JP2569879B2 - Suction leachate collection device, suction leachate amount measurement device, and biological related substance measurement device - Google Patents
Suction leachate collection device, suction leachate amount measurement device, and biological related substance measurement deviceInfo
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- JP2569879B2 JP2569879B2 JP2070101A JP7010190A JP2569879B2 JP 2569879 B2 JP2569879 B2 JP 2569879B2 JP 2070101 A JP2070101 A JP 2070101A JP 7010190 A JP7010190 A JP 7010190A JP 2569879 B2 JP2569879 B2 JP 2569879B2
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- Prior art keywords
- suction
- leachate
- substance
- suction leachate
- diluent
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Description
【発明の詳細な説明】 （産業上の利用分野） 本発明は、生体関連物質を非侵襲的に採取する吸引浸
出液の採取装置、及びこれを利用した吸引浸出液量測定
装置、生体関連物質測定装置に関する。Description: TECHNICAL FIELD The present invention relates to a suction leachate collecting apparatus for non-invasively collecting a biological substance, a suction leachate amount measuring apparatus using the same, and a biological substance measuring apparatus. About.
（従来の技術） 従来、生体関連物質の測定は、人体から血液を採取
し、これを臨床検査装置によって分析することにより行
なわれている。しかし、採血は、必要成分の分離にいく
つかの操作が必要であり、人体への侵襲や感染の心配が
ある。(Prior Art) Conventionally, measurement of a biological substance is performed by collecting blood from a human body and analyzing the blood by a clinical test device. However, blood collection requires several operations to separate necessary components, and there is concern about invasion and infection of the human body.
最近、吸引浸出液の生体関連物質測定への応用が報告
されている（「プロシーディング・オブ・ザ・ファース
ト・パン・パシフィック・シンポジウム、バンクーバ
ー、カナダ」“Proc.of the first Pan Pacific Sympos
ium,Vancouver,Canada",July23−27,1986,57−58.,「プ
ロシーディング・オブ・ザ・シンポジウム・オン・ケミ
カルセンサーズ」“Proc.of the Symposium on Chemica
l Sensors,"PV89−9,327−333.）。吸引浸出液は、角質
層を除去した皮膚の部分を減圧吸引して得られる微量の
液体であり、皮下組織の問質液、あるいは毛細血管壁よ
り減圧濾過され液体であると考えられている。吸引浸出
液は血液よりもタンパク質濃度が低いが、比較的低分子
の生体関連物質については濃度に相関が認められる。こ
のような生体関連物質の測定に関しては、吸引浸出液は
血球部分の分離が必要な血液より適している。また、人
体への感染の点でも、経皮的に採取される吸引浸出液は
有利である。Recently, the application of aspirated leachate to the measurement of biological substances has been reported (“Proceding of the First Pan Pacific Symposium, Vancouver, Canada” “Proc. Of the first Pan Pacific Sympos”).
ium, Vancouver, Canada ", July 23-27, 1986, 57-58." Proceding of the Symposium on Chemical Sensors "," Proc. of the Symposium on Chemica. "
l Sensors, "PV89-9,327-333.) Aspiration exudate is a small amount of liquid obtained by vacuum-suctioning the skin part from which the stratum corneum has been removed. Aspirated leachate has a lower protein concentration than blood, but has a correlation with the concentration of relatively low molecular bio-related substances. The suction leachate is more suitable than blood which requires separation of blood cell parts, and the percutaneous suction leachate is also advantageous in terms of infection to the human body.
第４図に従来の吸引浸出液採取装置の断面図を示し
た。同図において、吸引浸出液採取セル１は、粘着テー
プ２によって皮膚３と密着しており、減圧吸引口４から
の吸引により、皮膚３からスペーサ５を通して吸引浸出
液６を採取するものである。FIG. 4 shows a sectional view of a conventional suction leachate collecting apparatus. In FIG. 1, a suction leachate collection cell 1 is in close contact with the skin 3 by an adhesive tape 2, and collects suction leachate 6 from the skin 3 through a spacer 5 by suction from a reduced-pressure suction port 4.
（発明が解決しようとする課題） 吸引浸出液は微量（0.3u1/cm2）であるので、採取し
た全ての吸引浸出液を回収することが望ましい。しか
し、従来型の採取装置では、内壁に付着するなどして全
ての液を取りだすことができなかった。また、得られた
吸引浸出液の正確な液量を求めることはできなかった。(Problems to be Solved by the Invention) Since the amount of suction leachate is very small (0.3 u1 / cm 2 ), it is desirable to collect all the suction leachate collected. However, with the conventional sampling device, it was not possible to take out all the liquid, for example, by adhering to the inner wall. Further, it was not possible to determine an accurate amount of the obtained suction leachate.
本発明の目的は、上記の課題を解決した吸引浸出液採
取装置、及びこれに基づいた吸引浸出液量測定の方法と
装置、生体関連物質の測定方法と装置を提供することに
ある。An object of the present invention is to provide a suction leachate collecting apparatus which solves the above-mentioned problems, a method and apparatus for measuring the amount of suction leachate based on the apparatus, and a method and apparatus for measuring a biological substance.
（課題を解決するための手段） 本発明は、減圧吸引口の他に、採取した吸引浸出液を
希釈する液の導入口、及び排出口のいずれか、あるいは
両方を備えることを特徴とする吸引浸出液採取装置であ
る。また、希釈された浸出液の正味の液量を知るため
に、希釈液に濃度既知の標準物質を含有させ、これを検
出する部分を排出口に備えた吸引浸出液量測定装置であ
る。吸引浸出液の液量の測定方法は、吸引浸出液量測定
装置の通過した標準物質の濃度を測定し、希釈率を決定
することによって求めることができる。さらに濃度既知
の標準物質を含有させた希釈液を用い、排出口に標準物
質検出部、生体関連物質測定センサを順次備えた生体関
連物質測定装置である。吸引浸出液中の生体関連物質濃
度は、標準物質の濃度を測定して得られた希釈率・浸出
液量によって補正された、生体関連物質測定センサの出
力によって求められる。(Means for Solving the Problems) The present invention provides a suction leachate characterized by comprising, in addition to a reduced pressure suction port, one or both of an introduction port and a discharge port for a liquid for diluting a collected suction leachate. It is a sampling device. Further, in order to know the net liquid amount of the diluted leachate, the suction leachate amount measuring device is provided with a part for detecting the concentration of the standard substance having a known concentration in the diluent at the outlet. The method for measuring the amount of the suction leachate can be determined by measuring the concentration of the standard substance passed through the suction leachate amount measuring device and determining the dilution ratio. Further, the present invention is a biologically relevant substance measuring device that uses a diluent containing a standard substance of a known concentration, and sequentially includes a standard substance detecting unit and a biologically relevant substance measuring sensor at an outlet. The concentration of the biological substance in the suction leachate is obtained from the output of the biological substance measurement sensor corrected by the dilution rate and the amount of the leachate obtained by measuring the concentration of the standard substance.
（作用） 本発明においては、従来の吸引浸出液採取セルに、希
釈液導入と排出口を設け、希釈液を流すことによって、
セル内壁に付着した浸出液をより多く取り出すことがで
きる。緩やかな吸引を短時間行なうことで十分な量の浸
出液を得ることができるので、被験者への負担が少なく
なる。さらに、希釈した浸出液は、水の蒸発による濃縮
効果が小さいので、極微量の浸出液原液に比べ扱い易
い。現在用いられている生体関連物質分析装置やバイオ
センサは、試料を希釈して測定するものが多いので、こ
れらの試料としても適している。(Operation) In the present invention, a diluent introduction and discharge port is provided in a conventional suction leachate collection cell, and the diluent is caused to flow therethrough.
More leachate attached to the inner wall of the cell can be taken out. Since a sufficient amount of exudate can be obtained by performing gentle suction for a short time, the burden on the subject is reduced. Furthermore, since the diluted leachate has a small concentration effect due to the evaporation of water, it is easier to handle than a very small amount of undiluted leachate. Many bio-related substance analyzers and biosensors that are currently used measure a sample by diluting the sample, and are therefore suitable as these samples.
希釈液に濃度既知の標識物質を含有させ、これを検出
し、希釈率を算出することによって、これまで困難であ
った吸引浸出液量の正確な測定が可能となる。さらに、
吸引浸出液の量を測定できるので、生体関連物質の濃度
決定の際の信頼度が向上する。By allowing the diluent to contain a labeled substance of known concentration, detecting this, and calculating the dilution rate, accurate measurement of the amount of suction leachate, which has been difficult until now, becomes possible. further,
Since the amount of the suction leachate can be measured, the reliability in determining the concentration of the biological substance is improved.
（実施例） 本発明の実施例について図面を参照して説明する。(Example) An example of the present invention will be described with reference to the drawings.
第１図は、本発明の一実施例を示す吸引浸出液採取装
置の断面図である。セル１は、粘着テープ２によって皮
膚３と密着しており、スペーサ５を通して減圧吸引口４
より減圧吸引し、浸出液を得る。本実施例ではさらに、
スペーサの端部に希釈液導入口７を、減圧吸引口の下部
に希釈液排出口８を設けている。数分間の吸引によっ
て、スペーサ部分から減圧吸引口の方に吸引浸出液がた
まってきたところで、一定量の希釈液を希釈液導入口よ
り注入し、全量を希釈液排出口より取り出す。FIG. 1 is a sectional view of a suction leachate collecting apparatus showing one embodiment of the present invention. The cell 1 is in close contact with the skin 3 by the adhesive tape 2,
Suction is performed under reduced pressure to obtain a leachate. In this embodiment,
A diluent inlet 7 is provided at the end of the spacer, and a diluent outlet 8 is provided below the reduced-pressure suction port. When the suction leachate accumulates from the spacer portion toward the reduced-pressure suction port by suction for several minutes, a certain amount of diluent is injected from the diluent inlet, and the whole amount is taken out from the diluent outlet.
希釈液を流す際には、減圧吸引口は塞いでおかなけれ
ばならないが、これを希釈液の導入や排出に利用すれ
ば、希釈液導入口と排出口は必ずしも両方備える必要は
ない。When flowing the diluent, the reduced-pressure suction port must be closed, but if this is used for introducing or discharging the diluent, it is not always necessary to provide both the diluent inlet and the outlet.
希釈液の成分としては、人体に無害なものであればよ
く、緩衝溶液や生理食塩水等が適している。また、吸引
浸出液分析のための試薬を混入させておけば、分析操作
が簡略化されてよい。また、吸引部位からの感染を防ぐ
ために殺菌効果のある物質を添加したり、吸引によって
ダメージを受けた部位に対して薬効のある成分を加える
こともできる。As a component of the diluent, any component that is harmless to the human body may be used, and a buffer solution, physiological saline, or the like is suitable. The analysis operation may be simplified by mixing a reagent for analyzing suction leachate. In addition, a substance having a bactericidal effect can be added to prevent infection from the suction site, or a medicinal component can be added to a site damaged by suction.
また、希釈液の排出後、吸引浸出液採取セルの洗浄の
ための液を流し、タンパク質成分の付着を防ぐことによ
って、セルを装着した状態で何度も吸引浸出液を採取す
ることができる。この洗浄液にも、上記の殺菌成分、薬
効成分の添加は有効である。Further, after the diluent is discharged, a liquid for washing the suction leachate collection cell is flowed to prevent the protein component from adhering, whereby the suction leachate can be collected many times with the cell attached. It is effective to add the above-mentioned bactericidal component and medicinal component to this washing liquid.
第２図は、本発明の一実施例を示す吸引浸出液量測定
装置の構成図である。皮膚３に密着したセル１には濃度
既知の標識物質を含有する希釈液９と標識物質検出部10
が結合されている。標識物質検出部では、吸引浸出液に
よって薄められた標識物質の濃度を測定する。FIG. 2 is a configuration diagram of an apparatus for measuring the amount of suction and leachate showing one embodiment of the present invention. The cell 1 in close contact with the skin 3 contains a diluent 9 containing a labeled substance of known concentration and a labeled substance detector 10.
Are combined. The labeling substance detection section measures the concentration of the labeling substance diluted by the suction leachate.
この装置を用いて吸引浸出液の希釈率、及び液量を求
めることができる。その方法を以下に示す。９の希釈液
の堆積をＶ、標識物質の濃度をCm、吸引浸出液の体積を
ｖ、10で検出された標識物質の濃度をCm′とすると、次
の式が成立する。Using this apparatus, the dilution rate and the liquid amount of the suction leachate can be obtained. The method is described below. Assuming that the deposition of the diluent 9 is V, the concentration of the labeling substance is C m , the volume of the suction leachate is v, and the concentration of the labeling substance detected at 10 is C m ′, the following equation is established.
CmV＝Cm′（Ｖ＋ｖ） 希釈度αは、次のように表わされる。C m V = C m ′ (V + v) The degree of dilution α is expressed as follows.
これより、浸出液量ｖは、次のようになる。 Thus, the leaching liquid amount v is as follows.
標識物質としては、検出が容易な物質であるほかに、
人体に無害で他の生体関連物質と反応したり失活させた
りしないものでなければならない。実際に利用可能な系
としては、標識物質として無機塩、類などの電解質を用
い、その伝導度の変化を検出する系、あるいは、標識物
質として紫外や可視領域に吸収をもつ物質を用い、吸光
度の変化をとらえる系、等が挙げられる。 As a labeling substance, in addition to a substance that is easy to detect,
It must be harmless to the human body and not react or inactivate with other biological substances. As a system that can actually be used, a system that uses an electrolyte such as an inorganic salt or the like as a labeling substance and detects a change in its conductivity, or a substance that absorbs in the ultraviolet or visible region as a labeling substance and uses an absorbance System that captures changes in
第３図は、本発明の一実施例を示す生体関連物質測定
装置の構成図である。セル１には濃度既知の標識物質を
含有する希釈液９が導入され、吸引浸出液を含んだ希釈
液は、標識物質検出部10を通って生体関連物質測定用セ
ンサ11に導かれる。標識物質検出部では、吸引浸出液に
よって薄められた標識物質の濃度を測定する。生体関連
物質測定用センサでは、希釈された吸引浸出液に含まれ
る生体関連物質を検出する。センサの出力は標識物質濃
度とともにプロセッサ12に入る。プロセッサでは、セン
サ出力が標識物質の濃度によって補正され、正確な生体
関連物質の濃度が算出される。FIG. 3 is a configuration diagram of a biological-related substance measuring apparatus showing one embodiment of the present invention. A diluent 9 containing a labeling substance of known concentration is introduced into the cell 1, and the diluting liquid containing the suction leachate passes through a labeling substance detection unit 10 and is led to a sensor 11 for measuring a biologically relevant substance. The labeling substance detection section measures the concentration of the labeling substance diluted by the suction leachate. The bio-related substance measuring sensor detects a bio-related substance contained in the diluted suction leachate. The output of the sensor enters the processor 12 along with the label concentration. In the processor, the sensor output is corrected based on the concentration of the labeling substance, and the accurate concentration of the biological substance is calculated.
センサにより得られた、希釈された吸引浸出液中の生
体関連物質の濃度をCb′とすると、吸引浸出液中の値Cb
は、式で求めた希釈度αを用いて次式によって算出
できる。Assuming that the concentration of the biological substance in the diluted suction leachate obtained by the sensor is C b ′, the value C b in the suction leachate is
Can be calculated by the following equation using the dilution degree α obtained by the equation.
この例では、セルからのサンプルが標識物質検出部、
生体関連物質測定用センサの順に導かれているが、この
順序は逆でもよい。 In this example, the sample from the cell is labeled
Although guided in the order of the biomaterial-related sensor, the order may be reversed.
（発明の効果） 以上のように、従来の吸引浸出液採取セルに、希釈液
導入口と排出口を設け、希釈液を流すことによって、セ
ル内壁に付着した浸出液より多く取り出すことができ、
被験者への負担が軽減される。また、標識物質を用いる
ことによって、吸引進出液の液量を測定することが可能
となる。さらに、吸引浸出液量の測定によって、生体関
連物質の測定値の信頼度が向上する。(Effect of the Invention) As described above, the conventional suction leachate collection cell is provided with the diluent introduction port and the discharge port, and by flowing the diluent, it is possible to take out more leachate adhering to the inner wall of the cell,
The burden on the subject is reduced. In addition, by using the labeling substance, it is possible to measure the amount of the suction advance liquid. Furthermore, the reliability of the measured value of the bio-related substance is improved by measuring the suction leachate volume.
【図面の簡単な説明】 第１図は本発明の一実施例を示す吸引浸出液採取装置の
断面図である。第２図は本発明の一実施例を示す吸引浸
出液量測定装置の構成図である。第３図は本発明の一実
施例を示す生体関連物質測定装置の構成図である。第４
図は、従来の吸引浸出液採取装置の断面図である。 図中、１……吸引浸出液採取セル、２……粘着テープ、
３……皮膚、４……減圧吸引口、５……スペーサ、６…
…吸引浸出液、７……希釈液導入口、８……希釈液排出
口、９……標識物質を含む希釈液、10……標識物質検出
部、11……生体関連物質測定用センサ、12……プロセッ
サである。BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a cross-sectional view of a suction leachate collecting apparatus showing one embodiment of the present invention. FIG. 2 is a configuration diagram of an apparatus for measuring the amount of suction and leachate showing one embodiment of the present invention. FIG. 3 is a configuration diagram of a biologically relevant substance measuring device showing one embodiment of the present invention. 4th
The figure is a cross-sectional view of a conventional suction leachate collection device. In the figure, 1 ... suction leachate collection cell, 2 ... adhesive tape,
3 ... skin, 4 ... reduced pressure suction port, 5 ... spacer, 6 ...
... suction leachate, 7 ... diluent introduction port, 8 ... diluent discharge port, 9 ... diluent containing labeling substance, 10 ... labeling substance detection section, 11 ... sensor for measuring biological related substances, 12 ... ... the processor.
Claims (3)
液採取装置において、このセルには採取した吸引浸出液
を希釈する液の導入排出口が１又は２形成されているこ
とを特徴とする吸引浸出液採取装置。1. A suction / leaching liquid collecting apparatus comprising a cell having a reduced-pressure suction port, wherein one or two inlets / outlets of a liquid for diluting the collected suction / leaching liquid are formed in the cell. Leachate collection device.
液採取装置において、標識物質を含有する希釈液の導入
口と、当該希釈液の排出口と、当該排出口に接続する標
識物質検出部を備えたことを特徴とする吸引浸出液量測
定装置。2. A suction leachate collecting apparatus comprising a cell having a reduced pressure suction port, an inlet for a diluting liquid containing a labeling substance, an outlet for the diluting liquid, and a labeling substance detector connected to the discharging port. A suction leachate amount measuring device comprising:
吸引浸出液採取装置において、標識物質を含有する希釈
液の導入口と、当該希釈液の排出口と、当該排出口に接
続する標識物質検出部と、当該検出部に接続する生体関
連物質測定用センサを備えたことを特徴とする生体関連
物質測定装置。3. A suction leachate collecting apparatus having a reduced pressure suction port connected to a suction pump, wherein an inlet for a diluent containing a labeling substance, an outlet for the diluent, and a labeling substance connected to the outlet. A biological-related substance measuring device, comprising: a detecting section; and a biological-related substance measuring sensor connected to the detecting section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2070101A JP2569879B2 (en) | 1990-03-19 | 1990-03-19 | Suction leachate collection device, suction leachate amount measurement device, and biological related substance measurement device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2070101A JP2569879B2 (en) | 1990-03-19 | 1990-03-19 | Suction leachate collection device, suction leachate amount measurement device, and biological related substance measurement device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0428342A JPH0428342A (en) | 1992-01-30 |
| JP2569879B2 true JP2569879B2 (en) | 1997-01-08 |
Family
ID=13421805
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2070101A Expired - Lifetime JP2569879B2 (en) | 1990-03-19 | 1990-03-19 | Suction leachate collection device, suction leachate amount measurement device, and biological related substance measurement device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2569879B2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5483134B2 (en) * | 2012-10-09 | 2014-05-07 | 独立行政法人産業技術総合研究所 | Method and apparatus for measuring volume of microdroplet |
| JP7305382B2 (en) * | 2019-03-15 | 2023-07-10 | 本田技研工業株式会社 | Component concentration measuring device and component concentration measuring method |
-
1990
- 1990-03-19 JP JP2070101A patent/JP2569879B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0428342A (en) | 1992-01-30 |
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