JP2007282863A - Biosensor chip - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a biosensor chip which can be exchanged easily without taking off a protection cap on the distal end of a measurement device. <P>SOLUTION: Projections 13 arranged on a chip body 11 project from slits 33a of the protection cap 33 attached to the distal end of the measurement device 31. With such a configuration, the biosensor chip 10 is attached to or detached from the measurement device 31 easily without taking off the protection cap 33 of the measurement device 31. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a biosensor chip, for example, a biosensor chip that measures and analyzes a chemical substance using a reagent contained in a hollow reaction part of the chip.

Conventionally, a biosensor in which a biosensor chip and a lancet are integrated has been disclosed (see, for example, Patent Document 1).
5A is a perspective view of the sensor described in Patent Document 1, and FIG. 5B is an exploded perspective view of the sensor. As shown in FIG. 5, the lancet-integrated sensor 100 includes a chip body 101, a lancet 103, and a protective cover 105. The chip body 101 has a cover 101a and a substrate 101b that can be opened and closed, and an internal space 102 is formed on the inner surface of the cover 101a. The internal space 102 has a shape that can accommodate the lancet 103 in a movable manner.

  The needle 104 provided at the tip of the lancet 103 can be moved in and out from an opening 102 a formed at the front end of the internal space 102 of the chip body 101 as the lancet 103 moves. The shape of the internal space 101a is curved so that the width thereof is slightly narrower than the lancet 103 at the end where the protrusion 103a is located, and the lancet 103 is locked to the chip body 101 by the mutual pressing force and frictional force. It is like that. The protective cover 105 has a tube part 105 a into which the needle 104 is inserted, and the tube part 105 a can be accommodated inside the chip body 101 as the needle 104 moves. Therefore, in a state before use, the protective cover 105 is put on the needle 104 to protect the needle 104 and prevent the user from being accidentally injured. Note that the substrate 101b is provided with a pair of electrode terminals 106 so that it can be electrically connected to a measuring apparatus (not shown).

In use, the protective cover 105 is removed and the lancet 103 is pushed to cause the needle 104 to protrude from the chip body 101. After puncturing the subject in this state, the needle 104 is housed inside the chip body 101, and the opening 102a provided at the front end of the chip body 101 is brought close to the puncture port to collect the outflowed blood.
WO02-056769

  However, the lancet-integrated sensor 100 described in Patent Document 1 has a structure in which blood flowing out from the puncture port is absorbed from the opening 102a, but the structure is complicated.

  The present invention has been made in view of the above-described problems, and an object thereof is to provide a biosensor chip that can be easily replaced without removing the protective cap at the tip of the measuring device.

  In order to achieve the above-described object, a biosensor chip that is a first feature according to the present invention is a biosensor chip that collects a sample by driving a puncture device in the measurement apparatus. A protrusion that protrudes outward from the slit of the protective cap attached to the tip and is movable is provided.

  In the biosensor chip configured as described above, since the protrusion provided on the chip body protrudes from the slit of the protective cap provided at the tip of the measuring device, the user must hold the protrusion with a finger. Thus, the biosensor chip can be easily attached to and detached from the measuring device without removing the protective cap of the measuring device.

  A biosensor chip according to the second feature of the present invention is the biosensor chip according to the first feature of the present invention, wherein the protrusion is provided integrally with the chip body.

  In the biosensor chip configured as described above, when the chip body of the biosensor chip is resin-molded, the protrusion can be easily provided by simultaneously molding the protrusion integrally.

  Moreover, the biosensor chip according to the third feature of the present invention is the one having the elastic body covering the periphery of the puncture device at the tip of the chip body in the first or second feature of the present invention. It is.

  In the biosensor chip configured as described above, since the elastic body is provided at the tip of the chip body, the sample can be sucked after puncturing through the space of the elastic body, and the amount of the sample is small. However, the sample suction can be performed smoothly. Moreover, while protecting the puncture device which protrudes from the front-end | tip part of a chip | tip main body, a user can be protected. In addition, when the biosensor chip is driven by the driving means of the measuring device, the needle is extracted from the subject by the restoring force of the elastic body, and the sample flowing out from the puncture port is reliably collected via the elastic body. Can do. In addition, since the needle is covered so as not to protrude from the distal end surface of the elastic body when it is discarded after use, it can be disposed of safely and appropriately.

  The biosensor chip according to the fourth feature of the present invention is the biosensor chip according to the third feature of the present invention, wherein the tip of the chip body has a shape of a contact surface with the tip of the tip of the elastic body. It corresponds to.

  In the biosensor chip configured as described above, the shape of the contact surface with the tip of the tip of the elastic body provided at the tip of the chip body corresponds to the shape of the tip of the chip body. The elastic body can be securely attached, and can be prevented from coming off or slipping when a pressing force acts on the elastic body.

  According to a fifth aspect of the present invention, there is provided a biosensor system comprising: the biosensor chip according to any one of the first to fourth aspects of the present invention; and a measuring device for attaching the biosensor chip interchangeably. The measuring device has a slit that is provided at the tip of the measuring device so that the protrusion of the biosensor chip can move, and that protects the puncture device, and the sample body is driven by driving the chip body. And a driving means for puncturing.

  In the biosensor system configured as described above, the biosensor chip can be easily attached and detached by grasping the protrusion of the biosensor chip without removing the protective cap provided at the tip of the measuring device. In addition, the biosensor chip described above is attached to the driving means, the biosensor chip is driven by the driving means to puncture, the sample is collected, and the information on the sample is transmitted to the measuring device via the detection electrode. In addition, since the measurement can be easily performed, the burden on the subject can be reduced.

  According to the present invention, since the protrusion provided on the chip body protrudes from the slit of the protective cap provided at the tip of the measuring device, it is possible to remove the protective cap of the measuring device by gripping this protrusion. The biosensor chip can be easily attached to and detached from the measuring device.

DESCRIPTION OF EMBODIMENTS Hereinafter, embodiments according to the present invention will be described in detail with reference to the drawings.
FIG. 1 (A) is a front view showing a state in which the biosensor chip according to the first embodiment of the present invention is attached to a measuring device, FIG. 1 (B) is a side view seen from the B direction in FIG. 1 (A), 2A is a cross-sectional view of the biosensor chip, FIG. 2B is an end view of the biosensor chip viewed from the B direction in FIG. 2A, and FIG. 3 is a configuration diagram of the biosensor system.

  As shown in FIGS. 1 and 3, the biosensor chip 10 according to the first embodiment of the present invention is attached to and driven by the driving means 32 (see FIG. 3) of the measuring device 31, so that the chip body 11. A sample is punctured with a puncture needle 12 that is a puncture device provided to protrude from the distal end portion 11a, and a sample, for example, blood is collected. The chip body 11 is provided with a protrusion 13 that protrudes outward from the slit 33a of the protective cap 33 attached to the tip of the measuring device 31 and is movable.

  The puncture device of the present invention is a generic term for a needle, a lancet needle, a cannula and the like. The puncture needle 12 is preferably biodegradable.

  2A and 2B show an example of the biosensor chip 10. In the biosensor chip 10, the chip body 11 has two substrates 14a and 14b facing each other and a spacer layer 15 sandwiched between the two substrates 14a and 14b. Detection electrodes 16a and 16b are provided on the surface of at least one substrate 14a of the two substrates 14a and 14b on the spacer layer 15 side, and tip portions (lower end portions in FIG. 2A) are mutually connected. It is bent in an L shape in the opposite direction to maintain a predetermined interval. A hollow reaction portion 17 is formed by the two substrates 14 a and 14 b and the spacer layer 15 from the tip portion 11 a of the chip body 11 to a portion where the two detection electrodes 16 a and 16 b are opposed to each other. A sample collection port 17 a for introducing blood as a sample collected by puncturing the sample with the puncture needle 12 to the hollow reaction unit 17 is provided at the tip of the hollow reaction unit 17. In the hollow reaction part 17, the detection electrodes 16a and 16b are exposed, and, for example, an enzyme and a mediator are immobilized and reacted with glucose in blood immediately above or in the vicinity of the detection electrodes 16a and 16b in the hollow reaction part 17. A reagent 18 for generating an electric current is provided. Accordingly, the hollow reaction part 17 is a part where blood such as blood collected from the sample collection port 17a undergoes a biochemical reaction with the reagent 18.

  As the material of the substrates 14a and 14b and the spacer layer 15, an insulating material film is selected. As the insulating material, ceramics, glass, paper, biodegradable material (for example, polylactic acid microorganism-producing polyester), poly, etc. Examples thereof include thermoplastic materials such as vinyl chloride, polypropylene, polystyrene, polycarbonate, acrylic resin, polybutylene terephthalate and polyethylene terephthalate (PET), thermosetting resins such as epoxy resins, and plastic materials such as UV curable resins. A plastic material such as polyethylene terephthalate is preferable because of its mechanical strength, flexibility, and ease of chip fabrication and processing. Representative PET resins include Melinex and Tetron (trade names, manufactured by Teijin DuPont Films, Inc.), Lumirror (trade names, manufactured by Toray Industries, Inc.), and the like.

    Examples of the reagent 18 include glucose oxidase (GOD).

  In consideration of the blood sampling burden of the specimen, the volume of the hollow reaction part 17 is preferably 1 μL (microliter) or less, and particularly preferably 300 nL (nanoliter) or less. With such a small hollow reaction part 17, a sufficient blood volume of the specimen can be collected even if the diameter of the puncture needle 12 is small. Preferably, the diameter is 1000 μm or less.

Further, at least one of the substrates 14a and 14b (for example, the substrate 14a) forming the chip body 11 is provided with a protrusion 13 protruding in the width direction. The protrusion 13 is desirably provided integrally with the substrate 14a. That is, it is preferable to provide the protrusions 13 integrally when the substrate 14a is molded from resin. The protrusion 13 is larger than the outer shape of a protective cap 33 attached to the tip of a measuring device 31 described later, and has a size that protrudes outward from the slit 33a. In addition, it is desirable to provide the recessed part 13b in the front end surface 13a of the processus | protrusion 13, and to prevent it, when a user hold | grips with a finger, slips.
When the substrate 14a of the chip body 11 is resin-molded, the protrusion 13 can be easily provided by simultaneously forming the protrusion 13 integrally. The protrusion 13 can be provided on the substrate 14b. Or it is also possible to provide in both board | substrates 14a and 14b.

  As described above, according to the biosensor chip 10 described above, since the protrusion 13 provided on the chip body 11 protrudes from the slit 33a of the protective cap 33 provided at the tip of the measuring device 31, the user can use the protrusion 13 as described above. The biosensor chip 10 can be easily attached to and detached from the measuring device 31 without removing the protective cap 33 of the measuring device 31 by gripping the tip 13a (recessed portion 13b) with a finger. Thereby, a user's burden can be reduced.

Next, the biosensor system according to the present invention will be described. FIG. 3 shows a configuration of a biosensor system 30 using the biosensor chip 10 described above.
As shown in FIG. 3, the biosensor system 30 includes the biosensor chip 10 described above and a measuring device 31 that obtains information on blood collected by connecting to the detection electrodes 16 a and 16 b of the biosensor chip 10. Have. The configuration of the biosensor chip 10 is as described above, and the same reference numerals are given to the same parts as the biosensor chip 10 described above, and the description thereof is omitted here.

The measuring device 31 includes a power supply 34, a control device 35, and a display unit 36, which are connected to each other. The rear end portion 11b of the chip body 11 is inserted and fixed, and a terminal insertion portion 37 for electrically connecting the detection electrodes 16a and 16b to the control device 35 and the like is provided at the front end of the driving means 32. In a state in which the biosensor chip 10 is attached, the biosensor chip 10 is driven forward (leftward in FIG. 3) by, for example, a spring 38. The protective cap 33 provided at the distal end of the measuring device 31 has an internal space (not shown) in which the biosensor chip 10 can move at the center, and the slit 33a extends outward from the internal space. Is provided.
The biosensor system 30 is small in size, and is, for example, a handy type that the subject can hold with one hand, so that the user can use it by himself.

  As described above, according to the biosensor system 30 described above, the biosensor chip 10 can be easily attached and detached by grasping the protrusion 13 of the biosensor chip 10 without removing the protective cap 33 provided at the tip of the measuring device 31. be able to. Further, the biosensor chip 10 described above is attached to the drive means 32, and the biosensor chip 10 is driven by the drive means 32 to puncture, the sample is collected, and information on the sample is obtained via the detection electrodes 16a and 16b. Since the measurement can be performed in a short time and easily, the burden on the subject can be reduced.

  Examples of means for puncturing a subject with a puncture device include a spring and a motor. By using these drive mechanisms, the time required for puncturing can be shortened, and pain during puncturing can be reduced.

Next, a biosensor chip according to a second embodiment of the present invention will be described. 4A is a cross-sectional view showing a state in which an elastic body is attached to the tip of the biosensor chip, and FIG. 4B is an end view of the biosensor chip viewed from the B direction in FIG. 4A. In addition, the same code | symbol is attached | subjected to the site | part which is already common in the site | part already mentioned above, and the overlapping description is abbreviate | omitted.
The biosensor chip 10B has an elastic body 20 that covers the periphery of the puncture needle 12 that is a puncture device at the distal end portion 11a of the chip body 11.

The elastic body 20 can illustrate the cylindrical thing which has the through-hole 21 for forming sealed space in the center part, for example. The through hole 21 is larger than the outer diameter of the needle 12 because the needle 12 is inserted therethrough. In addition, the thickness of the elastic body 20 is set to a thickness that can reliably cover the tip of the needle 12. The material of the elastic body 20 is not particularly limited as long as it has elasticity, but rubber or sponge made of a polymer or a polymer such as silicone, urethane, acrylic, ethylene, styrene, etc., polyethylene, and polypropylene. Polyolefin such as polyethylene, polyester such as polyethylene terephthalate and polybutylene terephthalate, polytetrafluoroethylene, and fluororesin such as PFA which is a copolymer of perfluoroalkoxyethylene and polyfluoroethylene, can be used.
The rubber elastic body may be solid or hollow.

Note that the shape of the tip portion 11a of the chip body 11 corresponds to the shape of the bonding surface 22 of the elastic body 20, and the tip portion is preferably planar. For example, as shown in FIG. 4, when the cross-sectional shape of the chip body 11 is flat, a widened portion (not shown) is provided on the tip surface 11 a of the chip body 11 and the front surface of the elastic body 20 is bonded. It is better to do so.
In the biosensor chip 10B configured as described above, the shape of the contact surface 22 of the elastic body 20 provided at the tip of the chip body with the chip tip 11a is the same as the shape of the tip 11a of the chip body 11. Accordingly, the elastic body 20 can be securely attached and can be prevented from being detached or displaced when a pressing force is applied to the elastic body 20.

  The distal end surface 22 that is a surface in contact with the subject of the elastic body 20 is preferably coated with an adhesive or made of a material such as adhesive silicone or silicone gel. Thereby, the adhesiveness of the elastic body 20 and a test object can be improved. The inner peripheral surface of the through hole 21 is desirably made of a hydrophilic material, or at least the inner peripheral surface is subjected to a hydrophilic treatment. When the biosensor chip 10B is attached to the measuring device 31 and used, the size of the internal space (not shown) of the protective cap 33 of the measuring device 31 is set to a size that allows the elastic body 20 to move smoothly. There is a need to.

  In the biosensor chip 10B configured as described above, since the elastic body 20 is provided at the distal end portion 11a of the chip body 11, the puncture needle 12 protruding from the distal end portion 11a of the chip body 11 is protected, and the user Can be protected. Further, when the biosensor chip 10B is driven by the driving means 32 of the measuring device 31, the puncture needle 12 is extracted from the subject by the restoring force of the elastic body 20, and the sample flowing out from the puncture port is removed from the elastic body 20. Can be reliably collected. In addition, since the puncture needle 12 covers the tip of the elastic body 20 so as not to protrude from the distal end surface of the elastic body 20 when it is discarded after use, it can be disposed of safely and appropriately.

  The biosensor chip of the present invention is not limited to the above-described embodiments, and appropriate modifications and improvements can be made.

  As described above, in the biosensor chip according to the present invention, since the protrusion provided on the chip body protrudes from the slit of the protective cap provided at the tip of the measuring device, measurement is performed by gripping this protrusion. The biosensor chip can be easily attached to and detached from the measuring device without removing the protective cap of the device.

(A) is a front view which shows the state which mounted | wore the measuring apparatus with the biosensor chip concerning 1st Embodiment of this invention. (B) is the side view seen from the B direction in FIG. 1 (A). (A) is sectional drawing of the biosensor chip concerning 1st Embodiment. (B) is an end face of the biosensor chip viewed from the B direction in FIG. It is a block diagram of a biosensor system. (A) is sectional drawing of the biosensor chip concerning 2nd Embodiment. (B) is an end face of the biosensor chip viewed from the B direction in FIG. (A) is a perspective view which shows the conventional biosensor chip. (B) is an exploded perspective view showing a conventional biosensor chip.

Explanation of symbols

DESCRIPTION OF SYMBOLS 10 Biosensor chip 11 Chip body 11a Tip part 12 Puncture needle (puncture device)
13 Protrusion 16a, 16b Detection electrode 20 Elastic body 22 Adhesive surface 30 Biosensor system 31 Measuring device 32 Driving means 33 Protective cap 33a Slit

Claims (5)

A biosensor chip that samples a sample by driving a puncture device in a measurement device,
A biosensor chip characterized in that a protrusion is provided which protrudes outward from a slit of a protective cap attached to the tip of the measuring device and is movable.   The biosensor chip according to claim 1, wherein the protrusion is provided integrally with the chip body.   The biosensor chip according to claim 1, wherein an elastic body that covers the periphery of the puncture device is provided at a tip portion of the chip body.   The biosensor chip according to claim 3, wherein the shape of the tip of the chip body corresponds to the shape of the contact surface of the elastic body with the tip of the chip. A biosensor chip according to any one of claims 1 to 4, and a measuring device for attaching the biosensor chip interchangeably,
The measurement device has a slit provided at the tip of the measurement device, to which the protrusion of the biosensor chip can move, and a protective cap that protects the puncture device, and a drive that punctures the specimen by driving the chip body And a biosensor system.

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