JP2008501795A5 - - Google Patents
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- JP2008501795A5 JP2008501795A5 JP2007527196A JP2007527196A JP2008501795A5 JP 2008501795 A5 JP2008501795 A5 JP 2008501795A5 JP 2007527196 A JP2007527196 A JP 2007527196A JP 2007527196 A JP2007527196 A JP 2007527196A JP 2008501795 A5 JP2008501795 A5 JP 2008501795A5
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- JP
- Japan
- Prior art keywords
- cells
- stem cell
- isolated
- extract
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cell Anatomy 0.000 claims 13
- 238000000034 method Methods 0.000 claims 8
- 238000004113 cell culture Methods 0.000 claims 6
- 102000006495 integrins Human genes 0.000 claims 6
- 108010044426 integrins Proteins 0.000 claims 6
- 210000000130 stem cell Anatomy 0.000 claims 6
- 102000016971 Proto-Oncogene Proteins c-kit Human genes 0.000 claims 4
- 108010014608 Proto-Oncogene Proteins c-kit Proteins 0.000 claims 4
- 239000002771 cell marker Substances 0.000 claims 3
- 210000004602 germ cell Anatomy 0.000 claims 3
- 210000001368 germline stem cell Anatomy 0.000 claims 3
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims 2
- 102100027754 Mast/stem cell growth factor receptor Kit Human genes 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- 230000004936 stimulating effect Effects 0.000 claims 2
- XAUDJQYHKZQPEU-KVQBGUIXSA-N 5-aza-2'-deoxycytidine Chemical compound O=C1N=C(N)N=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 XAUDJQYHKZQPEU-KVQBGUIXSA-N 0.000 claims 1
- 101000976622 Homo sapiens Zinc finger protein 42 homolog Proteins 0.000 claims 1
- RTKIYFITIVXBLE-UHFFFAOYSA-N Trichostatin A Natural products ONC(=O)C=CC(C)=CC(C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-UHFFFAOYSA-N 0.000 claims 1
- 102100023550 Zinc finger protein 42 homolog Human genes 0.000 claims 1
- 230000004069 differentiation Effects 0.000 claims 1
- 210000001671 embryonic stem cell Anatomy 0.000 claims 1
- 210000000604 fetal stem cell Anatomy 0.000 claims 1
- 229940121372 histone deacetylase inhibitor Drugs 0.000 claims 1
- 239000003276 histone deacetylase inhibitor Substances 0.000 claims 1
- 230000002611 ovarian Effects 0.000 claims 1
- 230000035755 proliferation Effects 0.000 claims 1
- 230000002381 testicular Effects 0.000 claims 1
- RTKIYFITIVXBLE-QEQCGCAPSA-N trichostatin A Chemical compound ONC(=O)/C=C/C(/C)=C/[C@@H](C)C(=O)C1=CC=C(N(C)C)C=C1 RTKIYFITIVXBLE-QEQCGCAPSA-N 0.000 claims 1
Claims (7)
出生後被験者から生殖細胞サンプルを採取する工程、Collecting a germ cell sample from a postnatal subject,
前記生殖細胞サンプルからｃ−ｋｉｔネガティブ且つαC-kit negative and α from the germ cell sample ６6 インテグリンポジティブである細胞を単離する工程、Isolating cells that are integrin positive,
単離されたｃ−ｋｉｔネガティブ且つαIsolated c-kit negative and α ６6 インテグリンポジティブの細胞を、５−アザ−２’−デオキシシチジン、ヒストンデアセチラーゼインヒビター、ｎ−酪酸、トリコスタチンＡ、細胞全体抽出物、細胞質体抽出物および核質抽出物からなる群から選択される刺激因子を含有する培地で、生育および増殖に有効な条件下で培養することにより、生殖細胞系幹細胞培養物を製造する工程Integrin positive cells are selected from the group consisting of 5-aza-2′-deoxycytidine, histone deacetylase inhibitor, n-butyric acid, trichostatin A, whole cell extract, cytoplast extract and nucleoplasm extract. Producing a germline stem cell culture by culturing in a medium containing a stimulating factor under conditions effective for growth and proliferation
を包含し、Including
該生殖細胞系幹細胞培養物は自己再生およびさらに分化決定された細胞系列へと分化する能力を有する、前記方法。Said method wherein said germline stem cell culture is capable of self-renewal and further differentiation into a determined cell lineage.
少なくとも一つの多能性幹細胞マーカーを発現するｃ−ｋｉｔネガティブ且つαC-kit negative and α expressing at least one pluripotent stem cell marker ６6 インテグリンポジティブの細胞を含み、該幹細胞培養物は自己再生およびさらに分化決定された細胞系列へ分化する能力を有する、前記培養物。Said culture comprising integrin positive cells, said stem cell culture having the ability to self-renew and further differentiate into a determined cell lineage.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/864,788 US20050090004A1 (en) | 2003-01-16 | 2004-06-08 | Stem cell maturation for all tissue lines |
| US58814604P | 2004-07-15 | 2004-07-15 | |
| PCT/US2005/005052 WO2005123123A2 (en) | 2004-06-08 | 2005-02-16 | Therapeutic reprogramming, hybrid stem cells and maturation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008501795A JP2008501795A (en) | 2008-01-24 |
| JP2008501795A5 true JP2008501795A5 (en) | 2008-04-03 |
Family
ID=35355246
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007527196A Withdrawn JP2008501795A (en) | 2004-06-08 | 2005-02-16 | Therapeutic reprogramming and maturation of hybrid stem cells |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1758989A2 (en) |
| JP (1) | JP2008501795A (en) |
| AU (1) | AU2005253923A1 (en) |
| BR (1) | BRPI0511889A (en) |
| CA (1) | CA2567692A1 (en) |
| RU (1) | RU2006147263A (en) |
| WO (1) | WO2005123123A2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5491871B2 (en) | 2007-02-28 | 2014-05-14 | アドビナス セラピュティックス プライベート リミテッド | 2,2,2-Trisubstituted acetamide derivatives as glucokinase activators, methods and pharmaceutical applications thereof |
| EP2155720B1 (en) | 2007-06-08 | 2013-07-17 | Advinus Therapeutics Private Limited | Pyrrole-2-carboxamide derivatives as glucokinase activators, their process and pharmaceutical application |
| EP2090649A1 (en) | 2008-02-13 | 2009-08-19 | Fondazione Telethon | Method for reprogramming differentiated cells |
| EP2402327B1 (en) | 2010-06-29 | 2018-03-07 | Impetis Biosciences Ltd. | Acetamide compounds as glucokinase activators, their process and medicinal applications |
| EP2796873A1 (en) * | 2013-04-25 | 2014-10-29 | QGel SA | Method for a cell-based drug screening assay and the use thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1100870B1 (en) * | 1998-07-31 | 2008-01-02 | Genzyme Corporation | Improvement of cardiac function by mesenchymal stem cell transplantation |
| US20020136709A1 (en) * | 2000-12-12 | 2002-09-26 | Nucleus Remodeling, Inc. | In vitro-derived adult pluripotent stem cells and uses therefor |
| JP2004248505A (en) * | 2001-09-21 | 2004-09-09 | Norio Nakatsuji | Undifferentiated fusion cell of somatic cell derived from es cell deficient in part or all of transplantation antigen and method for producing the same |
| CA2474766A1 (en) * | 2002-01-16 | 2003-07-31 | Chauncey Sayre | Stem cell maturation for all tissue types |
| WO2003102126A1 (en) * | 2002-05-31 | 2003-12-11 | Apollo Life Sciences Pty Ltd | A method of cell therapy using fused cell hybrids |
| WO2004046312A2 (en) * | 2002-11-15 | 2004-06-03 | The Board Of Trustees Of The University Of Illinois | Methods for in vitro expansion of hematopoietic stem cells |
-
2005
- 2005-02-16 CA CA002567692A patent/CA2567692A1/en not_active Abandoned
- 2005-02-16 JP JP2007527196A patent/JP2008501795A/en not_active Withdrawn
- 2005-02-16 AU AU2005253923A patent/AU2005253923A1/en not_active Abandoned
- 2005-02-16 EP EP05723204A patent/EP1758989A2/en not_active Withdrawn
- 2005-02-16 WO PCT/US2005/005052 patent/WO2005123123A2/en active Application Filing
- 2005-02-16 RU RU2006147263/14A patent/RU2006147263A/en unknown
- 2005-02-16 BR BRPI0511889-1A patent/BRPI0511889A/en not_active IP Right Cessation
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