JP2008203216A - Biosensor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biosensor having inexpensive and simple construction for detecting the reaction of a measured specimen or a change in the concentration thereof while utilizing a surface plasmon resonance phenomenon. <P>SOLUTION: The biosensor is provided for detecting interaction between materials while applying the surface plasmon resonance phenomenon. It includes glass rod 2, a metal layer 3 formed on the outer periphery of the glass rod 2 and a first single end face 2a thereof, a sensor part 1 formed on the metal layer 3 and having a reaction layer 4 containing a reactive material for interacting with a specified material, a light source 5 for introducing a light into a second single end face 2b of the glass rod 2, a beam splitter 7 for splitting the introduced light introduced into the glass rod 2 and a plasmon resonance light reflected by the reaction layer 4 formed on the metal layer 3 and the interaction between the materials due to the surface plasmon resonance phenomenon, and a light detector 8 for detecting the light split by the beam splitter 7. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a biosensor that detects a reaction or a change in concentration of a sample to be measured by utilizing a surface plasmon resonance phenomenon.

  As a sensor that can monitor an antigen-antibody reaction in a non-labeled manner in real time, a sensor has been developed that electrically analyzes various intermolecular interactions that occur in a living body using surface plasmon resonance (SPR).

  The surface plasmon resonance phenomenon is that an evanescent wave generated by light of a specific wavelength resonates with electronic vibrations on the surface of a metal thin film when white light is incident on a glass having a metal thin film with a thickness of about 50 nm formed on the surface. It is a phenomenon.

  Since the sensor using this SPR is sensitive to the surface state (refractive index) of the sensor surface, the reaction occurring on the sensor surface can be detected in real time, and the types of components such as the medium (gas, liquid) in contact with the sensor surface Since minute changes due to differences in concentration can be detected, application as a sensor for substance identification and substance concentration measurement is also expected.

  Sensors that measure the interaction between substances by applying this SPR phenomenon have been developed, and one of them is an optical fiber (see, for example, Patent Document 1). This optical fiber sensor removes the grad at the tip of the optical fiber, forms a metal thin film on the surface of the exposed optical fiber core, and further interacts with a specific substance on the metal thin film, such as an antibody. The concentration of the antigen in the sample can be measured by detecting the change in the attenuation wavelength associated with the binding between the antibody and the foreign antigen, or the amount of light attenuation.

Also, a curved portion formed by bending an intermediate portion of the optical fiber composed of the core and the clad into a U-shape, and a metal thin film layer made of a conductive metal so as to cover the exposed core surface by removing the clad of the curved portion. By forming the surface plasmon resonance sensor probe provided, the refractive index of light propagating in the core of the optical fiber changes due to the action of the evanescent wave and the surface plasmon, and is output as a change in emitted light intensity from the other end of the optical fiber. In addition, a surface plasmon resonance sensor probe that can specify the sample to be measured by obtaining the maximum resonance wavelength at which the intensity of the emitted light is attenuated most is disclosed (for example, see Patent Document 2). This surface plasmon resonance sensor probe can detect a minute change in refractive index by completely bending the optical fiber or turning it 180 degrees.
Japanese Patent No. 3592065 JP 2006-105670 A

  However, as in Patent Document 1 and Patent Document 2, conventional optical fiber sensors are difficult to handle and process, such as being easily scratched when removing the cladding, and are expensive because they use expensive special optical fibers. It is difficult to manufacture in large quantities.

  In recent years, there has been an increasing demand for downsizing and cost reduction of optical fiber sensors in order to measure many kinds of substances contained in minute amounts in a minute amount of sample. However, special optical fibers are not popular because they are expensive.

  The present invention has been made in view of such problems, and can detect a reaction or a change in concentration of a sample to be measured using a surface plasmon resonance phenomenon. An object is to provide a sensor.

  In order to achieve the above object, a biosensor according to claim 1 of the present invention is a biosensor that detects an interaction between substances by applying a surface plasmon resonance phenomenon, and includes a glass rod and an outer periphery of the glass rod. And a metal layer formed on the first end surface and a reaction layer formed on the metal layer and containing a reactive substance that interacts with a specific substance.

  The biosensor according to claim 2 of the present invention is the biosensor according to claim 1, wherein a light source that introduces light into the second end face of the glass rod, introduced light introduced into the glass rod, and A beam splitter that branches the plasmon resonance light reflected by the interaction between the reaction layer formed on the metal layer and a substance due to a surface plasmon resonance phenomenon; and a photodetector that detects the light branched by the beam splitter. It is further provided with the feature.

  The biosensor according to claim 3 of the present invention is the biosensor according to claim 1 or 2, wherein the metal layer formed on the outer peripheral portion and one end surface of the glass rod is Au (gold). To do.

  The biosensor according to claim 4 of the present invention is the biosensor according to claim 2, wherein the sensor unit, the light source, the beam splitter, and the photodetector are optically connected in a spatial system. It is characterized by.

  The biosensor according to claim 5 of the present invention is a biosensor for detecting an interaction between substances by applying a surface plasmon resonance phenomenon, and has a first end surface and a second end surface, and has a curved shape. A sensor unit having a glass rod, a metal layer formed only on an outer periphery of the glass rod, and a reaction layer formed on the metal layer and containing a reactive substance that interacts with a specific substance. Features.

  The biosensor according to claim 6 of the present invention is the biosensor according to claim 5, wherein a light source for introducing light into the first one end face of the glass rod, introduced light introduced into the glass rod, and And a photodetector for detecting the reaction layer formed on the metal layer and the plasmon resonance light reflected by the interaction between substances due to a surface plasmon resonance phenomenon from the second end surface of the glass rod. Features.

  The biosensor according to claim 7 of the present invention is the biosensor according to claim 5 or 6, characterized in that the metal layer formed on the outer periphery of the glass rod is Au (gold).

  The biosensor according to claim 8 of the present invention is the biosensor according to claim 6, wherein the sensor unit, the light source, and the photodetector are optically connected in a spatial system. .

  A biosensor according to a ninth aspect of the present invention is the biosensor according to any one of the fifth to eighth aspects, wherein the vicinity of the first one end surface and the vicinity of the second one end surface of the glass rod having a curved shape. The substrate member further includes a substrate member having a curved groove equal to the radius of curvature of the curved shape of the glass rod, and the reaction layer formed on the metal layer is exposed to the outside of the substrate member. To do.

  According to the biosensor of the present invention, it is possible to provide a biosensor that can detect a reaction of a sample to be measured, a change in concentration, and the like using a surface plasmon resonance phenomenon with an inexpensive and simple configuration.

  Next, embodiments of the present invention will be described with reference to the drawings. In the following description of the drawings, the same or similar parts are denoted by the same or similar reference numerals. However, it should be noted that the drawings are schematic and different from the actual ones. Moreover, it is a matter of course that portions having different dimensional relationships and ratios are included between the drawings.

  Further, the embodiment described below exemplifies an apparatus for embodying the technical idea of the present invention, and the technical idea of the present invention is to arrange the components and the like as follows. Not specific. The technical idea of the present invention can be variously modified within the scope of the claims.

[First embodiment]
As shown in FIG. 1, the biosensor according to the first embodiment of the present invention is a biosensor that detects an interaction between substances by applying a surface plasmon resonance phenomenon, and includes a glass rod 2 and a glass rod. 2 includes a sensor layer 1 having a metal layer 3 formed on the outer peripheral portion 2 and the first one end face 2a, and a reaction layer 4 formed on the metal layer 3 and containing a reactive substance that interacts with a specific substance.

  Furthermore, the biosensor according to the first embodiment of the present invention includes a light source 5 for introducing light into the second end face 2b of the glass rod 2 and an introduction introduced into the glass rod 2, as shown in FIG. Light and a beam splitter 7 for branching the surface plasmon resonance light reflected by the interaction between the reaction layer 4 formed on the metal layer 3 and the surface plasmon resonance phenomenon, and the light branched by the beam splitter 7 are detected. It is characterized by comprising a photodetector 8 that performs the above-described operation.

  Here, the introduced light introduced into the glass rod 2 passes through the inside of the glass rod 2 and is reflected by the metal layer 3 formed on the outer peripheral portion of the glass rod 2 and the first one end face.

  For example, when laser light is incident from the second end face 2b of the glass rod 2, an evanescent wave is generated at the boundary surface between the glass rod 2 and the metal layer 3, and the evanescent wave causes the boundary surface between the metal layer 3 and the reaction layer 4 to be generated. When the surface plasmon is excited and the wave number in the plane direction of the evanescent wave coincides with the wave number in the plane direction of the surface plasmon, a resonance phenomenon occurs.

  Therefore, when an antigen-antibody reaction occurs on the reaction layer 4, the dielectric constant ε changes depending on the antigen or the like attached to the reaction layer 4, and the resonance angle also changes accordingly. When this change is measured using a continuous light source such as white light, it can be observed as a wavelength shift.

  As the material of the glass rod 2, glass, quartz, plastic or the like can be applied. Further, in order to guide specific light, an impurity may be added to change the refractive index.

  The cross-sectional structure of the glass rod may be any of a circle, an ellipse, and a rectangle. A circle means a circle that can be substantially processed and created including a perfect circle.

  The material of the glass rod 2 may have a refractive index distribution or may be set to a constant refractive index. For example, the material of the glass rod 2 having a substantially circular cross-sectional structure including a perfect circle may have a refractive index distribution in the radial direction from the center of the cylinder, or set to a constant refractive index. May be.

  In the biosensor according to the first embodiment of the present invention, the introduced light introduced into the glass rod 2 causes the surface plasmon resonance phenomenon due to the interaction between substances with the reaction layer 4 formed on the metal layer 3. The surface plasmon resonance light generated and reflected on the metal layer 3 is branched by the beam splitter 7. The light branched by the beam splitter 7 is detected by the photodetector 8, and the detected data is sent to the analysis computer 9 and analyzed by the analysis computer 9.

  Furthermore, in the biosensor according to the first embodiment of the present invention, as shown in FIG. 1, a lens 6a is provided between the light source 5 and the beam splitter 7 and between the beam splitter 7 and the sensor unit 1, respectively. , 6b may be provided.

  In the biosensor according to the first embodiment of the present invention, the metal layer 3 formed on the outer peripheral portion of the glass rod 2 and the first one end face 2a is, for example, Au (gold). To do.

  The material of the metal layer 3 is not limited to gold, and silver (Ag), copper (Cu), or the like may be used. In order to improve the adhesion, chromium (Cr) or platinum (Pt) may be applied to the base, but it is not suitable for plasmon resonance, so it is important to apply it as thin as possible.

  These metal layers 3 may be stacked as a single layer, or may be combined and deposited as a multilayer film. Further, in order to easily deposit these metal layers 3, chromium (Cr) may be deposited as a base treatment. Thereby, the adhesiveness of the glass rod 2 and the metal layer 3 can be improved. Examples of the deposition method for depositing on the surface of the glass rod 2 include vapor deposition, sputtering, and CVD. The thickness of the metal layer 3 is, for example, several nanometers to several tens of nanometers, and a specific example is about 50 nm.

  Moreover, the dimension of the glass rod which comprises the sensor part 1 can also set the length several tens nm-several mm, for example, still longer. On the other hand, if the cross-sectional dimension is circular, it can be, for example, about several hundred nm to several mm, or even several cm.

  Furthermore, in the biosensor according to the first embodiment of the present invention, as shown in FIG. 1, the sensor unit 1, the light source 5, the beam splitter 7, and the photodetector 8 are optically connected in a spatial system. It is characterized by that.

  The light source 5 emits light in a wavelength band selected as appropriate, and preferably emits stable light. For example, a green light emitting diode (LED), a white LED, and a laser diode (LD: LD). Laser diode), a lamp, etc. are used. Alternatively, for example, light having a wavelength of about 400 nm to 900 nm can be used. Furthermore, near-infrared light having a wavelength of 900 nm or more or infrared light can be applied depending on the application.

  The light detector 8 detects light in at least one wavelength band among the light emitted from the light source 5, and for example, a photodiode (PD) is used.

  If the electric signal data of the photodetector 8 is input to the analysis computer 9 and processed, the change of the reaching light (surface plasmon resonance light reflected on the metal layer 3) due to the interaction between the reactant and the target substance Can be monitored in real time. That is, for example, when the sensor unit 1 in which the reaction layer 4 containing an antibody is formed is immersed in a sample solution containing an antigen, a change in the amount of binding between the antigen and the antibody is analyzed as a change in the amount of light reached with time. It can be displayed on the computer 9.

  In the biosensor according to the first embodiment of the present invention, the glass rod 2 is mainly applied to the sensor unit 1, but the cross-sectional structure is not limited to a substantially workable circle including a perfect circle. Alternatively, it may be oval or rectangular. This is because the surface plasmon resonance phenomenon can be obtained in the same manner even when the cross-sectional structure of the sensor unit 1 has any cross-sectional structure such as a circle, an ellipse, and a rectangle.

  According to the biosensor according to the first embodiment of the present invention, it is possible to detect a reaction of a sample to be measured, a concentration change, and the like using a surface plasmon resonance phenomenon with an inexpensive and simple configuration. Can be provided.

[Second Embodiment]
As shown in FIG. 2, the biosensor according to the second embodiment of the present invention is a biosensor that detects an interaction between substances by applying a surface plasmon resonance phenomenon, and includes a first end face 2a, a first one. A glass rod 2 having a two-end surface 2b and having a curved shape, a metal layer 3 formed only on the outer periphery of the glass rod 2, and a reactive substance formed on the metal layer 3 and interacting with a specific substance The sensor part 1 which has the reaction layer 4 containing is provided.

  Furthermore, the biosensor according to the second embodiment of the present invention includes a light source 5 that introduces light into the first end surface 2a of the glass rod 2 and an introduction introduced into the glass rod 2, as shown in FIG. A light detector 8 for detecting light and a reaction layer 4 formed on the metal layer 3 and a plasmon resonance light reflected by a substance-to-material interaction due to a surface plasmon resonance phenomenon from the second end surface 2b of the glass rod 2; It is characterized by providing.

  Here, the introduced light introduced into the glass rod 2 passes through the inside of the glass rod 2 and is reflected by the metal layer 3 formed on the outer peripheral portion of the glass rod 2.

  When laser light is incident from the first end face 2 a of the glass rod 2, an evanescent wave is generated at the interface between the glass rod 2 and the metal layer 3, and the surface plasmon is generated at the interface between the metal layer 3 and the reaction layer 4 by the evanescent wave. Occurs as a resonance phenomenon when the wave number in the plane direction of the evanescent wave coincides with the wave number in the plane direction of the surface plasmon.

  Therefore, when an antigen-antibody reaction occurs on the reaction layer 4, the dielectric constant ε changes depending on the antigen or the like attached to the reaction layer 4, and the resonance angle also changes accordingly. When this change is measured using a continuous light source such as white light, it can be observed as a wavelength shift.

  In the biosensor according to the second embodiment of the present invention, as shown in FIG. 2, the introduced light introduced into the glass rod 2 is exchanged between substances with the reaction layer 4 formed on the metal layer 3. The surface plasmon resonance phenomenon is caused by the action, and the surface plasmon resonance light reflected on the metal layer 3 is derived from the second one end face 2b of the glass rod 2. Here, the derived light is detected by the photodetector 8 as shown in FIG. 2, and the detected data is sent to the analysis computer 9 (not shown) as in the first embodiment. Analysis is performed in the analysis computer 9.

  Furthermore, in the biosensor according to the second embodiment of the present invention, as shown in FIG. 2, between the light source 5 and the first piece end surface 2 a of the glass rod 2 and the second piece end surface 2 b of the glass rod 2. Lenses 6a and 6b may be provided between the optical detector 8 and the photodetector 8, respectively.

  In the biosensor according to the second embodiment of the present invention, the metal layer 3 formed on the outer peripheral portion of the glass rod 2 is, for example, Au (gold).

  The material of the metal layer 3 is not limited to gold, and silver (Ag), copper (Cu), or the like may be used. In order to improve the adhesion, chromium (Cr) or platinum (Pt) may be applied to the base, but it is not suitable for plasmon resonance, so it is important to apply it as thin as possible.

  The thickness of the metal layer 3 is, for example, several nanometers to several tens of nanometers, and a specific example is about 50 nm.

  Moreover, the dimension of the curved-shaped glass rod which comprises the sensor part 1 can also set the length several tens nm-several mm, for example, still longer. On the other hand, if the cross-sectional dimension is circular, it can be, for example, about several hundred nm to several mm, or even several cm.

  These metal layers 3 may be stacked as a single layer, or may be combined and deposited as a multilayer film. Further, in order to easily deposit these metal layers 3, chromium (Cr) may be deposited as a base treatment. Thereby, the adhesiveness of the glass rod 2 and the metal layer 3 can be improved. Examples of the deposition method for depositing on the surface of the glass rod 2 include vapor deposition, sputtering, and CVD.

  Furthermore, in the biosensor according to the second embodiment of the present invention, as shown in FIG. 2, the sensor unit 1, the light source 5, and the photodetector 8 are optically connected in a spatial system. And

  Furthermore, in the biosensor according to the second embodiment of the present invention, as shown in FIG. 2, the vicinity of the first piece end surface 2a and the vicinity of the second piece end surface 2b of the curved glass rod 2 are accommodated. The positioning substrate member 10 having a curved groove equal to the curvature radius of the curved shape of the glass rod 2 may be provided. As a material of the positioning substrate member 10, a material that can be easily processed, such as a silicon substrate or a glass substrate, can be used.

  In the biosensor according to the second embodiment of the present invention, as shown in FIG. 2, in the sensor unit 1, a groove is previously provided so that the curved glass rod 2 is fixed with a predetermined curvature. A light source 5 that includes a substrate member 10 and further enters light into one end of the sensor unit 1, and a photodetector 8 that is incident from one end of the sensor unit 1, passes through the inside, and detects the passed light. Consists of including.

  In the biosensor according to the second embodiment of the present invention, the reaction layer 4 formed on the metal layer 3 is exposed to the outside of the positioning substrate member 10 as shown in FIG. It is characterized by.

  As the material of the glass rod 2, glass, quartz, plastic or the like can be applied. Further, in order to guide specific light, an impurity may be added to change the refractive index.

  The cross-sectional structure of the glass rod may be any of a circle, an ellipse, and a rectangle. A circle means a circle that can be substantially processed and created including a perfect circle.

  The material of the glass rod 2 may have a refractive index distribution or may be set to a constant refractive index. For example, the material of the glass rod 2 having a substantially circular cross-sectional structure including a perfect circle may have a refractive index distribution in the radial direction from the center of the cylinder, or set to a constant refractive index. May be.

  According to the biosensor according to the second embodiment of the invention, the curved glass rod 2 of the sensor unit 1 that resonates with the surface plasmon is immersed in the sample to be measured (liquid) or directly measured on the metal layer 3. When light is incident from the first one end surface 2a of the glass rod 2 and the transmitted light intensity emitted from the second one end surface 2b is measured in a state where the sample is in contact with or placed on the glass rod 2, the light propagating through the glass rod 2 is measured. An evanescent wave is generated at the boundary surface between the rod 2 and the metal layer 3, and surface plasmons are excited at the boundary surface between the metal layer 3 and the sample to be measured by its action. Due to the resonance phenomenon that occurs when the wave number in the plane direction of the evanescent wave coincides with the wave number in the plane direction of the surface plasmon due to the concentration of the sample to be measured and the antigen-antibody reaction, the transmission emitted from the second end face 2b of the glass rod 2 A characteristic in which the light intensity rapidly attenuates can be obtained.

  Further, in the biosensor according to the second embodiment of the present invention, the sensor unit 1 having the glass rod 2 which is bent at a predetermined radius of curvature and is miniaturized is configured. Therefore, the surface plasmon resonance sensor is It is possible to easily reduce the size of the sensor and to increase the length of the reflective portion of the surface where plasmon resonance can be obtained in a small space. As a result, a highly sensitive sensor can be obtained.

  An example of a method for forming the glass rod 2 which is bent with such a predetermined radius of curvature and is miniaturized will be described below.

  A glass rod 2 having a predetermined length is prepared, the outer periphery is softened and melted with a heat source (for example, a gas burner), and bent into a U-shape. At this time, it is desirable that the bending angle of the U-shaped curved portion be bent at an angle centered at 180 °, for example.

  Next, the metal layer 3 is deposited with a thickness of about 100 nm or less on the surface of the glass rod 2 bent into a U-shape using, for example, a vacuum evaporation method. The deposition method is not limited to the vacuum evaporation method, and a deposition method such as a sputtering method or a CVD method may be used. Through the above process, the bent glass rod 2 shown in FIG. 2 can be obtained.

  As another forming method, an optical waveguide can be used. In this case, a material to be the glass rod 2 is formed in a layer shape on a flat glass plate, and this layer is covered with, for example, a U-shaped mask, and the other layers are removed by etching to form a U-shape. A glass rod 2 having a curved shape can be formed by forming a glass rod having a shape and forming a metal layer 3 made of a conductive material on the glass rod. In this case, the cross-sectional shape of the glass rod 2 is rectangular, but the surface plasmon resonance sensor is small because the sensor unit 1 having the glass rod 2 that is bent at a predetermined radius of curvature and is downsized is configured. The sensor can be easily formed by increasing the length of the reflective portion of the surface where plasmon resonance can be obtained in a small space, and as a result, a highly sensitive sensor can be obtained with a circular cross section. This is the same as the case where the elliptical glass rod 2 is used.

  Further, the surface plasmon resonance phenomenon can be obtained in the same manner even when the cross-sectional structure of the sensor unit 1 has a cross-sectional structure of any shape such as a circle, an ellipse, and a rectangle.

  The light source 5 emits light in a wavelength band selected as appropriate, and preferably emits light stably. For example, a green LED, a white LED, an LD, a lamp, or the like is used. Alternatively, for example, light having a wavelength of about 400 nm to 900 nm can be used. Furthermore, near-infrared light having a wavelength of 900 nm or more or infrared light can be applied depending on the application.

  The photodetector 8 detects light in at least one wavelength band among the light emitted from the light source 5, and for example, a PD is used.

  As in the first embodiment, if the electrical signal data of the photodetector 8 is input to the computer 9 for analysis and signal processing is performed, changes in the reaching light accompanying the interaction between the reactant and the target substance are monitored in real time. can do. That is, for example, when the sensor unit 1 in which the reaction layer 4 containing an antibody is formed is immersed in a sample solution containing an antigen, a change in the amount of binding between the antigen and the antibody is analyzed as a change in the amount of light reached with time. It can be displayed on the computer 9.

  An example of a measurement result using the biosensor according to the second embodiment of the present invention is schematically represented as shown in FIG.

  In the measurement of the interaction between substances using the biosensor according to the second embodiment of the present invention, the sensor unit 1 having the reaction layer 4 formed on the surface is immersed in the sample solution to be measured, At this time, the reaching light (surface plasmon resonance light) on the second end face 2 b of the glass rod 2 is detected by the photodetector 8.

  That is, the reaching light in a state where the sensor unit 1 is not immersed in the sample solution is detected in advance, and the reaching light after the sensor unit 1 is immersed in the sample solution is detected, and changes in these reaching lights are evaluated. To do. At this time, if the sample solution contains a specific substance (target substance) that interacts with the reactive substance contained in the reaction layer 4 of the sensor unit 1, the interaction causes the arrival as shown in FIG. Since the amount of light changes, the presence / absence or amount of the target substance in the sample solution can be measured from the change in the amount of light reached.

  According to the biosensor according to the second embodiment of the present invention, it is not necessary to use a prism such as a beam splitter, and the number of components can be reduced as compared with the first embodiment.

  According to the biosensor according to the second embodiment of the present invention, a biosensor that can detect a reaction of a sample to be measured, a concentration change, and the like using a surface plasmon resonance phenomenon with an inexpensive and simple configuration. Can be provided.

[Other embodiments]
As described above, the present invention has been described according to the first to second embodiments. However, it should not be understood that the description and drawings constituting a part of this disclosure limit the present invention. From this disclosure, various alternative embodiments, examples and operational techniques will be apparent to those skilled in the art.

  As described above, the present invention naturally includes various embodiments that are not described herein. Therefore, the technical scope of the present invention is defined only by the invention specifying matters according to the scope of claims reasonable from the above description.

The typical block diagram of the biosensor which concerns on the 1st Embodiment of this invention. The typical block diagram of the biosensor which concerns on the 2nd Embodiment of this invention. The typical characteristic view which shows an example of the measurement result using the biosensor which concerns on the 2nd Embodiment of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Sensor part 2 ... Glass rod 2a ... 1st one end surface 2b of a glass rod ... 2nd one end surface 3 of a glass rod ... Metal layer 4 ... Reaction layer 5 ... Light source 6a, 6b ... Lens 7 ... Beam splitter 8 ... Light detection Instrument 9 ... Analytical computer 10 ... Positioning substrate member

Claims (9)

A biosensor that detects the interaction between substances by applying the surface plasmon resonance phenomenon,
A glass rod,
A metal layer formed on the outer periphery of the glass rod and the first end surface;
A biosensor comprising: a sensor unit having a reaction layer formed on the metal layer and containing a reactive substance that interacts with a specific substance. The biosensor according to claim 1, wherein
A light source for introducing light into the second end surface of the glass rod;
A beam splitter for branching the introduced light introduced into the glass rod, and the surface plasmon resonance light reflected by the interaction between the reaction layer formed on the metal layer and the substance due to the surface plasmon resonance phenomenon;
A biosensor further comprising: a photodetector that detects light branched in the beam splitter. The biosensor according to claim 1 or 2,
The biosensor according to claim 1, wherein the metal layer formed on the outer peripheral portion and one end face of the glass rod is Au (gold). The biosensor according to claim 2, wherein
The biosensor, wherein the sensor unit, the light source, the beam splitter, and the photodetector are optically connected in a spatial system. A biosensor that detects the interaction between substances by applying the surface plasmon resonance phenomenon,
A glass rod having a first end surface, a second end surface, and having a curved shape;
A metal layer formed only on the outer periphery of the glass rod;
A biosensor comprising: a sensor unit having a reaction layer formed on the metal layer and containing a reactive substance that interacts with a specific substance. The biosensor according to claim 5, wherein
A light source for introducing light into the first end surface of the glass rod;
The introduced light introduced into the glass rod, and the plasmon resonance light reflected by the interaction between the reaction layer formed on the metal layer and the substance due to the surface plasmon resonance phenomenon from the second one end face of the glass rod A biosensor further comprising: a photodetector for detection. The biosensor according to claim 5 or 6,
The biosensor according to claim 1, wherein the metal layer formed on the outer periphery of the glass rod is Au (gold). The biosensor according to claim 6, wherein
The biosensor, wherein the sensor unit, the light source, and the photodetector are optically connected in a spatial system. The biosensor according to any one of claims 5 to 8,
A substrate member that accommodates the vicinity of the first piece end face and the vicinity of the second piece end face of the glass rod having a curved shape, and has a curved groove equal to the radius of curvature of the curved shape of the glass rod;
The biosensor according to claim 1, wherein the reaction layer formed on the metal layer is exposed to the outside of the substrate member.

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