JP2009216519A - Micro-reaction vessel for blood examination - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a micro-reaction vessel for blood examination can more accurate examination by removing a non-specific reaction in the blood examination. <P>SOLUTION: This micro-reaction vessel for the blood examination includes a lower storage part having inert particles and an antibody, and an upper storage part into which a specimen is injected. The upper storage part includes an opening part, and a leukocyte removing filter between the opening part and the lower storage part. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a micro reaction container for blood test.

  In recent years, centrifugal agglutination methods using columns and microtubes have been used as means for performing biological tests such as blood tests. For example, in Patent Document 1, an inactive particle typified by 10 to 200 μm polymer particles or glass particles is filled in an elongated micro reaction vessel, and a reaction vessel capable of efficiently distinguishing erythrocyte aggregates and non-aggregates by centrifugation is provided. Disclosure. Patent Document 2 discloses a similar reaction vessel using glass beads. Each method has a shape in which a plurality of elongated microreaction containers filled with inert particles are embedded in a plate-like plastic plate, and is used in a state where the microreaction containers are set up in a vertical direction.

  In the analysis, a liquid sample is injected from an upper opening of a vertically arranged micro reaction vessel, and the opening has a funnel shape and is used as a reaction vessel. The reaction antibody is contained in the upper part of the inert particle layer, and the sample is reacted with the antibody, and then centrifuged. When an aggregate is generated by the reaction, the aggregate is captured by the inert particle layer to form a positive image. If no aggregate is formed, the injected sample passes through the inert particle layer and moves to the bottom of the column to form a negative image. By capturing the aggregates with the inert particles in this way, it is possible to easily test without washing the red blood cells (B / F separation).

However, in a conventional blood test using a microreaction container, non-specific reactions such as impurities remaining in the upper layer of inert particles or partial agglomeration may occur, and accurate testing may not be possible.
Japanese Patent Publication No.8-7215 European Patent No. 725276

  In view of the above problems, an object of the present invention is to provide a micro reaction container for blood test that eliminates non-specific reaction in blood test and enables more accurate test.

  According to the present invention, it is a micro reaction container for blood test, comprising a lower housing part provided with inert particles and an antibody, and an upper housing part into which a specimen is injected, the upper housing part having an opening, A micro reaction container for blood test is provided, comprising a leukocyte removal filter between the opening and the lower housing. In one embodiment, the leukocyte removal filter is detachably attached to the reaction container. In one embodiment, the leukocyte removal filter is carried on a filter carrier and attached to the reaction vessel.

  In one embodiment, the opening is sealed with a hermetic seal, and the filter carrier is detachably mounted simultaneously with the hermetic seal.

  In one embodiment, the inert particles are selected from the group consisting of polymers, gels, glass beads, silica, dextran, and gelatin particles.

  In a further aspect, there is provided the above micro reaction container for blood test, which is fixed to a support that can be subjected to centrifugation. In one embodiment, the micro reaction container for blood test is provided, which is formed integrally with the support. In one embodiment, the support has a flat card shape.

  From another aspect of the present invention, there is provided a blood test card comprising a plurality of the above micro test containers for blood test.

  According to still another aspect of the present invention, there is provided a filter carrier that carries a leukocyte removal filter that is attached to a blood reaction microreaction container.

  According to the micro reaction container for blood test of the present invention, non-specific reaction in the blood test is eliminated, and a more accurate test can be easily performed.

  In a blood test using a micro reaction container using the centrifugal agglutination method, a reaction image becomes unclear and it may be difficult to determine a positive image or a negative image. The present inventors examined the occurrence of a non-specific reaction as one of the causes that make it difficult to discriminate the reaction image, and as a result of intensive studies, this non-specific reaction is caused by leukocytes mixed in the specimen. I found out. Conventionally, specimens for blood tests are serum, plasma, and red blood cells, and washing or the like is performed when these are isolated from whole blood. At this time, white blood cells should have been removed from the specimen. Thus, there has never been a problem with leukocyte contamination in a test using a column.

  However, if the sample added to the micro reaction vessel contains a leukocyte component, leukocytes may remain in the upper layer of the inactive particles even after centrifugation. There is also the possibility of staying in the department. This is thought to make the reaction image unclear.

  Therefore, the present invention provides a micro reaction container for blood test that can perform a more accurate test by providing a micro reaction container using a centrifugal agglutination method with a function for removing leukocyte components in a specimen. . According to this, it is possible to easily remove leukocytes in a specimen without separately performing an operation for removing leukocytes.

  FIG. 1 is a cross-sectional view showing an embodiment of a micro reaction container for blood test according to the present invention. The microreaction container 1 of the present invention comprises a lower housing part 3 comprising inert particles 2 and antibodies, and an upper housing part 5 into which a specimen and optionally a reagent are injected, the upper housing part comprising an opening, A leukocyte removal filter 7 is provided between the opening and the lower housing. The opening is preferably sealed until it is in use, for example sealed by a peelable seal 9. At the time of use, the sealing seal 9 is peeled off and the specimen is added. An aluminum seal is preferably used as the hermetic seal, but is not limited thereto.

  The microreaction container 1 of the present invention may be any shape as long as it is elongated in the vertical direction and can be subjected to centrifugation and the agglutination reaction image can be observed, and the size can also be selected depending on the specimen. Is possible.

  The lower container 3 is filled with inert particles 2 for forming an agglutination reaction image. The inert particles 2 can be selected from suitable particles such as polymers, gels such as Sephadex gel and polyacrylamide gel, glass beads, silica, dextran, and gelatin particles.

  The upper layer portion of the inert particles 2 contains an antibody for reaction. When the specimen is erythrocytes, this antibody reacts with erythrocytes, and when positive, aggregates are formed. For example, in the case of a blood type test, anti-A, anti-B and anti-D antibodies are added in advance to separate reaction containers. When the sample is an antibody, a reagent (red blood cells) is further added together with the sample. When the sample is positive, the sample, reagent, and antibody form an aggregate. The sample and optionally the reagent are added, and then subjected to centrifugation, whereby the aggregate or non-aggregate after the reaction settles through the filled inert particles. If the reaction is positive, the aggregate remains in the upper layer of the inert particles and forms a positive image. On the other hand, if the reaction is negative, no agglomerates are formed and the specimen settles at the bottom of the reaction vessel, forming a negative image.

The reaction result can be discriminated visually, but can also be discriminated using an automatic reading device.

  The upper accommodating portion 5 accommodates a sample or reagent administered from the opening and provides a space for reacting. In the present invention, the upper housing part 5 is provided with a leukocyte removal filter. The leukocyte removal filter is a filter for removing leukocytes from the leukocyte-containing preparation, and conventionally known ones can be used.

  As shown in FIG. 1, the leukocyte removal filter 7 can be provided at any position between the hermetic seal 9 and the inert particles 2. However, it is necessary to secure a space in the upper part of the filter that can store the specimen and the reagent. It is also possible to arrange the leukocyte removal filter 7 in contact with the inert particles 2.

  The administered specimen remains on top of the leukocyte removal filter 7, passes through the leukocyte removal filter 7 by centrifugation, and moves onto the inactive particles 2. At this time, white blood cells are removed from the specimen. The specimen that has passed through the filter 7 reacts with the antibody on the inactive particles 2 as it is and settles to form a reaction image.

  FIG. 2 is a cross-sectional view showing another embodiment of the present invention. In this aspect, the leukocyte removal filter 7 is detachably mounted. For example, when it is not necessary to use the leukocyte removal filter 7 such as when the amount of the sample is very small or when it is confirmed that the leukocyte is not completely contained in the sample, the leukocyte removal filter 7 is removed and the test is performed. Is possible.

  As shown in FIG. 2, the leukocyte removal filter 7 is carried on a filter carrier 11 and attached to a reaction vessel. In one embodiment, as shown in FIG. 3, the filter carrier 11 includes a cylindrical body 11a that fits in the reaction vessel, and a fixing portion 11b that supports the filter carrier 11 at the reaction vessel opening. The filter 7 is provided at the bottom.

  When the leukocyte removal filter 7 is not used, the leukocyte removal filter can be easily removed by removing the filter carrier 11. The micro reaction vessel 1 is sealed by a sealing seal 9 in a state where the filter carrier 11 is mounted. When the sealing seal 9 is peeled off, the micro reaction container 1 can be removed by peeling the filter carrier 11 simultaneously.

  In yet another embodiment of the present invention, the microreaction vessel is fixed to a support that can be subjected to centrifugation. In one embodiment, a plurality of micro reaction vessels are immobilized on the support. For example, the cartridge may be used with a micro reaction vessel. In the case of mounting on a cartridge, for the convenience of reading the reaction image, it is preferable that the micro reaction containers are arranged in a row, and a cartridge arranged in a linear or cylindrical shape is preferably used.

  In one embodiment, the support and the micro reaction vessel are integrally formed. In a preferred embodiment, the support is formed in a flat card shape and can be provided as a blood test card. When the support has a flat card shape, the card body can be copied by a copying machine after the test, and the determination result can be stored for a long time.

  FIG. 4 is a diagram showing an embodiment of the blood test card 20. A filter carrier 15 is mounted on the card 20 and is sealed with a hermetic seal 13 from above. In use, the sealing seal 13 is peeled off and the specimen is added to each reaction vessel 1. FIG. 5 shows a schematic diagram of the filter carrier 15. 5a is a cross-sectional view and FIG. 5b is a plan view. The filter carrier 15 can be formed integrally with filters for all the micro reaction vessels provided on the card. When the leukocyte removal filter is not used, the filter carrier 15 can be peeled off together with the hermetic seal 13, and the filter can be easily removed.

  As described above, according to the micro reaction container for blood test of the present invention, it is possible to remove white blood cells in a specimen without separately performing an operation for removing white blood cells, thereby easily reducing non-specific reactions. It is possible to conduct an accurate inspection. The leukocyte removal filter and the filter carrier are not limited to the above-described embodiment, and can have an appropriate shape depending on the reaction container.

  Furthermore, from another aspect of the present invention, there is provided a filter carrier carrying a leukocyte removal filter attached to a micro reaction container for blood test. Thereby, it is possible to give a leukocyte removal function to a commonly used micro reaction container for blood test.

The schematic diagram which shows the one aspect | mode of a micro reaction container. The schematic diagram which shows the other aspect of a micro reaction container. The schematic diagram which shows one Embodiment of a leukocyte removal filter carrier. The schematic diagram which shows one Embodiment of the card | curd for blood tests. The schematic diagram which shows the filter carrier for the card | curd for blood tests.

Explanation of symbols

  DESCRIPTION OF SYMBOLS 1 ... Micro reaction container, 2 ... Inactive particle, 3 ... Lower accommodating part, 5 ... Upper accommodating part, 7 ... Leukocyte removal filter, 9, 13 ... Sealing seal, 11 ... Filter carrier, 20 ... Card for blood test.

Claims (10)

A micro reaction vessel for blood testing,
A lower container containing inert particles and an antibody, and an upper container into which the specimen is injected, the upper container having an opening, and a leukocyte removal filter between the opening and the lower container. A microreaction container for blood testing, comprising:   The micro reaction container for blood test according to claim 1, wherein the leukocyte removal filter is detachably mounted.   The blood reaction microreaction container according to claim 1 or 2, wherein the leukocyte removal filter is carried on a filter carrier and attached to the reaction container.   The micro reaction container for blood test according to claim 3, wherein the opening is sealed with a hermetic seal, and the filter carrier can be peeled off simultaneously with the hermetic seal.   The micro reaction vessel for blood test according to any one of claims 1 to 4, wherein the inert particles are selected from the group consisting of polymers, gels, glass beads, silica, dextran, and gelatin particles.   The micro reaction container for blood test according to any one of claims 1 to 5, wherein the micro reaction container is fixed to a support that can be subjected to centrifugation.   The micro reaction container for blood test according to claim 6, wherein the micro reaction container is formed integrally with the support.   The micro reaction container for blood test according to claim 6 or 7, wherein the support has a flat card shape.   A card for blood test comprising a plurality of micro reaction containers for blood test according to any one of claims 1 to 5.   A filter carrier carrying a leukocyte removal filter, which is attached to a micro reaction container for blood test.

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