FR3065225B1 - ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS - Google Patents

ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS Download PDF

Info

Publication number
FR3065225B1
FR3065225B1 FR1753233A FR1753233A FR3065225B1 FR 3065225 B1 FR3065225 B1 FR 3065225B1 FR 1753233 A FR1753233 A FR 1753233A FR 1753233 A FR1753233 A FR 1753233A FR 3065225 B1 FR3065225 B1 FR 3065225B1
Authority
FR
France
Prior art keywords
adjuvants
follicles
embryos
cytokines
culture media
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1753233A
Other languages
French (fr)
Other versions
FR3065225A1 (en
Inventor
Patrick Choay
Mohamed Bidri
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Patrick Choay Sas
Original Assignee
Patrick Choay Sas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Patrick Choay Sas filed Critical Patrick Choay Sas
Priority to FR1753233A priority Critical patent/FR3065225B1/en
Priority to PCT/FR2018/000089 priority patent/WO2018189436A1/en
Priority to CN201880037582.7A priority patent/CN110709505A/en
Publication of FR3065225A1 publication Critical patent/FR3065225A1/en
Application granted granted Critical
Publication of FR3065225B1 publication Critical patent/FR3065225B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0609—Oocytes, oogonia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/35—Polyols, e.g. glycerin, inositol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

Abstract

La présente invention a pour objet des compositions utilisables comme adjuvants pour la culture in vitro de cellules, caractérisées en ce qu'elles comprennent au moins deux composés choisis parmi le groupe constitué par un sel de zinc, l'inositol sous l'une quelconque de ses formes stéréoisomériques, la carnitine ou un dérivé de la carnitine, la mélatonine ou un analogue, la cystine sous l'une de ses formes stéréoisomériques ou sous forme de dérivé, la cystéamine ou un sel de cystéamine lesdites compositions étant dépourvues de cytokines. L'invention a également pour objet des procédés de fécondation in vitro et des procédés de maturation de follicules, de cellules germinales mâles, ou d'embryons utilisant de tels adjuvants de milieux de culture, ainsi que des kits pour la mise en œuvre de ces procédés et comprenant un lyophilisat susmentionné et une solution aqueuse, contenant les autres constituants desdits milieux de culture.The subject of the present invention is compositions which can be used as adjuvants for the in vitro culture of cells, characterized in that they comprise at least two compounds chosen from the group consisting of a zinc salt, inositol under any one of its stereoisomeric forms, carnitine or a carnitine derivative, melatonin or an analogue, cystine in one of its stereoisomeric forms or in derivative form, cysteamine or a cysteamine salt, said compositions being devoid of cytokines. The invention also relates to in vitro fertilization processes and maturation processes of follicles, male germ cells, or embryos using such culture media adjuvants, as well as kits for the implementation of these processes and comprising a lyophilisate mentioned above and an aqueous solution, containing the other constituents of said culture media.

FR1753233A 2017-04-12 2017-04-12 ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS Active FR3065225B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR1753233A FR3065225B1 (en) 2017-04-12 2017-04-12 ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS
PCT/FR2018/000089 WO2018189436A1 (en) 2017-04-12 2018-04-12 Cytokine-free adjuvants for cell culture media, in particular for in vitro fertilisation, or for the culture of follicles, male germ cells or embryos
CN201880037582.7A CN110709505A (en) 2017-04-12 2018-04-12 Cytokine-free adjuvants, in particular for in vitro fertilization or for cell culture media for follicles, male germ cells or embryos

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1753233 2017-04-12
FR1753233A FR3065225B1 (en) 2017-04-12 2017-04-12 ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS

Publications (2)

Publication Number Publication Date
FR3065225A1 FR3065225A1 (en) 2018-10-19
FR3065225B1 true FR3065225B1 (en) 2024-04-05

Family

ID=59745993

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1753233A Active FR3065225B1 (en) 2017-04-12 2017-04-12 ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS

Country Status (3)

Country Link
CN (1) CN110709505A (en)
FR (1) FR3065225B1 (en)
WO (1) WO2018189436A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111073848B (en) * 2020-01-02 2023-07-07 佛山辅康生物科技有限公司 Composition for culturing embryo in cleavage stage and embryo culture solution in cleavage stage
CN111280163B (en) * 2020-02-12 2021-11-09 天津博裕力牧科技有限公司 Egg extracting liquid for egg taking of live cattle and preparation method and application thereof
CN111235092B (en) * 2020-03-06 2022-03-25 佛山辅康生物科技有限公司 Blastocyst culture solution and preparation method thereof
CN111548987B (en) * 2020-05-14 2021-07-09 温氏食品集团股份有限公司 Pig oocyte in-vitro maturation culture solution and application thereof
CN112111448B (en) * 2020-08-21 2022-06-10 深圳市弘际生物科技有限责任公司 Improved mesenchymal stem cell culture medium, bone marrow mesenchymal stem cell, and culture method and application thereof
CN114836373B (en) * 2022-05-30 2023-11-10 上海洲康医疗器械有限公司 Egg cell activation culture medium and cultivation method and application thereof
CN115029303B (en) * 2022-06-22 2023-08-15 安徽第肆纪医学科技有限公司 Human immature oocyte IVM energy culture solution for COH period

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR910006124B1 (en) 1987-08-17 1991-08-13 넬슨 리써취 앤드 디벨럽먼트 캄파니 Melatonin analogues
FR2751536B1 (en) 1996-07-25 1998-11-06 Oreal NOVEL FREE ANTI-RADICAL MELATONIN DERIVATIVES AND COMPOSITIONS COMPRISING THE SAME
AUPP421298A0 (en) 1998-06-19 1998-07-09 Fertilitescentrum Ab Method and medium for in vitro culture of human embryos
AU2707100A (en) * 1998-11-30 2000-06-19 Ivf Sciences Colorado, Inc. System and sequential culture media for in vitro fertilization
FR2812004B1 (en) * 2000-07-24 2002-12-27 Ccd Lab CULTURE MEDIA FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERMINAL CELLS OR EMBRYOS
US6552064B2 (en) 2000-09-19 2003-04-22 University Of Iowa Research Foundation Use of melatonin for induction of general anesthesia
US6638966B2 (en) 2000-09-19 2003-10-28 University Of Iowa Research Foundation Use of melatonin analogues for induction of general anesthesia
JP2005518823A (en) * 2002-03-08 2005-06-30 マクギル・ユニバーシテイ In vitro maturation of immature human oocytes
US9861656B2 (en) * 2007-08-08 2018-01-09 Allergy Research Group, Llc Phospholipid compositions and use thereof to enhance spermatozoa motility and viability
ITFI20110271A1 (en) * 2011-12-13 2013-06-14 Lo Li Pharma Srl CULTURAL LANDS FOR THE TREATMENT OF FEMALE RANGE AND EMBRYOS IN MEDICALLY ASSISTED REPRODUCTION TECHNIQUES.
WO2014137733A1 (en) * 2013-03-07 2014-09-12 Cook Medical Technologies Llc Unique buffering system for cell culture media and gamete and embryo culture media and methods
GB2517194A (en) * 2013-08-15 2015-02-18 Cambridge Entpr Ltd Media and methods for culturing embryos and stem cells
WO2015022541A1 (en) * 2013-08-15 2015-02-19 Cambridge Enterprise Limited Media and methods for culturing embryos and stem cells

Also Published As

Publication number Publication date
FR3065225A1 (en) 2018-10-19
WO2018189436A1 (en) 2018-10-18
CN110709505A (en) 2020-01-17

Similar Documents

Publication Publication Date Title
FR3065225B1 (en) ADJUVANTS FREE OF CYTOKINES FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS
MX2022015812A (en) Inhibitors of cysteine proteases and methods of use thereof.
MX2020014315A (en) Heteroaryl compounds for treating huntington's disease.
EA202092001A1 (en) COMPOUNDS FOR TREATMENT OF GENTINGTON'S DISEASE
MX367204B (en) Concentrated personal cleansing compositions and methods.
MX2021004410A (en) Protein tyrosine phosphatase inhibitors.
MX2021013854A (en) Compounds for treating huntington's disease.
EP4306529A3 (en) Fgfr inhibitors and methods of use thereof
EA202091709A1 (en) DNA PC INHIBITORS
AU2020258568A8 (en) CD73 inhibitors
TW200728305A (en) Spiropiperidine beta-sectetase inhibitors for the treatment of alzheimer's disease
ATE538651T1 (en) SPIROPIPERIDINE INHIBITORS OF BETA-SECRETASE FOR THE TREATMENT OF ALZHEIMER'S DISEASE
MA34300B1 (en) HETEROCYCLIC NITROGENIC COMPOUNDS AS PDE10 INHIBITORS
MA35084B1 (en) Composition containing anthraquinone derivatives as growth promoters and antifungal agents
PH12021550382A1 (en) Tetrahydropyridopyrimidine derivatives as ahr modulators
PH12021550028A1 (en) Substituted thiophenecarboxamides and analogues as antibacterials agents
MA34545B1 (en) CO-CRYSTALS AND SALTS OF CCR3 INHIBITORS
MX2022012575A (en) Compounds for treating huntington's disease.
CR20210544A (en) ][1,2,4]thiadiazine 1,1-dioxide derivatives as inhibitors of mrgx2
MX2022001952A (en) Alkynyl quinazoline compounds.
FR3061208B1 (en) ADJUVANTS FOR CELL CULTURE MEDIA, PARTICULARLY FOR IN VITRO FERTILIZATION, OR FOR THE CULTURE OF FOLLICLES, MALE GERM CELLS OR EMBRYOS
MX2020009555A (en) Selective inhibitors of protein arginine methyltransferase 5 (prmt5).
AU2020378127A8 (en) Compounds as CD73 inhibitors
MX2022004394A (en) Estrogen-related receptor alpha (errî±) modulators.
MX2022000165A (en) Estrogen-related receptor alpha (errî±) modulators.

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20181019

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4

PLFP Fee payment

Year of fee payment: 5

PLFP Fee payment

Year of fee payment: 6

PLFP Fee payment

Year of fee payment: 7