JP2009521228A - 単球の樹状細胞への分化を促進する多孔質膜デバイス - Google Patents
単球の樹状細胞への分化を促進する多孔質膜デバイス Download PDFInfo
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Abstract
【選択図】なし
Description
本出願は、2005年12月21日に出願された米国仮出願第60/752,033号(これは、その全体が参照により本明細書に援用される)の利益を主張する。
多孔質膜の上面上で内皮細胞を培養すること(当該膜は、ウェルの下部チャンバの上方に吊るされ、且つウェルの下部チャンバと分離可能であるウェルの上部チャンバに収容される)、
末梢血単核細胞(PBMC)を、多孔質膜上の内皮細胞へ適用すること、
PBMCの適用の少なくとも約48時間後に、多孔質膜及び内皮細胞を収容しているウェルの上部チャンバを除去すること、並びに
ウェルの下部チャンバから樹状細胞を単離すること、
を含む多数の樹状細胞を生成する方法を提供する。
以下の:
基材としての第1の多孔質膜、
前記第1の多孔質膜の上面上に取り付けられたECM材料、及び
前記ECM材料の上面上に取り付けられた第2の多孔質膜
を含む第1のウェルを作製すること、
前記第1のウェルを、細胞培地を含む第2のウェルへ反転させること、
前記第1の多孔質膜の下面上で内皮細胞を培養すること、
末梢血単核細胞(PBMC)を内皮細胞へ適用すること、
およそ1.5時間後に、前記第1の多孔質膜の下面の前記PBMC及び前記内皮細胞を洗い落とすこと(樹状細胞はこの段階で前記ECM材料中に存在する)、
前記第1のウェルを、細胞培地を含む前記第2のウェルから除去するとともに、前記第2の多孔質膜を伴う前記第1のウェルを、第2のECM材料並びに複数のリンパ球及び白血球を含む三次元人工リンパ組織を基材として含む第3のウェルに上向きに配置すること、
前記第2の多孔質膜の上面へ作用物質を適用すること(前記抗原は、樹状細胞が、前記第1のECM材料から前記三次元人工リンパ組織へと遊走するのを可能にする)、並びに
前記作用物質に対する免疫応答を評価すること、
を含む、作用物質に対する動物の潜在的反応を評価する方法を提供する。
以下の:
基材としての第1の多孔質膜、
前記第1の多孔質膜の下面上で培養された内皮細胞、
前記第1の多孔質膜より上に位置し、且つ前記第1の多孔質膜と分離される第2の多孔質膜、
前記第2の多孔質膜の上面で培養された内皮細胞、及び
前記第1の多孔質膜と前記第2の多孔質膜との間に位置する作用物質を含む細胞培養培地、
を含む第1のウェルを作製すること、
前記第1のウェルを、細胞培地を含む第2のウェルへ反転させること、
末梢血単核細胞(PBMC)を、前記第2の多孔質膜の上面上で培養された内皮細胞へ適用すること、
PBMCの適用の少なくとも約48時間後に、前記第1のウェルを前記第2のウェルから除去すること、及び
樹状細胞を前記第2のウェルから単離すること
を含む、多数の樹状細胞を生成する方法をさらに提供する。
・このアプローチの迅速性(DC分化はほんの約2日で起きる)、
・分化プロセス自体(これは、生理学的条件下でのDCの発達により類似する)、
・系の費用対効果(単球の予めの選択は必要ではなく、DC発達は高価な組換えサイトカインの非存在下で起きるため)
を包含する、in vitroでのサイトカイン駆動型DC発達の現行の方法を上回る幾つかの利点を提供する。
[実施例]
Claims (49)
- 多数の樹状細胞を生成する方法であって、
多孔質膜の上面(top)上で内皮細胞を培養すること(該膜は、ウェルの下部チャンバの上方に吊るされ、且つウェルの下部チャンバと分離可能であるウェルの上部チャンバに収容される)、
末梢血単核細胞(PBMC)を、前記多孔質膜上の前記内皮細胞へ適用すること、
前記PBMCの適用の少なくとも約48時間後に、前記多孔質膜及び前記内皮細胞を収容している前記ウェルの前記上部チャンバを除去すること、並びに
前記ウェルの前記下部チャンバから樹状細胞を単離すること、
を含む多数の樹状細胞を生成する方法。 - 前記多孔質膜はポリカーボネート膜である、請求項1に記載の方法。
- 前記内皮細胞はヒト臍帯静脈内皮細胞(HUVEC)である、請求項1に記載の方法。
- 前記内皮細胞は、形質転換された内皮細胞系である、請求項1に記載の方法。
- 前記樹状細胞は、温培地で前記ウェルを洗浄することにより前記下部チャンバから単離される、請求項1に記載の方法。
- Transwell(登録商標)デバイスは、前記ウェルの前記上部チャンバ、前記ポリカーボネート膜及び前記ウェルの前記下部チャンバを提供するのに使用される、請求項2に記載の方法。
- 前記樹状細胞はCD14陽性である、請求項1に記載の方法。
- 前記多孔質膜は、およそ5μmの孔を有する、請求項1に記載の方法。
- 前記ウェルの前記下部チャンバから前記樹状細胞を単離する前に、作用物質が添加される、請求項1に記載の方法。
- 前記作用物質は、ワクチン、アジュバント、免疫療法候補物、免疫調節物質、化粧品、薬物、生物学的製剤(biologic)、炎症誘発剤及び化合物から成る群から選択される、請求項9に記載の方法。
- 前記内皮細胞は、前記PBMCを添加する前にコンフルエントになるまで培養される、請求項1に記載の方法。
- 前記内皮細胞は、前記PBMCを添加する前に、多層細胞成長が達成されるまで培養される、請求項1に記載の方法。
- 前記ウェルの前記下部チャンバは、細胞外マトリックス(ECM)材料を含む、請求項1に記載の方法。
- 前記ECM材料は、ゼラチン、コラーゲン、合成ECM材料、PLGA、PGA、天然ECM材料、キトサン、プロトサン及びそれらの混合物から成る群から選択される材料を含む、請求項13に記載の方法。
- 前記ウェルの前記下部チャンバは、線維芽細胞をさらに含む、請求項1に記載の方法。
- 前記ウェルの前記下部チャンバは、他の支持細胞をさらに含む、請求項1に記載の方法。
- 前記ウェルの前記下部チャンバは、間質細胞をさらに含む、請求項1に記載の方法。
- 前記内皮細胞は、ECM材料へ付着される、請求項1に記載の方法。
- 前記ECM材料は、ゼラチン、コラーゲン、合成ECM材料、PLGA、PGA、天然ECM材料、キトサン、プロトサン及びそれらの混合物から成る群から選択される材料を含む、請求項18に記載の方法。
- 前記多孔質膜は、多孔性を増大させるようにレーザー微細加工される(laser-micromachined)、請求項1に記載の方法。
- 前記内皮細胞はまた、前記多孔質膜の下面(bottom)上で培養される、請求項1に記載の方法。
- 前記内皮細胞はまた、ECM材料の存在下で前記多孔質膜の下面上で培養される、請求項1に記載の方法。
- 作用物質に対する動物の潜在的反応を評価する方法であって、
以下の:
基材としての第1の多孔質膜、
前記第1の多孔質膜の上面上に取り付けられたECM材料、及び
前記ECM材料の上面上に取り付けられた第2の多孔質膜
を含む第1のウェルを作製すること、
前記第1のウェルを、細胞培地を含む第2のウェルへ反転させること、
前記第1の多孔質膜の下面上で内皮細胞を培養すること、
末梢血単核細胞(PBMC)を前記内皮細胞へ適用すること、
およそ1.5時間後に、前記第1の多孔質膜の前記下面の前記PBMC及び前記内皮細胞を洗い落とすこと(樹状細胞はこの段階で前記ECM材料中に存在する)、
前記第1のウェルを、細胞培地を含む前記第2のウェルから除去し、前記第2の多孔質膜を伴う前記第1のウェルを、第2のECM材料並びに複数のリンパ球及び白血球を含む三次元人工リンパ組織を基材として含む第3のウェルに上向きに配置すること、
前記第2の多孔質膜の前記上面へ作用物質を適用すること(前記抗原は、前記樹状細胞が、前記第1のECM材料から前記三次元人工リンパ組織へと遊走するのを可能にする)、並びに
前記作用物質に対する免疫応答を評価すること、
を含む、作用物質に対する動物の潜在的反応を評価する方法。 - 前記内皮細胞は、ヒト臍帯静脈内皮細胞(HUVEC)である、請求項23に記載の方法。
- 前記内皮細胞は、形質転換された内皮細胞系である、請求項23に記載の方法。
- 前記第1の多孔質膜及び前記第2の多孔質膜は、ポリカーボネート膜である、請求項23に記載の方法。
- 前記第1の多孔質膜及び前記第2の多孔質膜は、およそ5μmの孔を有する、請求項23に記載の方法。
- 前記作用物質は、ワクチン、アジュバント、免疫療法候補物、免疫調節物質、化粧品、薬物、生物学的製剤、炎症誘発剤及び化合物から成る群から選択される、請求項23に記載の方法。
- 前記内皮細胞は、前記PBMCを添加する前にコンフルエントになるまで培養される、請求項23に記載の方法。
- 前記ECM材料は、ゼラチン、コラーゲン、合成ECM材料、PLGA、PGA、天然ECM材料、キトサン、プロトサン及びそれらの混合物から成る群から選択される材料を含む、請求項23に記載の方法。
- 前記第1の多孔質膜及び前記第2の多孔質膜は、多孔性を増大させるようにレーザー微細加工される、請求項23に記載の方法。
- 多数の樹状細胞を生成する方法であって、
以下の:
基材としての第1の多孔質膜、
前記第1の多孔質膜の下面上で培養された内皮細胞、
前記第1の多孔質膜より上に位置し、且つ前記第1の多孔質膜と分離される第2の多孔質膜、
前記第2の多孔質膜の上面で培養された内皮細胞、及び
前記第1の多孔質膜と前記第2の多孔質膜との間に位置する作用物質を含む細胞培養培地
を含む第1のウェルを作製すること、
前記第1のウェルを、細胞培地を含む第2のウェルへ反転させること、
末梢血単核細胞(PBMC)を、前記第2の多孔質膜の前記上面上で培養された前記内皮細胞へ適用すること、
前記PBMCの適用の少なくとも約48時間後に、前記第1のウェルを前記第2のウェルから除去すること、及び
樹状細胞を前記第2のウェルから単離すること、
を含む、多数の樹状細胞を生成する方法。 - 前記内皮細胞はヒト臍帯静脈内皮細胞(HUVEC)である、請求項32に記載の方法。
- 前記内皮細胞は、形質転換された内皮細胞系である、請求項32に記載の方法。
- 前記樹状細胞は、温培地で前記ウェルを洗浄することにより前記第2のウェルから単離される、請求項32に記載の方法。
- Transwell(登録商標)デバイスは、前記第1のウェルを提供するのに使用される、請求項32に記載の方法。
- 前記樹状細胞はCD14陽性である、請求項32に記載の方法。
- 前記多孔質膜は、およそ5μmの孔を有する、請求項32に記載の方法。
- 前記内皮細胞は、前記PBMCを添加する前にコンフルエントになるまで培養される、請求項32に記載の方法。
- 前記内皮細胞は、前記PBMCを添加する前に、多層細胞成長が達成されるまで培養される、請求項32に記載の方法。
- 前記第2のウェルは、該ウェルの前記基材に位置するECM材料を有する、請求項32に記載の方法。
- 前記ECM材料は、ゼラチン、コラーゲン、合成ECM材料、PLGA、PGA、天然ECM材料、キトサン、プロトサン及びそれらの混合物から成る群から選択される材料を含む、請求項41に記載の方法。
- 前記第2のウェルは、該ウェルの前記基材に位置する線維芽細胞を含む、請求項32に記載の方法。
- 前記第2のウェルは、該ウェルの前記基材に位置する支持細胞を含む、請求項32に記載の方法。
- 前記第2のウェルは、該ウェルの前記基材に位置する間質細胞を含む、請求項32に記載の方法。
- 前記内皮細胞は、ECM材料へ結合される、請求項32に記載の方法。
- 前記ECM材料は、ゼラチン、コラーゲン、合成ECM材料、PLGA、PGA、天然ECM材料、キトサン、プロトサン及びそれらの混合物から成る群から選択される材料を含む、請求項46に記載の方法。
- 前記多孔質膜は、多孔性を増大させるようにレーザー微細加工される、請求項32に記載の方法。
- 前記多孔質膜はポリカーボネート膜である、請求項32に記載の方法。
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018537116A (ja) * | 2015-12-17 | 2018-12-20 | ロレアル | いくつかの直径の細孔を含む再構築された組織のための膜 |
JP2019531717A (ja) * | 2016-09-08 | 2019-11-07 | サノフィ パスツール ヴァックスデザイン コーポレーション | インビトロの新生児バイオミメティック(nmimic)モデル及び方法、並びにその使用 |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8298824B2 (en) | 2004-04-28 | 2012-10-30 | Sanofi Pasteur Vaxdesign Corporation | Methods of evaluating a test agent in a diseased cell model |
US8071373B2 (en) | 2004-04-28 | 2011-12-06 | Sanofi Pasteur Vaxdesign Corp. | Co-culture lymphoid tissue equivalent (LTE) for an artificial immune system (AIS) |
US7709256B2 (en) * | 2004-04-28 | 2010-05-04 | Vaxdesign Corp. | Disease model incorporation into an artificial immune system (AIS) |
US7855074B2 (en) * | 2004-04-28 | 2010-12-21 | Vaxdesign Corp. | Artificial immune system: methods for making and use |
US7785883B2 (en) | 2004-04-28 | 2010-08-31 | Vax Design Corp. | Automatable artificial immune system (AIS) |
US7771999B2 (en) | 2004-04-28 | 2010-08-10 | Vaxdesign Corp. | Disease model incorporation into an artificial immune system (AIS) |
US8030070B2 (en) * | 2004-04-28 | 2011-10-04 | Sanofi Pasteur Vaxdesign Corp. | Artificial lymphoid tissue equivalent |
US7785806B2 (en) | 2004-04-28 | 2010-08-31 | Vaxdesign Corporation | Method for determining the immunogenicity of an antigen |
EP1969366B1 (en) | 2005-12-21 | 2015-10-21 | Sanofi Pasteur VaxDesign Corporation | In vitro germinal centers |
ATE495238T1 (de) * | 2005-12-21 | 2011-01-15 | Vaxdesign Corp | Iin vitro methode um eine potentielle reaktion eines tieres an einen agenten zu bestimmen |
EP2409714A1 (en) * | 2006-06-27 | 2012-01-25 | Sanofi Pasteur VaxDesign Corporation | Models for vaccine assessment |
WO2009048661A1 (en) | 2007-07-16 | 2009-04-16 | Vaxdesign Corporation | Artificial tissue constructs comprising alveolar cells and methods for using the same |
WO2010105285A1 (en) * | 2009-03-17 | 2010-09-23 | The Heart Research Institute | Method of cell differentiation and uses therefor in screening, diagnosis and therapy |
US8802747B2 (en) * | 2009-08-26 | 2014-08-12 | Molecular Imprints, Inc. | Nanoimprint lithography processes for forming nanoparticles |
JP2015509382A (ja) | 2012-03-07 | 2015-03-30 | チルドレンズ メディカル センター コーポレーション | 組織構築体およびその使用 |
US9423234B2 (en) | 2012-11-05 | 2016-08-23 | The Regents Of The University Of California | Mechanical phenotyping of single cells: high throughput quantitative detection and sorting |
KR101986092B1 (ko) * | 2016-09-13 | 2019-06-05 | 대한뉴팜(주) | 유두진피 섬유아세포의 고순도 분리 방법 |
CA3068364C (en) * | 2017-06-26 | 2023-09-26 | BIOHOPE Scientific Solutions for Human Health S.L. | Method for predicting and monitoring clinical response to immunomodulatory therapy |
WO2021008472A1 (zh) * | 2019-07-15 | 2021-01-21 | 京东方科技集团股份有限公司 | 细胞片层转移器件、运输装置和转移细胞片层的方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10508522A (ja) * | 1994-11-08 | 1998-08-25 | オーガノジェネシス・インコーポレーテッド | イン・ビトロ組織および器官相当モデル |
WO2005104755A2 (en) * | 2004-04-28 | 2005-11-10 | Vaxdesign Corporation | Artificial immune system: methods for making and use |
Family Cites Families (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5160490A (en) * | 1986-04-18 | 1992-11-03 | Marrow-Tech Incorporated | Three-dimensional cell and tissue culture apparatus |
US5720937A (en) * | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
US5008116A (en) * | 1988-11-14 | 1991-04-16 | Frederick Cahn | Immunostimulatory microsphere |
US5562910A (en) * | 1989-09-25 | 1996-10-08 | University Of Utah Research Foundation | Vaccine compositions and method for enhancing an immune response |
US5188959A (en) * | 1989-09-28 | 1993-02-23 | Trustees Of Tufts College | Extracellular matrix protein adherent t cells |
US5695996A (en) * | 1994-09-23 | 1997-12-09 | The United States Of America As Represented By The Department Of Health And Human Services | Artificial organ culture system |
US6479258B1 (en) * | 1995-12-07 | 2002-11-12 | Diversa Corporation | Non-stochastic generation of genetic vaccines |
US5739001A (en) * | 1996-10-29 | 1998-04-14 | E. I. Du Pont De Nemours And Company | Solid phase cell-based assay |
US6177282B1 (en) * | 1997-08-12 | 2001-01-23 | Mcintyre John A. | Antigens embedded in thermoplastic |
ATE428769T1 (de) * | 1997-10-27 | 2009-05-15 | Univ Rockefeller | Methode und zusammensetzung zur herstellung von reifen dendritischen zellen |
US6835550B1 (en) * | 1998-04-15 | 2004-12-28 | Genencor International, Inc. | Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins |
US20020155108A1 (en) * | 1998-05-04 | 2002-10-24 | Biocrystal, Ltd. | Method for ex vivo loading of antigen presenting cells with antigen, and a vaccine comprising the loaded cells |
MXPA02004666A (es) * | 1999-11-12 | 2003-06-24 | Fibrogen Inc | Gelatinas recombinantes. |
CN1423523A (zh) * | 1999-11-17 | 2003-06-11 | 罗切斯特大学 | 来自人体内的免疫系统 |
US6479064B1 (en) * | 1999-12-29 | 2002-11-12 | Children's Medical Center Corporation | Culturing different cell populations on a decellularized natural biostructure for organ reconstruction |
US6541225B1 (en) * | 2000-01-26 | 2003-04-01 | Raven Biotechnologies, Inc. | Methods and compositions for generating human monoclonal antibodies |
US20050164388A1 (en) * | 2001-02-07 | 2005-07-28 | Young-Sook Son | Method of isolating epithelial cells, method of preconditioning cells, and methods of preparing bioartificial skin and dermis with the epithelial cells and preconditioned cells |
EP1437147B1 (en) * | 2001-09-25 | 2012-06-27 | National Institute for Environmental Studies | Method of preparing basement membrane |
FR2833271B1 (fr) * | 2001-12-10 | 2004-09-17 | Coletica | Production de cellules dendritiques in vitro a partir de monocytes cd14+, pour notamment la realisation de modeles cellulaires et/ou tissulaires en suspension, en monocouches et tridimentionnels; utilisation de ces modeles |
GB0130789D0 (en) * | 2001-12-21 | 2002-02-06 | King S College London | Application of spores |
AU2003265229A1 (en) * | 2002-03-18 | 2003-12-12 | Sciperio, Inc. | Dentritic cell nodes |
US7476514B2 (en) * | 2002-04-11 | 2009-01-13 | Cylex, Inc. | Method for monitoring the immune response and predicting clinical outcomes in transplant recipients |
US20040009943A1 (en) * | 2002-05-10 | 2004-01-15 | Inex Pharmaceuticals Corporation | Pathogen vaccines and methods for using the same |
US20050191743A1 (en) * | 2002-10-03 | 2005-09-01 | Wu J.H. D. | Three-dimensional peripheral lymphoid organ cell cultures |
US20040109876A1 (en) * | 2002-11-25 | 2004-06-10 | Kureha Chemical Industry Co., Ltd. | Vaccine composition, HIV-infection suppression factor and method for the vaccination against HIV |
US20070141552A1 (en) * | 2004-04-28 | 2007-06-21 | Warren William L | Automatable artificial immune system (AIS) |
US20060275270A1 (en) * | 2004-04-28 | 2006-12-07 | Warren William L | In vitro mucosal tissue equivalent |
US7785806B2 (en) * | 2004-04-28 | 2010-08-31 | Vaxdesign Corporation | Method for determining the immunogenicity of an antigen |
US8030070B2 (en) * | 2004-04-28 | 2011-10-04 | Sanofi Pasteur Vaxdesign Corp. | Artificial lymphoid tissue equivalent |
US7785883B2 (en) * | 2004-04-28 | 2010-08-31 | Vax Design Corp. | Automatable artificial immune system (AIS) |
US8071373B2 (en) * | 2004-04-28 | 2011-12-06 | Sanofi Pasteur Vaxdesign Corp. | Co-culture lymphoid tissue equivalent (LTE) for an artificial immune system (AIS) |
US7771999B2 (en) * | 2004-04-28 | 2010-08-10 | Vaxdesign Corp. | Disease model incorporation into an artificial immune system (AIS) |
US7855074B2 (en) * | 2004-04-28 | 2010-12-21 | Vaxdesign Corp. | Artificial immune system: methods for making and use |
ATE495238T1 (de) * | 2005-12-21 | 2011-01-15 | Vaxdesign Corp | Iin vitro methode um eine potentielle reaktion eines tieres an einen agenten zu bestimmen |
EP1969366B1 (en) * | 2005-12-21 | 2015-10-21 | Sanofi Pasteur VaxDesign Corporation | In vitro germinal centers |
EP2409714A1 (en) * | 2006-06-27 | 2012-01-25 | Sanofi Pasteur VaxDesign Corporation | Models for vaccine assessment |
EP2420516A1 (en) * | 2007-07-06 | 2012-02-22 | Sanofi Pasteur VaxDesign Corporation | Rapid generation of T cell-independent antibody responses to T cell-dependent antigens |
-
2006
- 2006-12-21 AT AT06848082T patent/ATE495238T1/de not_active IP Right Cessation
- 2006-12-21 AU AU2006330951A patent/AU2006330951B2/en active Active
- 2006-12-21 AT AT10151003T patent/ATE531793T1/de active
- 2006-12-21 DE DE602006019638T patent/DE602006019638D1/de active Active
- 2006-12-21 US US11/642,926 patent/US20070178076A1/en not_active Abandoned
- 2006-12-21 CA CA2634119A patent/CA2634119C/en active Active
- 2006-12-21 EP EP10151003A patent/EP2199384B1/en active Active
- 2006-12-21 EP EP06848082A patent/EP1963491B1/en active Active
- 2006-12-21 WO PCT/US2006/049128 patent/WO2007076061A1/en active Application Filing
- 2006-12-21 JP JP2008547637A patent/JP2009521228A/ja active Pending
- 2006-12-21 CA CA2847310A patent/CA2847310A1/en not_active Abandoned
-
2008
- 2008-06-12 IL IL192090A patent/IL192090A/en active IP Right Grant
-
2009
- 2009-10-30 US US12/609,060 patent/US20100105135A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10508522A (ja) * | 1994-11-08 | 1998-08-25 | オーガノジェネシス・インコーポレーテッド | イン・ビトロ組織および器官相当モデル |
WO2005104755A2 (en) * | 2004-04-28 | 2005-11-10 | Vaxdesign Corporation | Artificial immune system: methods for making and use |
Non-Patent Citations (7)
Title |
---|
JPN6012017130; 血液フロンティア Vol.11, No.8, 2001, p.47-52 * |
JPN6012017132; Science Vol.282, 1998, p.480-483 * |
JPN6012017134; Blood Vol.106, 20050719, p.2818-2826 * |
JPN6012017136; Arthritis Rheumatism Vol.52, No.11, 200511, p.3460-3469 * |
JPN6013000799; J.Immunol. Vol.160, 1998, p.2675-2683 * |
JPN6013000802; Immunity Vol.13, 2000, p.323-332 * |
JPN6013000805; Eur.J.Immunol. Vol.26, 1996, p.759-767 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2018537116A (ja) * | 2015-12-17 | 2018-12-20 | ロレアル | いくつかの直径の細孔を含む再構築された組織のための膜 |
JP2019531717A (ja) * | 2016-09-08 | 2019-11-07 | サノフィ パスツール ヴァックスデザイン コーポレーション | インビトロの新生児バイオミメティック(nmimic)モデル及び方法、並びにその使用 |
JP2021180673A (ja) * | 2016-09-08 | 2021-11-25 | サノフィ パスツール ヴァックスデザイン コーポレーション | インビトロの新生児バイオミメティック(nmimic)モデル及び方法、並びにその使用 |
JP7274540B2 (ja) | 2016-09-08 | 2023-05-16 | サノフィ パスツール ヴァックスデザイン コーポレーション | インビトロの新生児バイオミメティック(nmimic)モデル及び方法、並びにその使用 |
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CA2634119A1 (en) | 2007-07-05 |
AU2006330951B2 (en) | 2012-04-05 |
EP1963491B1 (en) | 2011-01-12 |
CA2634119C (en) | 2015-02-24 |
ATE495238T1 (de) | 2011-01-15 |
CA2847310A1 (en) | 2007-07-05 |
DE602006019638D1 (de) | 2011-02-24 |
US20070178076A1 (en) | 2007-08-02 |
AU2006330951A1 (en) | 2007-07-05 |
EP1963491A1 (en) | 2008-09-03 |
US20100105135A1 (en) | 2010-04-29 |
EP2199384B1 (en) | 2011-11-02 |
WO2007076061A1 (en) | 2007-07-05 |
ATE531793T1 (de) | 2011-11-15 |
EP2199384A1 (en) | 2010-06-23 |
IL192090A0 (en) | 2008-12-29 |
IL192090A (en) | 2012-01-31 |
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