IN2015DN02479A - - Google Patents

Info

Publication number
IN2015DN02479A
IN2015DN02479A IN2479DEN2015A IN2015DN02479A IN 2015DN02479 A IN2015DN02479 A IN 2015DN02479A IN 2479DEN2015 A IN2479DEN2015 A IN 2479DEN2015A IN 2015DN02479 A IN2015DN02479 A IN 2015DN02479A
Authority
IN
India
Prior art keywords
nucleic acids
biological sample
target nucleic
provides methods
present
Prior art date
Application number
Inventor
Viswanathan; Srinivasan
Donald; Cullis
Huyet; Luu
Jorge A ; Rodriguez
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Publication of IN2015DN02479A publication Critical patent/IN2015DN02479A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Communicable Diseases (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides methods of extracting target nucleic acids from a biological sample using divalent salts and acidic conditions.
IN2479DEN2015 2012-09-19 2013-09-18 IN2015DN02479A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261703112P 2012-09-19 2012-09-19
PCT/US2013/060349 WO2014047141A1 (en) 2012-09-19 2013-09-18 USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS

Publications (1)

Publication Number Publication Date
IN2015DN02479A true IN2015DN02479A (en) 2015-09-11

Family

ID=49304332

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2479DEN2015 IN2015DN02479A (en) 2012-09-19 2013-09-18

Country Status (10)

Country Link
US (1) US20140087366A1 (en)
EP (1) EP2898069B1 (en)
JP (1) JP2015528313A (en)
KR (1) KR20150054844A (en)
CN (1) CN105008534A (en)
AU (1) AU2013318156A1 (en)
BR (1) BR112015005718A2 (en)
IN (1) IN2015DN02479A (en)
SG (1) SG11201502138WA (en)
WO (1) WO2014047141A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10233440B2 (en) 2012-09-28 2019-03-19 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
CN104749009B (en) * 2015-03-30 2018-05-04 上海云泽生物科技有限公司 immunosuppressant drug extraction reagent for immunoassay
KR101927720B1 (en) * 2017-01-04 2018-12-11 국민대학교산학협력단 A composition for extrating microorganism in filter, and method for separation of microorganism in food using the same
CN108531472B (en) * 2017-03-05 2021-07-16 北京新羿生物科技有限公司 Lysis solution for nucleic acid extraction
WO2018222709A2 (en) 2017-06-01 2018-12-06 Nantomics, Llc Dna stabilization of rna
CN107254463B (en) * 2017-06-20 2018-11-20 迈克生物股份有限公司 For extracting the kit of HCV RNA
CN109306349A (en) * 2017-07-26 2019-02-05 天津迦美惠众科技有限公司 A kind of biological sample nucleic acid extraction purification process based on nanometer magnetic bead
CN107794260A (en) * 2017-11-21 2018-03-13 苏州吉玛基因股份有限公司 A kind of method that free nucleic acid is extracted in the acellular body fluid from large volume
US20220090048A1 (en) * 2019-01-25 2022-03-24 Sekisui Chemical Co., Ltd. Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
CN111154753A (en) * 2020-03-09 2020-05-15 重庆市大足区人民医院 Magnetic bead method bacterial nucleic acid extracting solution
KR20220075978A (en) * 2020-11-30 2022-06-08 (주)바이오니아 Method of Separating Nucleic Acid by Using Binding Buffer with Chemicals Having Low-Mid Dielectric Constant
WO2023150710A1 (en) * 2022-02-05 2023-08-10 Becton, Dickinson And Company Non-opaque lytic buffer composition formulations
CN114410747A (en) * 2022-03-30 2022-04-29 北京中海生物科技有限公司 Virus hands-free reagent and application thereof in virus genome amplification

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1301606C (en) * 1986-05-02 1992-05-26 David H. Gillespie Chaotropic method for evaluating nucleic acids in a biological sample
JP3856174B2 (en) * 1997-12-11 2006-12-13 東洋紡績株式会社 Extraction and purification method of plant DNA
WO2001021830A1 (en) * 1999-09-24 2001-03-29 Ambion, Inc. Nuclease inhibitor cocktail
US20030203384A1 (en) * 2002-03-08 2003-10-30 Chafin David R. Multiplex detection of biological materials in a sample
US7998699B2 (en) * 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
EP1559783A1 (en) * 2004-01-29 2005-08-03 Qiagen GmbH Method for the chromatographic separation of nucleic acid mixtures
KR20070097430A (en) * 2004-11-05 2007-10-04 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. Compositions and methods for purifying nucleic acids from stabilization reagents
CN101400689A (en) * 2006-02-08 2009-04-01 鲁米金公司 Methods of extracting RNA
US20070185322A1 (en) * 2006-02-08 2007-08-09 Nexgen Diagnostics Llc Methods of extracting RNA
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
EP1911844A1 (en) * 2006-10-10 2008-04-16 Qiagen GmbH Methods and kit for isolating nucleic acids
CA2720932A1 (en) * 2008-01-24 2009-07-30 Bioventures, Inc. Use of nucleic acid probes to detect nucleotide sequences of interest in a sample
JP5641191B2 (en) * 2010-02-26 2014-12-17 Jsr株式会社 Virus detection method
ES2571104T3 (en) * 2009-11-09 2016-05-24 Streck Inc Stabilization of RNA and extraction of RNA present in intact cells within a blood sample
CN102725407B (en) * 2010-01-07 2016-09-14 比格科技私人有限公司 Separate method and the test kit thereof of nucleic acid
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
DE112011102524T5 (en) * 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Preparation of generic samples

Also Published As

Publication number Publication date
BR112015005718A2 (en) 2017-07-04
JP2015528313A (en) 2015-09-28
SG11201502138WA (en) 2015-05-28
KR20150054844A (en) 2015-05-20
WO2014047141A1 (en) 2014-03-27
AU2013318156A1 (en) 2015-03-19
US20140087366A1 (en) 2014-03-27
EP2898069A1 (en) 2015-07-29
CN105008534A (en) 2015-10-28
EP2898069B1 (en) 2018-04-11

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