GB2256197B - Yeast as host for expression of heterologous glycosyl transferase enzymes - Google Patents

Yeast as host for expression of heterologous glycosyl transferase enzymes

Info

Publication number
GB2256197B
GB2256197B GB9208211A GB9208211A GB2256197B GB 2256197 B GB2256197 B GB 2256197B GB 9208211 A GB9208211 A GB 9208211A GB 9208211 A GB9208211 A GB 9208211A GB 2256197 B GB2256197 B GB 2256197B
Authority
GB
United Kingdom
Prior art keywords
yeast
host
expression
glycosyl transferase
transferase enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB9208211A
Other versions
GB2256197A (en
GB9208211D0 (en
Inventor
Gabriele Watzele
Eric Gilbert Berger
Bernd Meyhack
Manfred Watzele
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Priority to SE9201544A priority Critical patent/SE9201544L/en
Priority to AU17052/92A priority patent/AU655470B2/en
Publication of GB9208211D0 publication Critical patent/GB9208211D0/en
Priority to BE9200491A priority patent/BE1005579A5/en
Priority to JP4136822A priority patent/JPH05199871A/en
Priority to HU9201787A priority patent/HU212927B/en
Priority to IL102052A priority patent/IL102052A0/en
Priority to ES09201117A priority patent/ES2046118B1/en
Priority to FI922515A priority patent/FI922515A/en
Priority to MX9202583A priority patent/MX9202583A/en
Priority to GR920100250A priority patent/GR920100250A/en
Priority to AT0112792A priority patent/AT401940B/en
Priority to CA002070057A priority patent/CA2070057A1/en
Priority to KR1019920009287A priority patent/KR920021709A/en
Priority to DK071992A priority patent/DK71992A/en
Priority to LU88123A priority patent/LU88123A1/en
Priority to ITRM920411A priority patent/IT1255044B/en
Priority to PT100545A priority patent/PT100545A/en
Priority to NO92922134A priority patent/NO922134L/en
Priority to NZ242958A priority patent/NZ242958A/en
Priority to IE921769A priority patent/IE69059B1/en
Publication of GB2256197A publication Critical patent/GB2256197A/en
Application granted granted Critical
Publication of GB2256197B publication Critical patent/GB2256197B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GB9208211A 1991-05-31 1992-04-14 Yeast as host for expression of heterologous glycosyl transferase enzymes Expired - Fee Related GB2256197B (en)

Priority Applications (20)

Application Number Priority Date Filing Date Title
SE9201544A SE9201544L (en) 1991-05-31 1992-05-15 MAKE SUBSTANTIAL GYCOSYL TRANSFER PHASES
AU17052/92A AU655470B2 (en) 1991-05-31 1992-05-20 Improved process for the production of glycosyltransferases
BE9200491A BE1005579A5 (en) 1991-05-31 1992-05-27 METHOD FOR PRODUCING glycosyltransferases, VECTOR HYBRID AND YEAST STRAINS USED FOR THAT PURPOSE.
JP4136822A JPH05199871A (en) 1991-05-31 1992-05-28 Improved production of glycosyltransferase
HU9201787A HU212927B (en) 1991-05-31 1992-05-28 Recombinant process for the production of glycosil-transpherases and method for producing hybrid vectors and transformed yeast strains suitable for it
AT0112792A AT401940B (en) 1991-05-31 1992-05-29 METHOD FOR PRODUCING GLYCOSYL TRANSFERASES
LU88123A LU88123A1 (en) 1991-05-31 1992-05-29 IMPROVED METHOD FOR PRODUCING GLYCOSYL TRANSFERASES
FI922515A FI922515A (en) 1991-05-31 1992-05-29 FOERBAETTRAD FOERFARANDE FOER ATT PRODUCERA GLYKOSYLTRANSFERASER
MX9202583A MX9202583A (en) 1991-05-31 1992-05-29 IMPROVED PROCEDURE FOR THE PRODUCTION OF GLYCOSYL TRANSFERS.
GR920100250A GR920100250A (en) 1991-05-31 1992-05-29 Improved process for the production of glycosyltransferases.
IL102052A IL102052A0 (en) 1991-05-31 1992-05-29 Process for the production of glycosyltransferases
CA002070057A CA2070057A1 (en) 1991-05-31 1992-05-29 Process for the production of glycosyltransferases
KR1019920009287A KR920021709A (en) 1991-05-31 1992-05-29 Improved Method of Making Glycosyltransferases
DK071992A DK71992A (en) 1991-05-31 1992-05-29 PROCEDURE FOR THE PREPARATION OF GLYCOSYL TRANSFER
ES09201117A ES2046118B1 (en) 1991-05-31 1992-05-29 IMPROVED PROCEDURE FOR THE PRODUCTION OF GLYCOSYL TRANSFERS.
ITRM920411A IT1255044B (en) 1991-05-31 1992-05-29 PROCEDURE FOR PRODUCING GLYCOSYLTRANSFERASE, HYBRID YEAR CARRIER IN IT EMPLOYED AND YEAST STOCK TRANSFORMED WITH USE OF HYBRID VECTOR
PT100545A PT100545A (en) 1991-05-31 1992-05-29 IMPROVED PROCESS FOR THE PRODUCTION OF GLYCOSYLTRANSFERASES
NO92922134A NO922134L (en) 1991-05-31 1992-05-29 PROCEDURE FOR PREPARING GLYCOSYL TRANSPARASE
NZ242958A NZ242958A (en) 1991-05-31 1992-05-29 Production of glycosyltransferases
IE921769A IE69059B1 (en) 1991-05-31 1992-07-01 Yeast as host for expression of heterologous glycosyl transferase enzymes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP91810414 1991-05-31
EP92810167 1992-03-04

Publications (3)

Publication Number Publication Date
GB9208211D0 GB9208211D0 (en) 1992-05-27
GB2256197A GB2256197A (en) 1992-12-02
GB2256197B true GB2256197B (en) 1995-11-22

Family

ID=26130266

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9208211A Expired - Fee Related GB2256197B (en) 1991-05-31 1992-04-14 Yeast as host for expression of heterologous glycosyl transferase enzymes

Country Status (6)

Country Link
KR (1) KR920021709A (en)
CH (1) CH685057A5 (en)
DE (1) DE4217616A1 (en)
FR (1) FR2677040B1 (en)
GB (1) GB2256197B (en)
NL (1) NL9200943A (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993018157A1 (en) * 1992-03-09 1993-09-16 The Regents Of The University Of California Compositions and methods for the identification and synthesis of sialyltransferases
EP1278881B1 (en) 2000-04-13 2009-12-30 Biotica Technology Limited Hybrid glycosylated products and their production and use
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
US7214660B2 (en) 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
PL1615945T3 (en) 2003-04-09 2012-03-30 Ratiopharm Gmbh Glycopegylation methods and proteins/peptides produced by the methods
US9005625B2 (en) 2003-07-25 2015-04-14 Novo Nordisk A/S Antibody toxin conjugates
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
EP1771066A2 (en) 2004-07-13 2007-04-11 Neose Technologies, Inc. Branched peg remodeling and glycosylation of glucagon-like peptide-1 glp-1
WO2006050247A2 (en) 2004-10-29 2006-05-11 Neose Technologies, Inc. Remodeling and glycopegylation of fibroblast growth factor (fgf)
NZ556436A (en) 2005-01-10 2010-11-26 Biogenerix Ag Glycopegylated granulocyte colony stimulating factor
EP2386571B1 (en) 2005-04-08 2016-06-01 ratiopharm GmbH Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
US20070105755A1 (en) 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
US20090048440A1 (en) 2005-11-03 2009-02-19 Neose Technologies, Inc. Nucleotide Sugar Purification Using Membranes
DE102005060813A1 (en) * 2005-12-20 2007-06-28 Rheinisch-Westfälische Technische Hochschule Aachen Selective labeling of glycans of antibodies with modified nucleotide sugars as substrates of recombinant galactosyltransferases
ES2516694T3 (en) 2006-07-21 2014-10-31 Ratiopharm Gmbh Glycosylation of peptides through glycosylation sequences with O-binding
WO2008057683A2 (en) 2006-10-03 2008-05-15 Novo Nordisk A/S Methods for the purification of polypeptide conjugates
BRPI0809670A8 (en) 2007-04-03 2018-12-18 Biogenerix Ag methods to increase stem cell production, to increase the number of granulocytes in an individual, to prevent, treat and alleviate myelosuppression that results from cancer therapy, to treat a condition in an individual, to treat neutropenia and thrombocytopenia in a mammal, to expand hematopoietic stem cells in culture, to increase hematopoiesis in an individual, to increase the number of hematopoietic progenitor cells in an individual, and to provide stable bone marrow graft, and, oral dosage form.
CN101778859B (en) 2007-06-12 2014-03-26 诺和诺德公司 Improved process for the production of nucleotide sugars
CN103497246B (en) 2008-02-27 2016-08-10 诺沃—诺迪斯克有限公司 The Factor VlII molecule puted together
CN113481180A (en) * 2021-07-05 2021-10-08 吉林大学 Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction
CN114369584B (en) * 2022-02-06 2023-09-22 北京睿脉医药科技有限公司 Recombinant human source fucosyltransferase variant and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007000A2 (en) * 1988-12-13 1990-06-28 La Jolla Cancer Research Foundation Nucleotides encoding human b1, 4-galactosyltransferase and uses thereof
WO1991006635A1 (en) * 1989-10-24 1991-05-16 Regents Of The University Of California Method for producing secretable glycosyltransferases and other golgi processing enzymes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
DE69131925D1 (en) * 1990-02-14 2000-02-24 Univ Michigan Ann Arbor METHODS AND PRODUCTS FOR THE SYNTHESIS OF OLIGOSACCHARID STRUCTURES ON GLYCOPROTEINS, GLYCOLIPIDS OR AS FREE MOLECULES
EP0485532A1 (en) * 1990-04-27 1992-05-20 Biogen, Inc. Fucosyl transferases involved in adhesion molecule expression

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007000A2 (en) * 1988-12-13 1990-06-28 La Jolla Cancer Research Foundation Nucleotides encoding human b1, 4-galactosyltransferase and uses thereof
WO1991006635A1 (en) * 1989-10-24 1991-05-16 Regents Of The University Of California Method for producing secretable glycosyltransferases and other golgi processing enzymes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Biochemistry Vol 29,1990,pages 2349-2356 *
Enzyme Nomenclature 1984, Academic Press Inc. (1984) *
J.Biol.Chem.,Vol.265, No.11, page 6225-6234, (1990) *
J.Biol.Chem.,Vol.265, pages 1615-1622, (1990) *
J.Cell.Biol.,Vol.107, 10A, (1988) *
Proc.Natl.Acad.Sci.(USA), Vol.87, pages 6674-6678, (1990) *
Yeast Biotechnology,pages 401-429,Edit.D.R.Berry, I.Russell,G.G.Stewart. Allen & Unwin, (1987) *

Also Published As

Publication number Publication date
NL9200943A (en) 1992-12-16
GB2256197A (en) 1992-12-02
KR920021709A (en) 1992-12-18
GB9208211D0 (en) 1992-05-27
DE4217616A1 (en) 1992-12-03
CH685057A5 (en) 1995-03-15
FR2677040A1 (en) 1992-12-04
FR2677040B1 (en) 1995-07-13

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19990414