JP2012080839A - Culture system of alga capable of being industrially cultivated - Google Patents

Culture system of alga capable of being industrially cultivated Download PDF

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JP2012080839A
JP2012080839A JP2010230665A JP2010230665A JP2012080839A JP 2012080839 A JP2012080839 A JP 2012080839A JP 2010230665 A JP2010230665 A JP 2010230665A JP 2010230665 A JP2010230665 A JP 2010230665A JP 2012080839 A JP2012080839 A JP 2012080839A
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Chao Hui Lu
朝▲輝▼ 盧
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Abstract

PROBLEM TO BE SOLVED: To provide a culture system of alga which sufficiently increases the yield of quality alga, and can achieve industrial mass culture of alga.SOLUTION: The culture system of alga capable of being industrially cultivated includes: a light transmission pipe of a pipe type photosynthesis unit; a liquid storage unit; a power liquid feeder; a jet-type oxygen discharge unit; and a communicating tube. The liquid storage unit is communicated with an exit part of the light transmission pipe, and is provided with an exhaust port, to which a carbon dioxide gas supply pipe is connected. The power liquid feeder is connected to a terminal of the liquid storage unit, and has a feed liquid tube. The jet-type oxygen discharge unit is formed in a hollow cylindrical body, and has a liquid injection opening, an upper exhaust port, and a hollow tube. The liquid injection opening is communicated with an exit end of the liquid feeding tube. The hollow tube is extended downward of the upper exhaust port. The communication tube has an entrance end communicated with the jet oxygen discharge means, and an exit end connected to the light transmission pipe.

Description

本発明は、工業的に培養可能な藻体の培養システム及びそれを利用した藻体の培養方法に関し、特に、パイプ型培養システムにより藻体の培養液を循環させることで、藻体に光合成を繰り返させる工業的な培養を可能とする藻体の培養システム、及びそれを利用した藻体の培養方法に関するものである。 TECHNICAL FIELD The present invention relates to an industrially cultivatable algal body culture system and an algal body culture method using the same, and in particular, by circulating a culture solution of algal bodies through a pipe type culture system, photosynthesis is performed on the algal bodies. The present invention relates to a culture system for algal bodies that enables repeated industrial culture, and a method for culturing algal bodies using the same.

スピルリナ(Spirulina)やヘマトコッカス(Haematococcus Pluvialis Flotow)、ボトリオコッカス(Botryococcus braunii)などのような藻類は、蛋白質、ミネラル、ビタミン、酵素、酸化防止剤、アスタキサンチンなど人体に有益な栄養素が豊富に含まれており、近年栄養食品として注目されている。 Algae such as Spirulina, Haematococcus Pluvialis Flotow, and Botryococcus braunii are rich in nutrients beneficial to the human body, including proteins, minerals, vitamins, enzymes, antioxidants, and astaxanthin. In recent years, it has attracted attention as a nutritional food.

また、藻類からバイオディーゼル燃料を抽出することが既に可能となっており、代替燃料として利用することも期待されている。 In addition, biodiesel fuel can already be extracted from algae, and it is expected to be used as an alternative fuel.

藻体は光合成反応系により培養液中で光合成を行い、藻細胞が必要としている滋養分を生成し、藍藻の成長と大量繁殖を促進する。 Algae is photosynthetic in the culture solution using a photosynthetic reaction system to generate nutrients required by algal cells, and promotes the growth and mass reproduction of cyanobacteria.

また、光合成で発生した酸素は培養液中に溶解するので、培養液中の溶存酸素濃度を上昇させることができる。 In addition, since oxygen generated by photosynthesis is dissolved in the culture solution, the dissolved oxygen concentration in the culture solution can be increased.

下記特許文献1には、藻類微生物の光合成反応システムと光合成反応方法が開示されている。特許文献1に示される藻類微生物の光合成反応システムでは、藻類は透明なパイプ経路である光合成反応ユニットによって光合成を行い成長する。 Patent Document 1 below discloses a photosynthetic reaction system and a photosynthetic reaction method for algal microorganisms. In the photosynthesis reaction system for algal microorganisms disclosed in Patent Document 1, algae grow by performing photosynthesis using a photosynthesis reaction unit that is a transparent pipe path.

特開2007−319039号公報JP 2007-319039 A

しかしながら、特許文献1に記載されたシステムと方法では、生成される藻体の量が少量である為、工業的な大量生産には適さない。そこで藻体を効率よく生成させることができる方法が求められていた。 However, the system and method described in Patent Document 1 are not suitable for industrial mass production because the amount of alga bodies produced is small. Therefore, a method capable of efficiently generating algal bodies has been demanded.

本発明は、上述した事情に鑑みてなされたものであり、その目的は、藻体を工業的に大量培養することを可能とする藻体の培養システムを提供することにある。
また、本発明のもう一つの目的は、品質の良い藻体を高い産量で培養することを可能とする藻体の培養システムを提供することにある。
This invention is made | formed in view of the situation mentioned above, The objective is to provide the culture system of the alga body which makes it possible to culture an algal body industrially in large quantities.
Another object of the present invention is to provide a culture system for algal cells that makes it possible to culture high-quality algal cells with a high production volume.

前記目的を達成するために、本発明にかかる工業的に培養可能な藻体の培養システムは、パイプ型光合成手段の光透過配管と、液貯留手段と、動力送液手段と、ジェット式酸素排出手段と、連通管手段とを備え、前記液貯留手段は、光透過配管よりも容積が大きく光透過配管の出口部に連通される。また、前記液貯留手段には排気口が設けられるとともに二酸化炭素ガス供給管が接続される。前記動力送液手段は送液管を有し液貯留手段の端末に接続される。前記ジェット式酸素排出手段は、中空筒体に形成され、注液口と、上排気口と、中空管とを有する。注液口は送液管の出口端と連通し、中空管は上排気口の下部に延設される。前記連通管手段は、入口端がジェット酸素排出手段と連通し、出口端が光透過配管と接続される。 In order to achieve the above object, an industrially cultivatable algal cell culture system according to the present invention includes a light transmission pipe of a pipe-type photosynthesis means, a liquid storage means, a power supply means, and a jet oxygen discharge. Means and a communication pipe means, and the liquid storage means has a larger volume than the light transmission pipe and communicates with an outlet portion of the light transmission pipe. The liquid storage means is provided with an exhaust port and connected with a carbon dioxide gas supply pipe. The power supply means has a liquid supply pipe and is connected to a terminal of the liquid storage means. The jet type oxygen discharge means is formed in a hollow cylinder and has a liquid injection port, an upper exhaust port, and a hollow tube. The liquid injection port communicates with the outlet end of the liquid feeding tube, and the hollow tube extends to the lower part of the upper exhaust port. The communication pipe means has an inlet end communicating with the jet oxygen discharging means and an outlet end connected to the light transmission pipe.

好ましくは、光透過配管の上流に複数の連結型密閉容器を備えた細胞分裂手段を更に設ける。 Preferably, a cell division means including a plurality of connected closed containers is further provided upstream of the light transmission pipe.

好ましくは、前記各連結型密閉容器毎に、複数の小型試験管と、一つの中型ビーカーと、一つのエアリフト式光合成器とを備える。前記中型ビーカーは小型試験管の下流に連結され、前記エアリフト式光合成器は前記中型ビーカーの下流に連結され、前記エアリフト式光合成器は、光源手段からの照射を受ける。 Preferably, each of the connected closed containers includes a plurality of small test tubes, one medium beaker, and one airlift photosynthesis device. The medium-sized beaker is connected downstream of a small test tube, the airlift-type light combiner is connected downstream of the medium-sized beaker, and the airlift-type light combiner receives irradiation from light source means.

好ましくは、前記ジェット式酸素排出手
段は、酸素排出筒体と集液部とが構成され、前記酸素排出筒体の側壁には、集液部まで延伸された給気管が設けられる。
Preferably, the jet type oxygen discharge means includes an oxygen discharge cylinder and a liquid collection part, and an air supply pipe extending to the liquid collection part is provided on a side wall of the oxygen discharge cylinder.

好ましくは、前記酸素排出筒体には、排気管が更に設けられ、前記排気管と中空管の上端は第1通気半密閉素子により通気可能に塞がれることで、酸素排出筒体に外部環境からの不純物の進入を防ぐ。また、前記給気管の開口は前記酸素排出筒体側に設けられた第2通気半密閉素子により通気可能に塞がれることで、酸素排出筒体に外部環境からの不純物の進入を防ぐ。 Preferably, the oxygen exhaust cylinder is further provided with an exhaust pipe, and the upper ends of the exhaust pipe and the hollow pipe are closed to be ventilated by a first vent semi-sealing element, so that the oxygen exhaust cylinder is externally provided. Prevent impurities from entering the environment. The opening of the air supply pipe is closed so as to be ventilated by a second ventilation semi-sealing element provided on the oxygen discharge cylinder side, thereby preventing impurities from entering the oxygen discharge cylinder from the outside environment.

好ましくは、前記液貯留手段は配管であり、配管の管径は光透過配管の管径より大径で、底部には採集弁が設けられる。 Preferably, the liquid storage means is a pipe, the pipe diameter is larger than the diameter of the light transmission pipe, and a collecting valve is provided at the bottom.

本発明によると、光透過配管の出口端の後方に液貯留手段が配置され、液貯留手段には光合成により発生した酸素を排出するための排気口が設けられ、更に、光合成で利用されるCOを供給するための二酸化炭素ガス供給管が接続されている。システム内に循環した藻体と培養液は、くり返し光透過配管に入って光合成を行い、液貯留手段内で増殖、成長し続け産量を大幅に増加させることができる。 According to the present invention, the liquid storage means is arranged behind the outlet end of the light transmission pipe, and the liquid storage means is provided with an exhaust port for discharging oxygen generated by photosynthesis, and further used for photosynthesis. A carbon dioxide gas supply pipe for supplying 2 is connected. The algal bodies and the culture solution circulated in the system repeatedly enter the light transmission pipe, perform photosynthesis, continue to grow and grow in the liquid storage means, and can greatly increase production.

藻体を光透過配管に投入する前に、前処理として藻体を細胞分裂手段に入れ、藻体細胞分裂の速度を促進させることで、充分量の藻体細胞数が得られ、予め生物体量が増加された藻体を得ることができる。 Before putting the algal body into the light transmission pipe, as a pretreatment, the algal body is put into a cell division means, and the rate of algal cell division is promoted, so that a sufficient amount of algal cells can be obtained, Algal bodies with increased amounts can be obtained.

生物体量が増加された藻体と培養液は、光透過配管に入れられ、光合成を行い繰り返すことで藻体は生長して増殖し、採集できる藻体になる。 The algal bodies and the culture solution with the increased amount of organisms are put into a light transmission pipe, and by repeating photosynthesis, the algal bodies grow and proliferate and become algal bodies that can be collected.

このように、藻体の細胞分裂と生長増殖の2段階により、藻体の産量を向上させることができる。 Thus, the yield of algal bodies can be improved by two stages of algal cell division and growth.

連結型密閉容器は、小型試験管と、中型ビーカーと、エアリフト式光合成器というサイズがそれぞれ異なった容器を備える。藻体は連結型密閉容器中を流動して光合成を行うことにより、段階的に細胞分裂し、細胞分裂の速度を高めることに寄与する。 The connected sealed containers include containers of different sizes, such as a small test tube, a medium beaker, and an airlift photosynthesis device. Algae body divides cells in stages and contributes to increasing the speed of cell division by flowing through a connected closed container and performing photosynthesis.

更に、気体吹込み装置から二酸化炭素をエアリフト式光合成器に吹き込むことで、培養液に流動が生し、その中の藻体を培養液中に均一に分散させることができ、藻体の成長や細胞分裂を容易にさせ、生物体量を増加させる。 Furthermore, by blowing carbon dioxide from the gas blowing device into the airlift photosynthesis device, a flow is generated in the culture solution, and the algal bodies in the culture solution can be uniformly dispersed in the culture solution. It facilitates cell division and increases the amount of organisms.

また、ジェット式酸素排出手段により給気と排気を行う場合、第1通気半密閉素子と第2通気半密閉素子によりジェット式酸素排出手段を外部からの不純物により汚染されることを防止する。これにより藻体が汚染されることなく生長増殖することができる。 In addition, when air is supplied and exhausted by the jet type oxygen discharge unit, the jet type oxygen discharge unit is prevented from being contaminated by impurities from the outside by the first ventilation semi-sealing element and the second ventilation semi-sealing element. Thereby, it can grow and proliferate without algae being contaminated.

本発明に係る工業的に培養可能な藻体の培養システムを利用して藻体を培養する工程のフローチャートである。It is a flowchart of the process of culture | cultivating an algal body using the culture system of the algal body which can be cultured industrially which concerns on this invention. 本発明の第1実施形態に係る工業的に培養可能な藻体の培養システムを示す模式図であり、液貯留手段2は配管である場合を示す。It is a schematic diagram which shows the culture system of the algal body which can be cultured industrially which concerns on 1st Embodiment of this invention, and shows the case where the liquid storage means 2 is piping. 本発明の第1実施形態に係る工業的に培養可能な藻体の培養システムの変形例を示す模式図であって、液貯留手段2’は開放式の大容量タンクである場合を示す。It is a schematic diagram which shows the modification of the culture system of the alga body which can be cultured industrially which concerns on 1st Embodiment of this invention, Comprising: The liquid storage means 2 'shows the case where it is an open-type large capacity tank. 本発明に係る工業的に培養可能な藻体の培養システムの細胞分裂手段を示す模式図である。It is a schematic diagram which shows the cell division means of the culture system of the algal body which can be cultured industrially based on this invention. 本発明の第2実施形態に係る工業的に培養可能な藻体の培養システムを示す模式図である。It is a schematic diagram which shows the culture system of the alga body which can be cultured industrially which concerns on 2nd Embodiment of this invention. 本発明の第3実施形態に係る工業的に培養可能な藻体の培養システムを示す模式図である。It is a schematic diagram which shows the culture system of the algal body which can be cultured industrially which concerns on 3rd Embodiment of this invention. 本発明の第4実施形態に係る工業的に培養可能な藻体の培養システムを示す模式図である。It is a schematic diagram which shows the culture system of the algal body which can be cultured industrially which concerns on 4th Embodiment of this invention.

以下、本発明の実施形態について、図面を参照しながら説明する。しかしながら本発明の技術的範囲は、これらの実施形態によって限定されるものではなく、発明の要旨を変更することなく様々な形態で実施することができる。 Hereinafter, embodiments of the present invention will be described with reference to the drawings. However, the technical scope of the present invention is not limited by these embodiments, and can be implemented in various forms without changing the gist of the invention.

(第1実施形態)
図1、図2A、図2B及び図3に示すように、本発明に係る工業的に培養可能な藻体の培養システムは、パイプ型光合成手段の光透過配管1と、液貯留手段2と、動力送液手段3と、ジェット式酸素排出手段4と、連通管手段5と、を有する。
(First embodiment)
As shown in FIG. 1, FIG. 2A, FIG. 2B and FIG. 3, an industrially cultivatable algal cell culture system according to the present invention comprises a light transmission pipe 1 of a pipe type photosynthesis means, a liquid storage means 2, Power supply means 3, jet type oxygen discharge means 4, and communication pipe means 5 are provided.

また、本発明に係る藻体の培養システムにおいて、細胞分裂手段6を更に有することが好ましい。 In addition, the algal cell culture system according to the present invention preferably further includes a cell division means 6.

前処理として藻体とその培養液を細胞分裂手段6に投入することで、あらかじめ生物体量(バイオマス)が増加した藻体を生成させることができる。当該生物体量が増加された藻体とその培養液を光透過配管1へ入れ、光合成をさせることで、培養液に光合成後の藻体と酸素が発生する。 By introducing the algal cells and the culture solution into the cell division means 6 as a pretreatment, it is possible to generate algal cells having an increased amount of organisms (biomass) in advance. Algal bodies and oxygen after photosynthesis are generated in the culture solution by putting the algal bodies with the increased amount of organisms and the culture solution into the light transmission pipe 1 and performing photosynthesis.

続いて、光合成後の培養液とその中に含まれる藻体を液貯留手段2へ移送し、さらに動力送液手段3により培養液とその中に含まれる藻体をジェット式酸素排出手段4に移送する。 Subsequently, the culture solution after photosynthesis and the algal bodies contained therein are transferred to the liquid storage means 2, and the culture liquid and the algal bodies contained therein are further transferred to the jet oxygen discharge means 4 by the power feeding means 3. Transport.

ジェット式酸素排出手段4において、培養液中の酸素が排出されると同時に二酸化炭素が培養液に供給され、光合成で消費された二酸化炭素を補充する。 In the jet type oxygen discharge means 4, oxygen in the culture solution is discharged and carbon dioxide is supplied to the culture solution to replenish the carbon dioxide consumed in photosynthesis.

培養液とその中に含まれる藻体は連通管手段5に移送され、そこでしばらくの間生理的調節を行う。 The culture solution and the algal bodies contained therein are transferred to the communication pipe means 5, where physiological adjustment is performed for a while.

その後、光合成後の藻体は、再度光透過配管1へ移送され、前記一連の手順を繰り返す。 Thereafter, the algal bodies after photosynthesis are transferred again to the light transmission pipe 1 and the series of steps is repeated.

このようにくり返し一連の手順を行なうことで、藻体は生長して増殖し、採集できる藻体となる。 By repeating a series of procedures in this way, the alga body grows and proliferates to become an alga body that can be collected.

最後に、藻体は再び液貯留手段2に流れ込んで貯留され、そこで生長して大量に増殖し続け、採集可能となる。 Finally, the algal bodies again flow into the liquid storage means 2 and are stored therein, where they grow and continue to grow in large quantities and can be collected.

光透過配管1の周囲に補光照射手段11と噴水手段12を配置してもよい。また、光透過配管1の上流側に注入口101を設けると、藻体や培養液を容易に光透過配管1に注入することができるので好ましい。光透過配管1は上流側から下流側に向かって延長方向に螺旋状に配置され、注入口101から注入された藻体や培養液は、螺旋を描くように光透過配管1を流れる。 The supplementary light irradiation means 11 and the fountain means 12 may be disposed around the light transmission pipe 1. In addition, it is preferable to provide the inlet 101 on the upstream side of the light transmission pipe 1 because alga bodies and culture solution can be easily injected into the light transmission pipe 1. The light transmission pipe 1 is spirally arranged in the extending direction from the upstream side to the downstream side, and the algal bodies and the culture solution injected from the injection port 101 flow through the light transmission pipe 1 so as to draw a spiral.

補光照射手段11は蛍光灯具であってもよく、LEDランプであってもよい。補光照射手段11から出射された光を光透過配管1に照射することで、光合成に必要な十分な光強度が得られる。補光照射手段11が設けられる位置は特に限定されず、光透過配管1が受ける光の強さを藻体の光合成に必要な光強度にするように補光照射手段11を光透過配管1の付近に適宜設ければよい。なお、光合成に必要な光強度は、藻体の種類により異なるので、補光照射手段11の光線出射角度や光強度は必要に応じて変更する。 The supplementary light irradiation means 11 may be a fluorescent lamp or an LED lamp. By irradiating the light transmission pipe 1 with the light emitted from the supplementary light irradiation means 11, sufficient light intensity required for photosynthesis can be obtained. The position where the supplementary light irradiating means 11 is provided is not particularly limited. What is necessary is just to provide suitably in the vicinity. In addition, since the light intensity required for photosynthesis changes with kinds of algae, the light beam emission angle and light intensity of the supplementary light irradiation means 11 are changed as needed.

噴水手段12は、手動操作式のものであってもよく、誘導型のものであってもよい。噴水手段12から噴き出す水を光透過配管1に当てることで、光透過配管1内の培養液の温度を低下させ、補光照射手段11による熱から守ることができる。 The fountain means 12 may be manually operated or guided. By applying water sprayed from the fountain means 12 to the light transmission pipe 1, the temperature of the culture solution in the light transmission pipe 1 can be lowered and protected from the heat by the supplementary light irradiation means 11.

本実施形態において、噴水手段12は光透過配管1の上方に配置されているが、噴水手段12が設けられる位置は特に限定されず、光透過配管1の温度を藻体の光合成に必要な温度にするように光透過配管1の付近に適宜設ければよい。 In the present embodiment, the fountain means 12 is disposed above the light transmission pipe 1, but the position where the fountain means 12 is provided is not particularly limited, and the temperature of the light transmission pipe 1 is a temperature necessary for the photosynthesis of the algal bodies. What is necessary is just to provide suitably in the vicinity of the light transmission piping 1 so that it may become.

なお、光合成に必要な温度は、藻体の種類により異なるので、必要に応じて、噴水手段12により培養液の温度を調整する。 In addition, since the temperature required for photosynthesis changes with the kind of alga bodies, the temperature of a culture solution is adjusted with the fountain means 12 as needed.

液貯留手段2は、光透過配管1よりも容積が大きく光透過配管1の出口部に連通される。液貯留手段2には、排気口21が設けられるとともに、二酸化炭素ガス供給管22が接続される。 The liquid storage means 2 has a larger volume than the light transmission pipe 1 and communicates with the outlet of the light transmission pipe 1. The liquid storage means 2 is provided with an exhaust port 21 and connected with a carbon dioxide gas supply pipe 22.

光合成により発生した酸素は、排気口21から外部へ放出されると共に、光合成に必要なCOが二酸化炭素ガス供給管22から培養液中に補充される。これにより、藻体は液貯留手段2内に増殖、生長し続けることができる。 Oxygen generated by the photosynthesis is released from the exhaust port 21 to the outside, and CO 2 necessary for photosynthesis is replenished into the culture solution from the carbon dioxide gas supply pipe 22. Thereby, algal bodies can continue to grow and grow in the liquid storage means 2.

液貯留手段2は図2Aに示すように配管にしてもよく、また、図2Bに示すように、液貯留手段2’を開放式の大容量タンクにしてもよい。 The liquid storage means 2 may be a pipe as shown in FIG. 2A, and the liquid storage means 2 'may be an open large-capacity tank as shown in FIG. 2B.

開放式タンクの場合、不純物が外部から培養液中に入ることを防ぐため、タンクの上に液貯留槽の開口を覆うための蓋部などが設けられる。蓋部以外の部分については液貯留手段2と同様に排気口21’と二酸化炭素ガス供給管22’が設けられる。 In the case of an open tank, a lid for covering the opening of the liquid storage tank is provided on the tank in order to prevent impurities from entering the culture solution from the outside. As for the portion other than the lid, an exhaust port 21 ′ and a carbon dioxide gas supply pipe 22 ′ are provided in the same manner as the liquid storage means 2.

液貯留手段2は密閉性と藻体の不純物汚染防止など衛生上の観点から、配管の方がより好ましい。その場合管体をU字状にし地上に設ける。その際、配管の管径は光透過配管1の管径より大径にする必要がある。また、配管である液貯留手段2の底部に藻体を採集するための採集弁23を設けてもよい。 The liquid storage means 2 is more preferably a pipe from the viewpoint of hygiene such as hermeticity and prevention of impurity contamination of algal bodies. In that case, the tube body is made U-shaped and provided on the ground. At that time, the pipe diameter needs to be larger than the pipe diameter of the light transmission pipe 1. Moreover, you may provide the collection valve 23 for collecting an algal body in the bottom part of the liquid storage means 2 which is piping.

培養液は光透過配管1中での流速が早いので、受けた光量が増し、藻体の成長速度も迅速である。一方、液貯留手段2に流れ込むと、流速が緩やかになるために、藻体が凝集して凝集物を形成する。凝集された藻体は、液貯留手段2において細胞分裂を行って大量に増殖する。 Since the culture fluid has a high flow rate in the light transmission pipe 1, the amount of light received increases and the growth rate of the algal bodies is also rapid. On the other hand, when it flows into the liquid storage means 2, the flow rate becomes gentle, and the algal bodies aggregate to form aggregates. Aggregated alga bodies undergo cell division in the liquid storage means 2 and grow in large quantities.

図2A、図2Bに示すように、液貯留手段2の端末には送液管31と加圧送液ポンプ32とを有する動力送液手段3が接続される。送液管31は、入口端が液貯留手段2に接続され、出口端が下記ジェット式酸素排出手段4に接続される。送液管31中の培養液は、加圧送液ポンプ32により加圧されて、ジェット式酸素排出手段4に移送される。 As shown in FIGS. 2A and 2B, the power supply means 3 having a liquid supply pipe 31 and a pressurized liquid supply pump 32 is connected to the terminal of the liquid storage means 2. The liquid feed pipe 31 has an inlet end connected to the liquid storage means 2 and an outlet end connected to the jet oxygen discharge means 4 described below. The culture solution in the liquid feeding tube 31 is pressurized by the pressurized liquid feeding pump 32 and transferred to the jet oxygen discharge means 4.

ジェット式酸素排出手段4は、互いに取り付けられた酸素排出筒体41と集液部42とから構成される。中空筒体を成し、注液口411、上排気口412、及び中空管413を備える。注液口411は、送液管31の出口端と連通される。上排気口412は酸素排出筒体41の上部に開口し、上排気口412の下部には中空管413が延設される。また、酸素排出筒体41には、排気管43を更に設けてもよい。当該排気管43には下端に向けて徐々に内径を拡げたテーパー状の拡大部431が形成される。また、酸素排出筒体41の側壁には、集液部42まで延伸された給気管44を設けてもよい。 The jet type oxygen discharge means 4 includes an oxygen discharge cylinder 41 and a liquid collecting part 42 attached to each other. A hollow cylinder is formed, and a liquid injection port 411, an upper exhaust port 412, and a hollow tube 413 are provided. The liquid injection port 411 communicates with the outlet end of the liquid feeding pipe 31. The upper exhaust port 412 opens to the upper part of the oxygen exhaust cylinder 41, and a hollow tube 413 extends from the lower part of the upper exhaust port 412. The oxygen exhaust cylinder 41 may further be provided with an exhaust pipe 43. The exhaust pipe 43 is formed with a tapered enlarged portion 431 whose inner diameter is gradually increased toward the lower end. Further, an air supply pipe 44 extending to the liquid collecting part 42 may be provided on the side wall of the oxygen discharge cylinder 41.

上述の構成により動力送液手段3により移送された培養液は、注液口411から集液部42に流入する際、ジェット状に噴出し中空管413に衝突する。これにより培養液を回旋して酸素ガスを上排気口412から排出させる。 When the culture liquid transferred by the power feeding means 3 with the above-described configuration flows into the liquid collection part 42 from the liquid injection port 411, it is jetted out and collides with the hollow tube 413. As a result, the culture solution is rotated to discharge oxygen gas from the upper exhaust port 412.

また、培養液が落下する際、拡大部431に衝突し、培養液を飛散させ、酸素ガスの排出を更に促進する。その後、培養液は集液部42の底部に集まって沈む。なお、集液部42の底部の培養液へ、藻体の光合成に必要な二酸化炭素を給気管44から供給すると、培養液における藻体に再び光合成を行う能力を高めることができる。このように、ジェット式酸素排出手段4の構成により、上排気口412から培養液の酸素が排出され、給気管44から二酸化炭素が補充される。 In addition, when the culture solution falls, it collides with the enlarged portion 431, scatters the culture solution, and further promotes the discharge of oxygen gas. Thereafter, the culture solution collects and sinks at the bottom of the liquid collection part 42. In addition, if the carbon dioxide required for the photosynthesis of the algal cells is supplied from the supply pipe 44 to the culture solution at the bottom of the liquid collecting unit 42, the ability to perform photosynthesis again on the algal cells in the culture solution can be enhanced. As described above, due to the configuration of the jet type oxygen discharge means 4, oxygen of the culture solution is discharged from the upper exhaust port 412 and carbon dioxide is replenished from the supply pipe 44.

ジェット式酸素排出手段4には、第1通気半密閉素子45と第2通気半密閉素子46を更に設けることが好ましい。排気管43と中空管413の上端の開口を第1通気半密閉素子45で通気可能に塞ぐことで、ジェット式酸素排出手段4内に外部からの不純物の進入を遮断することができる。 The jet type oxygen discharge means 4 is preferably further provided with a first ventilation semi-sealing element 45 and a second ventilation semi-sealing element 46. By closing the openings at the upper ends of the exhaust pipe 43 and the hollow pipe 413 with the first ventilation semi-sealing element 45, it is possible to block the entry of impurities from the outside into the jet type oxygen discharge means 4.

同様に酸素排出筒体41の側壁に設けられた前記給気管44の開口を前記酸素排出筒体41側に設けられた第2通気半密閉素子46で通気可能に塞ぐことで、ジェット式酸素排出手段4内に外部からの不純物の進入を遮断することができる。 Similarly, the opening of the air supply pipe 44 provided on the side wall of the oxygen discharge cylinder 41 is closed so as to be ventilated by the second ventilation semi-sealing element 46 provided on the oxygen discharge cylinder 41 side, thereby jet-type oxygen discharge. It is possible to block the entry of impurities from the outside into the means 4.

また、第1通気半密閉素子45と第2通気半密閉素子46には、フィルターやエアバルブを用いると、酸素や二酸化炭素などのガスが通過可能となると共にジェット式酸素排出手段4を外部環境の不純物から守ることができる。 Further, if a filter or an air valve is used for the first ventilation semi-sealing element 45 and the second ventilation semi-sealing element 46, a gas such as oxygen or carbon dioxide can pass through and the jet type oxygen discharge means 4 can be connected to the external environment. Can be protected from impurities.

連通管手段5は入口端がジェット酸素排出手段4と連通し、出口端が光透過配管1と接続される。培養液はジェット式酸素排出手段4から連通管手段5に流入し、更に光透過配管1内に流入することで、培養液における藻体は再度光合成を行い、増殖することができる。 The communicating pipe means 5 has an inlet end communicating with the jet oxygen discharging means 4 and an outlet end connected to the light transmission pipe 1. The culture fluid flows from the jet type oxygen discharge means 4 into the communication pipe means 5 and then into the light transmission pipe 1 so that the algal bodies in the culture liquid can again undergo photosynthesis and proliferate.

図3に示すように、光透過配管1の上流には細胞分裂手段6が設けられる。前処理として藻体を細胞分裂手段6に入れ、藻体細胞分裂の速度を促進させることで、充分量の藻体細胞数が得られ、生物体量が増加された藻体を得ることができる。 As shown in FIG. 3, cell division means 6 is provided upstream of the light transmission pipe 1. As a pretreatment, an algal body is put into the cell division means 6 and the rate of algal cell division is promoted, whereby a sufficient number of algal cells can be obtained and an algal body with an increased amount of organisms can be obtained. .

細胞分裂手段6は、複数の連結型密閉容器60を有する。また、各連結型密閉容器60毎には、複数の小型試験管61と、一つの中型ビーカー62と、一つのエアリフト式光合成器63とが備えられる。小型試験管61の下流には中型ビーカー62が連結され、中型ビーカー62の下流にはエアリフト式光合成器63が連結される。このように、連続して密閉的な連結型密閉容器60を構成する。 The cell division means 6 has a plurality of connected closed containers 60. Each of the connected sealed containers 60 is provided with a plurality of small test tubes 61, one medium beaker 62, and one air lift type photocombiner 63. A medium-sized beaker 62 is connected downstream of the small test tube 61, and an airlift photosynthesis device 63 is connected downstream of the medium-sized beaker 62. In this way, the continuous connected sealed container 60 that is hermetically sealed is configured.

各小型試験管61毎に藻体と培養液を入れ、藻体を細胞分裂させる。藻体が細胞分裂して所定量の細胞数となった後、各小型試験管61の藻体と培養液を中型ビーカー62に入れ、再び藻体を細胞分裂させる。最後に、中型ビーカー62の藻体と培養液をエアリフト式光合成器63に入れる。藻体はここで光源手段64からの照射を受け光合成を行う。このように、藻体は各連結型密閉容器60中に細胞分裂の速度が促進され、充分量の藻体細胞数を得ることができる。 For each small test tube 61, the algal cells and the culture solution are added to divide the algal cells. After the algal cells have divided into a predetermined number of cells, the algal cells and the culture solution of each small test tube 61 are put into the medium beaker 62, and the algal cells are again divided. Finally, the algae bodies and the culture solution of the medium beaker 62 are put into the airlift photosynthesis device 63. The algal body is irradiated with light from the light source means 64 and performs photosynthesis. As described above, the algal cells are accelerated in cell division in each of the connected closed containers 60, and a sufficient number of algal cells can be obtained.

各エアリフト式光合成器63には各々一つの光源手段64が装備される。光源手段64は、第1光源641と、第2光源642と、電源643と電気制御装置644を備える。第1光源641と第2光源642は電気制御装置644を介して電源643に電気的に接続され、点灯に必要な電気の供給を受ける。 Each air lift type photocombiner 63 is provided with one light source means 64. The light source means 64 includes a first light source 641, a second light source 642, a power source 643, and an electric control device 644. The first light source 641 and the second light source 642 are electrically connected to the power source 643 via the electric control device 644 and receive supply of electricity necessary for lighting.

また、電気制御装置644により第1光源641と第2光源642の輝度を調整することで、エアリフト式光合成器63内の藻体や培養液の温度を制御することができる。 Further, by adjusting the luminance of the first light source 641 and the second light source 642 by the electric control device 644, the temperature of the algal bodies and the culture solution in the airlift photosynthesis device 63 can be controlled.

第1光源641はエアリフト式光合成器63の外部に設けられ、第2光源642はエアリフト式光合成器63の内部に設けられる。これにより、藻体に光線が充分に照射し、藻体は光合成により、生物体量を増加することができる。 The first light source 641 is provided outside the air lift type light combiner 63, and the second light source 642 is provided inside the air lift type light combiner 63. Thereby, a light beam is sufficiently irradiated to the alga body, and the alga body can increase the amount of living organisms by photosynthesis.

なお、連結型密閉容器60には、気体吹込み装置65を更に備えてもよい。気体吹込み装置65は、第1配管651と、第2配管652と、第3配管653とを有し、第1配管651は各小型試験管61に連接され、第2配管652は各中型ビーカー62に連接され、第3配管653は各エアリフト式光合成器63に連接される。 The connected airtight container 60 may further include a gas blowing device 65. The gas blowing device 65 includes a first pipe 651, a second pipe 652, and a third pipe 653. The first pipe 651 is connected to each small test tube 61, and the second pipe 652 is each medium-sized beaker. 62 and the third pipe 653 is connected to each of the air lift type optical combiners 63.

気体吹込み装置65で発生させた二酸化炭素を第1配管651、第2配管652と第3配管653を介してそれぞれ小型試験管61、中型ビーカー62とエアリフト式光合成器63に吹き込むことで、培養液に流動が生じ、培養液中の藻体を均一に分散させる。これにより藻体の成長や細胞分裂を容易にさせ、生物体量が増加された藻体を得ることができる。 The carbon dioxide generated by the gas blowing device 65 is blown into the small test tube 61, the medium beaker 62, and the airlift photosynthesis device 63 through the first pipe 651, the second pipe 652, and the third pipe 653, respectively, thereby culturing. Flow occurs in the liquid, and the algal bodies in the culture liquid are uniformly dispersed. Thereby, the growth and cell division of algal bodies are facilitated, and the algal bodies having an increased amount of organisms can be obtained.

光合成により発生した酸素は放気管631から外部へ放出される。一方、生物体量が増加した藻体と培養液は、合流配管66中を流れて、注入口101から光透過配管1に流入し、光合成を行い続ける。 Oxygen generated by photosynthesis is released to the outside from the air discharge pipe 631. On the other hand, the algal bodies and the culture solution with the increased amount of organisms flow through the merging pipe 66 and flow into the light transmission pipe 1 from the injection port 101 and continue photosynthesis.

上記のように、接続されて密閉的な連結型密閉容器60の環境は、藻体の細胞分裂に寄与し、培養液中に生物体量を増加させた藻体が得られ、藻体の産量を向上させることができる。 As described above, the environment of the connected sealed container 60 that is connected and sealed contributes to cell division of the alga body, and an algal body with an increased amount of organisms is obtained in the culture solution. Can be improved.

(第2実施形態)
また、本発明に係る実施形態の変形例として、ジェット式酸素排出手段と連通管手段を設計することができる。
(Second Embodiment)
Further, as a modification of the embodiment according to the present invention, a jet type oxygen discharge means and a communication pipe means can be designed.

図4は、本発明の第2実施形態に係る工業的に培養可能な藻体の培養システムを示す模式図である。第2実施形態に係る培養システムにおけるジェット式酸素排出手段4には本体の長さを比較的長めにした集液部42aを使用し、ジェット式酸素排出手段4と連通管手段5aとを並列に並べて構成する点で前記第1実施形態と相違する。 FIG. 4 is a schematic diagram showing an industrially cultivated algal culture system according to a second embodiment of the present invention. The jet type oxygen discharge means 4 in the culture system according to the second embodiment uses a liquid collection part 42a having a relatively long main body, and the jet type oxygen discharge means 4 and the communication pipe means 5a are arranged in parallel. It differs from the first embodiment in that it is arranged side by side.

本実施形態における連通管手段5aは連通入水口51aと、拡大式連通管52aと、連通出水管53aとを備える。拡大式連通管52aの、底部は連通入水口51aに連結され、上部は連通出水管53aに連結される。連通入水口51aは集液部42と連通される。連通出水管53aは光透過配管1の最上部の入口に連結される。 The communication pipe means 5a in the present embodiment includes a communication water inlet 51a, an expansion communication pipe 52a, and a communication water discharge pipe 53a. The bottom of the expansion communication pipe 52a is connected to the communication inlet 51a, and the upper part is connected to the communication outlet 53a. The communication inlet 51 a communicates with the liquid collection part 42. The communication outlet pipe 53 a is connected to the uppermost inlet of the light transmission pipe 1.

これにより、集液部42に溜まった培養液の液面高度が拡大式連通管52aの液面高度より高くなると、圧力により培養液が連通出水管53aから光透過配管1へ流動し、光透過配管1に入る藻体は再度光合成を行い生長、増殖する。 As a result, when the liquid level of the culture liquid accumulated in the liquid collecting part 42 becomes higher than the liquid level of the expansion communication pipe 52a, the culture liquid flows from the communication outlet pipe 53a to the light transmission pipe 1 due to the pressure, and the light is transmitted. Algae entering the pipe 1 is again photo-synthesized and grows and grows.

本実施形態では長さを長めにした集液部42aと大径の拡大式連通管52aを用いることにより、培養液の流速を緩徐させ、培養液が連通管手段5aから光透過配管1に流入する時間を長くさせることにより、藻体が生理的調節を行う充分な時間があり、これにより動力送液手段3やジェット式酸素排出手段4による生理的障害を解消することができ、品質の良い藻体を得ることができる。 In this embodiment, the flow rate of the culture solution is slowed by using the liquid collecting part 42a having a long length and the large-diameter enlarged communication pipe 52a, and the culture liquid flows into the light transmission pipe 1 from the communication pipe means 5a. By extending the time to perform, there is sufficient time for the algal bodies to perform physiological regulation, thereby eliminating the physiological obstacles caused by the power feeding means 3 and the jet type oxygen discharging means 4, and the quality is good. Algae can be obtained.

(第3実施形態)
図5は、本発明の第3実施形態に係る工業的に培養可能な藻体の培養システムを示す模式図である。
(Third embodiment)
FIG. 5 is a schematic diagram showing an industrially cultivatable algal cell culture system according to a third embodiment of the present invention.

連通管手段5bの数量は必要に応じて変更することができる。例えば、二つまたはそれ以上の連通管手段5bを互いに流通させるように連結することで、互いに流通させた連続式の連通管手段5bを構成してもよい。つまり、複数本並べて配置された拡大式連通管52bにより、連続式の拡大式連通管52bが構成され、且つ連続式の拡大式連通管52bはジェット式酸素排出手段4と光透過配管1と連通される。 The quantity of the communication pipe means 5b can be changed as necessary. For example, the continuous communication pipe means 5b circulated to each other may be configured by connecting two or more communication pipe means 5b so as to circulate each other. That is, a continuous expansion communication pipe 52b is constituted by a plurality of expansion communication pipes 52b arranged side by side, and the continuous expansion communication pipe 52b communicates with the jet oxygen discharge means 4 and the light transmission pipe 1. Is done.

2個で一組の連続式の拡大式連通管52bの下部には、連続式の拡大式連通管52b同士を連結させる開閉弁部材71が更に設けられている。 An opening / closing valve member 71 for connecting the continuous expansion communication pipes 52b to each other is further provided below the two sets of continuous expansion communication pipes 52b.

各拡大式連通管52bごとには気体経路72が接続されている。更に、2個で一組の連続式の拡大式連通管52bの上部に接続された配管は、酸素の排出と不純物の侵入防止のために、先端を下方に湾曲させ、下方に向いて開口した酸素排出口54bが形成されている。 A gas path 72 is connected to each expansion communication pipe 52b. Further, the pipes connected to the upper part of the two sets of continuous expansion communication pipes 52b are curved downward at the tips and opened downward to prevent oxygen discharge and impurities from entering. An oxygen discharge port 54b is formed.

気体経路72を設ける位置は、藻体の流動方向に応じて設けることが好ましく、例えば、藻体が下部から流入する場合、拡大式連通管52bの下部に設け、上部から流入する場合には拡大式連通管52bの上部に設ければよい。 The position where the gas path 72 is provided is preferably provided according to the flow direction of the algal bodies. For example, when the algal bodies flow from the lower part, they are provided at the lower part of the expansion communication pipe 52b. What is necessary is just to provide in the upper part of the type | formula communication pipe | tube 52b.

このような連続式の拡大式連通管52bにより、ジェット式酸素排出手段4から導入された藻体は連続的に生理的調節を行うことができる。 With such a continuous expansion communication pipe 52b, the algal bodies introduced from the jet oxygen discharge means 4 can be continuously physiologically adjusted.

また、このように互いに流通させた連続式の連通管手段5bに、気体経路72から二酸化炭素ガスを吹き込むことで、培養液に流動が生じ、培養液中の藻体を均一に分散させる。これにより藻体の生長や細胞分裂を促進し、生物体量を増加させることができる。 Further, by blowing carbon dioxide gas from the gas path 72 into the continuous communication pipe means 5b circulated to each other in this way, a flow is generated in the culture solution, and the algal bodies in the culture solution are uniformly dispersed. Thereby, the growth of algal bodies and cell division can be promoted, and the amount of organisms can be increased.

システム全体を循環して成長を完了した藻体は、開閉弁部材71により排出され採集することができる。 The algal bodies that have completed the growth through circulation of the entire system can be discharged and collected by the on-off valve member 71.

本実施形態に係る連続式の連通管手段5bを前記実施形態のものと比較すると、システムの稼動を止めることなく一日1回または二日に1回くらいのペースで採集することができる効果が期待され、次の洗浄を行うまでに大量且つ継続的に採集することができる。 When the continuous communication pipe means 5b according to the present embodiment is compared with that of the above-described embodiment, there is an effect that the collection can be performed once a day or once every two days without stopping the operation of the system. Expected and can be collected continuously in large quantities before the next wash.

(第4実施形態)
工業的に藻体を大量生産する目的を達成するために、光透過配管1の管径を大きくし、長さを長くすることにより産量を増加させるのは一般的であるが、配管中の酸素含有量が増加され、藻体の成長を抑える結果を招くことがある。このような課題を解決するために、図6に示す本発明の第4実施形態では、光透過配管1の中間部に排気するための通気弁部材73が設けられている。通気弁部材73は、光透過配管1に連通される連通水管731と、連通水管731に連通されるとともに上端の高さを光透過配管1より高くした排気管732と、連通水管731を開閉するための開閉弁733と、排出弁734とを有する。開閉弁733が開かれると、培養液は排気管732に流入するが、排気管732は上端の高さが光透過配管1より高いので、培養液は排気管732から溢流することがない。一方、酸素は排気管732から外部に排出することができる。また、酸素の外部への放出を容易にするための手段を設けてもよい。更に、排出弁734が開いている際、老廃した藻体や壊死した藻体を排出することができる。
(Fourth embodiment)
In order to achieve the purpose of industrially mass-producing algal bodies, it is common to increase the production volume by increasing the pipe diameter of the light transmission pipe 1 and increasing the length, but oxygen in the pipe The content is increased, which may result in suppressing the growth of algal bodies. In order to solve such a problem, in the fourth embodiment of the present invention shown in FIG. 6, a vent valve member 73 for exhausting air to the intermediate part of the light transmission pipe 1 is provided. The vent valve member 73 opens and closes the communication water pipe 731 that communicates with the light transmission pipe 1, the exhaust pipe 732 that communicates with the communication water pipe 731 and has an upper end higher than the light transmission pipe 1, and the communication water pipe 731. And an on-off valve 733 and a discharge valve 734. When the on-off valve 733 is opened, the culture solution flows into the exhaust pipe 732, but the upper end of the exhaust pipe 732 is higher than the light transmission pipe 1, so that the culture solution does not overflow from the exhaust pipe 732. On the other hand, oxygen can be discharged from the exhaust pipe 732 to the outside. Further, means for facilitating the release of oxygen to the outside may be provided. Furthermore, when the discharge valve 734 is open, the aged and necrotic algae can be discharged.

本発明に係る工業的に培養可能な藻体の培養システムによると、その構造設計により大幅に藻体の収量を向上させ、工業的な大量生産に適する藻体の培養システムを提供することができる。 According to the industrially cultivatable algal cell culture system according to the present invention, the yield of algal cells can be greatly improved by the structural design, and an algal culture system suitable for industrial mass production can be provided. .

1 光透過配管
101 注入口
11 補光照射手段
12 噴水手段
2 液貯留手段
21 排気口
22 二酸化炭素ガス供給管
23 採集弁
2’ 液貯留手段
21’ 排気口
22’ 二酸化炭素ガス供給管
3 動力送液手段
31 送液管
32 加圧送液ポンプ
4 ジェット式酸素排出手段
41 酸素排出筒体
411 注液口
412 上排気口
413 中空管
42,42a 集液部
43 排気管
431 拡大部
44 給気管
45 第1通気半密閉素子
46 第2通気半密閉素子
5 連通管手段
5a 連通管手段
51a 連通入水口
52a 拡大式連通管
53a 連通出水管
5b 連通管手段
52b 拡大式連通管
54b 酸素排出口
6 細胞分裂手段
60 連結型密閉容器
61 小型試験管
62 中型ビーカー
63 エアリフト式光合成器
631 放気管
64 光源手段
641 第1光源
642 第2光源
643 電源
644 電気制御装置
65 気体吹込み装置
651 第1配管
652 第2配管
653 第3配管
66 合流配管
71 開閉弁部材
72 気体経路
73 通気弁部材
731 連通水管
732 排気管
733 開閉弁
734 排出弁
DESCRIPTION OF SYMBOLS 1 Light transmission piping 101 Inlet 11 Supplementary light irradiation means 12 Fountain means 2 Liquid storage means 21 Exhaust port 22 Carbon dioxide gas supply pipe 23 Collection valve 2 'Liquid storage means 21' Exhaust port 22 'Carbon dioxide gas supply pipe 3 Power transmission Liquid means 31 Liquid feed pipe 32 Pressurized liquid feed pump 4 Jet type oxygen discharge means 41 Oxygen discharge cylinder 411 Injection port 412 Upper exhaust port 413 Hollow pipes 42 and 42a Liquid collection part 43 Exhaust pipe 431 Enlarged part 44 Air supply pipe 45 First vent semi-sealing element 46 Second vent semi-sealing element 5 Communication pipe means 5a Communication pipe means 51a Communication inlet 52a Expandable communication pipe 53a Communication outlet pipe 5b Communication pipe means 52b Expandable communication pipe 54b Oxygen outlet 6 Cell division Means 60 Connected closed vessel 61 Small test tube 62 Medium beaker 63 Air lift type photocombiner 631 Air discharge pipe 64 Light source means 641 First light source 642 Second light source 643 Power supply 644 Electric control device 65 Gas blowing device 651 First pipe 652 Second pipe 653 Third pipe 66 Merge pipe 71 Open / close valve member 72 Gas path 73 Vent valve member 731 Communication water pipe 732 Exhaust pipe 733 Open / close valve 734 Discharge valve

Claims (10)

工業的に培養可能な藻体の培養システムであって、
パイプ型光合成手段の光透過配管と、
前記光透過配管の出口部に連通される液貯留手段であって、前記光透過配管よりも容積が大きく、排気口が設けられるとともに二酸化炭素ガス供給管が接続される液貯留手段と、
送液管を有し、前記液貯留手段の端末に接続される動力送液手段と、中空筒体に形成されるジェット式酸素排出手段であって、注液口と、上排気口と、中空管とを有し、前記注液口は前記送液管の出口端と連通し、前記中空管は前記上排気口の下部に延設されるジェット式酸素排出手段と、
入口端が前記ジェット酸素排出手段と連通するとともに出口端が前記光透過配管と接続される連通管手段と、
を備えることを特徴とする工業的に培養可能な藻体の培養システム。
An industrially cultivated algal culture system,
A light transmission pipe of a pipe-type photosynthesis means;
Liquid storage means communicated with an outlet portion of the light transmission pipe, the liquid storage means having a larger volume than the light transmission pipe, provided with an exhaust port, and connected with a carbon dioxide gas supply pipe;
A power supply means having a liquid supply pipe and connected to a terminal of the liquid storage means; a jet type oxygen discharge means formed in a hollow cylindrical body; a liquid injection port; an upper exhaust port; A jet-type oxygen discharge means that extends from the upper exhaust port to the lower end of the upper exhaust port, and the liquid injection port communicates with an outlet end of the liquid supply tube.
A communication pipe means having an inlet end communicating with the jet oxygen discharging means and an outlet end connected to the light transmission pipe;
A culture system for industrially cultivated alga bodies, comprising:
更に、前記光透過配管の上流に細胞分裂手段が設けられ、前記細胞分裂手段は、複数の連結型密閉容器を備えることを特徴とする、請求項1に記載の工業的に培養可能な藻体の培養システム。 The industrially cultivatable alga according to claim 1, further comprising a cell division means provided upstream of the light transmission pipe, wherein the cell division means comprises a plurality of connected closed containers. Culture system. 前記各連結型密閉容器は、複数の小型試験管と、一つの中型ビーカーと、一つのエアリフト式光合成器とを備え、前記中型ビーカーは前記小型試験管の下流に連結され、前記エアリフト式光合成器は前記中型ビーカーの下流に連結され、前記エアリフト式光合成器は、光源手段からの照射を受けることを特徴とする、請求項2に記載の工業的に培養可能な藻体の培養システム。 Each of the connected closed containers includes a plurality of small test tubes, one medium beaker, and one airlift photosynthesis device, and the medium beaker is connected downstream of the small test tubes, and the airlift photosynthesis device. The apparatus for cultivating alga bodies according to claim 2, wherein the cultivated alga body is connected to a downstream side of the medium-sized beaker, and the airlift photosynthesizer receives irradiation from a light source means. 前記光源手段は、第1光源と、第2光源と、電源とを備え、前記第1光源と前記第2光源とは電源に電気的に接続され、前記第1光源は前記エアリフト式光合成器の外部に設けられ、前記第2光源は前記エアリフト式光合成器の内部に設けられることを特徴とする、請求項3に記載の工業的に培養可能な藻体の培養システム。 The light source means includes a first light source, a second light source, and a power source, and the first light source and the second light source are electrically connected to a power source, and the first light source is a part of the airlift type photocombiner. The industrially cultivatable algal cell culture system according to claim 3, wherein the system is provided outside, and the second light source is provided inside the airlift photosynthesis device. 更に、前記連結型密閉容器には気体吹込み装置65が接続され、前記気体吹込み装置は、第1配管と、第2配管と、第3配管とを有し、前記第1配管は各小型試験管に連接され、前記第2配管は各中型ビーカーに連接され、前記第3配管は各エアリフト式光合成器に連接されることを特徴とする、請求項3に記載の工業的に培養可能な藻体の培養システム。 Further, a gas blowing device 65 is connected to the connected airtight container, and the gas blowing device has a first pipe, a second pipe, and a third pipe, and the first pipe is small in size. 4. The industrially culturable according to claim 3, wherein the second pipe is connected to each medium-sized beaker, and the third pipe is connected to each airlift type photosynthesis device. Algae culture system. 前記光透過配管の略中間部には、排気するための通気弁部材が設けられ、前記通気弁部材は、前記光透過配管と連通される連通水管と、前記連通水管と連通されるとともに上端の高さを前記光透過配管より高くした排気管と、前記連通水管を開閉するための開閉弁と、排出弁とを有することを特徴とする、請求項1に記載の工業的に培養可能な藻体の培養システム。 A vent valve member for exhausting is provided in a substantially middle portion of the light transmission pipe, and the vent valve member is connected to the light transmission pipe, and is connected to the communication water pipe and at the upper end. The industrially cultivatable algae according to claim 1, comprising an exhaust pipe having a height higher than that of the light transmission pipe, an open / close valve for opening and closing the communication water pipe, and a discharge valve. Body culture system. 更に、連通管手段に、互いに流通させるように配置された複数本の連続式の拡大式連通管が設けられ、各拡大式連通管ごとには気体経路が接続されて、2個で一組とする拡大式連通管の下部には、一組になった二つの拡大式連通管を連結する開閉弁部材が設けられることを特徴とする、請求項1に記載の工業的に培養可能な藻体の培養システム。 Further, the communication pipe means is provided with a plurality of continuous expansion communication pipes arranged so as to circulate with each other, and a gas path is connected to each expansion communication pipe, and two of them are one set. The industrially cultivatable alga body according to claim 1, wherein an opening / closing valve member for connecting two sets of expansion communication pipes is provided at a lower portion of the expansion communication pipe. Culture system. 前記液貯留手段は配管であって、管径は光透過配管の管径より大径で、底部には採集弁が設けられることを特徴とする、請求項1に記載の工業的に培養可能な藻体の培養システム。 2. The industrially cultivatable according to claim 1, wherein the liquid storage means is a pipe, the pipe diameter is larger than that of the light transmission pipe, and a collecting valve is provided at the bottom. Algae culture system. 前記ジェット式酸素排出手段は、互いに取り付けられた酸素排出筒体と集液部とから構成され、前記酸素排出筒体の側壁には、集液部まで延伸された給気管が設けられることを特徴とする、請求項1に記載の工業的に培養可能な藻体の培養システム。 The jet type oxygen discharge means is composed of an oxygen discharge cylinder and a liquid collection part attached to each other, and a side wall of the oxygen discharge cylinder is provided with an air supply pipe extending to the liquid collection part. The culture system for industrially cultivated alga bodies according to claim 1. 更に、前記酸素排出筒体には、排気管が設けられ、前記排気管と中空管の上端には第1通気半密閉素子で通気可能に塞がれることで、酸素排出筒体を外部環境から密閉状態にし、また、前記給気管の開口は前記酸素排出筒体の側に設けられた第2通気半密閉素子で通気可能に塞がれ、酸素排出筒体を外部環境から密閉状態にすることを特徴とする、請求項9に記載の工業的に培養可能な藻体の培養システム。

Further, the oxygen discharge cylinder is provided with an exhaust pipe, and the upper ends of the exhaust pipe and the hollow pipe are closed so as to be ventilated by a first ventilation semi-sealing element, so that the oxygen discharge cylinder is externally surrounded And the opening of the air supply pipe is closed to be ventilated by a second ventilation semi-sealing element provided on the side of the oxygen discharge cylinder, so that the oxygen discharge cylinder is sealed from the external environment. The industrially cultivatable algal cell culture system according to claim 9, wherein:

JP2010230665A 2010-10-13 2010-10-13 Culture system of alga capable of being industrially cultivated Pending JP2012080839A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521042A (en) * 2012-05-17 2015-07-27 エス. レッドフォード,ダニエル Aquatic-based microalgae production equipment
WO2017221591A1 (en) * 2016-06-24 2017-12-28 学校法人 慶應義塾 Cell processing system and cell supply method
KR101831958B1 (en) 2017-01-06 2018-02-23 주식회사 코리아스피루리나 Spirulina culture system
JP2019187318A (en) * 2018-04-25 2019-10-31 三菱重工機械システム株式会社 Algae culture device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015521042A (en) * 2012-05-17 2015-07-27 エス. レッドフォード,ダニエル Aquatic-based microalgae production equipment
WO2017221591A1 (en) * 2016-06-24 2017-12-28 学校法人 慶應義塾 Cell processing system and cell supply method
KR101831958B1 (en) 2017-01-06 2018-02-23 주식회사 코리아스피루리나 Spirulina culture system
JP2019187318A (en) * 2018-04-25 2019-10-31 三菱重工機械システム株式会社 Algae culture device

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