JP2824491B2 - Drug release filter jig - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention is to release a small amount of a drug or the like into a solution to cause the drug to act on a sample such as cells in the solution, and to filter the solution. The present invention relates to a drug release / release filtration jig for capturing a sample.

[Conventional technology]

When microscopically examining a specimen such as a cell, it is necessary to prepare a preparation in advance, but this preparation has conventionally been prepared by a so-called smearing method. However, in this smearing method, a specimen is painted on a slide glass by a technique, so that a physical force acts on the specimen and the specimen is easily deformed. In addition, the specimen is easily detached and detached from the slide glass at the time of staining, so that a high degree of skill is required.

Conventionally, for example, the efficacy of a newly developed drug on a specimen such as a cell is confirmed by IN-VITRO (in a test tube) or IN-VIVO (in a living body). In such a conventional method, cells in a test tube or cells of a living body are stained with a living body and then microscopically examined as a preparation by a smear method.

[Problems to be solved by the invention]

The present inventor has previously invented a filtration jig (Japanese Patent Application No. 1-179664) in order to solve the problems in the conventional smearing method. In this method, a filter having a myriad of micropores is held by a filtration jig, the solution is filtered by this filter, and only the specimen in the solution is captured on the filter.
Then, without smearing, the filter is placed on a slide and the specimen is examined microscopically.

In the invention of the above-mentioned filtration jig, if the same filtration jig can also achieve the action of various chemicals and the like on the sample, it is convenient because there is no need to use a smearing method requiring skill. The present application pays attention to such a point, and an object thereof is to provide a jig capable of easily and accurately performing a series of steps such as sensitivity of a drug or the like to a specimen and capture and staining of the specimen.

[Means for solving the problem]

As means for this purpose, an injection body 1, a filter body 2, a medicine container 3, and a jig main body 4 were constituted.

The injection body 1 has a cylindrical shape into which a solution 7 containing a sample 13 is injected, the filter body 2 has a frame 5 on which a filter 6 having an infinite number of micropores is stretched, and the medicine container 3 has a medicine 9.
And the upper opening is sealed with a semi-permeable membrane 8.

The jig body 4 has a filter hole 10 penetrating in the vertical direction at least at the center, and a medicine container on the upper surface in order from the bottom.
3. The filter body 2 and the injection body 1 are detachably held in contact with the semipermeable membrane 8 of the medicine container 3.

It is preferable that the medicine container 3 be rotatable or half-turnable by the rotating device 12.

A communication hole 11 communicating with the filtration hole 10 is provided on a side surface of the jig body 4.
Can be formed.

[Action]

In the above configuration, first, a specimen such as a cell is placed in the injection body 1.
Solution 7 containing 13 is injected. Since the lower end opening of the injection body 1 is in contact with the filter 6 of the filter body 2 and the filter 6 is in contact with the semipermeable membrane 8 of the medicine container 3, the solution 7 in the injection body 1 is filled with the semipermeable membrane 8 and The filter 6 is filtered and filtered into the medicine container 3, while the medicine 9 in the medicine container 3 is similarly released through the semipermeable membrane 8 and the filter 6 and released into the injecting body (a small amount is released). This is because the semipermeable membrane 8 has a property of allowing a small amount of liquid (solvent) to permeate over time, and the filter 6 has countless micropores. The drug 9 mixed in the solution 7 in the injection body 1 gradually acts on the sample 13.

After a certain period of time, the medicine container 3 is removed, and the solution 7 in the injection body 1 is filtered by suctioning through the filtration hole 10.
The sample 13 in the solution 7 is captured on the filter 6 (the solution 7 can be suctioned from the gap between the drug container 3 and the jig main body 4 while the drug container 3 is mounted. The filter 6 capturing 13 is placed on the slide,
What to do microscopic examination.

When a plurality of medicines 9 are desired to act on the sample 13, the medicine containers 3 containing the medicines 9 may be sequentially attached.

Also, by rotating the medicine container 3 by the rotating device 12, the medicine 9 is evenly supplied into the solution 7 in the injecting body, so that the medicine 9 acts on all the specimens 13 and thus the medicinal effect of the medicine 9 is reduced. You can know.

Further, by providing the communication hole 11 on the side surface of the jig main body 4, oxygen, carbon dioxide, a culture solution, and the like necessary for culturing the specimen 13 in the solution 7 can be injected from the communication hole 11. By culturing the specimen 13 for a long time, the medicinal effect of the drug 9 can be observed over time.

〔Example〕

FIG. 1 shows an embodiment of the present invention. This is a basic form of the jig of the present invention.

The jig main body 4 has a substantially cubic shape, and the medicine container 3, the filter body 2, and the injection body 1 are densely assembled on the upper surface in this order from the bottom. The jig body 4 has a filter hole 10 in the vertical direction and a communication hole 11 communicating with the filter hole 10 in the horizontal direction. Filter hole
Reference numeral 10 denotes a portion for applying a suction force when filtering the solution 7 and a portion into which the rotating rod 20 of the rotating device 12 enters when rotating the medicine container 3. When the solution 7 is suctioned and filtered through the filtration hole 10, the communication hole 11 is sealed with a stopper 16. The communication hole 11 is used for filtering the solution 7 similarly to the filtration hole 10, and is used for filtering the solution 7 of the injection body 1.
It is used to supply necessary elements to the specimen 13 such as oxygen, carbon dioxide, culture solution and the like. When sending oxygen or the like from the communication hole 11, the filtration hole 10 is sealed with the stopper 16, the medicine container 3 is removed, and the injection device 19 is inserted into the communication hole 11.

The medicine container 3 is constructed by extending a semipermeable membrane 8 at an opening on the upper surface of a thin container, and has an engaging portion 15 formed on the bottom surface thereof. When the medicine container 3 is rotated,
The assembling part 14 of the rotating device 12 is to be assembled. Rotating device 12
Can be operated by motor or manually.

The injection body 1 is provided with a bottle 17 via a tube 18.
The solution 7 increased by the culture solution or the like injected by the injection device 19 is stored in the bottle 17 through the tube 18.

The solution 7 can be automatically filtered through a communication hole 11 after being filtered by a filtration device (not shown).

FIG. 2 and FIG. 3 show an example of the medicine container 3. The medicine container 3 shown in FIG. 2 contains one kind of medicine 9. The medicine container 3 shown in FIG. 3 contains a plurality of kinds (four kinds) of medicines 9. The four types of medicines 9 are stored independently of each other, and each is covered with a permeable membrane 8 having a different transmission speed. By doing so, the release time of each drug 9 can be controlled and the cell killing effect of the concomitant drug can be known.

〔The invention's effect〕

As described above, according to the present invention, a drug or the like can be easily released and acted on a specimen such as a cell, and a specimen on which the drug or the like has acted can be reliably captured on a filter. Specimens can be microscopically examined simply by placing a filter on a slide, eliminating the need for conventional techniques such as smearing, and simplifying the one-step preparation preparation process. .

[Brief description of the drawings]

FIG. 1 is a front sectional view showing one embodiment of the present invention, FIG. 2 is a plan view showing one example of a medicine container, and FIG. 3 is a plan view showing another example. Explanation of symbols 1: injection body, 2: filter body, 3: drug container, 4: jig body, 5: frame body, 6: filter, 7: solution, 8: semipermeable membrane, 9: drug, 10: filtration Hole, 1
1: Communication hole, 12: Rotating device, 13: Sample, 14: Assembling part, 15: Engaging part, 16: Stopper, 17: Bottle, 18: Tube, 19: Injection instrument, 20: Rotating rod.

Continued on the front page (58) Fields surveyed (Int.Cl. ⁶ , DB name) G01N 33/48-33/52 G01N 33/58-33/98 G01N 33/15 G01N 1/28

Claims (3)

(57) [Claims] 1. A cylindrical injection body (1) into which a solution (7) containing a sample (13) is injected, and a filter (6) having a myriad of micropores stretched over a frame (5). It has a filter body (2), a medicine container (3) for accommodating a medicine (9) and having an upper opening closed with a semipermeable membrane (8), and a filtration hole (10) penetrating at least in the center in the vertical direction. At the same time, the medicine container (3),
A drug release comprising a filter body (2) in contact with the semipermeable membrane (8) of the drug container (3), and a jig body (4) for detachably holding the injection body (1). Filtration jig. 2. The jig for filtering and releasing medicine according to claim 1, wherein the medicine container (3) is rotatable or half-turnable by a rotating device (12). 3. The jig according to claim 1, wherein a communication hole (11) communicating with the filtration hole (10) is formed on a side surface of the jig body (4).