JP3454487B2 - Milk-derived calcium-containing composition and method for producing the same - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a milk-derived calcium-containing composition, a milk-derived calcium agent, a method for producing the same, and foods, feeds and pharmaceuticals containing them. The milk-derived calcium-containing composition of the present invention is a composition consisting of inorganic salts containing calcium as a main component obtained by baking casein-binding calcium and / or colloidal calcium contained in milk at a high ratio, High bioavailability.

[0002]

2. Description of the Related Art At present, the calcium intake of Japanese people has been lower than the nutritional requirement for many years. However, it is difficult to ingest a sufficient amount of calcium according to the average dietary habits of the Japanese people, and considerable effort is required to continue a menu that allows daily intake. Therefore, intake of a calcium agent having good calcium absorbability is desired. On the other hand, it has been reported that calcium in milk has good absorbability. There are three forms of milk calcium: casein-binding calcium, colloidal calcium and soluble calcium. Casein-binding calcium is calcium bound to casein micelles, and colloidal calcium is in the form incorporated into casein micelles in the form of calcium apatite. Also, 1/3 of the soluble calcium is ionic, but the rest is in the form of calcium citrate. Regarding the absorbability of calcium in milk by form, it is reported that the absorbability of soluble calcium is excellent, and further, the absorbability of casein-binding calcium and colloidal calcium is excellent (Nutr. Rep.Int., 21,6738
(1980)). And about 60 of calcium contained in milk
70% is in the form of casein-binding and colloidal calcium.

Conventionally, whey obtained by precipitating casein under acidic conditions, or whey obtained by precipitating casein with rennet or the like has been used as a technique for obtaining milk minerals. That is, the conventional method of obtaining minerals from milk has been extraction and crystallization that ignores the forms of casein-binding calcium and colloidal calcium present in the casein fraction of milk, but these are not burned. However, it contains whey protein and lactose and is not always desirable for the purpose of calcium supply. Therefore, the present inventors have previously filed a patent application for a calcium agent in which calcium bound to casein is selectively collected and a method for producing the same (Japanese Patent Laid-Open No. H06-69242).
-125740).

[0004]

DISCLOSURE OF THE INVENTION The present invention solves the problems of the prior art as described above, and a milk-derived calcium-containing composition containing casein-binding calcium and / or colloidal calcium present in a milk casein fraction. An object of the present invention is to provide a method for producing a product, and a milk-derived calcium-containing composition produced by the method and having excellent absorbability. As a result of intensive studies conducted by the present inventors, by baking a milk-derived preparation containing casein-binding calcium and / or colloidal calcium in the milk casein fraction, the milk-derived preparation highly efficiently absorbed was derived. The inventors have found that a calcium-containing composition can be produced, and completed the present invention.

[0005]

SUMMARY OF THE INVENTION Accordingly, the present invention provides a milk
Made from milk-derived calcium-containing composition comprising calcium obtained by baking a milk casein binding calcium were isolated from casein and / or colloidal calcium inorganic salts as the main component. The present invention also comprises a milk-derived calcium agent obtained by baking a casein fraction containing milk casein-binding calcium and / or colloidal calcium. The present invention also comprises a method for producing a milk-derived calcium-containing composition, which comprises separating and recovering casein-binding calcium and / or colloidal calcium from milk casein, and then calcining it. The present invention also comprises a food, a feed, or a medicament for preventing or treating osteoporosis and various bone diseases, characterized by containing the milk-derived calcium-containing composition. The present invention also comprises a medicament for preventing or treating osteoporosis and various bone diseases, which comprises the milk-derived calcium agent as an active ingredient.

[0006]

The present invention is described in detail below. Examples of milk used as a raw material in the present invention include milk of mammals such as milk, human milk, goat milk, buffalo milk and sheep milk, and skim milk obtained by defatting the milk. In the production of the milk-derived calcium-containing composition of the present invention, a milk-derived casein fraction is obtained, or casein-binding calcium and / or colloidal calcium is separated from milk casein to obtain a milk-derived preparation. Good. The method for obtaining the casein fraction of milk is not particularly limited,
For example, it can be obtained by rennet treatment at pH 5.5 or higher. Also, by the operation to obtain casein,
It is also possible to easily remove lactose, whey protein and the like. Further, the method for separating casein-binding calcium and colloidal calcium from casein is not particularly limited, but, for example, inorganic acids such as hydrochloric acid and acetic acid, lactic acid,
Gradually add organic acid such as citric acid and the pH will be 5.4.
When it becomes below, the bond of casein-binding calcium that had been bound to casein begins to be cleaved, and when the pH becomes 4.8, it is completely separated from casein. In this process, colloidal calcium is separated when the pH reaches about 5.2. Therefore, to obtain colloidal calcium, a pH of about 5.2
In order to obtain both casein-binding calcium and colloidal calcium,
The recovery process may be performed at about 4.7 to 4.5. Since casein remains insoluble, casein-binding calcium and / or colloidal calcium can be easily recovered by collecting the supernatant fraction.

Next, the milk-derived preparation containing casein-binding calcium and / or colloidal calcium thus obtained is calcined. The calcining is carried out by means of spray drying or the like. After drying, 45
It can be performed by heating at a temperature of 0 ° C. or higher. In this way, the milk-derived calcium-containing composition of the present invention is obtained. The milk-derived calcium-containing composition of the present invention contains calcium as a main component, as well as phosphorus, as shown in Example 1 below. It is composed of inorganic salts such as magnesium, potassium, sodium, iron and zinc. The present invention also includes a milk-derived calcium agent containing only calcium contained in this composition.

The milk-derived calcium-containing composition of the present invention is
It can be contained in foods, feeds and medicines. Examples of foods include various beverages; dairy products such as cheese and yogurt; confectionery such as jellies, cookies and biscuits; bread; noodles such as buckwheat noodles, udon and spaghetti; soups; processed meat products such as sausage and ham. . In these foods, as the raw materials other than the milk-derived calcium-containing composition of the present invention, those commonly used can be used.
The milk-derived calcium-containing composition of the present invention can be used by dispersing, mixing or dissolving it in a raw material. The content of the milk-derived calcium-containing composition of the present invention is not particularly limited, but it is usually preferable that the content of the milk-derived calcium-containing composition is 0.1 to 10% by weight in the food. The food containing the milk-derived calcium-containing composition of the present invention can be used as a functional food or a health food.

Examples of the feed include pet foods such as dogs, cats and small birds, as well as feeds for domestic animals such as horses, cows and pigs, and poultry such as chickens and pheasants. In these feeds, as the raw materials other than the milk-derived calcium-containing composition of the present invention, those commonly used can be used. The content of the milk-derived calcium-containing composition of the present invention does not need to be particularly limited, but normally, it is preferable that it is contained in the feed in an amount of 0.1 to 2% by weight. The feed containing the milk-derived calcium-containing composition of the present invention is useful for treating and preventing various bone diseases of animals and for improving the health condition.

Further, as a pharmaceutical, various orally administrable dosage forms such as tablets (tablets), granules, powders, pills, dispersions, suspensions, etc., alone or together with excipients and additives, etc. Can be The milk-derived calcium-containing composition or the milk-derived calcium agent of the present invention can be used as a therapeutic or prophylactic agent for osteoporosis, various bone diseases and the like. Further, the dose of the milk-derived calcium-containing composition or milk-derived calcium agent of the present invention, the symptoms of the patient, age,
Although it is determined according to body weight, sex, etc., it is generally preferable to administer 1 to 50 mg / kg per day to an adult once a day or in several divided doses.

[0011]

EXAMPLES The present invention will be described in more detail with reference to Examples and Test Examples. Example 1 (Method for producing calcium-containing composition derived from milk) 20 L of raw milk
Is skimed by centrifugation at 5,000 xg for 10 minutes to obtain 9.5 L skim milk.
Got Calf rennet was added to this so as to be 0.03%, and left at 30 ° C. for 1 hour to coagulate casein. The casein was collected by centrifugation at 5,000 xg for 10 minutes. The casein was washed by suspending it in double distilled water and centrifuging, and then 10% of the casein was freeze-dried as calcium-containing casein. Further, the casein obtained by washing was divided into 45% portions and suspended in 1,000 ml of distilled water, and then one was adjusted to pH 5.2 and the other was adjusted to pH 4.6.
After stirring for 10 minutes, the mixture was centrifuged at 5,000 xg for 10 minutes to collect casein in the precipitated fraction, selectively collect colloidal calcium and casein-binding calcium in the supernatant, concentrate under reduced pressure with a rotary evaporator, and freeze-dry. . Then, it was baked in an electric furnace at 550 ° C. to obtain a milk-derived calcium-containing composition. The yield obtained by adjusting the pH to 5.2 was 12.2 g (A), and the yield (B) obtained by adjusting the pH to 4.6 was 16.3 g. The results of analyzing each component are shown in Tables 1 and 2 below. As shown in Table 1, the obtained milk-derived calcium-containing compositions (A) and (B) were both inorganic salts containing calcium as a main component. Moreover, milk-derived calcium-containing composition (A)
As a result of measuring the proteins, lipids, sugars and fibers of (B) and (B), none was detected as shown in Table 2.

[0012]

[Table 1]       Components of milk-derived calcium-containing compositions (A) and (B) (% by weight)       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−                                A B       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−          Moisture 6.35 5.02          Calcium 47.74 51.04          Phosphorus 19.00 21.04          Magnesium 2.56 2.05          Potassium 0.34 1.06          Sodium 1.52 1.04          Iron (mg%) 4.1 5.19          Zinc (mg%) 3.0 2.13       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−

[0013]

[Table 2]       Components of milk-derived calcium-containing compositions (A) and (B) (% by weight)       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−                                A B       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−          Protein 0.00 0.00          Lipid 0.00 0.00          Fiber 0.00 0.00       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−

Test Example 1 (Absorption Experiment by Oral Administration to Rats) A calcium absorption test was carried out using 10-week-old SD male rats. 1
Milk-containing calcium-containing compositions (A) and (B) obtained in Example 1 as a group of 6 animals, uncalcined milk-derived calcium agent obtained in Example 2 of JP-A-6-125740, and milk A calcium absorption experiment was carried out as follows with respect to clear calcium (Whey Ca) and dicalcium phosphate. Calcium content of each sample is 1
Each diet containing 0 mg / ml was orally administered by a sonde to rats fasted for 24 hours. At this time, phosphorus and magnesium were made to have the same composition, and protein and sugar were also made to have the same composition by purified casein and lactose, respectively. After 4 hours, the stomach and intestines were extracted and ashed as they were. The amount of digestive absorption was determined by subtracting the amount of residual calcium in the stomach and intestine from the amount of administered calcium. As a result, the milk-derived calcium-containing compositions (A) and (B) of Example 1 were obtained from the uncalcined milk-derived calcium agent and whey-derived calcium (Whey) according to JP-A-6-125740.
When compared statistically with Ca) and dicalcium phosphate as shown in Table 3 below and FIG. 1, it was found that the risk rate was 5% and that there was a significant difference from any other calcium. (N = 6), it showed very high absorbency. Therefore, the colloidal calcium and casein-binding calcium of Example 1 are considered to have high bioavailability.

[0015]

[Table 3] Calcium absorption         Calcium-containing sample Calcium absorption (% by weight)       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−       Milk-derived calcium-containing composition (A) 2.51 ± 0.16       Milk-derived calcium-containing composition (B) 2.54 ± 0.23       Non-baked milk-derived calcium agent 2.21 ± 0.15         (JP-A-6-125740)       Whey Ca 1.85 ± 0.21       Dicalcium phosphate 1.45 ± 0.35       −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−

Example 2 (Production of fruit juice) An apple juice drink was produced in the following mixing ratio. Apple juice was prepared by mixing the above materials except the milk-derived calcium-containing composition (A). To this, the milk-derived calcium-containing composition (A) obtained in Example 1 was added,
The mixture was sterilized for 30 minutes to obtain a calcium-fortified apple juice beverage. The obtained apple fruit juice was comparable in flavor and texture to fruit juice drinks containing no calcium agent and conventional fruit juice drinks containing calcium agent.

Example 3 (Production Method of Jelly) A jelly was produced by the usual production method with the following compounding ratio. Fruit juice 20% by weight, granulated sugar 15.0% by weight, starch syrup 5.0% by weight, agar powder 1.0 in 58.4% by weight of water at 40 ° C.
% By weight, 0.5% by weight of the milk-derived calcium-containing composition (B) obtained in Example 1 and 0.1% by weight of a fragrance were mixed to form a mix. After sterilizing this mix for 2 seconds at 130 ° C, it is filled in a container by a filling machine and further stored in a refrigerator.
Cooled to ℃ and obtained jelly. Flavor of the obtained jelly,
The texture and taste were comparable to those of the jelly without the addition of the calcium agent and the jelly added with the conventional calcium agent.

Example 4 (Production of milk powder) 125 g of the milk-derived calcium-containing composition (A) obtained in Example 1 was dissolved in 2 kg of warm water at 60 ° C.,
It was added to 25 kg of raw milk and stirred. This was sterilized at 120 ° C. for 2 seconds, concentrated with a concentrator, and then spray-dried to obtain 3 kg of calcium-fortified milk powder. Further, this powdered milk was granulated with a fluidized bed granulator to a particle size of 200 to 350 μm.

Example 5 (Production of cheese) 34 kg of cheddar cheese and 200 kg of gouda cheese were crushed and mixed, and 1.1 kg of molten salt (mixed molten salt consisting of 80% sodium polyphosphate and 20% sodium diphosphate) and The milk-derived calcium-containing composition (B) (1.1 kg) obtained in Example 1 was added, and the mixture was heated and melted in a Stefan cutter (750 rpm) to 85 to 90 ° C., then filled and cooled. The obtained calcium-fortified processed cheese was comparable in flavor, texture, and taste to cheese without addition of a calcium agent and cheese containing a conventional calcium agent.

Example 6 (Production of tablet) 220 g of the milk-derived calcium-containing composition (A) obtained in Example 1 and corn starch 163.
g, 10 g of crystalline cellulose and 6.8 g of calcium carboxymethyl cellulose were mixed with a kneader, and then kneaded while spray-dripping 50 ml of water. 20 of the kneaded mixture
The mixture was granulated with a uniaxial oscillator equipped with a mesh screen and dried with a fluidized bed dryer. The dried product was crushed and sized with a flash mill to obtain a tableting powder. 8 g of a sucrose fatty acid ester as a lubricant was mixed therein with a V-type mixer, and the mixture was tableted with a tableting machine having a punch having a diameter of 11 mm set to give tablets having an average weight of 0.35 g. The composition of this tablet is as follows: Milk-derived calcium-containing composition 55
% By weight, corn starch 40.8% by weight, crystalline cellulose 2.5% by weight, carboxymethylcellulose 1.7% by weight.

Example 7 (Production of dog breeding feed (dog food))

Mineral mixture CaCO ₃ 3.0 g KH ₂ PO ₄ 2.0 g NaH ₂ PO ₄ 1.5 g MgO 0.5 g MnCO ₃ 40.0 mg FeC ₆ H ₅ O ₇ 30.0 mg 70% ZnO 10.0 mg 55% CaCO ₃ 4.5 mg KIO ₃ 0.65 mg Na ₂ SeO ₃ / 5H ₂ O 0.05 mg CrK (SO ₄ ) / 12H ₂ O 5.0 mg −−−−−−−−−−−−−−−−−−−−− Sucrose was added to 9 g. .

Vitamin mixture Vitamin A 1500 IU Vitamin D ₃ 300 IU Vitamin E 6.8 mg Vitamin B ₁ 0.9 mg Vitamin B ₂ 0.4 mg Vitamin B ₆ 0.5 mg Vitamin B ₁₂ 3.4 mg Vitamin C 50.0 mg Pantothenic acid 4.0 mg Folic acid 0.2 mg Choline 200.0 mg Biotin 24.4 μg Inositol 50.0 mg Niacin 10.5 mg −−−−−−−−−−−−−−−−−−−−−−−- Sucrose was added to make 2 g.

The above-mentioned mineral mixture and vitamin mixture were added to 100 g of the above-mentioned basic dog feed, and a dog feed was produced by a conventional method.

[0025]

The milk-derived calcium-containing composition and the milk-derived calcium agent of the present invention have a high calcium content, excellent absorbability of calcium, high bioavailability, and do not contain lactose or whey protein. , Easy to use. Further, since the milk-derived calcium-containing composition and the milk-derived calcium agent of the present invention do not contain components other than inorganic salts such as proteins and sugars, there is no fear of allergies due to these proteins or sugars. There are advantages. Moreover, according to the manufacturing method of this invention, the milk-derived calcium-containing composition of this invention can be manufactured highly efficiently and at low cost. Furthermore, the food, feed and medicine containing the milk-derived calcium-containing composition of the present invention and the medicine containing the milk-derived calcium agent of the present invention are useful for supplementing human or animal calcium. Therefore, the food of the present invention, as a functional food and health food, the pharmaceutical of the present invention,
As a therapeutic agent and preventive agent for various osteoporosis and bone diseases, the feed of the present invention is extremely useful for improving the health condition of animals and treating and preventing various bone diseases.

[Brief description of drawings]

Figure 1: Whey-derived calcium (Whay Ca), phosphate No. 2
Calcium (CaHPO _4), is a graph showing the calcium absorption of the fired not derived from milk calcium agent (JP-A-6-125740) and milk-derived calcium-containing composition of the present invention (A) and (B).

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI A61P 19/08 A61P 19/08 19/10 19/10 (56) Reference JP-A-6-125740 (JP, A) JP Flat 5-339160 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB name) A23C 9/14 A23K 1/175 A23L 1/304 A61K 33/06 A61K 35/20

Claims (7)

(57) [Claims] 1. A milk casein binding calcium were isolated from casein milk and / or colloidal calcium derived from milk calcium-containing composition consisting of inorganic salts mainly comprising calcium obtained free form baked <br/> . 2. A milk-derived calcium agent obtained by baking a casein fraction containing milk casein-binding calcium and / or colloidal calcium. 3. Casein-binding calcium and / or colloidal calcium is separated and recovered from milk casein,
Next, a method for producing a milk-derived calcium-containing composition, which comprises firing it. 4. A food comprising the milk-derived calcium-containing composition according to claim 1. 5. A feed comprising the milk-derived calcium-containing composition according to claim 1. 6. A pharmaceutical composition for preventing or treating osteoporosis and various bone diseases, which comprises the milk-derived calcium-containing composition according to claim 1 as an active ingredient. 7. A pharmaceutical composition for preventing or treating osteoporosis and various bone diseases, which comprises the milk-derived calcium agent according to claim 2 as an active ingredient.