GB1432039A - Method of treating microorganisms - Google Patents

Method of treating microorganisms

Info

Publication number
GB1432039A
GB1432039A GB5176473A GB5176473A GB1432039A GB 1432039 A GB1432039 A GB 1432039A GB 5176473 A GB5176473 A GB 5176473A GB 5176473 A GB5176473 A GB 5176473A GB 1432039 A GB1432039 A GB 1432039A
Authority
GB
United Kingdom
Prior art keywords
treatment
slurry
rupturing
cells
mins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5176473A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dainippon Seito KK
Original Assignee
Dainippon Seito KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP11202672A external-priority patent/JPS4971186A/ja
Priority claimed from JP11202772A external-priority patent/JPS5530834B2/ja
Application filed by Dainippon Seito KK filed Critical Dainippon Seito KK
Publication of GB1432039A publication Critical patent/GB1432039A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/18—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/22—Working-up of proteins for foodstuffs by texturising
    • A23J3/225—Texturised simulated foods with high protein content
    • A23J3/227—Meat-like textured foods

Abstract

1432039 Extracting contents of microorganism cells DAI NIPPON SUGAR MFG CO Ltd 7 Nov 1973 [10 Nov 1972 (2)] 51764/73 Headings A2B and C6F [Also in Division C6] An aqueous slurry of microorganisms containing 2-32% dry weight of living cells is treated with at least one agent to weaken' the cell walls, the agent being an acid, alkali, or hydrophilic organic solvent, for 20 mins-2 hrs at <70‹C for pH 0.5-4.0, for 10 mins- 16 hrs at 25-65‹C for pH 7.0-10.5 or for 4 mins-6 hrs at 0-30‹C for pH 10.5-12.0, and then the cells are mechanically ruptured using a grinder or roll mill. The microorganism may be a yeast (e.g. Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, Saccharomyces carlsbergensis, Candida tropicalis, Candida lipolytica, or Rodotorula glutinis), or a bacterium (e.g. Bacillus subtilis or Corynebacterium sp.) or an alga (e.g. Arthrospira, Spirulina, Scenedestius sp. and Chlorella vulgaris). The alkali for treatment at pH 7.0-10.5 may be ammonia or a hydroxide, carbonate or alkaline lactate, citrate, succinate, or acetate of Na, K, NH 3 , Mg or Ca, and for treatment at pH 10.5-12.9 NaOH or KOH may be used, whilst for acid treatment hydrochloric, phosphoric, acetic, lactic, or citric acid may be used. Treatment at pH 7.5-9.5 may be carried out in the presence of ethyl acetate, toluene, acetone, or an alcohol in an amount of 0.5-3% of the weight of the slurry. Treatment may be effected using acetone, methanol, ethanol, propanol or isopropanol preferably in an amount <2.4 times that of the cell moisture content at <30‹C till the intracellular moisture content is <30% w/w. Preferably the cells are separated from the residual waste liquor before rupturing. Preferred rupturing devices are a ball mill containing glass or ceramic beads, or Zr or Al balls having a size of 0.2-1.5 mm or a sand grinder containing 30-90% v/u sand. The rupturing may be carried out continuously with a slurry input rate of 1.8- 40 times the vessel volume portions. A threeroll, high-speed mill, may be used, the front roll being rotated at 120-350 m/min at 20-40 kg/cm<SP>2</SP> pressure with an input of slurry containing 45-65 wt% moisture. Prior to rupturing the slurry may be mixed with cereal flour, powdered gluten, soya bean protein, sodium alginate or starch. Waste liquor may be retained, adjusted to pH 1-7, and dried as an extract, possibly after treatment at pH 5.0-6.0 with activated carbon.
GB5176473A 1972-11-10 1973-11-07 Method of treating microorganisms Expired GB1432039A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP11202672A JPS4971186A (en) 1972-11-10 1972-11-10
JP11202772A JPS5530834B2 (en) 1972-11-10 1972-11-10

Publications (1)

Publication Number Publication Date
GB1432039A true GB1432039A (en) 1976-04-14

Family

ID=26451284

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5176473A Expired GB1432039A (en) 1972-11-10 1973-11-07 Method of treating microorganisms

Country Status (1)

Country Link
GB (1) GB1432039A (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0008728A2 (en) * 1978-08-26 1980-03-19 Deutsche Hefewerke GmbH Preparation of yeast autolysate
EP0173924A2 (en) * 1984-09-01 1986-03-12 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Mechanical process for dislodging bacterial cells for the isolation of peptides prepared by recombinant techniques
WO1988008881A1 (en) * 1987-05-11 1988-11-17 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
EP0342892A1 (en) * 1988-05-17 1989-11-23 Schering Corporation Extraction of human interleukin-4 from bacteria
WO2005016024A1 (en) * 2003-08-15 2005-02-24 Grain Processing Corporation Method for dissociation of cells
WO2015001261A1 (en) * 2013-07-04 2015-01-08 Roquette Freres Optimised method for breaking chlorella walls by mechanical crushing
US9386774B2 (en) * 2014-12-16 2016-07-12 Heliae Development, Llc Application of mixotrophic chlorella for the improved yield and quality of solanaceae plants
US11102985B2 (en) 2017-11-10 2021-08-31 Heliae Development, Llc Biomass compositions and methods for making the same
CN114249409A (en) * 2021-11-26 2022-03-29 河海大学 Pre-oxidation coupling pressurizing device and method for river and lake blue algae treatment

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0008728A2 (en) * 1978-08-26 1980-03-19 Deutsche Hefewerke GmbH Preparation of yeast autolysate
EP0008728A3 (en) * 1978-08-26 1980-04-16 Deutschen Hefewerke Gestellschaft Mit Beschrankter Haftung Yeast autolysate, its preparation and its application in food and fodder
EP0173924A2 (en) * 1984-09-01 1986-03-12 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Mechanical process for dislodging bacterial cells for the isolation of peptides prepared by recombinant techniques
EP0173924A3 (en) * 1984-09-01 1988-02-03 Boehringer Ingelheim International G.M.B.H Mechanical process for dislodging bacterial cells for the isolation of peptides prepared by recombinant techniques
WO1988008881A1 (en) * 1987-05-11 1988-11-17 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
EP0291294A1 (en) * 1987-05-11 1988-11-17 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
US5136024A (en) * 1987-05-11 1992-08-04 Schering Corporation Extraction of granulocyte macrophage colony stimulating factor from bacteria
US4958007A (en) * 1988-05-17 1990-09-18 Schering-Plough Corp. Extraction of human interleukin-4- from bacteria
WO1989011541A1 (en) * 1988-05-17 1989-11-30 Schering Corporation Extraction of human interleukin-4 from bacteria
EP0342892A1 (en) * 1988-05-17 1989-11-23 Schering Corporation Extraction of human interleukin-4 from bacteria
WO2005016024A1 (en) * 2003-08-15 2005-02-24 Grain Processing Corporation Method for dissociation of cells
US7425439B2 (en) 2003-08-15 2008-09-16 Grain Processing Corporation Method for dissociation of cells
WO2015001261A1 (en) * 2013-07-04 2015-01-08 Roquette Freres Optimised method for breaking chlorella walls by mechanical crushing
FR3008001A1 (en) * 2013-07-04 2015-01-09 Roquette Freres OPTIMIZED METHOD OF BREAKING CHLORELLA WALLS BY MECHANICAL MILLING
US10465159B2 (en) 2013-07-04 2019-11-05 Corbion Biotech, Inc. Optimised method for breaking chlorella walls by mechanical crushing
US9386774B2 (en) * 2014-12-16 2016-07-12 Heliae Development, Llc Application of mixotrophic chlorella for the improved yield and quality of solanaceae plants
US11102985B2 (en) 2017-11-10 2021-08-31 Heliae Development, Llc Biomass compositions and methods for making the same
CN114249409A (en) * 2021-11-26 2022-03-29 河海大学 Pre-oxidation coupling pressurizing device and method for river and lake blue algae treatment

Also Published As

Publication number Publication date
AU6234573A (en) 1975-05-15

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee