JP4350813B2 - Automatic compound synthesizer - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to automated synthesis equipment of compounds for synthesizing the compound of any mixing ratio for each reaction tube is arranged in the reaction block.
[0002]
[Prior art]
Recently, a method called combinatorial chemistry has been adopted when a novel compound is produced by combining several substances.
In this method, several kinds of reagents prepared in advance are mixed at an arbitrary mixing ratio for each test tube to synthesize a large number of samples having different mixing ratios, and select a compound having an optimal mixing ratio from them. Is.
Specifically, first, a reagent injection operation is performed in which a reagent is injected at an arbitrary mixing ratio for each of a large number of reaction tubes arranged in a rack, and then the reagent injected into the reaction tube is agitated and controlled in temperature to be predetermined. The reaction operation is performed to advance the chemical reaction.
When the synthesized compound is divided into multiple liquid layers after the reaction is completed, it is necessary to perform a liquid separation operation for inhaling the compound from a predetermined liquid layer portion and to filter the compound after the reaction is completed. Requires a filtration operation and various operations such as a column purification operation in order to analyze the compound after the reaction.
[0003]
In this case, since a large number of samples must be synthesized and the above-mentioned processes must be performed for each of them, recently, each process using a reagent injection apparatus, a reaction apparatus, a liquid separation apparatus, a filtration apparatus, a column purification apparatus, etc. Is done automatically.
According to this, first, a reagent is injected at a predetermined mixing rate into each reaction tube by the reagent injection device. Next, in the reaction apparatus, conditions such as a temperature condition, a time condition, and a stirring condition for performing a chemical reaction are programmed in advance, so that each reaction tube into which a reagent is injected at a predetermined mixing ratio is set in the reaction apparatus. Then, the reaction proceeds according to the program.
When the compound for which the reaction has been completed is divided into a plurality of liquid layers, the desired compound can be taken out from the predetermined liquid layer portion by applying it to a liquid separator, and if necessary, the reaction can be performed. After the compound in the tube is filtered through a filtration device, the analysis can be pretreated by a column purification device.
[0004]
[Problems to be solved by the invention]
However, when such an automatic machine is used, each operation is automated, but only one operation can be performed per unit. Therefore, an automatic machine must be installed for each operation. There was a problem that the bulky installation space was wasted.
Also, when trying to perform each of these operations on a single unit, simply gathering the devices together does not reduce the manufacturing cost so much, and the size of one unit increases, saving installation space. It is never done.
[0005]
Therefore, the present invention is not only capable of automatically performing various operations necessary for synthesizing a compound, but also extremely technically reducing the manufacturing cost by making it extremely compact and without wasting installation space. It is said.
[0006]
[Means for Solving the Problems]
In order to solve this problem, the present invention provides a chemical injection needle that is moved and controlled so as to be positioned at the position of each test tube arranged in the reagent rack and the position of each reaction tube arranged in the reaction block. In an automatic compound synthesizer that inhales a reagent stored in a test tube and injects a required amount into each reaction tube, and synthesizes a compound having an arbitrary mixing ratio for each reaction tube, the reaction block includes A stirring means for rotating a magnetic stirrer formed in a rotating magnetic field rotating in a circumferential direction of the reaction tube and a temperature control means for heating or cooling each reaction tube; The replacement rack mounting unit is detachably mounted on a mounting unit, and the replacement rack mounting unit replaces the reagent rack with a sample rack in which test tubes for storing the required amount of the reaction-completed compounds are arranged, and the reaction-completed compound. A filtration rack with a filter cartridge with a filter for injecting and filtering each required amount or a column purification rack with an extraction tube for column purification of the compound after the reaction has been formed. And
[0007]
According to the present invention, a reagent rack, a sample rack, a filtration rack, and a column purification rack are mounted on the replacement rack mounting portion in a replaceable manner.
Therefore, when performing a reagent injection operation, if a reagent rack is attached, the reagent stored in each test tube of the reagent rack is inhaled by the chemical solution injection needle and injected into each reaction tube arranged in the reaction block.
Next, the reaction block includes stirring means for forming a rotating magnetic field that rotates a magnet submerged in each reaction tube, and temperature control means for heating or cooling each reaction tube. A reaction operation is performed to stir the injected reagent and control the temperature to advance a predetermined chemical reaction.
[0008]
When performing a liquid separation operation, the sample rack is mounted on the replacement rack mounting portion, the compound is sucked from the predetermined liquid phase of the reaction tube with the chemical injection needle, and is discharged to the test tube of the sample rack. .
In addition, when performing a filtration operation, a filtration rack is attached to the replacement rack attachment part, and a chemical solution injection needle is used from a reaction tube arranged in the reaction block or a test tube arranged in the sample rack placed on the reaction block. The inhaled compound may be discharged into the filtration cartridge of the filtration rack.
Furthermore, when performing the column purification operation, the column purification rack may be mounted on the replacement rack mounting portion, and the compound sucked by the chemical solution injection needle may be discharged into the extraction tube of the column purification rack.
In this way, each operation can be performed automatically with a single device simply by exchanging the rack mounted on the replacement rack mounting part, so that the device itself can be made extremely compact and the manufacturing cost can be reduced. Is done.
[0009]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be specifically described below with reference to the drawings.
1 is a front view showing an automatic compound synthesizing apparatus according to the present invention, FIG. 2 is a cross-sectional view taken along the line II of FIG. 1, FIG. 3 is an enlarged view showing the internal structure of the reaction block, and FIG. FIG. 5 is a front view showing a state in which a TLC substrate support rack is attached to the replacement rack attachment portion, and FIG. 6 is a front view showing a state in which a filtration rack is attached to the replacement rack attachment portion. FIG. 7, FIG. 7 is a front view showing a state where a column purification rack is mounted on the replacement rack mounting portion, FIG. 8 is a fluid circuit diagram showing a piping system, FIG. 9 is an enlarged view showing a reaction tube and a sampling needle, and FIG. It is an enlarged view which shows a pipe | tube and a column refinement | purification needle.
[0010]
The compound automatic synthesizer 1 of this example has a transparent door 3 attached in front of a box-shaped main body 2. When the door 3 is opened, the base 4 of the main body 2 has a volume of 5 cc in the front left hand, for example. A reaction block 6 in which reaction tubes 5 and 5 are arranged is disposed, and an exchange rack mounting portion 7 for detachably mounting various racks is formed on the front right hand. Further, a robot arm 9 that is controlled to move in three orthogonal directions is disposed on the main body ceiling portion 8, and is arranged in the position of the reaction tube 5 arranged in the reaction block 6 or in the rack attached to the exchange rack attachment portion 7. The test tube is positioned so as to be positioned.
[0011]
The reaction tube 5 is provided with a cap 5b having an elastic packing 5a formed in a two-layer structure of silicon rubber and a fluororesin film at an upper end opening 59, and NS poles are formed at both ends inside thereof. The magnetic stir bar 5c is submerged.
More specifically, an aluminum cap 5b in which a through hole 56 through which chemical solution injection needles 50A and 50B described later are inserted is screwed into the upper end opening 59 of the reaction tube 5 and reacted with the aluminum cap 5b. An elastic packing 5 a is sandwiched between the upper end opening 59 of the tube 5.
This elastic packing 5a is formed of dimethyl silicon rubber 57 having a hardness HsA of about 20 to 25 measured with a JIS-A type hardness tester, and a lining 58 of a fluororesin film having a thickness of about 100 μm on the bottom surface thereof. Is given.
As this fluororesin film, for example, a film of tetrafluoroethylene-hexafluoropropylene copolymer is used.
[0012]
The reaction block 6 has, on its upper surface, array holes 10... For arranging, for example, reaction tubes 5 having a capacity of 5 cc in a matrix at predetermined intervals, and inside the reaction holes 6. In the state where 5... Are arranged, the stirring means 11 for forming a rotating magnetic field rotating in the circumferential direction of each reaction tube 5 to rotate the magnetic stirrer 5c submerged in the reaction tube 5, and each reaction tube 5 A temperature control means 12 for heating or cooling is provided.
In addition, a cooling manifold 13 is attached to the reaction block 6 for cooling the cap 5b portion during the heating reaction with the reaction tube 5 attached.
The reaction block 6 can also be removed from the automatic synthesizer 1 as necessary.
[0013]
The agitation means 11 has a pulley 14b attached to the other end of the rotary shaft 14 with a permanent magnet 14a disposed at the tip of each arrangement hole 10, and a plastic belt 14c is hung on each pulley 14b. The motor 15 is rotated and driven to rotate.
The temperature control means 12 includes an electric heater 16 that heats the reaction tubes 5 arranged in the arrangement holes 10 and a flow passage 17 that externally circulates a refrigerant that cools the reaction tubes 5. No. 16 is energized, or the refrigerant is circulated through the flow passage 17.
[0014]
Further, the cooling manifold 13 is attached to the reaction block 6 through a hinge 18 so as to be opened and closed, and a cooling block 20 having a recess 19 covering the cap 5b of each reaction tube 5 is arranged on the bottom surface side of the manifold 13. The cooling block 20 is formed with a flow passage 21 for circulating the refrigerant outside.
Each recess 19 is opened to the upper surface side through an insertion hole 22 into which a sampling needle (chemical solution injection needle) 50A described later is inserted, and the insertion holes 22 are two silicon rubbers arranged at a predetermined interval. The rubber sheets are closed by the sheets 23 </ b> A and 23 </ b> B, and the rubber sheets of the insertion holes 22 are communicated with each other via the nitrogen gas supply path 24.
[0015]
In the replacement rack mounting portion 7, a rack mounting surface 30 on which various racks are mounted is formed, and drain tanks 31 are formed on the left and right sides of the mounting surface 30.
The rack mounting surface 30 has a reagent rack 33 for arranging a plurality of test tubes 32 storing the reagents, a sample rack 35 for arranging test tubes 34 for storing the required amount of the finished compounds, and the finished compounds. A filtration rack 37 for arranging filtration cartridges 36 with a filter for injecting required amounts and filtering, a column purification rack 39 for arranging extraction tubes 38 for column purification of the compounds after the reaction, or compounds in the reaction and the reaction completion A TLC substrate support rack 61 that supports the TLC substrate 60 used for thin layer chromatography for analyzing the obtained compound substantially horizontally is formed to be mountable.
[0016]
The filtration rack 37 is configured such that a cartridge rack 42 in which filtration cartridges 36 are arranged at the same pitch as the test tubes 40 is detachably mounted on an upper surface of a rack body 41 in which the test tubes 40 are arranged. It is formed so that only part 36a can be exchanged.
[0017]
The column purification rack 39 is a shift in which a fraction rack 44 in which test tubes 43 are arranged is placed on a portion of the rack placement surface 30 where there is no drain tank 31 and an extraction tube 38 for column purification of a compound after completion of the reaction. A rack 45 is arranged to be slidable in the horizontal direction above the fraction rack 44. When the shift rack 45 is slid to one end side, the rack 45 is positioned directly above the fraction rack 44 and slid to the other end side. It is formed so as to be positioned right above the drain tank 31 when being operated.
[0018]
The TLC substrate 60 supported by the TLC substrate support rack 61 is formed by, for example, kneading a powder adsorbent such as silica gel, alumina, cellulose, etc. with a baked gypsum etc. and adhering it to a glass substrate or an aluminum substrate. A thin layer serving as a fixed layer is formed.
The TLC substrate 60 may be a single large sheet that can be placed on the substrate support rack 61, a relatively small one that is divided into a plurality of sheets, or a single large sheet. It may be formed so that it can be divided as required.
[0019]
Further, the robot arm 9 has a sampling needle (chemical solution injection needle) 50A for injecting a chemical solution into the reaction tube 5 or sucking the chemical solution from the reaction tube 5, and a compound or compound in the extraction tube 38 when performing column purification. A column purification needle (chemical solution injection needle) 50B for injecting a solvent is attached in a replaceable manner.
The sampling needle 50A is formed so as to be able to pierce and penetrate the elastic packing 5a attached to the cap 5b of the reaction tube 5, and a flow path 50a for inhaling and injecting a chemical solution is opened at the tip. An exhaust passage 50b for discharging the air in the reaction tube 5 to the outside with the needle 50A pierced is formed to open to the lower end side and the upper end side of the needle 50A.
In this case, when an acidic chemical solution such as hydrochloric acid or sulfuric acid is injected as the chemical solution, a sampling needle 50A in which at least a portion forming the flow path 50a is formed using an acid resistant metal such as Hastelloy C is used.
The column refining needle 50B has a spherical tip so as to be in close contact with the recess 38c of the cap 38b fitted into the upper end of the extraction tube 38 filled with the packing material 38a.
[0020]
The robot arm 9 includes a piping system 51 for inhaling and discharging a chemical solution and injecting a solvent. The piping system 51 has a port 52 connected to the needles 50A and 50B at one end thereof. A plurality of solvent bottles M _{1 to} M n are switched and connected to the other end via a switching valve CV, and a digital control syringe pump 53 is interposed as a flow control pump capable of switching the suction and discharge directions.
Therefore, by using this sampling needle 50A, a predetermined amount of reagent is sucked from the test tube 32 arranged in the reagent rack 33 and discharged into the reaction tube 5 arranged in the reaction block 6 by a predetermined amount, or a solvent bottle. A solvent is discharged from M _{1 to} Mn into the reaction tube 5 by a predetermined amount, or a compound that has finished the reaction in the reaction tube 5 is sucked into a test tube 34 arranged in a sample rack 35 or a filtration rack 37. Further, the filter cartridge 36 can be discharged.
Further, using the column purification needle 50B, a predetermined amount of a predetermined solvent is discharged from the solvent bottles M _{1 to} Mn to the extraction pipes 38 arranged in the column purification rack 39, or a required amount is obtained by inhaling the compound after the reaction. It can be discharged one by one.
[0021]
Reference numerals 54A and 54B denote needle holders for accommodating the needles 50A and 50B, and a cleaning device (not shown) for spraying cleaning liquid or pressurized air to the tips of the needles 50A and 50B is provided therein. ing.
Reference numeral 55 denotes a multiple pressurizing nozzle that pressurizes and fills nitrogen gas into the filtration cartridge 36 during filtration.
[0022]
The above is one configuration example of the present invention, and the operation thereof will be described next.
When synthesizing a compound, for example, (1) reagent injection operation, (2) solvent injection operation, (3) reaction operation, (4) TLC analysis operation, (5) liquid separation sampling operation, (6) filtration operation , (7) Since column purification operations are performed, these will be described.
[0023]
In the reagent injection operation, in order to synthesize a compound having an arbitrary mixing ratio for each reaction tube 5 arranged in the reaction block 6, the reagent stored in each test tube 32 arranged in the reagent rack 33 is inhaled. This is an operation of injecting a required amount into each reaction tube 5.
In this case, first, the reaction tube 5 is attached to the array hole 10 of the reaction block 6, and then the upper surface is covered with the cooling manifold 13. On the other hand, a reagent rack 33 in which a plurality of test tubes 32 storing the reagents is mounted on the replacement rack mounting portion 7. In this state, when the injection amount of the reagent is set for each reaction tube 5, the sampling needle 50 </ b> A is attached to the tip of the robot arm 9 to control the movement, and the reagent in the test tube 32 is sucked to each reaction tube 5. A predetermined amount is discharged at a time. Thereafter, the sampling needle 50A is cleaned by the cleaning device in the needle holder 54A, and then the reagent in the different test tube 32 is discharged to each reaction tube 5, and this is repeated, and a necessary type of reagent is supplied to the reaction tube 5 together. Inject at any mixing ratio.
[0024]
Next, a solvent injection operation is performed as necessary. In this case, when it is necessary to use a solvent for the synthesis of the compound, a predetermined amount of solvent is discharged into each reaction tube 5. In this case, the necessary solvent is removed from each of the solvent bottles M _{1 to} Mn by the sampling needle 50A. Is injected into each reaction tube 5.
Here, for example, when an acidic solvent such as hydrochloric acid or sulfuric acid is injected, an acid resistant needle having at least an internal flow path 50a made of an acid resistant metal is used as the sampling needle 50A.
[0025]
In this way, after injecting a plurality of reagents into the reaction tube 5 at an arbitrary mixing rate, the reaction operation is performed. In this method, predetermined temperature-time management is performed by stirring and temperature control, and the reaction is allowed to proceed under preset conditions.
In the case of stirring, if the motor 15 of the stirring means 11 is started, the permanent magnet 14a is rotated, so that a rotating magnetic field is formed for each array hole 10 and the magnetic stirring bar submerged in the reaction tube 5 5c rotates and the chemical | medical solution in the reaction tube 5 is stirred.
Further, when performing a predetermined temperature control, the heater 16 is energized when the set temperature is not lower than the room temperature, and when the set temperature is not higher than the room temperature, the refrigerant supplied from the outside circulates in the flow passage 17, The reaction proceeds under temperature conditions.
[0026]
When the reaction tube 5 is heated, the reaction solvent in the reaction tube 5 may leak to the outside and the reaction solution concentration may change.
Therefore, in this case, nitrogen gas is filled in the gap around the reaction tube 5 from the nitrogen gas supply path 24 formed in the cooling manifold 13 and the reaction tube 5 is placed under the nitrogen gas. The cap 5b of the reaction tube 5 is cooled by supplying a refrigerant. In particular, if an aluminum cap having excellent thermal conductivity is used as the cap 5b, the vicinity of the elastic packing 5a and the upper surface opening 59 in the reaction tube 5 is cooled by cooling the cap 5b.
As a result, the reaction solvent evaporated in the reaction tube 5 is cooled by the cap 5b, dewed and returned to the reaction tube 5, so that the concentration of the reaction solution in the reaction tube 5 does not change.
Further, since the reaction tube 5 is placed in a nitrogen atmosphere, there is no condensation on its periphery, particularly on the cap 5b. Even if condensation occurs, the upper surface opening 59 is airtight with the elastic packing 5a. Because it is blocked by water, water droplets do not enter inside.
An experiment in which 100% methanol is placed in the reaction tube 5 and the cap 5b is cooled for 24 hours using the elastic packing 5a of the present example in which the bottom surface of the silicon rubber 57 is provided with a fluororesin film lining 58 is used. As a result, it was confirmed that the reduction amount of the content averaged about 0.18%, and that a very good sealing property was obtained.
[0027]
Next, when the reaction is completed, a reaction terminator (for example, water) is injected to stop the progress of the reaction, and if necessary, it is confirmed whether or not the target compound is purified. In order to confirm the product in the reaction before completion of the TLC, a TLC analysis operation by thin layer chromatography is performed. This TLC analysis operation is particularly useful for confirming in which liquid layer the target compound is purified when the synthesized compound is separated into a plurality of liquid layers.
Specifically, the reagent rack 33 is removed, the TLC substrate support rack 61 is mounted, a required amount of a compound is dropped from each liquid layer onto the TLC substrate 60 supported by the rack 61, and the TLC substrate 60 After developing the sample, the sample is analyzed by irradiating with ultraviolet rays.
[0028]
And the target compound synthesize | combined in each reaction tube 5 is taken out. In this case, first, the reagent rack 33 or the TLC substrate support rack 61 mounted on the replacement rack mounting unit 7 is removed, and the sample rack 35 is mounted.
Here, when the compound synthesized in the reaction tube 5 is colloidal and not separated into a plurality of liquid layers, there is no need to determine the position of the liquid layer. What is necessary is just to inhale with the sampling needle 50A.
In addition, when the synthesized compound is separated into a plurality of liquid layers, liquid separation sampling is performed, and a chemical solution in the liquid layer portion where the target compound is purified is collected. This is because, by setting the position of the liquid layer in the reaction tube 5, the tip of the sampling needle 50A is positioned in the liquid layer and inhaled, and the inhaled compound is discharged to the test tube 34 of the sample rack 35. This is performed for each reaction tube 5.
[0029]
Furthermore, when it is necessary to filter the compound for which the reaction has been completed, the filtration rack 37 is mounted so as to be positioned immediately above the drain tank 31 of the replacement rack mounting portion 7. And when filtering the compound extract | collected in the test tube 34 of the sample rack 35, the sample rack 35 is mounted on the reaction block 6, and the compound in the test tube 34 is inhaled using the sampling needle 50A. Inject into the filtration cartridge 36. When the compound is directly injected from the reaction tube 5 into the filtration cartridge 36, the compound in the reaction tube 5 is sucked into the filtration cartridge 36 using the sampling needle 50 </ b> A.
Next, if necessary, the filtration time can be shortened by supplying pressurized nitrogen gas into the filtration cartridge 36 using the multiple pressure nozzles 55.
[0030]
In the case where a column purification operation is performed as a pretreatment for structural analysis of the synthesized compound, the column purification rack 39 is mounted on the exchange rack mounting portion 7.
In this case, first, the test tube 43 is arranged in the fraction rack 44, the extraction tube 38 is arranged in the shift rack 45, and the shift rack 45 is slid to the drain tank 31 side. In this state, the column purification needle 50B is attached to the tip of the robot arm 9, and a predetermined solvent is injected into each extraction tube 38 for conditioning.
[0031]
Next, when the conditioning is completed, sample loading is performed in which the sample sucked from the sample rack 35 placed on the reaction block 6 is injected into each extraction tube 38. In this case, every time one sample is injected into one extraction tube 38, the tip of the column purification needle 50B is washed with the washing device in the needle holder 54B, thereby preventing the samples from being mixed.
[0032]
When the sample loading is completed, a washing operation is performed to wash away foreign substances held in the fixed layer of the extraction tube 38. This is performed by injecting a cleaning solvent with the column purification needle 50B, and the liquid flowing out from the extraction tube 38 at that time is recovered in the drain tank 31.
[0033]
When the washing operation is completed, the shift rack 45 is moved to the fraction rack 44 side, and a predetermined solvent is injected into the extraction tube 38, whereby the eluate is collected in each test tube 43 of the fraction rack 44 to perform column purification operation. Exit.
In this case, the eluate in the extraction tube 38 is not directly collected in the test tube 43, but the eluate is monitored with an ultraviolet absorptiometer to detect the substance contained in the eluate and the concentration of the eluate, Each time the detected substance or concentration changes, the fraction rack 44 may be moved and automatically replaced with a new test tube 43, and automatic fractionation may be performed using only the ultraviolet absorption peak as a fraction.
Then, the eluate collected in each test tube 43 of the fraction rack 44 is concentrated by a centrifugal separator, dissolved, and then subjected to structural analysis if applied to a structural analyzer.
[0034]
【The invention's effect】
As described above, according to the present invention, only by exchanging the rack mounted on the replacement rack mounting portion, reagent injection operation, synthesis reaction operation, liquid separation sampling operation, filtration operation, column purification operation, etc. Each operation necessary for the parallel synthesis can be automatically performed by one apparatus, and the apparatus itself can be formed in a very compact manner, and the manufacturing cost can be reduced.
[Brief description of the drawings]
FIG. 1 is a front view showing an apparatus for synthesizing a compound according to the present invention.
FIG. 2 is a sectional view taken along line II.
FIG. 3 is an enlarged view showing an internal structure of a reaction block.
FIG. 4 is a front view showing a state in which a sample rack is mounted on the replacement rack mounting portion.
FIG. 5 is a front view showing a state in which a TLC substrate support rack is mounted on the replacement rack mounting portion.
FIG. 6 is a front view showing a state in which a filtration rack is mounted on the replacement rack mounting portion.
FIG. 7 is a front view showing a state in which a column purification rack is mounted on the replacement rack mounting portion.
FIG. 8 is a fluid circuit diagram showing a piping system.
FIG. 9 is an enlarged view showing a reaction tube and a sampling needle.
FIG. 10 is an enlarged view showing an extraction tube and a column purification needle.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Automatic compound synthesizer 5 ... Reaction tube 5a ... Elastic packing 5b ... Cap 5c ... Magnetic stirrer 6 ... Reaction block 7 ... Replacement rack mounting part 11 ... Stirring means DESCRIPTION OF SYMBOLS 12 ... Temperature control means 16 ... Heater 17 ... Flow path 30 ... Rack mounting surface 31 ... Drain tank 32 ... Test tube 33 ... Reagent rack 34 ... Test tube 35 ... Sample rack 36 ... Filtration cartridge 37 ... Filtration rack 38 ... Extraction tube 39 ... Column purification rack 43 ... Test tube 44 ... Fraction rack 45 ... Shift rack 50A ..Sampling needle (chemical solution injection needle)
50B ・ ・ Column purification needle (chemical injection needle)
56 ... Through hole 57 ... Silicon rubber 58 ... Lining 59 ... Upper end opening 60 ... TLC substrate 61 ... TLC substrate support rack

Claims (5)

Chemical solution injection needles (50A, 50B) that are movable and controlled so as to be positioned at the positions of the test tubes (32) arranged in the reagent rack (33) and the positions of the reaction tubes (5) arranged in the reaction block (6). ), The reagent stored in each test tube (32) is aspirated and injected into each reaction tube (5) in a required amount, and a compound having an arbitrary mixing ratio is added to each reaction tube (5). In an automatic synthesizer for compounds to be synthesized,
The reaction block (6) includes a stirring means (11) for rotating a magnetic stirring bar (5c) submerged in the reaction tube (5) by forming a rotating magnetic field rotating in the circumferential direction of each reaction tube (5). And a temperature control means (12) for heating or cooling each reaction tube (5), and the reagent rack (33) is detachably attached to the replacement rack attachment part (7),
In place of the reagent rack (33), the replacement rack mounting part (7) replaces the reagent rack (33) with a sample rack (35) in which test tubes (34) for storing the required amount of the compound after the reaction are arranged, and the reaction completed compound. A filtration rack (37) in which filtration cartridges (36) with filters for injecting required amounts and filtering are arranged, or a column purification rack (39) in which extraction tubes (38) for purifying the compounds after the reaction are arranged. An automatic compound synthesizer characterized in that either one is formed so as to be wearable.   The temperature control means (12) of the reaction block (6) includes a heater (16) for heating each reaction tube (5) and a flow passage (17) for externally circulating a refrigerant for cooling each reaction tube (5). An automatic synthesis apparatus for a compound according to claim 1.   2. The compound automatic synthesizing apparatus according to claim 1, wherein the exchange rack mounting portion (7) is formed with drain tanks (31) on left and right sides of a rack mounting surface (30) on which a rack is mounted.   In the replacement rack mounting portion (7), a drain tank (31) is formed on one side of the rack mounting surface (30) on which the rack is mounted, and the column purification rack (39) is connected to the extraction tube (38). The fraction rack (44) in which the test tubes (43) for collecting the eluate from the column are placed on a portion of the rack placement surface (30) where there is no drain tank (31), and the reaction-completed sample is A shift rack (45) in which extraction tubes (38) for column purification are arranged is slidable in the horizontal direction above the fraction rack (44), and the shift rack (45) is slid to one end side. 2. It is formed so as to be positioned above the fraction rack (44) when being moved and positioned above the drain tank (31) when being slid to the other end side. Automatic synthesizer of the compound of the mounting.   In place of the reagent rack (33), the exchange rack mounting portion (7) is mounted with a TLC substrate support rack (61) that supports a TLC substrate (60) on which a fixed layer for thin layer chromatography is formed substantially horizontally. The automatic compound synthesizer according to claim 1, wherein the compound synthesizer is capable of being formed.

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