IN2012DN01521A - - Google Patents

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Publication number
IN2012DN01521A
IN2012DN01521A IN1521DEN2012A IN2012DN01521A IN 2012DN01521 A IN2012DN01521 A IN 2012DN01521A IN 1521DEN2012 A IN1521DEN2012 A IN 1521DEN2012A IN 2012DN01521 A IN2012DN01521 A IN 2012DN01521A
Authority
IN
India
Prior art keywords
host
schizosaccharomyces pombe
transformant
inactivated
deleted
Prior art date
Application number
Inventor
Futoshi Hara
Hideki Tohda
Yuko Hama
Original Assignee
Asahi Glass Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Glass Co Ltd filed Critical Asahi Glass Co Ltd
Publication of IN2012DN01521A publication Critical patent/IN2012DN01521A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced into Schizosaccharomyces pombe as a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated.
IN1521DEN2012 2009-08-21 2010-08-17 IN2012DN01521A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2009192271 2009-08-21
PCT/JP2010/063888 WO2011021629A1 (en) 2009-08-21 2010-08-17 Transformant and process for production thereof, and process for production of lactic acid

Publications (1)

Publication Number Publication Date
IN2012DN01521A true IN2012DN01521A (en) 2015-06-05

Family

ID=43607080

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1521DEN2012 IN2012DN01521A (en) 2009-08-21 2010-08-17

Country Status (7)

Country Link
US (1) US9284561B2 (en)
EP (1) EP2468860B1 (en)
JP (1) JP5772594B2 (en)
KR (1) KR20120051705A (en)
CN (1) CN102498209A (en)
IN (1) IN2012DN01521A (en)
WO (1) WO2011021629A1 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103392004A (en) * 2011-02-21 2013-11-13 旭硝子株式会社 Method for producing lactic acid
WO2014030655A1 (en) 2012-08-24 2014-02-27 旭硝子株式会社 Transformant and method for producing same, and method for producing lactic acid
WO2014156998A1 (en) * 2013-03-28 2014-10-02 旭硝子株式会社 Process and device for producing chemical product
RU2539092C1 (en) * 2013-09-27 2015-01-10 Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГУП "ГосНИИгенетика") RECOMBINANT STRAIN OF YEASTS Schizosaccharomyces pombe - PRODUCENT OF LACTIC ACID
ES2719828T3 (en) * 2013-11-22 2019-07-16 Jmtc Enzyme Corp Transformant and procedure for the production thereof, and procedure for the production of lactic acid
JP6488666B2 (en) * 2013-11-22 2019-03-27 Ａｇｃ株式会社 Cloning vector, expression vector and method for producing transformant
EP2977471A1 (en) * 2014-07-23 2016-01-27 PURAC Biochem BV Genetic modification of (S)-lactic acid producing thermophilic bacteria
JP2017205019A (en) * 2014-09-19 2017-11-24 旭硝子株式会社 Method for producing organic acid
JP6620375B2 (en) * 2014-10-10 2019-12-18 Ｊｍｔｃエンザイム株式会社 Transformant, method for producing the same, and method for producing lactic acid
AU2016361421B2 (en) 2015-11-24 2021-04-01 Cargill, Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
EP3178313A1 (en) 2015-12-09 2017-06-14 Leibniz-Institut für Pflanzenbiochemie Gene and protein for the synthesis of oxidized zingiberene derivatives
RU2614233C1 (en) * 2015-12-22 2017-03-23 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Yeast transformant schizosaccharomyces pombe, producing lactic acid (versions) method for production thereof (versions), method for lactic acid microbiological synthesis using this transformant
RU2650669C1 (en) * 2016-12-21 2018-04-16 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Schizosaccharomyces pombe strain - lactic acid producer
RU2652877C9 (en) * 2016-12-21 2018-06-26 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) Method for modification of yeast schizosaccharomyces pombe by means of the cre-lox system of bacteriophage p1, transformant that is obtained in such a method
KR102140596B1 (en) 2018-04-17 2020-08-04 에스케이이노베이션 주식회사 Novel Promotor from Organic Acid Resistant Yeast and Method for Expressing Target Gene Using The Same
CN109280652A (en) * 2018-09-13 2019-01-29 四川自豪时代药业有限公司 A kind of fermentation process in high density of external source height expression lactic dehydrogenase enzyme mutant
KR20200040017A (en) * 2018-10-08 2020-04-17 에스케이이노베이션 주식회사 Recombinant Acid Resistant Yeast Inhibited alcohol production and Method for Preparing Lactic Acid Using The Same
EP4025700A1 (en) 2019-09-05 2022-07-13 Leibniz-Institut für Pflanzenbiochemie (IPB) Nucleic acids, proteins and processes for producing piperine and other amides
KR20210041903A (en) 2019-10-08 2021-04-16 에스케이이노베이션 주식회사 Recombinant Acid Resistant Yeast Inhibited Lactate Metabolism and Alcohol Production and Method for Preparing Lactic Acid Using The Same
KR20210158676A (en) 2020-06-24 2021-12-31 에스케이이노베이션 주식회사 Recombinant Acid Resistant Yeast Having Improbed Lactic Acid Productivity
WO2023004336A1 (en) * 2021-07-22 2023-01-26 Archer Daniels Midland Company A genetically engineered yeast producing lactic acid

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US5817478A (en) 1993-10-05 1998-10-06 Asahi Glass Company Ltd. Multicloning vector, expression vector and production of foreign proteins by using the expression vector
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EP0773296B1 (en) 1995-02-03 2006-09-13 Asahi Glass Company Ltd. Secretory signal gene and expression vector having the same
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IT1294728B1 (en) 1997-09-12 1999-04-12 Biopolo S C A R L YEAST STRAWS FOR THE REPRODUCTION OF LACTIC ACID
EP1223219A3 (en) 1997-10-31 2002-10-23 Asahi Glass Company Ltd. Inducible promoter and secretion signal for use in schizosaccharomyces pombe, expression vector containing them and their use
US20070031950A1 (en) 1998-09-11 2007-02-08 Winkler Aaron A Production of D-lactic acid with yeast
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JP2001204464A (en) 2000-01-27 2001-07-31 Toyota Motor Corp Method of producing lactic acid
US7141410B2 (en) * 2000-11-22 2006-11-28 Natureworks Llc Methods and materials for the production of organic products in cells of Candida species
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US20050112737A1 (en) * 2003-11-20 2005-05-26 A. E. Staley Manufacturing Co. Lactic acid producing yeast
JP5248735B2 (en) 2004-01-19 2013-07-31 旭硝子株式会社 Yeast transformation method
JP2006006271A (en) * 2004-06-29 2006-01-12 Toyota Central Res & Dev Lab Inc Lactic acid-producing yeast and lactic acid producing method
EP2182060B1 (en) 2005-08-03 2012-10-24 Asahi Glass Company, Limited Transformed yeast host cells and method of producing foreign protein
EP1930423A4 (en) 2005-08-29 2009-12-09 Asahi Glass Co Ltd Expression vector, transformant having the expression vector introduced therein, and process for production of heterologous protein
WO2007063919A1 (en) * 2005-11-29 2007-06-07 Asahi Glass Company, Limited Method of modifying chromosome
JP5329055B2 (en) 2006-07-24 2013-10-30 東レ株式会社 Yeast having mutant pyruvate decarboxylase 5 gene and method for producing lactic acid
JP2011507532A (en) * 2007-12-23 2011-03-10 ジーヴォ，インコーポレイテッド Yeast organisms producing isobutanol in high yield

Also Published As

Publication number Publication date
EP2468860B1 (en) 2015-11-25
US20120214214A1 (en) 2012-08-23
KR20120051705A (en) 2012-05-22
JP5772594B2 (en) 2015-09-02
US9284561B2 (en) 2016-03-15
WO2011021629A1 (en) 2011-02-24
JPWO2011021629A1 (en) 2013-01-24
EP2468860A9 (en) 2012-12-26
EP2468860A4 (en) 2013-04-03
EP2468860A1 (en) 2012-06-27
CN102498209A (en) 2012-06-13

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