GB9901072D0 - Methods for detecting changes to a macromolecular component of a cell - Google Patents

Methods for detecting changes to a macromolecular component of a cell

Info

Publication number
GB9901072D0
GB9901072D0 GBGB9901072.0A GB9901072A GB9901072D0 GB 9901072 D0 GB9901072 D0 GB 9901072D0 GB 9901072 A GB9901072 A GB 9901072A GB 9901072 D0 GB9901072 D0 GB 9901072D0
Authority
GB
United Kingdom
Prior art keywords
state
macromolecular component
bound
cell
sensor molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB9901072.0A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cancer Research Horizons Ltd
Original Assignee
Imperial Cancer Research Technology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imperial Cancer Research Technology Ltd filed Critical Imperial Cancer Research Technology Ltd
Priority to GBGB9901072.0A priority Critical patent/GB9901072D0/en
Publication of GB9901072D0 publication Critical patent/GB9901072D0/en
Priority to JP2000595150A priority patent/JP2002535655A/en
Priority to DE60009661T priority patent/DE60009661D1/en
Priority to PCT/GB2000/000118 priority patent/WO2000043780A1/en
Priority to AT00900695T priority patent/ATE263971T1/en
Priority to EP20000900695 priority patent/EP1145004B1/en
Priority to AU30623/00A priority patent/AU3062300A/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Burglar Alarm Systems (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)

Abstract

A method of detecting the state, or the change in state, of a macromolecular component of a cell which can exist in a first state or a second state wherein in its first state the macromolecular component is substantially incapable of being bound by a sensor molecule and in its second state the macromolecular component is able to be bound by the sensor molecule, the method comprising the steps of (1) providing a sample comprising the macromolecular component wherein the macromolecular component is bound to a donor fluorophore; (2) contacting the sample provided in (1) with a sensor molecule which is able to bind the macromolecular component in its second state but is substantially incapable of binding the macromolecular component in its first state wherein the sensor molecule is bound to an acceptor molecule; and (3) detecting fluorescence resonance energy transfer (FRET) between the donor fluorophore bound to the macromolecular component and the acceptor molecule bound to the sensor molecule wherein FRET is measured by the decrease in the fluorescence lifetime of the donor fluorophore.
GBGB9901072.0A 1999-01-19 1999-01-19 Methods for detecting changes to a macromolecular component of a cell Ceased GB9901072D0 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
GBGB9901072.0A GB9901072D0 (en) 1999-01-19 1999-01-19 Methods for detecting changes to a macromolecular component of a cell
JP2000595150A JP2002535655A (en) 1999-01-19 2000-01-19 Methods for detecting changes to cellular components
DE60009661T DE60009661D1 (en) 1999-01-19 2000-01-19 METHOD FOR DETECTING CHANGES IN CELLULAR COMPONENTS
PCT/GB2000/000118 WO2000043780A1 (en) 1999-01-19 2000-01-19 Methods for detecting changes to cellular components
AT00900695T ATE263971T1 (en) 1999-01-19 2000-01-19 METHOD FOR DETECTING CHANGES IN CELLULAR COMPONENTS
EP20000900695 EP1145004B1 (en) 1999-01-19 2000-01-19 Methods for detecting changes to cellular components
AU30623/00A AU3062300A (en) 1999-01-19 2000-01-19 Methods for detecting changes to cellular components

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9901072.0A GB9901072D0 (en) 1999-01-19 1999-01-19 Methods for detecting changes to a macromolecular component of a cell

Publications (1)

Publication Number Publication Date
GB9901072D0 true GB9901072D0 (en) 1999-03-10

Family

ID=10846082

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB9901072.0A Ceased GB9901072D0 (en) 1999-01-19 1999-01-19 Methods for detecting changes to a macromolecular component of a cell

Country Status (7)

Country Link
EP (1) EP1145004B1 (en)
JP (1) JP2002535655A (en)
AT (1) ATE263971T1 (en)
AU (1) AU3062300A (en)
DE (1) DE60009661D1 (en)
GB (1) GB9901072D0 (en)
WO (1) WO2000043780A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0007915D0 (en) * 2000-03-31 2000-05-17 Univ Bristol Protein kinase assay
WO2002027031A2 (en) * 2000-09-28 2002-04-04 Cellomics, Inc. Methods and reagents for live-cell gene expression quantification
AT410601B (en) * 2000-12-29 2003-06-25 Hoffmann La Roche SENSOR FOR LUMINESCENCE-OPTICAL DETERMINATION OF ANALYTICAL AND REAGENT THAT WORKS ACCORDING TO THE FRET PRINCIPLE
JP3888974B2 (en) * 2001-03-23 2007-03-07 独立行政法人科学技術振興機構 Protein Phosphorylation / Dephosphorylation Visualization Probe and Method for Detecting and Quantifying Protein Phosphorylation / Dephosphorylation Using the Probe
FR2826666B1 (en) * 2001-07-02 2004-07-02 Novaleads METHOD FOR SELECTING BIO-ACTIVE AGENTS BY LUMINESCENCE COUPLING AND LIVING CELLULAR SYSTEM FOR CARRYING OUT SAID METHOD
JP2005507650A (en) 2001-08-01 2005-03-24 セロミックス インコーポレイテッド Assays for novel fusion proteins and molecular binding
US20030129770A1 (en) * 2001-09-28 2003-07-10 Fernandez Salvador M. Method to improve sensitivity of molecular binding assays using phase-sensitive luminescence detection
GB0208989D0 (en) * 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring enzyme activity
JP2007504445A (en) 2003-08-26 2007-03-01 ブルーシフト・バイオテクノロジーズ・インコーポレーテッド Time-dependent fluorescence measurement
AU2004284456B2 (en) 2003-10-21 2012-04-19 Orion Genomics Llc Methods for quantitative determination of methylation density in a DNA locus
EP1774024A4 (en) 2004-07-02 2012-04-04 Blueshift Biotechnologies Inc Exploring fluorophore microenvironments
US20080311047A1 (en) * 2005-11-16 2008-12-18 Thijs Kaper Multimetric Biosensors and Methods of Using Same
US9250243B2 (en) 2006-09-21 2016-02-02 Nestec S.A. Drug selection for lung cancer therapy using antibody-based arrays
BRPI0717416A2 (en) 2006-09-21 2013-11-12 Prometheus Lab Inc METHOD FOR PERFORMING A HIGH PRODUCTIVITY COMPLEX IMMUNOASON, AND
WO2008065540A2 (en) * 2006-11-27 2008-06-05 Cis Bio International Method for the detection of post-translational modifications
EP2169404B1 (en) * 2007-06-18 2016-01-27 Chugai Seiyaku Kabushiki Kaisha METHOD FOR MEASURING BONDING ACTIVITY OF ANTIBODY WITH AN ANTIGEN AND WITH AN Fc RECEPTOR
CN101688836B (en) * 2007-08-30 2012-08-29 三井造船株式会社 FRET detection method and device
MX2010009140A (en) 2008-02-25 2010-12-20 Prometheus Lab Inc Drug selection for breast cancer therapy using antibody-based arrays.
ES2627909T3 (en) 2009-07-15 2017-08-01 Diatech Holdings, Inc. Drug selection for the treatment of gastric cancer using antibody-based matrices
US8950687B2 (en) 2010-09-21 2015-02-10 Honeywell International Inc. Remote control of an HVAC system that uses a common temperature setpoint for both heat and cool modes
US9719995B2 (en) 2011-02-03 2017-08-01 Pierian Holdings, Inc. Drug selection for colorectal cancer therapy using receptor tyrosine kinase profiling
ES2553456T3 (en) 2011-09-02 2015-12-09 Nestec S.A. Profile path protein profiling to determine therapeutic efficacy
JP7080813B2 (en) * 2015-12-10 2022-06-06 シティ・オブ・ホープ Cell-permeable cyanide-binding antibody

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1179600E (en) * 1996-06-04 2005-08-31 Univ Utah Res Found HIBRIDATION MONITORING DURING CPR
PT1199564E (en) * 1997-04-07 2004-10-29 Bioimage A S A method for tracing substances which has an effect on the intra-cellular translocation

Also Published As

Publication number Publication date
EP1145004A1 (en) 2001-10-17
EP1145004B1 (en) 2004-04-07
JP2002535655A (en) 2002-10-22
ATE263971T1 (en) 2004-04-15
AU3062300A (en) 2000-08-07
DE60009661D1 (en) 2004-05-13
WO2000043780A1 (en) 2000-07-27

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)