JP2008001658A - Cosmetic composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To enable a cosmetic composition having industrially stable quality to produce economically, as giving various kinds of excellent functions by adding the extracts from various kinds of natural substances, as decreasing the problems caused by using natural products. <P>SOLUTION: The cosmetic composition is prepared by using extracts from lignite. Lignite is treated with water and/or organic solvent at 60 to 374°C for 1 second to 24 hours under pressure of 0.1 to 30 MPa to obtain lignite extract. This cosmetic includes the lignite extract in an amount of 0.001 to 100 wt.% based on the total amount. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a cosmetic composition. Specifically, the present invention is directed to a cosmetic composition in which an active ingredient extracted from a natural product is blended to improve a skin whitening action, an anti-aging action, and the like.

It is well known that, by incorporating an active ingredient extracted from a natural product such as a plant as an active ingredient of a cosmetic composition, a useful function that cannot be obtained with a chemically synthesized substance can be exhibited. .
As natural extract components useful for cosmetic compositions, for example, plant-derived components such as aloe, licorice and camellia, and animal-derived components such as placenta extract and silk protein are well known. In addition to animals and plants, mud, sea salt, hot spring water and the like are said to contain useful ingredients in the cosmetic composition.
For example, Patent Document 1 discloses a cosmetic technology in which Mole hot spring water that springs from a specific spring source and an extract extracted from a white crane reishi of a plant that is a raw material for herbal medicine.
JP 2000-239146 A

The active ingredients obtained from animals, plants and other natural products blended in the cosmetic composition as described above are not a single compound or substance, but a combination of a plurality of substances whose molecular structures are unknown. Many. In addition, the combination of components obtained may vary depending on the plant production area, growth stage, and other raw material collection conditions, and the intended function may vary.
However, in the case of industrially produced cosmetics, it is not preferable that the function and performance vary greatly depending on the raw materials used. Biological materials such as animals and plants have a drawback that such variations are likely to occur. There are many variations in mud and hot spring water depending on the collection location.

In addition, components derived from biological raw materials may contain pathogenic bacteria, viruses and other harmful substances peculiar to biological raw materials, and a processing step for removing these harmful substances is required.
The technique of Patent Document 1 also has the same problem as described above for plant raw materials. The content of hot spring water can vary greatly depending on the rainy season and dry season.
An object of the present invention is to provide various excellent functions to a cosmetic composition by using an extract from a natural product, and reduce the problems of using a natural product, thereby producing an industrially stable quality. It is to make it possible to economically produce high performance cosmetic compositions.

The cosmetic composition according to the present invention comprises a product extracted from lignite, that is, a lignite extract.
A specific configuration will be described in detail below.
〔lignite〕
Although lignite is included in the concept of coal in a broad sense, it is technically distinct from general coal. In the process of ancient trees and other plants being coalified in the ground, coalization is not sufficiently advanced, and plant-derived organic substances remain relatively abundant. Among coals, coalification has not progressed more than lignite and bituminous coal, and the calorific value as fuel is small.
If it is a substance contained in such a lignite concept, its mining site and mining layer are not limited. For example, lignite produced in Hokkaido and Obihiro regions of Japan can be used.

[Extraction]
In order to obtain a lignite extract from lignite, extraction techniques from general biological materials and mineral materials can be applied.
Extraction methods include water extraction technology using water as an extraction medium, organic solvent extraction technology using an organic solvent as an extraction medium, heat extraction with heating, pressure extraction under pressure, vacuum extraction under vacuum, acid extraction, alkali Various extraction techniques such as extraction are known. These extraction techniques can be used alone or in combination.
The method of using water as the extraction medium is particularly preferable from the viewpoint of easy handling.

When an organic solvent is used as the extraction medium, it is not particularly limited. For example, alcohols such as ethanol, 1,3-butylene glycol, methanol, and propanol, fats such as squalene, jojoba oil, hexane, heptane, and liquid paraffin Aromatic hydrocarbons such as benzene and toluene, esters such as ethyl acetate, ethers such as dimethyl ether and methyl ethyl ether, ketones such as acetone, and the like. These organic solvents can be used alone or in admixture of two or more, or in combination with water.
It is effective to extract at an extraction temperature of 60 to 374 ° C., an extraction time of 1 second to 24 hours, and an extraction pressure of 0.1 to 30 MPa. The extraction temperature is preferably 120 to 280 ° C. The extraction time is 1 second to 3 hours. The extraction pressure is 0.1 to 22 MPa. In the case of lignite, since it is difficult to extract active ingredients at low temperatures and low pressures compared to ordinary animal and plant materials, it is preferable to spend a long time at high temperatures and high pressures. Moreover, even if a lignite extract is exposed to a relatively high temperature and high pressure for a long time, it does not easily cause a problem that its function is degraded due to decomposition or alteration. However, the higher the temperature or pressure, the easier it is to extract special components that are difficult to extract by normal extraction, so it is necessary to confirm that no harmful components are contained in the extract.

As the extraction device, an autoclave device that can easily maintain a high temperature / high pressure environment can be used.
Prior to the extraction step, it is possible to perform a process of removing unnecessary substances such as dust or washing from the lignite raw material. In addition to water, acid, alkali, etc. can be used for the cleaning liquid.
The lignite as the raw material for extraction can be extracted as a lump mined from the ground, but if it is pulverized into fine granules, it can be extracted easily. Specifically, it can be pulverized to an average particle size of 10 mm or less. Preferably, extraction is performed with an average particle size of 0.5 to 5 mm.
[Lignite extract]
By performing the extraction process, an extract containing an extract from lignite is obtained. The extraction liquid can be subjected to various post-treatments such as purification as in the case of a normal extraction technique.

When an organic solvent is used as the extraction medium, the organic solvent can be replaced with another organic solvent or water.
The extract can be filtered or centrifuged to remove unnecessary solids and impurities. A pH adjustment treatment can also be performed.
In this way, a lignite extract liquid containing the lignite extract in the extraction medium is obtained.
[Cosmetic composition]
Basically, the same technique as that for a cosmetic composition containing various usual extraction components can be applied.
The cosmetic composition includes not only the final cosmetic product but also an intermediate product for producing the cosmetic product.

The lignite extract has excellent tyrosinase inhibitory activity and melanin synthesis inhibitory activity as functions required for the cosmetic composition. Antioxidant activity such as SOD (superoxide dismase) -like activity, hydroxy radical scavenging activity, and hyaluronidase inhibitory activity are also excellent. It is useful to blend in cosmetics that require these functions.
These functions are briefly described below.
<Tyrosinase inhibitory activity>
Melanin, which is harmful to skin whitening in the body, is synthesized by being metabolized to DOPA and DOPAquinone by tyrosinase using tyrosine, which is one of amino acids, as a substrate. This initial reaction is the rate-limiting step of melanin synthesis. Thus, tyrosinase acts in the process where tyrosine is oxidized and synthesized into melanin. When tyrosinase is activated, it causes problems such as spots and pigmentation on the skin. If the activity of tyrosinase can be inhibited, it is effective for whitening the skin.

The tyrosinase inhibitory activity can be evaluated by the value of the concentration necessary to inhibit the enzyme activity by 50%: IC50 (mg / ml). The smaller this value, the better the tyrosinase inhibitory activity, the synthesis of melanin pigment caused by tyrosinase is suppressed, and the whitening effect is evaluated to be excellent.
<Melanin synthesis inhibitory activity>
Melanin pigment synthesis occurs when multiple elements are combined together. As the element, in addition to the above-mentioned tyrosinase, there are active oxygen that stimulates melanin pigment cells. It is effective to comprehensively evaluate all the factors involved in the synthesis of melanin pigments.

Therefore, the melanin synthesis inhibitory activity is related to the tyrosinase inhibitory activity described above, but is evaluated including the synthesis of melanin pigments caused by causes other than tyrosinase.
The melanin synthesis inhibitory activity can also be evaluated by the value of the concentration required to inhibit the activity by 50%: IC50 (mg / ml). It is evaluated that the smaller this value, the higher the melanin synthesis inhibitory activity and the better the skin whitening effect.
<SOD-like activity>
One active oxygen is superoxide. This superoxide causes the following .OH, which is a stronger active oxygen. SOD (superoxide dismutase) is an enzyme having a function of eliminating superoxide.

However, it is difficult to artificially produce the SOD itself. It is thermally unstable, easily deactivated, difficult to purify, and expensive. The SOD-like activity test is evaluated by the amount of SOD units [SODunits / g] exhibiting an equivalent activity function for various substances. The larger this value, the higher the SOD-like activity, making the active oxygen harmless and suppressing aging due to skin oxidation.
<OH scavenging activity>
In the body or body surface, a substance called aging and carcinogenic action called active oxygen is constantly generated. OH (hydroxy radical) is an active oxygen that is known to be very harmful to the skin among the active oxygen generated in the body.・ If OH can be eliminated, internal oxidation can be prevented. Skin aging can be suppressed.

OH scavenging activity can also be evaluated by the value of the concentration necessary to inhibit OH activity by 50%: IC50 (mg / ml). The smaller this value, the more the .OH will be erased, and the .OH elimination activity will be higher, indicating that it is effective for preventing skin aging and improving protection.
<Hyaluronidase inhibitory activity>
Hyaluronic acid is one of the components involved in skin moisture, and the enzyme that decomposes it is hyaluronidase. If hyaluronidase can be inhibited, skin aging can be suppressed.
Hyaluronidase inhibitory activity can also be evaluated by the concentration required to inhibit hyaluronidase activity by 50%: IC50 (mg / ml). The smaller this value, the higher the hyaluronidase inhibitory activity, indicating that it is more effective in preventing skin aging and improving protection.

<Collagenase inhibitory activity>
Collagen is a protein abundant in the skin, and the enzyme that breaks it down is collagenase. If the collagenase activity can be inhibited, skin aging such as wrinkles and tarmi can be suppressed.
Collagenase inhibitory activity can also be evaluated by the concentration value required to inhibit collagenase activity by 50%: IC50 (mg / ml). The smaller this value, the higher the collagenase inhibitory activity, indicating that it is more effective in preventing skin aging and improving protection.
In the cosmetic composition, the lignite extract can be blended at 0.001 to 100% by weight of the total amount. Preferably it is 10 to 100 weight%. In addition to the lignite extract, water and other organic solvents can also be included, and the lignite extract liquid obtained by the extraction operation can be directly blended into the cosmetic composition without removing the extraction solvent. When the cosmetic composition is a cosmetic, various components blended in the cosmetic are also included.

Specifically, the cosmetic composition of the present invention is exemplified in the following (1) to (44) as long as the effects of the present invention are not impaired, if necessary, in addition to the essential lignite extract. Ingredients and additives may be arbitrarily selected and used in combination. Thereby, an additive or synergistic improvement of the effects of the present invention or other cosmetic effects can be expected. In addition, although content of the component and additive illustrated to following (1)-(44) is not specifically limited, Usually, it is preferable to set it as the range of 0.0001 to 50 weight% in a composition, respectively.
(1) Various fats and oils: avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange raffer oil, sesame oil, cacao butter, chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, cucumber nut oil, safflower Oil, shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil, egg yolk oil, palm oil, palm kernel oil, owl, Coconut oil, beef tallow, lard, squalene, squalane, pristane or hydrogenated products of these oils (hardened oil, etc.)

(2) waxes; beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax and the like.
(3) Mineral oil; liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, micro-crystal wax and the like.
(4) Fatty acids: lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, linoleic acid, linolenic acid, docosahexaenoic acid, eicosapentaenoic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin Synthetic fatty acids such as natural fatty acids such as fatty acids, isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid and isopentanoic acid.

(5) Alcohols; synthesis of natural alcohols such as ethanol, isopropanol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, phytosterol, phenoxyethanol, 2-hexyldecanol, isostearyl alcohol, 2-octyldodecanol, etc. alcohol.
(6) Polyhydric alcohols; ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol, polypropylene Glycol, 1,3-butylene glycol, pentyl glycol, glycerin, pentaerythritol, threitol, arabitol, xylitol, ribitol, galactitol, sorbitol, mannitol, lactitol, maltitol and the like.

(7) Esters: isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyldodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, myristyl lactate Diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.
(8) Metal soaps: aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

  (9) Gum, sugars or water-soluble polymer compounds; gum arabic, benzoin rubber, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum, carob gum, quinseed, agar, casein, lactose, fructose, sucrose or esters thereof , Trehalose or derivatives thereof, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or chitosan, hydroxyalkyl (C2-C4) chitin or chitosan to which alkylene (C2-C4) oxide such as ethylene oxide is added, low molecular weight Chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid or salt thereof, hyaluronic acid or salt thereof, chondroitin sulfate or salt thereof, heparin, ethylcellulose, methyl Rulose, carboxymethyl cellulose, carboxyethyl cellulose, sodium carboxyethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, nitrocellulose, crystalline cellulose, polyvinyl alcohol, polyvinyl methyl ether, polyvinyl pyrrolidone, polyvinyl methacrylate, polyacrylate, polyethylene oxide, polypropylene oxide, etc. Polyalkylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, polyethyleneimine and the like.

  (10) Surfactant; anionic surfactant (alkyl carboxylate, alkyl sulfonate, alkyl sulfate ester salt, alkyl phosphate ester salt), cationic surfactant (alkyl amine salt, alkyl quaternary ammonium salt), Amphoteric surfactant: Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (Ether type nonionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (Natural surfactants, protein hydrolyzate derivatives, polymer surfactants, surfactants containing titanium and silicon, Carbon-based surface active agent) and the like.

  (11) Various vitamins; vitamin A group: retinol, retinal (vitamin A1), dehydroretinal (vitamin A2), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (vitamin B1) , Riboflavin (vitamin B2), pyridoxine (vitamin B6), cyanocobalamin (vitamin B12), folic acid, nicotinic acids, pantothenic acids, biotins, choline, inositols, vitamin C group: vitamin C or derivatives thereof, vitamin D group: Ergocalciferol (vitamin D2), cholecalciferol (vitamin D3), dihydrotaxosterol, vitamin E group: vitamin E or derivatives thereof, ubiquinones, vitamin K group: phytonadione (vitamin K1), menaquinone (vitamin K2) Menadione (vitamin K3), menadiol (vitamin K4), other essential fatty acids (vitamin F), carnitine, ferulic acid, .gamma.-oryzanol, orotic acid, vitamin P such (rutin, eriocitrin, hesperidin), vitamin U and the like.

(12) Various amino acids: valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxy Amino acid derivatives such as lysine, arginine, ornithine, histidine, sulfates, phosphates, nitrates, citrates, or pyrrolidone carboxylic acids.
(13) Various additives derived from plant or animal raw materials; specific raw materials used as raw materials include i) to v) below.

i) As a plant (herbal medicine), for example, almond (a peach), eye (Aiba), Aokazura (Seifu Fuji), Aoki (Aoki), Aogiri or Kenashia Ogiri (Ao Tung), Aotsu Rafuji (Kyotoen), Ao Tenma, Acacia, Red clover (red hempen), redcurrant “fruit”, red clover, red grape, red vine (logwood), red mulberry (red bud), red cypress or red pine or red radish (red root), red radish or red fox (ground yellow), red eel (red fox) , Aki larch, Akinire (crusted skin), Aguay Guassu, Aguahe (Omiten coconut palm), Akebi (Kitsu), Asa (Asako Jin), Maruba morning glory or morning glory (Check cow), Hydrangea (hydrangea), Ashitaba (Tomorrow) , Azuki (red bean), Asunaro, Acerola, Asenyaku (Asen Yaku), Chilla (shokuyokanna), anise, abiu, abiurana, rape, abemaki "fruit", avocado, ama, acha (Amacha), amacharu, amadokoro (bamboo bamboo), amana (kojien), amaranth (hyu, hagateou, himegeoto) , Garlic, Japanese cedar, Hosoaogeito, Aogaeto, Haribi, Aozulan, Amaranthus hippochondriax, Amigasayuri or Baimo (shellfish), Aristolomeria (Urizuisen), Alcana (alcanna), Algarrobo (cave), Altea, Altea, Altea Or Katsudaidai (Suzuku), Aloe (Amber), Aloe Vera, Angelica, Apricot or Honzu (Anzujin), Ansokkou (benzoic incense), Yellow support, Igakouzorina (Gallhead), Ikariso or Yachima Taikiso (Japanese Yakukaku), Rabbit (Ginseng Grass), Japanese Knotweed (Tiger root), Yew (1st place), Strawberry, Fig (No flower "fruit, leaf"), Ichihatsu (first), Ichibichi (winter lion), Ichiyakuso (One medicinal herb), ginkgo (Ginkgo "seed, leaves"), Japanese beetle (distant), locust bean, Inazuna (tailexi), Inubiyu (Honagainubiyu), rice "seed, seed coat", Inondo "seed", Ibukizasou, nettle, Iran Iran, Iwa Tobacco (Iwabuchi), Iwahiba or Iwamatsu (rolling mushrooms), common bean, oolong tea, fennel (mud), wheat (chibusanoki), Ukiyagara (three ridges), Uguisukagura "fruit", Japanese yellow turf, turmeric (depressed gold), Duckweed, Usbasaishin or Keirinsaishin or Oshusaishin (fine Spicy), usbenia mushroom, usbenitachaoi mushroom, phalaenopsis, weevil (summer hay), udo or shishiudo (animated, solitary, tangdoku), unyadegato (unkalia, cat's claw), uba tea, ume (meal plum "seed, pulp"), Vulgaris, rumors (Prickly berries), Satsuma mandarin (Chen), Unboku, Estragon, Ezoukogi (蝦 夷 五 加), Ezo lily of the valley, Echinashi (Horaxa barrengiku), Enishida, Enokitake (榎 茸), Ebisugusa or Cassia torakan Elderberry “fruit”, Elemi, Elingi or Pre-Lotus Elinji, Enju (Spirit, Satsuma Rice), Pea, Ogi or Kibana-Ogi (Yellow), Naruko Yuri or Kagura-mabanaru Yuri (Yellow), Ohisano Yuki or Sasanoyuki, Uren (Huang Ren), Ogata Houken, Okarasuuri (Karokon), Oguruma (Civilian incense), Ootsu Rafuji (Preventive Self), Psyllium (car front child, car front grass), Japanese thistle (Zhengzhou Leakage, Bald Eagle), Toucan Whale (lion) , Tossed cactus, bigeye okera or okera (white birch), giant crape myrtle (banaba), giant fruit bush (fruit), great squirrel (cereals), omia catechu, great hawthorn or hawthorn (mountain barley), coconut palm, barley, barley, barley , Okazeri (snake bed), okra "fruit", oguruma (turnover), ogururi higotai (Zhengzhou leakage, indigo sashimi), ginseng or ginseng (carrot), hypericum or barley, hypericum pertussis (brother cut grass), Odorikosou (continuous), Onamomi (蒼 ear child) Onigurumi, Onidokoro or Tokoro or Nagadokoro (Hikai), Onino Yagara (Tempa), Onyuri or Sasayuri or Hakatayuri (Yuri), Onoelan, Ononis, Halibut (Flying Crab), Ominaeshi (Defeated Soy Sauce), Oyuko, Dutch Karashi (Cresson), Dutch jellies, Dutch biloba, Dutch honey bees, olives “fruit, seeds, leaves”, oregano, orange “fruits, pericarp”, silkworms (ripening yellow), cacao “fruits, pericarps, seeds”, oysters (persimmon “leaves”) , Kagikazura (Katsufuji), Kakidooshi or Kantorisou (lotus), Kakiran, Gaku hydrangea, Cassia, Casino oyster (fruit berries), Gadju (莪 朮), Kashiwa (Kashiwagi, Kashiwa), Cascarilla, Kaka Scala Sagrada, Gypsophila, Kasensou, Crab (Kinshato), Kaniwa, Carnation, Kano Kiso (Valgara Root), Hippopotamus, Birch or Weeping Birch (White Birch), Gerbera, Pumpkin, Kapokki "Seeds", Japanese Clover (Powder), Gama (Yellow Yellow), Chamomile or Roman Chamomile, Kamiyatsude (Grass), Cam Come (Camo duck) ), Karagowa or Yamaguwa (mulberry camellia), Collar, Carax salmon man, Calla cucumber or Chinese cabbage uri (Wang 瓜), Callas bishak (half-summer), Oats, Garana “seed”, Karahoo (Koh-Pak), Karaya, Garyu, Karin (wooden), Garcinia, Kawamidori, Kawara Psycho (Deling rape, tranquil grass), Kawajisha, Kawaratake, Kawaranadeshiko (stone bamboo) or Ezokawara Nadeshiko (Soba, buckwheat), Kawaran ginseng (blue potato), Kawara mugwort (茵 Chen 蒿), Kansui (sweet) End), licorice, licorice, candelilla, kanto Kanna, kanran, raspberry (Ezo strawberry, Dutch strawberry, shrimp strawberry, navashiro strawberry, maple strawberry, European raspberry), kiwi "fruit, leaf", kikarasuuri (guro-root), kyo-kyo (flower bellflower, bellflower root), chrysanthemum (chrysanthemum flower, shrimp daisy, Korean chrysanthemum), Kikutanikuiku, Kisagi (Kashimi), Shigegishi (Sheepbone), Pheasant (Kashimi), Kizuta, Kidachi Chie, Kachidachiha, Kina, Kinanoki (Shinkona, Akinakiki), Kinua (Kinoa), Yellowfin, Yellowfin, Gymnema sylvestre, Kimenkaku, cabbage, cabbage "unripe fruit", caraway, cucumber, gyory (Nishikawa willow, taiyanagi), Kiraja saponaria, kiraya, Kiranso (Kinju hay), kumquat "fruit", kinkiju, ginsekai, Kimbusen, betel, Nymphalus, quinlan, gin orchid, goldfish, guapeba vermelha, guava "fruit", guayule, quercus infectria (garlic), kukui nuts, kugenumaran, wolfberry (bamboo shoots, coconut leaves, bamboo leaves, ground bark), Kuzusugi Kazura (Tenmon Winter), Kuzu (Kazunori), camphor, gooseberry “fruit”, carrot carrot (yellow flower moth), cutty river, gardenia (mountain lion), kunugi (sawflower), Kupuas, Kubeba, Kumazasa, Kumatsu ヅ ra (horse whip) Grass), Clara (bitter), cranberry “fruit”, chestnut “seed, fruit, astringent skin”, curcumigo latifolia “fruit”, grapefruit “fruit and leaf”, buckthorn, black-headed mochi (rescue), kamebahikokoshi or kurobanahikokoshi or Hikiokoshi (prolonged life grass), cloves (Ding Child, Dingka), Gumbainazuna (Sekimei, Sekimeishi), Keigai (Kashiwa, Aoiho), Keito (Couple flower, chicken clover), Gekkavidin, Gecko (Laurel), Kenashi Sartorii rose (Tsumago, Yamakage), Gentiana, Gennoshouko ( Old lady's grass), Kemponashi (Kugushi), Kishumikan (Koji, Tachibana, Obenimikan, Fukurikukan, Sagamikoji, Ponkan, Santara (Tachibana)), Koushinbara (Monthly Flower), Kouseihakka, Kouzo "fruit", Kocha (tea) ), Kohone (river bones), koh hong (Tsubakimoto, Karamotomoto), Koryang, Kouryo Kyo (Koryo), cilantro "fruit", kohoren (huoyuren), koganebana (yellow gon), cowberry (Etsutachibana), coconut "fruit" ”, Goshuyu, Gosho Strawberry, Pepper (Hu) ), Cosmos, copaiba balsam, coffee “seed, leaves”, kobushi or magnolia (hot pepper), burdock (beef bowl, beef eggplant), kobotan, sesame (sesame seeds), sesame seedling (genus ginseng), trash (tasty), sanekazura or binankazura Or pine bush, wheat (wheat), rice or rice bran “red cocoon, white birch”, rice oil, cola acuminata “seed”, cola bella “seed”, coleus forskori, colony llya, fenugreek “fruit”, colombo, condurango, Comb, Konjac, Comfrey (Changzhou), Sisal (Sisal Asa), Sai Hairan, Sakaneran (Ezosakaneran), Saximara buoy or cinnabar bud or Chinese ginseng (weiken), Sakura (Oshima cherry, Yamazakura, Oyamazakura, Edhigakusa, Edible cherry, Miyazakura, Seo Yoshino, Takane cherry, Kasumi cherry, Kohigan, Sato cherry, Kanzakura (leaf, flower, fruit, bark (cherry bark)), cherries, pomegranate, Sasa, Sasabagin orchid, Sazanka, Sadamodaka (Sweet potato), Sweet potato, Sugar cane, Sugar beet, Sun pea Acid sour), saffron (banban flower, west safflower), sapodilla, sapotea mariyo, pomelo "fruit", savonso, salmon berry "fruit", salamander, arabic sardine (salmon indigo head), salvia (sage), Sagaki (mountain radish), Sawagurumi (mountain walnuts), Sangaku cactus, Sangre degrad (croton), Sangikuyosou, Sansino carrot (Thirty-seven ginseng), Sanshuyu (mountain), Sansho (mountain), Sandscon (mountain) Root), shea (carite), cyanoki "fruit", shi Bamboo shoots, Shion, Shikakumame, Dikitaris, Shikunshi (Principal), Perilla or Aojiso or Chilemenzo or Katamenjizo (Shiyoha, Shiso), Rosewood, Linden, Syringa sycaensis, Reindeer (Ream), Shimeji , Shakashimeji, Hatake-shimeji, Oshiroi-shimeji, Beechhimeji, Honjimeshi, Shirotamogitake, Shimokuren, Shimosukeso, Potato, Peonies (Shakuyaku), Shajin (Sanjin), Jasmine (Matsurika), Janohige Cowpea, shukukonasumiso, shukushamitsu (sand seed, shredded sand), juzudama, shuro “fruit”, shran, ginger palm, ginger (ginger), ginger hydrangea (Tsuneyama), shobu (bamboo shoot, root), shozu “fruit” "Shirakashi" Seeds , Silane (Bikkyu), White-faced butterfly “Seeds”, White clover (Clover), White-leafed adzuki (Chick-bone grass), White-faced Squirrel (Nioiris), White-faced Iris “Flower”, White-faced Lupine, White-faced Minami (Southern real), Cinchona sylbula, Ginkgo biloba (Ruika, Rika, Shenhua), Cinnamon, Cinnamum Cassia (Cinnamon), Watermelon (Sairan), Honeysuckle (Gin Silver Flower, Shinobu), Sorrel (Shigami), Swimberry "Fruit", Star Apple, Stevia, Strawberry "Fruit ”, Suzu-Psycho (Xucho), Sugina (question), Surihiyu (horse tooth, horse tooth soap), Plum“ fruit ”, Sulgaran (Oran), Scots pine“ Ball cone ”, Japanese larch, Japanese kizuta, Japanese walnut , Hawthorn, cypress, ivy Popo, Horse Chestnut (Mallonier), Pear "fruit", Prunus japonica, Elder, Prunus (Juniper, Juniper), Achillea millefol (milfoil), Rose, Pterodactyl, Alaskan Or horse mint, horseradish, budgerigar, peony (Ishizone), gecko (Ishiburo, also known as Dendrobium, for example, cherry blossom, brilliant brilliant, giant wolfberry, horned peacock, hornbill, brilliant wolf, clover ), Mallow, shiro, celery, celery, senkyu (river cucumber), senshinren (spinning ream), sendan, sennin cactus, senna “fruit, leaf”, Sennin (Daisatsu), Senburi (Drug), Senboku, Soka (Grass), Elephant Chew, Sobain, Buckwheat "Seeds", Somer potato, Broad bean, Daiou (Dahi), Daikon radish, Soybean, Daidai (Orange peel, Persimmon), Takasago Ruri Higotai (Southeast Indigo Head), Takasaburo (
旱 lotus grass), Takatodai (Daikan), Takarabi (狗狗), dark sweet cherry “fruit”, hollyhock, hollyhock (thyme, perilla), tachidokoro (hikai), tachiyanagi, tamarind “seed”, onion, beetle ( Pepper), taranoki "fruit, leaves, root bark", tarayou (large leaf winter blue, one leaf tea, bitter tea, bitter tea), dahlia (tenjiku button), tarui, tanukirime, tanshichiwa, tanjin (dansang), dandelion ) Or white dandelion or mouko dandelion, danmaru, cherry “fruit”, chigaya “fruit, root, bud”, chixetsu carrot (bamboo ginseng), chicory, champa piedra (tufted licorice), tulip, choco no stay, datura ( Artichoke) (蕪 夷), Korean mugwort (艾 leaves), Choreimaitake (猪苓), Chorogi, camellia, tsukushikaneran, tsukuritake (mushrooms), camellia, camellia, camellia, clover (lacquered licorice), camellia (duck moth grass), Tsuruzuki (Red Azuki), Tsurutsuchiakebi, Tsurudokudami (What Noodles), Tsuruna (Apricot), Tsuruninjin (Yotsuba Ginseng), Tsurume, Tsuwabuki, Deiko, Teuchigurumi, Duberry "fruit", Tengusa, Tencha (salmon tea), Tendai Yaku (glaze) , Toga (winter lion), Toka Kazura, Capsicum (Banban), Toki (Today), Tokakinka (Marigold), Toukachika, Tounabena (river cut), corn or corn hair (Southern shark), Torizumimochi (woman) Sadako) , Toxa (wood bandits), Dokudami (10 medicines), Tokon (rooting), Toshishi or bean pods or pears, Tochu (Tsunaka "bark, leaf, root"), ash (tomato), tomato, tragacanth, triacanthus "seed", Eustoma, Tormentilla, Doronoki, Toro-aoi, Tonzu bean, Nigerian berry "fruit", Chinese yam or wild yam (mountain medicine), Nagii-kada (butcher bloom), Naginata Koju, Nazuna, rapeseed, jujube (sword bean), jujube (Otsuchi), Naniwaibara (Golden Cherry), Navena (Continued), Nameko, Peanut (Peanut), Nanten (Nan Tenmami), Namban Karamushi (Hama), Nio-Ismille, Nigaki (Nagi), Nigamugi (Sweet) , Nikuzuku, Kay or Nikkei or Ceylon Nikkei or Yab Nikkei (Cinnamon) or cinnamon (katsushi), buntingbird, leek (lion), elderberry (bone, "fruit, flower, stem, leaf"), garlic (large coral), nurde (penta), leek, nemnoki or neb or nebi Or Nemlinoki or Zigokubana (community), Noazami (Otsuka), Neubara (fruit), Nozenkazura (Ryokan), Nogateto, Yarrow, Nodake (Mae Hu), Novara, Nomomo, Pineapple "fruit", Hibiscus ), Hylindough, Hauchiwa, Pau Dorse, Hakama Labosi (bone crushing), Hakkayumatou, Hakusanchidori, Hakusen (white ring skin), Hakuruberry “fruit”, Japanese coconut (breeding), Hazel (coconut), Hashiridokoro (roth root) , Basil, lotus (lotus, lotus, lotus), parsley (holland jellies), hadakamugi Batata, Hachiku or Madake (bamboo shoots), patchouli, mint (light load, light load leaf), yellow peas, pearl barley (Yokuinin), Hanasuge (wisdom mother), banana, Hanaka mint, Hanabishi (Shinshiri, Shishitsuri), vanilla bins, papaya, Hakkogusa (鼠 麹, pahalobo, hub “whole plant, stem, leaf”, paprika, oysters, hamago or honey bee (vine), hamasuge (kotsuko), kingfish (spotted lion), hermanus (maikai flower), kingfish (Beach windbreak), hamamelis, palm, rose (rose), balata, balata rubber tree, agaric (agaric, white-faced agaric, edulis), halin, parisurus (palatus), parietalia, harukanran, balsamina (garlic), haruni Skin, white skin ), Barbasco, Harlindow, Handaikai (Taihokai), Pannoki, Hinogi (Giraffe), Hikageturinjin (Participant), Pecan nuts, Higanbana (Ishizuchi, Bujushahua), Hygurmadaria, Higotai, Hishi (Hishimi) , Pistachio, beet, human camellia, cypress, daisies, daisies, daisies, cypresses, cypresses, hiba, castor, sunflower, peppers, himeuzu, tencho, himematsutake (Kawariharatake, Hiromatsutake), Himemuyoran, Pimenta "fruit", beak, beakkyu, hiyu "fruit", chickpea, lentil, bellowed oyster, white-bellied camellia, loquat "fruit, leaf, stem", betel wax Leather, eggplant), Pu'er tea (putum tea), sweet quail (south) Rattan), Japanese cypress, Japanese cypress poppy (Japanese winter flowers, Japanese winter leaf), Japanese basil (Orchid), Japanese bean (Japanese peas), Japanese red cherry (Chrysanthemum laevis), Japanese grapes "fruit, pericarp, seeds, leaves", beech, Japanese cypress bamboo Cordyceps, Brazilian licorice, Brazilian carrot, Brazilian palm, Brazilian elephant palm, black currant "fruit", blackberry, brunnen, plum "fruit", full celaria, blueberry, prune, floral blanka, blond psyllium , Bundo (mung bean), hazelnut, loofah, hetsukaran, safflower (safflower), heneken, belladonna, berry "fruit", persair, pervianburg, pereschia grandifolia, bengiso or Ixusa (jingten), boysenberry "fruit", Ho Giant or sardine or nendo or hahagigi kokia (skin pupa), hosairan, spinach (acupuncture, acute pupa, teleost), pouteria sapota, pouteria rukuma, cypress, spinach, physalis (Torune), honoki (Japanese) Pak, Pak, Bokeh, Azalea, Butterweed, Butterweed, Prunus, Prunus, Ginseng, Kojiten Bark, Bodaju, Bamboo , Stalk, bark "), button pi (peony skin), hop, jojoba, borage (Lulichisha), Bordeaux, white berry" fruit ", hononiku (meat juju, daige), mizurutennansho (tennansei), maitake (Maiko), Maou (mao), Maca, Macadamia nuts, Margaret (Mokushungiku), Macri (Sea Grass), mugwa (mulberry white bark “bark, leaf”), magnolia sprengueri, masaran uba, masaran uba de sear, masheira de boi, mashua (tamanozenhalen, kyukonkinrenka), matatabi , Matsukasa, Pine “Leaf, Bark, Root”, Matta Orillo, Matsuhodo (Mushroom), Matico (Cordon Sillo), Mayoram (Hanahakka), Malvanojadine (Bitter), Mulberry “Fruit”, Quince, Malene (Billow Mouica) , Mango, Mangosteen, Manzanya (Andesca mitsuru), Manshugurumi, Mandarin “fruit”, Mannentake (reishi), Pheasant (fruit) “Mishimai psycho (Shibahu), Psyllium sorghum (Oldweed), Mizokakushi (Haren), Misohagi (Chikuma), Michiana Giant or elder willow (Hongshu), Mitsuwashi, Mitsuba, Midorisapote, Midorihakka, Mimosa, Myoga, Miracle fruit "fruit", Myrrh, Mybaran, Mugiwaragiku, Mukuge (Kiso), Mukunoki, Mukuroji (life extension skin), Munya, Murasaki ), Murasakikikibu or Omurasaxikibu (purple pearl), Murasaki corn, Murasaki Kinafuji (Kunming chicken blood wisteria), Megakansa opuntica, Swordfish (beneficial herb), Mebogi, Japanese evening primrose (Evening primrose), Melaleuca, Melissa, Melilot, Melon "fruit" , Moukomugi, Mosouchiku, Mokkirin, Moje (Pepper), Mochinoki, Mokko (woody incense), Japanese maple, Peach (peach “leaves, seeds, flowers, fruits”), Japanese coconut, Morello cherry “fruit”, Morohaya (burlap) ), Yakawa Murasaki Mo, Yakuchi (Michichi), cornflower (cornflower), cornflower mint, yacon, yaba santa, yashabushi (yash) or himeya shabushi or oyabaya sabushi "fruit, pericarp, fruit ear", yachiyanagi, scorpion (octagonal gold plate), mistletoe (willow parasitism) ), Willow (kawayanagi, chili willow, weeping willow, red willow, cat willow, Incolyana nagi, Kinu willow, Kolyanagi, Unryu willow, Miyayanagi, palm, Yamayanagi, Obayanagi, Tarikikunayana (Purple gold beef), Yabu tobacco (crane basket, Tenmeisei), pokeweed (commercial land), Yamahanoki (Yamamuro), yamamomo (Yamamushi), sagebrush, eucalyptus, yukinoshita (tiger ear grass), yucca or flevifolia, yuzu " Fruit, Lily, Wormweed, Mugwort (Leaf), Ligan (Raimaru), Lime “Fruit”, Rye, Lacanca “Fruit”, Raspberry “Leaf, Fruit”, Latania (Klamelia), Lackey or Shallot (Purple), Lavender , Longan (longan), agave (Aonu agave, fuchlin agave), ryokcha (green tea), apple "fruit, seeds, leaves, roots", gentian, lubas or suabisimus (cool), rulitama thisami (urajirohigotai) Head), litchi (toothpick, toothpick core), lettuce (chisha), red currant "fruit", red pitaya, lemon "fruit", lemongrass, forsythia or syringae (ream), forsythia, wintersweet, wax palm, Locon (Yoshi, Sone), Loganberry "fruit", Rose Marie (Mannenrou), Rosehip (Novara), Wasabi, Watafujitsugi (Mitsumonflower), Waremokkou (earth).

  ii) As seaweeds, for example, seaweed [Green algae: Chlorella vulgaris, Chlorella pyrenoidosa, Chlorella ellipsoidia, Aonori (Usubaaonori, Sugiaonori, Hiraaoori, Boaonori, Hosoeda Aonori), Anaaaosa (Aosaa)] Class: Kombu (Macombu, Resilibu, Hosome Kombu, Mitsuishi Kombu), Wakame, Hirome, Aowakame, Giant Kelp (Macrocystis pirifera, Macrocystis integrifolia, Neocites Luetkeana), Hijiki, Hibamata, Umiuchiwa Kirebano Miu Chiwa, Aka Ba Miu Chiwa, Kona Miu Chiwa, Okinawa Chiwa, Usuki Uchiwa, Etsuki Miu Chiwa], Seaweed [Red Algae: Red-tailed Men, Maxa (Tengusa), Hiluxa, Oni Sasa, Otoweed, Kata Otosa, Yatabegusa, Yuikiri, Shimatengusa, Tosakanori, Togekirinsai, Amakusa Rhinoceros, Giraffe Rhinoceros, Scotskin Giraffe, Tsunogata , Shikinori, kainori, yareus banori, kagius banori, sugius banori, haius banori, red fir tree] etc.

  iii) Examples of other algae include, for example, green algae (Chlamydomonas: Chlamydomonas, Akayukimo, Dunaliella: Dunaliella, Chlorococcus, Chlorococcus: Kuwanomimo, Volboxa: Ohigemawari, Volbox, Palmera, Yotsumemo, : Hizaori, Aoumikuro, Tsurugimidoro, Hibidoro: Hibidoro, Aosa: Anaaaosa, Amiaosa, Nagaaosa, Kawanori: Kawanori, Friccella, Shiogusa , Genus Magatama: Magamata, Iwata: Fusaiwata, Surikogita, Heraiwata, Kurokita, Hanemoga, Mille: Mill, Chromel, Sakibutimiru, Naga Lem, Hiramil, Casanori: Casanori, Jusmo: Futjusmo, Tamajusmo, Mizojusmo, Mizumakimo, Korekaete, Tsutsumimo, Kikumogusa: Kikkougusa, Hitogususa: Hitohagusa, Shirohaemu Genus, Sumiremo: Sumiremo, Hosimidoro, Fuchinashimidoro, etc., Cyanobacteria (Suizenjinori: Suizinoriori, Aoko, Nenjumo: Kawatake, Ishiage, Hasai, Yuremo, Rasenmo (Spirulina): Spirulina, Trichodesmium Iacacio), etc.), brown algae (Piraera genus: Piraela, Shiomidro genus: Nagamisiomidro, Iso grape genus: Iso grapes, Isogawara genus: Isogawara, Black moth genus: Gunsen kurogashira, Casilla Genus: Kashirazaki, Muchimo: Muchimo, Hiramchimo, Keberigusa, Ajigusa: Ajijigusa, Sakibiro-Amiji, Sanadagusa: Sanadagusa, Fukurimamiji, Commonusa Umichiwa, Kona Umichiwa, Akabami Uchiwa, Nami-makura: Hirami-makura, Soma-wagusa: Somerna, Navarimo, Nanaimo, Chasomen-genu Niseft mozuku, fumozuku genus: futo mozuku, ishimozuku genus: ishimozuku, kuromo genus: kuromo, nisemozuku genus: nisemozuku, mozuku genus: mozuku, ishige genus: i Shige, Iroro, Ichimegasa genus: Ichimegasa, Quercus genus: Quercus, Cyprinus genus: Umibosu, Urushigusa: Urushigusa, Keurushigusa, Tobacco mosquito, Crested genus: Crested genus, Haberodoki: Haberodoki, Habanorisei, Habari Common Bukuro: Common Bukuro, Ezobukuro: Ezobukuro, Fukuronori: Fukuronori, Cottontail, Chishima Fukuronori: Chishima Fukuronori, Kagomenori Genus: Murachidori: Murachidori, Shazugusa: Shazugusa, Iwahino Yori Genus: cynomolgus, genus genus: fennel, genus: genus, genus: turtle, genus: shark, genus: suji, misjikonbu: misjikonbu, azubamisujikon , Kombu genus: Gatsukira Kombu, Oyster Kombu, Oni Kombu, Gohei Kombu, Naga Kombu, Pea Kombu, Ouchiji Kombu, Trollo Kombu genus: Toro Kombu, Antokume genus: Antokume, Kajime genus: Kajime, Tsuruarame, Kurome, Kikushi Kombu, Genus Kombu Kuroshiome: Kuroshiome, Nekoashikonbu, Nekoashikonbu, Arame: Alame, Ainuwakame: Ainuwakame, Chigaiso, Oniwakae Ezoishige: Ezoi Shige, Yabanomoku: Yabanomoku, Rappamom: Johanomoku, Joromoku , Honda Walla: Tamanashimoku, Isomoku, Nagashimamoku, Akamoku, Shidamoku, Honda Walla, Nejimoku, Narasamo, Mametawala, Tatsukuri, Yatsumatamo , Umitorano, Obamoku, Fushishimoku, Hahamoku, Togemoku, Yoremok, Sawtooth Moku, Obanokogurimok, Sugimoku: Sugimoku, Ukimo: Ouomomo, Burumomo, Ukiboshi, Ushio Amanori: Asakusanori, Susabinori, Uppurinori, Oniamanori, Tasa, Firitasa, Benitasa, Rhodocolton: Milnobeni, Akebonomozuku: Akebonomozuku, Konahada, Yokorenohadada : Benimozuku, Hosobenimozu, Kasamatsu (Pinus genus): Kasamatsu, Fusanori: Fusanori, Nisefusanori: Nisehusanori, Sodegarami: Sodega Rami, Rattlera: Rattle, Rattlera, Hirohatamitaki: Hirohatamitaki, Tamaitaki: Tamaitaka, Kaikekenori: Kaginori, Kaikekenori, Proboscis, Hytengusa, Obuza, Hibusa, Hibusa , Cornflower, genus genus, genus genus, red moth, Rattlefish, Shiogusagoromo: Shiogusagoromo, Iwanokawa: Etsukiiwanoka, Kainokawa: Kainokawa, Kanino Genus: Canninote, Coral spider: Coral spider, Centipede sp. Genus: Matabou, Kintoki: Chabokintoki, Kintoki, Matsunori, Kori nori, Tosakamatsu, Hitomitsu, Fusikintoki, Tsunomukade, Nagakintoki, Sujikade, Kakreito: Oobakintoki, Atomugi, Aochoana, Amana , Mafonori, burrowing genus: burrowing crabs, cresting genus: crested burdock, broad-banded burdock, black-tailed burdock, black Sakamodoki, Nezushinotosakamodoki, Yusora, Etsunotosakamodoki, Nankaitosakamodoki, Himetosakamodoki, Kinu Hada: Kinu Hada, Ezotosaka genus: Ezotosaka, Tsukasano genus, Otsuka Nori, Hatsukiokaori : Chrysanthemum, Usuginu, Nikuhounoo: Nikuhounoo, Venusnago: Venusnago, Suscabeni: Suscabeni, Okaragusa: Porcupine, Mirrin: Mirin, Hosobamirin, Tosakanori: Isotari, Ezocari, Ezocari Yukari, Hosoyukari, Ibaranori: Ibaranori, Saidaibara, Tachiibara, Kagiibaranori, Kizinomo: Kizinoo, Isodantsu: Isodantsu, Atsubano Genus: Atsubanori, Ogonori: Ogonori, Tsurushiramo, Shiramo, Oogonori, Mizokonori, Birch, Beniogonori, Hukureshinori, Murasakibibanori, Shinkabanori, Togekabanori, Katagogonori, Ryukyugogori Ogonori, Mosagogonori, Kinubanori, Amanus genus: Hachijotensamodoki, Fushikurenori: Fushikurenori, Namiiwatake: Namiiwatake, Kamensou: Kamensou, Otsunori, Sakhari Genus: Ikanoashi, Hosobonori, Novonori, Black-backed Ginkgo: Black-backed Ginkgo, Red-backed Ginkgo Black genus: Hoshibukuro, Madaragusa: Togemadara, Etsumadara, Taoyagisou: Taoyagisou, Hanasakura, Fukutsunagi: Fukutsunagi, Sugikonori, Hananoeda: Hananoeda, Hiratakayagi, Darusugusa, Dasu Benifukuronori: Benifukuronori, Fusunagi: Fusutunagi, Himefushitsugi, Hirohafushitsugi, Otsunagiso: Hirawatsugiso, Usuwawatsunagiso, Igisu, Genus: Igis, Keigis, Harigis, Hanegis , Chrysanthemum sp. Nohanori nori: Nagakonohanori, Sugiginu: Sugiginu, Atsubasjiginu, Heiusbanori: Kaiusubanori, Yareusbanori, Sususbanori, Heiusbanori, Usbanomodia, Yayanishia, Yayanishikia, Yayanishi Isohagi, Shimadadia, Dajimodoki: Daijimodoki, Engussa: Moroitogusa, Fuyugusa, Makuri: Makri, Willow-nosed: Hanayanagi, Yuna, Yanagiri, Motsureyuna, Venetiancoly, Mosayanoso, Sahayazonoso, Hanazozo Genus: Hanegusa, Kenyagusa, Kozanemo Genus: Kozanemo, Isomurasaki, Hosozozanemo, Himegoke: Himegoke, Black Himego, Hyodosig Kuhodoshi, Hyodosigusa, Usubahiodo, Isomegusa: Isomegususa, Suginasushigusa: Susujinashigusa, Isobashogusu: Isobasho, Fujimatsumo: Fujimatsumo, Shirahiboshi: Hakusakumikohikawa, Hakusokumoku Ochimomok genus: Ochimomoku, Otosa, Chinorimo: Chinorimo, Chisujinori genus: Chisujinori, etc. Yellow algae (genus Hikarimo: Hikarimo etc.), etc.

  iv) As raw materials derived from animal raw materials, chicken crown extract, cattle / pig / fish / human placenta extract, beef / pig / fish stomach, duodenum, intestine, spleen extract or degradation product thereof, cattle / pig・ Fish brain tissue extracts, cattle / pigs / fish collagen hydrolysates (acids, alkalis, enzymes, etc.) degradation products, collagen derivatives such as water-soluble collagen or acylated collagen, cattle / pigs / fish elastin or elastin hydrolysates Degradation products (acid, alkali, enzyme, etc.) or water-soluble elastin derivatives, keratin and its degradation products or their derivatives, silk proteins and their degradation products or their derivatives, cattle / pig / fish blood cell protein degradation products (globin peptides) ), Hemoglobin degradation products (hemin, hematin, heme, protoheme, heme iron, etc.), cow milk, casein and its degradation products or their derivatives, skim milk powder and so Degradation products or derivatives thereof, lactoferrin or a degradation product thereof, egg ingredients, fish hydrolyzate, nucleic acid-related substance (ribonucleic acid, deoxyribonucleic acid), or the like.

v) Arbitrary sites, cells, tissues, organs, metabolites, etc. derived from genetically modified organisms or cell fusions of materials derived from plant or animal materials. Furthermore, for example, an undifferentiated cell group or a cell group in the middle of differentiation, etc. obtained by culturing cells of an arbitrary site, cell, tissue, organ or the like.
These various additives derived from plant or animal raw materials are processed in a conventional manner according to the product type and form to be added (for example, pulverization, milling, washing, hydrolysis, fermentation, purification, pressing, Extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. are arbitrarily selected and combined), and selected from various materials. It ’s fine.
The solvent used for extraction may be selected in consideration of the intended purpose and type of the product to be provided or the processing to be performed later. Usually, water, methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol , 1 type selected from various organic solvents such as lower alcohols such as isobutanol or hydrous lower alcohols, polyhydric alcohols such as propylene glycol, 1,3-butylene glycol and glycerin or hydrous polyhydric alcohols, acetone and ethyl acetate Alternatively, it is desirable to use a mixture of two or more. However, when it is not preferable to contain an organic solvent depending on the application, it is sufficient to use only water or ethanol that can be easily removed after extraction, and it may be used alone or in any mixture with water. The thing extracted and extracted may be sufficient.

  (14) Marine components; seawater such as deep seawater, for example, sea salt, dried seawater, dead sea or Atlantic or Pacific sea water (sodium chloride, magnesium chloride, potassium chloride, etc.), sea mud or Mud (Fango), for example, sea mud or mud from various locations such as Italian Fango, German Fango, Eifel Fango, Freiburg Fango (Contained components: silicon dioxide, titanium dioxide, aluminum oxide, iron oxide, manganese oxide, sodium oxide, oxidation Potassium, magnesium oxide, calcium oxide, strontium oxide, sodium, potassium, magnesium, calcium, chromium, iron, copper, nickel, zinc, lead, manganese, arsenic, water), saintoku stone, black meteorite, etc.

(15) Microbial culture metabolites; yeast metabolites, yeast extract, bacterial metabolites, bacterial extract, mold or actinomycete metabolites, mold or actinomyces extract, natto metabolites, natto extract, rice fermentation Extract, rice bran (red rice bran, white rice bran) fermented extract, Euglena extract or decomposition product thereof or water-soluble derivative thereof, trehalose or derivative thereof, lactic acid fermentation product of raw milk or skim milk powder, lactic acid bacteria fermentation product of legumes, coconut palm plant Lactic acid bacteria fermented products.
(16) α-hydroxy acids; glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.
(17) Inorganic pigments: silicic anhydride, magnesium silicate, talc, kaolin, bentonite, mica, mica titanium, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide , Bengala, black iron oxide, Gunjo, chromium oxide, chromium hydroxide, carbon black, calamine, etc.

  (18) UV absorber / blocker; benzophenone derivative (2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium, Dihydroxydimethoxybenzophenone, dihydroxydimethoxybenzophenone-sodium sulfonate, 2,4-dihydroxybenzophenone, tetrahydroxybenzophenone, etc.), paraaminobenzoic acid derivatives (paraaminobenzoic acid, ethyl paraaminobenzoate, glyceryl paraaminobenzoate, amyl paradimethylaminobenzoate) Octyl paradimethylaminobenzoate), methoxycinnamate derivatives (ethyl paramethoxycinnamate, isopropyl paramethoxycinnamate, paramethoxycinnamon) Octyl, 2-ethoxyethyl paramethoxycinnamate, sodium paramethoxycinnamate, potassium paramethoxycinnamate, diparamethoxycinnamate mono-2-ethylhexanoate glyceryl, etc.), salicylic acid derivatives (octyl salicylate, phenyl salicylate, homomenthyl salicylate, dipropylene glycol salicylate) , Ethylene glycol salicylate, myristyl salicylate, methyl salicylate, etc.), anthranilic acid derivatives (such as methyl anthranilate), urocanic acid derivatives (urocanic acid, ethyl urocanate etc.), coumarin derivatives, amino acid compounds, benzotriazole derivatives, tetrazole derivatives, Imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives, nucleic acid derivatives, alane In derivatives, nicotinic acid derivatives, vitamin B6 derivatives, umbelliferone, esculin, benzyl cinnamate, cinoxalate, oxybenzone, dioxybenzone, octabenzone, soribenzone, benzoresorcinol, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, Escalol, zinc oxide, talc, kaolin, etc.

  (19) Whitening agent; paraaminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound, benzotriazole derivative, tetrazole derivative, imidazoline derivative, pyrimidine derivative, dioxane derivative, camphor derivative, furan derivative, pyrone derivative, Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives, vitamin C or derivatives thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), vitamin E or derivatives thereof, kojic acid or derivatives thereof, oxybenzone, benzophenone, arbutin, guaiazulene, Shikonin, baicalin, baicalein, berberine, placenta extract, ellagic acid, lucinol, etc.

  (20) Tyrosinase activity inhibitor; vitamin C or a derivative thereof (vitamin C phosphate magnesium salt, vitamin C glucoside, etc.), hydroquinone or a derivative thereof (hydroquinone benzyl ether, etc.), kojic acid or a derivative thereof, vitamin E or a derivative thereof , N-acetyltyrosine or its derivatives, glutathione, hydrogen peroxide, zinc peroxide, placenta extract, ellagic acid, arbutin, lucinol, silk extract, plant extract (camille, mulberry, gardenia, toki, bittersweet, clara, mugwort, Honeysuckle, Yellowfin, Butterflies, Pinewood, Barley, Oyster Saw, Hops, Hawthorn, Eucalyptus, Achillea millefolium, Altea, Keihi, Mankeishi, Hamamelis, Karaguwa or Yamaguwa, Lifelong Grass, Kikyo, Toshishi, Jyogo, Ibuki, Mao Sinensis, Dokkatsu, Psycho, storm, littoralis, Scutellaria, peony bark, peony, cranesbill, kudzu root, licorice, Fushi aloe, Cimicifuga, safflower, green tea, black tea, Ali elixir), and the like.

(21) Melanin pigment reduction / decomposition substance; phenylmercury hexachlorophene, mercuric oxide, mercuric chloride, hydrogen peroxide, zinc peroxide, hydroquinone or its derivative (hydroquinone benzyl ether).
(22) Turnover promoting action / cell activator: hydroquinone, lactic acid bacteria extract, placenta extract, ganoderma extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract (aloe , Ogon, Horsetail, Gentiana, Burdock, Sicon, Carrot, Clam, Hop, Yokuinin, Odori, Semburi, Toki, Tokinsenka, Achacha, Hypericum, Cucumber, Tachikaku, Mannenrou, Parsley).

(23) Astringent: succinic acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc paraphenol sulfonate, potassium aluminum sulfate, resorcin, ferric chloride, tannic acid (including catechin compound) and the like.
(24) Active oxygen scavenger; SOD, catalase, glutathione peroxidase and the like.
(25) Antioxidant: Vitamin C or a salt thereof, stearic acid ester, vitamin E or a derivative thereof, nordihydrogua ceretenoic acid, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxy Anisole, propyl gallate, sesamol, sesamorin, gossypol, propolis, etc.

(26) Lipid peroxide production inhibitor: β-carotene, plant extract (sesame culture cell, hamcha, hypericum, clams, clove, melissa, birch, birch, salvia, mannenro, minamitenji, agetsu, ginkgo, green tea) and the like.
(27) Anti-inflammatory agent: ictamol, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d-camphor, dl-camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid or Its salt, glycyrrhetinic acid or its salt, licorice extract, coconut extract, age extract, propolis, etc.

  (28) Antibacterial / bactericidal / disinfectant: Acrinol, sulfur, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or hydrolyzate thereof, alkyldiaminoethylglycine chloride solution, triclosan, sodium hypochlorite, chloramine T , Salicy powder, iodine compound, iodoform, sorbic acid or salt thereof, propionic acid or salt thereof, salicylic acid, dehydroacetic acid, parahydroxybenzoic acid esters, undecylenic acid, thiamine lauryl sulfate, thiamine lauryl nitrate, phenol, cresol, p -Chlorophenol, p-chloro-m-xylenol, p-chloro-m-cresol, thymol, phenethyl alcohol, o-phenylphenol, Irgasan CH3565, halocarban, hexachloroph , Chlorohexidine, ethanol, methanol, isopropyl alcohol, benzyl alcohol, ethylene glycol, propylene glycol, 2-phenoxyethanol, 1,2-pentanediol, zinc pyridione, chlorobutanol, isopropylmethylphenol, nonionic surfactant (polyoxy Ethylene lauryl ether, polyoxyethylene nonyl phenyl ether, polyoxyethylene octyl phenyl ether, etc.) amphoteric surfactant, anionic surfactant (sodium lauryl sulfate, lauroyl sarcosine potassium, etc.), cationic surfactant (cetyltrimethylammonium bromide) Benzalkonium chloride, benzethonium chloride, methyl rosaniline chloride), formaldehyde, hexamine, brilliant green, ma Kite Green, Crystal Violet, Jamaru photosensitive No. 101-containing, photosensitizer No. 201, photosensitizer No. 401, N- long chain acyl basic amino acid derivative and its acid shark salts, zinc oxide, hinokitiol, Sophora root, propolis, and the like.

  (29) Moisturizer; glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, glycerin tricaprycapric acid, glycolic acid (α-hydroxy acid), hyaluronic acid or its salt, chondroitin sulfate or its salt, water-soluble chitin Or derivatives thereof or chitosan derivatives, pyrrolidone carboxylic acid or salts thereof, sodium lactate, urea, sorbitol, amino acids or derivatives thereof (valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, Serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine, Sulfates, phosphates, nitrates, citrates, or pyrrolidone carboxylic acids), fats and oils “Avocado oil, almond oil, fennel oil, sesame oil, olive oil, orange oil, orange rafa oil, sesame oil, cocoa butter, Chamomile oil, carrot oil, cucumber oil, beef tallow fatty acid, quince seed oil, cucumber nut oil, safflower oil, shea fat, liquid shea fat, soybean oil, camellia oil, corn oil, rapeseed oil, jojoba oil, persic oil, castor oil , Sunflower oil, cottonseed oil, peanut oil, turtle oil, tea tree oil, mink oil, egg yolk oil, palm oil, palm kernel oil, molasses, coconut oil, eucalyptus oil, beef tallow, pork fat, squalene, squalane, pristane or these oils and fats Hydrogenated products (hardened oil) ", waxes (bee wax, carnauba wax, whale wax, lanolin, liquid lano Phosphorus, reduced lanolin, hard lanolin, candelilla wax, montan wax, shellac wax, rice wax), mineral oil (liquid paraffin, petrolatum, paraffin, ozokelide, ceresin, microcristan wax), natto bacteria metabolites, natto extract, silk fiber extract , Plant extracts (red pine sap, aloe, kudzu, chamomile, licorice, cucumber, licorice, rice or rice bran, purple root, birch or white birch sap, assembly, mulberry white skin, birch leaf, loofah, peony skin, persimmon) and the like.

(30) Elastase activity inhibitor; diisopropyl fluorophosphate, plant extract (Ogon, Hypericum, Clara, Mulberry leaf, Keihi, Genso pepper, Comfrey, Salvia, Elderberry, Bodaige, Buttonpi), seaweed extract and the like.
(31) Hair preparation; selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castari tincture, pepper tincture, pepper tincture, quinine hydrochloride, strong ammonia water, potassium bromate, sodium bromate, Thioglycolic acid and the like.
(32) Antiandrogens; follicular hormones (estrone, estradiol, ethinyl estradiol, etc.), isoflavones, oxendrone, etc.

(33) Peripheral vascular blood flow promoters: vitamin E or derivatives thereof, assembly extract, garlic extract, carrot extract, aloe extract, gentian extract, toki extract, cephalanthin, carpronium chloride, minoxidil and the like.
(34) Stimulants: chili pepper tincture, nonyl acid vanillamide, cantalis tincture, ginger tincture, peppermint oil, l-menthol, camphor, benzyl nicotinate and the like.
(35) Metabolic active agent: Photosensitive element 301, hinokitiol, pantothenic acid or derivatives thereof, allantoin, placenta extract, biotin, pentadecanoic acid glyceride and the like.
(36) Antiseborrheic agent; pyridoxine or a derivative thereof, sulfur, vitamin B6 and the like.

(37) Keratolytic agent; resorcin, salicylic acid, lactic acid, urea and the like.
(38) Oxidizing agent; hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, sodium pyrophosphate Sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate hydrogen peroxide adduct, sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract, etc.
(39) Hair remover; inorganic reducing agent such as strontium sulfate, sodium sulfide, barium sulfide, calcium sulfide, thioglycolic acid or salts thereof (calcium thioglycolate, sodium thioglycolate, lithium thioglycolate, thioglycolic acid) Magnesium, strontium thioglycolate) and the like.

(40) Hair swelling agent; ethanolamine, urea, guanidine and the like.
(41) Dye agent; 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-amino-4-methylaminoanthraquinone, 3,3′-iminodiphenol, hydrochloric acid 2,4-diaminophenoxyethanol, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, metaphenylenediamine hydrochloride, orthoamino Phenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene-3,4-diamine, nitroparaphenylene Diamine, para-ami Phenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, picramic acid, sodium picramate, N, N′-bis (4-aminophenyl) -2,5-diamino-1,4-quinonediimine, 5 -(2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaaminophenol, metaphenylenediamine, 5-aminoorcresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate , Orthochloroparaphenylenediamine sulfate, 4,4′-diaminodiphenylamine sulfate, 2,4-diaminophenol sulfate, toluene-2,5-diamine sulfate, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, parasulfate sulfate Nitroorthophenylenediamine, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol, diphenylamine, α-naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, Resorcin, tannic acid, 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzene-5'-sodium sulfonate, hematein and the like.

  (42) Fragrances: Natural animal fragrances such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, guayakwood essential oil , Cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, gingergrass essential oil, cedar essential oil, spearmint essential oil, western peppermint essential oil , Otsuka perfume essential oil, tuberose essential oil, clove essential oil, orange flower essential oil, winter green essential oil, trout balsam essential oil, buttery essential oil, rose essential oil, palmarosa essential oil, persimmon essential oil, hiba essential oil, sandalwood essential oil, petitgren essential oil, bay essential oil, vetiva essential oil, Bergamo Essential oil, peruvian balsam essential oil, boad rose essential oil, melamine essential oil, mandarin essential oil, eucalyptus essential oil, lime essential oil, lavender essential oil, linaloe essential oil, lemongrass essential oil, lemon essential oil, rosemary (mannenrou) essential oil, Japanese mint mint essential oil, etc. Sexual fragrance, other synthetic fragrances, etc.

(43) Dye / colorant: red cabbage dye, red rice dye, red dye, anato dye, squid dye, turmeric dye, enju dye, krill dye, carp dye, caramel, gold, silver, gardenia dye, corn dye, onion Pigment, tamarind dye, spirulina dye, buckwheat whole plant dye, cherry dye, laver dye, hibiscus dye, grape juice dye, marigold dye, purple potato dye, purple yam dye, lac dye, rutin etc.
(44) Others: Hormones, sequestering agents, pH adjusters, chelating agents, antiseptic / antibacterial agents, cooling agents, stabilizers, emulsifiers, animal / plant proteins and their degradation products, animals / plants Saccharides and their degradation products, animal and plant glycoproteins and their degradation products, blood flow promoters, anti-inflammatory agents, anti-inflammatory agents, anti-allergic agents, wound treatment agents, foam enhancers, thickeners, enzymes, purified water ( Electronic water, small clusters, etc.), oral preparations, deodorants / deodorants, bitters, seasonings, etc.

In addition, when the additive derived from the plant or animal material of (13) and the deep sea water of (14) are used for systemic or topical external preparations and cosmetics, the protection of skin and hair is started. , Moisturizing, improving touch and texture, imparting flexibility, reducing irritation, reducing stress due to aroma, cell activation (preventing cell aging), suppressing inflammation, improving skin and hair quality, preventing rough skin and improving it, In addition to cosmetic effects such as hair growth, hair growth, hair loss prevention, gloss imparting, cleansing effect, fatigue relief, blood flow promotion, warm bath effect, etc., anticipation, deodorization, thickening, antiseptic, buffering, etc. are also expected it can.
The cosmetic composition of the present invention is used as an intermediate for various cosmetics and quasi-drugs, or as a product as it is. Specific examples of various cosmetics and quasi-drugs include basic cosmetics such as lotions, emulsions, creams, ointments, lotions, oils, packs, facial cleansers, skin cleansers, massage agents, and cleansings. Topical, whole body, such as preparations, hair removers, hair removers, shaving treatments, aftershave lotions, pre-show lotions, shaving creams, foundations, lipsticks, blushers, eye shadows, eyeliners, mascaras, perfumes, etc. Cosmetics for skin, and shampoos, rinses, treatments, permanent liquids, hair dyes, hair styling agents, hair styling agents, medicinal and / or cosmetic preparations applied to the scalp and hair, such as hair growth and hair nourishing agents; Etc.

The cosmetic composition according to the present invention is obtained by blending a lignite extract extracted from lignite, and can effectively exhibit various functions of the lignite extract. Specifically, the lignite extract is excellent in a whitening action, an antioxidant action, an anti-aging action and the like.
Moreover, although lignite is a natural product, it is in a stable state due to long-term denaturation in the ground, so that there is no great variation in quality performance for each collected raw material such as animals and plants.
As a result, a cosmetic composition having stable quality and performance can be produced industrially and efficiently, and the performance of the cosmetic composition can be improved, which can greatly contribute to the improvement of productivity.

The specific example of a cosmetic composition and the result of having evaluated the performance are shown.
[Manufacture of lignite extract and hot spring water extract]
<Example 1>
As lignite raw materials, those collected in Obihiro, Hokkaido were used.
The lignite raw material was pulverized to an average particle size of 5 mm. By adding 100 ml of water to 10.21 g of the pulverized product and subjecting it to an autoclave at 121 ° C. and 0.1 MPa for 20 minutes, an active ingredient was extracted from the lignite. The extract was cooled and filtered through a 0.45 μm filter to obtain a lignite extract.
The pH of the lignite extract solution was 4.4 and contained 0.01% solids.

<Comparative Example 1>
Mall hot spring water that came from the source of Tokachi hot spring in Hokkaido was used. In the same manner as in Example 1, it was subjected to an autoclave at 121 ° C. and 0.1 MPa for 20 minutes, cooled, and then filtered through a 0.45 μm filter to obtain a hot spring water extract solution.
The hot spring water extract solution had a pH of 8.8 and a solid content of 0.03%.
[Performance evaluation test]
Basically, measuring devices, measuring methods and procedures generally known in the technical field were adopted. Even if it is different from the test method described below, it is possible to adopt any test technique that can perform the same evaluation.

<Tyrosinase inhibitory activity>
To evaluate the inhibitory function on the activity of tyrosinase involved in the melanin synthesis process.
Take 1 ml of the measurement sample in a test tube, add 1 ml of 3 mM L-DOPA (0.2 M phosphate buffer: dissolved in pH 6.5) and 0.5 ml of 0.2 M phosphate buffer, and stir, then at 37 ° C. for 20 minutes. Warm up.
Separately, tyrosinase (derived from mushrooms) is mixed at 300 units / ml with 0.2 M phosphate buffer and heated at 37 ° C. for 20 minutes. After adding 0.5 ml of this tyrosinase solution to the measurement sample solution and stirring, the absorbance value is immediately measured using kinetic software. The measurement conditions are a measurement wavelength of 475 nm and a calculation interval of 0 to 15 seconds. As a control, 0.2 M phosphate buffer is added instead of the measurement sample, and the same measurement is performed.

The tyrosinase inhibitory activity rate is calculated by the following formula.
Tyrosinase inhibitory activity rate (%) =
[1-initial speed of measurement sample (OD / min) / initial speed of control (OD / min)] × 100
From the results, the concentration [IC50 (mg / ml)] required to inhibit the activity of tyrosinase by 50% is calculated in terms of solid content of the measurement sample.
<Melanin synthesis inhibitory activity>
B16 melanoma cells derived from a mouse that produces melanin were seeded in 6 ml at a concentration of 1 × 10 ⁵ cells / ml in a 6-well plate and cultured for 1 day under conditions of 37 ° C. and 5% CO ₂ . After removing the medium, a medium containing a measurement sample sterilized by filtration with a 0.2 μm filter at a final concentration of 6, 24, and 50 μg / ml is added, followed by culturing at 37 ° C. under 5% CO ₂ for 3 days. After removing the medium, the cells are detached with an EGTA-trypsin solution (PBS), the cells are collected in a 1.5 ml micro test tube, and centrifuged at 12000 rpm for 2 to 5 minutes. The supernatant is removed, 1 ml of 1N sodium hydroxide is added, and heat treatment is performed at 100 ° C. for 2 to 3 hours to extract the melanin pigment. The melanin pigment is measured with a spectrophotometer (405 nm). As a control, the same culture is performed using only the medium containing no measurement sample. The protein concentration of the cells is measured by [DC-Protein Assay (manufactured by BIO-RAD)].

The melanin synthesis inhibition rate is calculated from the melanin amount per total protein amount by the following formula.
Melanin synthesis inhibition rate (%) =
[1-melanin amount (measurement sample) / melanin amount (control)] × 100
From this melanin synthesis inhibition rate, the concentration [IC50 (mg / ml)] required to inhibit the synthesis of melanin by 50% in terms of solid content of the measurement sample is calculated.
<SOD-like activity>
Measurement is performed in a 96-well plate using [SOD Assay Kit-WST] manufactured by Dojindo Laboratories. Use the reagents that come with the kit. After adding 20 μl of sample solution to each well, add 200 μl of [WST working solution] and stir well with a plate mixer. Add 20 μl of [Dilution buffer] to each blank well. Add 20 μl of [Enzyme working solution] to each well containing the sample solution and blank wells. Thereafter, it is incubated at 37 ° C. for 20 minutes, and the absorbance value at 450 nm is measured with a plate reader.

The inhibition rate of superoxide is calculated by the following formula.
Superoxide inhibition rate (%)
= [{(E [BL] −E [BL-BL]) − (E [S] −E [S-BL])}
/ (E [BL] −E [BL-BL])] × 100
Each symbol in the above formula is an absorbance value measured under the following measurement conditions.

Based on the superoxide inhibition rate (%) of the measurement sample and the calibration curve based on the superoxide inhibition rate (%) of the SOD (unit) determined in advance, the activity function equivalent to that of the measurement sample is calculated in terms of solid content of the measurement sample. The amount of SOD units shown, ie the SOD-like activity value [SODunits / g] is calculated.
<OH scavenging activity>
Add 0.15 ml of the measurement sample to 0.5 ml of 150 mM NaCl and 0.2 ml of 5 mM deoxyribose, and stir. To this, 2 mM ammonium iron sulfate (II) is added, followed by 0.05 ml of 0.03% H ₂ O ₂ . Further, 0.5 ml of 1% thiobarbituric acid is added and 0.5 ml of 2.8% trichloroacetic acid is added and stirred. Then, after heating in a water bath at 100 ° C. for 15 minutes, immediately after cooling with ice, measurement is performed with a spectrophotometer (535 nm). As a control, distilled water is added instead of the measurement sample, and the same measurement is performed.

Calculate the OH erasure rate (%) by the following formula.
・ OH elimination rate (%) =
[1-OD535 nm (measurement sample) / OD535 nm (control)] × 100
From this OH elimination rate, the concentration [IC50 (mg / ml)] required to inhibit the activity of OH by 50% is calculated in terms of solid content of the measurement sample.
<Hyaluronidase inhibitory activity>
100 μl of an enzyme solution in which hyaluronidase is dissolved at 2000 NFunit / ml in 0.1 M acetate buffer (pH 4.0) is added to 200 μl of a measurement sample, and heated at 37 ° C. for 20 minutes. Thereafter, 200 μl of a solution in which compound 48/80 is dissolved at 0.5 mg / ml is added to 0.1 M acetate buffer (pH 4.0), and the mixture is heated at 37 ° C. for 20 minutes. Furthermore, 500 μl of a substrate solution in which potassium hyaluronate is dissolved at 0.4 mg / ml is added to 0.1 M acetate buffer (pH 4.0) and heated at 37 ° C. for 40 minutes. Thereafter, 200 μl of 0.4N NaOH is added, and after ice cooling, 200 μl of boric acid solution (5%, pH 9.1) is added and heated at 100 ° C. for 3 minutes. After ice cooling, 6 ml of 1% p-dimethylbenzaldehyde solution is added and heated at 37 ° C. for 20 minutes. Thereafter, the absorbance is measured with a spectrophotometer (585 nm).

The hyaluronidase inhibition rate (%) is calculated using the following formula. In the following formula, a buffer solution is a buffer solution obtained when the above operation is performed without adding a measurement sample, and a measurement sample is a solution obtained by performing all the above operations. . Moreover, a blank is what has not added the substrate solution.
Hyaluronidase inhibition rate (%) =
[(Value in buffer-blank in buffer)-(value in measurement sample-blank in measurement sample)] ÷ (value in buffer-blank in buffer) x 100
From this hyaluronidase inhibition rate, the concentration [IC50 (mg / ml)] required to inhibit hyaluronidase activity by 50% is calculated in terms of solid content of the measurement sample.

<Collagenase inhibitory activity>
50 μl of a measurement sample and 50 μl of collagenase solution (1 unit / ml) are added to 50 μl of a solution in which collagen labeled with FITC (fluorescein isothiocyanate) is mixed with 1 mg / ml of 5 mM acetic acid. This is warmed at 37 ° C. for 1 hour. Thereafter, the reaction was stopped by boiling, and 200 μl of a reaction stop extract composed of 60% EtOH, 0.5 M NaCl, 0.125 M Tris-HCl, and 80 mM o-phenanthroline was added, and then centrifuged by a centrifuge. Separate and collect the supernatant of the centrifuge, and measure the fluorescence (Ex: 495 nm, Em: 520 nm) of the recovered solution. At the same time, a calibration curve with different collagenase concentrations is prepared, and the concentration [IC50 (mg / ml)] required to inhibit collagenase activity by 50% is calculated in terms of solid content of the measurement sample.

<Test results>
The results of each test are shown in the table below.

<Evaluation>
Regarding evaluation tests for tyrosinase inhibitory activity, melanin synthesis inhibitory activity, OH scavenging activity, hyaluronidase inhibitory activity, collagenase inhibitory activity, the smaller the value of IC50 (mg / ml), the more the evaluation test for SOD-like activity As described above, the larger the value of SODunits / g, the better the cosmetic composition.
Therefore, in Table 2, when a lignite extract and a hot spring water extract are each mix | blended with cosmetics composition, it turns out that the performance of both becomes a thing with the better lignite extract in any performance.

[Cosmetics verification test]
Using the lignite extract solution and the hot spring water extract solution shown in Example 1 and Comparative Example 1 above, a specific extract-containing cosmetic was manufactured and its performance was verified.
<Manufacture of cosmetics>
In order to perform a sensory test of cosmetics, cosmetics were produced with the formulations shown in Table 3.
The lignite extract solution obtained in Example 1 was used, and the hot spring water extract solution obtained in Comparative Example 1 was used. As an additive-free zone, a mixture containing purified water instead of the lignite extract solution and hot spring water extract solution was also produced.

<Sensory test of cosmetics>
The manufactured cosmetics were subjected to a sensory test with a monitor (10 women each in their 20s to 50s) to evaluate the whitening effect, moisturizing effect, and anti-aging effect. As an index of the anti-aging effect, the improvement of wrinkles, tension and tarmi was evaluated. The monitor was applied with an appropriate amount of cosmetic liquid on the face after face washing twice a day in the morning and at night for two months, and judged according to the following criteria.
Effective: The improvement effect was recognized compared with before use.
Slightly effective: Slight improvement effect was observed compared to before use.
Invalid: No change compared to before use.

  The test results are shown in Table 4.

<Evaluation>
Referring to Table 4, the cosmetic composition containing the lignite extract liquid of Example 1 was more wrinkled, crumpled, and talmi than the hot spring water extract liquid or other cosmetic compositions containing water. It can be seen that the whitening performance is excellent.

The cosmetic composition of the present invention can be used for skin cosmetics, for example. By including the lignite extract, it is possible to exhibit excellent performances such as a whitening effect, an antioxidant effect, and an anti-aging effect. Since the lignite extract can be economically supplied with industrially stable quality performance, it is possible to economically supply high-quality and high-functional cosmetics.

Claims (3)

  A cosmetic composition comprising a lignite extract.   The lignite extract is extracted from lignite at an extraction temperature of 60 to 374 ° C., an extraction time of 1 second to 24 hours, and an extraction pressure of 0.1 to 30 MPa using water and / or an organic solvent as an extraction medium. The cosmetic composition according to claim 1. The cosmetic composition according to claim 1 or 2, wherein the lignite extract is blended in an amount of 0.001 to 100% by weight of the total amount.