JP2005088111A - Manufacturing method of micro-reactor having separation/extraction mechanism - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a manufacturing method of a micro-reactor, quickly manufacturing the micro-reactor having an extraction/separation mechanism in which two fine passages intersect each other at a very small angle at low cost. <P>SOLUTION: This manufacturing method of the micro-reactor having the separation/extraction mechanism uses a metal mold formed so that a projection for forming the fine passage 12 is formed at the end part of the split metal mold partly parallel to the end face in one split metal mold A of adjacent split metal molds, a projection for forming the fine passage 22 is formed at the end part of the split metal mold partly parallel to the end face in the other split metal mold B of adjacent split metal molds, and when the split metal mold A and the split metal mold B are assembled, the above part of the projection for forming the fine passage 12 and the above part of the projection for forming the fine passage 22 are adjacent to each other, thereby forming a recessed part corresponding to a fine cavity in a synthetic resin plate. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

The present invention relates to a method of manufacturing a microreactor having a separation / extraction mechanism in which a fine flow path for transferring a sample or the like between synthetic resin plates, a storage tank, a reaction mechanism, a detection mechanism, a waste liquid storage tank, and the like are formed.

Specifically, microreaction devices such as chemistry and biochemistry, integrated DNA analysis devices, microelectrophoresis devices, microanalysis devices such as microchromatography devices, microdevices for preparing analytical samples such as mass spectra and liquid chromatography, extraction The present invention relates to a method for producing a microreactor useful as a physicochemical treatment device such as membrane separation and dialysis, and a spotter for producing a microarray.

Recently, there is a great need for technology to diagnose and analyze at low cost in the field, such as bedside diagnosis in the vicinity of patients, monitoring of environmental pollutants in the air, water and soil, and food safety inspection. It is getting higher.

For example, if an analysis that previously required an expensive and large device could be replaced by a small portable analyzer, a practitioner could install and use an analyzer that could only be installed in a large hospital. Thus, the diagnosis result can be easily and quickly fed back to the patient. In addition, the home health care environment can be improved by measuring the health index of the elderly by the family of the elderly and managing the health index numerical value at home or by regularly sending it to the hospital and managing it at the hospital. .

If environmental pollutants such as environmental hormones and dioxins can be easily measured without using an expensive and large apparatus, environmental diagnosis can be performed easily and inexpensively. Furthermore, if environmental pollutants can be analyzed on site using a small portable analyzer, a more detailed safe environment can be provided.

In order to perform such a measurement easily, it is necessary to be able to perform analytical detection with high sensitivity using a very small amount of sample. For this purpose, GC-MS, LC-MS, ICP-MS, which are separated by capillary gas chromatography (CGC), capillary liquid chromatography (CLC), induction plasma (ICP), etc., and detected by mass spectrometer (MS) Have been widely used as a method that can be analyzed with a small amount and high sensitivity.

However, since these devices are expensive and large-scale devices, it is very expensive to easily carry or install in a desired location, and can meet the needs of a wide range of medical diagnosis and environmental diagnosis as described above. There wasn't.

Therefore, recently, a method has been proposed in which a container for analysis is prepared by a micro processing technique or a nano processing technique, and this is used as a portable analyzer, that is, a microreactor.

Since this microreactor can be formed into a palm-sized size, it is easy to carry and can be suitably used for home medical diagnosis and mobile environment analysis. In addition, even when various kinds of complex reactions such as combinatorial chemistry must be performed at once, measurement can be performed with a very small amount of sample.

Furthermore, even in the case of chemical plants that perform liquid phase synthesis processes, there is a merit that heat transfer is very fast in the micro space even when heat exchange with high accuracy must be performed. It also has the potential to improve significantly.

In the microreactor, a fine channel for feeding a sample or the like, a storage tank for the sample, a reaction mechanism, a separation / extraction mechanism, a detection mechanism, or the like is formed according to the application.

For this reason, two substrates are overlapped to create a fine cavity having a predetermined shape, and necessary materials such as a liquid chromatograph gel, an electrode, and a micropump are placed in the predetermined cavity.

Various methods for creating a fine cavity of a predetermined shape by superimposing the two substrates are proposed. For example, between the member (A) and the member (B), by filling a solid substance excluding the portion that becomes the flow path, or on the surface on which the groove of the member (A) having a groove on the surface is formed By bonding the other member (B), the member (A) and the member (B) bonded to each other are viewed from the direction perpendicular to the bonding surface of the member (A) and the member (B). A method for producing a micro chemical device in which a capillary channel having a width of 1 to 1000 μm and a depth of 1 to 1000 μm is formed has been proposed.

As a method of providing grooves on the surface of the member (A), injection molding, solvent casting method, melt replica method, cutting, etching, photolithography, etching method, vapor deposition method, gas phase polymerization method,
There has been proposed a method of bonding a sheet-like member and a plate-like member obtained by cutting out a portion to be a groove (see, for example, Patent Documents 1, 2, and 3).
JP 2000-65791 A JP 2001-70784 A JP 2002-219697 A

Among the methods for creating a microcavity of a predetermined shape by superimposing the two substrates, the method of manufacturing a microreactor in the most inexpensive and large quantity is to create a mold having a microcavity of a predetermined shape, It is a method of transferring the unevenness of the mold to a synthetic resin plate or a method of creating a substrate by injection molding.

However, since the fine shape of the cavity desired for the microreactor is slightly different depending on the application, it is necessary to create a new mold for each application, and there is a disadvantage that the cost is high and time is required.

On the other hand, in order to accurately measure the substance to be detected and its concentration in the measurement sample, it is common to separate and extract only the detection substance from the measurement sample, and even when measuring with a microreactor. In general, a separation / extraction mechanism is formed in the microreactor.

Various separation / extraction mechanisms formed in the microreactor have been proposed, but the separation / extraction mechanism shown in FIG. 3 is the simplest and most useful separation / extraction mechanism.

That is, 13 and 23 in the figure have a cross-sectional width of 1-1000 μm and a cross-sectional height of 1-1000 μm.
m capillary microchannels, and the microchannels are open and in contact with each other on the C-plane. Since the microchannels 13 and 23 are very thin capillary microchannels as described above, the liquids D and E supplied to the microchannels 13 and 23 flow as a laminar flow, and C in which both are in contact with each other. On the surface, the liquid D flows through the fine flow path 13 without mixing the liquid D and the liquid E, and the liquid E flows through the fine flow path 2.
Go through 3.

Therefore, for example, when a measurement sample containing a substance to be detected is supplied as liquid D and a solvent capable of dissolving the substance to be detected is supplied as liquid E, the substance to be detected in the measurement sample is a solvent on the C plane. Extracted and separated.

In the case of the separation / extraction mechanism, if the liquids D and E supplied to the microchannels 13 and 23 do not flow in a laminar flow, the liquid D and the liquid E will be mixed and separation / extraction is impossible. End up. Therefore, it is not only essential that the microchannel 13 and the microchannel 23 are parallel to each other in the C plane, but also the F point where the microchannel 13 and the microchannel 23 merge, and the microchannel 13 and the microchannel. 2
The smaller the angle of the F point at which 3 is separated, the better. Specifically, it is preferably 20 degrees or less, more preferably 10 degrees or less.

Further, it is preferable that the tip of the point F is not rounded, that is, an acute angle is formed. If the tip of the point F is rounded, the liquid D or the liquid E follows the roundness, and even if the angle of the folding point F is reduced, the merging angle of both liquids is increased.

When creating a microreactor by a transfer method, an injection molding method, etc., it is necessary to create a mold having the same shape as this extraction / separation mechanism, but a fine channel having F and G points with very small angles. It was difficult to make the mold.

That is, the cheapest and simplest method for producing a mold is a method of cutting metal with a cutting tool. In this case, since the tool has a finite diameter, an acute angle of a part such as point F is used. It was difficult to get. Generally, a cutting tool for cutting has a diameter of about 50 μm, and it is impossible to obtain an acute angle such as a point F by this.

A mold can be made in advance by a method such as photolithography, and a metal can be transferred to the mold by electroforming. However, this method is relatively inferior to the above-mentioned method and is inferior in economic efficiency. When performing electroforming, defects such as the loss of sharp corners are likely to occur.

In view of the above-mentioned drawbacks, the object of the present invention is to extract two microchannels that intersect at a very small angle.
Providing a microreactor manufacturing method that can quickly create a mold with fine ridges that can form a separation mechanism at low cost, and that can quickly produce a microreactor with an extraction / separation mechanism at low cost. There is to do.

The method of manufacturing a microreactor having a separation / extraction mechanism according to the present invention uses a mold in which a plurality of divided molds are combined to form a recess corresponding to a fine cavity in a synthetic resin plate, which is laminated with a sheet member. A microreactor manufacturing method having a separation / extraction mechanism that forms a fine cavity between a synthetic resin plate and a sheet member, and in one split mold (A) of an adjacent split mold, Part of the fine channel forming ridge (a) is a part of the split mold (
The other split mold (B) of the adjacent split mold is formed at the end of A) so as to be parallel to the end face.
) And the fine channel forming ridges (b) are partly parallel to the end face at the end of the split mold (B), and the split mold (A) and split mold (B) ) Is assembled, the micro-channel forming ridges (
The part of a) and the part of the fine channel forming ridge (b) are formed adjacent to each other.

As the synthetic resin for forming the synthetic resin plate used in the present invention, any synthetic resin that has mechanical strength and can be formed into a plate shape can be used. For example, polystyrene, poly-α-methylstyrene Styrene polymers such as styrene-maleic acid copolymer and styrene-acrylonitrile copolymer; polysulfone polymers such as porsulfone and polyethersulfone; (meth) acrylic polymers such as polymethyl methacrylate and polyacrylonitrile; Polymaleimide polymers; Polycarbonate polymers such as bisphenol A polycarbonate, bisphenol F polycarbonate and bisphenol Z polycarbonate; Polyolefins such as polyethylene, polypropylene, poly-4-methylpentene-1, and ethylene-vinyl acetate copolymer Polymer Chlorine-containing polymers such as polyvinyl chloride, chlorinated polyvinyl chloride, and polyvinylidene chloride; cellulose polymers such as cellulose acetate and methyl cellulose; polyurethane polymers; polyamide polymers; polyimide polymers; Polyether-based or polythioether-based polymers such as 6-dimethylphenylene oxide and polyphenylene sulfide; polyetherketone-based polymers such as polyetheretherketone;
Examples thereof include polyester polymers such as polyethylene terephthalate and polyarylate; epoxy resins; urea resins; phenol resins.

In the present invention, a concave portion corresponding to a fine cavity is formed on the synthetic resin plate by a transfer method, an injection molding method, a powder press method or the like using a mold.

As the transfer method, any conventionally known transfer method may be employed.For example, when a thermoplastic resin is used as a synthetic resin, a plate-like body of the thermoplastic resin is prepared in advance, The method of heating the plate-like body of a thermoplastic resin and pressing both is mentioned.

In addition, when using a thermosetting resin or a photocurable resin as a synthetic resin, create an uncured or semi-cured plate, press the plate and the mold, and then heat or irradiate And a method of curing the thermosetting resin or the photocurable resin.

As the injection molding method, any conventionally known injection molding method may be employed.
For example, a method may be used in which a synthetic resin is supplied to an injection molding machine in which a mold is installed in the cavity.

When using a thermoplastic resin as a synthetic resin, it is only necessary to supply the molten thermoplastic resin to the cavity and take out the molded product after cooling, and when using a thermosetting resin as the synthetic resin, it is uncured. Alternatively, a semi-cured thermosetting resin may be supplied to the cavity, heated, cured, and then cooled to take out the molded body.

When using a photo-curing resin as a synthetic resin, supply an uncured or semi-cured photo-thermosetting resin to the cavity, heat it, take out the semi-cured molded product, and cure it by light irradiation. Just do it.

As the powder pressing method, any conventionally known powder pressing method may be adopted, and examples thereof include a method of forming a synthetic resin powder by supplying it to a mold and performing heat pressing.

In the present invention, a mold obtained by combining a plurality of divided molds is used as the mold. A microreactor is formed with a fine flow path for transferring a sample, a sample storage tank, a reaction mechanism, a separation / extraction mechanism, a detection mechanism, a waste liquid storage tank, etc. Since the size is different and the size is different, for example, a split mold for forming a fine flow path for feeding a storage tank, a reaction tank, a sample, etc., a fine flow for feeding a reaction mechanism, a sample, etc. Split mold for forming the channel, separation / extraction mechanism and fine channel for forming the fine channel for feeding the sample, etc., and fine channel for feeding the detection mechanism and the sample etc. are formed For this purpose, a split mold such as a split mold for forming a fine flow path for feeding a split mold, a waste liquid storage tank and a sample is prepared in advance, and these split molds are combined as necessary. A mold is created and a combined mold is used.

In the present invention, the mold for forming the separation / extraction mechanism is composed of two adjacent divided molds. One split mold (A) of the adjacent split mold has a fine channel forming projection (a) partly parallel to the end face at the end of the split mold (A). Is formed.

Also, the other split mold (B) of the opposed split molds has a fine channel forming projection (b),
A portion of the split mold (B) is parallel to the end face of the split mold (B), and when the split mold (A) and the split mold (B) are assembled, the fine channel forming projections ( It is formed so that the part of a) and the part of the fine channel forming projection (b) are adjacent to each other.

Therefore, when transferring to the synthetic resin plate by the transfer method, injection molding method, etc. using the above mold,
A separation / extraction mechanism as shown in FIG. 3 is formed on the synthetic resin plate.

That is, the fine flow path 13 formed by the fine flow path forming ridges (a) and the fine flow path 23 formed by the fine flow path forming ridges (b) have a fine flow path on the C plane. Open and in contact.

Therefore, for example, when a measurement sample containing a substance to be detected is supplied as liquid D and a solvent capable of dissolving the substance to be detected is supplied as liquid E, the substance to be detected in the measurement sample is a solvent on the C plane. Extracted and separated.

When a measurement sample containing a substance to be detected is supplied to the fine flow path 13 and an extract is supplied to the fine flow path 23, the extraction that has flowed through the fine flow path 13 from the measurement sample that has flowed through the fine flow path 13 In order to extract a substance to be detected in the liquid, both the measurement sample and the extraction liquid need to flow as a laminar flow, and since it is preferable that the contact area is wide, both the microchannels 13 and 23 are substantially square. It is preferable that the cross-sectional shapes of the fine channel forming ridges (a) and the fine channel forming ridges (b) are substantially square.

Further, since both the measurement sample and the extract flowing in the fine flow path 13 and the fine flow path 23 need to flow as a laminar flow, the fine flow path forming ridge (a) and the fine flow path forming ridge (b) The cross-sectional width of
It is preferably 3000 μm and the cross-sectional height is preferably 1 to 3000 μm, and more preferably both the cross-sectional width and the cross-sectional height are 1000 μm or less.

Even if the cross-sectional width and the cross-sectional height are larger than 3000 μm, a laminar flow can be formed by devising the liquid feeding speed or making the pulsation during liquid feeding as small as possible. A thinner one can more easily form a laminar flow.

Also, if the cross-sectional area of the flow path becomes too large, it will be necessary to take time until the molecular diffusion of the measured substance reaches the entire cross-sectional width of the flow path in order to fully extract the measured substance in the fluid during the extraction operation. Occurs.

For example, in the case of using a substance to be measured that diffuses a molecule of 25 μm per second, it takes 2 minutes to diffuse a cross-sectional width of 3000 μm. In general, if the analysis operation takes 2 minutes or more, it will not be suitable for the purpose of the above-mentioned simple analysis and on-site analysis, so the flow path cross-sectional width used here is preferably 3000 μm or less.

Furthermore, in the case of the separation / extraction mechanism, if the measurement sample and the extract supplied to the microchannels 13 and 23 do not flow in a laminar flow, the measurement sample and the extract are mixed and separation / extraction is not possible. It becomes impossible. Therefore, it is not only essential that the microchannel 13 and the microchannel 23 are parallel to each other in the C plane, but also the F point where the microchannel 13 and the microchannel 23 merge, and the microchannel 13 and the microchannel. The angle of the F point at which 23 is separated is preferably as small as possible, and when the divided mold (A) and the divided mold (B) are assembled, the fine flow path forming ridge (a) and the fine flow path forming It is preferable that the angle of both of the points where the ridge (b) contacts is 10 degrees or less.

In the present invention, since the concave portion corresponding to the fine cavity is formed in at least one synthetic resin plate by the transfer method, the injection molding method, or the like using the above mold, the divided mold is adjacent to the divided mold. It is necessary to fasten and fix the mold, and the split mold is preferably fixed to the adjacent split mold by a meshing mechanism.

The synthetic resin plate in which the concave portion corresponding to the fine cavity is formed is laminated with the sheet member,
A microreactor that forms a fine cavity between the synthetic resin plate and the sheet member is manufactured.

As the sheet member, any conventionally known sheet member such as a plate, sheet, or film manufactured from a synthetic resin, ceramics, metal, or the like can be used, but a material that can be firmly bonded to the synthetic resin plate is preferable. Therefore, a sheet member made of a synthetic resin constituting the synthetic resin plate is preferable.

In addition, as the above lamination method, any conventionally known production method may be adopted, for example, a method of bonding with a solvent-type adhesive or a pressure-sensitive adhesive, a method of bonding with a hot-melt type adhesive or a pressure-sensitive adhesive, or a solventless bonding. Examples thereof include a method of applying an energy ray curable resin as an agent, and curing and bonding by energy ray irradiation, an adhesion method by solvent application, a fusion adhesion method by heat or ultrasonic waves, and the like.

Next, the metal mold | die used by this invention is demonstrated with reference to drawings. FIG. 1 is a schematic plan view showing an example of a mold used in the present invention, and FIG. 2 is an enlarged view of a portion X in FIG.

The mold is formed by combining the divided mold A and the divided mold B. The split mold A is provided with convex portions for forming the microfluidic storage unit 1, the microdetection unit 3 and the waste liquid storage unit 4. The split mold B is formed with a convex portion for forming the minute extract storage part 2.

Further, the split mold A is connected to the convex portion for forming the fluid sample injection microchannel 11 connected to the microfluid storage section 1, the microfluid storage section convex section 1 and the waste liquid storage section convex section 4. A convex portion for forming the fluid sample fine flow path 12, and a convex portion for forming the extract fine flow path 22 that connects the micro extract storage section 2 and the micro detection section 3; A convex part for forming a waste liquid fine channel 31 for connecting the waste liquid storage unit 4 and a degassing fine channel 4 for connecting the waste liquid storage unit 4 and the outside.
Projections for forming 1 are formed.

The split mold B has a convex portion for forming an extract injection microchannel 21 connected to the micro extract storage unit 2 and an extract for connecting the micro extract storage unit 2 and the micro detection unit 3. Projections for forming the fine flow path 22 are formed.

As shown in FIG. 2, the projection for forming the fluid sample fine channel 12 is bent, and the split mold (A) has a part thereof parallel to the end face of the split mold (A). It is formed in the edge part. In addition, the ridges for forming the extraction liquid fine flow path 22 are also bent, and a part thereof is a split mold (B
) Is formed at the end of the split mold (B) so as to be parallel to the end surface.

And, a portion of the part formed at the end of the split mold (A), a ridge for forming the fluid sample fine channel 12, and a part of the part formed at the end of the split mold (B), The ridges for forming the extraction liquid fine channel 22 are formed adjacent to each other when the divided mold (A) and the divided mold (B) are assembled.

The cross-sectional shape of the ridges for forming each of the fine channels is a square having a width of 100 μm and a height of 100 μm, and the ridges and the extract for forming the fluid sample fine channel 12 at points F and G. The angle with the ridge for forming the fine channel 22 is 5 degrees.

The structure of the manufacturing method of the microreactor of the present invention is as described above, and a mold is formed by combining a plurality of divided molds, and a recess corresponding to a fine cavity is formed in a synthetic resin plate. Compared to creating a mold for each microreactor, it is only necessary to create a small divided mold, and it is possible to quickly create a divided mold having a fine cavity of a predetermined shape at a low cost. It can be quickly manufactured at low cost.

Further, one of the divided molds (A) of the adjacent divided molds has a fine channel forming projection (a) part of which is parallel to the end face at the end of the divided mold (A). In the other divided mold (B) of the adjacent divided molds, the fine flow path forming ridge (b) is partly connected to the end face of the end of the divided mold (B). When the split mold (A) and the split mold (B) are assembled in parallel, the part of the fine channel forming ridges (a) and the fine channel forming ridges (b) Therefore, a mold in which the fine channel forming ridges (a) and the fine channel forming ridges (b) intersect at a low angle is easily and inexpensively manufactured. be able to.

In addition, the extraction / separation mechanism formed by this mold can reduce the angle at which the fine flow path through which the measurement sample containing the substance to be detected flows and the fine flow path through which the extract flows intersect, The laminar flow of the extraction liquid is not disturbed, the extraction can be performed efficiently, and the detection accuracy of the microreactor is excellent.

Furthermore, the extraction / separation mechanism formed by this mold has an acute angle that is advantageous for laminar flow formation at the intersection of the fine flow path through which the measurement sample containing the substance to be detected flows and the fine flow path through which the extract flows. Therefore, the laminar flow of the measurement sample and the extraction liquid is not disturbed, and the extraction can be performed efficiently.

Formation of an acute angle has been impossible until now with cutting using a cutting tool, but by using a split mold as in the present invention, a mold for an extraction / separation mechanism can be produced at low cost. Is possible.

  Next, examples of the present invention will be described, but the present invention is not limited to the examples.

(Example 1)
The mold shown in FIGS. 1 and 2 is installed in a transfer mold for hot stamping, and a polymethyl methacrylate resin plate having a thickness of 5 mm is supplied between the receiving molds and preheated at 150 ° C. for 5 minutes. Then, a polymethylmethacrylate resin substrate having concave portions such as the fine flow path, the fine storage portion, and the detection portion shown in FIG. 1 was obtained by heating and pressing at 190 ° C. for 30 minutes. The convex part of the mold was accurately transferred to each concave part.

Next, an energy ray curable resin is applied to a polymethyl methacrylate resin plate having a thickness of 5 mm, and the obtained polymethyl methacrylate resin plate and the polymethyl methacrylate resin so that the energy ray curable resin layer and the concave portion are on the inside. The substrate was laminated, irradiated with light to cure the energy ray curable resin, and a microreactor in which both layers were adhered was obtained.

Next, one end of a silicon tube was connected to the microfluidic reservoir 1, a syringe loaded with a measurement solution was connected to the other end of the silicon tube, and the syringe was connected to a syringe pump. or,
One end of the silicon tube was also connected to the micro extract storage unit 2, a syringe for loading the extract was connected to the other end of the silicon tube, and the syringe was connected to a syringe pump.

Using each syringe pump, the microfluid storage unit 1 and the microextract storage solution for the measurement solution (aqueous solution in which 50 ppb of lead ions are dissolved in distilled water) and the extract (chloroform solution containing 0.2 mmol / l dithizone) The liquid was simultaneously fed to the fine channel 13 and the fine channel 23 through the part 2.

The liquid feeding speed at this time was 6 μl / min. The measurement solution flowing through the fine channel 13 and the extract flowing through the fine channel 23 formed a good laminar flow at the junction (from point F to point G).

After the microchannel 13 and the microchannel 23 are separated, the amount of lead in the extract is measured by an absorptiometric measurement method in the microdetection unit 3 of the extract flowing in the microchannel 23, and lead ions in the measurement solution Converted to concentration. The spectrophotometer is manufactured by Ocean Optics, product name “USB2”.
000 "was used. The obtained lead ion concentration was 51 ppb.

(Example 2)
A microreactor was obtained in the same manner as in Example 1 except that the microreactor was formed by injection molding instead of the hot stamping method. In the injection molding, the mold shown in FIG. 1 and FIG. 2 is installed in a mold for injection molding, and a polymethylmethacrylate resin plate is supplied between the receiving molds as shown in FIG. A polymethylmethacrylate resin substrate having concave portions such as a fine flow path, a fine storage portion, and a detection portion was obtained. The molding temperature was 210 ° C., and the convex portion of the mold was accurately transferred to each concave portion.

Next, when the extraction process was performed in the same manner as in Example 1 using the obtained microreactor, a good laminar flow was formed at the merged portion, and the lead ion concentration in the obtained measurement solution was 53 ppb.

(Example 3)
Example 1 except that the angle between the ridges for forming the fluid sample microchannels 13 at the points F and G and the ridges for forming the extract microchannels 23 was 15 degrees. A microreactor was obtained in the same manner as performed.

Next, when the extraction process was performed in the same manner as in Example 1 using the obtained microreactor, a good laminar flow was formed at the merged portion, and the lead ion concentration in the obtained measurement solution was , 51 ppb.

(Comparative Example 1)
A microreactor was obtained in the same manner as in Example 1 except that an integral transfer mold for hot stamping having irregularities shown in FIGS. 1 and 2 was used, and then an extraction operation was performed.
The F and G points in the microreactor were rounded.

Next, when the extraction process was performed in the same manner as in Example 1 using the obtained microreactor, a phenomenon in which the interface between the measurement solution and the extract was undulated was observed at the merged portion, and also at the separation portion. Each liquid was not separated well.

It is explanatory drawing which shows an example of a separation / extraction mechanism. It is a plane schematic diagram which shows an example of the metal mold | die of a microreactor. FIG. 3 is an enlarged view of a portion X in FIG. 2.

Explanation of symbols

A, B Divided mold 1 Micro fluid storage unit 2 Micro extract storage unit 3 Micro detection unit 4 Waste liquid storage unit 11, 12, 13, 21, 22, 23, 31, 41 Micro flow path

Claims (5)

Using a mold in which a plurality of divided molds are combined, a concave portion corresponding to the fine cavity is formed in the synthetic resin plate, and this is laminated with the sheet member, thereby forming a fine cavity between the synthetic resin plate and the sheet member. A method of manufacturing a microreactor having a separation / extraction mechanism to be formed, in which one split mold (A) of adjacent split molds is provided with a fine channel forming projection (a), a part of which Formed at the end of the split mold (A) so as to be parallel to the end face, the other split mold (B) of the adjacent split mold has a fine channel forming convex strip (b) A part of the split mold (B) is parallel to the end face, and when the split mold (A) and the split mold (B) are assembled, the fine channel forming protrusion (a ) And the part of the fine channel forming ridge (b) are adjacent to each other. Method for manufacturing a microreactor having a separation and extraction mechanism. 2. The method of manufacturing a microreactor having a separation / extraction mechanism according to claim 1, wherein the split mold is fixed to an adjacent split mold by a meshing mechanism. The microreactor having a separation / extraction mechanism according to claim 1 or 2, wherein the cross-sectional shapes of the microchannel-forming ridges (a) and the microchannel-forming ridges (b) are substantially square. Production method. The cross-sectional widths of the fine channel forming ridges (a) and the fine channel forming ridges (b) are 1 to 3000 μm.
The method for producing a microreactor having a separation / extraction mechanism according to claim 3, wherein the cross-sectional height is 1 to 3000 μm. When assembling the divided mold (A) and the divided mold (B), the angle between the contact points of the fine channel forming ridges (a) and the fine channel forming ridges (b) is 20 degrees. The method for producing a microreactor having a separation / extraction mechanism according to any one of claims 1 to 4, wherein:

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