JP2003176218A - Skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation exhibiting ameliorating and prophylactic effects on dry skin, wrinkles and rough skin through improving the epidermal barrier function of the skin and the moisture regain condition of stratum corneum. <P>SOLUTION: This skin care preparation is obtained by formulating a combination of hydrolyzed silk proteins with seaweed extract. Further, combined use of at least one ingredient selected from licorice extract, mulberry extract and burnet extract can impart the skin with transparent feeling. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for the skin, which is effective for improving and preventing dry skin, wrinkles, and rough skin by restoring the epidermal barrier function of the skin and improving the water environment of the stratum corneum. Regarding

[0002]

The stratum corneum, which is the outermost layer of the skin, functions as a defense wall against external stimuli (ultraviolet rays, dryness, etc.). For example, it is known that the stratum corneum has both an action of suppressing the release of water from the body and an action of retaining the water of the stratum corneum itself in order to protect oneself from the dryness of the external environment. In addition, the decrease in water content in the stratum corneum causes the stratum corneum to become stiff and crack, causing wrinkles, facilitating the entry of external factors (such as antigens), and inducing a skin inflammatory reaction. is there. So far, in order to improve the stratum corneum with reduced water content, oils and natural moisturizing factors such as sugar, amino acids, lactic acid, pyrrolidone carboxylate,
Improvement has been attempted by blending and applying a moisturizing agent such as a protein such as collagen, a polysaccharide such as hyaluronic acid and chondroitin sulfate, and a polyhydric alcohol such as glycerin and 1,3-butylene glycol to a skin external preparation. Furthermore, in recent years, there is an increasing tendency to demand such ingredients from plants, reflecting the natural and plant orientations of consumers.

However, among the above-mentioned components of plant origin which have already been reported, since the activity is low, it takes a considerably high concentration to obtain a sufficient action and effect when incorporated into an external preparation for skin, and it is stable. All of the stability and safety of the formulation and the action and effect when blended in a skin external preparation, such as those that have problems in terms of safety and safety, and those that impart an undesirable color or odor to the skin external preparation. In the present situation, there are few things that can be satisfied.

[0004]

Therefore, in the present invention, by repairing the epidermal barrier function of the skin and improving the water environment of the stratum corneum, improvement of dry skin, wrinkles, and rough skin,
It was also intended to obtain an external preparation for the skin, which is effective for prevention.

[0005]

[Means for Solving the Problems] Various investigations have been made to solve the above-mentioned problems. As a result, the combined use of hydrolyzed silk protein and seaweed extract in an external preparation for skin has the effect of improving the epidermal barrier function of the skin. By repairing and improving the moisture environment of the stratum corneum, improvement of dry skin, wrinkles, and rough skin,
And it was found to be effective in prevention.

Further, it has been found that a transparent feeling can be imparted to the skin by using in combination with one or more kinds selected from the licorice extract, the ware extract and the mulberry extract.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described.

The hydrolyzed silk protein used in the present invention is obtained by hydrolyzing silk protein by acid, alkali or enzyme treatment. The silk protein is a mixture of proteins containing fibroin and sericin as main components, and in the present invention, fibroin and sericin can be used alone or as a mixture of hydrolyzed fibrin and sericin. It is preferable to use hydrolyzed sericin from the viewpoints of improving rough skin and preventing it.

In the present invention, the hydrolyzed sericin may be one obtained by partially hydrolyzing sericin contained in silkworm cocoons or raw silk in an acidic or alkaline aqueous solution or by enzymatic treatment and eluting. The pH of this eluate is adjusted to 3 to 5 with an organic acid or an inorganic acid, or a water-soluble organic solvent such as methanol, ethanol, or dioxane is mixed to precipitate hydrolyzed sericin. It is also possible to use the prepared one. Further, the hydrolyzed sericin used in the present invention has an average molecular weight of about 10,000.
It is particularly preferably from 0 to 200,000 and containing from 20 to 40 mol% of serine.

The hydrolyzed sericin used in the present invention is
It is easily water-soluble, has excellent miscibility with usual skin external preparation components, maintains stable properties as an aqueous solution, has no irritation and sensitization to the skin, and is excellent in safety.

The amount of the hydrolyzed silk protein in the present invention to be added to the external preparation for skin can be adjusted depending on the dosage form of the external preparation for skin and the purpose, but a suitable amount is based on the total amount of the external preparation for skin. , 0.0001 to 20% by weight, more preferably 0.1 to 10% by weight.

As the effect of the hydrolyzed sericin on the skin, a skin inflammation-preventing effect (Japanese Patent Laid-Open No. 24534/1998) is used.
5), tyrosinase activity inhibitory effect (JP-A-10-265)
403), surface activity (JP-A-11-276876),
Collagen production promoting effect (JP-A-10-22665)
3), substance P antagonism (Japanese Patent Application Laid-Open No. 10-1581)
77) and the like, and further, the combined use with yeast extract (JP-A-11-193210), the combined use with monosaccharides and oligosaccharides (JP-A-2001-64148) and the like are also disclosed.

In the present invention, it has been found that the combined use of the hydrolyzed silk protein and the seaweed extract exerts a high effect of repairing the epidermal barrier function and an effect of improving the water environment of the stratum corneum. It was a completely new effect that was unpredictable from the technology.

Next, in the present invention, any of green algae, red algae, and brown algae can be used as seaweeds, but it is preferable to use red algae, and particularly seaweeds belonging to Gigartinales are preferably used. .
In the order of Scutellaria, seaweeds such as Scutellariae, Scutellariae, Scutellariae, Scutellariae, Eucalyptus, Pheasantaceae, Acanthaceae, Pleurotus spp. Gigartina tenella Harvey), Kainori ( Gigartin
a intermedia Suringar), Gigartina tee
dii Lamouroux), Ibonori ( Gigartina pacifica Kjel
lman), Squid reed ( Gigartina mamillosa J. Agard
h), tsunomata ( Chondrus ocellatus Holmes), hirakotoji ( Chondrus pinnulatus Okamura), thorny nomata ( Chondrus armatus Okamura), kotojitsunomata ( Chon )
drus elatus Holmes), Black-spotted Ginseng ( Chondrus
yendoi Yamada et Mikami), Nanha Sou ( Rh
odoglossum pulcherum Setchell et Gardner), Ogonori ( Gracilaria verrucosa Papenfuss), Ogonori ( Gracilaria gigas Harvey), Silamo ( Gracilaria bu )
rsa- pastris Silva), Tsurcilamo ( Gracilariopsis ch )
orda Ohmi), Gracilaria incurvata Ok
amura） 、 Cavanori ( Gracilaria textorii Suringa
r), Yumigatatagonori ( Gracilaria arcuata Zanardin
i), Ryukyugogonori ( Gracilaria eucheumoides)
Harvey), Fushikrenori ( Corallopsis opuntia J. Ag
ardh), sperm ( Ceratodictyon spongiosum Zan
ardini), Japanese oyster mushroom ( Tylotus li chenoides Okamur)
a), Isomokka ( Catenella opuntia Greville), Usuginu ( Nemastoma lancifolia Okamura), Honoo ( Platoma japonica Okamura), Venice Nago ( Schizyme )
nia dubyi J. Agardh), Yukari ( Plocamium telfairia )
e Harvey), Soybean ( Plocamium leptophyllum var.
flexuosum J. Agardh), Maki Yukari ( Plocamium recu )
rvatum Okamura), Hime Yukari ( Plocamium oviforme O
kamura), Isodantsu ( Caulacanthus okamurai Yama
da), Kijinoo (Phacelocarpus japonicus Okamur
a), Mirin ( Solieria robusta Kylin), Hosobamirin ( Solieria mollis Kylin), Ezonameshi ( Turnerell )
a mertensiana Schmitz), Tosakanori ( Meristotheca p
apulosa J. Agardh), Meristotheca coac
ta Okamura), Giraffe ( Eucheuma muricatum Weber)
van Bosse), Eucheuma amakusa
nensis Okamura), Togekirinsai ( Eucheuma serra)
J. Agardh), juniper giraffe ( Eucheuma cupres)
oideum Weber van Bosse), Suscarveni ( Halarachni )
on latissimum Okamura), Ibaranori ( Hypnea charoi
des Lamouroux), Kagii Baranori ( Hypnea japonica T
anaka), Saidaibara (Hypnea saidana Holmes), Tachiibaranori (Hypnea variabilis Okamura), Himoibara (Hypnea chordacea Kuetzing), rerun (Ahnfelt
ia concinna J. Agardh), Aegness ( Ahnfeltia pli )
cata F. Fries), Harigane ( Ahnfeltia paradoxa Okam
ura), Okitsunori (Gymnogongrus flabelliformis Har
vey), Maguera ( Trematocarpus pygmaeus Yend
o), Atsuno Nori ( Sarcodia ceylanica Harvey), Lotus japonicus ( Stenogramma interrupta Montagne), Euchakimadara ( Fauchea stipitata Ymamada et Segawa), Weebagsa ( Webberella micans Hauptfleisch) and the like. Among the seaweeds mentioned above, from the viewpoint of improving dry skin, wrinkles, rough skin, and preventing effect, simi ( Ahnfeltia concin
most preferably na J. Agardh).

Although the seaweed may be used for extraction as it is,
Considering the extraction efficiency, it is preferable to carry out the extraction after performing processing such as shredding, drying and crushing. The extraction is performed by immersing it in an extraction solvent. Stirring to improve extraction efficiency,
It may be homogenized in an extraction solvent or may be hydrolyzed by enzymatic treatment or the like. As the extraction temperature, it is appropriate to set the temperature to about 5 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time depends on the type of extraction solvent and the extraction temperature, but
It is appropriate that the time is about 14 days.

As the extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as diethyl ether and dipropyl ether, esters such as ethyl acetate and butyl acetate And organic solvents such as ketones such as acetone and ethyl methyl ketone can be used, and one kind or two or more kinds are selected from these and used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used.

The extract of seaweed with the above-mentioned solvent can be contained as it is in the external preparation for skin according to the present invention, but the concentrated and dried product can be redissolved in water or a polar solvent, or these physiological products can be used. Decolorization within the range that does not impair the action,
It may be used after purification such as deodorization and desalting, or fractionation such as column chromatography.
It can also be used after the extract is further fermented or subjected to a hydrolysis treatment. The above-mentioned extract of seaweed and its processed products and fractions can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use. It can also be used by encapsulating it in vesicles such as liposomes or in microcapsules.

The amount of the seaweed extract in the external preparation for skin of the present invention can be adjusted according to the dosage form of the external preparation for skin, purpose and the like, but a suitable amount is based on the total amount of the external preparation for skin. 0.001 to 20% by weight, preferably 0.0
It is 1 to 10% by weight.

As the effect of the seaweed extract on the skin, an elastase activity inhibitory effect (Japanese Patent Laid-Open No. 2001-181)
167), ultraviolet absorption effect (JP-A-8-18852)
1), antibacterial / antiseptic effect (JP-A-11-180813),
Atopic dermatitis preventive / therapeutic effect (JP-A-10-279
491), the effect of suppressing immune function (JP-A-6-29866).
1) and the like are known, and the combination use with glabridin contained in the licorice extract (Japanese Patent No. 3091045) and the combination use with collagen (JP-A 2000-229832) are also disclosed.

In the present invention, the hydrolyzed silk protein and the seaweed extract may be used in combination with one or more selected from licorice extract, oleander extract and mulberry extract. By using these components in combination, the effect of repairing the barrier function of the epidermis is improved, and the effect of imparting transparency to the skin can be exhibited.

The effects of the licorice extract on the skin include testosterone-5α-reductase inhibitory action (Japanese Patent Publication No. 4-69620) and cell activation action (Japanese Patent Publication No. 6-86).
387), melanin production inhibitory effect (Japanese Patent Publication No. 6-824)
9), antioxidative effect (* 2805325), etc., and is used in combination with an amino acid mixture selected from the group consisting of seaweed extract, yeast autolysate, silk hydrolyzate and soybean hypocotyl extract ( Japanese Patent Application No. 3091045), combined use with an anti-inflammatory agent (No. 3052104), combined use with kojic acid (No. 2549127) and the like are also disclosed. The effect of the extract of Corydalis communis on the skin includes testosterone-5α-reductase inhibitory action (Japanese Patent No. 2871763), elastase inhibitory action (Japanese Patent No. 2969451), hydroxy radical scavenging action (Japanese Patent Application Laid-Open No. 7-133216), collagenase inhibitory effect (Japanese Patent Application Laid-Open No. 7-133216). -196526), antiallergic action (JP-A-10-36276), lipolysis accelerating action (JP-A-10-158181), ceramide production accelerating action (JP-A-2000-169359), metalloprotease inhibitory action (JP-A-2000-319155). Etc. are known.
Further, as the effects of mulberry extract on the skin, whitening and anti-inflammatory effects (Fragrance Journal Extra Number No. 6, page 348), antioxidant effects (Japanese Patent Publication No. 3-7235), etc. are known. Combination of (special 295733
4) etc. are also disclosed.

The licorice extract used in the present invention is a component obtained by extracting from a licorice plant. Glycyrrhiza plant is a dicotyledonous plant belonging to the legume family (Leguminosae), especially but not limited its kind, Spain licorice (Glycyrrhiza glabra L.), Kikanzou (Glycyrrhiza
kansuensis Chang et peng), licorice ( Glycyrrhiza
urarensis Fisch.) is preferably used. The site to be used for obtaining the extract from the licorice plant is not particularly limited, but it is preferable to use the root from the viewpoint of the effect of imparting a transparent feeling to the skin. In addition, glabridin, hispaglabridin, liquiritin, etc. purified from the extract can also be used. The content of the licorice extract in the invention to the external preparation for skin can be adjusted depending on the dosage form of the external preparation for skin, purpose, etc., but a suitable amount is 0.001 to 0.001 to the total amount of external preparation for skin. It is 5% by weight.

[0023] The extract of Gypsophila vulgaris used in the present invention is of the genus Sanguisorba ( Rosaceae ).
L.) which belongs to the plant Warremoko ( Sanguisorba officinali
s L.) is the component obtained by extraction. There are no particular restrictions on the site used to obtain the wareworm extract, including leaves, stems,
Although various parts such as flowers and roots can be used, it is preferable to use the root part in terms of the effect of imparting transparency to the skin.
The amount of the oleander extract in the invention to be added to the skin external preparation can be adjusted depending on the dosage form of the skin external preparation, the purpose, etc., but a suitable amount is 0.001 to 0.001 to the total amount of the skin external preparation. It is 5% by weight.

The mulberry extract used in the present invention is a component obtained by extraction from a mulberry plant. Mulberry plants are dicotyledonous plants belonging to the family Moraceae ( Moraceaceae ), and their species are not particularly limited , but mulberry ( Morus alba L .; Morus
atropurpure Roxb.), Shimaguwa ( Morus australis Poi
r.; Morus acidosa Griff.), Yamaguchi ( Morus bombyci
s Koidz .; Morus alba L. var. stylosa Bur .; Morus
japonica Bailey non Sieb.), Hachijougua ( Morus
lagayamae Koidz.), Rowa ( Morus latifolia (Bur.) P
oir.; Morus alba L. var. latifolia Bur.; Morus m
ulticaulis Perr .; Morus alba L. var. multicaulis L
oud.), Morus mongolica (Bur.) Schneid.
Morus alba var. Mongolica Bur.), Black Migwa ( Mor
Us nigra L.), red mulberry ( Morus rubra L.), etc. are illustrated. Among these mulberry plants, the mulberry ( Morus alba L .; Morus atropurpure Rox
b.) or Yamaguchi ( Morus bombycis Koidz .; Morus
alba L. var. stylosa Bur .; Morus japonica Bailey
non Sieb.), and these cultivars for cultivation are preferably used. The site to be used for obtaining an extract from a mulberry plant is not particularly limited, but root bark, bark and leaves are preferable, and root bark is particularly preferable from the viewpoint of the effect of imparting transparency to the skin. The compounding amount of the mulberry extract in the invention in the external preparation for skin can be adjusted depending on the dosage form and the purpose of the external preparation for skin, but a suitable mixing amount is 0.0 with respect to the total amount of the external preparation for skin.
It is from 01 to 5% by weight.

A licorice extract from a licorice plant is used.
The extraction method and the extraction solvent for obtaining a waremoko extract from Pleurotus cornucopia and a mulberry extract from a mulberry plant are the same as those for the seaweed extract described above.

The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders and granules.

The external preparation for skin according to the present invention includes hydrolyzed silk protein, seaweed extract, licorice extract, oleander extract, mulberry extract, oily component, surfactant, humectant and pigment. , It may contain general pharmaceutical and cosmetic raw materials such as UV absorbers, antioxidants, fragrances, antibacterial and antifungal agents, and physiologically active ingredients such as skin cell activating agents, anti-inflammatory agents, and whitening agents. it can.

[0028]

EXAMPLES The features of the present invention will be described in detail with reference to Examples.

First, hydrolyzed silk protein contained in the external preparation for skin according to the present invention and its aqueous solution, seaweed extract,
Preparation of licorice extract, oleander extract and mulberry extract is shown below.

[Hydrolyzed Silk Protein] Silkworm cocoons are treated with a bacterial alkaline protease derived from Bacillus licheniformis at pH 8.0 and 55 ° C. for 1 hour to elute hydrolyzed sericin. The eluate was concentrated under reduced pressure, 2 volumes of ethanol was added to precipitate hydrolyzed sericin, which was separated by filtration and dried to obtain hydrolyzed silk protein containing hydrolyzed sericin as a main component. The average molecular weight of this hydrolyzed silk protein is about 13,000.
It contained 35 mol% of serine.

[Hydrolyzed Silk Protein Aqueous Solution] 5 g of the hydrolyzed silk protein was dissolved in 95 g of purified water to obtain a hydrolyzed silk protein aqueous solution.

[Seaweed extract] 2.4 kg of algal cells of seaweed ( Ahnfeltia concinna J. Agardh), which is a seaweed belonging to the family Asteraceae , is dried and then pulverized, immersed in 1.5 liters of purified water, and 50 ° C to 60 ° C. The mixture was extracted with stirring at 0 ° C for 10 hours. The extract was filtered and the filtrate was collected as a seaweed extract.

[Licorice extract] Spanish licorice ( Glycyr
(rhiza glabra L.) 500 g of roots and rhizomes are dried and pulverized, and the mixture is placed in 1,000 ml of anhydrous ethanol solution at 25 ° C.
The mixture was extracted with stirring for 24 hours. The extract is filtered to collect the filtrate, concentrated under reduced pressure, and then freeze-dried to dryness. The dried solid was extracted with stirring in 1,000 ml of ethyl acetate at 20 ° C. for 2 days. After extraction and drying under reduced pressure, 5 g of the obtained extract was dissolved in 500 ml of 95% by volume aqueous ethanol solution to give a licorice extract.

[ Walemokou extract] Waremokou ( Sangui
350 g of roots of sorba officinalis L.) are dried and pulverized, immersed in 1.5 liter of 50% by volume ethanol aqueous solution at 20 ° C. for 7 days, and then filtered to collect a filtrate,
It was used as a Waremoko extract.

[Mulberry Extract] 350 g of mulberry ( Morus alba L.) root bark was finely chopped and immersed in 1,000 ml of a 50% by volume aqueous 1,3-butylene glycol solution at 20 ° C. for 7 days for extraction. . The extract was filtered and the filtrate was collected as a mulberry extract.

Next, the formulations of the examples of the present invention in which the above-mentioned extract is mixed with the external preparation for skin are shown.

According to the formulation shown in Table 1, Example 1 of the present invention
~ A skin lotion according to Example 4 was prepared. At the same time, Comparative Examples 1 to 3 in which either one or both of the hydrolyzed silk protein and the seaweed extract were not mixed were prepared according to the formulations shown in Table 2. The lotion for skin was prepared by sequentially adding all the components to purified water, mixing and homogenizing.

[0038]

[Table 1]

[0039]

[Table 2]

The skin lotions shown in Tables 1 and 2 were subjected to usage tests to confirm the effects of the present invention. In the use test, 20 female panelists having symptoms of dryness, wrinkles, and rough skin were made into one group, and each of the groups was treated with Example 1 to Example 4 and Comparative Example 1 to Comparative Example 3 twice a day by blinds. I used it for a month.

The skin transepidermal water loss (TEWL), water content, wrinkles, rough skin, and the feeling of transparency before and after the use test were compared, and "improvement" was performed on these improvement conditions.
It was evaluated in three grades of "Slightly improved" and "No change". When the number of panelists evaluated as improved was 15 or more, "◎", when 10 to 14 were "○", 5-9. The results are shown in Table 4 as “Δ” for the case of first name and “×” for the case of 0 to 4 people. In addition, TEWL is TEVAMETE
R TM210, Integral Co., Ltd. in Japan)
The skin moisture content is measured by a skin moisture meter (SKICON-
200, manufactured by IBS) at room temperature of 23
The measurement was carried out in a constant temperature and humidity room at a temperature of 55 ° C and a humidity of 55%. The condition of wrinkles, rough skin, and transparency was evaluated by visualizing the skin conditions before and after use as images in a computer and comparing them visually.

[0042]

[Table 3]

As is clear from Table 3, in the examples of the present invention, a clear improvement was observed in TEWL, which is an index of the epidermal barrier function, and the water content was increased to improve the dryness of the skin. Was shown. Furthermore, the effect of improving wrinkles and rough skin was also recognized. Further, in addition to the hydrolyzed silk protein aqueous solution and the seaweed extract, licorice extract, oleander extract and mulberry extract were added, respectively.
In, the effect of improving the transparency was also recognized. On the other hand, in Comparative Example 1 and Comparative Example 2 which did not contain either the hydrolyzed silk protein aqueous solution or the seaweed extract, although the improvement tendency of the epidermal barrier function was recognized, the extent of the hydrolyzed silk protein It was lower than that of the example in which the aqueous solution and the seaweed extract were used in combination.

Another embodiment of the present invention will be described.

Example 5 Lotion (1) Sodium dl-pyrrolidonecarboxylate (50% by weight aqueous solution) 0.5 (% by weight) (2) Citric acid 0.1 (3) Sodium citrate 0.1 ( 4) Sodium lactate 0.1 (5) Purified water 69.45 (6) 1,3-Butylene glycol 2.0 (7) Concentrated glycerin 4.0 (8) Methyl paraoxybenzoate 0.2 (9) Ethanol 4 .0 (10) Carboxyvinyl polymer (1% by weight aqueous solution) 10.0 (11) Licorice extract 0.1 (12) Mulberry extract 0.2 (13) Novara oil 0.05 (14) Warmout extract 0 2 (15) Seaweed extract 2.0 (16) Hydrolyzed silk protein aqueous solution 7.0 Production method: After mixing and dissolving the components of (1) to (5), (6) to (16)
The ingredients are added sequentially to dissolve and homogenize.

Example 6 Emulsion (1) Stearic acid 1.0 (wt%) (2) 2-Hexyldecyl isostearate 1.0 (3) Lipophilic glyceryl monostearate 0.2 (4) Salasi Beeswax 0.2 (5) d-δ-tocopherol 0.01 (6) Behenyl alcohol 0.1 (7) Macadamian nut oil 1.0 (8) Squalane 2.0 (9) Novara oil 0.1 (10) 1,3-Butylene glycol 10.0 (11) Potassium hydroxide 0.2 (12) Sucrose stearate 0.1 (13) Purified water 40.61 (14) Carboxyvinyl polymer (5% by weight aqueous solution) 10 0.0 (15) N-stearoyl-L-glutamate sodium 0.05 (16) L-arginine 0.03 (17) Seaweed extract 10.0 (18) Concentrated glycerin 15.0 (19) dl-pyrrolidone carboxylic acid Sodium (50% by weight aqueous solution) 1.0 (20) Ethanol 2.0 (21) Licorice Outcome 0.1 (22) Methyl paraoxybenzoate 0.2 (23) Waremoko extract 0.1 (24) Hydrolyzed silk protein aqueous solution 5.0 Production method: (1) to (9) oil phase components and ( The aqueous phase components of 10) to (19) are heated and dissolved at 85 ° C., respectively. The oil phase component is added to the water phase component and emulsified using a homomixer. The mixture is cooled to 50 ° C., and the ingredients (20) to (24) are sequentially added, mixed and homogenized.

[Example 7] Oil-in-water emulsion type cream (1) Stearic acid 2.0 (% by weight) (2) 2-hexyldecyl isostearate 10.0 (3) Lipophilic type glyceryl monostearate 1.0 (4) Behenyl alcohol 2.5 (5) Sarah beeswax 2.0 (6) Macadamia nut oil 2.0 (7) Olive oil 2.0 (8) Decamethylcyclopentasiloxane 2.0 (9) d-δ-tocopherol 0 0.05 (10) 1,3-butylene glycol 3.0 (11) potassium hydroxide 0.3 (12) sucrose myristic acid ester 0.8 (13) carboxyvinyl polymer (3% by weight aqueous solution) 10.0 ( 14) Sodium N-stearoyl-L-glutamate 0.5 (15) Purified water 49.827 (16) Ethanol 4.0 (17) Citric acid 0.003 (18) Sodium citrate 0.01 (19) Hydrolysis Silk protein aqueous solution 5.0 (20) Hydrogenation Soybean phospholipid 0.7 (21) Licorice extract 0.1 (22) Fireweed extract 0.2 (23) Seaweed extract 2.0 (24) Fragrance 0.01 Manufacturing method: (1) to (9) The oil phase component and the aqueous phase components (10) to (15) are heated and dissolved at 85 ° C. The oil phase component is added to the water phase component and emulsified using a homomixer. The mixture is cooled to 50 ° C., and the components (16) to (24) are sequentially added and mixed and homogenized.

Example 8 Water-in-oil emulsion type emollient cream (1) Liquid paraffin 30.0 (wt%) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglycerin oleic acid Ester 5.0 (5) L-Glutamic acid sodium 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) 1,3-Butylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 (10) Seaweed extract 1.0 (11) Fireweed extract 0.5 (12) Hydrolyzed silk protein aqueous solution 1.0 (13) Purified water 44.8 (14) Perfume 0.1 (15) Liquiritin 0.02 (16) Ethanol 0.48 Manufacturing method: (5), (6) was dissolved in a part of (13) to 50 ° C.,
Gradually add to (4) heated to 50 ° C. with stirring.
This was mixed in advance and dissolved by heating at 70 ° C (1) ~
Disperse evenly in (3), add (7) to (12) to the rest of (13) and heat to 70 ° C. with stirring,
Emulsify with a homomixer. After cooling, at 40 ℃ (14) ~
Add the component (16) and mix.

[Example 9] Makeup base cream (1) Stearic acid 12.0 (% by weight) (2) Licorice extract 0.1 (3) Cetanol 2.0 (4) Glycerin tri-2-ethylhexane Acid ester 2.5 (5) Self-emulsifying glycerin monostearate ester 2.0 (6) Propylene glycol 11.0 (7) Potassium hydroxide 0.3 (8) Seaweed extract 1.0 (9) Warmoko extract Material 0.5 (10) Hydrolyzed silk protein 1.0 (11) Purified water 66.0 (12) Titanium oxide 1.0 (13) Red iron oxide 0.1 (14) Yellow iron oxide 0.4 (15) Fragrance 0.1 Manufacturing method: The oil phase components (1) to (5) are mixed and heated to 75 ° C. to make the mixture uniform. On the other hand, by mixing the water phase components (6) to (11),
It is heated to 75 ° C. and melted to make it uniform, and the pigments (12) to (14) are added thereto and dispersed uniformly by a homomixer. The oil phase component is added to the aqueous phase component, the mixture is emulsified by a homomixer and then cooled, and the component (15) is added and mixed at 40 ° C.

[Example 10] Milk liquid foundation (1) Stearic acid 2.0 (wt%) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglycerin monoisopalmitate 9.0 (6) Macadamia nut fatty acid phytosteryl 0.5 (7) Licorice extract 0.1 (8) 1,3-Butylene glycol 8.0 (9) Potassium hydroxide 0.1 ( 10) Methyl paraoxybenzoate 0.1 (11) Purified water 48.5 (12) Titanium oxide 9.0 (13) Talc 7.4 (14) Red iron oxide 0.5 (15) Yellow iron oxide 1.1 (16) ) Black iron oxide 0.1 (17) Perfume 0.1 (18) Seaweed extract 1.0 (19) Warmoko extract 0.5 (20) Hydrolyzed silk protein 1.0 Manufacturing method: (1) to (7) ) Are mixed and heated to 75 ° C. to make the mixture uniform. On the other hand, by mixing the water phase components (8) to (11),
Heat to 75 ° C to dissolve and homogenize, add the pigments of (12) to (16) to this, homogenize with a homomixer and then cool, and cool to 40 ° C at (17) to (20). Add and mix the ingredients.

Example 11 Hand Cream (1) Cetanol 4.0 (wt%) (2) Vaseline 2.0 (3) Liquid Paraffin 10.0 (4) Glycerin Monostearate 1.5 (5) Polyoxyethylene (60EO) glycerin isostearic acid ester 2.5 (6) Licorice extract 0.1 (7) Tocopherol acetate 0.5 (8) Soybean phospholipid 0.5 (9) Glycerin 20.0 (10) Paraoxy Methyl benzoate 0.1 (11) Purified water 56.3 (12) Seaweed extract 1.0 (13) Warmoko extract 0.5 (14) Hydrolyzed silk protein 1.0 Manufacturing method: (1) to (7) The oil phase components of 1) are mixed, dissolved and heated to 75 ° C. On the other hand, the water phase components (8) to (11) are mixed, dissolved and heated to 75 ° C. Next, the oil phase component is added to the aqueous phase component to pre-emulsify it, then the mixture is uniformly emulsified with a homomixer and cooled, and the components (12) to (14) are added and mixed at 40 ° C.

Regarding Example 5 to Example 11 of the present invention,
A usage test was conducted in the same manner as in Examples 1 to 4. The results are shown in Table 4.

[0053]

[Table 4]

As shown in Table 4, in the examples of the present invention, TEWL, which is an index of the epidermal barrier function, was also improved, the water content was increased, and the dry condition of the skin was improved. Furthermore, it was shown that wrinkles, rough skin, and transparency were improved, and high effectiveness was exhibited. No panelists complaining of skin irritation were observed during the use test period, indicating that the safety was good. Furthermore, during the use test period, no change in the state of the preparation such as precipitation, separation, aggregation, discoloration or discoloration of the contained components was observed.

[0055]

As described in detail above, according to the present invention, the epidermal barrier function of the skin is restored and the water environment of the stratum corneum is improved, thereby improving and preventing dry skin, wrinkles, and rough skin. It was possible to obtain a skin external preparation that was effective.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Ichiro Sonoda             112-1 Okada-cho, Yoka-shi, Shiga Co., Ltd.             Noevir Shiga Institute (72) Inventor Masafumi Kawauchi             6-13-1, Minatojima Nakamachi, Chuo-ku, Kobe City, Hyogo Prefecture             Noevir Kobe Head Office (72) Inventor Hidekazu Shiomi             1-10-1 Koya, Fukui City, Fukui Prefecture Sale             Within the corporation (72) Inventor Hideyuki Yamada             1-10-1 Koya, Fukui City, Fukui Prefecture Sale             Within the corporation F-term (reference) 4C083 AA082 AA111 AA112 AA122                       AB032 AB232 AB242 AB432                       AC012 AC022 AC072 AC102                       AC122 AC242 AC302 AC352                       AC422 AC482 AC582 AC612                       AC662 AD092 AD172 AD222                       AD282 AD451 AD452 AD492                       AD572 AD662 CC02 CC04                       CC05 CC11 CC12 DD32 DD33                       EE06 EE12

Claims (5)

[Claims] 1. A skin external preparation containing a hydrolyzed silk protein and a seaweed extract. 2. The external preparation for skin according to claim 1, wherein the hydrolyzed silk protein is hydrolyzed sericin. 3. The external preparation for skin according to claim 1, wherein the seaweed extract is a red alga extract. 4. The external preparation for skin according to claim 3, wherein the red alga extract is a red seaweed Cynophoraceae Cynomaceae extract. 5. The external skin preparation according to claim 1, further comprising one or more selected from licorice extract, oleander extract and mulberry extract.