FR2799139B1 - BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS. - Google Patents

BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS.

Info

Publication number
FR2799139B1
FR2799139B1 FR9912317A FR9912317A FR2799139B1 FR 2799139 B1 FR2799139 B1 FR 2799139B1 FR 9912317 A FR9912317 A FR 9912317A FR 9912317 A FR9912317 A FR 9912317A FR 2799139 B1 FR2799139 B1 FR 2799139B1
Authority
FR
France
Prior art keywords
amplification
nucleic acids
analysis
microfluidic substrate
analysis device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9912317A
Other languages
French (fr)
Other versions
FR2799139A1 (en
Inventor
Yves Fouillet
Claude Vauchier
Jean Frederic Clerc
Christine Peponnet
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Biodevelopment SAS
Original Assignee
Genset SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR9912317A priority Critical patent/FR2799139B1/en
Application filed by Genset SA filed Critical Genset SA
Priority to US09/627,647 priority patent/US7015030B1/en
Priority to PCT/IB2000/001137 priority patent/WO2001007159A2/en
Priority to EP00949852A priority patent/EP1203096B1/en
Priority to JP2001512031A priority patent/JP2003505059A/en
Priority to AU63109/00A priority patent/AU782726B2/en
Priority to CA002379927A priority patent/CA2379927A1/en
Priority to AT00949852T priority patent/ATE419384T1/en
Priority to DE60041264T priority patent/DE60041264D1/en
Priority to US09/772,280 priority patent/US6977145B2/en
Publication of FR2799139A1 publication Critical patent/FR2799139A1/en
Application granted granted Critical
Publication of FR2799139B1 publication Critical patent/FR2799139B1/en
Priority to US11/197,040 priority patent/US20050282224A1/en
Priority to US11/196,989 priority patent/US20060011478A1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1883—Means for temperature control using thermal insulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
FR9912317A 1999-07-28 1999-10-01 BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS. Expired - Fee Related FR2799139B1 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
FR9912317A FR2799139B1 (en) 1999-10-01 1999-10-01 BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS.
DE60041264T DE60041264D1 (en) 1999-07-28 2000-07-28 Flow microreactor act in the local temperature cycles on a flowing sample
EP00949852A EP1203096B1 (en) 1999-07-28 2000-07-28 Continuous flow micro device in which local temperature cycles act on a flowing sample
JP2001512031A JP2003505059A (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in continuous flow microfluidic devices
AU63109/00A AU782726B2 (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device
CA002379927A CA2379927A1 (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device
US09/627,647 US7015030B1 (en) 1999-07-28 2000-07-28 Microfluidic devices and uses thereof in biochemical processes
PCT/IB2000/001137 WO2001007159A2 (en) 1999-07-28 2000-07-28 Integration of biochemical protocols in a continuous flow microfluidic device
AT00949852T ATE419384T1 (en) 1999-07-28 2000-07-28 FLOW MICROREACTOR IN WHICH LOCAL TEMPERATURE CYCLES ACTION ON A FLOWING SAMPLE
US09/772,280 US6977145B2 (en) 1999-07-28 2001-01-29 Method for carrying out a biochemical protocol in continuous flow in a microreactor
US11/197,040 US20050282224A1 (en) 1999-07-28 2005-08-04 Method for carrying out a biochemical protocol in continuous flow in a microreactor
US11/196,989 US20060011478A1 (en) 1999-07-28 2005-08-04 Integration of biochemical protocols in a continuous flow microfluidic device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9912317A FR2799139B1 (en) 1999-10-01 1999-10-01 BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS.

Publications (2)

Publication Number Publication Date
FR2799139A1 FR2799139A1 (en) 2001-04-06
FR2799139B1 true FR2799139B1 (en) 2002-05-03

Family

ID=9550518

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9912317A Expired - Fee Related FR2799139B1 (en) 1999-07-28 1999-10-01 BIOCHEMICAL ANALYSIS DEVICE COMPRISING A MICROFLUIDIC SUBSTRATE, PARTICULARLY FOR THE AMPLIFICATION OR ANALYSIS OF NUCLEIC ACIDS.

Country Status (1)

Country Link
FR (1) FR2799139B1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002319041A1 (en) * 2001-07-11 2003-01-29 David Erickson Microchannel thermal reactor
GB0304033D0 (en) * 2003-02-21 2003-03-26 Imp College Innovations Ltd Apparatus
US7727477B2 (en) 2004-12-10 2010-06-01 Bio-Rad Laboratories, Inc. Apparatus for priming microfluidics devices with feedback control
US7998708B2 (en) * 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
JP5036377B2 (en) * 2007-04-09 2012-09-26 株式会社日立ソリューションズ Reaction apparatus and reaction chip
FR2930457B1 (en) 2008-04-24 2010-06-25 Commissariat Energie Atomique PROCESS FOR MANUFACTURING RECONFIGURABLE MICROCHANNELS
EP3235568B1 (en) * 2014-12-18 2019-01-30 Ikerlan, S. Coop Disposable device for performing plurality of simultaneous biological experiments in fluidic samples
CN108287185B (en) * 2018-01-09 2024-01-12 南京信息工程大学 Sounding humidity sensor, preparation method, sounding humidity measurement system and sounding humidity measurement method
US11596943B2 (en) * 2018-07-25 2023-03-07 Canon Virginia, Inc. Multi hole inlet structure

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270183A (en) * 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
ATE208658T1 (en) * 1993-07-28 2001-11-15 Pe Corp Ny APPARATUS AND METHOD FOR NUCLEIC ACID DUPLICATION
DE69525036T2 (en) * 1994-11-14 2002-08-01 Univ Pennsylvania DEVICE FOR THE AMPLIFICATION OF POLYNUCLEOTIDES ON A MEASURING SCALE
DE19717085C2 (en) * 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Processes and devices for extremely fast DNA multiplication using polymerase chain reactions (PCR)
EP1054735B1 (en) * 1998-02-11 2003-05-28 Institut Für Physikalische Hochtechnologie E.V. Miniaturized temperature-zone flow reactor
JP4398096B2 (en) * 1998-10-16 2010-01-13 コミツサリア タ レネルジー アトミーク Chemical and / or biochemical analyzer with analytical support

Also Published As

Publication number Publication date
FR2799139A1 (en) 2001-04-06

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Year of fee payment: 17

ST Notification of lapse

Effective date: 20170630