JP2006254918A5 - - Google Patents

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JP2006254918A5
JP2006254918A5 JP2006118714A JP2006118714A JP2006254918A5 JP 2006254918 A5 JP2006254918 A5 JP 2006254918A5 JP 2006118714 A JP2006118714 A JP 2006118714A JP 2006118714 A JP2006118714 A JP 2006118714A JP 2006254918 A5 JP2006254918 A5 JP 2006254918A5
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hemoglobin
surfactant
glycated
measuring
protease
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JP2006118714A
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JP2006254918A (en
JP4301523B2 (en
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次の１）〜３）の工程を同一反応槽中で行うことを特徴とする糖化ヘモグロビンのヘモグロビンに対する割合の測定方法：
１）被検液中のへモグロビンの定量
２）該ヘモグロビンのプロテアーゼ処理
３）フルクトサミンオキシダーゼを用いた糖化アミノ酸の定量 The method of measuring the ratio of glycated hemoglobin to hemoglobin, wherein the following steps 1) to 3) are carried out in the same reaction vessel:
1) Determination of hemoglobin in test solution 2) Protease treatment of the hemoglobin 3) Determination of glycated amino acid using fructosamine oxidase プロテアーゼを金属イオン、プロテインA若しくはプロテインGの存在下作用せしめる請求項１記載の測定方法。 Protease metal ions, measurement method of claim 1, wherein the exerting presence of protein A or protein G. ヘモグロビンの定量及び糖化ヘモグロビンの定量を同一波長で行う請求項１または２に記載の測定方法。 The measuring method according to claim 1 or 2 for quantitative quantification and glycated hemoglobin hemoglobin at the same wavelength. プロテアーゼ濃度が５００〜５０００００ＰＵ/ｍｌである請求項１〜３のうちいずれか１項に記載の測定方法。 Measurement method according to any one of claims 1 to 3 protease concentration is 500 ~500000PU / ml. ヘモグロビンの定量時に、少なくとも硫酸基を含有する界面活性剤、及び/又は非イオン性界面活性剤、及び/または両イオン性界面活性剤を添加し、かつヘモグロビン及び糖化ヘモグロビン定量時のｐＨが５．０〜９．５である請求項１〜４のうちいずれか１項に記載の測定方法。 When quantifying hemoglobin, a surfactant containing at least a sulfate group and / or a nonionic surfactant and / or a zwitterionic surfactant is added, and the pH when quantifying hemoglobin and glycated hemoglobin is 5. It is 0-9.5, The measuring method of any one of Claims 1-4. 次の１）〜３）を同一反応槽中で行うことを特徴とする糖化ヘモグロビンのヘモグロビンに対する割合の測定方法。A method for measuring a ratio of glycated hemoglobin to hemoglobin, wherein the following 1) to 3) are carried out in the same reaction tank.
１）被検液中のヘモグロビンを、少なくとも硫酸基を含有する界面活性剤、及び/又は非イオン性界面活性剤、及び/または両イオン性界面活性剤の存在下、ｐＨ５．０〜９．５の範囲内で定量する工程、1) Hemoglobin in a test solution is pH 5.0 to 9.5 in the presence of a surfactant containing at least a sulfate group and / or a nonionic surfactant and / or a zwitterionic surfactant. Quantifying within the range of
２）被検液に、５００〜５０００００ＰＵ/ｍｌのプロテアーゼを添加し、工程１）のシグナル変化が一定になるようにする工程、2) A step of adding 500 to 500,000 PU / ml protease to the test solution so that the signal change in step 1) becomes constant,
３）上記２）の反応液に、ｐＨ５．０〜９．５の範囲内でフルクトサミンオキシダーゼを作用させて糖化ヘモグロビンを定量する工程。3) A step of quantifying glycated hemoglobin by allowing fructosamine oxidase to act on the reaction solution of 2) within a range of pH 5.0 to 9.5. ヘモグロビン定量試薬、プロテアーゼおよびフルクトサミンオキシダーゼを含有してなる糖化ヘモグロビンのヘモグロビンに対する割合測定用組成物。 A composition for measuring a ratio of glycated hemoglobin to hemoglobin, comprising a hemoglobin quantitative reagent, a protease, and fructosamine oxidase . 金属イオン、プロテインA若しくはプロテインGを含有してなる請求項７記載の糖化ヘモグロビンのヘモグロビンに対する割合測定用組成物。 The composition for measuring a ratio of glycated hemoglobin to hemoglobin according to claim 7, comprising metal ion, protein A or protein G. ヘモグロビン定量試薬が少なくとも硫酸基を含有する界面活性剤、及び/又は非イオン性界面活性剤、及び/または両イオン性界面活性剤を含有し、かつ、ヘモグロビン定量試薬のｐHが５．０〜９，５である、請求項７又は８に記載の糖化ヘモグロビンのヘモグロビンに対する割合測定用組成物。 The hemoglobin quantitative reagent contains at least a surfactant containing a sulfate group, and / or a nonionic surfactant, and / or an amphoteric surfactant, and the hemoglobin quantitative reagent has a pH of 5.0 to 9 The composition for measuring a ratio of glycated hemoglobin to hemoglobin according to claim 7 or 8 . １）少なくとも硫酸基を含有する界面活性剤、及び/又は非イオン性界面活性剤、及び/または両イオン性界面活性剤、及び定量時のｐＨが５．０〜９．５になる緩衝液；1) A surfactant containing at least a sulfate group, and / or a nonionic surfactant, and / or a zwitterionic surfactant, and a buffer solution having a pH of 5.0 to 9.5 at the time of determination;
２）５００〜５０００００ＰＵ/ｍｌのプロテアーゼ；2) 500 to 500,000 PU / ml protease;
３）ケトアミンオキシダーゼ、定量時のｐＨが５．０〜９．５になる緩衝液、糖化ヘモグロビン定量用色素、及び少なくとも硫酸基を含有する界面活性剤、及び/又は非イオン性界面活性剤、及び/または両イオン性界面活性剤；3) a ketoamine oxidase, a buffer solution having a pH of 5.0 to 9.5 at the time of quantification, a glycated hemoglobin quantification dye, a surfactant containing at least a sulfate group, and / or a nonionic surfactant, And / or zwitterionic surfactants;
を含有する同一反応槽中でヘモグロビンの定量及び糖化ヘモグロビンの定量を行うための組成物。A composition for quantifying hemoglobin and glycated hemoglobin in the same reaction vessel containing
JP2006118714A 2006-04-24 2006-04-24 Glycated protein ratio measurement method Expired - Lifetime JP4301523B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2006118714A JP4301523B2 (en) 2006-04-24 2006-04-24 Glycated protein ratio measurement method

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JP2006118714A JP4301523B2 (en) 2006-04-24 2006-04-24 Glycated protein ratio measurement method

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JP2000020224A Division JP4010474B2 (en) 2000-01-28 2000-01-28 Glycated protein ratio measurement method

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JP2006254918A JP2006254918A (en) 2006-09-28
JP2006254918A5 true JP2006254918A5 (en) 2008-09-18
JP4301523B2 JP4301523B2 (en) 2009-07-22

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Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2541114T3 (en) * 2007-11-20 2015-07-16 Siemens Healthcare Diagnostics Inc. Methods for the detection of glycosylated hemoglobin
JP5515017B2 (en) * 2008-02-19 2014-06-11 国立大学法人神戸大学 Method for decoloring coloring components using protease for decolorization
JP2014102144A (en) * 2012-11-20 2014-06-05 Nipro Corp Glycosylated hemoglobin measuring kit and glycosylated hemoglobin measuring method
US20240027313A1 (en) * 2020-11-25 2024-01-25 Provigate Inc. Medium for separating and storing blood plasma or serum, method for producing the medium, device, kit, and method for measuring glycated protein
CN114184693B (en) * 2021-10-14 2023-10-13 重庆医科大学 Application of 4-hydroxyphenylacetic acid as marker in preparation of diagnosis kit for sepsis encephalopathy
CN114112587A (en) * 2021-11-25 2022-03-01 青岛汉唐生物科技有限公司 Preparation method of glycated albumin calibrator or quality control product

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