GB1385320A - Cholesterol assay - Google Patents

Cholesterol assay

Info

Publication number
GB1385320A
GB1385320A GB3306874A GB3306874A GB1385320A GB 1385320 A GB1385320 A GB 1385320A GB 3306874 A GB3306874 A GB 3306874A GB 3306874 A GB3306874 A GB 3306874A GB 1385320 A GB1385320 A GB 1385320A
Authority
GB
United Kingdom
Prior art keywords
amount
cholesterol
hydrogen peroxide
cholestenone
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3306874A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK filed Critical National Research Development Corp UK
Priority to GB3306874A priority Critical patent/GB1385320A/en
Publication of GB1385320A publication Critical patent/GB1385320A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1385320 Determination of cholesterol NATIONAL RESEARCH DEVELOPMENT CORP 18 Sept 1972 [22 Sept 1971 19 June 1972] 33069/74 and 33068/74 Divided out of 1385319 Heading G1B A method of assaying for cholesterol in a liquid (e.g. serum) comprises incubating the liquid with an enzyme preparation capable of oxidizing the cholesterol into #4-cholestenone and hydrogen peroxide and determining the amount of cholesterol present by measuring the amount of hydrogen peroxide or #4-cholestenone produced or the amount of oxygen absorbed in the reaction. Preferably the enzyme is obtained from a microorganism of the group Mycobacterium rhodocrous e.g. Nocardia species NCIB 10554 or NCIB 10555 wherein the enzyme preparation has a cholesterol oxidase specific activity of at least 1 unit per 5mg of protein nitrogen and a potency of at least 10<SP>-2</SP> units/ml. The amount of hydrogen peroxide may be measured fluorimetrically or by means of a chromogenic reagent, for example by means of quadrivalent titanium and xylenol oranges or by reaction of the hydrogen peroxide with 4-aminophenazone in the presence of phenol and peroxidase, the product being measured colorimetrically. Other reagents are mentioned. The amount of oxygen taken up may be measured by means of an oxygen electrode and the amount of #<SP>4</SP>-cholestenone may be determined by its carbonyl absorption or a derivative of #4-cholestenone such as its 2, 4 diphenyl hydrazone or iso nicotinic acid hydrazone is formed and is measured colorimetrically. A kit for use in assaying the amount in a liquid comprises in association: (i) an enzyme preparation capable of oxidizing cholesterol into #4-cholestenone and hydrogen peroxide preferably as defined above and (ii) at least one reagent which is capable of being used in the determination of the amount in which hydrogen peroxide or #<SP>4</SP>-cholestenone is formed. The reagent (ii) may be a reagent which takes part in a reaction by means of which hydrogen peroxide may be determined fluorimetrically or colorimetrically and the kit may comprise additionally at least one reagent capable of being used in hydrolyzing serum for serum cholesterol assays.
GB3306874A 1971-09-22 1972-06-19 Cholesterol assay Expired GB1385320A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB3306874A GB1385320A (en) 1971-09-22 1972-06-19 Cholesterol assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB3306974 1971-09-22
GB3306874A GB1385320A (en) 1971-09-22 1972-06-19 Cholesterol assay

Publications (1)

Publication Number Publication Date
GB1385320A true GB1385320A (en) 1975-02-26

Family

ID=26261702

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3306874A Expired GB1385320A (en) 1971-09-22 1972-06-19 Cholesterol assay

Country Status (1)

Country Link
GB (1) GB1385320A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0016947A1 (en) * 1979-04-04 1980-10-15 Roche Diagnostics GmbH Process and reagent for the enzymatic determination of enzymatic substrates
US4247631A (en) * 1979-01-31 1981-01-27 Millipore Corporation Reagent and method for the analytic determination of hydrogen peroxide
US6737275B2 (en) 2001-02-05 2004-05-18 The Board Of Regents For Oklahoma State University Direct serum lipids assays for evaluation of disease states

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4247631A (en) * 1979-01-31 1981-01-27 Millipore Corporation Reagent and method for the analytic determination of hydrogen peroxide
FR2476129A1 (en) * 1979-01-31 1981-08-21 Millipore Corp METHOD FOR PRODUCING DYE FOR DETECTING THE PRESENCE OF HYDROGEN PEROXIDE IN AQUEOUS MEDIUM, METHOD FOR ENZYMATIC DETERMINATION OF THE QUANTITY OF A COMPONENT IN A BODILY FLUID, AND REACTIVE SYSTEM FOR USE
EP0016947A1 (en) * 1979-04-04 1980-10-15 Roche Diagnostics GmbH Process and reagent for the enzymatic determination of enzymatic substrates
US6737275B2 (en) 2001-02-05 2004-05-18 The Board Of Regents For Oklahoma State University Direct serum lipids assays for evaluation of disease states

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Legal Events

Date Code Title Description
PS Patent sealed
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PE20 Patent expired after termination of 20 years