cedar that dissolves in butanol. An extract and an extraction residue are obtained. Since this extraction residue is softened by butanol, the extracted component (A) of red cedar can be easily extracted by dipping in warm water at 70 to 95 ° C. and performing secondary extraction.
Here, butanol can soften not only the stems of red bean cedar but also the branches and leaves of red bean cedar, which are difficult to extract by conventional extraction methods using water and ethanol, so Even from the branches and leaves, the extracted component (A) can be obtained efficiently. In addition, in order to extract more, secondary extraction with respect to an extraction residue may be performed in multiple times.

Moreover, in the process of obtaining the extract component (A) of red bean cedar, the dried product obtained by drying the primary extract is used as an extraction residue of primary extraction, hot water of 70 to 95 ° C. used for secondary extraction, and It is preferable to include a step of adding to at least one of the extract containing the red cedar extract component (A) obtained by the secondary extraction.
The primary extract of red cedar cedar contains an extract component (A) of red cedar dissolved in butanol. Therefore, since the dried product obtained by distilling off the butanol of the primary extract contains a large amount of the extraction component (A), this dried product is used for the extraction residue of the primary extraction and the secondary extraction. The concentration of the extractable component (A) can be increased by adding to at least one of the extract containing the hot water of ˜95 ° C. and the extract component (A) of red bean cedar obtained by secondary extraction.

  The obtained extracted component (A) is preferably filtered to remove precipitates and impurities. The dried product of the primary extract contains components that dissolve in butanol but cannot be dissolved in water. Such components can also be removed by filtering.

  Furthermore, birch salmon is difficult to extract with warm water alone, so first extract it with ethanol to obtain a primary extract and an extraction residue, and then extract the resulting extraction residue with hot water at 70-95 ° C. It is more efficient to obtain an extract containing the extract component (B) of birch moth. In addition, in order to extract more, secondary extraction with respect to an extraction residue may be performed in multiple times.

In addition, in the step of obtaining the extract component (B) of birch salmon, the dried product obtained by drying the primary extract is extracted residue of primary extraction, hot water of 70 to 95 ° C. used for secondary extraction, and 2 It is preferable to include a step of adding to at least one of the extract solutions containing the extract component (B) of birch salmon obtained by the next extraction.
The primary extract of birch moth contains the extract component (B) of birch moth that dissolves in ethanol. Therefore, since the dried product obtained by distilling off ethanol from the primary extract contains a large amount of the extraction component (B), this dried product is used for the extraction residue of the primary extraction and the secondary extraction. The concentration of the extract component (B) can be increased by adding it to at least one of the extract containing the extract component (B) of hot water of ˜95 ° C. and birch salmon obtained by secondary extraction.
In addition, it is preferable to remove a deposit and an impurity from the obtained extraction component (B) by filtering.

Moreover, since the extract component (C) of cat's claw and the extract component (D) of cordyceps mycelium have high solubility in water, it can be sufficiently extracted by using hot water of 70 to 95 ° C. it can.
In addition, it is preferable to remove precipitates and impurities from the obtained extracted component (C) and extracted component (D) by filtering.

The physiologically active liquid composition of the present invention can be obtained by mixing the extracted components (A) to (D) and other components added as necessary.
The mixing order is arbitrary as long as there is no particular problem such as reaction or precipitation, and any two or more components among the components of the bioactive liquid composition are blended in advance, and then the remaining components May be mixed or all at once.

In the production method of the present invention, it is preferable to remove bacteria by filtering after the step of mixing the extracted components (A) to (D) and other components as necessary.
Examples of the filter include a filtration filter having a pore size of about 0.2 μm that can also remove E. coli. By performing filtering in this way, it is possible to suppress the propagation of germs and to prevent the occurrence of suspended matter and sediment of the product.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further in detail, this invention is not limited to a following example, unless the summary is changed.

  As raw materials, red bean cedar was from Yunnan, China, birch was taken from Russia, and cat's claw was from Peru.

Cordyceps mycelium was obtained as follows.
In a clean bench, fruit bodies of Cordyceps sinensis were cut, inoculated into a phenicol plate medium sterilized by autoclaving at 121 ° C. for 20 minutes, and cultured in an incubator set at 20 ° C. to 30 ° C. for 1 week. Next, the mycelium of Cordyceps sinensis cultured on a flat plate medium was taken out in a clean bench, inoculated into a potato liquid medium sterilized by autoclaving at 121 ° C. for 20 minutes, and in an incubator set at 20 ° C. to 30 ° C. for 1 week. Cultured.
Next, unused organic substances such as used rice, okara and shochu were packed in a heat-resistant container, sterilized with a retort sterilizer, etc., and then cooled to room temperature in an aseptic room. The mycelium cultured in liquid culture is taken out with a syringe, inoculated into unused organic matter that has been cooled to room temperature after sterilization, and placed in a dark fermentation room at 20 ° C to 28 ° C and a humidity of 80% or more for 10 days to 25 days. The mycelium of Cordyceps as a raw material was obtained by culturing for about a day.

The physiologically active liquid composition was produced as follows.
(1) A predetermined amount of red cedar branches and leaves were first extracted with butanol at room temperature after pulverization to obtain a primary extract and an extraction residue. Then, the dried product obtained by distilling out butanol from this primary extract is added to the extraction residue of the primary extraction, and then the extraction residue is heated to 70 ° C. to 95 ° C. with high extraction efficiency. The aqueous solution containing the red bean cedar extract (A) was obtained by removing the precipitate and impurities from the secondary extract by filtering a plurality of times.
The weight of the dried product obtained by sufficiently drying 1 L of the aqueous solution containing the red bean cedar extract (A) was 0.35 g.
(2) The birch was first extracted with ethanol at room temperature after pulverization to obtain a primary extract and an extraction residue. Thereafter, the dried product obtained by distilling off ethanol from the primary extract is added to the extraction residue of the primary extraction, and then the extraction residue is heated to 70 ° C. to 95 ° C. with high extraction efficiency. A secondary extract was obtained by performing extraction several times. The secondary extract was filtered to remove precipitates and impurities, thereby obtaining an aqueous solution containing the extract (B) of birch moss.
The weight of the dried product obtained by sufficiently drying 1 L of the aqueous solution containing the resulting birch salmon extract (B) was 3.85 g.
(3) After crushing the cat's claw, extraction was performed several times with warm water at 70 ° C to 95 ° C to obtain an aqueous solution containing an extract (C) of cat's claw. The precipitates and impurities were removed by filtering.
The weight of the dried product obtained by sufficiently drying 1 L of the aqueous solution containing the obtained catscrow extract (C) was 1.4 g.
(4) After pulverizing the mycelium of Cordyceps sinensis, extraction was performed several times with warm water at 70 ° C. to 95 ° C. to obtain an aqueous solution containing an extract (D) of Cordyceps mycorium. The precipitates and impurities were removed by filtering.
The weight of the dried product obtained by sufficiently drying 1 L of the aqueous solution containing the extract (D) of the cordyceps mycelium obtained was 1.4 g.
(5) Next, 5 wt% of the aqueous solution containing the extract (A), 55 wt% of the aqueous solution containing the extract (B), 20 wt% of the aqueous solution containing the extract (C), and the extract (D) The aqueous solution containing 20% by weight was mixed until it was sufficiently uniform at room temperature, and then passed through a filtration filter having a pore size of 0.2 μm that could finally remove E. coli, thereby obtaining the physiologically active liquid composition of the example. .

"Evaluation of bioactive effect (anticancer effect)"
For anticancer activity, colon cancer cells DLD-1 were ingested into a petri dish and the bioactivity effect was verified. The verification was divided into a control group to which nothing was added and a test group to which a bioactive composition was added, followed by follow-up for 24 hours and 48 hours, and the survival rate of cancer cells was measured with the WST-8 kit (Kishida Chemical Co., Ltd.) Measured with Table 1 shows the survival rate (compared to the control group) of cancer cells after 24 hours, 48 hours, and 72 hours.
Moreover, about the test group after 24 hours and 48 hours, and the control group, it shows in FIG. 1, FIG. 2 which shows the result measured with the phase-contrast fluorescence microscope (Leica fluorescence microscope, model number leica DFC300FX), respectively.

  1 and 2, it can be seen that cancer cells are alive in the control group, but clearly decreased in the test group. From this, it was confirmed that the present invention has a high cancer suppressing effect.

(Evaluation of immune activation effect and immune abnormality suppression effect)
About the bioactive liquid composition of an Example, the immunostimulation effect and the immune abnormality suppression effect were evaluated. Tests were performed under the following conditions, and blood of skid mice was collected and verified by increasing and decreasing the ratio and number of immune cells.

(1) Mouse used Skid mouse (SCID, B17 / Icr scid / scid male)
80 animals (5 animals x 4 x 4)
Target mouse (WT, B17 / Icr + / + male)

(2) Test method Test group 1: For 1 mouse, test group administered per day at 0.025cc 2: For one mouse, test group administered per day at 0.050cc 3: Test group per day administered 0.100cc per mouse : 0.150 cc daily for 1 mouse

・ N = 5 and blood was collected on the 1st, 7th and 14th

(3) Evaluation The ratio of immune cells in CD11b cells and CD49b cells in the blood was analyzed by Becton Dickinson's FACSVantage SE. Company). Table 2 shows the results of CD11b cells, Table 3 shows the results of CD49b cells, and Table 4 shows the results of TNF-α values.

  As shown in the table below, the results of the verification confirmed that the ratio of CD11b cells and CD49b cells in the blood increased, and that immunity was activated. On the other hand, both SCID and WT in the control group showed similar high values, but TNF-α level decreased significantly regardless of the concentration due to treatment, and decreased to below the detection sensitivity on the 7th day, and increased inflammatory factors We were able to verify that we were able to control normally.

  From the above verification results, it was confirmed that the physiologically active liquid composition of the present invention has an immunostimulatory effect, a cancer suppressive effect, and an inhibitory effect on inflammatory factors such as rheumatism.

  According to the present invention, a physiologically active liquid composition having physiological activities such as an excellent immunostimulatory effect and anticancer effect is provided.

Claims (6)

After crushing the branches and leaves of red bean cedar, it is first extracted with butanol to obtain a primary extract and an extraction residue, and the obtained extraction residue is secondarily extracted with hot water at 70 to 95 ° C. A physiologically active liquid composition comprising: an extract component (A) of the above, an extract component (B) of birch bamboo, an extract component (C) of cat's claw, and an extract component (D) of cordyceps mycelium. The bioactive liquid composition according to claim 1, wherein component (D) is an extractive component of mycelium of Cordyceps sinensis. After crushing the branches and leaves of red cedar, it is first extracted with butanol to obtain a primary extract and an extraction residue. The obtained extraction residue is secondarily extracted with hot water at 70 to 95 ° C. Obtaining an extract containing the extract component (A);
After crushing birch bamboo, it is first extracted with ethanol to obtain a primary extract and an extraction residue. The obtained extraction residue is secondarily extracted with hot water at 70 to 95 ° C., and the extract component (B) of birch is taken. Obtaining an extract comprising:
After pulverizing the cat's claw, extraction with warm water at 70 to 95 ° C to obtain an extract containing the cat's claw extract component (C);
After crushing mycelia of Cordyceps, extracting with warm water at 70 to 95 ° C. to obtain an extract containing the extract component (D) of Cordyceps mycelium;
Mixing an extract containing the extraction components (A) to (D);
A method for producing a physiologically active liquid composition comprising: In the step of obtaining the red bean cedar extract component (A), the dried product obtained by drying the primary extract is used as the extraction residue of the primary extraction, the hot water of 70 to 95 ° C. used for the secondary extraction, and the secondary The manufacturing method of the bioactive liquid composition of Claim 3 including the process added to at least 1 among the extract containing the extract component (A) of the red bean cedar obtained by extraction. In the step of obtaining the extract component (B) of birch, the dried product obtained by drying the primary extract is extracted from the primary extraction, the hot water at 70 to 95 ° C. used for the secondary extraction, and the secondary extraction. The manufacturing method of the bioactive liquid composition of Claim 3 or 4 including the process added to at least 1 among the extract containing the extract component (B) of the birch extract obtained by this. The method for producing a physiologically active liquid composition according to any one of claims 3 to 5 , wherein the bacteria are removed by filtering after the step of mixing the extraction components (A) to (D).