GB1338579A - Method and apparatus for forming electrophoresis apparatus and the like - Google Patents
Method and apparatus for forming electrophoresis apparatus and the likeInfo
- Publication number
- GB1338579A GB1338579A GB5451170A GB5451170A GB1338579A GB 1338579 A GB1338579 A GB 1338579A GB 5451170 A GB5451170 A GB 5451170A GB 5451170 A GB5451170 A GB 5451170A GB 1338579 A GB1338579 A GB 1338579A
- Authority
- GB
- United Kingdom
- Prior art keywords
- gel
- cover member
- layer
- base member
- cover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/54—Labware with identification means
- B01L3/545—Labware with identification means for laboratory containers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00009—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with a sample supporting tape, e.g. with absorbent zones
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Laminated Bodies (AREA)
Abstract
1338579 Electro-phoresis F R ELEVITCH 16 Nov 1970 [17 Nov 1969] 54511/70 Heading C7B [Also in Divisions B1 and B5] In preparation for the electro-phoresis of a specimen (e.g. of proteins or blood serum) in a gel, the gel is shaped and stored ready for use in apparatus comprising an impermeable base member 11 (which may be in the form of a multiplicity of segments or a continuous strip) and a cover member 10. The gel, which is in the form of a layer of aqueous permeable gel optionally containing reagent, is located between the members and is adherent to the base member, the inner surface of the cover member being uniformly coated with a normally solid thermoplastic essentially hydrocarbon material which is in surface contact with the gel and non-adherent to it. Thereby, the gel can be stored and the cover be easily separated there from when ready to perform the electro-phoresis. The layer of gel may be not more than 1 mm thick and this permits rapid migration under an applied voltage of 10 to 15 volts per cm. In the embodiment shown, the inner surface of cover member 10 has ribs 19 and projections 24a, 24b; in assembling the apparatus the edges of cover 10 are heat or adhesive-sealed to base member 11, gel is poured in through a vent 26 and the vent is capped or sealed. If the base member is a continuous strip it may then be cut into segments. For use, the cover member is stripped off and sample introduced into the wells formed in the gel by ribs 19 and projections 24. The base and/or cover member may be of polystyrene, polyethylene or polypropylene. The inner surface of the cover member may be pre-coated with a paraffin wax solution. The gel formulation may be 10% sucrose, 1 to 1.2% agarose in an aqueous solution of sodium barbital /HCL pH 8.6, optionally containing 0.50% to 0.2% polyvinyl alcohol. Other gels comprise starch, agar or acrylamide. In another embodiment Fig.10 (not shown) a package 110 comprises a plurality of base members each carrying a layer of gel without wells formed in it, one face of each base member being non-adherent to the adjacent gel layer and so forming the cover member for that gel layer. (See Specification 1338580).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1338580D GB1338580A (en) | 1969-11-17 | 1970-11-16 | Apparatus for diffusion analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US87737869A | 1969-11-17 | 1969-11-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1338579A true GB1338579A (en) | 1973-11-28 |
Family
ID=25369861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB5451170A Expired GB1338579A (en) | 1969-11-17 | 1970-11-16 | Method and apparatus for forming electrophoresis apparatus and the like |
Country Status (4)
| Country | Link |
|---|---|
| CA (1) | CA941280A (en) |
| DE (1) | DE2056049A1 (en) |
| FR (1) | FR2069512A5 (en) |
| GB (1) | GB1338579A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999065664A1 (en) * | 1998-06-18 | 1999-12-23 | 3M Innovative Properties Company | Microfluidic articles and method of manufacturing same |
| US6290685B1 (en) | 1998-06-18 | 2001-09-18 | 3M Innovative Properties Company | Microchanneled active fluid transport devices |
| US6381846B2 (en) | 1998-06-18 | 2002-05-07 | 3M Innovative Properties Company | Microchanneled active fluid heat exchanger method |
| US6420622B1 (en) | 1997-08-01 | 2002-07-16 | 3M Innovative Properties Company | Medical article having fluid control film |
| US6431695B1 (en) | 1998-06-18 | 2002-08-13 | 3M Innovative Properties Company | Microstructure liquid dispenser |
| US6454839B1 (en) | 1999-10-19 | 2002-09-24 | 3M Innovative Properties Company | Electrofiltration apparatus |
| US6514412B1 (en) | 1998-06-18 | 2003-02-04 | 3M Innovative Properties Company | Microstructured separation device |
| US6524488B1 (en) | 1998-06-18 | 2003-02-25 | 3M Innovative Properties Company | Method of filtering certain particles from a fluid using a depth loading filtration media |
| US7223364B1 (en) | 1999-07-07 | 2007-05-29 | 3M Innovative Properties Company | Detection article having fluid control film |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3024288C2 (en) * | 1980-06-27 | 1982-03-25 | Europäisches Laboratorium für Molekularbiologie (EMBL), 6900 Heidelberg | Method and device for the production of thin layers |
-
1970
- 1970-11-12 CA CA097,943A patent/CA941280A/en not_active Expired
- 1970-11-13 DE DE19702056049 patent/DE2056049A1/en active Pending
- 1970-11-16 FR FR7041047A patent/FR2069512A5/fr not_active Expired
- 1970-11-16 GB GB5451170A patent/GB1338579A/en not_active Expired
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6420622B1 (en) | 1997-08-01 | 2002-07-16 | 3M Innovative Properties Company | Medical article having fluid control film |
| US7910790B2 (en) | 1997-08-01 | 2011-03-22 | 3M Innovative Properties Company | Medical article having fluid control film |
| US7781639B2 (en) | 1997-08-01 | 2010-08-24 | 3M Innovative Properties Company | Medical article having fluid control film |
| US6514412B1 (en) | 1998-06-18 | 2003-02-04 | 3M Innovative Properties Company | Microstructured separation device |
| US6381846B2 (en) | 1998-06-18 | 2002-05-07 | 3M Innovative Properties Company | Microchanneled active fluid heat exchanger method |
| US6431695B1 (en) | 1998-06-18 | 2002-08-13 | 3M Innovative Properties Company | Microstructure liquid dispenser |
| US6290685B1 (en) | 1998-06-18 | 2001-09-18 | 3M Innovative Properties Company | Microchanneled active fluid transport devices |
| US6375871B1 (en) | 1998-06-18 | 2002-04-23 | 3M Innovative Properties Company | Methods of manufacturing microfluidic articles |
| WO1999065664A1 (en) * | 1998-06-18 | 1999-12-23 | 3M Innovative Properties Company | Microfluidic articles and method of manufacturing same |
| US6524488B1 (en) | 1998-06-18 | 2003-02-25 | 3M Innovative Properties Company | Method of filtering certain particles from a fluid using a depth loading filtration media |
| US6761962B2 (en) | 1998-06-18 | 2004-07-13 | 3M Innovative Properties Company | Microfluidic articles |
| US6867342B2 (en) | 1998-06-18 | 2005-03-15 | 3M Innovative Properties Company | Drug delivery dressing having fluid control film |
| US6907921B2 (en) | 1998-06-18 | 2005-06-21 | 3M Innovative Properties Company | Microchanneled active fluid heat exchanger |
| US7223364B1 (en) | 1999-07-07 | 2007-05-29 | 3M Innovative Properties Company | Detection article having fluid control film |
| US8197775B2 (en) | 1999-07-07 | 2012-06-12 | 3M Innovative Properties Company | Detection article having fluid control film |
| US6471746B2 (en) | 1999-10-19 | 2002-10-29 | 3M Innovative Properties Company | Electrofiltration process |
| US6454839B1 (en) | 1999-10-19 | 2002-09-24 | 3M Innovative Properties Company | Electrofiltration apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CA941280A (en) | 1974-02-05 |
| FR2069512A5 (en) | 1971-09-03 |
| DE2056049A1 (en) | 1971-06-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |