JP2004504003A - 多能性胚様幹細胞、その組成物、方法および使用 - Google Patents

多能性胚様幹細胞、その組成物、方法および使用 Download PDF

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JP2004504003A
JP2004504003A JP2001525326A JP2001525326A JP2004504003A JP 2004504003 A JP2004504003 A JP 2004504003A JP 2001525326 A JP2001525326 A JP 2001525326A JP 2001525326 A JP2001525326 A JP 2001525326A JP 2004504003 A JP2004504003 A JP 2004504003A
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stem cells
methods
present
pluripotent
cells
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JP2004504003A5 (ja
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ヘンリー　イー　ヤング
ポール　エイ　ルーカス
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Morphogen Pharmaceuticals Inc
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Morphogen Pharmaceuticals Inc
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Priority claimed from PCT/US2000/026225 external-priority patent/WO2001021767A2/en
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Publication of JP2004504003A5 publication Critical patent/JP2004504003A5/ja
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources

Abstract

本発明は、多能性幹細胞、特に、多能性胚様幹細胞に関する。本発明はさらに、多様性胚様幹細胞を精製する方法、ならびにその組成物、培養物およびクローンに関する。本発明はまた、本発明の多様性幹細胞をヒトなどの哺乳動物宿主に移植する方法であって、前記宿主に前記幹細胞を導入することを含む上記方法に関する。本発明はさらに、目的のタンパク質または遺伝子のｉｎ　ｖｉｖｏ投与方法であって、多能性幹細胞に目的のタンパク質をコードするＤＮＡを含む構築物をトランスフェクトし、次いで、宿主に前記幹細胞を導入し、ここで、目的のタンパク質または遺伝子が発現されることを含む上記方法に関する。本発明はまた、本発明の多能性幹細胞を培養または移植することによって、中胚葉、内胚葉または外胚葉系統前駆細胞を産生する方法に関する。

Description

【０００１】
【発明の分野】
本発明は、一般に多能性幹細胞、詳細には胚様多能性幹細胞に関する。本発明はまた、細胞または組織移植、遺伝子治療、ならびに細胞−細胞間相互作用、分化決定、発生遺伝子および成長もしくは分化因子に関して同定、アッセイまたはスクリーニングにおける組織工学のための幹細胞の使用に関する。
【０００２】
【発明の背景】
組織および器官の形成は、出生前の発生中に自然に生じる。多細胞生物の発生は、規定された機能を有する１０億個の細胞から成る実体の形成時に最大に達する予め決定された分子および細胞経路に続いて起こる。細胞の発生は、細胞の増殖、分化決定、および分化の進行（ｌｉｎｅａｇｅ−ｐｒｏｇｒｅｓｓｉｏｎ）によって達成され、その結果、分化した細胞型が形成される。この過程は全能性接合体から始まり、個体の生涯を通して継続する。発生が全能性接合体から進行しながら、細胞が増殖し、全能性初代胚葉、外胚葉、中胚葉、および内胚葉への分化決定によって分離する。先祖（多能性、三能性、両能性および終局的に単能性）系統への進行性分化決定によってこれらの胚葉がさらに分離することによって、細胞の分化経路および細胞の究極的機能がさらに規定される。

Claims (2)

  1. 非胚もしくは生後動物細胞または組織由来、自己再生可能ならびに内胚葉、外胚葉および中胚葉系統の細胞に分化可能な多能性胚様幹細胞。
  2. ヒト細胞である、請求項１に記載の幹細胞。
JP2001525326A 1999-09-24 2000-09-25 多能性胚様幹細胞、その組成物、方法および使用 Pending JP2004504003A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40489599A 1999-09-24 1999-09-24
US66850800A 2000-09-22 2000-09-22
PCT/US2000/026225 WO2001021767A2 (en) 1999-09-24 2000-09-25 Pluripotent embryonic-like stem cells, compositions, methods and uses thereof

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JP2004504003A5 JP2004504003A5 (ja) 2005-01-20

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JP2001525326A Pending JP2004504003A (ja) 1999-09-24 2000-09-25 多能性胚様幹細胞、その組成物、方法および使用
JP2010272045A Withdrawn JP2011103885A (ja) 1999-09-24 2010-12-06 多能性胚様幹細胞、その組成物、方法および使用
JP2011084816A Pending JP2011167198A (ja) 1999-09-24 2011-04-06 多能性胚様幹細胞、その組成物、方法および使用
JP2013232006A Pending JP2014054258A (ja) 1999-09-24 2013-11-08 多能性胚様幹細胞、その組成物、方法および使用
JP2015225380A Expired - Lifetime JP6539188B2 (ja) 1999-09-24 2015-11-18 多能性胚様幹細胞、その組成物、方法および使用
JP2017016498A Pending JP2017079792A (ja) 1999-09-24 2017-02-01 多能性胚様幹細胞、その組成物、方法および使用
JP2018159129A Expired - Lifetime JP6545335B2 (ja) 1999-09-24 2018-08-28 多能性胚様幹細胞、その組成物、方法および使用
JP2018225193A Pending JP2019076094A (ja) 1999-09-24 2018-11-30 多能性胚様幹細胞、その組成物、方法および使用

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JP2011084816A Pending JP2011167198A (ja) 1999-09-24 2011-04-06 多能性胚様幹細胞、その組成物、方法および使用
JP2013232006A Pending JP2014054258A (ja) 1999-09-24 2013-11-08 多能性胚様幹細胞、その組成物、方法および使用
JP2015225380A Expired - Lifetime JP6539188B2 (ja) 1999-09-24 2015-11-18 多能性胚様幹細胞、その組成物、方法および使用
JP2017016498A Pending JP2017079792A (ja) 1999-09-24 2017-02-01 多能性胚様幹細胞、その組成物、方法および使用
JP2018159129A Expired - Lifetime JP6545335B2 (ja) 1999-09-24 2018-08-28 多能性胚様幹細胞、その組成物、方法および使用
JP2018225193A Pending JP2019076094A (ja) 1999-09-24 2018-11-30 多能性胚様幹細胞、その組成物、方法および使用

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US (1) US20100239542A1 (ja)
EP (2) EP2333049A1 (ja)
JP (8) JP2004504003A (ja)
AT (1) ATE426016T1 (ja)
DE (1) DE60041821D1 (ja)
DK (1) DK1218489T3 (ja)
NZ (1) NZ518601A (ja)

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DK1218489T3 (da) 2009-06-02
JP2018174948A (ja) 2018-11-15
US20100239542A1 (en) 2010-09-23
NZ518601A (en) 2004-10-29
EP1903107A1 (en) 2008-03-26
JP2014054258A (ja) 2014-03-27
ATE426016T1 (de) 2009-04-15
JP2011103885A (ja) 2011-06-02
JP2011167198A (ja) 2011-09-01
DE60041821D1 (de) 2009-04-30
JP2016063818A (ja) 2016-04-28
JP6545335B2 (ja) 2019-07-17
JP2017079792A (ja) 2017-05-18
JP6539188B2 (ja) 2019-07-03
JP2019076094A (ja) 2019-05-23
EP2333049A1 (en) 2011-06-15

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