GB1356794A - Apparatus and method for the production of a single cell suspen sion from tissue - Google Patents
Apparatus and method for the production of a single cell suspen sion from tissueInfo
- Publication number
- GB1356794A GB1356794A GB5526870A GB5526870A GB1356794A GB 1356794 A GB1356794 A GB 1356794A GB 5526870 A GB5526870 A GB 5526870A GB 5526870 A GB5526870 A GB 5526870A GB 1356794 A GB1356794 A GB 1356794A
- Authority
- GB
- United Kingdom
- Prior art keywords
- tissue
- cells
- filter
- trypsin
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Mechanical Engineering (AREA)
- Cell Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
1356794 Tissue cells DEFENCE SECRETARY OF STATE FOR 21 Feb 1972 [20 Nov 1970] 55268/70 Heading C6F [Also in Division B1] A method for the production of single tissue cells from subdivided tissue (chicken embryo, rabbit, kidney, brain) suspended with concomitant red blood cells in isotonic saline solution in a container 1, Fig. 1, involves adding isotonic solution through line 4 and removing isotonic solution and red bloood cells through rotary filter 19, hollow shaft 15 and line 14 under the suction created by a peristaltic pump (not shown). Thereafter, the flow of liquid is stopped while the tissue is enzymatically disaggregated by trypsin and the single tissue cells produced are likewise removed through the filter. The filter element has a pore size which permits only particles having a mean diameter up to three times and generally about twice the diameter of red blood cells in the tissue suspension to be drawn through. The single cells are subsequently recovered from suspension by centrifugation. Preferably a collagen-digesting enzyme, such as pepsin and/or deoxyribonuclease, is used with the trypsin in order to digest the gelatinous fibrin which would otherwise obstruct the filter.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB5526870A GB1356794A (en) | 1970-11-20 | 1970-11-20 | Apparatus and method for the production of a single cell suspen sion from tissue |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB5526870A GB1356794A (en) | 1970-11-20 | 1970-11-20 | Apparatus and method for the production of a single cell suspen sion from tissue |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1356794A true GB1356794A (en) | 1974-06-12 |
Family
ID=10473435
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB5526870A Expired GB1356794A (en) | 1970-11-20 | 1970-11-20 | Apparatus and method for the production of a single cell suspen sion from tissue |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1356794A (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2290497A1 (en) * | 1974-11-07 | 1976-06-04 | Merck & Co Inc | APPARATUS FOR PREPARING INSULATED CELLS FROM TISSUES |
US4350768A (en) * | 1980-09-19 | 1982-09-21 | Bristol Myers Company | Method for preparing single cell suspension |
FR2512459A1 (en) * | 1981-09-04 | 1983-03-11 | Inst Nat Sante Rech Med | Isolating cells from tissue esp. for neuro:biological culture - using fluid shear forces which retain morphology and metabolism |
EP0224962A1 (en) * | 1985-11-25 | 1987-06-10 | De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur | Apparatus for the continuous cultivation of microorganisms in a culture liquid |
US5372945A (en) * | 1985-06-06 | 1994-12-13 | Alchas; Paul G. | Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells to produce endothelial cell product |
FR2729398A1 (en) * | 1995-01-17 | 1996-07-19 | Hospal R & D Int | ORGAN DIGESTION DEVICE AND METHOD FOR ISOLATING CELLS OR AMAS FROM CELLS OF BIOLOGICAL INTEREST FROM AN ORGAN |
FR2925066A1 (en) * | 2007-12-18 | 2009-06-19 | Bertin Technologies Soc Par Ac | DEVICE FOR MILLING A BIOLOGICAL SAMPLE |
EP2330181A1 (en) * | 2002-04-08 | 2011-06-08 | Octane Biotech Inc. | Automated Tissue Engineering System |
WO2011145075A3 (en) * | 2010-05-20 | 2012-04-26 | Carlo Tremolada | Device and method for preparing tissue, particularly adipose tissue |
US11371018B2 (en) | 2017-09-01 | 2022-06-28 | Octane Biotech Inc. | End-to-end cell therapy automation |
EP3171152B1 (en) * | 2015-11-19 | 2023-01-25 | Miltenyi Biotec B.V. & Co. KG | Process and device for isolating cells from biological tissue |
US11597905B2 (en) | 2018-12-28 | 2023-03-07 | Octane Biotech Inc. | Cell culture and tissue engineering systems with controlled environmental zones |
US11714096B2 (en) | 2018-12-21 | 2023-08-01 | Octane Biotech Inc. | Carousel for modular biologic production units |
US11718833B2 (en) | 2018-12-21 | 2023-08-08 | Lonza Walkersville, Inc. | Automated production of viral vectors |
US11773365B2 (en) | 2019-02-08 | 2023-10-03 | Lonza Walkersville, Inc. | Cell concentration methods and devices for use in automated bioreactors |
-
1970
- 1970-11-20 GB GB5526870A patent/GB1356794A/en not_active Expired
Cited By (40)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2290497A1 (en) * | 1974-11-07 | 1976-06-04 | Merck & Co Inc | APPARATUS FOR PREPARING INSULATED CELLS FROM TISSUES |
US4028190A (en) * | 1974-11-07 | 1977-06-07 | Merck & Co., Inc. | Apparatus for preparing isolated cells from tissue |
US4350768A (en) * | 1980-09-19 | 1982-09-21 | Bristol Myers Company | Method for preparing single cell suspension |
FR2512459A1 (en) * | 1981-09-04 | 1983-03-11 | Inst Nat Sante Rech Med | Isolating cells from tissue esp. for neuro:biological culture - using fluid shear forces which retain morphology and metabolism |
US5372945A (en) * | 1985-06-06 | 1994-12-13 | Alchas; Paul G. | Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells to produce endothelial cell product |
US4888294A (en) * | 1985-11-25 | 1989-12-19 | Nederlanden Vertegenwoordigd | Apparatus and method for the continuous cultivation of microorganisms in a culture liquid |
EP0224962A1 (en) * | 1985-11-25 | 1987-06-10 | De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur | Apparatus for the continuous cultivation of microorganisms in a culture liquid |
FR2729398A1 (en) * | 1995-01-17 | 1996-07-19 | Hospal R & D Int | ORGAN DIGESTION DEVICE AND METHOD FOR ISOLATING CELLS OR AMAS FROM CELLS OF BIOLOGICAL INTEREST FROM AN ORGAN |
EP0723009A1 (en) * | 1995-01-17 | 1996-07-24 | HOSPAL R&D Int. | A device for organ digestion and a procedure for the isolation of biologically interesting cells or cell-clusters from an organ |
US8492140B2 (en) | 2002-04-08 | 2013-07-23 | Octane Biotech Inc. | Automated tissue engineering system |
US10844338B1 (en) | 2002-04-08 | 2020-11-24 | Octane Biotech Inc. | Automated tissue engineering system |
EP2330181A1 (en) * | 2002-04-08 | 2011-06-08 | Octane Biotech Inc. | Automated Tissue Engineering System |
US10723986B2 (en) | 2002-04-08 | 2020-07-28 | Octane Biotech Inc. | Automated tissue engineering system |
US9701932B2 (en) | 2002-04-08 | 2017-07-11 | Octane Biotech Inc. | Automated tissue engineering system |
US9534195B2 (en) | 2002-04-08 | 2017-01-03 | Octane Biotech Inc. | Automated tissue engineering system |
WO2009103868A1 (en) * | 2007-12-18 | 2009-08-27 | Bertin Technologies | Device for grinding a biological sample |
FR2925066A1 (en) * | 2007-12-18 | 2009-06-19 | Bertin Technologies Soc Par Ac | DEVICE FOR MILLING A BIOLOGICAL SAMPLE |
WO2011145075A3 (en) * | 2010-05-20 | 2012-04-26 | Carlo Tremolada | Device and method for preparing tissue, particularly adipose tissue |
JP2018027086A (en) * | 2010-05-20 | 2018-02-22 | リポジェムズ インターナショナル ソシエタ ペル アチオニ | Device and method for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction |
US10478587B2 (en) | 2010-05-20 | 2019-11-19 | Lipogems International S.P.A. | Device and method for preparing tissue, particularly adipose tissue |
JP2013526868A (en) * | 2010-05-20 | 2013-06-27 | リポジェムズ インターナショナル ソシエタ ア レスポンサビリタ リミタータ | Apparatus and method for preparing tissue for transplantation, in particular adipose tissue, from leaflet fat extracted by liposuction |
CN106834121A (en) * | 2010-05-20 | 2017-06-13 | 里波热姆斯国际股份公司 | Apparatus and method for preparing tissue, particularly adipose tissue |
CN103038333A (en) * | 2010-05-20 | 2013-04-10 | 里波热姆斯国际公司 | Device and method for preparing tissue, particularly adipose tissue |
CN103038333B (en) * | 2010-05-20 | 2017-12-22 | 里波热姆斯国际股份公司 | Method for preparing tissue, particularly adipose tissue |
US11389616B2 (en) | 2010-05-20 | 2022-07-19 | Lipogems International S.P.A. | Method for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction |
US10286177B2 (en) | 2010-05-20 | 2019-05-14 | Lipogems International S.P.A. | Device and kit for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction |
US9044547B2 (en) | 2010-05-20 | 2015-06-02 | Lipogems International S.P.A. | Device and method for preparing tissue, particularly adipose tissue |
EP3106511A1 (en) * | 2010-05-20 | 2016-12-21 | Lipogems International S.p.A. | Device and kit for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction |
AU2011254198B2 (en) * | 2010-05-20 | 2015-03-05 | Lipogems International Spa | Device and method for preparing tissue, particularly adipose tissue |
US10286178B2 (en) | 2010-05-20 | 2019-05-14 | Lipogems International S.P.A. | Method for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction |
JP2016136956A (en) * | 2010-05-20 | 2016-08-04 | リポジェムズ インターナショナル ソシエタ ペル アチオニ | Apparatus and method for preparing tissue for transplant from lobular fat extracted by liposuction, particularly lipid tissue |
EP3171152B1 (en) * | 2015-11-19 | 2023-01-25 | Miltenyi Biotec B.V. & Co. KG | Process and device for isolating cells from biological tissue |
US11447745B2 (en) | 2017-09-01 | 2022-09-20 | Lonza Walkersville, Inc. | End-to-end cell therapy automation |
US11371018B2 (en) | 2017-09-01 | 2022-06-28 | Octane Biotech Inc. | End-to-end cell therapy automation |
US11781113B2 (en) | 2017-09-01 | 2023-10-10 | Lonza Walkersville, Inc. | End-to-end cell therapy automation |
US11827902B2 (en) | 2017-09-01 | 2023-11-28 | Lonza Walkersville, Inc. | End-to-end cell therapy automation |
US11714096B2 (en) | 2018-12-21 | 2023-08-01 | Octane Biotech Inc. | Carousel for modular biologic production units |
US11718833B2 (en) | 2018-12-21 | 2023-08-08 | Lonza Walkersville, Inc. | Automated production of viral vectors |
US11597905B2 (en) | 2018-12-28 | 2023-03-07 | Octane Biotech Inc. | Cell culture and tissue engineering systems with controlled environmental zones |
US11773365B2 (en) | 2019-02-08 | 2023-10-03 | Lonza Walkersville, Inc. | Cell concentration methods and devices for use in automated bioreactors |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |