JP2005237617A - Body liquid collecting device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a body liquid collecting device especially for collecting bone marrow liquid from the oral cavity, jawbone, ilium bone, breast bone or the like that contain mesenchymal stem cells or blood stem cells or various types of body liquid containing bone marrow liquid, such as blood or the like, which is capable of preventing a medicine prestored in a body liquid pooling section from flowing reversely to the outside. <P>SOLUTION: A connector 130 is arranged between and coupled to an injection syringe 115 and an injection needle 120. The connector has a check valve 144 that introduces the collected bone marrow liquid or the like from the injection needle 120 to the injection syringe 115 but prevents the flow of the bone marrow liquid in the reverse direction. The device is so configured that at least the connector 130 and the injection syringe 115 are attachable and detachable. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a bodily fluid collecting device, and more particularly, a bodily fluid collecting device that collects bodily fluids such as bone marrow fluid containing somatic stem cells such as so-called mesenchymal stem cells and blood stem cells, and blood including such bone marrow fluid. About.

  In recent years, somatic stem cells such as mesenchymal stem cells have attracted attention as practical cells used in regenerative medicine. Mesenchymal stem cells have the ability to differentiate into many types of cells such as bone, cartilage, heart muscle, fat, and nerve, and when transplanted, promote the regeneration of many tissues. In addition to being able to be collected from adult bone marrow, mesenchymal stem cells are also present in periosteum, adipose tissue, amniotic membrane, placenta and the like (see, for example, Non-Patent Document 1).

  When somatic stem cells such as mesenchymal stem cells are administered to a patient, at least 100 to 30 million cells are required. In particular, in order to use mesenchymal stem cells for regenerative medicine, regeneration thereof is required. It is necessary to grow in large quantities while maintaining the ability. As a method for proliferating mesenchymal stem cells, a technique using basic fibroblast growth factor (bFGF) has been developed, and regenerative medicine can be performed even with a small amount of bone marrow fluid (0.5 ml) as a starting point. This is possible (Patent Document 1).

  Examples of methods for collecting somatic stem cells and progenitor cells such as bone marrow mesenchymal stem cells include collecting bone marrow fluid by injecting an injection needle into the periodontal region, and extracting after extraction of teeth containing bone marrow fluid derived from the oral cavity. A method of collecting blood is also conceivable. Conventionally, in order to collect bone marrow fluid from the oral cavity, jaw bone, iliac bone, sternum, etc., a general syringe has been used.

International Publication No. 02/22788 Pamphlet

JP 7-184991 A

JP 2002-276879 A

Yukio Kato and two others, “Characteristics of mesenchymal stem cells and application to regenerative medicine”, High-Tech Information, No. 141, China Technology Promotion Center, p. 27-34

  When collecting bodily fluids such as the above-mentioned bone marrow and blood containing bone marrow using a general syringe, various drugs such as anticoagulants such as heparin are held in advance in the syringe barrel where the collected bodily fluids are stored. (For example, refer to Patent Document 2 for examples of various drugs). However, if a force that pushes the piston is applied at the time of collecting body fluid, the medicine held in the syringe barrel and the collected stem cell-containing body fluid may flow backward and return to the oral cavity again. It had the problem that. In the technique described in Patent Document 2, umbilical cord blood collected using a three-way stopcock or the like is transferred to a bag, and a drug such as heparin is retained in the bag, so that the drug flows backward. There is no problem, but when the device becomes large and collects bodily fluids in the oral cavity, it is inconvenient.

  The present invention has been made in view of such problems, and in particular, various body fluids such as oral cavity containing mesenchymal stem cells, blood stem cells, etc., bone marrow fluid derived from iliac bones, blood containing bone marrow fluid, etc. An object of the present invention is to provide a bodily fluid collecting device in which a medicine previously held in a body fluid collecting portion does not flow back into the oral cavity when collecting.

  In order to solve the above problems, a bodily fluid collecting device according to the present invention is a bodily fluid collecting device that collects bodily fluids. The bodily fluid collecting device has an inflow hole through which bodily fluids flow in, and a storage space that stores bodily fluids that flow through the inflow holes. A body fluid reservoir configured to be able to be gradually increased by manual operation, and a collection hole to be applied to a site where the body fluid to be collected exists, and the volume of the storage space is gradually increased manually A bodily fluid collection part that forms a flow path through which the bodily fluid collected from the collection hole flows, an outflow hole of the bodily fluid collection part provided on a side different from the collection hole in the flow path, and the bodily fluid storage part A connector for connecting the inflow hole to the inflow hole, and the connector includes a backflow prevention unit that prevents body fluid from flowing from the outflow hole to the inflow hole but does not flow in the reverse direction. Fluid retention Bets are characterized by being composed detachably.

  By taking such a configuration, the body fluid once collected in the storage space at the time of body fluid collection and various drugs such as an anticoagulant previously held in the storage space are prevented from flowing back, and in particular, bone marrow derived from the oral cavity A body fluid collection process that is more comfortable for a person who collects body fluid such as fluid is realized, and it is suppressed that the collected body fluid flows backward and returns to the outside, and the efficiency of body fluid collection is reduced. The

  In order to discharge the collected body fluid to the outside of the apparatus, the connector and the inflow hole need to be detachable, but the body fluid collection part and the connector need not be detachable. There is no. In this case, the outflow hole of the body fluid collecting part is always connected to the connector, and the outflow hole may not be visible from the outside. As an example of the body fluid storage part, it is possible to use a syringe body including a cylinder body in which the inflow hole is formed at the tip and the manually operated piston, but is not limited thereto, for example, after being crushed once, It is also conceivable to use instruments made of various elastic bodies, elastic resins, etc., such as a spherical container whose internal volume can be gradually increased by manual operation.

  Further, in the case where the body fluid collection unit and the connector are configured to be detachable, an attachment / detachment method between the body fluid collection unit and the connector, and an attachment / detachment method between the body fluid storage unit and the connector, Can be different. For example, one may be a luer connection and the other a lock connection. For example, when a puncture needle is used in the body fluid collecting part, if it is preferable to remove the puncture needle for the time being, the connector and the puncture needle may be connected by a luer type. The connection method is not particularly limited, and it is effective in preventing erroneous connection by making the both attachment and detachment methods different. The body fluid collecting unit is not limited to the puncture needle, and can be used even if the tip is not needle-shaped, particularly when collecting bleeding after tooth extraction.

  According to the bodily fluid collection device of the present invention, since the backflow prevention unit is provided between the bodily fluid collection unit and the bodily fluid storage unit, for example, the medicine preliminarily held in the bodily fluid storage unit configured by a syringe barrel or the like is collected. There is an effect that it is possible to prevent the part from flowing back and returning to the outside.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.
Fig.1 (a) is a front view for demonstrating the structure of the bodily fluid sampling device of this Embodiment. As shown in the figure, the bodily fluid collection device 100 of the present embodiment includes an injection cylinder 115 including a cylinder main body 111 and a piston 112, an injection needle 120, and a connector 130. By manually operating the piston 112 in the backward direction, the syringe cylinder 115 gradually increases the body fluid storage space formed with the cylinder main body 111.

  In addition, as the connector 130 of this Embodiment, the thing similar to the rotary connector with a valve currently disclosed by patent document 3 (thing which reverse | turned the direction of the non-return valve) can be used, for example. FIG. 1B is a cross-sectional view of the connector 130. The connector 130 is a valve member in which a first connector member 141 provided with a lumen 131 into which a luer portion 113 provided at the tip of the cylinder body 111 is inserted, and a second connector member 142 include a check valve 144. The injection needle 120 can be attached to the luer part 132 of the second connector member 142. The valve member 143 (including the check valve 144) can be manufactured using an elastomer such as silicone rubber. For the connector 130 shown in the figure, the materials of the first connector member 141, the second connector member 142, the valve member 143, the valve member 143, etc., except that the direction of the check valve 144 is reversed. At a portion where the fringe 145 formed on the valve member 143 is sandwiched between the first connector member 141 and the second connector member 142. Details such as the provision of ribs (not shown in FIG. 1B) on the member 141 and the second connector member 142 are described in detail in the above-mentioned Patent Document 3, and the same applies to the present embodiment. However, detailed description is omitted here.

  As described above, in the bodily fluid collection device 100 of the present embodiment, by providing the check valve 144, bodily fluid such as bone marrow fluid collected from the injection needle 120 side flows into the syringe barrel 115 side. However, since it is configured not to flow in the reverse direction, for example, when a bodily fluid such as bone marrow fluid derived from the oral cavity is collected, the drug solution or the like previously held on the syringe barrel 115 side flows back into the oral cavity. There is no. Of course, the use of the bodily fluid collecting device of the present invention is not limited to collecting bodily fluids derived from the oral cavity.

(Modification)
Although the embodiments of the present invention have been described above, the content of the present invention is not limited to the specific examples described in the above embodiments. For example, the following modifications may be considered. it can.

  (1) In the above embodiment, since the instrument used when both are connected by the luer type is used as the tube main body 111 and the injection needle 120, the shapes of the first connector member 141 and the second connector member 142 of the connector 130 are used. Although the shape is adapted to the luer connection, the connection method between the syringe 115 and the connector 130 and between the connector 130 and the injection needle 120 may be different. For example, when it is desired to remove the injection needle 120 with the body fluid collected in the syringe 115 serving as a body fluid storage unit being securely removed, for example, the connector 130 and the injection needle 120 are connected by a luer method. In addition, the connection between the connector 130 and the syringe 115 can be a lock system.

  (2) The configuration of the backflow prevention unit provided in the connector 130 in the above embodiment is not limited to the configuration of the check valve 144. For example, although the flow path is normally closed, the collected body fluid The present invention can be applied to any plate-like member that moves or opens and closes in one direction when the water flows in, or any other backflow prevention device that can be used for medical purposes. Of course, it is preferable to optimize based on conditions such as the internal volume of the body fluid reservoir, such as the size, thickness, and material of the plate-like member and the check valve 144.

  The present invention can be applied to, for example, a body fluid collection device that collects various body fluids such as bone marrow fluid derived from the oral cavity.

(A) It is a front view for demonstrating the structure of the bodily fluid sampling device in embodiment of this invention. (B) It is sectional drawing which shows an example of a structure of the connector 130. FIG.

Explanation of symbols

DESCRIPTION OF SYMBOLS 100 Body fluid collecting device 111 Cylinder main body 112 Piston 113 Luer part 115 Injection cylinder 120 Injection needle 130 Connector 131 Lumen 132 Luer part 133 Valve accommodating part 141 1st connector member 142 2nd connector member 143 Valve member 144 Check valve 145 Fringe

Claims (5)

In a bodily fluid collection device for collecting bodily fluids,
A bodily fluid storage section configured to have an inflow hole into which bodily fluid flows, and the volume of the storage space for storing bodily fluid flowing in from the inflow hole can be gradually increased by manual operation;
Body fluid collection having a collection hole to be applied to a site where the body fluid to be collected exists, and a flow path through which the body fluid collected from the collection hole flows when the volume of the storage space is gradually increased manually And
A connector for connecting the outflow hole of the bodily fluid collection part provided on the side different from the collection hole in the flow path and the inflow hole of the bodily fluid storage part;
The connector includes a backflow prevention unit that prevents body fluid from flowing from the outflow hole to the inflow hole but does not flow in the reverse direction, and the connector and the body fluid storage unit are configured to be detachable. A bodily fluid collection device characterized by the above. The bodily fluid collecting device according to claim 1, wherein the bodily fluid storage unit includes an injection cylinder including a cylinder main body having the inflow hole formed at a distal end portion and the manually operated piston. The screw | thread is formed in the surrounding part of the inflow hole of the said bodily fluid storage part, It is comprised so that attachment or detachment is possible by screwing between the connection parts of the said connector. The bodily fluid collection device described in 1. The body fluid collection device according to any one of claims 1 to 3, wherein the body fluid collection unit and the connector are configured to be detachable. The humor sampling device according to claim 4, wherein a detaching method between the humor collecting part and the connector is different from a detaching method between the humor storing part and the connector.

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