JP2003307521A - Water quality analyzing microreactor and water quality analyzer - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a microreactor and a water quality analyzer for easily and quickly analyzing a water quality. <P>SOLUTION: A spectral analysis is applied by constituting the microreactor where a supply part 4, a supply part 6, a supply part 8, and a supply part 10 are arranged on a base board 2, these supply parts are connected by a passage 12, and are mixed in prescribed order in a mixing section 16, and are colored in a coloring section 13, and water is made to flow to a measuring cell part 14. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a water quality analysis microreactor and a water quality analysis device for analyzing water quality simply and quickly.

[0002]

2. Description of the Related Art As a means for analyzing water quality, JIS (Japanese Industrial Standard) has been established for each substance to be analyzed, and these analysis methods are usually used.

For example, when the substance to be analyzed is hexavalent chromium, JIS K 0102 65.2. Explaining that the target sample is colorless and transparent,
(1) Sample 1 in a 50 ml graduated cylinder with stopper
Aliquot 10 ml. (2) 1 concentrated sulfuric acid with water
Add 2.5 ml of 0-fold diluted solution.
(3) Further, 1 ml of diphenylcarbazide solution is added. (4) Dilute to 50 ml with pure water. (5) Stir evenly and leave at about 15 degrees Celsius for 5 minutes. (6) The procedure of measuring the absorbance at a wavelength of 540 nanometers.

When the substance to be analyzed is chlorine, first, the following extraction operation is performed. That is, (1) 20
Take 0.2 to 0.5 grams of sample in a 0 milliliter beaker. (2) Dilute to about 100 ml with pure water. (3) Boil for 3 to 5 hours to evaporate a part of the water content and extract the chlorine content contained in the sample.
(4) Cool. (5) Filter with a filter paper of 1 micrometer. The pore size of the filter paper is not specified here, but generally No. 5C (1 μm) is used, and if the sample particle size is smaller, the membrane filter paper
(0.8 μm, 0.45 μm) etc. may be used. On the contrary, when the sample particle size is coarser, coarse filter paper may be used. (6) Dilute to 100 ml with pure water. Next, colorimetric analysis specified in JIS K 0101 32. 1 is performed. That is, (7) 1 to 10 ml of the sample is dispensed into a 50 ml graduated cylinder with a stopper.
(8) Add 10 ml of ammonium iron sulfate solution. (9) Further, 5 ml of mercury thiocyanate ethanol solution is added. (10) Dilute to 50 ml with pure water. (11) Stir evenly and make 1 at 20 degrees Celsius.
Leave for 0 minutes. (12) Measuring the absorbance with a spectrophotometer at a wavelength of 460 nanometers.

However, in this method, the steps up to the measurement of the absorbance are manual and the required time is long, which may cause a problem in that much labor is required to obtain the analysis result. When the inventor actually conducted a chlorine water quality analysis,
It is usual that the extraction operation from (1) to (6) requires 3 to 5 hours, the color development (reagent mixing) operation takes 15 minutes, the color development takes 10 minutes, and the absorbance measurement takes 20 minutes.
In addition to this, the time required for cleaning the beaker and the absorbance measuring cell is generated. Of course, an additional time is required to raise the test solution from room temperature to a predetermined extraction temperature.
Therefore, it takes 6 to 7 hours in total for one analysis.

[0006]

SUMMARY OF THE INVENTION The present invention has been made in view of the above problems of the prior art, and an object of the present invention is to provide a microreactor for performing water quality analysis simply and quickly. Another object of the present invention is to provide a simple and quick water quality analyzer that combines a microreactor and a spectroscopic analyzer.

[0007]

According to the present invention, a supply unit for supplying an analyte or a reagent, a flow path for mixing the analyte and the reagent, and a measurement cell unit are provided on a single substrate. It is characterized by being incorporated. According to the present invention, the operation can be speeded up by eliminating the work of transferring the analyte or the reagent. In addition, the width of the flow path is 50 micrometers or more 5
00 μm or less, preferably 100 μm or more and 200 μm or less, more preferably 150 μm or more 20
With a microchannel structure of 0 μm or less, the mixing speed of the analyte and the reagent can be dramatically increased.

A first embodiment according to the present invention is a substrate, one or more supply units provided on the substrate, a measurement cell unit provided on the substrate, the supply unit and the measurement cell unit. A microreactor for water quality analysis, which comprises a flow path connected to each other.

Here, the number, dose, arrangement order, mixing time, etc. of the above-mentioned supply parts are variously determined by each analytical method. Usually, one series to be described later has one sample supply section for storing a measurement target sample and one or more reagent supply sections for storing analysis reagents. The reagent supply unit also includes a solvent supply unit that stores the solvent of the sample.

According to this embodiment, since the portion for supplying the sample to be analyzed or the reagent and the measuring cell are formed on one substrate, it is not necessary to transfer the contents. Further, since the sample to be analyzed and the reagent are mixed by merging the flow paths, it is not necessary to mix the contents.

In a second embodiment according to the present invention, the flow path has a width of usually 150 micrometers or more and 500 micrometers or less, preferably 150 micrometers or more and 200 micrometers or less, for water quality analysis. It is a microreactor. According to this embodiment, the flow channel exerts the microchannel effect, so that the mixing speed can be dramatically increased.

A third embodiment according to the present invention is characterized in that it includes two or more series including one or more of each of the supply section, the measurement cell section and the flow path. It is a micro reactor for analysis.

According to this embodiment, there can be a plurality of analysis series. For example, when the number of series is 2, the analysis of the sample to be analyzed and the blank measurement can be simultaneously performed on one substrate. When the number of series is 3, the analysis of the sample to be analyzed, the blank measurement, and the analysis of the reference solution can be simultaneously performed on one substrate.

Here, one series is composed of at least one measurement cell section, a flow path whose one end is connected to the measurement cell section, and one or more supply sections connected to the other end of the flow path. To be done. It does not matter whether or not the flow path is branched. Various reagents are usually pressure-fed to the supply unit by an external pump and supplied to the measurement cell unit. As a method for moving the sample, for example, when pumping the sample,
It can be sent at about 60 μl / min. A liquid reservoir may be provided in the middle of the supply unit.

A fourth embodiment according to the present invention is a microreactor for water quality analysis characterized by comprising one or more solid-liquid separation means in the middle of the flow path. here,
Examples of solid-liquid separation means include membrane filter paper and GFP
(Glass fiber paper) or the like is used. According to this embodiment, even when a small amount of solid content is mixed in the content, it is possible to prevent clogging of the flow path and reliably perform water quality analysis.

A fifth embodiment according to the present invention is a microreactor for water quality analysis, characterized by comprising one or more heating means in the middle of the flow path. Here, as the heating means, for example, a copper wire, a nichrome wire, a ceramic heater, or the like, which uses light (laser, halogen, xenon, etc.) can be considered. While copper wire or nichrome wire is cheaper, it is easier to control using light in terms of temperature and time until temperature rise. On the other hand, the method using light has an advantage that the temperature is raised quickly, but it is expensive because a light source is separately required. It is also necessary that the flow path embedded in the microreactor substrate is a material that absorbs the light. It is possible to efficiently irradiate and heat the pinpoint of the flow path in the microreactor with a means such as an optical fiber. The above heating means,
For example, it is preferable that the heating element (linear) is arranged along the flow path or the flow path is covered with the heating element. As the power source, it is preferable to use an external power source or a battery embedded in a microreactor.

Further, it is possible to provide two or more heating means depending on the sample and purpose. For example, the first heating means heats the solvent and the second heating means heats the liquid containing the sample. Things to do are possible. According to this embodiment, water quality analysis can be performed even when a heating operation is required as a pretreatment when the substance to be analyzed is chlorine, for example.

A sixth embodiment of the present invention is a microreactor for water quality analysis characterized by using a shaker for a part of the heating means, and a shaker is provided for improving mixing and stirring efficiency. Be attached. Heat can be expected due to the secondary effect of shaking by a shaker. An oscillating device is attached to the shaker, and examples of the oscillating device include a crystal oscillator, a piezo element, and a laser oscillator. Among them, the piezo element can be preferably used in the present invention. At the time of shaking, in addition to ultrasonic shaking, a circular weight may be installed around the shaft, and the center position may be shifted to rotate at high speed to generate vibration. Alternatively, the container may be filled with a fluid, an object may be sealed in the container, and the object may be moved to generate the vibration. Since these have a relatively simple structure, they can be manufactured inexpensively and easily.

When the microreactor is shaken, the vibration may spread to a portion (corresponding to the heater position) where stirring by shaking is necessary. Therefore, it is desirable that the detection operation is not performed during the time when the vibration is generated because the vibration is adversely affected when the vibration is detected. Since the piezo element is small and various forms can be selected, it is possible to give vibration of a form and intensity required on the microreactor. When giving vibration for the purpose of mixing and stirring, if the vibration is too weak, the effect does not appear, and if the vibration is too strong, cavitation phenomenon may occur and bubbles may be generated in the flow path. It is necessary to adjust the shaking effect to the appropriate vibration.

According to this embodiment, a shaker can be used to dissolve or finely disperse particles of a hardly soluble sample dispersed in a solvent by breaking the particles. Further, it is possible to expect high efficiency of extraction, and it is possible to further shorten the analysis time.

In a seventh embodiment according to the present invention, one of the supply units is arranged inside the substrate, and the rest of the supply unit and the measurement cell unit are arranged on the surface of the substrate. It is a microreactor for water quality analysis characterized by the following.

According to this embodiment, since the inside of the substrate can be used for heating and the surface of the substrate can be used for cooling, the temperature raising or lowering operation can be efficiently performed in a short time. be able to. That is, the solid sample can be stored in the supply unit arranged inside the substrate, and the extraction and the dissolution in the solvent can be performed inside the substrate by heating, and the substrate surface can be efficiently cooled. it can.

An eighth embodiment according to the present invention is a water quality analysis microreactor characterized in that the supply part and the measurement cell part are arranged open to the outside of the substrate.

According to this embodiment, the sample to be analyzed can be continuously supplied from the outside, and the test liquid after the measurement can be continuously discharged from the measurement cell section to the outside by overflowing. It can be applied to both analysis and continuous analysis.

A ninth embodiment according to the present invention is the microreactor for water quality analysis according to any one of the above, wherein the flow path is connected to the lowermost part of the measurement cell section. In this embodiment, the solution flows in from the lower part of the measuring cell and the overflow is discharged from the upper part, which facilitates replacement of the liquid flowing into the measuring cell part. Further, with respect to the flow path flowing into the measurement cell section (detection section), for example, a mode in which the flow path of the solution from the supply section is inclined toward the lowermost part of the measurement cell section is preferable.

The tenth embodiment according to the present invention is a water quality analyzer comprising the water quality analysis microreactor according to any one of the above and a spectroscopic analyzer. According to this embodiment, the pretreatment and measurement can be performed in a state where the microreactor is loaded in the spectroscopic analyzer, so that the operation is further simplified.

The eleventh embodiment of the present invention is a water quality analyzer further including data output means connected to the spectroscopic analyzer. According to this embodiment, the analysis data output from the data output means can be stored in the computer at the site directly connected by wire, so that it can be publicly used for data processing. Further, according to this embodiment, since the data can be stored in the computer at the remote place connected through the communication line from the data output means, the data output from the water quality analyzer installed at the measurement site can be used at any point. be able to.

[0028]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the microreactor for water quality analysis of the present invention will be described below in detail with reference to the drawings.

FIG. 1 is a perspective view showing the microreactor of the present invention. Here, the supply portions 4, 6, 8 and 10 having a shape capable of storing the solution on the substrate 2, for example, a circular supply portion are used.
Is provided. The supply unit 4 is a sample supply unit, and the supply units 6, 8 and 10 are reagent supply units, of which the supply unit 1
Reference numeral 0 is a solvent supply unit. A pump (not shown) is connected to each supply unit so that the fluid flows from left to right as viewed in the drawing. In the drawing, the left side is the pre-processing section 16 and the right side is the coloring section 18. This micro reactor is JIS
When used in accordance with K 0102 65.2, the sample supply unit 4 is a sample to be measured, the supply unit 6 is a 10-fold diluted concentrated sulfuric acid with pure water, and the supply unit 8 is a diphenylcarbazide solution. Pure water may be supplied to the solvent supply unit 10, respectively. By supplying in this way, JIS K
The sample and reagents will be mixed in the order defined in 65.2. The color-developing portion 18 is for performing a procedure corresponding to leaving for 5 minutes specified by JIS. Here, a pump can be cited as an effective means for flowing the fluid, and the fluid can be pumped by the pump, but gravity or centrifugal force can be used instead of the pump.

The material of the substrate 2 is not particularly limited, but in the present invention, it is preferable to use a transparent material because it is preferably used for spectroscopic analysis. Specifically, quartz, glass, various transparent polymers and the like can be used.

Since FIG. 2A is a plan view of FIG. 1, a description thereof will be omitted. 2 (b) is shown in FIG.
It is a figure which shows the II cross section of (a). Here, the flow path 12
Is disposed inside the substrate 2, and the cross section thereof may be a shape having corners as shown in the drawing, or may be circular or corrugated. Therefore, there is no possibility that the fluid overflows from the flow path 12. FIG. 2C is a view showing a II-II cross section of FIG. Here, since the measurement cell unit 14 is deeper than the flow channel 12, it is possible to earn the absorbance. Further, in the apparatus of FIG. 2, since the upper surface of the measurement cell unit 14 is open, it is suitable for discarding the test solution after the absorbance measurement, but the upper surface of the measurement cell unit is not necessarily open. good.

Examples of the material of the measuring cell portion include polystyrene, glass, quartz and the like, and glass is particularly preferable in a microreactor accompanied by heating. The volume of the measurement cell unit is preferably about 200 μl,
With 200 μl, a correlation coefficient of R ² = 0.97 or more can be obtained. Also, in the optical system in the measurement cell part, the light is normally irradiated perpendicularly to the cell, and the wavelength is 5 in the case of hexavalent chromium.
40 nm, and in the case of chlorine, 460 nm.

In order to manufacture the microreactor having such a structure, the substrate 2 may be composed of two parts. That is, the supply parts 4, 6, 8 and 10 and the flow path 12
A first member engraved with the measurement cell unit 14 and a second member with a round hole corresponding to the measurement cell unit 14 prepared,
The microreactor of the present invention can be produced by bonding the first member and the second member together.

The shape and size of the microreactor are not particularly limited, but it is preferable to design the microreactor according to the shape and size of the measurement cell storage portion of the spectroscope. Although the specific dimensions of the microreactor vary depending on the manufacturer and type of the spectroscope, the shape is usually a thin rectangular parallelepiped shape as shown in FIG. 1, with a length of 10 to 100 mm, a width of 10 to 50 mm, and a thickness of 5 mm. It is generally about 10 to 10 mm.

FIG. 3 is a development of the embodiment shown in FIG.
Two microfluidic channels are provided in one microreactor. Thereby, for example, the analysis target sample can be measured in one series, and the blank can be measured in the other series. In this case, the spectroscopic analyzer will select one of the positions of the measurement cell unit 14 or 14 'and measure each one. To select a position, it is convenient to use a spectroscopic analyzer having a coordinate control function.

FIG. 4 shows a further development of the embodiment shown in FIG. 3 in which three channels are provided in one microreactor. As a result, the first series measures the sample to be analyzed, the second series measures the blank,
The third series can measure standard samples. As a standard sample, for example, when a solution having a concentration of an environmental standard value defined by relevant laws and regulations is used, it is possible to clearly compare the concentration relationship between the sample to be analyzed and the environmental standard value.

The number of such series can be further increased, as the dimensions of the spectrophotometer allow. If a microreactor having a large number of series is used, there is an advantage that a calibration curve can be easily prepared by passing a standard solution having different concentrations in each series.

FIG. 5 shows an example of a microreactor that can be suitably used for continuous analysis. Here, the supply units 34, 3
Nos. 6, 38 and 40 do not have a liquid reservoir, and are composed only of flow paths connected to an external pump (not shown). Also,
A discharge channel 42 is connected to the measurement cell unit 14 so that the used test liquid can be discharged to the outside. In this case, it is desirable that a waste liquid reservoir (not shown) is provided in front of the discharge flow path 42 to prevent the inside of the spectroscopic analyzer from being contaminated.

FIG. 6 shows an example of a microreactor that can be suitably used for chlorine analysis. The microreactor having the shape and dimensions shown in FIG. 6 can be particularly suitably used when the measurement target sample is 200 microliters. This will be described later together with the measurement data.

FIG. 6A is a plan view and FIG. 6B is III.
It is a figure which shows a III 'cross section. Here, pure water is supplied from the supply unit 4 using a pump (not shown). Figure 6
The part shown by the dotted line in (a) shows the section in which the flow path is three-dimensionally depressed. As shown in FIG. 6B for details, the supply unit 6 is provided inside the microreactor (near the back surface in the drawing).
Is provided. By forming the flow channel into a three-dimensional structure, it is possible to heat only the recessed portion of the flow channel, and it is also possible to easily cool the heated liquid.

The supply unit 6 contains about 2 milligrams of the sample to be measured. Normally used chemical balance is 0.1
Since the measurement is performed in milligram units, if the measurement target sample is 2 milligrams, the weighing error can be reduced to 1/20. Since the measurement accuracy of the present invention is governed by R ² which is the correlation coefficient of the calibration curve described later, if the weighing error is 1/20, the accuracy can be balanced.

Water on the way from the supply unit 4 to the supply unit 6 is heated to 10 degrees Celsius by the heater 22 which is the first heating means.
It is heated to about 0 degrees. The heated water passes through the supply unit 6 to come into contact with the sample contained therein and is extracted as a solvent. Water on the way from the supply section 6 to the surface of the microreactor is heated by the heater 24, which is the second heating means.
Is heated to about 200 degrees Celsius. This does not need to be strictly 200 degrees Celsius, and there is no problem even within an error range of about 40 degrees up and down. Chlorine contained in the sample to be measured is extracted by the heat generated by these heaters. The heating part is installed in the recessed part of the flow path.
As a result, the heating-free parts at the front and rear of the flow path are
It can be arranged so that heat cannot be transmitted easily. In addition, the three-dimensional structure of the flow path also has the effect of eliminating the need for installing heat insulating means between the heating portion and the cooling portion.

The mesh 30 provided in the middle of the flow channel has a function of preventing clogging of the flow channel and further preventing the sample from flowing out from the supply section 6. After the predetermined heating is completed, the fluid is cooled by the cooling section 26. Since a part of the cooling section 26 is near the surface of the microreactor, the cooling effect is high.

A filter paper 28, which is a solid-liquid separating means, is installed at the end of the cooling section 26, and even if the solid content is deposited by cooling, it is captured. If the solid content is mixed, not only the flow path is clogged, but also the spectroscopic analysis is hindered, which is not preferable. The type of the filter paper 28 that is a solid-liquid separation means is not particularly limited, and for example, glass fiber paper (GFP) is used.
Polycarbonate paper (PC) can be used. Further, a filter made of another material may be used instead of the filter paper.

From the supply unit 8, an ammonium sulfate iron sulfate solution is supplied by using a pump not shown, and from the supply unit 10, a mercury thiocyanate ethanol solution is supplied by using a pump not shown.
Supply each. As a result, the reagents are mixed in the mixing section 16 of the flow channel 12 in accordance with the method defined in JIS K 010132.1 for the entire microreactor. The color development section 18 is on the right side of the flow path 12.

A measuring cell portion 14 is arranged in front of the flow path 12. The measuring cell unit 14 here is designed to have a capacity of 200 microliters, and specifically, for example, a cylindrical shape having a diameter of 8 mm and a depth of 5 mm can be mentioned. This cylinder has 251
Although there is a volume of microliters, from the viewpoint of preventing overflow during measurement, 200 microliters at about the eighth minute is put in and used. The content is 140 microliters of pure water supplied from the supply unit 4, 40 microliters of ammonium iron sulfate solution supplied from the supply unit 8 and 20 microliters of mercury thiocyanate ethanol solution supplied from the supply unit 10.

On the other hand, the longer the optical path length, the stronger the absorbance that can be obtained.
It is possible to increase the optical path length by narrowing the diameter (area) of the cell portion and keeping it longer in the depth direction while maintaining the above condition. The shape of the measurement cell portion may be a polygonal columnar shape such as a quadrangular prism or the like in addition to a cylindrical shape. Specifically, for example, it may be a measuring cell portion (calculated volume of 250 μl) having a depth of 10 mm for an area of 5 mm square. By taking a long optical path length in this way, there is an effect that a strong absorbance can be obtained. The optical path length (measurement cell portion depth) cannot be longer than the thickness of the microreactor, and the area of the measurement cell portion is restricted by the diameter of the spectroscopic light source used for measurement.

The material of the microreactor is made of glass or quartz, and is designed to withstand heating by the heater 24 at about 200 degrees Celsius. In addition, when the diameter of the flow channel is 500 μm or less in the case of a circular shape and 500 μm or less per side in the case of a rectangular shape, the microchannel effect starts to appear. Particularly preferred conditions are diameter 20
It is a 0 μm cylindrical cross section flow path or a 200 μm side rectangular cross section flow path.

Here, the total amount of fluid passing through the flow path 12 is 20.
Since it is 0 microliters, by flowing this section at 60 microliters per minute, the time required for passage becomes 3 minutes and 20 seconds. Such a minute flow rate pump is also widely used by those skilled in the art.

By the way, under the conditions of this embodiment, the color development time from the addition of the last ethanol solution of mercury thiocyanate as the color development reagent to the measurement cell portion 14 is 3 minutes and 20 seconds. According to the JIS K 0101 32.1 standard, the coloring time is set to 5 minutes, so that the coloring time is shorter than the standard in this embodiment. However, it is speculated that the color development proceeds sufficiently due to the microchannel effect.

Here, the microchannel effect means an effect of efficiently admixing due to molecular diffusion when a fluid passes through a minute channel, and is widely known to those skilled in the art. As an example, it is known that the mixing operation which conventionally takes 10 hours can be completed in 10 minutes by utilizing the microchannel effect. In addition, it is known that the molecular diffusion time of the latter is 1 / 10,000 when comparing the flow channel width of 1 cm and 100 μm (“Chemical process microchip integration ", Professor Takehiko Kitamori of the University of Tokyo, lecture on May 30, 2001).

Here, it is certain that there is concern that the absorbance may be attenuated due to the excessive progress of the reaction due to the microchannel effect. In fact, J
5 using a 10 mm cell as defined by IS
Data is obtained that the absorbance when left for 60 minutes is about half of that when left alone for 60 minutes. That is, an incorrect analysis value will be obtained unless the leaving time is accurately determined. However, this problem can be solved by using a spectroscopic analyzer having a peak measurement function, and is not affected in practice.

Further, according to the present embodiment, there is an advantage that the amount of the sample to be analyzed and the coloring reagent are small. That is, JI
In SK 0101 32.1, it is specified that a test solution of 4 milliliters is used using a cell of 10 millimeters, but the present invention requires 200 microliters. Such downsizing can reduce the amount of color-developing reagent used.

Up to this point, only the case where the substance to be measured is hexavalent chromium or chlorine has been described, but the embodiment of the present invention is not limited to these. Specifically, in the microreactor of the present invention, the substance to be measured is phosphoric acid (JIS K 0
102 46.1) and silica (JISK 0101 44.1) can be similarly applied.

The substance to be analyzed is fluorine (JIS K
In the case of cyanide (JIS K0102 38) and ammonia (JIS K 0102 42.2), it may be difficult to directly apply the microreactor of the present invention. The reason is that the distillation is required in the pretreatment stage and vapor generated during the distillation cannot escape. In this case, the microreactor of the present invention can be applied by using a sample that has been subjected to an external distillation operation in advance as a sample to be measured.

[0056]

EXAMPLE As an experimental example using the microreactor shown in FIG. 6, the results of the calibration curve data of hexavalent chromium are shown in Table 1. FIG. 7 shows a graph of Table 1.

[0057]

[Table 1]

As described above, in the experimental example in which 200 microliters of the test solution was stored in the measurement cell part, the correlation function R of the calibration curve was obtained.
² shows 0.9759, and it was found that the present invention can be sufficiently put to practical use as a calibration curve for the purpose of simple and quick analysis. In addition, when it is desired to perform an analysis that secures accuracy even if it takes time, R ² is at least 0.99 or more, preferably 0.998 or more.

It can be expected that even a trace amount of analysis will be possible by reducing the amount of test solution, but when a calibration curve was actually created with 100 microliters, the correlation coefficient R ² was 0.8.
It became 651. This is an accuracy that cannot be put to practical use. Further, when the test solution was 50 microliters, the effect of surface tension was relatively large, and the optical path length could not be kept uniform, and measurement could not be performed. On the contrary, although it can be expected that the accuracy of analysis will be improved by increasing the amount of test liquid, since the full capacity of the measuring cell unit 14 shown in FIG. 6 is 251 microliters, 200 microliters of the experimental example is a practical upper limit. Is considered to be.

[0060]

According to the present invention, it is possible to provide a microreactor capable of analyzing water quality simply and quickly.
Further, according to the present invention, it is possible to provide a microreactor and a water quality analyzer capable of performing water quality analysis even if a measurement sample is a trace amount.

[Brief description of drawings]

FIG. 1 is a perspective view showing an appearance of a microreactor according to the present invention.

2A is a plan view of the microreactor shown in FIG. 1, FIG. 2B is a sectional view taken along line I-I of FIG. 2A, and FIG. 2C is a sectional view taken along line II-II of FIG. 2A.

FIG. 3 is a perspective view showing the appearance of a microreactor having two series according to the present invention.

FIG. 4 is a perspective view showing an appearance of a microreactor having three series according to the present invention.

FIG. 5 is a perspective view showing an appearance of a microreactor that is preferably used for continuous operation according to the present invention.

FIG. 6A is a plan view of a microreactor suitably used for chlorine analysis according to the present invention, and FIG. 6B is I of FIG.
It is a II-III sectional view.

FIG. 7 is a graph showing a spectroscopic analysis result when chlorine analysis is performed using the microreactor shown in FIG.

[Explanation of symbols]

2 substrates 4,4 ', 4 "sample supply unit 6,6 ', 6 "supply section 8,8 ', 8 "supply section 10, 10 ', 10 "solvent supply section 12,12 ', 12 "flow path 14,14 ', 14 "measuring cell section 16 mixing section 18 Coloring part 22,24 heater 26 cooling sections 28,30 filters 34 Supply Department 36 Supply Department 38 Supply Department 40 Supply Department 42 discharge channel

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) G01N 21/77 G01N 21/77 B 31/20 ZAB 31/20 ZAB 33/18 106 33/18 106Z // G01N 21/27 21/27 Z F term (reference) 2G042 AA01 BB16 BB17 BB18 BC06 CA02 CB03 DA08 EA03 EA07 FA01 FA11 FB02 GA01 GA04 HA02 HA07 2G054 AA02 AB10 BA01 BB02 CA10 EA06 GB01 2G057 A05 BB01 BA03 AB01 AB01 AB01 AB01 AB06 AB06 EA06 JA20 2G059 AA01 AA05 BB04 DD12 DD16 EE01 EE06 FF08 FF12 HH02 HH06 JJ01 LL03 MM12 MM15 4G075 AA13 AA65 BB05 BD15 CA02 EB01 ED15 FB06 FB12

Claims (11)

[Claims] 1. A substrate, a one or more supply unit provided on the substrate, a measurement cell unit provided on the substrate, and a flow path connecting the supply unit and the measurement cell unit. Microreactor for water quality analysis. 2. The microreactor for water quality analysis according to claim 1, wherein the flow path has a width of 50 micrometers or more and 500 micrometers or less. 3. The water quality analyzer according to claim 1, comprising two or more series including one or more of each of the supply unit, the measurement cell unit, and the flow channel. Micro reactor. 4. The microreactor for water quality analysis according to claim 1, wherein one or more solid-liquid separation means is provided in the middle of the flow path. 5. The microreactor for water quality analysis according to claim 1, further comprising one or more heating means in the middle of the flow path. 6. The microreactor for water quality analysis according to claim 5, wherein a shaker is used as a part of the heating means. 7. The one of the supply units is disposed inside the substrate, and the rest of the supply unit and the measurement cell unit are disposed on the surface of the substrate. 7. The microreactor for water quality analysis according to 5 or 6. 8. The microreactor for water quality analysis according to claim 1, wherein the supply unit and the measurement cell unit are arranged open to the outside of the substrate. . 9. The microreactor for water quality analysis according to claim 1, wherein the flow path is connected to the lowermost portion of the measurement cell section. 10. A water quality analyzer comprising the water quality analysis microreactor according to claim 1 and a spectroscopic analyzer. 11. The water quality analyzer according to claim 10, further comprising data output means connected to the spectroscopic analyzer.