JP2007101482A - Measuring tip and analytical method therefor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a measuring tip and its analytical method, wherein both visual qualitative analysis and quantitative values is achieved. <P>SOLUTION: The measuring tip 1 is provided with a substrate 2; a sample inlet part 5; a channel 6 on the substrate 2; a filter 7; a reagent region 3; and a substrate cover 8. The sample inlet part 5 is arranged at an end face of the substrate 2, and the inlet part 5, the channel 6, the filter 7, and the reagent region 3 are linked via the channel 6. The filter 7 is arranged between the sample inlet part 5 and the reagent region 3. The reagent region 3 has both an enzyme, which reacts at least with a substrate, and a color reagent which reacts with a reactant of the enzyme, and the sections of the substrate 2 that are not involved in reactions of the substrate have at least one or more collation color chart 4. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a measuring chip used for analyzing components in body fluids such as blood and an analysis method thereof.

  The measurement chip is used to measure the presence / absence and amount of albumin, glucose, leukocytes, occult blood, pH, specific gravity, creatinine, etc. in body fluids such as blood. The conventional measuring chip visually compares the color of the reagent area after applying the liquid with a reference color chart for judging the level or amount of the test result. In the color chart, the color tone of the basic density judgment rank is printed at regular rank intervals. The color chart is a chart paper different from the measurement chip, or is attached or attached to the measurement chip storage means (see, for example, Patent Document 1).

  FIG. 10 shows a conventional measurement chip in which a color area for comparison is formed on the measurement chip (see, for example, Patent Document 2). As shown in FIG. 10, two comparison color areas 101 and a reagent area 102 are configured on the substrate 100, and each of the two comparison color areas 101 is a reagent area 102 indicating the presence / absence result. The maximum amount of color that can appear, or the darkest color. One or more drops of the test solution is applied to the reagent area 102, and the resulting color in the reagent area 102 is compared with the color of each color area 101 for comparison to visually determine the presence / absence of the test substance. is doing. Here, the reagent region 102 and the two collation color regions 101 are placed in close proximity.

In a conventional measuring chip analysis method, an image of a test piece including a reagent region formed on a part of the test piece is picked up by a color image sensor, and the reflectance for each measurement wavelength in the reagent portion is obtained from a plurality of color signals obtained. In addition, the calibration curve prepared in advance is corrected by using the same reflectivity of the part of the test piece where the reagent area is not formed as the standard reflection part provided on the measurement chip. The measurement chip is analyzed using the calibration curve (for example, Patent Document 3).
Japanese Patent Laid-Open No. 11-326320 JP 2001-264327 A JP 07-005110 A

  However, in the conventional measuring chip, the reagent region and the collation color chart must be visually determined, and no matter how discontinuous the collation color chart is used, the quantitative determination with high resolution is possible. The value could not be obtained. In addition, when the collation color chart is separated from the measurement chip, many collation color charts are attached to the measurement chip storage container, so fading occurs due to exposure to the atmosphere, and accurate analysis is performed. I couldn't.

  A measurement chip with a comparison color area on the measurement chip also determines the presence / absence of the analyte in the comparison color area, and can provide a qualitative analyte concentration determination and quantitative value. There wasn't.

  In the conventional analysis method of the measurement chip, the correction of the reflection intensity due to the difference in illumination at the time of measurement is performed at the standard reflection part on the measurement chip. An image sensor is a collection of microsensors and microcolor filters, and the characteristics of each sensor are different, so quantitative analysis with high accuracy and high reproducibility could not be performed.

  The present invention has been made to solve the conventional problems, and an object of the present invention is to provide a measurement chip capable of obtaining a qualitative analysis by visual inspection and a quantitative value and an analysis method thereof.

  The measurement chip of the present invention has a reagent area on a substrate, a collation color chart for comparing with the color corresponding to the analyte concentration in the reagent area, and a reference color formed in or near the collation color chart. It has the structure which was made.

  With this configuration, the user can recognize the qualitative analyte concentration by visual observation, and when the measurement chip is photographed with a color image sensor, it can also recognize the quantitative analyte concentration after correcting for non-uniform illumination. It becomes.

  The measurement chip of the present invention has a configuration in which symbols associated with the characteristics of the measurement chip are written on the back surface of the substrate.

  With this configuration, the user can recognize the qualitative analyte concentration by visual observation, and when the measurement chip is photographed with a color image sensor, information such as the overall pattern of the measurement chip is given and quantitative analyte concentration is given. Can also be recognized.

  The measuring chip of the present invention has a configuration in which the reference color chart is continuously displayed with respect to the analyte concentration.

  With this configuration, the user can recognize the visually qualitative analyte concentration with higher accuracy.

  The measurement chip of the present invention comprises a substrate, a sample introduction part, a flow path having at least a bottom part and two or more side walls on the substrate, a filter, a reagent region, and a substrate cover, and the sample introduction part. Is on the end face of the substrate, and the sample introduction part, the flow path, the filter and the reagent region are connected via the flow path, and the filter is between the sample introduction part and the reagent region, The reagent region has at least an enzyme that reacts with a substrate and a color reagent that reacts with a reaction product of the enzyme, and the portion of the substrate that does not participate in the reaction has a configuration in which at least one reference color chart is formed. Have.

  With this configuration, the measurement chip can be used without the user touching a sample such as blood, and infection can be prevented.

  In the measuring chip analysis method of the present invention, the measuring chip includes a reference color chart for comparing at least a reagent region on the substrate and a color corresponding to the analyte concentration of the reagent region, The measurement chip is photographed with a color imaging device, a calibration curve with the analyte concentration is created from the color determination of the collation color chart from the captured image, and the analyte concentration is compared with the color determination of the reagent region. It has the structure made to obtain.

  With this configuration, the quantitative analyte concentration can be recognized when the user photographs the measurement chip with the color image sensor.

  In the measuring chip analysis method of the present invention, the measuring chip includes at least a reagent region on a substrate, a collation color chart for comparing the color corresponding to the analyte concentration in the reagent region, and the collation. The color density of the reference color chart including the reference color inside or near the color chart, the measurement chip photographed with a color imaging device, and corrected by the color judgment of the reference color from the captured image, and the analyte concentration and The calibration curve is prepared, and the analyte concentration is obtained by comparison with the color determination of the reagent region.

  With this configuration, when the user photographs the measurement chip with the color image sensor, the quantitative analyte concentration can be recognized after correcting for nonuniform illumination.

  In the measuring chip analyzing method according to the present invention, the color image pickup device is a mobile phone with a camera.

  With this configuration, when the user is a patient by using the communication function, the user's state is automatically shared with a doctor or the like.

  The present invention includes a comparison color chart for comparing at least a reagent region on a substrate with a color corresponding to the concentration of the analyte in the reagent region, and photographing the measurement chip with a color imaging device. By creating a calibration curve with the analyte concentration from the color determination of the collation color chart from the captured image and obtaining the analyte concentration by comparing with the color determination of the reagent region, the user can A measuring chip capable of recognizing a qualitative analyte concentration by visual observation and having an effect of recognizing a quantitative analyte concentration when the measuring chip is photographed with a color imaging device, and an analysis method thereof. It is.

  Hereinafter, a measurement chip and an analysis method thereof according to an embodiment of the present invention will be described with reference to the drawings.

  The measurement chip of the first embodiment of the present invention is shown in FIG. FIG. 1A is a cross-sectional view taken along the alternate long and short dash line (a) in the top view, and similarly, (b) is a cross-sectional view taken along the alternate long and short dash line (b) in the top view, and FIG. It is sectional drawing in a dashed line (c).

  In FIG. 1, a measuring chip 1 includes a reagent region 3 formed on the surface of a substrate 2, a sample introduction unit 5 and a channel 6 for introducing an analyte to the reagent region 3, and a filter 7 in the channel 6. And a plurality of collation color charts 4 corresponding to the analyte concentration steps in the analyte liquid, and a substrate cover 8 that defines the flow path 6 and covers the whole. The size of the measuring chip 1 is preferably a square or rectangle having a side of 20 mm or more, and more preferably a rectangular parallelepiped having a short side of 20 mm and a long side of 50 mm.

  The substrate 2 is made of a polymer plastic material such as polystyrene, polyester, polyvinyl chloride, polycarbonate, polypropylene, or a synthetic resin, and has a hardness that cannot be easily bent, and is a flat plate. The thickness is about several mm, preferably 1 to 3 mm. The substrate cover 8 is also made of the same material as that of the substrate 2, but has a material and thickness that are almost transparent to visible light. The thickness is 1 mm or less, preferably 0.02 to 0.2 mm.

  The groove of the channel 6 is formed by extruding the substrate 2, cutting or thermal processing of the substrate 2, and the like. The width of the channel 6 is about 5 mm or less, and the height is about 0.1 mm to 1 mm. Desirably, the width is 2 mm and the height is 0.2 mm. The length is not limited, but is preferably about 10 mm.

  The collation color chart 4 is formed by offset printing, gravure printing, letterpress printing, screen printing, thermal transfer printing, laser printing, ink jet printing, etc., such as paint, ink, pigment, and paint. The size of the collation color chart 4 is preferably a square or a rectangle with a side of 3 mm or more.

  The filter 7 is made of a fibrous material such as glass fiber or non-woven cloth, and is configured in a mesh shape. Depending on the fiber spacing, it is possible to capture components in the liquid to be analyzed that are larger than the spacing. The components to be captured are components that interfere with the reaction between the analyte and the reagent in the reagent region 3 and components that have a color other than the analyte. The fiber spacing is about several μm to several tens of μm, preferably 1 to 5 μm. The length of the flow path 6 in the flow direction is about several mm, preferably 1 to 5 mm.

  The operation of the measurement chip configured as described above will be described below by taking blood as an analyte and glucose as an analyte as an example.

  First, a user (not shown) uses a lancet (not shown) to exude blood, which is the liquid to be analyzed, from the fingertip or the like. The sample introduction part 5 of the measuring chip 1 is brought into contact with the exuded blood. The blood flows from the sample introduction part 5 through the flow path 6 to the reagent region 3 by capillary action. On the way, the blood cell component in the blood is captured by the filter 7, and only the plasma component reaches the reagent region 3.

  Enzyme colorimetric enzymes and chromogens known to those skilled in the art are applied to the reagent region 3 by coating, sticking or printing on the substrate 2, or coating, printing, impregnation on filter paper, film or other carrier, or It is installed on the substrate 2 in an integrated state by kneading. The size of the reagent region 3 is preferably a square or rectangle with a side of 3 mm or more. A color for controlling the coloration of the reagent region can be formed below the reagent region 3 and the substrate 2 by the same method as that for the collation color chart 4. However, the material selected from materials that do not react with the reagent in the reagent region 3 is different from that in the collation color chart 4.

  The blood, which is the liquid to be analyzed, that has reached the reagent region 3 reacts with glucose as the analyte and the enzyme in the reagent region 3 with glucose + oxygen + water- (glucose oxidase) → gluconic acid + hydrogen peroxide. Furthermore, the reaction product and chromogen react with another enzyme, hydrogen peroxide + chromogen-(peroxidase) → hydrogen peroxide + quinone dye. By this series of reactions, the reagent region 3 shows a color corresponding to the concentration of glucose as an analyte.

  Instead of glucose oxidase, pyranose oxidase can also be used. Instead of peroxidase, hemoglobin, iron thiocyanate, iron ferrocyanide, potassium iodide, ammonium molybdate, and the like can be used.

  The chromogen may be any chromogen that absorbs in the visible region, and o-anisidine, benzidine, o-tolidine, tetramethylbenzidine, leuco chromogen, 4-aminoantipyrine and phenol or its derivatives Or a Trinder reagent combining aniline derivatives can be used.

  By comparing this coloration with the matching color chart 4, the user can visually determine the qualitative value of the glucose concentration. Figure 8 shows the details of the matching color chart. In FIG. 8, 41 is a color chart portion, and 43 is a corresponding analyte concentration.

  Here, the corresponding analyte concentration 43 of the collation color chart 4 is a plurality of collation color charts at a step with a threshold value that can be used to determine a hyperglycemic or hypoglycemic state that requires subsequent treatment. You can also. Thus, the user can visually determine whether the glucose concentration is in a hyperglycemic state where prompt insulin intake is necessary or a hypoglycemic state where immediate sugar intake is necessary.

  Next, the quantitative analysis method of the analyte concentration is explained in detail. The analyte concentration is determined by comparing the calibration curve obtained from the matching color chart 4 with the coloration of the reagent region 3.

  Fig. 6 shows the configuration of the measurement chip analysis method. In FIG. 6, 81 is an illumination means, 82 is a color image sensor, 83 is an analyzer, and 84 is a display / input device. Incident light from the substrate 1 is reflected light from the substrate 1 including the reagent region 3 and the matching color chart 4 of some illumination means 81 such as sunlight or indoor illumination light.

  In a state where the liquid to be analyzed reaches the reagent region 3 and the reaction by the analyte has sufficiently progressed, the measurement chip 1 is photographed with a color imaging device 82 having a transmission means with the analyzer 83. The color image sensor 82 can be a semiconductor device such as a CCD or CMOS sensor. In the photographed image, the reagent region 3 and the collation color chart 4 and the reference color portion attached to each are shown.

  In the following, an example of the case where a CCD is used as the color image sensor 82 will be described as an analysis method of this image.

  A color CCD has a plurality of photodiodes arranged in a matrix, and a red, green, or blue color filter is usually placed on each photodiode. Some use yellow, magenta, and cyan, which are complementary colors. In the case of three primary colors, the color filters are arranged in groups of 9 photodiodes (3 x 3), 2 red and blue color filters in the vertical and horizontal directions, and 5 green color filters at the center and four corners. in use. The electrical signal output of each photodiode is proportional to the light intensity of the incident light after passing through the color filter.

  The first stage of image analysis in the analyzer 83 is to recognize the measurement chip 1 and the reagent region 3 and the plurality of collation color charts 4 therein. This is performed by edge detection between each recognition unit and the substrate 2. Matching with the whole pattern stored in advance is performed, the magnification at the time of photographing is determined, each recognition unit is determined, and the photodiode address on the color CCD correlated with each recognition unit is determined.

  Here, the entire pattern identification number is written on the surface of the measuring chip 1 opposite to the surface where the reagent region 3 is located, and this is input to the display / input device 84 having the analyzing device 83 and some transmission means for analysis. An overall power pattern can be given. FIG. 9 shows the surface of the measuring chip 1 opposite to the surface where the reagent region 3 is present. In FIG. 9A, 9 is an overall pattern recognition number.

  Alternatively, the whole identification number can be a one-dimensional or two-dimensional bar code, photographed by the color imaging device 82, and recognized to give a whole pattern to be analyzed. In FIG. 9B, 9 is an example of a two-dimensional barcode. Furthermore, it is also possible to add information such as the type of analyte other than the overall pattern identification, the required analysis accuracy, and the method of transmitting the analysis result to the user.

  In the second stage, the color of each recognition unit is judged. Each recognition unit corresponds to at least a plurality of photodiodes for each color, and calculates the average of the electrical signal outputs of the same color and the median of the frequency distribution. As a result, an electrical signal output of each color (red = R, green = G, blue = B) is obtained. This is generally called RGB output value. This operation is repeated for each recognition unit.

  The third step is to create a calibration curve with the glucose concentration based on the results of the second step. As a result of the examination by the inventor, among the RGB outputs, there was a correlation between the R and G outputs and the glucose concentration, and a calibration curve was created for these two. In FIG. 11, (a) shows the relationship between R output and glucose concentration, and (b) shows the relationship between G output and glucose concentration.

As can be seen from FIG. 11, when the glucose concentration increases, the R output and the G output tend to decrease.
[Glucose concentration] = A × exp (−B × [R output or G output])
A calibration curve can be created in the form of

  The fourth stage is a color comparison between the calibration curve from the collation color chart 4 and the reagent area 3 in the third stage. The color determination similar to the second step performed for the collation color chart 4 is performed for the reagent region 3, and the result is substituted into the calibration curves for the R output and the G output to determine the glucose concentration in the blood. The analyte concentration is stored in a storage means in the analyzer 83 in a state that can be redisplayed later along with the shooting time, user event information, and the like, and is stored in the display / input device 84 having the analyzer 83 and some transmission means. Displayed and recognized by the user.

  The previous third and fourth stages can be modified as follows.

  The third stage that can be changed is the processing of RGB output values. There are three independent variables in the RGB output value, representing only the ratio of each color. This is converted to the color space. Color space here means lightness, saturation, and hue. Various expression methods have been proposed as a color space display method, which is called a color system. Examples of the color system include an XYZ (Yxy) color system, an L * a * b color system, and an L * C * h color system.

  Here, the conversion method is shown taking the L * C * h color system as an example. In conversion, the standardized RGB output values are once converted into the XYZ color system. Furthermore, the XYZ color system is converted to the L * a * b color system, and finally the L * C * h color system is converted. In the L * C * h color system, L * represents lightness, C * represents saturation, and h represents hue angle. FIG. 7 shows a color chart of the L * C * h color system.

  The fourth step that can be changed is the creation of a calibration curve from the matching color chart 4 and the color comparison of the reagent region 3. The color judgment result after conversion of each collation color chart is mapped in the color system. The mapping is arranged in the three-dimensional space consisting of brightness, saturation and hue angle shown in Fig.7. Furthermore, the color determination result after conversion of reagent region 3 is also applied to the previous mapping in the color system. The analyte concentration of the analyte can be determined by a ratio distribution with adjacent known concentrations on the fitted mapping.

  The ratio distribution can use two dimensions of lightness and saturation, or two dimensions of lightness and hue angle, and it is possible to use one of these or two averages. Preferably, of the two types of two dimensions, the dimension having the largest adjacent interval of known density is used.

  Further, the color imaging device 82, the analysis device 83, and the display / input device 84 may be a camera-equipped mobile phone in which these are integrated. By using a camera-equipped mobile phone, in addition to the above operations, an external communication function is added. For example, when the user is a diabetic patient, communication with a doctor is possible through the communication function, or automatically. It is also possible to record the analysis results in a doctor's database.

  According to the measurement chip and the analysis method of the first embodiment of the present invention as described above, the collation color chart is provided on the measurement chip, the measurement chip is photographed by the color imaging device, and the pattern recognition and the collation color chart are performed. A calibration curve with the analyte concentration is obtained from the sample, and color comparison with the reagent area can be performed. This allows the user to visually recognize the qualitative analyte concentration and to perform quantitative analysis by photographing the measurement chip. Concentration can be measured with a single measuring chip.

  Furthermore, by adding a unique number or bar code to the measuring chip and inputting it to the analyzer, the entire pattern of the measuring chip and other information specific to the measuring chip can be automatically given to the analyzer. .

  Furthermore, by using a camera-equipped mobile phone for shooting, it is not only possible to perform shooting, analysis, display, and storage with a single device. If the user is a patient by using the communication function, doctors, etc. It becomes possible to automatically share the user status.

  Next, a measurement chip and an analysis method according to the second embodiment of the present invention will be described with reference to the drawings. In the second embodiment of the present invention, only differences from the first embodiment of the present invention will be described in detail.

  The detailed configuration of the collation color chart 4 is shown in FIG. In FIG. 3, the collation color chart 4 is composed of a color chart portion 41 and reference color portions 42a, 42b, and 42c used for the previous visual determination. The reference color portion 42a is a red reference color, 42b is a green reference color, and 42c is a blue reference color. The positions of the reference collar portions 42a, 42b, and 42c are not limited to the arrangement shown in FIG. 3, but are desirably positions that do not affect visual observation.

  Similarly, the detailed structure of the reagent region 3 is shown in FIG. In FIG. 4, reference collar portions 31a, 31b, 31c are arranged around the reagent region 3. The reference color portion 31a is a red reference color, 31b is a green reference color, and 31c is a blue reference color. The positions of the reference collar portions 31a, 31b, and 31c are not limited to the arrangement shown in FIG. 4, but are desirably positions that do not affect visual observation.

  The reference color part has three primary colors of red, green, and blue, but other primary colors, complementary colors, and the number of colors can be changed. Alternatively, the reference brightness, saturation, and hue may be used.

  Incident light from the substrate 1 is reflected light from the substrate 1 including the reagent region 3 of some illumination means 81 and the collation color chart 4 such as sunlight and indoor illumination light, and the state of the illumination means 81 and the illumination means 81. Depending on the positional / angular relationship of the substrate 1 and the color image sensor 82, it differs for each photographing, and the illumination light is not uniform within the surface of the substrate 1, and the conditions are different.

  For this reason, in the color determination of the collation color chart 4 and the reagent region 3, there is a possibility that the accuracy is lowered due to a difference in illumination light at each color determination place. Therefore, the color judgment is corrected using the reference color part. A case where the reference color portions are red, green, and blue will be described as an example of the color determination correction method.

  First, as in the case of the collation color chart part and reagent area, the reference color part is recognized and the color is determined for each color reference part, and each RGB output value is calculated. Originally, if the lighting conditions on the entire surface of the measuring chip are uniform, the RGB output values of the color reference portion at each color judgment location should be the same. However, if the lighting conditions are not uniform, the RGB output values at each color judgment location will be different. Fig. 12 (a) shows an example of the output before correction and the glucose concentration when the illumination is uneven. In FIG. 12A, the slope changes from the glucose concentration of 200 mg / dl.

  Therefore, each reference color chart 4 is corrected using the reference color unit 31. That is, each RGB output value that should be originally obtained in each of the reference color sections 31 is compared with the RGB output value that is actually obtained, and the RGB output of the matching color chart 4 is corrected with the decrease or increase ratio. This correction is performed for all the matching color chart sections and reagent areas, and a calibration curve is created from the matching color chart 4 using the corrected values, and the blood glucose concentration can be determined by color comparison with the reagent areas. .

  FIG. 12B shows an example of the relationship between the corrected glucose concentration and the output value. As indicated by the downward arrow in FIG. 12B, a calibration curve with reduced errors can be created as a result of correction based on the color determination result in the reference color portion.

  According to the measurement chip and the analysis method of the second embodiment of the present invention as described above, the reference chip is provided in each of the collation color chart and the reagent area on the measurement chip, so that the measurement chip is connected to the color imaging device. It is possible to correct a decrease in the accuracy of color determination due to non-uniform illumination light when shooting.

  Next, a measurement chip according to a third embodiment of the present invention will be described with reference to FIGS. In the third embodiment of the present invention, only differences from the previous embodiments of the present invention will be described in detail.

  FIG. 2 shows a measurement chip according to the third embodiment of the present invention. In the measurement chip 1 of FIG. 2, the same symbols as those of the measurement chip of FIG. What is different from FIG. 1 is a collation color chart 4, and details of the collation color chart 4 are shown in FIG. In the collation color chart 4 of FIG. 5, the color chart part 41 is a continuous chart, and the reference color part 42 is arranged in a certain step. Corresponding analyte concentration 43 is also arranged in a certain step, and display between steps is performed by step display 44.

  In FIG. 5, the reference collar portion 42 is arranged so as to coincide with the corresponding analyte concentration 43, but it may be more or less. Further, the reference collar portion 42 may be installed outside the vicinity of the color chart 41.

  A comparison of visual coloration with a reagent region (not shown) is performed with the matching color chart 4. In addition, the measurement chip 1 is photographed by a color image sensor (not shown), and color determination is performed by an analyzer (not shown).

  The color determination of the matching color chart 4 is performed at a plurality of locations on the matching color chart 4. The multiple locations are preferably performed in the vicinity of the reference collar portion 42. A calibration curve is created from the collation color chart 4 after correction by color determination of the reference color portion 42, and blood is also obtained by color comparison with the reagent region 3 after correction by color determination of the reference color portion. Glucose concentration is determined.

  According to the measurement chip and the analysis method of the third embodiment of the present invention, the reference color portion is provided in each of the reagent regions by making the collation color chart on the measurement chip a continuous chart. As a result, the accuracy of visual recognition of the qualitative analyte concentration by the user can be increased.

  As described above, the measurement chip and the analysis method according to the present invention have the effect that the user can perform qualitative recognition of the analyte concentration by visual observation and quantitative recognition by photographing the measurement chip with one measurement chip. It is useful as a measurement chip and its analysis method used for analyzing components in body fluids such as blood.

The block diagram which shows the measurement chip | tip in the 1st Embodiment of this invention The block diagram which shows the measuring chip in the 3rd Embodiment of this invention Detailed view of collation color chart in the second embodiment of the present invention Detailed view of reagent region in the second embodiment of the present invention Detailed view of collation color chart in the third embodiment of the present invention The block diagram which shows the analysis method of the measuring chip in the 1st Embodiment of this invention Color chart of L * C * h color system in the first embodiment of the present invention Detailed view of collation color chart in the first embodiment of the present invention The back view of the measurement chip in the 1st embodiment of the present invention Configuration diagram showing a conventional measuring chip Graph showing the relationship between output and glucose concentration Graph showing the relationship between output before and after correction and glucose concentration

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Measurement chip 2 Substrate 3 Reagent area 4 Collation color chart 5 Sample introduction part 6 Flow path 7 Filter 8 Substrate cover 9 Whole pattern recognition number or two-dimensional barcode 31 Reference color part 41 Color chart part 42 Reference color part 43 Corresponding analyte Concentration 44 Step display 81 Illumination means 82 Color imaging device 83 Analytical device 84 Display / input device 100 Substrate 101 Comparison color region 102 Reagent region

Claims (8)

A reference color is formed on or in a reagent area, a matching color chart for comparing with a color corresponding to the analyte concentration in the reagent area, and a reference color in or near the matching color chart. Measuring chip. The measuring chip according to claim 1, further comprising a flow path for guiding the analyte to the reagent region. 3. The measuring chip according to claim 1, wherein a symbol associated with the characteristics of the measuring chip is marked on the back surface of the substrate. 4. The measuring chip according to claim 1, wherein the collation color chart is continuously displayed with respect to the analyte concentration. A substrate, a sample introduction part, a flow path having at least a bottom part and two or more side walls on the substrate, a filter, a reagent region, and a substrate cover, the sample introduction part being on an end surface of the substrate; The sample introduction part, the channel, the filter, and the reagent region are connected via the channel, the filter is between the sample introduction unit and the reagent region, and the reagent region reacts with at least a substrate. A measuring chip comprising an enzyme and a color reagent that reacts with a reaction product of the enzyme, and a portion of the substrate that does not participate in the reaction has at least one color matching chart. The measurement chip includes at least a reagent region on a substrate and a matching color chart for comparing the color corresponding to the analyte concentration of the reagent region, and the measurement chip is photographed and captured by a color imaging device. A method for analyzing a measuring chip, wherein a calibration curve with the analyte concentration is created from color determination of the collation color chart from an image, and the analyte concentration is obtained by comparison with color determination of the reagent region . The measurement chip includes at least a reagent area on a substrate, a collation color chart for comparing with a color corresponding to the analyte concentration in the reagent area, and a reference color in or near the collation color chart, The measurement chip is photographed with a color imaging device, a calibration curve with the analyte concentration is created from the color determination of the reference color chart corrected by color determination of the reference color from the captured image, and the color of the reagent region A method for analyzing a measuring chip, wherein the analyte concentration is obtained in comparison with a determination. 8. The method for analyzing a measuring chip according to claim 6, wherein the color imaging device is a camera-equipped mobile phone.

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